MD00G1102200.v1.1

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000283
Physical Location & Seq
Reverse (-)
21548381 .. 21551190
2810 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1102200.v1.1.491

Sequence Viewer

Length: 2046 bp
ATGTCTTGCTACCACACCTGCATTGCTCTTGTTAATACAAAAACATATGTTATGATGAATCACCAAAAATTGAAAAGCATGACTACCCTTCTTTTGATTTTCTCCATTTTTACTCTGCCCAGCTTTCTGACCATTACGGAAGCAGTTTATCTCTACCACGTCTGCTCAAACACCACCACCTTCACCCCAAACTCCACCTTCCAGTCCAACGTCAACCGCCTCCTCTCCACCCTCTCCTCCAACTCCAACCGCTCCACCGGCTTCTACAACGCCACCGTCCAAACCCCAAACAACGCCGTCTACGGCCTCTTCCTCTGCCGCGGCGACGTCGTGGGCACCGACGCTTGCGAAACCTGTGTCGCCACCGTAACCGCCGAGGCCCCAGAACGCTGCCCCATAGAGAAACAGGTGGTGATCTGGTACGACGACTGCATGCTACGCTACTCCGACGAGTCATTCTTTTCCACCTCGGCCGAATCGCCTAGCTTGTACATGTGGAACACGCAGAATGCAAGTGGTATAACGGACCAAAACCTGTTCAACAAGGTACTGGCAGCTACGGTCACCGAGGTGGCCGCTCAGACTTCCAATAGCAGTCACATGTTTGCGACGAAAGAAACGAGTGTTTCAGGTTTCATTACGCTGTACGGATTTGGGCAGTGCACGCAGGATCTGTTGTCTGGAGATTGCAATTGGTGTCTTCTGGAAGCTGCAACACAAACTCCTAGAAGCAATGCTGGGGGACGAGTTCTAAAACCCAGTTGCAATCTTAGGTTTGAAATTTACCCCTTCTACTCCTCCGCCCCACCGCCGCCAGCCTCCATCAGAGAAGGGCCTAAAGTGTCGCCAGCAAATATTCCACCTCCGGTTCCAGAAGCAAAGAAGAGTAACAGAAAAGTAATTATCATCATCGTGGTGGTCGTGGTTTTGGTTGCTTTTGTTACCATTCTCAGCAGCATATGCATTTACTCGAGAGTCAAGAAACGAGGGTTACAACTTGAAGTTGAAGCTAAGGATGATACAAAGACTGTTGAGTCCTTGCAATTCGACTTGGGAACCATTGAAACCGCCACAAACAAGTTTTCTGATAATAACAAGTTAGGTGAAGGCGGATTTGGTGTAGTTTTCAAGGGAACACTTGCTAATGAACAAGAAATTGCAGTGAAGAGGTTGTCAAAAAGCTCTAGACAAGGCGTCCAAGAATTTAAGAACGAGGTTGCATTGGTAGCCAAACTTCAACACAGAAATCTTGTTAGGCTCCTGGGATTTTGTTTGGAAGGAGAGGAAACCCTACTTGTTTATGAATATGTGCGAAACAAAAGTCTTGATTATTTTCTTTTTGAATCCAGGAAACGTGAACAGCTGGATTGGTCAAGCCGTTGCATGATAATAGGAGGAATCACTCGAGGAATTTTATATCTTCATGAAGATTCAAGGCATAGAGTTATACATCGTGATCTAAAAGCGAGTAACATCCTGTTAGATGAAAATATGAATCCAAAAATATCAGATTTTGGTATGGCAAGAATGTTTGGAGTGGATGATCAAACTCAAGGAAACACCAAAAGAATTGTCGGCACCTATGGTTACATGGCTCCAGAGTATGCTATGGAGGGATTGTATTCAATAAAATCTGATGTATATAGCTTCGGAGTACTTTTGCTTGAGATCACAACGGGGAGAAAGAACTTTTTGGGTTTTCATCCGACAAATGGTGCACCGACTCTTATAGGCTACGCTTGGAAATTATGGAATGAAGGGAAAGTGTTGGAGTTGATGGATCCGTTGTTGAAAGGCTCATGCAGTCCAAATGAATTCCTGAGATACGTTCATATCGGATTATTGTGTGTTCAAGAAGATGCAAACAACAGGCCGACCATGTCATCAGTTGTTCTTATGTTGAAAAGTGAAACCATTAGTCTTTCTAAACCTGAGCGACCAGCCTTCTTTACAGGGAGATTTATTGATCATCGTGATCATGTAGCAGTCAGTGCTCACAATTGCTCGGCCAATGGCTTGACGATTTCTAATGTTGTGGCTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3403 Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

682

Amino Acids

75.76

Weight (kDa)

8.0

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 55 - 150 2.3e-26 Salt stress response/antifungal
Stress-antifung PF01657 172 - 261 6.7e-08 Salt stress response/antifungal
Pkinase PF00069 362 - 565 5.1e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 364 - 631 5.9e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000283)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45860 AT4G05200 AT4G05200 AT4G11521 AT4G11530 AT4G23130 AT4G23130 AT4G23130 AT4G23140 AT4G23140 AT4G23150 AT4G23160 AT4G23170 AT4G23180 AT4G23180 AT4G23180 AT4G23230 AT4G23270 AT4G23270 AT4G23270 AT4G23280 AT4G23280 AT4G23280 AT4G23310 AT4G23310
fragaria_vesca FvH4_3g02772 FvH4_3g02772 FvH4_3g02773 FvH4_3g02773 FvH4_3g02774 FvH4_3g02800 FvH4_3g02820 FvH4_3g02820 FvH4_3g02830
malus_domestica MD00G1101900.v1.1 MD00G1102100.v1.1 MD00G1102200.v1.1 MD01G1098200.v1.1 MD05G1340000.v1.1 MD05G1340300.v1.1 MD07G1014100.v1.1 MD07G1014300.v1.1 MD07G1014400.v1.1 MD07G1014700.v1.1 MD10G1312900.v1.1 MD11G1022600.v1.1 MD11G1291500.v1.1 MD14G1207100.v1.1 MD16G1096600.v1.1
prunus_persica Prupe.4G027300_v2.0.a1 Prupe.4G027400_v2.0.a1 Prupe.4G027700_v2.0.a1 Prupe.4G027800_v2.0.a1
pyrus_communis pycom05g30960 pycom10g00940 pycom10g00950 pycom10g26430 pycom10g26450 pycom10g26460 pycom10g26470 pycom10g26480 pycom10g26490 pycom10g26500 pycom10g26510 pycom10g26570 pycom11g01820 pycom11g04000 pycom14g17130 pycom14g17140 pycom15g28820
rosa_chinensis RchiOBHm_Chr4g0408141 RchiOBHm_Chr5g0004211 RchiOBHm_Chr5g0004231 RchiOBHm_Chr5g0004241 RchiOBHm_Chr5g0004261
rosa_laevigata RLG00000008668 RLG00000008672 RLG00000031212 RLG00000031217
rosa_multiflora Rmu_co8162658.1_g000001 Rmu_co8336297.1_g000001 Rmu_sc0002073.1_g000001 Rmu_sc0004964.1_g000002 Rmu_sc0004964.1_g000003 Rmu_sc0004964.1_g000007 Rmu_sc0006006.1_g000007 Rmu_sc0007868.1_g000015 Rmu_sc0007868.1_g000016 Rmu_sc0007868.1_g000017 Rmu_sc0007868.1_g000021 Rmu_sc0007868.1_g000023 Rmu_sc0019654.1_g000002 Rmu_sc0038540.1_g000001
rosa_roxburghii Rroxscaffold_1G00071450 Rroxscaffold_1G00071460 Rroxscaffold_1G00071470 Rroxscaffold_1G00071560 Rroxscaffold_1G00071720 Rroxscaffold_1G00071770 Rroxscaffold_5G00352640
rosa_rugosa Rorug04G0083700 Rorug04G0410700 Rorug04G0410800 Rorug04G0410900
rosa_samantha Rh4BG145400 Rh4CG154900 Rh4DG141900 Rh5BG037500 Rh5BG037600 Rh5BG037700 Rh5CG041000 Rh5CG041100 Rh5CG041200 Rh5CG041300 Rh5CG041400 Rh5DG037100
rosa_wichuraiana Rw4G012130 Rw5G003450 Rw5G003460 Rw5G003470 Rw5G003480 Rw5G003640 Rw5G003660 Rw5G003670 Rw5G003680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 26
AasI GACNNNNNNGTC 1 cut(s) 1033
AatII GACGTC 1 cut(s) 330
Acc36I ACCTGC 1 cut(s) 26
AccB1I GGYRCC 2 cut(s) 335, 1577
AccBSI CCGCTC 2 cut(s) 252, 578
AccI GTMKAC 1 cut(s) 300
AccII CGCG 1 cut(s) 321
AclWI GGATC 3 cut(s) 678, 1775, 1788
AcoI YGGCCR 3 cut(s) 471, 573, 2007
AcsI RAATTY 4 cut(s) 780, 1202, 1410, 1814
AcyI GRCGYC 2 cut(s) 327, 1194
AfaI GTAC 5 cut(s) 422, 491, 549, 647, 1656
AfiI CCNNNNNNNGG 1 cut(s) 1712
AflIII ACRYGT 2 cut(s) 492, 600
AhdI GACNNNNNGTC 1 cut(s) 1193
AjiI CACGTC 1 cut(s) 160
AjnI CCWGG 2 cut(s) 1260, 1346
AjuI GAANNNNNNNTTGG 2 cut(s) 1098, 1130
AleI CACNNNNGTG 1 cut(s) 569
AluBI AGCT 8 cut(s) 123, 486, 557, 710, 1010, 1182, 1363, 1647
AluI AGCT 8 cut(s) 123, 486, 557, 710, 1010, 1182, 1363, 1647
Alw21I GWGCWC 3 cut(s) 665, 1720, 1996
Alw44I GTGCAC 2 cut(s) 661, 1716
AlwI GGATC 3 cut(s) 678, 1775, 1788
AlwNI CAGNNNCTG 1 cut(s) 673
Ama87I CYCGRG 2 cut(s) 970, 1404
AoxI GGCC 7 cut(s) 304, 378, 471, 573, 833, 1871, 2007
ApaLI GTGCAC 2 cut(s) 661, 1716
ApeKI GCWGC 4 cut(s) 390, 554, 710, 954
ApoI RAATTY 4 cut(s) 780, 1202, 1410, 1814
ArsI GACNNNNNNTTYG 6 cut(s) 342, 374, 1306, 1338, 1557, 1589
AspS9I GGNCC 3 cut(s) 379, 526, 833
AsuHPI GGTGA 5 cut(s) 53, 175, 424, 556, 1115
AvaI CYCGRG 2 cut(s) 970, 1404
AvaII GGWCC 1 cut(s) 526
BaeGI GKGCMC 3 cut(s) 338, 665, 1720
BamHI GGATCC 1 cut(s) 1780
BanI GGYRCC 2 cut(s) 335, 1577
BarI GAAGNNNNNNTAC 2 cut(s) 1218, 1250
BbsI GAAGAC 1 cut(s) 692
Bbv12I GWGCWC 3 cut(s) 665, 1720, 1996
BbvI GCAGC 4 cut(s) 377, 566, 697, 966
BccI CCATC 2 cut(s) 830, 1771
BceAI ACGGC 3 cut(s) 281, 319, 1362
BciT130I CCWGG 2 cut(s) 1262, 1348
BclI TGATCA 3 cut(s) 1543, 1966, 1975
BfaI CTAG 3 cut(s) 483, 726, 1185
BfuAI ACCTGC 1 cut(s) 26
BisI GCNGC 8 cut(s) 319, 322, 391, 555, 576, 711, 812, 955
BlsI GCNGC 8 cut(s) 320, 323, 392, 556, 577, 712, 813, 956
BmcAI AGTACT 1 cut(s) 1656
Bme1390I CCNGG 2 cut(s) 1262, 1348
Bme18I GGWCC 1 cut(s) 526
BmeRI GACNNNNNGTC 1 cut(s) 1193
BmeT110I CYCGRG 2 cut(s) 970, 1404
BmgBI CACGTC 1 cut(s) 160
BmgT120I GGNCC 3 cut(s) 379, 526, 833
BmiI GGNNCC 8 cut(s) 337, 381, 870, 1057, 1259, 1579, 1596, 1782
BmrFI CCNGG 2 cut(s) 1262, 1348
BmrI ACTGGG 1 cut(s) 753
BmsI GCATC 1 cut(s) 1849
BmuI ACTGGG 1 cut(s) 753
BpiI GAAGAC 1 cut(s) 692
BpmI CTGGAG 2 cut(s) 702, 1581
Bpu10I CCTNAGC 2 cut(s) 1011, 1932
BpuEI CTTGAG 2 cut(s) 1536, 1685
BsaHI GRCGYC 2 cut(s) 327, 1194
BsaJI CCNNGG 5 cut(s) 319, 375, 468, 567, 1261
BsaWI WCCGGW 1 cut(s) 865
BsaXI ACNNNNNCTCC 4 cut(s) 706, 736, 1604, 1634
Bsc4I CCNNNNNNNGG 1 cut(s) 1712
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 3 cut(s) 202, 555, 759
Bse3DI GCAATG 2 cut(s) 21, 739
BseBI CCWGG 2 cut(s) 1262, 1348
BseDI CCNNGG 5 cut(s) 319, 375, 468, 567, 1261
BseGI GGATG 4 cut(s) 1021, 1473, 1546, 1702
BseLI CCNNNNNNNGG 1 cut(s) 1712
BseMI GCAATG 2 cut(s) 21, 739
BseMII CTCAG 4 cut(s) 593, 964, 1811, 1923
BseNI ACTGG 3 cut(s) 202, 555, 759
BseRI GAGGAG 3 cut(s) 212, 226, 787
BseSI GKGCMC 3 cut(s) 338, 665, 1720
BseX3I CGGCCG 1 cut(s) 471
BseXI GCAGC 4 cut(s) 377, 566, 697, 966
BseYI CCCAGC 2 cut(s) 119, 737
Bsh1236I CGCG 1 cut(s) 321
Bsh1285I CGRYCG 1 cut(s) 474
BshFI GGCC 7 cut(s) 306, 380, 473, 575, 835, 1873, 2009
BshNI GGYRCC 2 cut(s) 335, 1577
BsiEI CGRYCG 1 cut(s) 474
BsiHKAI GWGCWC 3 cut(s) 665, 1720, 1996
BsiHKCI CYCGRG 2 cut(s) 970, 1404
BsiSI CCGG 2 cut(s) 258, 866
BslFI GGGAC 1 cut(s) 756
BslI CCNNNNNNNGG 1 cut(s) 1712
BsmFI GGGAC 1 cut(s) 756
BsmI GAATGC 1 cut(s) 514
BsnI GGCC 7 cut(s) 306, 380, 473, 575, 835, 1873, 2009
BsoBI CYCGRG 2 cut(s) 970, 1404
Bsp1286I GDGCHC 4 cut(s) 338, 665, 1720, 1996
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 8 cut(s) 414, 670, 1456, 1543, 1668, 1780, 1966, 1975
BspANI GGCC 7 cut(s) 306, 380, 473, 575, 835, 1873, 2009
BspCNI CTCAG 4 cut(s) 592, 963, 1812, 1924
BspFNI CGCG 1 cut(s) 321
BspHI TCATGA 1 cut(s) 1423
BspLI GGNNCC 8 cut(s) 337, 381, 870, 1057, 1259, 1579, 1596, 1782
BspMI ACCTGC 1 cut(s) 26
BspPI GGATC 3 cut(s) 678, 1775, 1788
BspT107I GGYRCC 2 cut(s) 335, 1577
BsrBI CCGCTC 2 cut(s) 252, 578
BsrDI GCAATG 2 cut(s) 21, 739
BsrFI RCCGGY 1 cut(s) 257
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 3 cut(s) 202, 555, 759
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 5 cut(s) 319, 375, 468, 567, 1261
BssMI GATC 8 cut(s) 414, 670, 1456, 1543, 1668, 1780, 1966, 1975
BssNI GRCGYC 2 cut(s) 327, 1194
Bst2UI CCWGG 2 cut(s) 1262, 1348
Bst4CI ACNGT 4 cut(s) 277, 367, 562, 1030
Bst6I CTCTTC 3 cut(s) 314, 878, 1160
BstACI GRCGYC 2 cut(s) 327, 1194
BstAPI GCANNNNNTGC 2 cut(s) 960, 1991
BstAUI TGTACA 1 cut(s) 489
BstC8I GCNNGC 5 cut(s) 346, 434, 665, 816, 849
BstDEI CTNAG 6 cut(s) 579, 770, 950, 1011, 1820, 1932
BstDSI CCRYGG 1 cut(s) 319
BstEII GGTNACC 1 cut(s) 562
BstF5I GGATG 4 cut(s) 1021, 1473, 1546, 1702
BstFNI CGCG 1 cut(s) 321
BstKTI GATC 8 cut(s) 417, 673, 1459, 1546, 1671, 1783, 1969, 1978
BstMBI GATC 8 cut(s) 414, 670, 1456, 1543, 1668, 1780, 1966, 1975
BstMCI CGRYCG 1 cut(s) 474
BstMWI GCNNNNNNNGC 6 cut(s) 258, 438, 664, 960, 1226, 1991
BstNI CCWGG 2 cut(s) 1262, 1348
BstNSI RCATGY 3 cut(s) 436, 496, 604
BstPI GGTNACC 1 cut(s) 562
BstSCI CCNGG 2 cut(s) 1260, 1346
BstSLI GKGCMC 3 cut(s) 338, 665, 1720
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 4 cut(s) 377, 566, 697, 966
BstV2I GAAGAC 1 cut(s) 692
BstX2I RGATCY 2 cut(s) 670, 1780
BstYI RGATCY 2 cut(s) 670, 1780
BstZI CGGCCG 1 cut(s) 471
BsuRI GGCC 7 cut(s) 306, 380, 473, 575, 835, 1873, 2009
BtgI CCRYGG 1 cut(s) 319
BtrI CACGTC 1 cut(s) 160
BtsCI GGATG 4 cut(s) 1021, 1473, 1546, 1702
BtsI GCAGTG 2 cut(s) 665, 1167
BtsIMutI CAGTG 3 cut(s) 665, 1167, 1996
BveI ACCTGC 1 cut(s) 26
Cac8I GCNNGC 5 cut(s) 346, 434, 665, 816, 849
CaiI CAGNNNCTG 1 cut(s) 673
CciI TCATGA 1 cut(s) 1423
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 3 cut(s) 379, 526, 833
Cfr42I CCGCGG 1 cut(s) 322
CseI GACGC 2 cut(s) 350, 1183
Csp6I GTAC 5 cut(s) 421, 490, 548, 646, 1655
CviQI GTAC 5 cut(s) 421, 490, 548, 646, 1655
DdeI CTNAG 6 cut(s) 579, 770, 950, 1011, 1820, 1932
DpnI GATC 8 cut(s) 416, 672, 1458, 1545, 1670, 1782, 1968, 1977
DpnII GATC 8 cut(s) 414, 670, 1456, 1543, 1668, 1780, 1966, 1975
DrdI GACNNNNNNGTC 1 cut(s) 1033
DriI GACNNNNNGTC 1 cut(s) 1193
DseDI GACNNNNNNGTC 1 cut(s) 1033
EaeI YGGCCR 3 cut(s) 471, 573, 2007
EagI CGGCCG 1 cut(s) 471
Eam1104I CTCTTC 3 cut(s) 314, 878, 1160
Eam1105I GACNNNNNGTC 1 cut(s) 1193
EarI CTCTTC 3 cut(s) 314, 878, 1160
EciI GGCGGA 2 cut(s) 790, 1125
EclXI CGGCCG 1 cut(s) 471
Eco47I GGWCC 1 cut(s) 526
Eco52I CGGCCG 1 cut(s) 471
Eco88I CYCGRG 2 cut(s) 970, 1404
Eco91I GGTNACC 1 cut(s) 562
EcoO109I RGGNCCY 2 cut(s) 379, 833
EcoO65I GGTNACC 1 cut(s) 562
EcoRI GAATTC 1 cut(s) 1814
EcoRII CCWGG 2 cut(s) 1260, 1346
EcoT22I ATGCAT 1 cut(s) 965
FaqI GGGAC 1 cut(s) 756
FauNDI CATATG 2 cut(s) 46, 959
FbaI TGATCA 3 cut(s) 1543, 1966, 1975
FblI GTMKAC 1 cut(s) 300
Fnu4HI GCNGC 8 cut(s) 319, 322, 391, 555, 576, 711, 812, 955
FokI GGATG 4 cut(s) 1028, 1460, 1553, 1689
Fsp4HI GCNGC 8 cut(s) 319, 322, 391, 555, 576, 711, 812, 955
FspBI CTAG 3 cut(s) 483, 726, 1185
GluI GCNGC 8 cut(s) 319, 322, 391, 555, 576, 711, 812, 955
GsaI CCCAGC 2 cut(s) 123, 741
GsuI CTGGAG 2 cut(s) 702, 1581
HaeIII GGCC 7 cut(s) 306, 380, 473, 575, 835, 1873, 2009
HapII CCGG 2 cut(s) 258, 866
HgaI GACGC 2 cut(s) 350, 1183
Hin1I GRCGYC 2 cut(s) 327, 1194
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HpaII CCGG 2 cut(s) 258, 866
HphI GGTGA 5 cut(s) 53, 175, 424, 556, 1115
Hpy166II GTNNAC 5 cut(s) 214, 301, 663, 1358, 1718
Hpy8I GTNNAC 5 cut(s) 214, 301, 663, 1358, 1718
Hpy99I CGWCG 6 cut(s) 329, 332, 344, 428, 452, 613
HpyAV CCTTC 9 cut(s) 98, 190, 208, 799, 824, 1100, 1271, 1751, 1954
HpyCH4III ACNGT 4 cut(s) 277, 367, 562, 1030
HpyCH4IV ACGT 5 cut(s) 159, 210, 327, 1354, 1827
HpyF10VI GCNNNNNNNGC 6 cut(s) 258, 438, 664, 960, 1226, 1991
HpyF3I CTNAG 6 cut(s) 579, 770, 950, 1011, 1820, 1932
HpySE526I ACGT 5 cut(s) 159, 210, 327, 1354, 1827
Hsp92I GRCGYC 2 cut(s) 327, 1194
Ksp22I TGATCA 3 cut(s) 1543, 1966, 1975
KspI CCGCGG 1 cut(s) 322
Kzo9I GATC 8 cut(s) 414, 670, 1456, 1543, 1668, 1780, 1966, 1975
LmnI GCTCC 3 cut(s) 257, 1263, 1600
Lsp1109I GCAGC 4 cut(s) 377, 566, 697, 966
LweI GCATC 1 cut(s) 1849
MaeI CTAG 3 cut(s) 483, 726, 1185
MaeII ACGT 5 cut(s) 159, 210, 327, 1354, 1827
MaeIII GTNAC 8 cut(s) 367, 562, 596, 887, 940, 990, 1469, 1586
MalI GATC 8 cut(s) 416, 672, 1458, 1545, 1670, 1782, 1968, 1977
MbiI CCGCTC 2 cut(s) 252, 578
MboI GATC 8 cut(s) 414, 670, 1456, 1543, 1668, 1780, 1966, 1975
MboII GAAGA 7 cut(s) 301, 692, 895, 1177, 1412, 1439, 1868
MfeI CAATTG 2 cut(s) 691, 1999
MflI RGATCY 2 cut(s) 670, 1780
MhlI GDGCHC 4 cut(s) 338, 665, 1720, 1996
MlyI GAGTC 4 cut(s) 461, 984, 1043, 1717
MmeI TCCRAC 6 cut(s) 231, 264, 270, 471, 1730, 1749
Mph1103I ATGCAT 1 cut(s) 965
MseI TTAA 2 cut(s) 33, 1206
MslI CAYNNNNRTG 3 cut(s) 569, 911, 914
MspA1I CMGCKG 2 cut(s) 321, 1363
MspI CCGG 2 cut(s) 258, 866
MspR9I CCNGG 2 cut(s) 1262, 1348
MunI CAATTG 2 cut(s) 691, 1999
Mva1269I GAATGC 1 cut(s) 514
MvaI CCWGG 2 cut(s) 1262, 1348
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 6 cut(s) 258, 438, 664, 960, 1226, 1991
NdeI CATATG 2 cut(s) 46, 959
NdeII GATC 8 cut(s) 414, 670, 1456, 1543, 1668, 1780, 1966, 1975
NlaIV GGNNCC 8 cut(s) 337, 381, 870, 1057, 1259, 1579, 1596, 1782
NmeAIII GCCGAG 3 cut(s) 400, 449, 1985
NmuCI GTSAC 2 cut(s) 562, 596
NsiI ATGCAT 1 cut(s) 965
NspI RCATGY 3 cut(s) 436, 496, 604
OliI CACNNNNGTG 1 cut(s) 569
PaeI GCATGC 1 cut(s) 436
PaeR7I CTCGAG 2 cut(s) 970, 1404
PagI TCATGA 1 cut(s) 1423
PaqCI CACCTGC 1 cut(s) 26
PciI ACATGT 2 cut(s) 492, 600
PcsI WCGNNNNNNNCGW 3 cut(s) 336, 617, 918
PctI GAATGC 1 cut(s) 514
PfeI GAWTC 6 cut(s) 58, 476, 1343, 1398, 1430, 1495
PflFI GACNNNGTC 1 cut(s) 1879
PfoI TCCNGGA 1 cut(s) 1346
PkrI GCNGC 8 cut(s) 320, 323, 392, 556, 577, 712, 813, 956
PleI GAGTC 4 cut(s) 460, 983, 1042, 1717
PpsI GAGTC 4 cut(s) 460, 983, 1042, 1717
PscI ACATGT 2 cut(s) 492, 600
Psp6I CCWGG 2 cut(s) 1260, 1346
PspEI GGTNACC 1 cut(s) 562
PspFI CCCAGC 2 cut(s) 119, 737
PspGI CCWGG 2 cut(s) 1260, 1346
PspN4I GGNNCC 8 cut(s) 337, 381, 870, 1057, 1259, 1579, 1596, 1782
PspPI GGNCC 3 cut(s) 379, 526, 833
PspXI VCTCGAGB 1 cut(s) 1404
PstNI CAGNNNCTG 1 cut(s) 673
PsuI RGATCY 2 cut(s) 670, 1780
PsyI GACNNNGTC 1 cut(s) 1879
PvuII CAGCTG 1 cut(s) 1363
RsaI GTAC 5 cut(s) 422, 491, 549, 647, 1656
RsaNI GTAC 5 cut(s) 421, 490, 548, 646, 1655
RseI CAYNNNNRTG 3 cut(s) 569, 911, 914
SacII CCGCGG 1 cut(s) 322
SaqAI TTAA 2 cut(s) 33, 1206
SatI GCNGC 8 cut(s) 319, 322, 391, 555, 576, 711, 812, 955
Sau3AI GATC 8 cut(s) 414, 670, 1456, 1543, 1668, 1780, 1966, 1975
Sau96I GGNCC 3 cut(s) 379, 526, 833
ScaI AGTACT 1 cut(s) 1656
SchI GAGTC 4 cut(s) 461, 984, 1043, 1717
ScrFI CCNGG 2 cut(s) 1262, 1348
SduI GDGCHC 4 cut(s) 338, 665, 1720, 1996
SfaNI GCATC 1 cut(s) 1849
Sfr274I CTCGAG 2 cut(s) 970, 1404
Sfr303I CCGCGG 1 cut(s) 322
SgrBI CCGCGG 1 cut(s) 322
SinI GGWCC 1 cut(s) 526
SlaI CTCGAG 2 cut(s) 970, 1404
SmiMI CAYNNNNRTG 3 cut(s) 569, 911, 914
SmlI CTYRAG 4 cut(s) 970, 1404, 1551, 1664
SmoI CTYRAG 4 cut(s) 970, 1404, 1551, 1664
SphI GCATGC 1 cut(s) 436
SspI AATATT 1 cut(s) 856
SspMI CTAG 3 cut(s) 483, 726, 1185
StyD4I CCNGG 2 cut(s) 1260, 1346
TaaI ACNGT 4 cut(s) 277, 367, 562, 1030
TaiI ACGT 5 cut(s) 162, 213, 330, 1357, 1830
TaqI TCGA 3 cut(s) 971, 1047, 1405
TatI WGTACW 2 cut(s) 489, 1654
TauI GCSGC 4 cut(s) 321, 324, 578, 814
TfiI GAWTC 6 cut(s) 58, 476, 1343, 1398, 1430, 1495
Tru1I TTAA 2 cut(s) 33, 1206
Tru9I TTAA 2 cut(s) 33, 1206
TscAI CASTG 3 cut(s) 665, 1167, 1996
TseFI GTSAC 2 cut(s) 562, 596
TseI GCWGC 4 cut(s) 390, 554, 710, 954
Tsp45I GTSAC 2 cut(s) 562, 596
TspGWI ACGGA 4 cut(s) 152, 539, 663, 1773
TspRI CASTG 3 cut(s) 665, 1167, 1996
Tth111I GACNNNGTC 1 cut(s) 1879
VneI GTGCAC 2 cut(s) 661, 1716
VpaK11BI GGWCC 1 cut(s) 526
XapI RAATTY 4 cut(s) 780, 1202, 1410, 1814
XbaI TCTAGA 1 cut(s) 1184
XceI RCATGY 3 cut(s) 436, 496, 604
XhoI CTCGAG 2 cut(s) 970, 1404
XmiI GTMKAC 1 cut(s) 300
XspI CTAG 3 cut(s) 483, 726, 1185
ZraI GACGTC 1 cut(s) 328
ZrmI AGTACT 1 cut(s) 1656
Zsp2I ATGCAT 1 cut(s) 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.