Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0000283

Physical Location & Seq

Genomic Coordinates Forward (+)

1968853 .. 1971701

Gene Structure 2849 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1022600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1977 bp

ATGAATCACCAAAAAATGGAAAGCATGACTACCCTTCTTTTGATTTTCTCCGTTTTTACTCTGCTCAGCTTTCCGACCAGTACGGATGCTGATTACCTTTATCACATCTGCCCAAACACCACCACCTTCACCCCAAACTCCACCTTTCAGTCCAACCTCAACCGCCTCCTCTCCACCCTCTCCTCCAACGCCAACCGCTCCACCGGCTTCTACAACGCCTCCGTCCAAACCCCAAACAACGACGTCTACGGCCTCTTCCTCTGCCGCGGCGACGTCGTCGGCACCGACTCTTGCGAAACCTGTGTCGCCACCGCTACCGCCGAGGCCCCACAACGCTGCCCTGTAGAAAAACAGATGGTGATCTGGTACGACTACTGCATGCTACGCTACTCCAACGAGTCTTTCTTTTCCAGGGCAGACGACAAGCCTTCCGTGTACATGTGGAACACGCAGAATGCAAGTAGTATAACGGACCAAAACCGGTTCAACGAGGTACTGGCAGCTACGGTCACCGAAGTGGCCGCTCAGACTTCCAATAGCAGTCACAAGTTTGCGACGAAAGAAACGAATGTTTCAGGTTCCATTACGCTGTATGGATTTGGGCAGTGCACGCAGGACCTGTCCTCTGGAGATTGCAATTGGTGTCTTCTGGCAGCTGCAACACAAATTCCTAGAACAAGTGCTGGGGGACGAATTCTAAAACCCAGTTGCAATCTTAGGTTTGAAGTTTACCCCTTCTACTCCTCCGCCCCACCGCCGCCAGCCTCCAATAGAGAAGGGCCTAAAGGAAAAAGCAAAACCCCAACTAGTACAATTGTTGCCGTTGTCGTAGCAATATCTGTATCTGTGCTACTTTTCGTTGTGGGTTACTGCTGCATAAATAGGAGAGCGAGAAAGAAGTACAATTATGCAGCAGCAGACGAACTAAGCGGTGAGTATGACATTACTACTGTCGAGTCTTTGCAATTTGATTTTGCTACCATCCAAGAAACTACGAGCAATTTCTCCGATAAGTTAGGCGAAGGTGGTTTCGGTCAAGTTTACAAGGGTACACTTTCAAACGGTCAAGAAGTAGCTGTGAAGAGGCTATCAAGGAACTCTGGCCAAGGCACAGAAGAGTTTAAGAACGAGATGGTATTGGTAGCCAAGCTTCAACACCGAAATTTGGTTAGGGTATTGGGATTTTGCTTGGAAGGCGAAGAAAAAATTCTTGTTTATGAATATGTGCTCAACAAAAGCCTCGACTGTTTCCTATTTGACCCTGAGAAACAGGGGCAATTGGATTGGTCAAGACGTTACAAGATTATTTCAGGAATTGCTAGAGGAATCATGTACTTGCATGAAGATTCCCGACTTAGAATTATACATCGTGATCTCAAAGCTAGCAATATATTGTTAGATGGGGACATGCATCCAAAAATATCTGATTTTGGCATGGCCAGGATCTTTGGGGTTGATCAAACTCAAGCAAATACAAATAGAATTGTGGGAACATATGGTTATATGTCTCCCGAGTATGCAATGCACGGGCAATTTTCTGTCAAGTCCGATATGTATAGTTTCGGTGTTTTAGTGCTAGAAATCATCAGTGGCAAGAAGAACAGTAACTTCTTTCAGACCGATGCAGCTGAGGACCTGGCGAGCCATGCTTGGAAGTTATGGAGAGACGGGACATCTTTGGAATTGTTGGATCCGAGTCTGAGAGACTCTTATTCAAGGACTGAAGTAATCAGATGCATCCACACCGGCTTACTTTGTGTTCAGGAAGATCCAGTTGATAGGCCTATGATGCAATCAGTAGTTCTCATGCTCAATAGTTACTCTGTCAGTCTACCATTACCTAAAGAACCGGCATTTTTCCTCCAGAGCAAAGCGGTGGGGAACCCATCAATGACAACGCTGGAGTCCGATCAATCTATTAGCAAGTCATCTCCGTCCGTTTATGAAGGATCTATCACTGAATTATACCCTAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

659

Amino Acids

73.24

Weight (kDa)

5.9

Isoelectric Point (pI)

41.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	37 - 133	2.2e-27	Salt stress response/antifungal
Stress-antifung	PF01657	152 - 243	8.4e-10	Salt stress response/antifungal
PK_Tyr_Ser-Thr	PF07714	335 - 601	7.4e-48	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	336 - 536	1.7e-44	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000283)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45860 AT4G05200 AT4G05200 AT4G11521 AT4G11530 AT4G23130 AT4G23130 AT4G23130 AT4G23140 AT4G23140 AT4G23150 AT4G23160 AT4G23170 AT4G23180 AT4G23180 AT4G23180 AT4G23230 AT4G23270 AT4G23270 AT4G23270 AT4G23280 AT4G23280 AT4G23280 AT4G23310 AT4G23310
fragaria_vesca	FvH4_3g02772 FvH4_3g02772 FvH4_3g02773 FvH4_3g02773 FvH4_3g02774 FvH4_3g02800 FvH4_3g02820 FvH4_3g02820 FvH4_3g02830
malus_domestica	MD00G1101900.v1.1 MD00G1102100.v1.1 MD00G1102200.v1.1 MD01G1098200.v1.1 MD05G1340000.v1.1 MD05G1340300.v1.1 MD07G1014100.v1.1 MD07G1014300.v1.1 MD07G1014400.v1.1 MD07G1014700.v1.1 MD10G1312900.v1.1 MD11G1022600.v1.1 MD11G1291500.v1.1 MD14G1207100.v1.1 MD16G1096600.v1.1
prunus_persica	Prupe.4G027300_v2.0.a1 Prupe.4G027400_v2.0.a1 Prupe.4G027700_v2.0.a1 Prupe.4G027800_v2.0.a1
pyrus_communis	pycom05g30960 pycom10g00940 pycom10g00950 pycom10g26430 pycom10g26450 pycom10g26460 pycom10g26470 pycom10g26480 pycom10g26490 pycom10g26500 pycom10g26510 pycom10g26570 pycom11g01820 pycom11g04000 pycom14g17130 pycom14g17140 pycom15g28820
rosa_chinensis	RchiOBHm_Chr4g0408141 RchiOBHm_Chr5g0004211 RchiOBHm_Chr5g0004231 RchiOBHm_Chr5g0004241 RchiOBHm_Chr5g0004261
rosa_laevigata	RLG00000008668 RLG00000008672 RLG00000031212 RLG00000031217
rosa_multiflora	Rmu_co8162658.1_g000001 Rmu_co8336297.1_g000001 Rmu_sc0002073.1_g000001 Rmu_sc0004964.1_g000002 Rmu_sc0004964.1_g000003 Rmu_sc0004964.1_g000007 Rmu_sc0006006.1_g000007 Rmu_sc0007868.1_g000015 Rmu_sc0007868.1_g000016 Rmu_sc0007868.1_g000017 Rmu_sc0007868.1_g000021 Rmu_sc0007868.1_g000023 Rmu_sc0019654.1_g000002 Rmu_sc0038540.1_g000001
rosa_roxburghii	Rroxscaffold_1G00071450 Rroxscaffold_1G00071460 Rroxscaffold_1G00071470 Rroxscaffold_1G00071560 Rroxscaffold_1G00071720 Rroxscaffold_1G00071770 Rroxscaffold_5G00352640
rosa_rugosa	Rorug04G0083700 Rorug04G0410700 Rorug04G0410800 Rorug04G0410900
rosa_samantha	Rh4BG145400 Rh4CG154900 Rh4DG141900 Rh5BG037500 Rh5BG037600 Rh5BG037700 Rh5CG041000 Rh5CG041100 Rh5CG041200 Rh5CG041300 Rh5CG041400 Rh5DG037100
rosa_wichuraiana	Rw4G012130 Rw5G003450 Rw5G003460 Rw5G003470 Rw5G003480 Rw5G003640 Rw5G003660 Rw5G003670 Rw5G003680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	246, 276
AccB1I	GGYRCC	1 cut(s)	281
AccB7I	CCANNNNNTGG	1 cut(s)	16
AccBSI	CCGCTC	2 cut(s)	198, 524
AccI	GTMKAC	2 cut(s)	246, 1831
AccII	CGCG	1 cut(s)	267
AclWI	GGATC	5 cut(s)	1451, 1685, 1698, 1763, 1957
AcoI	YGGCCR	3 cut(s)	519, 1102, 1437
AcsI	RAATTY	4 cut(s)	666, 693, 1162, 1206
AcuI	CTGAAG	1 cut(s)	1743
AcyI	GRCGYC	2 cut(s)	243, 273
AfaI	GTAC	8 cut(s)	82, 368, 437, 495, 811, 902, 1051, 1334
AfiI	CCNNNNNNNGG	2 cut(s)	16, 1165
AflIII	ACRYGT	1 cut(s)	438
AgeI	ACCGGT	1 cut(s)	480
AgsI	TTSAA	5 cut(s)	487, 725, 1059, 1154, 1716
AhlI	ACTAGT	1 cut(s)	806
AjnI	CCWGG	3 cut(s)	410, 1439, 1635
AjuI	GAANNNNNNNTTGG	2 cut(s)	386, 418
AleI	CACNNNNGTG	1 cut(s)	515
AluBI	AGCT	7 cut(s)	69, 503, 656, 1076, 1150, 1382, 1628
AluI	AGCT	7 cut(s)	69, 503, 656, 1076, 1150, 1382, 1628
Alw21I	GWGCWC	2 cut(s)	611, 1230
Alw26I	GTCTC	3 cut(s)	1512, 1659, 1698
Alw44I	GTGCAC	1 cut(s)	607
AlwI	GGATC	5 cut(s)	1451, 1685, 1698, 1763, 1957
AlwNI	CAGNNNCTG	1 cut(s)	619
Ama87I	CYCGRG	1 cut(s)	1511
AoxI	GGCC	7 cut(s)	250, 324, 519, 779, 1102, 1437, 1781
ApaLI	GTGCAC	1 cut(s)	607
ApeKI	GCWGC	8 cut(s)	336, 500, 653, 656, 873, 911, 914, 1625
ApoI	RAATTY	4 cut(s)	666, 693, 1162, 1206
ArsI	GACNNNNNNTTYG	2 cut(s)	288, 320
AsiGI	ACCGGT	1 cut(s)	480
Asp700I	GAANNNNTTC	1 cut(s)	1206
AspS9I	GGNCC	5 cut(s)	325, 472, 616, 779, 1633
AsuHPI	GGTGA	4 cut(s)	121, 370, 502, 944
AsuNHI	GCTAGC	1 cut(s)	1382
AvaI	CYCGRG	1 cut(s)	1511
AvaII	GGWCC	3 cut(s)	472, 616, 1633
BaeGI	GKGCMC	1 cut(s)	611
BalI	TGGCCA	2 cut(s)	1104, 1439
BamHI	GGATCC	1 cut(s)	1690
BanI	GGYRCC	1 cut(s)	281
BarI	GAAGNNNNNNTAC	2 cut(s)	1134, 1166
BbsI	GAAGAC	1 cut(s)	638
Bbv12I	GWGCWC	2 cut(s)	611, 1230
BbvCI	CCTCAGC	1 cut(s)	1629
BbvI	GCAGC	8 cut(s)	323, 512, 643, 665, 860, 923, 926, 1637
BccI	CCATC	5 cut(s)	349, 989, 1126, 1394, 1894
BceAI	ACGGC	2 cut(s)	265, 806
BciT130I	CCWGG	3 cut(s)	412, 1441, 1637
BclI	TGATCA	1 cut(s)	1456
BcoDI	GTCTC	3 cut(s)	1512, 1659, 1698
BcuI	ACTAGT	1 cut(s)	806
BfaI	CTAG	6 cut(s)	672, 807, 1320, 1383, 1577, 1971
BfmI	CTRYAG	1 cut(s)	342
BlpI	GCTNAGC	1 cut(s)	65
Bme1390I	CCNGG	3 cut(s)	412, 1441, 1637
Bme18I	GGWCC	3 cut(s)	472, 616, 1633
BmeT110I	CYCGRG	1 cut(s)	1511
BmgT120I	GGNCC	5 cut(s)	325, 472, 616, 779, 1633
BmiI	GGNNCC	5 cut(s)	283, 327, 580, 1692, 1883
BmrFI	CCNGG	3 cut(s)	412, 1441, 1637
BmrI	ACTGGG	1 cut(s)	699
BmsI	GCATC	6 cut(s)	76, 1420, 1612, 1724, 1746, 1779
BmtI	GCTAGC	1 cut(s)	1386
BmuI	ACTGGG	1 cut(s)	699
BpiI	GAAGAC	1 cut(s)	638
BpmI	CTGGAG	3 cut(s)	648, 1847, 1922
Bpu10I	CCTNAGC	1 cut(s)	1629
Bpu1102I	GCTNAGC	1 cut(s)	65
BpuEI	CTTGAG	1 cut(s)	1449
BsaBI	GATNNNNATC	1 cut(s)	359
BsaHI	GRCGYC	2 cut(s)	243, 273
BsaJI	CCNNGG	4 cut(s)	265, 321, 411, 1105
BsaWI	WCCGGW	1 cut(s)	480
BsaXI	ACNNNNNCTCC	2 cut(s)	203, 233
Bsc4I	CCNNNNNNNGG	2 cut(s)	16, 1165
Bse118I	RCCGGY	4 cut(s)	203, 480, 1745, 1849
Bse1I	ACTGG	4 cut(s)	78, 501, 705, 1772
Bse3DI	GCAATG	1 cut(s)	1527
Bse8I	GATNNNNATC	1 cut(s)	359
BseBI	CCWGG	3 cut(s)	412, 1441, 1637
BseDI	CCNNGG	4 cut(s)	265, 321, 411, 1105
BseGI	GGATG	4 cut(s)	91, 981, 1411, 1737
BseJI	GATNNNNATC	1 cut(s)	359
BseLI	CCNNNNNNNGG	2 cut(s)	16, 1165
BseMI	GCAATG	1 cut(s)	1527
BseMII	CTCAG	5 cut(s)	79, 539, 1254, 1620, 1691
BseNI	ACTGG	4 cut(s)	78, 501, 705, 1772
BseRI	GAGGAG	3 cut(s)	158, 172, 733
BseSI	GKGCMC	1 cut(s)	611
BseXI	GCAGC	8 cut(s)	323, 512, 643, 665, 860, 923, 926, 1637
BseYI	CCCAGC	1 cut(s)	683
Bsh1236I	CGCG	1 cut(s)	267
BshFI	GGCC	7 cut(s)	252, 326, 521, 781, 1104, 1439, 1783
BshNI	GGYRCC	1 cut(s)	281
BshTI	ACCGGT	1 cut(s)	480
BsiHKAI	GWGCWC	2 cut(s)	611, 1230
BsiHKCI	CYCGRG	1 cut(s)	1511
BsiSI	CCGG	4 cut(s)	204, 481, 1746, 1850
BslFI	GGGAC	3 cut(s)	702, 1418, 1684
BslI	CCNNNNNNNGG	2 cut(s)	16, 1165
BsmAI	GTCTC	3 cut(s)	1512, 1659, 1698
BsmBI	CGTCTC	1 cut(s)	1659
BsmFI	GGGAC	3 cut(s)	702, 1418, 1684
BsmI	GAATGC	1 cut(s)	460
BsnI	GGCC	7 cut(s)	252, 326, 521, 781, 1104, 1439, 1783
BsoBI	CYCGRG	1 cut(s)	1511
Bsp1286I	GDGCHC	2 cut(s)	611, 1230
Bsp1407I	TGTACA	1 cut(s)	435
Bsp143I	GATC	8 cut(s)	360, 1372, 1443, 1456, 1690, 1768, 1909, 1949
Bsp1720I	GCTNAGC	1 cut(s)	65
BspANI	GGCC	7 cut(s)	252, 326, 521, 781, 1104, 1439, 1783
BspCNI	CTCAG	5 cut(s)	78, 538, 1255, 1621, 1692
BspFNI	CGCG	1 cut(s)	267
BspLI	GGNNCC	5 cut(s)	283, 327, 580, 1692, 1883
BspOI	GCTAGC	1 cut(s)	1386
BspPI	GGATC	5 cut(s)	1451, 1685, 1698, 1763, 1957
BspT107I	GGYRCC	1 cut(s)	281
BsrBI	CCGCTC	2 cut(s)	198, 524
BsrDI	GCAATG	1 cut(s)	1527
BsrFI	RCCGGY	4 cut(s)	203, 480, 1745, 1849
BsrGI	TGTACA	1 cut(s)	435
BsrI	ACTGG	4 cut(s)	78, 501, 705, 1772
BssAI	RCCGGY	4 cut(s)	203, 480, 1745, 1849
BssECI	CCNNGG	4 cut(s)	265, 321, 411, 1105
BssMI	GATC	8 cut(s)	360, 1372, 1443, 1456, 1690, 1768, 1909, 1949
BssNI	GRCGYC	2 cut(s)	243, 273
BssT1I	CCWWGG	1 cut(s)	1105
Bst2UI	CCWGG	3 cut(s)	412, 1441, 1637
Bst4CI	ACNGT	5 cut(s)	508, 952, 1064, 1247, 1604
Bst6I	CTCTTC	3 cut(s)	260, 1076, 1110
BstACI	GRCGYC	2 cut(s)	243, 273
BstAUI	TGTACA	1 cut(s)	435
BstC8I	GCNNGC	5 cut(s)	380, 611, 762, 1384, 1642
BstDEI	CTNAG	8 cut(s)	65, 525, 716, 926, 1263, 1355, 1629, 1700
BstDSI	CCRYGG	1 cut(s)	265
BstEII	GGTNACC	1 cut(s)	508
BstF5I	GGATG	4 cut(s)	91, 981, 1411, 1737
BstFNI	CGCG	1 cut(s)	267
BstKTI	GATC	8 cut(s)	363, 1375, 1446, 1459, 1693, 1771, 1912, 1952
BstMAI	GTCTC	3 cut(s)	1512, 1659, 1698
BstMBI	GATC	8 cut(s)	360, 1372, 1443, 1456, 1690, 1768, 1909, 1949
BstMWI	GCNNNNNNNGC	6 cut(s)	204, 384, 610, 1194, 1646, 1789
BstNI	CCWGG	3 cut(s)	412, 1441, 1637
BstNSI	RCATGY	3 cut(s)	382, 442, 1411
BstPI	GGTNACC	1 cut(s)	508
BstSCI	CCNGG	3 cut(s)	410, 1439, 1635
BstSFI	CTRYAG	1 cut(s)	342
BstSLI	GKGCMC	1 cut(s)	611
BstUI	CGCG	1 cut(s)	267
BstV1I	GCAGC	8 cut(s)	323, 512, 643, 665, 860, 923, 926, 1637
BstV2I	GAAGAC	1 cut(s)	638
BstX2I	RGATCY	4 cut(s)	1443, 1690, 1768, 1949
BstYI	RGATCY	4 cut(s)	1443, 1690, 1768, 1949
BsuRI	GGCC	7 cut(s)	252, 326, 521, 781, 1104, 1439, 1783
BtgI	CCRYGG	1 cut(s)	265
BtsCI	GGATG	4 cut(s)	91, 981, 1411, 1737
BtsI	GCAGTG	1 cut(s)	611
BtsIMutI	CAGTG	3 cut(s)	611, 1594, 1956
Cac8I	GCNNGC	5 cut(s)	380, 611, 762, 1384, 1642
CaiI	CAGNNNCTG	1 cut(s)	619
Cfr10I	RCCGGY	4 cut(s)	203, 480, 1745, 1849
Cfr13I	GGNCC	5 cut(s)	325, 472, 616, 779, 1633
Cfr42I	CCGCGG	1 cut(s)	268
Csp6I	GTAC	8 cut(s)	81, 367, 436, 494, 810, 901, 1050, 1333
CspAI	ACCGGT	1 cut(s)	480
CviAII	CATG	9 cut(s)	25, 379, 439, 1330, 1340, 1408, 1435, 1646, 1807
CviQI	GTAC	8 cut(s)	81, 367, 436, 494, 810, 901, 1050, 1333
DdeI	CTNAG	8 cut(s)	65, 525, 716, 926, 1263, 1355, 1629, 1700
DpnI	GATC	8 cut(s)	362, 1374, 1445, 1458, 1692, 1770, 1911, 1951
DpnII	GATC	8 cut(s)	360, 1372, 1443, 1456, 1690, 1768, 1909, 1949
EaeI	YGGCCR	3 cut(s)	519, 1102, 1437
Eam1104I	CTCTTC	3 cut(s)	260, 1076, 1110
EarI	CTCTTC	3 cut(s)	260, 1076, 1110
EciI	GGCGGA	1 cut(s)	736
Eco130I	CCWWGG	1 cut(s)	1105
Eco147I	AGGCCT	1 cut(s)	1783
Eco47I	GGWCC	3 cut(s)	472, 616, 1633
Eco57I	CTGAAG	1 cut(s)	1743
Eco88I	CYCGRG	1 cut(s)	1511
Eco91I	GGTNACC	1 cut(s)	508
EcoO109I	RGGNCCY	4 cut(s)	325, 616, 779, 1633
EcoO65I	GGTNACC	1 cut(s)	508
EcoRI	GAATTC	1 cut(s)	693
EcoRII	CCWGG	3 cut(s)	410, 1439, 1635
EcoT14I	CCWWGG	1 cut(s)	1105
EcoT22I	ATGCAT	2 cut(s)	1413, 1739
ErhI	CCWWGG	1 cut(s)	1105
Esp3I	CGTCTC	1 cut(s)	1659
FaeI	CATG	9 cut(s)	28, 382, 442, 1333, 1343, 1411, 1438, 1649, 1810
FaqI	GGGAC	3 cut(s)	702, 1418, 1684
FatI	CATG	9 cut(s)	24, 378, 438, 1329, 1339, 1407, 1434, 1645, 1806
FauNDI	CATATG	1 cut(s)	1495
FbaI	TGATCA	1 cut(s)	1456
FblI	GTMKAC	2 cut(s)	246, 1831
FokI	GGATG	4 cut(s)	98, 968, 1398, 1724
FspBI	CTAG	6 cut(s)	672, 807, 1320, 1383, 1577, 1971
GsaI	CCCAGC	1 cut(s)	687
GsuI	CTGGAG	3 cut(s)	648, 1847, 1922
HaeIII	GGCC	7 cut(s)	252, 326, 521, 781, 1104, 1439, 1783
HapII	CCGG	4 cut(s)	204, 481, 1746, 1850
Hin1I	GRCGYC	2 cut(s)	243, 273
Hin1II	CATG	9 cut(s)	28, 382, 442, 1333, 1343, 1411, 1438, 1649, 1810
HindIII	AAGCTT	1 cut(s)	1148
HinfI	GANTC	9 cut(s)	4, 287, 398, 956, 1326, 1346, 1696, 1706, 1904
HpaII	CCGG	4 cut(s)	204, 481, 1746, 1850
HphI	GGTGA	4 cut(s)	121, 370, 502, 944
Hpy166II	GTNNAC	7 cut(s)	247, 436, 609, 730, 1042, 1052, 1832
Hpy188III	TCNNGA	9 cut(s)	627, 1067, 1290, 1311, 1350, 1370, 1511, 1763, 1864
Hpy8I	GTNNAC	7 cut(s)	247, 436, 609, 730, 1042, 1052, 1832
Hpy99I	CGWCG	5 cut(s)	245, 275, 278, 281, 559
HpyAV	CCTTC	8 cut(s)	44, 136, 438, 745, 770, 1016, 1187, 1940
HpyCH4III	ACNGT	5 cut(s)	508, 952, 1064, 1247, 1604
HpyCH4IV	ACGT	3 cut(s)	243, 273, 1294
HpyF10VI	GCNNNNNNNGC	6 cut(s)	204, 384, 610, 1194, 1646, 1789
HpyF3I	CTNAG	8 cut(s)	65, 525, 716, 926, 1263, 1355, 1629, 1700
HpySE526I	ACGT	3 cut(s)	243, 273, 1294
Hsp92I	GRCGYC	2 cut(s)	243, 273
Hsp92II	CATG	9 cut(s)	28, 382, 442, 1333, 1343, 1411, 1438, 1649, 1810
Ksp22I	TGATCA	1 cut(s)	1456
KspI	CCGCGG	1 cut(s)	268
Kzo9I	GATC	8 cut(s)	360, 1372, 1443, 1456, 1690, 1768, 1909, 1949
LmnI	GCTCC	1 cut(s)	203
Lsp1109I	GCAGC	8 cut(s)	323, 512, 643, 665, 860, 923, 926, 1637
LweI	GCATC	6 cut(s)	76, 1420, 1612, 1724, 1746, 1779
MaeI	CTAG	6 cut(s)	672, 807, 1320, 1383, 1577, 1971
MaeII	ACGT	3 cut(s)	243, 273, 1294
MaeIII	GTNAC	6 cut(s)	508, 542, 866, 1295, 1604, 1817
MalI	GATC	8 cut(s)	362, 1374, 1445, 1458, 1692, 1770, 1911, 1951
MbiI	CCGCTC	2 cut(s)	198, 524
MboI	GATC	8 cut(s)	360, 1372, 1443, 1456, 1690, 1768, 1909, 1949
MboII	GAAGA	8 cut(s)	247, 638, 1093, 1127, 1211, 1355, 1609, 1778
MfeI	CAATTG	3 cut(s)	637, 813, 1277
MflI	RGATCY	4 cut(s)	1443, 1690, 1768, 1949
MhlI	GDGCHC	2 cut(s)	611, 1230
MlsI	TGGCCA	2 cut(s)	1104, 1439
MluNI	TGGCCA	2 cut(s)	1104, 1439
MlyI	GAGTC	6 cut(s)	281, 407, 965, 1700, 1705, 1913
MmeI	TCCRAC	5 cut(s)	98, 177, 210, 417, 1668
Mox20I	TGGCCA	2 cut(s)	1104, 1439
Mph1103I	ATGCAT	2 cut(s)	1413, 1739
MroXI	GAANNNNTTC	1 cut(s)	1206
MscI	TGGCCA	2 cut(s)	1104, 1439
MseI	TTAA	1 cut(s)	1122
MslI	CAYNNNNRTG	1 cut(s)	515
Msp20I	TGGCCA	2 cut(s)	1104, 1439
MspA1I	CMGCKG	3 cut(s)	267, 656, 1628
MspI	CCGG	4 cut(s)	204, 481, 1746, 1850
MspR9I	CCNGG	3 cut(s)	412, 1441, 1637
MunI	CAATTG	3 cut(s)	637, 813, 1277
Mva1269I	GAATGC	1 cut(s)	460
MvaI	CCWGG	3 cut(s)	412, 1441, 1637
MvnI	CGCG	1 cut(s)	267
MwoI	GCNNNNNNNGC	6 cut(s)	204, 384, 610, 1194, 1646, 1789
NdeI	CATATG	1 cut(s)	1495
NdeII	GATC	8 cut(s)	360, 1372, 1443, 1456, 1690, 1768, 1909, 1949
NheI	GCTAGC	1 cut(s)	1382
NlaIII	CATG	9 cut(s)	28, 382, 442, 1333, 1343, 1411, 1438, 1649, 1810
NlaIV	GGNNCC	5 cut(s)	283, 327, 580, 1692, 1883
NmeAIII	GCCGAG	1 cut(s)	346
NmuCI	GTSAC	2 cut(s)	508, 542
NsiI	ATGCAT	2 cut(s)	1413, 1739
NspI	RCATGY	3 cut(s)	382, 442, 1411
OliI	CACNNNNGTG	1 cut(s)	515
PaeI	GCATGC	1 cut(s)	382
PceI	AGGCCT	1 cut(s)	1783
PciI	ACATGT	1 cut(s)	438
PcsI	WCGNNNNNNNCGW	2 cut(s)	282, 563
PctI	GAATGC	1 cut(s)	460
PdmI	GAANNNNTTC	1 cut(s)	1206
PfeI	GAWTC	3 cut(s)	4, 1326, 1346
PflFI	GACNNNGTC	1 cut(s)	275
PflMI	CCANNNNNTGG	1 cut(s)	16
PinAI	ACCGGT	1 cut(s)	480
PleI	GAGTC	6 cut(s)	281, 406, 964, 1700, 1704, 1912
PpsI	GAGTC	6 cut(s)	281, 406, 964, 1700, 1704, 1912
PpuMI	RGGWCCY	2 cut(s)	616, 1633
PscI	ACATGT	1 cut(s)	438
Psp5II	RGGWCCY	2 cut(s)	616, 1633
Psp6I	CCWGG	3 cut(s)	410, 1439, 1635
PspEI	GGTNACC	1 cut(s)	508
PspFI	CCCAGC	1 cut(s)	683
PspGI	CCWGG	3 cut(s)	410, 1439, 1635
PspN4I	GGNNCC	5 cut(s)	283, 327, 580, 1692, 1883
PspPI	GGNCC	5 cut(s)	325, 472, 616, 779, 1633
PspPPI	RGGWCCY	2 cut(s)	616, 1633
PstNI	CAGNNNCTG	1 cut(s)	619
PsuI	RGATCY	4 cut(s)	1443, 1690, 1768, 1949
PsyI	GACNNNGTC	1 cut(s)	275
PvuII	CAGCTG	2 cut(s)	656, 1628
RsaI	GTAC	8 cut(s)	82, 368, 437, 495, 811, 902, 1051, 1334
RsaNI	GTAC	8 cut(s)	81, 367, 436, 494, 810, 901, 1050, 1333
RseI	CAYNNNNRTG	1 cut(s)	515
SacII	CCGCGG	1 cut(s)	268
SaqAI	TTAA	1 cut(s)	1122
Sau3AI	GATC	8 cut(s)	360, 1372, 1443, 1456, 1690, 1768, 1909, 1949
Sau96I	GGNCC	5 cut(s)	325, 472, 616, 779, 1633
SchI	GAGTC	6 cut(s)	281, 407, 965, 1700, 1705, 1913
ScrFI	CCNGG	3 cut(s)	412, 1441, 1637
SduI	GDGCHC	2 cut(s)	611, 1230
SfaNI	GCATC	6 cut(s)	76, 1420, 1612, 1724, 1746, 1779
SfcI	CTRYAG	1 cut(s)	342
Sfr303I	CCGCGG	1 cut(s)	268
SgrBI	CCGCGG	1 cut(s)	268
SinI	GGWCC	3 cut(s)	472, 616, 1633
SmiMI	CAYNNNNRTG	1 cut(s)	515
SmlI	CTYRAG	1 cut(s)	1464
SmoI	CTYRAG	1 cut(s)	1464
SpeI	ACTAGT	1 cut(s)	806
SphI	GCATGC	1 cut(s)	382
SseBI	AGGCCT	1 cut(s)	1783
SspMI	CTAG	6 cut(s)	672, 807, 1320, 1383, 1577, 1971
StuI	AGGCCT	1 cut(s)	1783
StyD4I	CCNGG	3 cut(s)	410, 1439, 1635
StyI	CCWWGG	1 cut(s)	1105
TaaI	ACNGT	5 cut(s)	508, 952, 1064, 1247, 1604
TaiI	ACGT	3 cut(s)	246, 276, 1297
TaqI	TCGA	2 cut(s)	954, 1242
TaqII	GACCGA	2 cut(s)	1022, 1634
TatI	WGTACW	4 cut(s)	435, 809, 900, 1332
TauI	GCSGC	4 cut(s)	267, 270, 524, 760
TfiI	GAWTC	3 cut(s)	4, 1326, 1346
Tru1I	TTAA	1 cut(s)	1122
Tru9I	TTAA	1 cut(s)	1122
TscAI	CASTG	3 cut(s)	611, 1594, 1963
TseFI	GTSAC	2 cut(s)	508, 542
TseI	GCWGC	8 cut(s)	336, 500, 653, 656, 873, 911, 914, 1625
Tsp45I	GTSAC	2 cut(s)	508, 542
TspDTI	ATGAA	4 cut(s)	17, 1233, 1356, 1959
TspGWI	ACGGA	7 cut(s)	40, 98, 211, 421, 485, 1923, 1927
TspRI	CASTG	3 cut(s)	611, 1594, 1963
Tth111I	GACNNNGTC	1 cut(s)	275
Van91I	CCANNNNNTGG	1 cut(s)	16
VneI	GTGCAC	1 cut(s)	607
VpaK11BI	GGWCC	3 cut(s)	472, 616, 1633
XapI	RAATTY	4 cut(s)	666, 693, 1162, 1206
XceI	RCATGY	3 cut(s)	382, 442, 1411
XmiI	GTMKAC	2 cut(s)	246, 1831
XmnI	GAANNNNTTC	1 cut(s)	1206
XspI	CTAG	6 cut(s)	672, 807, 1320, 1383, 1577, 1971
ZraI	GACGTC	2 cut(s)	244, 274
Zsp2I	ATGCAT	2 cut(s)	1413, 1739

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.