Serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 482 |
| AccBSI | CCGCTC | 1 cut(s) | 304 |
| AccI | GTMKAC | 1 cut(s) | 228 |
| AciI | CCGC | 1 cut(s) | 302 |
| AclI | AACGTT | 1 cut(s) | 643 |
| AcoI | YGGCCR | 1 cut(s) | 448 |
| AcsI | RAATTY | 2 cut(s) | 416, 768 |
| AcuI | CTGAAG | 1 cut(s) | 482 |
| AfaI | GTAC | 1 cut(s) | 31 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 705 |
| AgsI | TTSAA | 4 cut(s) | 126, 250, 310, 438 |
| AhlI | ACTAGT | 1 cut(s) | 32 |
| AjnI | CCWGG | 3 cut(s) | 160, 291, 698 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 360, 392 |
| AluBI | AGCT | 7 cut(s) | 138, 145, 188, 298, 326, 361, 800 |
| AluI | AGCT | 7 cut(s) | 138, 145, 188, 298, 326, 361, 800 |
| Alw21I | GWGCWC | 1 cut(s) | 300 |
| Alw26I | GTCTC | 2 cut(s) | 248, 680 |
| Ama87I | CYCGRG | 1 cut(s) | 669 |
| AoxI | GGCC | 2 cut(s) | 448, 635 |
| ApeKI | GCWGC | 2 cut(s) | 111, 188 |
| ApoI | RAATTY | 2 cut(s) | 416, 768 |
| AspS9I | GGNCC | 3 cut(s) | 240, 289, 380 |
| AsuHPI | GGTGA | 1 cut(s) | 17 |
| AvaI | CYCGRG | 1 cut(s) | 669 |
| AvaII | GGWCC | 3 cut(s) | 240, 289, 380 |
| BaeGI | GKGCMC | 1 cut(s) | 280 |
| BalI | TGGCCA | 1 cut(s) | 450 |
| BanII | GRGCYC | 1 cut(s) | 300 |
| Bbv12I | GWGCWC | 1 cut(s) | 300 |
| BbvI | GCAGC | 2 cut(s) | 123, 175 |
| BccI | CCATC | 2 cut(s) | 125, 401 |
| BciT130I | CCWGG | 3 cut(s) | 162, 293, 700 |
| BclI | TGATCA | 1 cut(s) | 604 |
| BcoDI | GTCTC | 2 cut(s) | 248, 680 |
| BcuI | ACTAGT | 1 cut(s) | 32 |
| BfaI | CTAG | 5 cut(s) | 33, 327, 587, 789, 797 |
| BfmI | CTRYAG | 1 cut(s) | 189 |
| BglII | AGATCT | 1 cut(s) | 721 |
| BisI | GCNGC | 2 cut(s) | 112, 189 |
| BlsI | GCNGC | 2 cut(s) | 113, 190 |
| BmcAI | AGTACT | 1 cut(s) | 31 |
| Bme1390I | CCNGG | 3 cut(s) | 162, 293, 700 |
| Bme18I | GGWCC | 3 cut(s) | 240, 289, 380 |
| BmeT110I | CYCGRG | 1 cut(s) | 669 |
| BmgT120I | GGNCC | 3 cut(s) | 240, 289, 380 |
| BmrFI | CCNGG | 3 cut(s) | 162, 293, 700 |
| BmsI | GCATC | 1 cut(s) | 87 |
| BpmI | CTGGAG | 3 cut(s) | 314, 822, 837 |
| Bpu10I | CCTNAGC | 1 cut(s) | 704 |
| BsaI | GGTCTC | 1 cut(s) | 680 |
| BsaJI | CCNNGG | 2 cut(s) | 117, 451 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 156, 186 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 705 |
| Bse1I | ACTGG | 2 cut(s) | 405, 820 |
| BseBI | CCWGG | 3 cut(s) | 162, 293, 700 |
| BseDI | CCNNGG | 2 cut(s) | 117, 451 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 705 |
| BseNI | ACTGG | 2 cut(s) | 405, 820 |
| BseRI | GAGGAG | 3 cut(s) | 560, 668, 819 |
| BseSI | GKGCMC | 1 cut(s) | 280 |
| BseXI | GCAGC | 2 cut(s) | 123, 175 |
| BseYI | CCCAGC | 1 cut(s) | 145 |
| BshFI | GGCC | 2 cut(s) | 450, 637 |
| BsiHKAI | GWGCWC | 1 cut(s) | 300 |
| BsiHKCI | CYCGRG | 1 cut(s) | 669 |
| BslFI | GGGAC | 2 cut(s) | 69, 409 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 705 |
| BsmAI | GTCTC | 2 cut(s) | 248, 680 |
| BsmFI | GGGAC | 2 cut(s) | 69, 409 |
| BsmI | GAATGC | 1 cut(s) | 210 |
| BsnI | GGCC | 2 cut(s) | 450, 637 |
| Bso31I | GGTCTC | 1 cut(s) | 680 |
| BsoBI | CYCGRG | 1 cut(s) | 669 |
| Bsp1286I | GDGCHC | 2 cut(s) | 280, 300 |
| Bsp143I | GATC | 5 cut(s) | 7, 486, 604, 721, 808 |
| BspACI | CCGC | 1 cut(s) | 302 |
| BspANI | GGCC | 2 cut(s) | 450, 637 |
| BspHI | TCATGA | 2 cut(s) | 100, 468 |
| BspMAI | CTGCAG | 1 cut(s) | 193 |
| BspQI | GCTCTTC | 1 cut(s) | 311 |
| BspTNI | GGTCTC | 1 cut(s) | 680 |
| BsrBI | CCGCTC | 1 cut(s) | 304 |
| BsrI | ACTGG | 2 cut(s) | 405, 820 |
| BssECI | CCNNGG | 2 cut(s) | 117, 451 |
| BssMI | GATC | 5 cut(s) | 7, 486, 604, 721, 808 |
| BssT1I | CCWWGG | 2 cut(s) | 117, 451 |
| Bst2UI | CCWGG | 3 cut(s) | 162, 293, 700 |
| Bst4CI | ACNGT | 1 cut(s) | 627 |
| Bst6I | CTCTTC | 3 cut(s) | 311, 422, 537 |
| BstDEI | CTNAG | 3 cut(s) | 211, 704, 828 |
| BstKTI | GATC | 5 cut(s) | 10, 489, 607, 724, 811 |
| BstMAI | GTCTC | 2 cut(s) | 248, 680 |
| BstMBI | GATC | 5 cut(s) | 7, 486, 604, 721, 808 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 447 |
| BstNI | CCWGG | 3 cut(s) | 162, 293, 700 |
| BstNSI | RCATGY | 2 cut(s) | 556, 574 |
| BstSCI | CCNGG | 3 cut(s) | 160, 291, 698 |
| BstSFI | CTRYAG | 1 cut(s) | 189 |
| BstSLI | GKGCMC | 1 cut(s) | 280 |
| BstV1I | GCAGC | 2 cut(s) | 123, 175 |
| BstX2I | RGATCY | 1 cut(s) | 721 |
| BstYI | RGATCY | 1 cut(s) | 721 |
| BsuRI | GGCC | 2 cut(s) | 450, 637 |
| BtsI | GCAGTG | 1 cut(s) | 198 |
| BtsIMutI | CAGTG | 2 cut(s) | 198, 398 |
| CciI | TCATGA | 2 cut(s) | 100, 468 |
| Cfr13I | GGNCC | 3 cut(s) | 240, 289, 380 |
| Csp6I | GTAC | 1 cut(s) | 30 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 638, 673 |
| CviAII | CATG | 4 cut(s) | 101, 469, 553, 571 |
| CviQI | GTAC | 1 cut(s) | 30 |
| DdeI | CTNAG | 3 cut(s) | 211, 704, 828 |
| DpnI | GATC | 5 cut(s) | 9, 488, 606, 723, 810 |
| DpnII | GATC | 5 cut(s) | 7, 486, 604, 721, 808 |
| EaeI | YGGCCR | 1 cut(s) | 448 |
| Eam1104I | CTCTTC | 3 cut(s) | 311, 422, 537 |
| EarI | CTCTTC | 3 cut(s) | 311, 422, 537 |
| Ecl136II | GAGCTC | 1 cut(s) | 298 |
| Eco130I | CCWWGG | 2 cut(s) | 117, 451 |
| Eco24I | GRGCYC | 1 cut(s) | 300 |
| Eco31I | GGTCTC | 1 cut(s) | 680 |
| Eco47I | GGWCC | 3 cut(s) | 240, 289, 380 |
| Eco53kI | GAGCTC | 1 cut(s) | 298 |
| Eco57I | CTGAAG | 1 cut(s) | 482 |
| Eco88I | CYCGRG | 1 cut(s) | 669 |
| EcoICRI | GAGCTC | 1 cut(s) | 298 |
| EcoO109I | RGGNCCY | 1 cut(s) | 289 |
| EcoRII | CCWGG | 3 cut(s) | 160, 291, 698 |
| EcoT14I | CCWWGG | 2 cut(s) | 117, 451 |
| EcoT38I | GRGCYC | 1 cut(s) | 300 |
| ErhI | CCWWGG | 2 cut(s) | 117, 451 |
| FaeI | CATG | 4 cut(s) | 104, 472, 556, 574 |
| FaqI | GGGAC | 2 cut(s) | 69, 409 |
| FatI | CATG | 4 cut(s) | 100, 468, 552, 570 |
| FauI | CCCGC | 1 cut(s) | 309 |
| FbaI | TGATCA | 1 cut(s) | 604 |
| FblI | GTMKAC | 1 cut(s) | 228 |
| Fnu4HI | GCNGC | 2 cut(s) | 112, 189 |
| FriOI | GRGCYC | 1 cut(s) | 300 |
| Fsp4HI | GCNGC | 2 cut(s) | 112, 189 |
| FspBI | CTAG | 5 cut(s) | 33, 327, 587, 789, 797 |
| GluI | GCNGC | 2 cut(s) | 112, 189 |
| GsaI | CCCAGC | 1 cut(s) | 149 |
| GsuI | CTGGAG | 3 cut(s) | 314, 822, 837 |
| HaeIII | GGCC | 2 cut(s) | 450, 637 |
| Hin1II | CATG | 4 cut(s) | 104, 472, 556, 574 |
| HincII | GTYRAC | 1 cut(s) | 73 |
| HindII | GTYRAC | 1 cut(s) | 73 |
| HindIII | AAGCTT | 2 cut(s) | 136, 359 |
| HinfI | GANTC | 3 cut(s) | 520, 695, 760 |
| HphI | GGTGA | 1 cut(s) | 17 |
| Hpy166II | GTNNAC | 4 cut(s) | 54, 73, 229, 388 |
| Hpy188I | TCNGA | 3 cut(s) | 349, 775, 813 |
| Hpy188III | TCNNGA | 4 cut(s) | 101, 201, 469, 841 |
| Hpy8I | GTNNAC | 4 cut(s) | 54, 73, 229, 388 |
| HpyAV | CCTTC | 1 cut(s) | 362 |
| HpyCH4III | ACNGT | 1 cut(s) | 627 |
| HpyCH4IV | ACGT | 1 cut(s) | 643 |
| HpyCH4V | TGCA | 3 cut(s) | 191, 332, 538 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 447 |
| HpyF3I | CTNAG | 3 cut(s) | 211, 704, 828 |
| HpySE526I | ACGT | 1 cut(s) | 643 |
| Hsp92II | CATG | 4 cut(s) | 104, 472, 556, 574 |
| Ksp22I | TGATCA | 1 cut(s) | 604 |
| Kzo9I | GATC | 5 cut(s) | 7, 486, 604, 721, 808 |
| LguI | GCTCTTC | 1 cut(s) | 311 |
| LmnI | GCTCC | 2 cut(s) | 295, 303 |
| Lsp1109I | GCAGC | 2 cut(s) | 123, 175 |
| LweI | GCATC | 1 cut(s) | 87 |
| MaeI | CTAG | 5 cut(s) | 33, 327, 587, 789, 797 |
| MaeII | ACGT | 1 cut(s) | 643 |
| MalI | GATC | 5 cut(s) | 9, 488, 606, 723, 810 |
| MbiI | CCGCTC | 1 cut(s) | 304 |
| MboI | GATC | 5 cut(s) | 7, 486, 604, 721, 808 |
| MboII | GAAGA | 6 cut(s) | 281, 298, 439, 554, 586, 629 |
| MflI | RGATCY | 1 cut(s) | 721 |
| MhlI | GDGCHC | 2 cut(s) | 280, 300 |
| MlsI | TGGCCA | 1 cut(s) | 450 |
| MluCI | AATT | 5 cut(s) | 245, 416, 477, 663, 768 |
| MluNI | TGGCCA | 1 cut(s) | 450 |
| MnlI | CCTC | 8 cut(s) | 97, 280, 423, 538, 606, 689, 797, 812 |
| Mox20I | TGGCCA | 1 cut(s) | 450 |
| MscI | TGGCCA | 1 cut(s) | 450 |
| MseI | TTAA | 1 cut(s) | 459 |
| MslI | CAYNNNNRTG | 1 cut(s) | 557 |
| Msp20I | TGGCCA | 1 cut(s) | 450 |
| MspR9I | CCNGG | 3 cut(s) | 162, 293, 700 |
| Mva1269I | GAATGC | 1 cut(s) | 210 |
| MvaI | CCWGG | 3 cut(s) | 162, 293, 700 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 447 |
| NdeII | GATC | 5 cut(s) | 7, 486, 604, 721, 808 |
| NlaIII | CATG | 4 cut(s) | 104, 472, 556, 574 |
| NspI | RCATGY | 2 cut(s) | 556, 574 |
| PagI | TCATGA | 2 cut(s) | 100, 468 |
| PciSI | GCTCTTC | 1 cut(s) | 311 |
| PctI | GAATGC | 1 cut(s) | 210 |
| PfeI | GAWTC | 3 cut(s) | 520, 695, 760 |
| PfoI | TCCNGGA | 1 cut(s) | 160 |
| PkrI | GCNGC | 2 cut(s) | 113, 190 |
| PpuMI | RGGWCCY | 1 cut(s) | 289 |
| PsiI | TTATAA | 1 cut(s) | 482 |
| Psp124BI | GAGCTC | 1 cut(s) | 300 |
| Psp1406I | AACGTT | 1 cut(s) | 643 |
| Psp5II | RGGWCCY | 1 cut(s) | 289 |
| Psp6I | CCWGG | 3 cut(s) | 160, 291, 698 |
| PspFI | CCCAGC | 1 cut(s) | 145 |
| PspGI | CCWGG | 3 cut(s) | 160, 291, 698 |
| PspPI | GGNCC | 3 cut(s) | 240, 289, 380 |
| PspPPI | RGGWCCY | 1 cut(s) | 289 |
| PstI | CTGCAG | 1 cut(s) | 193 |
| PsuI | RGATCY | 1 cut(s) | 721 |
| RsaI | GTAC | 1 cut(s) | 31 |
| RsaNI | GTAC | 1 cut(s) | 30 |
| RseI | CAYNNNNRTG | 1 cut(s) | 557 |
| SacI | GAGCTC | 1 cut(s) | 300 |
| SapI | GCTCTTC | 1 cut(s) | 311 |
| SaqAI | TTAA | 1 cut(s) | 459 |
| SatI | GCNGC | 2 cut(s) | 112, 189 |
| Sau3AI | GATC | 5 cut(s) | 7, 486, 604, 721, 808 |
| Sau96I | GGNCC | 3 cut(s) | 240, 289, 380 |
| ScaI | AGTACT | 1 cut(s) | 31 |
| ScrFI | CCNGG | 3 cut(s) | 162, 293, 700 |
| SduI | GDGCHC | 2 cut(s) | 280, 300 |
| SfaNI | GCATC | 1 cut(s) | 87 |
| SfcI | CTRYAG | 1 cut(s) | 189 |
| SinI | GGWCC | 3 cut(s) | 240, 289, 380 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 557 |
| SpeI | ACTAGT | 1 cut(s) | 32 |
| Sse9I | AATT | 5 cut(s) | 245, 416, 477, 663, 768 |
| SsiI | CCGC | 1 cut(s) | 302 |
| SspMI | CTAG | 5 cut(s) | 33, 327, 587, 789, 797 |
| SstI | GAGCTC | 1 cut(s) | 300 |
| StyD4I | CCNGG | 3 cut(s) | 160, 291, 698 |
| StyI | CCWWGG | 2 cut(s) | 117, 451 |
| TaaI | ACNGT | 1 cut(s) | 627 |
| TaiI | ACGT | 1 cut(s) | 646 |
| TasI | AATT | 5 cut(s) | 245, 416, 477, 663, 768 |
| TatI | WGTACW | 1 cut(s) | 29 |
| TfiI | GAWTC | 3 cut(s) | 520, 695, 760 |
| Tru1I | TTAA | 1 cut(s) | 459 |
| Tru9I | TTAA | 1 cut(s) | 459 |
| TscAI | CASTG | 2 cut(s) | 198, 405 |
| TseI | GCWGC | 2 cut(s) | 111, 188 |
| TspRI | CASTG | 2 cut(s) | 198, 405 |
| VpaK11BI | GGWCC | 3 cut(s) | 240, 289, 380 |
| XapI | RAATTY | 2 cut(s) | 416, 768 |
| XceI | RCATGY | 2 cut(s) | 556, 574 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 656 |
| XmiI | GTMKAC | 1 cut(s) | 228 |
| XspI | CTAG | 5 cut(s) | 33, 327, 587, 789, 797 |
| ZrmI | AGTACT | 1 cut(s) | 31 |