RchiOBHm_Chr5g0004261

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000283
Physical Location & Seq
Forward (+)
2719594 .. 2722413
2820 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28552

Sequence Viewer

Length: 2133 bp
ATGCCTTGTTCTCATGTGCTCATGTCCTTTGTGCTGCTCTCCGTTCTTGGTTTCCATAGCCTCAGCGTCGAAGCTGAAATTCCTGACATGTCCCATTTCTGTTCAAACACAACAACTTTCACTCCCAACAGCACCTACGAGTCCAATCTCAATCTCCTCCTCTCCTATCTCACCTCCAACGCCACCAATGACCTCGGATTCCACAACACCACAGTCGGCTCCCAAGACCCCGGCACGACTGTTTACGGTTCATTTAGTTGCCTTGGCGATGTCACCCCTGAAAAATGTCAAGAATGTGTGTCTACTATAGCCAGAGGAGGAGTCCAAAAGTATTGCCCCTTGAGCAAAATCTCATTAATATGGTATGCAGATTGCATGTTACGCTACTCAAACACATCCTTCTTTGGCAACTCGGAGACATCTCCTCAAGTTTCCTTCCTCAACACGGGGAATATCACTGAGCCTGACCGCTTCATGCCGCTGCTGGGACAAACTCTGAAGAGCTTGGTGAGACCTGCTGCTAGTGCACCTTCAGGTGTGAAAAAACTCAAAACAAAAGAAGTGAACTTCACAGATTTTCAGCCGTTGTACACCCTTGTGCAGTGCACACCAGACCTATCGAGCCTAAGCTGTGAAGGTTGTCTTACAGAAGCTATTGCAGCTATTCCAGTTTCTTTTTATGGAAAGCAAGGGGGAGTAGTCTTATGTCCTAGTTGCAATGTTCGATATGAAATCTATCCATTTTACAGAGCTCAAGCTGCCACACCTCCACCAGCGGCATCGCCACCGCCACCATCACTGCTTCTTCCTCCCCCTCCTCCAGGTTCAGTAACGAGATCTCAAGGTAAAATGAATAGCAAGAGTACTATAGCTAATGTAATGTGTTGTCTCACCCCTACAATTGCATTAATCTTAATATTGACTCTATGCAGGAAACGACAAAATCTCATCCTTATCATAGTGGCCATTGTTGTTCCAATTGCCTTGGTGCTTTTCTTGGTAGGCTGCTTTTTTCTAAGAAGCAGGACAAGAAAAAAATATGATGCTTTACAGGGACAGAATGTTGTTGATGGTGCAGGAGATAATGAAATTACTAATGTGGAGTCATTGCGGTTTGATTTTGCTACAATTGAAGCAGCCACAAACCAGTTCTCTGTTAATAATAGATTAGGGGAAGGTGGCTTTGGAGAGGTTTACAAGGGAACACTCCCTAATGGGCAAGAAATAGCTGTGAAGAGGTTATCTAGAAGCTCCGGGCAAGGTGTTGAGCAGTTTAAAAATGAGGTGGTTTTGGTCGCCAAGCTTCTGCACAGAAATTTAGTGAGGCTGCTGGGGTTTTGCTCTGAAGGAGAAGAAAAGATACTTGTTTATGAACTTGTGCAGAACAAAAGCCTTGACCACTTTTTATTCGACTCGGACATTCAAGTAAAGTTGGATTGGTCAAGCAGATACAAGATTATCAGTGGGATTGCTAGAGGCATTCTCTATCTCCATCAAGATTCTCGGCTTAAAATCATACATCGAGATCTTAAAGCGAGCAACGTATTGTTAGATGGGGAAATGAACCCAAAAATAGCAGATTTTGGCATGGCAAGAATATTTGGAGGTGATCAAACACAAGGAAATACCATAAGGATTGTCGGAACTTATGGTTACATGCCTCCGGAGTATGCAATGCACGGACAATTCTCGGTGAAGTCTGATGTGTACAGTCTTGGTGTCTTAATCTTGGAGATTGTAACTGGCGAGAAGAACACTAGCTTCTATCAATCAGACAGCGGCGAAGACCTCTTGACCTATGCTTGGAAACATTGGGGGGATGGGACGCCAGAAGAGTTGTTGGATTCAAATCTGAGAGGTTCTTATTCAAGAAATGAAGTAATCAGATGTATCCATATTGGTTTATTGTGTGTTCAAGAAAATCCAGAGGAGAGACCTACAATGCAAACTATAAATCTCATGCTAAGCAGTTACTCAGTTACTGCGCCATCGCCTCAAAAGCCAGCATTTTGCCTGCATAGTAGAGAAGAGATGAGAATGCCATCAGTAACGTCTGAATCATCAAATCAACACACTAGCACCTCAGTCTGTTCAGTTAATGAAGCATCAATTACTGAATTGTACCCTCGTTAG

Protein Analysis

710

Amino Acids

78.12

Weight (kDa)

6.26

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 34 - 131 2.8e-23 Salt stress response/antifungal
Stress-antifung PF01657 150 - 245 2.2e-08 Salt stress response/antifungal
Pkinase PF00069 385 - 651 1.4e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 386 - 652 2.4e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000283)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45860 AT4G05200 AT4G05200 AT4G11521 AT4G11530 AT4G23130 AT4G23130 AT4G23130 AT4G23140 AT4G23140 AT4G23150 AT4G23160 AT4G23170 AT4G23180 AT4G23180 AT4G23180 AT4G23230 AT4G23270 AT4G23270 AT4G23270 AT4G23280 AT4G23280 AT4G23280 AT4G23310 AT4G23310
fragaria_vesca FvH4_3g02772 FvH4_3g02772 FvH4_3g02773 FvH4_3g02773 FvH4_3g02774 FvH4_3g02800 FvH4_3g02820 FvH4_3g02820 FvH4_3g02830
malus_domestica MD00G1101900.v1.1 MD00G1102100.v1.1 MD00G1102200.v1.1 MD01G1098200.v1.1 MD05G1340000.v1.1 MD05G1340300.v1.1 MD07G1014100.v1.1 MD07G1014300.v1.1 MD07G1014400.v1.1 MD07G1014700.v1.1 MD10G1312900.v1.1 MD11G1022600.v1.1 MD11G1291500.v1.1 MD14G1207100.v1.1 MD16G1096600.v1.1
prunus_persica Prupe.4G027300_v2.0.a1 Prupe.4G027400_v2.0.a1 Prupe.4G027700_v2.0.a1 Prupe.4G027800_v2.0.a1
pyrus_communis pycom05g30960 pycom10g00940 pycom10g00950 pycom10g26430 pycom10g26450 pycom10g26460 pycom10g26470 pycom10g26480 pycom10g26490 pycom10g26500 pycom10g26510 pycom10g26570 pycom11g01820 pycom11g04000 pycom14g17130 pycom14g17140 pycom15g28820
rosa_chinensis RchiOBHm_Chr4g0408141 RchiOBHm_Chr5g0004211 RchiOBHm_Chr5g0004231 RchiOBHm_Chr5g0004241 RchiOBHm_Chr5g0004261
rosa_laevigata RLG00000008668 RLG00000008672 RLG00000031212 RLG00000031217
rosa_multiflora Rmu_co8162658.1_g000001 Rmu_co8336297.1_g000001 Rmu_sc0002073.1_g000001 Rmu_sc0004964.1_g000002 Rmu_sc0004964.1_g000003 Rmu_sc0004964.1_g000007 Rmu_sc0006006.1_g000007 Rmu_sc0007868.1_g000015 Rmu_sc0007868.1_g000016 Rmu_sc0007868.1_g000017 Rmu_sc0007868.1_g000021 Rmu_sc0007868.1_g000023 Rmu_sc0019654.1_g000002 Rmu_sc0038540.1_g000001
rosa_roxburghii Rroxscaffold_1G00071450 Rroxscaffold_1G00071460 Rroxscaffold_1G00071470 Rroxscaffold_1G00071560 Rroxscaffold_1G00071720 Rroxscaffold_1G00071770 Rroxscaffold_5G00352640
rosa_rugosa Rorug04G0083700 Rorug04G0410700 Rorug04G0410800 Rorug04G0410900
rosa_samantha Rh4BG145400 Rh4CG154900 Rh4DG141900 Rh5BG037500 Rh5BG037600 Rh5BG037700 Rh5CG041000 Rh5CG041100 Rh5CG041200 Rh5CG041300 Rh5CG041400 Rh5DG037100
rosa_wichuraiana Rw4G012130 Rw5G003450 Rw5G003460 Rw5G003470 Rw5G003480 Rw5G003640 Rw5G003660 Rw5G003670 Rw5G003680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 523
AccI GTMKAC 1 cut(s) 302
AccIII TCCGGA 1 cut(s) 1663
AciI CCGC 6 cut(s) 469, 479, 776, 788, 1111, 1779
AcoI YGGCCR 1 cut(s) 963
AcsI RAATTY 2 cut(s) 78, 1315
AcuI CTGAAG 3 cut(s) 516, 518, 1365
AcyI GRCGYC 1 cut(s) 1826
AfaI GTAC 4 cut(s) 590, 865, 1709, 2123
AfiI CCNNNNNNNGG 4 cut(s) 445, 485, 821, 1803
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 6 cut(s) 105, 1133, 1424, 1848, 1869, 1916
AjnI CCWGG 1 cut(s) 820
AjuI GAANNNNNNNTTGG 2 cut(s) 1167, 1199
AleI CACNNNNGTG 1 cut(s) 596
Alw21I GWGCWC 4 cut(s) 21, 529, 608, 754
Alw26I GTCTC 4 cut(s) 410, 505, 893, 1927
Alw44I GTGCAC 2 cut(s) 525, 604
AlwNI CAGNNNCTG 1 cut(s) 1982
Aor13HI TCCGGA 1 cut(s) 1663
AoxI GGCC 1 cut(s) 963
ApaLI GTGCAC 2 cut(s) 525, 604
ApeKI GCWGC 8 cut(s) 34, 481, 518, 659, 758, 1005, 1136, 1327
ApoI RAATTY 2 cut(s) 78, 1315
AseI ATTAAT 2 cut(s) 356, 908
AspLEI GCGC 1 cut(s) 1987
AsuC2I CCSGG 2 cut(s) 231, 1255
AsuHPI GGTGA 6 cut(s) 163, 265, 520, 883, 1619, 1705
BaeGI GKGCMC 2 cut(s) 529, 608
BalI TGGCCA 1 cut(s) 965
BanII GRGCYC 1 cut(s) 754
BarI GAAGNNNNNNTAC 2 cut(s) 1344, 1376
BbsI GAAGAC 1 cut(s) 1791
Bbv12I GWGCWC 4 cut(s) 21, 529, 608, 754
BbvCI CCTCAGC 1 cut(s) 62
BbvI GCAGC 8 cut(s) 21, 468, 505, 671, 745, 992, 1148, 1314
BccI CCATC 7 cut(s) 802, 1064, 1500, 1547, 1814, 1996, 2050
BceAI ACGGC 1 cut(s) 568
BciT130I CCWGG 1 cut(s) 822
BciVI GTATCC 1 cut(s) 1901
BclI TGATCA 1 cut(s) 1609
BcnI CCSGG 2 cut(s) 231, 1255
BcoDI GTCTC 4 cut(s) 410, 505, 893, 1927
BfaI CTAG 6 cut(s) 522, 711, 1245, 1473, 1758, 2076
BfmI CTRYAG 2 cut(s) 306, 867
BfuAI ACCTGC 1 cut(s) 523
BfuI GTATCC 1 cut(s) 1901
BglII AGATCT 2 cut(s) 836, 1525
BlpI GCTNAGC 1 cut(s) 1964
BmcAI AGTACT 1 cut(s) 865
Bme1390I CCNGG 3 cut(s) 231, 822, 1255
BmiI GGNNCC 1 cut(s) 220
BmrFI CCNGG 3 cut(s) 231, 822, 1255
BmsI GCATC 3 cut(s) 788, 1033, 2114
BpiI GAAGAC 1 cut(s) 1791
BplI GAGNNNNNCTC 2 cut(s) 1467, 1499
BpmI CTGGAG 1 cut(s) 804
Bpu10I CCTNAGC 2 cut(s) 62, 626
Bpu1102I GCTNAGC 1 cut(s) 1964
BpuEI CTTGAG 4 cut(s) 361, 411, 738, 825
BpuMI CCSGG 2 cut(s) 231, 1255
BsaBI GATNNNNATC 1 cut(s) 1848
BsaHI GRCGYC 1 cut(s) 1826
BsaI GGTCTC 2 cut(s) 505, 1927
BsaJI CCNNGG 4 cut(s) 193, 229, 262, 984
BsaWI WCCGGW 1 cut(s) 1663
BsaXI ACNNNNNCTCC 2 cut(s) 106, 136
Bsc4I CCNNNNNNNGG 4 cut(s) 445, 485, 821, 1803
Bse1I ACTGG 3 cut(s) 668, 1147, 1747
Bse3DI GCAATG 3 cut(s) 724, 1106, 1680
Bse8I GATNNNNATC 1 cut(s) 1848
BseAI TCCGGA 1 cut(s) 1663
BseBI CCWGG 1 cut(s) 822
BseDI CCNNGG 4 cut(s) 193, 229, 262, 984
BseGI GGATG 3 cut(s) 395, 948, 1825
BseJI GATNNNNATC 1 cut(s) 1848
BseLI CCNNNNNNNGG 4 cut(s) 445, 485, 821, 1803
BseMI GCAATG 3 cut(s) 724, 1106, 1680
BseMII CTCAG 5 cut(s) 76, 450, 1844, 1989, 2097
BseNI ACTGG 3 cut(s) 668, 1147, 1747
BseRI GAGGAG 7 cut(s) 146, 149, 330, 333, 414, 807, 1943
BseSI GKGCMC 2 cut(s) 529, 608
BseXI GCAGC 8 cut(s) 21, 468, 505, 671, 745, 992, 1148, 1314
BseYI CCCAGC 2 cut(s) 484, 1330
BsgI GTGCAG 4 cut(s) 620, 1095, 1292, 1400
BshFI GGCC 1 cut(s) 965
BsiHKAI GWGCWC 4 cut(s) 21, 529, 608, 754
BsiSI CCGG 3 cut(s) 231, 1254, 1664
BslFI GGGAC 4 cut(s) 76, 501, 1068, 1837
BslI CCNNNNNNNGG 4 cut(s) 445, 485, 821, 1803
BsmAI GTCTC 4 cut(s) 410, 505, 893, 1927
BsmFI GGGAC 4 cut(s) 76, 501, 1068, 1837
BsmI GAATGC 2 cut(s) 1479, 2043
BsnI GGCC 1 cut(s) 965
Bso31I GGTCTC 2 cut(s) 505, 1927
Bsp1286I GDGCHC 4 cut(s) 21, 529, 608, 754
Bsp13I TCCGGA 1 cut(s) 1663
Bsp1407I TGTACA 2 cut(s) 588, 1707
Bsp143I GATC 3 cut(s) 836, 1525, 1609
Bsp1720I GCTNAGC 1 cut(s) 1964
BspACI CCGC 6 cut(s) 469, 479, 776, 788, 1111, 1779
BspANI GGCC 1 cut(s) 965
BspCNI CTCAG 5 cut(s) 75, 451, 1845, 1988, 2096
BspEI TCCGGA 1 cut(s) 1663
BspLI GGNNCC 1 cut(s) 220
BspMI ACCTGC 1 cut(s) 523
BspQI GCTCTTC 1 cut(s) 494
BspTNI GGTCTC 2 cut(s) 505, 1927
BsrDI GCAATG 3 cut(s) 724, 1106, 1680
BsrGI TGTACA 2 cut(s) 588, 1707
BsrI ACTGG 3 cut(s) 668, 1147, 1747
BssECI CCNNGG 4 cut(s) 193, 229, 262, 984
BssMI GATC 3 cut(s) 836, 1525, 1609
BssNI GRCGYC 1 cut(s) 1826
BssT1I CCWWGG 2 cut(s) 262, 984
Bst2UI CCWGG 1 cut(s) 822
Bst4CI ACNGT 4 cut(s) 214, 241, 248, 1712
Bst6I CTCTTC 4 cut(s) 494, 1229, 1827, 2022
BstACI GRCGYC 1 cut(s) 1826
BstAUI TGTACA 2 cut(s) 588, 1707
BstC8I GCNNGC 3 cut(s) 1537, 2004, 2015
BstDEI CTNAG 8 cut(s) 62, 459, 626, 1016, 1853, 1964, 1975, 2083
BstENI CCTNNNNNAGG 1 cut(s) 819
BstF5I GGATG 3 cut(s) 395, 948, 1825
BstHHI GCGC 1 cut(s) 1987
BstKTI GATC 3 cut(s) 839, 1528, 1612
BstMAI GTCTC 4 cut(s) 410, 505, 893, 1927
BstMBI GATC 3 cut(s) 836, 1525, 1609
BstMWI GCNNNNNNNGC 6 cut(s) 342, 381, 524, 659, 758, 1999
BstNI CCWGG 1 cut(s) 822
BstNSI RCATGY 3 cut(s) 91, 379, 1660
BstSCI CCNGG 3 cut(s) 229, 820, 1253
BstSFI CTRYAG 2 cut(s) 306, 867
BstSLI GKGCMC 2 cut(s) 529, 608
BstV1I GCAGC 8 cut(s) 21, 468, 505, 671, 745, 992, 1148, 1314
BstV2I GAAGAC 1 cut(s) 1791
BstX2I RGATCY 2 cut(s) 836, 1525
BstYI RGATCY 2 cut(s) 836, 1525
BsuI GTATCC 1 cut(s) 1901
BsuRI GGCC 1 cut(s) 965
BtgZI GCGATG 3 cut(s) 282, 765, 1974
BtsCI GGATG 3 cut(s) 395, 948, 1825
BtsI GCAGTG 2 cut(s) 608, 797
BtsIMutI CAGTG 4 cut(s) 456, 608, 797, 1468
BveI ACCTGC 1 cut(s) 523
Cac8I GCNNGC 3 cut(s) 1537, 2004, 2015
CaiI CAGNNNCTG 1 cut(s) 1982
CfoI GCGC 1 cut(s) 1987
CseI GACGC 2 cut(s) 55, 1834
Csp6I GTAC 4 cut(s) 589, 864, 1708, 2122
CviAII CATG 8 cut(s) 14, 22, 88, 376, 475, 1588, 1657, 1960
CviQI GTAC 4 cut(s) 589, 864, 1708, 2122
DdeI CTNAG 8 cut(s) 62, 459, 626, 1016, 1853, 1964, 1975, 2083
DpnI GATC 3 cut(s) 838, 1527, 1611
DpnII GATC 3 cut(s) 836, 1525, 1609
DraI TTTAAA 1 cut(s) 1276
EaeI YGGCCR 1 cut(s) 963
Eam1104I CTCTTC 4 cut(s) 494, 1229, 1827, 2022
EarI CTCTTC 4 cut(s) 494, 1229, 1827, 2022
Ecl136II GAGCTC 1 cut(s) 752
Eco130I CCWWGG 2 cut(s) 262, 984
Eco24I GRGCYC 1 cut(s) 754
Eco31I GGTCTC 2 cut(s) 505, 1927
Eco53kI GAGCTC 1 cut(s) 752
Eco57I CTGAAG 3 cut(s) 516, 518, 1365
EcoICRI GAGCTC 1 cut(s) 752
EcoNI CCTNNNNNAGG 1 cut(s) 819
EcoRII CCWGG 1 cut(s) 820
EcoT14I CCWWGG 2 cut(s) 262, 984
EcoT38I GRGCYC 1 cut(s) 754
ErhI CCWWGG 2 cut(s) 262, 984
FaeI CATG 8 cut(s) 17, 25, 91, 379, 478, 1591, 1660, 1963
FalI AAGNNNNNCTT 2 cut(s) 627, 659
FaqI GGGAC 4 cut(s) 76, 501, 1068, 1837
FatI CATG 8 cut(s) 13, 21, 87, 375, 474, 1587, 1656, 1959
FbaI TGATCA 1 cut(s) 1609
FblI GTMKAC 1 cut(s) 302
FokI GGATG 3 cut(s) 382, 935, 1832
FriOI GRGCYC 1 cut(s) 754
FspBI CTAG 6 cut(s) 522, 711, 1245, 1473, 1758, 2076
GlaI GCGC 1 cut(s) 1986
GsaI CCCAGC 2 cut(s) 488, 1334
GsuI CTGGAG 1 cut(s) 804
HaeIII GGCC 1 cut(s) 965
HapII CCGG 3 cut(s) 231, 1254, 1664
HgaI GACGC 2 cut(s) 55, 1834
HhaI GCGC 1 cut(s) 1987
Hin1I GRCGYC 1 cut(s) 1826
Hin1II CATG 8 cut(s) 17, 25, 91, 379, 478, 1591, 1660, 1963
Hin6I GCGC 1 cut(s) 1985
HinP1I GCGC 1 cut(s) 1985
HindIII AAGCTT 1 cut(s) 1301
HinfI GANTC 9 cut(s) 140, 198, 321, 922, 1103, 1412, 1499, 1844, 2057
HpaII CCGG 3 cut(s) 231, 1254, 1664
HphI GGTGA 6 cut(s) 163, 265, 520, 883, 1619, 1705
Hpy166II GTNNAC 8 cut(s) 244, 303, 527, 565, 591, 606, 1195, 1708
Hpy8I GTNNAC 8 cut(s) 244, 303, 527, 565, 591, 606, 1195, 1708
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 6 cut(s) 409, 445, 540, 629, 1169, 1340
HpyCH4III ACNGT 4 cut(s) 214, 241, 248, 1712
HpyCH4IV ACGT 2 cut(s) 1542, 2051
HpyF10VI GCNNNNNNNGC 6 cut(s) 342, 381, 524, 659, 758, 1999
HpyF3I CTNAG 8 cut(s) 62, 459, 626, 1016, 1853, 1964, 1975, 2083
HpySE526I ACGT 2 cut(s) 1542, 2051
Hsp92I GRCGYC 1 cut(s) 1826
Hsp92II CATG 8 cut(s) 17, 25, 91, 379, 478, 1591, 1660, 1963
HspAI GCGC 1 cut(s) 1985
Kpn2I TCCGGA 1 cut(s) 1663
Ksp22I TGATCA 1 cut(s) 1609
Kzo9I GATC 3 cut(s) 836, 1525, 1609
LguI GCTCTTC 1 cut(s) 494
LmnI GCTCC 2 cut(s) 224, 1256
Lsp1109I GCAGC 8 cut(s) 21, 468, 505, 671, 745, 992, 1148, 1314
LweI GCATC 3 cut(s) 788, 1033, 2114
MaeI CTAG 6 cut(s) 522, 711, 1245, 1473, 1758, 2076
MaeII ACGT 2 cut(s) 1542, 2051
MaeIII GTNAC 8 cut(s) 271, 378, 829, 1652, 1738, 1970, 1978, 2047
MalI GATC 3 cut(s) 838, 1527, 1611
MboI GATC 3 cut(s) 836, 1525, 1609
MboII GAAGA 8 cut(s) 511, 797, 1246, 1364, 1762, 1796, 1844, 2039
MfeI CAATTG 3 cut(s) 900, 978, 1128
MflI RGATCY 2 cut(s) 836, 1525
MhlI GDGCHC 4 cut(s) 21, 529, 608, 754
MlsI TGGCCA 1 cut(s) 965
MluCI AATT 9 cut(s) 78, 900, 978, 1089, 1128, 1315, 1685, 2109, 2117
MluNI TGGCCA 1 cut(s) 965
MlyI GAGTC 5 cut(s) 149, 330, 916, 1112, 1406
MmeI TCCRAC 4 cut(s) 201, 1413, 1621, 1821
Mox20I TGGCCA 1 cut(s) 965
MroI TCCGGA 1 cut(s) 1663
MscI TGGCCA 1 cut(s) 965
MseI TTAA 9 cut(s) 356, 908, 914, 1158, 1275, 1509, 1530, 1724, 2097
MslI CAYNNNNRTG 2 cut(s) 358, 596
Msp20I TGGCCA 1 cut(s) 965
MspA1I CMGCKG 3 cut(s) 481, 776, 1779
MspI CCGG 3 cut(s) 231, 1254, 1664
MspR9I CCNGG 3 cut(s) 231, 822, 1255
MunI CAATTG 3 cut(s) 900, 978, 1128
Mva1269I GAATGC 2 cut(s) 1479, 2043
MvaI CCWGG 1 cut(s) 822
MwoI GCNNNNNNNGC 6 cut(s) 342, 381, 524, 659, 758, 1999
NciI CCSGG 2 cut(s) 231, 1255
NdeII GATC 3 cut(s) 836, 1525, 1609
NlaIII CATG 8 cut(s) 17, 25, 91, 379, 478, 1591, 1660, 1963
NlaIV GGNNCC 1 cut(s) 220
NmeAIII GCCGAG 1 cut(s) 1483
NmuCI GTSAC 1 cut(s) 271
NspI RCATGY 3 cut(s) 91, 379, 1660
OliI CACNNNNGTG 1 cut(s) 596
PciI ACATGT 1 cut(s) 87
PciSI GCTCTTC 1 cut(s) 494
PctI GAATGC 2 cut(s) 1479, 2043
PfeI GAWTC 4 cut(s) 198, 1499, 1844, 2057
PleI GAGTC 5 cut(s) 148, 329, 916, 1111, 1406
PpsI GAGTC 5 cut(s) 148, 329, 916, 1111, 1406
PscI ACATGT 1 cut(s) 87
PshBI ATTAAT 2 cut(s) 356, 908
Psp124BI GAGCTC 1 cut(s) 754
Psp6I CCWGG 1 cut(s) 820
PspFI CCCAGC 2 cut(s) 484, 1330
PspGI CCWGG 1 cut(s) 820
PspN4I GGNNCC 1 cut(s) 220
PstNI CAGNNNCTG 1 cut(s) 1982
PsuI RGATCY 2 cut(s) 836, 1525
RsaI GTAC 4 cut(s) 590, 865, 1709, 2123
RsaNI GTAC 4 cut(s) 589, 864, 1708, 2122
RseI CAYNNNNRTG 2 cut(s) 358, 596
SacI GAGCTC 1 cut(s) 754
SapI GCTCTTC 1 cut(s) 494
SaqAI TTAA 9 cut(s) 356, 908, 914, 1158, 1275, 1509, 1530, 1724, 2097
Sau3AI GATC 3 cut(s) 836, 1525, 1609
ScaI AGTACT 1 cut(s) 865
SchI GAGTC 5 cut(s) 149, 330, 916, 1112, 1406
ScrFI CCNGG 3 cut(s) 231, 822, 1255
SduI GDGCHC 4 cut(s) 21, 529, 608, 754
SfaNI GCATC 3 cut(s) 788, 1033, 2114
SfcI CTRYAG 2 cut(s) 306, 867
SmiMI CAYNNNNRTG 2 cut(s) 358, 596
SmlI CTYRAG 4 cut(s) 340, 426, 753, 840
SmoI CTYRAG 4 cut(s) 340, 426, 753, 840
Sse9I AATT 9 cut(s) 78, 900, 978, 1089, 1128, 1315, 1685, 2109, 2117
SsiI CCGC 6 cut(s) 469, 479, 776, 788, 1111, 1779
SspI AATATT 2 cut(s) 918, 1599
SspMI CTAG 6 cut(s) 522, 711, 1245, 1473, 1758, 2076
SstI GAGCTC 1 cut(s) 754
StyD4I CCNGG 3 cut(s) 229, 820, 1253
StyI CCWWGG 2 cut(s) 262, 984
TaaI ACNGT 4 cut(s) 214, 241, 248, 1712
TaiI ACGT 2 cut(s) 1545, 2054
TaqI TCGA 5 cut(s) 69, 620, 724, 1410, 1522
TasI AATT 9 cut(s) 78, 900, 978, 1089, 1128, 1315, 1685, 2109, 2117
TatI WGTACW 3 cut(s) 588, 863, 1707
TauI GCSGC 3 cut(s) 481, 779, 1782
TfiI GAWTC 4 cut(s) 198, 1499, 1844, 2057
Tru1I TTAA 9 cut(s) 356, 908, 914, 1158, 1275, 1509, 1530, 1724, 2097
Tru9I TTAA 9 cut(s) 356, 908, 914, 1158, 1275, 1509, 1530, 1724, 2097
TscAI CASTG 4 cut(s) 463, 608, 804, 1468
TseFI GTSAC 1 cut(s) 271
TseI GCWGC 8 cut(s) 34, 481, 518, 659, 758, 1005, 1136, 1327
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 9 cut(s) 240, 463, 744, 866, 1101, 1386, 1577, 1890, 2115
TspGWI ACGGA 2 cut(s) 31, 1695
TspRI CASTG 4 cut(s) 463, 608, 804, 1468
VneI GTGCAC 2 cut(s) 525, 604
VspI ATTAAT 2 cut(s) 356, 908
XagI CCTNNNNNAGG 1 cut(s) 819
XapI RAATTY 2 cut(s) 78, 1315
XbaI TCTAGA 1 cut(s) 1244
XceI RCATGY 3 cut(s) 91, 379, 1660
XmiI GTMKAC 1 cut(s) 302
XspI CTAG 6 cut(s) 522, 711, 1245, 1473, 1758, 2076
ZrmI AGTACT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.