MD09G1250500.v1.1

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000351
Physical Location & Seq
Forward (+)
32080566 .. 32083766
3201 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1250500.v1.1.491

Sequence Viewer

Length: 1323 bp
ATGACAGACTCAGCCGGTGAGAGCAACACAAAAGCTGATGACTCCGTAATTCAGAGAAGTGAGAGAGAAATAAGTCATGTGGGTAACCAAAGTTGGAGCGGAAACGAAGAAATTGAAGACGAATTAGCGTCCTCGATTGAAAAAAAGCTTCCCAAGAAATCAACAGAGCCAGTTCATACTTGCGTATTTAGAGTCCCTACTAAACATTTCAGGGAGAATGAAAATGCTTTTTCCCCACAAGTGGTTTCAATCGGGCCGTATCACCACCAAAGAATAAAGTTTCAAGGCATGGAACAAACTAAGCTACGGTATTTGCGTTCCCTCCTCGACCGCAAACCAACTCCAGATACCAGTCTGGGGAAGTTTGTCAAAGAAATTAGAAGCAGAGAAGAGTTTCTTCGCAACTGCTATGACGAAAAGTTTGATCTGAGCAGCGATAATTTTGTAGAAATGATGGTGGTCGATGGTTGCTATATTTTAGAACTTCTCCGCAGGCCAAGTTTCCGTGTGCCAGGGTGGTTCTCCACATTTAAAAACGACTTGTTGCTGCTTGAAAACCAGCTTCCGTGGCAAGTTCTCGACTGTTTATTCAACCTCACAAAAACATCACAAGAGGTTTCTCTATATCAACTTACTAAGGGCCTTTCCGGAATGAAGCATAATCCCTTAGAGAAATGTGTCATGAAAAATTTACTTGACGCAGTAAGAAATTCGCTTGTTGGATCATTGCTGAACACAAGGTCTTATGATGTAGATTGGACTCCTATTCCCTCAGTGACACTTCTCGAGGAAATTGGAGTCAAATTTCAAAGTGCATCCAATGAGAGAGATCTGTCGGACATAACCTTCAACTTGGAAAATGGAGTGATGGAAATTCCATATGTGATGATTCGATCCAACACAGAATCCCTGTTCAGAAACCTCATAGCCTACGAACAGTGTCTGGACAACTTTGACAAATGTCCCATTTTGTCTTACGCCATGCTATTGAATCAGCTTATCAAGTCTGCCAAAGATTTAGACTCTCTCATTCAGAAAGGAATTATAGACACCGAGTTGAGCGAGGAGGACACTGTTTGTTTCTTCAATAGGCTTCGCTATAGCACTGAACGCATAAATTTCTTTTACTCTTCCCTCGCCCGGCAAGTGAATGAATGTTACCATCGTCGCTGGCTAAGGCGTTGGCTTGCAAGGATCAAAAGGGATTATCTATACAATCCGAAGTCAATCTTGTCACTTTTCTCAAATGCGGTCATCCTTGCTCTCATTCTTACCGTCCTGCAGACTGTATATGGTATTCTCGCCTACTACAAACCGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

441

Amino Acids

51.11

Weight (kDa)

6.44

Isoelectric Point (pI)

49.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 62 - 422 2.2e-93 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000351)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16420 FvH4_6g39730
malus_domestica MD02G1081100.v1.1 MD02G1081200.v1.1 MD09G1249600.v1.1 MD09G1250500.v1.1 MD09G1250900.v1.1 MD15G1081900.v1.1 MD15G1208800.v1.1 MD17G1242700.v1.1 MD17G1242800.v1.1 MD17G1242900.v1.1 MD17G1243200.v1.1
prunus_persica Prupe.3G130000_v2.0.a1 Prupe.3G130100_v2.0.a1 Prupe.3G130300_v2.0.a1 Prupe.3G130400_v2.0.a1 Prupe.3G130400_v2.0.a1 Prupe.3G130400_v2.0.a1 Prupe.3G130500_v2.0.a1 Prupe.3G136500_v2.0.a1 Prupe.3G141700_v2.0.a1 Prupe.3G141800_v2.0.a1 Prupe.3G141900_v2.0.a1 Prupe.3G155700_v2.0.a1 Prupe.7G207700_v2.0.a1 Prupe.7G207800_v2.0.a1 Prupe.7G207900_v2.0.a1 Prupe.7G207900_v2.0.a1 Prupe.7G208100_v2.0.a1 Prupe.7G208100_v2.0.a1 Prupe.7G208100_v2.0.a1 Prupe.7G208100_v2.0.a1
pyrus_communis pycom09g16640 pycom09g16690 pycom15g07650 pycom17g24550 pycom17g24560 pycom17g24570 pycom17g24670
rosa_chinensis RchiOBHm_Chr2g0153821 RchiOBHm_Chr2g0163501 RchiOBHm_Chr2g0163511 RchiOBHm_Chr2g0163521 RchiOBHm_Chr5g0063201 RchiOBHm_Chr7g0229231 RchiOBHm_Chr7g0229251
rosa_laevigata RLG00000001520 RLG00000020727 RLG00000020731 RLG00000021394 RLG00000022074 RLG00000023249
rosa_multiflora Rmu_sc0000235.1_g000044 Rmu_sc0000693.1_g000020 Rmu_sc0000693.1_g000032 Rmu_sc0002053.1_g000010 Rmu_sc0002053.1_g000011 Rmu_sc0003887.1_g000020 Rmu_sc0004278.1_g000001 Rmu_sc0012759.1_g000001
rosa_roxburghii Rroxscaffold_2G00087170 Rroxscaffold_2G00087180 Rroxscaffold_2G00087190 Rroxscaffold_2G00087230 Rroxscaffold_2G00087250 Rroxscaffold_2G00087320 Rroxscaffold_2G00094720 Rroxscaffold_3G00230450 Rroxscaffold_3G00230460
rosa_rugosa Rorug02G0442300.1 Rorug02G0442400 Rorug02G0503400 Rorug02G0503500 Rorug02G0560600 Rorug05G0355000 Rorug05G0355100 Rorug07G0258000
rosa_samantha Rh2AG185500 Rh2AG506100 Rh2AG506200 Rh2AG570300 Rh2AG570400 Rh2BG515600 Rh2BG582500 Rh2BG582600 Rh2BG582700 Rh2CG491600 Rh2CG491700 Rh2CG552300 Rh2CG552400 Rh2CG617700 Rh2CG617800 Rh2DG528100 Rh2DG592200 Rh2DG592300 Rh2DG592400 Rh2DG665500 Rh5CG234300 Rh5DG460600 Rh7AG407800 Rh7BG387600 Rh7CG426800 Rh7CG427000 Rh7DG402900
rosa_wichuraiana Rw0G001890 Rw2G041540 Rw2G047220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 99
AccIII TCCGGA 1 cut(s) 647
AciI CCGC 4 cut(s) 99, 331, 490, 1250
AclWI GGATC 3 cut(s) 730, 888, 1202
AcsI RAATTY 5 cut(s) 688, 709, 803, 873, 1117
AfiI CCNNNNNNNGG 3 cut(s) 241, 357, 1140
AjnI CCWGG 1 cut(s) 511
AluBI AGCT 5 cut(s) 35, 148, 304, 562, 997
AluI AGCT 5 cut(s) 35, 148, 304, 562, 997
AlwI GGATC 3 cut(s) 730, 888, 1202
AlwNI CAGNNNCTG 1 cut(s) 943
Ama87I CYCGRG 1 cut(s) 785
Aor13HI TCCGGA 1 cut(s) 647
AoxI GGCC 3 cut(s) 254, 494, 640
ApeKI GCWGC 2 cut(s) 432, 547
ApoI RAATTY 5 cut(s) 688, 709, 803, 873, 1117
ArsI GACNNNNNNTTYG 2 cut(s) 404, 436
Asp700I GAANNNNTTC 1 cut(s) 393
AspS9I GGNCC 2 cut(s) 254, 640
AsuC2I CCSGG 1 cut(s) 1141
AsuHPI GGTGA 2 cut(s) 29, 254
AvaI CYCGRG 1 cut(s) 785
BbsI GAAGAC 1 cut(s) 123
BbvI GCAGC 2 cut(s) 444, 534
BccI CCATC 4 cut(s) 448, 458, 862, 1170
BceAI ACGGC 1 cut(s) 241
BciT130I CCWGG 1 cut(s) 513
BcnI CCSGG 1 cut(s) 1141
BfmI CTRYAG 2 cut(s) 1099, 1280
BglII AGATCT 1 cut(s) 829
BisI GCNGC 2 cut(s) 433, 548
BlsI GCNGC 2 cut(s) 434, 549
Bme1390I CCNGG 2 cut(s) 513, 1141
BmeT110I CYCGRG 1 cut(s) 785
BmgT120I GGNCC 2 cut(s) 254, 640
BmrFI CCNGG 2 cut(s) 513, 1141
BmsI GCATC 1 cut(s) 824
BoxI GACNNNNGTC 1 cut(s) 960
BpiI GAAGAC 1 cut(s) 123
BpmI CTGGAG 1 cut(s) 327
Bpu10I CCTNAGC 1 cut(s) 1175
BpuMI CCSGG 1 cut(s) 1141
BsaJI CCNNGG 2 cut(s) 512, 566
BsaWI WCCGGW 1 cut(s) 647
Bsc4I CCNNNNNNNGG 3 cut(s) 241, 357, 1140
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 2 cut(s) 170, 351
Bse3DI GCAATG 1 cut(s) 725
BseAI TCCGGA 1 cut(s) 647
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 2 cut(s) 512, 566
BseGI GGATG 2 cut(s) 815, 1254
BseLI CCNNNNNNNGG 3 cut(s) 241, 357, 1140
BseMI GCAATG 1 cut(s) 725
BseMII CTCAG 3 cut(s) 24, 419, 786
BseNI ACTGG 2 cut(s) 170, 351
BseRI GAGGAG 2 cut(s) 314, 1079
BseXI GCAGC 2 cut(s) 444, 534
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 3 cut(s) 256, 496, 642
BsiEI CGRYCG 1 cut(s) 331
BsiHKCI CYCGRG 1 cut(s) 785
BsiSI CCGG 3 cut(s) 15, 648, 1141
BslFI GGGAC 2 cut(s) 179, 948
BslI CCNNNNNNNGG 3 cut(s) 241, 357, 1140
BsmFI GGGAC 2 cut(s) 179, 948
BsnI GGCC 3 cut(s) 256, 496, 642
BsoBI CYCGRG 1 cut(s) 785
Bsp13I TCCGGA 1 cut(s) 647
Bsp143I GATC 5 cut(s) 424, 722, 829, 893, 1194
BspACI CCGC 4 cut(s) 99, 331, 490, 1250
BspANI GGCC 3 cut(s) 256, 496, 642
BspCNI CTCAG 3 cut(s) 23, 420, 785
BspEI TCCGGA 1 cut(s) 647
BspHI TCATGA 1 cut(s) 681
BspMAI CTGCAG 1 cut(s) 1284
BspPI GGATC 3 cut(s) 730, 888, 1202
BsrBI CCGCTC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 725
BsrFI RCCGGY 1 cut(s) 14
BsrI ACTGG 2 cut(s) 170, 351
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 2 cut(s) 512, 566
BssMI GATC 5 cut(s) 424, 722, 829, 893, 1194
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 6 cut(s) 309, 584, 939, 1075, 1276, 1288
Bst6I CTCTTC 2 cut(s) 384, 1135
BstC8I GCNNGC 3 cut(s) 494, 1172, 1188
BstDEI CTNAG 7 cut(s) 10, 300, 428, 636, 667, 772, 1175
BstDSI CCRYGG 1 cut(s) 566
BstEII GGTNACC 1 cut(s) 83
BstF5I GGATG 2 cut(s) 815, 1254
BstKTI GATC 5 cut(s) 427, 725, 832, 896, 1197
BstMBI GATC 5 cut(s) 424, 722, 829, 893, 1194
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 2 cut(s) 568, 1110
BstNI CCWGG 1 cut(s) 513
BstPAI GACNNNNGTC 1 cut(s) 960
BstPI GGTNACC 1 cut(s) 83
BstSCI CCNGG 2 cut(s) 511, 1139
BstSFI CTRYAG 2 cut(s) 1099, 1280
BstV1I GCAGC 2 cut(s) 444, 534
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 829
BstYI RGATCY 1 cut(s) 829
BsuRI GGCC 3 cut(s) 256, 496, 642
BtgI CCRYGG 1 cut(s) 566
BtsCI GGATG 2 cut(s) 815, 1254
BtsIMutI CAGTG 4 cut(s) 780, 944, 1071, 1104
Cac8I GCNNGC 3 cut(s) 494, 1172, 1188
CaiI CAGNNNCTG 1 cut(s) 943
CciI TCATGA 1 cut(s) 681
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 2 cut(s) 254, 640
CseI GACGC 2 cut(s) 117, 707
CviAII CATG 4 cut(s) 77, 289, 682, 982
DdeI CTNAG 7 cut(s) 10, 300, 428, 636, 667, 772, 1175
DpnI GATC 5 cut(s) 426, 724, 831, 895, 1196
DpnII GATC 5 cut(s) 424, 722, 829, 893, 1194
DraI TTTAAA 1 cut(s) 532
Eam1104I CTCTTC 2 cut(s) 384, 1135
EarI CTCTTC 2 cut(s) 384, 1135
Eco88I CYCGRG 1 cut(s) 785
Eco91I GGTNACC 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 640
EcoO65I GGTNACC 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 511
FaeI CATG 4 cut(s) 80, 292, 685, 985
FalI AAGNNNNNCTT 4 cut(s) 381, 413, 1214, 1246
FaqI GGGAC 2 cut(s) 179, 948
FatI CATG 4 cut(s) 76, 288, 681, 981
FauNDI CATATG 1 cut(s) 880
Fnu4HI GCNGC 2 cut(s) 433, 548
FokI GGATG 2 cut(s) 802, 1241
Fsp4HI GCNGC 2 cut(s) 433, 548
GluI GCNGC 2 cut(s) 433, 548
GsuI CTGGAG 1 cut(s) 327
HaeIII GGCC 3 cut(s) 256, 496, 642
HapII CCGG 3 cut(s) 15, 648, 1141
HgaI GACGC 2 cut(s) 117, 707
Hin1II CATG 4 cut(s) 80, 292, 685, 985
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 9 cut(s) 8, 41, 192, 760, 798, 889, 905, 991, 1022
HpaII CCGG 3 cut(s) 15, 648, 1141
HphI GGTGA 2 cut(s) 29, 254
Hpy188I TCNGA 6 cut(s) 54, 429, 838, 917, 1035, 1221
Hpy188III TCNNGA 6 cut(s) 344, 578, 648, 682, 785, 944
Hpy99I CGWCG 1 cut(s) 1170
HpyAV CCTTC 1 cut(s) 856
HpyCH4III ACNGT 6 cut(s) 309, 584, 939, 1075, 1276, 1288
HpyCH4V TGCA 3 cut(s) 815, 1190, 1282
HpyF10VI GCNNNNNNNGC 2 cut(s) 568, 1110
HpyF3I CTNAG 7 cut(s) 10, 300, 428, 636, 667, 772, 1175
Hsp92II CATG 4 cut(s) 80, 292, 685, 985
Kpn2I TCCGGA 1 cut(s) 647
Kzo9I GATC 5 cut(s) 424, 722, 829, 893, 1194
LmnI GCTCC 1 cut(s) 96
Lsp1109I GCAGC 2 cut(s) 444, 534
LweI GCATC 1 cut(s) 824
MaeIII GTNAC 4 cut(s) 83, 775, 1157, 1233
MalI GATC 5 cut(s) 426, 724, 831, 895, 1196
MbiI CCGCTC 1 cut(s) 99
MboI GATC 5 cut(s) 424, 722, 829, 893, 1194
MboII GAAGA 6 cut(s) 119, 128, 389, 401, 1075, 1122
MflI RGATCY 1 cut(s) 829
MlyI GAGTC 6 cut(s) 2, 35, 201, 754, 807, 1016
MmeI TCCRAC 4 cut(s) 74, 700, 816, 921
MroI TCCGGA 1 cut(s) 647
MroXI GAANNNNTTC 1 cut(s) 393
MseI TTAA 1 cut(s) 531
MspI CCGG 3 cut(s) 15, 648, 1141
MspR9I CCNGG 2 cut(s) 513, 1141
MvaI CCWGG 1 cut(s) 513
MwoI GCNNNNNNNGC 2 cut(s) 568, 1110
NciI CCSGG 1 cut(s) 1141
NdeI CATATG 1 cut(s) 880
NdeII GATC 5 cut(s) 424, 722, 829, 893, 1194
NlaIII CATG 4 cut(s) 80, 292, 685, 985
NmuCI GTSAC 2 cut(s) 775, 1233
PaeR7I CTCGAG 1 cut(s) 785
PagI TCATGA 1 cut(s) 681
PcsI WCGNNNNNNNCGW 1 cut(s) 313
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 3 cut(s) 889, 905, 991
PkrI GCNGC 2 cut(s) 434, 549
PleI GAGTC 6 cut(s) 2, 35, 200, 754, 806, 1016
PpsI GAGTC 6 cut(s) 2, 35, 200, 754, 806, 1016
PshAI GACNNNNGTC 1 cut(s) 960
Psp6I CCWGG 1 cut(s) 511
PspEI GGTNACC 1 cut(s) 83
PspGI CCWGG 1 cut(s) 511
PspPI GGNCC 2 cut(s) 254, 640
PstI CTGCAG 1 cut(s) 1284
PstNI CAGNNNCTG 1 cut(s) 943
PsuI RGATCY 1 cut(s) 829
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 2 cut(s) 433, 548
Sau3AI GATC 5 cut(s) 424, 722, 829, 893, 1194
Sau96I GGNCC 2 cut(s) 254, 640
SchI GAGTC 6 cut(s) 2, 35, 201, 754, 807, 1016
ScrFI CCNGG 2 cut(s) 513, 1141
SfaNI GCATC 1 cut(s) 824
SfcI CTRYAG 2 cut(s) 1099, 1280
Sfr274I CTCGAG 1 cut(s) 785
SlaI CTCGAG 1 cut(s) 785
SmlI CTYRAG 1 cut(s) 785
SmoI CTYRAG 1 cut(s) 785
SsiI CCGC 4 cut(s) 99, 331, 490, 1250
StyD4I CCNGG 2 cut(s) 511, 1139
TaaI ACNGT 6 cut(s) 309, 584, 939, 1075, 1276, 1288
TaqI TCGA 6 cut(s) 134, 327, 462, 579, 786, 892
TfiI GAWTC 3 cut(s) 889, 905, 991
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 4 cut(s) 780, 944, 1078, 1111
TseFI GTSAC 2 cut(s) 775, 1233
TseI GCWGC 2 cut(s) 432, 547
Tsp45I GTSAC 2 cut(s) 775, 1233
TspDTI ATGAA 5 cut(s) 164, 234, 668, 698, 1167
TspGWI ACGGA 3 cut(s) 34, 494, 555
TspRI CASTG 4 cut(s) 780, 944, 1078, 1111
XapI RAATTY 5 cut(s) 688, 709, 803, 873, 1117
XhoI CTCGAG 1 cut(s) 785
XmnI GAANNNNTTC 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.