MD00G1102100.v1.1

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000283
Physical Location & Seq
Reverse (-)
21528748 .. 21532132
3385 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1102100.v1.1.491

Sequence Viewer

Length: 2040 bp
ATGATACTCAATACAAAAATCTATGATTTTATGATCAGTCATCAAAAAATGAAAAACATGACTACCCTTCTTTTGATTTTCTCCGTTTTTATTCTGCTCAGCTTTCCGACCAGTACGGAAGCTGATTACCTGGTTCACTACTGCCCAAACACCACTACCTTCACCCCAAACTCCACCTTCCAGTCCAACCTCAACCGCCTCCTTTCCACCCTCTCCTCCAACGCTAACCGTTCCACCGGTTTCTACAACGCCACAGTCCAAACCACAAACAACGCCGTCTACGGCCTTTTCCTCTGCCGCGGCGACGTCGCGGGAACCCAAGCTTGCGAAACCTGTGTCGCCACCGCAATTTCCGAGGCCCCACAACGCTGCCCCGTAGAAAAACAGGTGGTGATCGGTTACGACGACTGCATGCTACGCTACTCCAACGAGTCATTCTTCTCCACCGCCACCGAGTCGCCCCGCCTGTACATGTCGAACAATAAAAACGCCACCGACCCGACTCGGTTCAACCAGGTCCTGGCGGCCAGTATGAACATGGTGGCTACCGAGGCTGCCAACGACGCCGACAAGTTCGCGACGAATCAAGCAAATGTAAGCGGGTTGATTTCGGTGTACAGCCTCGGGCAGTGCACGCAGGACCTGTCGGAAGCGGATTGTAACCGCTGTCTTCGGTTGGCCATAGCCCAACTTCCGAATTGCTGTAATGGGAAACTCGGAGGACGAGTTTTGTTTCCGAGTTGTCATGTTAGGTACGAAGTCTACCCCTTCTACGCCAAGAACTCCATGTCAGCACCTGAGCCTGCACCAGGGCCGCCTCCTCCTCTCCCTCCTCCGCCTACAGGAAAAAGCAAAACCCCAACTAGTACAATTGTTGCCATTGCTGTACCAATATCTGTATCTGTGCTACTTTTTGTTGTGGGTTACTGCTGCATAACTAGGAGAGCAAGAAAGAAGTACAATCAAGCAGCAGCAGACGAACCAAGCGGTGAGAATGACATTACTACTGTCGAGTCTTTGCAATTTGATTTTGCTACCATCCAAGCAGCTACGAGCAATTTCTCCGATGATCACAAGTTAGGCGAAGGTGGTTTCGGTCAAGTTTACAAGGGTATACTTTCAAACGGCCAAGAAGTAGCTGTGAAGAGGCTATCAATGAACTCTGACCAAGGCGCGGAAGAGTTTAAGAACGAGATGGTATTGGTAGCCAAGCTTCAACACCGAAATTTGGTTAGGCTCTTGGGATTTTGCTTGGAAGGCGAAGAAAAGATTCTTGTTTATGAATATGTGCCCAACAAAAGCCTTGACCATTTCCTATTTGACCCCGAGAAACAAGGACAATTGGATTGGTCAAGACGTTACAAGATTATTTCAGGAATTGCTCGAGGAATTATGTACCTGCATGAAGATTCCCGACTTAGAATTATACATCGTGATCTCAAAGCCAGCAATATATTGTTAGATGGGGAGATGCATCCAAAAATATCTGATTTTGGCATGGCCAGGATCTTTGGGGTTGATCAAACTCAAGCAAACACAAACAGAATTGTCGGAACATTTGGTTATATGTCTCCCGAGTATGCAATGCACGGACGTTTTTCTGTTAAGTCCGATTTGTATAGTTTTGGTGTTTTAGTGCTAGAAATCATCGCTGGCAAGAAGAACAGTTACTTCCTTCAGACCGATGCAGCTGAGGACCTCCTAAGCCATGCTTGGAAGTTATGGAAAGATGGGGCACCTTTGGAATTGTTGGATCCGTGTCTGAGAGACTCTTATTCGAGGACTGAAGTAATCAGATGCATCCATATCAGCTTACTTTGTGTTCAGGAAGATCCAGCTGACAGGCCTACAATGCAATCAGTAATTCTCATGCTCAATAGCTACTCTCTTACTCTGCCATTACCTCAACAACCGGTATTTTTCCACCAAAGTAGAGCAGTGGGAAACATGTCAGAGGCAACGATGGACTCCGCTCAATCTATCAGCAAGTCATCTCCGTCCGTTTATGAAAAATCTATCACTGAAGTATACCCTAGATAA

Protein Analysis

680

Amino Acids

75.37

Weight (kDa)

6.26

Isoelectric Point (pI)

47.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 48 - 144 5.8e-26 Salt stress response/antifungal
Stress-antifung PF01657 162 - 254 5.9e-12 Salt stress response/antifungal
Pkinase PF00069 355 - 621 1.4e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 357 - 622 1.4e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000283)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45860 AT4G05200 AT4G05200 AT4G11521 AT4G11530 AT4G23130 AT4G23130 AT4G23130 AT4G23140 AT4G23140 AT4G23150 AT4G23160 AT4G23170 AT4G23180 AT4G23180 AT4G23180 AT4G23230 AT4G23270 AT4G23270 AT4G23270 AT4G23280 AT4G23280 AT4G23280 AT4G23310 AT4G23310
fragaria_vesca FvH4_3g02772 FvH4_3g02772 FvH4_3g02773 FvH4_3g02773 FvH4_3g02774 FvH4_3g02800 FvH4_3g02820 FvH4_3g02820 FvH4_3g02830
malus_domestica MD00G1101900.v1.1 MD00G1102100.v1.1 MD00G1102200.v1.1 MD01G1098200.v1.1 MD05G1340000.v1.1 MD05G1340300.v1.1 MD07G1014100.v1.1 MD07G1014300.v1.1 MD07G1014400.v1.1 MD07G1014700.v1.1 MD10G1312900.v1.1 MD11G1022600.v1.1 MD11G1291500.v1.1 MD14G1207100.v1.1 MD16G1096600.v1.1
prunus_persica Prupe.4G027300_v2.0.a1 Prupe.4G027400_v2.0.a1 Prupe.4G027700_v2.0.a1 Prupe.4G027800_v2.0.a1
pyrus_communis pycom05g30960 pycom10g00940 pycom10g00950 pycom10g26430 pycom10g26450 pycom10g26460 pycom10g26470 pycom10g26480 pycom10g26490 pycom10g26500 pycom10g26510 pycom10g26570 pycom11g01820 pycom11g04000 pycom14g17130 pycom14g17140 pycom15g28820
rosa_chinensis RchiOBHm_Chr4g0408141 RchiOBHm_Chr5g0004211 RchiOBHm_Chr5g0004231 RchiOBHm_Chr5g0004241 RchiOBHm_Chr5g0004261
rosa_laevigata RLG00000008668 RLG00000008672 RLG00000031212 RLG00000031217
rosa_multiflora Rmu_co8162658.1_g000001 Rmu_co8336297.1_g000001 Rmu_sc0002073.1_g000001 Rmu_sc0004964.1_g000002 Rmu_sc0004964.1_g000003 Rmu_sc0004964.1_g000007 Rmu_sc0006006.1_g000007 Rmu_sc0007868.1_g000015 Rmu_sc0007868.1_g000016 Rmu_sc0007868.1_g000017 Rmu_sc0007868.1_g000021 Rmu_sc0007868.1_g000023 Rmu_sc0019654.1_g000002 Rmu_sc0038540.1_g000001
rosa_roxburghii Rroxscaffold_1G00071450 Rroxscaffold_1G00071460 Rroxscaffold_1G00071470 Rroxscaffold_1G00071560 Rroxscaffold_1G00071720 Rroxscaffold_1G00071770 Rroxscaffold_5G00352640
rosa_rugosa Rorug04G0083700 Rorug04G0410700 Rorug04G0410800 Rorug04G0410900
rosa_samantha Rh4BG145400 Rh4CG154900 Rh4DG141900 Rh5BG037500 Rh5BG037600 Rh5BG037700 Rh5CG041000 Rh5CG041100 Rh5CG041200 Rh5CG041300 Rh5CG041400 Rh5DG037100
rosa_wichuraiana Rw4G012130 Rw5G003450 Rw5G003460 Rw5G003470 Rw5G003480 Rw5G003640 Rw5G003660 Rw5G003670 Rw5G003680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 309
Acc36I ACCTGC 1 cut(s) 1407
AccB1I GGYRCC 1 cut(s) 1735
AccB7I CCANNNNNTGG 1 cut(s) 520
AccBSI CCGCTC 1 cut(s) 1973
AccI GTMKAC 4 cut(s) 279, 762, 1114, 2028
AccII CGCG 4 cut(s) 300, 311, 578, 1175
AclWI GGATC 4 cut(s) 1514, 1748, 1761, 1826
AcoI YGGCCR 4 cut(s) 525, 678, 1126, 1500
AcsI RAATTY 1 cut(s) 1225
AcuI CTGAAG 2 cut(s) 1661, 1806
AcyI GRCGYC 2 cut(s) 306, 564
AfaI GTAC 8 cut(s) 115, 470, 617, 755, 868, 888, 959, 1397
AfiI CCNNNNNNNGG 5 cut(s) 520, 809, 842, 1174, 1228
AflIII ACRYGT 2 cut(s) 471, 1947
AgeI ACCGGT 2 cut(s) 236, 1912
AgsI TTSAA 3 cut(s) 511, 1122, 1217
AhlI ACTAGT 1 cut(s) 863
AjnI CCWGG 5 cut(s) 129, 513, 519, 808, 1502
AjuI GAANNNNNNNTTGG 2 cut(s) 419, 451
Alw21I GWGCWC 1 cut(s) 635
Alw26I GTCTC 2 cut(s) 1575, 1761
Alw44I GTGCAC 1 cut(s) 631
AlwI GGATC 4 cut(s) 1514, 1748, 1761, 1826
AlwNI CAGNNNCTG 3 cut(s) 520, 643, 797
Ama87I CYCGRG 4 cut(s) 623, 1325, 1383, 1574
AoxI GGCC 8 cut(s) 283, 357, 525, 678, 812, 1126, 1500, 1844
ApaLI GTGCAC 1 cut(s) 631
ApeKI GCWGC 7 cut(s) 369, 554, 930, 968, 971, 1046, 1688
ApoI RAATTY 1 cut(s) 1225
ArsI GACNNNNNNTTYG 2 cut(s) 321, 353
AsiGI ACCGGT 2 cut(s) 236, 1912
Asp700I GAANNNNTTC 1 cut(s) 1269
AspLEI GCGC 1 cut(s) 1175
AspS9I GGNCC 5 cut(s) 358, 517, 640, 812, 1696
AsuHPI GGTGA 3 cut(s) 154, 403, 1001
AvaI CYCGRG 4 cut(s) 623, 1325, 1383, 1574
AvaII GGWCC 3 cut(s) 517, 640, 1696
BaeGI GKGCMC 3 cut(s) 635, 1293, 1738
BalI TGGCCA 2 cut(s) 680, 1502
BamHI GGATCC 1 cut(s) 1753
BanI GGYRCC 1 cut(s) 1735
BarI GAAGNNNNNNTAC 4 cut(s) 1197, 1229, 1652, 1684
BbsI GAAGAC 1 cut(s) 662
Bbv12I GWGCWC 1 cut(s) 635
BbvCI CCTCAGC 1 cut(s) 1692
BbvI GCAGC 7 cut(s) 356, 541, 917, 980, 983, 1058, 1700
BccI CCATC 5 cut(s) 1046, 1189, 1457, 1724, 1957
BceAI ACGGC 3 cut(s) 260, 298, 1141
BciT130I CCWGG 5 cut(s) 131, 515, 521, 810, 1504
BclI TGATCA 3 cut(s) 33, 1069, 1519
BcoDI GTCTC 2 cut(s) 1575, 1761
BcuI ACTAGT 1 cut(s) 863
BfaI CTAG 4 cut(s) 864, 939, 1640, 2034
BfmI CTRYAG 1 cut(s) 840
BfuAI ACCTGC 1 cut(s) 1407
BlpI GCTNAGC 1 cut(s) 98
Bme1390I CCNGG 5 cut(s) 131, 515, 521, 810, 1504
Bme18I GGWCC 3 cut(s) 517, 640, 1696
BmeT110I CYCGRG 4 cut(s) 623, 1325, 1383, 1574
BmgT120I GGNCC 5 cut(s) 358, 517, 640, 812, 1696
BmiI GGNNCC 4 cut(s) 316, 360, 1737, 1755
BmrFI CCNGG 5 cut(s) 131, 515, 521, 810, 1504
BmsI GCATC 5 cut(s) 1461, 1483, 1675, 1787, 1809
BpiI GAAGAC 1 cut(s) 662
Bpu10I CCTNAGC 3 cut(s) 798, 1692, 1703
Bpu1102I GCTNAGC 1 cut(s) 98
BpuEI CTTGAG 1 cut(s) 1512
BsaHI GRCGYC 2 cut(s) 306, 564
BsaJI CCNNGG 6 cut(s) 298, 354, 549, 622, 809, 1168
BsaWI WCCGGW 2 cut(s) 236, 1912
BsaXI ACNNNNNCTCC 2 cut(s) 711, 741
Bsc4I CCNNNNNNNGG 5 cut(s) 520, 809, 842, 1174, 1228
Bse118I RCCGGY 2 cut(s) 236, 1912
Bse1I ACTGG 3 cut(s) 111, 181, 528
Bse3DI GCAATG 2 cut(s) 879, 1590
BseBI CCWGG 5 cut(s) 131, 515, 521, 810, 1504
BseDI CCNNGG 6 cut(s) 298, 354, 549, 622, 809, 1168
BseGI GGATG 3 cut(s) 1038, 1474, 1800
BseLI CCNNNNNNNGG 5 cut(s) 520, 809, 842, 1174, 1228
BseMI GCAATG 2 cut(s) 879, 1590
BseMII CTCAG 4 cut(s) 112, 789, 1683, 1754
BseNI ACTGG 3 cut(s) 111, 181, 528
BseRI GAGGAG 4 cut(s) 205, 810, 813, 822
BseSI GKGCMC 3 cut(s) 635, 1293, 1738
BseXI GCAGC 7 cut(s) 356, 541, 917, 980, 983, 1058, 1700
BsgI GTGCAG 1 cut(s) 789
Bsh1236I CGCG 4 cut(s) 300, 311, 578, 1175
BshFI GGCC 8 cut(s) 285, 359, 527, 680, 814, 1128, 1502, 1846
BshNI GGYRCC 1 cut(s) 1735
BshTI ACCGGT 2 cut(s) 236, 1912
BsiHKAI GWGCWC 1 cut(s) 635
BsiHKCI CYCGRG 4 cut(s) 623, 1325, 1383, 1574
BsiSI CCGG 2 cut(s) 237, 1913
BslI CCNNNNNNNGG 5 cut(s) 520, 809, 842, 1174, 1228
BsmAI GTCTC 2 cut(s) 1575, 1761
BsnI GGCC 8 cut(s) 285, 359, 527, 680, 814, 1128, 1502, 1846
BsoBI CYCGRG 4 cut(s) 623, 1325, 1383, 1574
Bsp1286I GDGCHC 3 cut(s) 635, 1293, 1738
Bsp1407I TGTACA 2 cut(s) 468, 615
Bsp143I GATC 8 cut(s) 33, 393, 1069, 1435, 1506, 1519, 1753, 1831
Bsp1720I GCTNAGC 1 cut(s) 98
Bsp68I TCGCGA 1 cut(s) 578
BspANI GGCC 8 cut(s) 285, 359, 527, 680, 814, 1128, 1502, 1846
BspCNI CTCAG 4 cut(s) 111, 790, 1684, 1755
BspFNI CGCG 4 cut(s) 300, 311, 578, 1175
BspLI GGNNCC 4 cut(s) 316, 360, 1737, 1755
BspMI ACCTGC 1 cut(s) 1407
BspPI GGATC 4 cut(s) 1514, 1748, 1761, 1826
BspT107I GGYRCC 1 cut(s) 1735
BsrBI CCGCTC 1 cut(s) 1973
BsrDI GCAATG 2 cut(s) 879, 1590
BsrFI RCCGGY 2 cut(s) 236, 1912
BsrGI TGTACA 2 cut(s) 468, 615
BsrI ACTGG 3 cut(s) 111, 181, 528
BssAI RCCGGY 2 cut(s) 236, 1912
BssECI CCNNGG 6 cut(s) 298, 354, 549, 622, 809, 1168
BssMI GATC 8 cut(s) 33, 393, 1069, 1435, 1506, 1519, 1753, 1831
BssNAI GTATAC 2 cut(s) 1115, 2029
BssNI GRCGYC 2 cut(s) 306, 564
BssT1I CCWWGG 1 cut(s) 1168
Bst1107I GTATAC 2 cut(s) 1115, 2029
Bst2UI CCWGG 5 cut(s) 131, 515, 521, 810, 1504
Bst4CI ACNGT 4 cut(s) 230, 256, 1009, 1667
Bst6I CTCTTC 2 cut(s) 1139, 1173
BstACI GRCGYC 2 cut(s) 306, 564
BstAUI TGTACA 2 cut(s) 468, 615
BstC8I GCNNGC 6 cut(s) 325, 413, 635, 804, 1447, 1654
BstDEI CTNAG 6 cut(s) 98, 798, 1418, 1692, 1703, 1763
BstDSI CCRYGG 1 cut(s) 298
BstENI CCTNNNNNAGG 1 cut(s) 807
BstF5I GGATG 3 cut(s) 1038, 1474, 1800
BstFNI CGCG 4 cut(s) 300, 311, 578, 1175
BstHHI GCGC 1 cut(s) 1175
BstKTI GATC 8 cut(s) 36, 396, 1072, 1438, 1509, 1522, 1756, 1834
BstMAI GTCTC 2 cut(s) 1575, 1761
BstMBI GATC 8 cut(s) 33, 393, 1069, 1435, 1506, 1519, 1753, 1831
BstMWI GCNNNNNNNGC 6 cut(s) 417, 551, 563, 634, 1257, 1852
BstNI CCWGG 5 cut(s) 131, 515, 521, 810, 1504
BstNSI RCATGY 3 cut(s) 415, 475, 1951
BstSCI CCNGG 5 cut(s) 129, 513, 519, 808, 1502
BstSFI CTRYAG 1 cut(s) 840
BstSLI GKGCMC 3 cut(s) 635, 1293, 1738
BstUI CGCG 4 cut(s) 300, 311, 578, 1175
BstV1I GCAGC 7 cut(s) 356, 541, 917, 980, 983, 1058, 1700
BstV2I GAAGAC 1 cut(s) 662
BstX2I RGATCY 3 cut(s) 1506, 1753, 1831
BstYI RGATCY 3 cut(s) 1506, 1753, 1831
BstZ17I GTATAC 2 cut(s) 1115, 2029
BsuRI GGCC 8 cut(s) 285, 359, 527, 680, 814, 1128, 1502, 1846
BtgI CCRYGG 1 cut(s) 298
BtgZI GCGATG 1 cut(s) 1633
BtsCI GGATG 3 cut(s) 1038, 1474, 1800
BtsI GCAGTG 2 cut(s) 635, 1944
BtsIMutI CAGTG 3 cut(s) 635, 1944, 2019
BtuMI TCGCGA 1 cut(s) 578
BveI ACCTGC 1 cut(s) 1407
Cac8I GCNNGC 6 cut(s) 325, 413, 635, 804, 1447, 1654
CaiI CAGNNNCTG 3 cut(s) 520, 643, 797
CfoI GCGC 1 cut(s) 1175
Cfr10I RCCGGY 2 cut(s) 236, 1912
Cfr13I GGNCC 5 cut(s) 358, 517, 640, 812, 1696
Cfr42I CCGCGG 1 cut(s) 301
CseI GACGC 1 cut(s) 572
CsiI ACCWGGT 2 cut(s) 129, 513
Csp6I GTAC 8 cut(s) 114, 469, 616, 754, 867, 887, 958, 1396
CspAI ACCGGT 2 cut(s) 236, 1912
CviQI GTAC 8 cut(s) 114, 469, 616, 754, 867, 887, 958, 1396
DdeI CTNAG 6 cut(s) 98, 798, 1418, 1692, 1703, 1763
DpnI GATC 8 cut(s) 35, 395, 1071, 1437, 1508, 1521, 1755, 1833
DpnII GATC 8 cut(s) 33, 393, 1069, 1435, 1506, 1519, 1753, 1831
EaeI YGGCCR 4 cut(s) 525, 678, 1126, 1500
Eam1104I CTCTTC 2 cut(s) 1139, 1173
EarI CTCTTC 2 cut(s) 1139, 1173
EciI GGCGGA 1 cut(s) 825
Eco130I CCWWGG 1 cut(s) 1168
Eco147I AGGCCT 1 cut(s) 1846
Eco47I GGWCC 3 cut(s) 517, 640, 1696
Eco57I CTGAAG 2 cut(s) 1661, 1806
Eco88I CYCGRG 4 cut(s) 623, 1325, 1383, 1574
EcoNI CCTNNNNNAGG 1 cut(s) 807
EcoO109I RGGNCCY 4 cut(s) 358, 517, 640, 1696
EcoRII CCWGG 5 cut(s) 129, 513, 519, 808, 1502
EcoT14I CCWWGG 1 cut(s) 1168
EcoT22I ATGCAT 2 cut(s) 1476, 1802
ErhI CCWWGG 1 cut(s) 1168
FalI AAGNNNNNCTT 2 cut(s) 1696, 1728
FauI CCCGC 3 cut(s) 304, 470, 593
FbaI TGATCA 3 cut(s) 33, 1069, 1519
FblI GTMKAC 4 cut(s) 279, 762, 1114, 2028
FokI GGATG 3 cut(s) 1025, 1461, 1787
FspBI CTAG 4 cut(s) 864, 939, 1640, 2034
GlaI GCGC 1 cut(s) 1174
HaeIII GGCC 8 cut(s) 285, 359, 527, 680, 814, 1128, 1502, 1846
HapII CCGG 2 cut(s) 237, 1913
HgaI GACGC 1 cut(s) 572
HhaI GCGC 1 cut(s) 1175
Hin1I GRCGYC 2 cut(s) 306, 564
Hin6I GCGC 1 cut(s) 1173
HinP1I GCGC 1 cut(s) 1173
HindIII AAGCTT 2 cut(s) 321, 1211
HinfI GANTC 9 cut(s) 431, 455, 502, 583, 1013, 1270, 1409, 1769, 1967
HpaII CCGG 2 cut(s) 237, 1913
HphI GGTGA 3 cut(s) 154, 403, 1001
Hpy166II GTNNAC 8 cut(s) 136, 280, 616, 633, 763, 1105, 1115, 2029
Hpy188III TCNNGA 7 cut(s) 577, 1353, 1374, 1413, 1433, 1574, 1826
Hpy8I GTNNAC 8 cut(s) 136, 280, 616, 633, 763, 1105, 1115, 2029
Hpy99I CGWCG 5 cut(s) 308, 311, 407, 566, 583
HpyAV CCTTC 7 cut(s) 77, 169, 187, 778, 1079, 1250, 1685
HpyCH4III ACNGT 4 cut(s) 230, 256, 1009, 1667
HpyCH4IV ACGT 3 cut(s) 306, 1357, 1594
HpyF10VI GCNNNNNNNGC 6 cut(s) 417, 551, 563, 634, 1257, 1852
HpyF3I CTNAG 6 cut(s) 98, 798, 1418, 1692, 1703, 1763
HpySE526I ACGT 3 cut(s) 306, 1357, 1594
Hsp92I GRCGYC 2 cut(s) 306, 564
HspAI GCGC 1 cut(s) 1173
Ksp22I TGATCA 3 cut(s) 33, 1069, 1519
KspI CCGCGG 1 cut(s) 301
Kzo9I GATC 8 cut(s) 33, 393, 1069, 1435, 1506, 1519, 1753, 1831
Lsp1109I GCAGC 7 cut(s) 356, 541, 917, 980, 983, 1058, 1700
LweI GCATC 5 cut(s) 1461, 1483, 1675, 1787, 1809
MabI ACCWGGT 2 cut(s) 129, 513
MaeI CTAG 4 cut(s) 864, 939, 1640, 2034
MaeII ACGT 3 cut(s) 306, 1357, 1594
MaeIII GTNAC 5 cut(s) 398, 659, 923, 1358, 1667
MalI GATC 8 cut(s) 35, 395, 1071, 1437, 1508, 1521, 1755, 1833
MbiI CCGCTC 1 cut(s) 1973
MboI GATC 8 cut(s) 33, 393, 1069, 1435, 1506, 1519, 1753, 1831
MboII GAAGA 8 cut(s) 430, 662, 1156, 1190, 1274, 1418, 1672, 1841
MfeI CAATTG 2 cut(s) 870, 1340
MflI RGATCY 3 cut(s) 1506, 1753, 1831
MhlI GDGCHC 3 cut(s) 635, 1293, 1738
MlsI TGGCCA 2 cut(s) 680, 1502
MluNI TGGCCA 2 cut(s) 680, 1502
MlyI GAGTC 6 cut(s) 440, 464, 496, 1022, 1763, 1961
MmeI TCCRAC 7 cut(s) 131, 210, 243, 450, 627, 1531, 1731
Mox20I TGGCCA 2 cut(s) 680, 1502
Mph1103I ATGCAT 2 cut(s) 1476, 1802
MroXI GAANNNNTTC 1 cut(s) 1269
MscI TGGCCA 2 cut(s) 680, 1502
MseI TTAA 2 cut(s) 1185, 1605
Msp20I TGGCCA 2 cut(s) 680, 1502
MspA1I CMGCKG 4 cut(s) 300, 666, 1691, 1838
MspI CCGG 2 cut(s) 237, 1913
MspR9I CCNGG 5 cut(s) 131, 515, 521, 810, 1504
MunI CAATTG 2 cut(s) 870, 1340
MvaI CCWGG 5 cut(s) 131, 515, 521, 810, 1504
MvnI CGCG 4 cut(s) 300, 311, 578, 1175
MwoI GCNNNNNNNGC 6 cut(s) 417, 551, 563, 634, 1257, 1852
NdeII GATC 8 cut(s) 33, 393, 1069, 1435, 1506, 1519, 1753, 1831
NlaIV GGNNCC 4 cut(s) 316, 360, 1737, 1755
NruI TCGCGA 1 cut(s) 578
NsiI ATGCAT 2 cut(s) 1476, 1802
NspI RCATGY 3 cut(s) 415, 475, 1951
PaeI GCATGC 1 cut(s) 415
PaeR7I CTCGAG 1 cut(s) 1383
PceI AGGCCT 1 cut(s) 1846
PciI ACATGT 2 cut(s) 471, 1947
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PdmI GAANNNNTTC 1 cut(s) 1269
PfeI GAWTC 3 cut(s) 583, 1270, 1409
PflMI CCANNNNNTGG 1 cut(s) 520
PinAI ACCGGT 2 cut(s) 236, 1912
PleI GAGTC 6 cut(s) 439, 463, 496, 1021, 1763, 1961
PpsI GAGTC 6 cut(s) 439, 463, 496, 1021, 1763, 1961
PpuMI RGGWCCY 3 cut(s) 517, 640, 1696
PscI ACATGT 2 cut(s) 471, 1947
Psp5II RGGWCCY 3 cut(s) 517, 640, 1696
Psp6I CCWGG 5 cut(s) 129, 513, 519, 808, 1502
PspGI CCWGG 5 cut(s) 129, 513, 519, 808, 1502
PspN4I GGNNCC 4 cut(s) 316, 360, 1737, 1755
PspPI GGNCC 5 cut(s) 358, 517, 640, 812, 1696
PspPPI RGGWCCY 3 cut(s) 517, 640, 1696
PspXI VCTCGAGB 1 cut(s) 1383
PstNI CAGNNNCTG 3 cut(s) 520, 643, 797
PsuI RGATCY 3 cut(s) 1506, 1753, 1831
PvuII CAGCTG 2 cut(s) 1691, 1838
RruI TCGCGA 1 cut(s) 578
RsaI GTAC 8 cut(s) 115, 470, 617, 755, 868, 888, 959, 1397
RsaNI GTAC 8 cut(s) 114, 469, 616, 754, 867, 887, 958, 1396
SacII CCGCGG 1 cut(s) 301
SaqAI TTAA 2 cut(s) 1185, 1605
Sau3AI GATC 8 cut(s) 33, 393, 1069, 1435, 1506, 1519, 1753, 1831
Sau96I GGNCC 5 cut(s) 358, 517, 640, 812, 1696
SchI GAGTC 6 cut(s) 440, 464, 496, 1022, 1763, 1961
ScrFI CCNGG 5 cut(s) 131, 515, 521, 810, 1504
SduI GDGCHC 3 cut(s) 635, 1293, 1738
SexAI ACCWGGT 2 cut(s) 129, 513
SfaNI GCATC 5 cut(s) 1461, 1483, 1675, 1787, 1809
SfcI CTRYAG 1 cut(s) 840
Sfr274I CTCGAG 1 cut(s) 1383
Sfr303I CCGCGG 1 cut(s) 301
SgrBI CCGCGG 1 cut(s) 301
SinI GGWCC 3 cut(s) 517, 640, 1696
SlaI CTCGAG 1 cut(s) 1383
SmlI CTYRAG 2 cut(s) 1383, 1527
SmoI CTYRAG 2 cut(s) 1383, 1527
SpeI ACTAGT 1 cut(s) 863
SphI GCATGC 1 cut(s) 415
SseBI AGGCCT 1 cut(s) 1846
SspMI CTAG 4 cut(s) 864, 939, 1640, 2034
StuI AGGCCT 1 cut(s) 1846
StyD4I CCNGG 5 cut(s) 129, 513, 519, 808, 1502
StyI CCWWGG 1 cut(s) 1168
TaaI ACNGT 4 cut(s) 230, 256, 1009, 1667
TaiI ACGT 3 cut(s) 309, 1360, 1597
TaqI TCGA 4 cut(s) 476, 1011, 1384, 1778
TaqII GACCGA 2 cut(s) 1085, 1697
TatI WGTACW 4 cut(s) 468, 615, 866, 957
TauI GCSGC 4 cut(s) 300, 303, 527, 817
TfiI GAWTC 3 cut(s) 583, 1270, 1409
Tru1I TTAA 2 cut(s) 1185, 1605
Tru9I TTAA 2 cut(s) 1185, 1605
TscAI CASTG 3 cut(s) 635, 1944, 2026
TseI GCWGC 7 cut(s) 369, 554, 930, 968, 971, 1046, 1688
TspDTI ATGAA 6 cut(s) 65, 548, 1172, 1296, 1419, 2022
TspGWI ACGGA 6 cut(s) 73, 131, 1605, 1746, 1986, 1990
TspRI CASTG 3 cut(s) 635, 1944, 2026
Van91I CCANNNNNTGG 1 cut(s) 520
VneI GTGCAC 1 cut(s) 631
VpaK11BI GGWCC 3 cut(s) 517, 640, 1696
XagI CCTNNNNNAGG 1 cut(s) 807
XapI RAATTY 1 cut(s) 1225
XceI RCATGY 3 cut(s) 415, 475, 1951
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XhoI CTCGAG 1 cut(s) 1383
XmiI GTMKAC 4 cut(s) 279, 762, 1114, 2028
XmnI GAANNNNTTC 1 cut(s) 1269
XspI CTAG 4 cut(s) 864, 939, 1640, 2034
ZraI GACGTC 1 cut(s) 307
Zsp2I ATGCAT 2 cut(s) 1476, 1802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.