Rw2G041540

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000351
Physical Location & Seq
Reverse (-)
67575657 .. 67577026
1370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G041540.1

Sequence Viewer

Length: 1329 bp
ATGACAGAGAGCAATGTGAATGAGCACTACTTGATCGAAATTAAAGAAGAGGAAGAGAGTGTGATAGCAGCAAACAATGTGAAAAACCAAACTTCGAGTGGAAATCAAAATGAGGAGAGTGTCAACATAGCATCCTCCATTAGAAGAAAGCTACCTAGCAAAGCACCATCACCACCTAGTACTTGCATATACAGAGTCCCTGATGCACTAAAGAAGCTTCACGAAAATGATTTTGTTCCTAGTTTAGTTTCAATTGGACCATTTCACCATGGCACAAAGAATCTGCAAGCCATGGAAGAATTTAAGCTTTGGTACTTGCACCAGCTGCTTGATCGAAAACCCAATCTTGAGACCAAATTGGAGCACATTGTTGAAAAAATCAGAAGCATGGAAGACTATTGTCGTGAATGCTATAACGAAAAACTTGATCATCTGAGCAGTGAAGCATTTGTAGAAATGATGGTGGTTGACGGTTGCTTTATTCTTGAATACTACCGCAAAAGAAAAGGAGAAGTGCCTGCAGACGCGGATGATCCACTGTTCAATATTAGTTTAGCAATGTCGCCAACACTTACAAGTGACTTGGTTATGCTAGAAAACCAGTTGCCTTGGAGAGTTCTTGACTGTTTATTCCAACTCACAAACGTACCTAATGAATGTTCAAGTCACTATCTGAGCTTACTCGCAGTTCAGACATTGGAAAGCAGACATTTACTTGACAATGTCAGGATCCTTTCTGCTGAATTAGACAAGAAAGAGGAGATTATGCCATTTTGGGACTCTATGCCCTCTGCATCGCAACTTCAGCAAATTGGAGTCATATTTCAACCCAACAGAAATAACATCAACATAAACATAACCTTCCTCGAGGGAGTGATGGAGATTGCACCAATAACCATCCAGGGTGATAATCCTGTCTTTAGAAACCTCATCGCCTTGGAACAGTGTGAATCAGGGCCCCATAAGTATCAAATTAGCACTTATGCCAGGGCATTGGGTGACCTTATTAAATCTAGTAAAGATGTGGAGTTTCTTATGGAGAAAGAGATTATTCATACCAGTTTAAACAAGGAAGACATAGCCGATTTCTTCAACAACATTTGCAATGGTTTTGCAACTTCCTCCTGCTGCAAGTCTTTTTGGCAAGTTTCCTTGACAGTGAGACAGTATTATAAACAACATTGGCTACAAAGATTGATAGTATACATCAAACATAACTACTTGTACAATCCCTCATCAATATGGTCAGTTTCTAATGCAATTGTCCTTATTATAGTTCTTACGGTCCTGCAAACCATATATTCGGTCCTCTCTTACTACAAGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

442

Amino Acids

50.83

Weight (kDa)

5.45

Isoelectric Point (pI)

55.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 63 - 425 6.1e-90 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000351)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16420 FvH4_6g39730
malus_domestica MD02G1081100.v1.1 MD02G1081200.v1.1 MD09G1249600.v1.1 MD09G1250500.v1.1 MD09G1250900.v1.1 MD15G1081900.v1.1 MD15G1208800.v1.1 MD17G1242700.v1.1 MD17G1242800.v1.1 MD17G1242900.v1.1 MD17G1243200.v1.1
prunus_persica Prupe.3G130000_v2.0.a1 Prupe.3G130100_v2.0.a1 Prupe.3G130300_v2.0.a1 Prupe.3G130400_v2.0.a1 Prupe.3G130400_v2.0.a1 Prupe.3G130400_v2.0.a1 Prupe.3G130500_v2.0.a1 Prupe.3G136500_v2.0.a1 Prupe.3G141700_v2.0.a1 Prupe.3G141800_v2.0.a1 Prupe.3G141900_v2.0.a1 Prupe.3G155700_v2.0.a1 Prupe.7G207700_v2.0.a1 Prupe.7G207800_v2.0.a1 Prupe.7G207900_v2.0.a1 Prupe.7G207900_v2.0.a1 Prupe.7G208100_v2.0.a1 Prupe.7G208100_v2.0.a1 Prupe.7G208100_v2.0.a1 Prupe.7G208100_v2.0.a1
pyrus_communis pycom09g16640 pycom09g16690 pycom15g07650 pycom17g24550 pycom17g24560 pycom17g24570 pycom17g24670
rosa_chinensis RchiOBHm_Chr2g0153821 RchiOBHm_Chr2g0163501 RchiOBHm_Chr2g0163511 RchiOBHm_Chr2g0163521 RchiOBHm_Chr5g0063201 RchiOBHm_Chr7g0229231 RchiOBHm_Chr7g0229251
rosa_laevigata RLG00000001520 RLG00000020727 RLG00000020731 RLG00000021394 RLG00000022074 RLG00000023249
rosa_multiflora Rmu_sc0000235.1_g000044 Rmu_sc0000693.1_g000020 Rmu_sc0000693.1_g000032 Rmu_sc0002053.1_g000010 Rmu_sc0002053.1_g000011 Rmu_sc0003887.1_g000020 Rmu_sc0004278.1_g000001 Rmu_sc0012759.1_g000001
rosa_roxburghii Rroxscaffold_2G00087170 Rroxscaffold_2G00087180 Rroxscaffold_2G00087190 Rroxscaffold_2G00087230 Rroxscaffold_2G00087250 Rroxscaffold_2G00087320 Rroxscaffold_2G00094720 Rroxscaffold_3G00230450 Rroxscaffold_3G00230460
rosa_rugosa Rorug02G0442300.1 Rorug02G0442400 Rorug02G0503400 Rorug02G0503500 Rorug02G0560600 Rorug05G0355000 Rorug05G0355100 Rorug07G0258000
rosa_samantha Rh2AG185500 Rh2AG506100 Rh2AG506200 Rh2AG570300 Rh2AG570400 Rh2BG515600 Rh2BG582500 Rh2BG582600 Rh2BG582700 Rh2CG491600 Rh2CG491700 Rh2CG552300 Rh2CG552400 Rh2CG617700 Rh2CG617800 Rh2DG528100 Rh2DG592200 Rh2DG592300 Rh2DG592400 Rh2DG665500 Rh5CG234300 Rh5DG460600 Rh7AG407800 Rh7BG387600 Rh7CG426800 Rh7CG427000 Rh7DG402900
rosa_wichuraiana Rw0G001890 Rw2G041540 Rw2G047220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1173
AbsI CCTCGAGG 1 cut(s) 866
AccI GTMKAC 1 cut(s) 1203
AccII CGCG 1 cut(s) 527
AciI CCGC 2 cut(s) 496, 527
AclWI GGATC 3 cut(s) 527, 724, 737
AcsI RAATTY 1 cut(s) 299
AcuI CTGAAG 1 cut(s) 788
AfaI GTAC 4 cut(s) 181, 314, 648, 1226
AgsI TTSAA 7 cut(s) 252, 374, 488, 544, 663, 827, 1093
AjnI CCWGG 2 cut(s) 900, 986
AluBI AGCT 5 cut(s) 151, 217, 307, 325, 678
AluI AGCT 5 cut(s) 151, 217, 307, 325, 678
Alw21I GWGCWC 2 cut(s) 27, 366
Alw26I GTCTC 2 cut(s) 344, 1156
AlwI GGATC 3 cut(s) 527, 724, 737
Ama87I CYCGRG 1 cut(s) 866
AoxI GGCC 1 cut(s) 956
ApaI GGGCCC 1 cut(s) 960
ApeKI GCWGC 3 cut(s) 68, 325, 1128
ApoI RAATTY 1 cut(s) 299
AspS9I GGNCC 5 cut(s) 257, 956, 957, 1285, 1306
AsuHPI GGTGA 4 cut(s) 162, 257, 917, 1010
AvaI CYCGRG 1 cut(s) 866
AvaII GGWCC 3 cut(s) 257, 1285, 1306
BaeGI GKGCMC 1 cut(s) 960
BaeI ACNNNNGTAYC 2 cut(s) 630, 663
BamHI GGATCC 1 cut(s) 729
BanII GRGCYC 1 cut(s) 960
BbsI GAAGAC 2 cut(s) 399, 1080
Bbv12I GWGCWC 2 cut(s) 27, 366
BbvI GCAGC 3 cut(s) 80, 312, 1115
BccI CCATC 4 cut(s) 175, 454, 871, 905
BciT130I CCWGG 2 cut(s) 902, 988
BclI TGATCA 1 cut(s) 427
BcoDI GTCTC 2 cut(s) 344, 1156
BfaI CTAG 5 cut(s) 156, 177, 240, 593, 1014
BfmI CTRYAG 1 cut(s) 519
BisI GCNGC 3 cut(s) 69, 326, 1129
BlsI GCNGC 3 cut(s) 70, 327, 1130
BmcAI AGTACT 1 cut(s) 181
Bme1390I CCNGG 2 cut(s) 902, 988
Bme18I GGWCC 3 cut(s) 257, 1285, 1306
BmeT110I CYCGRG 1 cut(s) 866
BmgT120I GGNCC 5 cut(s) 257, 956, 957, 1285, 1306
BmiI GGNNCC 3 cut(s) 731, 958, 959
BmrFI CCNGG 2 cut(s) 902, 988
BmsI GCATC 3 cut(s) 140, 193, 803
BoxI GACNNNNGTC 1 cut(s) 399
BpiI GAAGAC 2 cut(s) 399, 1080
BpuEI CTTGAG 1 cut(s) 368
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 6 cut(s) 268, 291, 608, 901, 936, 987
Bse1I ACTGG 2 cut(s) 601, 1059
Bse3DI GCAATG 3 cut(s) 19, 564, 1111
BseBI CCWGG 2 cut(s) 902, 988
BseDI CCNNGG 6 cut(s) 268, 291, 608, 901, 936, 987
BseGI GGATG 3 cut(s) 131, 535, 897
BseMI GCAATG 3 cut(s) 19, 564, 1111
BseMII CTCAG 2 cut(s) 425, 665
BseNI ACTGG 2 cut(s) 601, 1059
BseRI GAGGAG 2 cut(s) 128, 773
BseSI GKGCMC 1 cut(s) 960
BseXI GCAGC 3 cut(s) 80, 312, 1115
Bsh1236I CGCG 1 cut(s) 527
BshFI GGCC 1 cut(s) 958
BsiHKAI GWGCWC 2 cut(s) 27, 366
BsiHKCI CYCGRG 1 cut(s) 866
BslFI GGGAC 2 cut(s) 182, 791
BsmAI GTCTC 2 cut(s) 344, 1156
BsmFI GGGAC 2 cut(s) 182, 791
BsmI GAATGC 1 cut(s) 413
BsnI GGCC 1 cut(s) 958
Bso31I GGTCTC 1 cut(s) 344
BsoBI CYCGRG 1 cut(s) 866
Bsp120I GGGCCC 1 cut(s) 956
Bsp1286I GDGCHC 3 cut(s) 27, 366, 960
Bsp1407I TGTACA 1 cut(s) 1224
Bsp143I GATC 5 cut(s) 33, 331, 427, 532, 729
Bsp19I CCATGG 2 cut(s) 268, 291
BspACI CCGC 2 cut(s) 496, 527
BspANI GGCC 1 cut(s) 958
BspCNI CTCAG 2 cut(s) 426, 666
BspFNI CGCG 1 cut(s) 527
BspLI GGNNCC 3 cut(s) 731, 958, 959
BspMAI CTGCAG 1 cut(s) 523
BspPI GGATC 3 cut(s) 527, 724, 737
BspTNI GGTCTC 1 cut(s) 344
BsrDI GCAATG 3 cut(s) 19, 564, 1111
BsrGI TGTACA 1 cut(s) 1224
BsrI ACTGG 2 cut(s) 601, 1059
BssECI CCNNGG 6 cut(s) 268, 291, 608, 901, 936, 987
BssMI GATC 5 cut(s) 33, 331, 427, 532, 729
BssNAI GTATAC 1 cut(s) 1204
BssT1I CCWWGG 4 cut(s) 268, 291, 608, 936
Bst1107I GTATAC 1 cut(s) 1204
Bst2UI CCWGG 2 cut(s) 902, 988
Bst4CI ACNGT 7 cut(s) 473, 540, 626, 945, 1159, 1167, 1285
Bst6I CTCTTC 2 cut(s) 42, 48
BstAPI GCANNNNNTGC 1 cut(s) 325
BstAUI TGTACA 1 cut(s) 1224
BstC8I GCNNGC 2 cut(s) 288, 519
BstDEI CTNAG 2 cut(s) 434, 674
BstDSI CCRYGG 2 cut(s) 268, 291
BstEII GGTNACC 1 cut(s) 998
BstF5I GGATG 3 cut(s) 131, 535, 897
BstFNI CGCG 1 cut(s) 527
BstKTI GATC 5 cut(s) 36, 334, 430, 535, 732
BstMAI GTCTC 2 cut(s) 344, 1156
BstMBI GATC 5 cut(s) 33, 331, 427, 532, 729
BstMWI GCNNNNNNNGC 2 cut(s) 325, 805
BstNI CCWGG 2 cut(s) 902, 988
BstPAI GACNNNNGTC 1 cut(s) 399
BstPI GGTNACC 1 cut(s) 998
BstSCI CCNGG 2 cut(s) 900, 986
BstSFI CTRYAG 1 cut(s) 519
BstSLI GKGCMC 1 cut(s) 960
BstUI CGCG 1 cut(s) 527
BstV1I GCAGC 3 cut(s) 80, 312, 1115
BstV2I GAAGAC 2 cut(s) 399, 1080
BstX2I RGATCY 1 cut(s) 729
BstXI CCANNNNNNTGG 1 cut(s) 994
BstYI RGATCY 1 cut(s) 729
BstZ17I GTATAC 1 cut(s) 1204
BsuRI GGCC 1 cut(s) 958
BtgI CCRYGG 2 cut(s) 268, 291
BtgZI GCGATG 2 cut(s) 780, 916
BtsCI GGATG 3 cut(s) 131, 535, 897
BtsI GCAGTG 1 cut(s) 445
BtsIMutI CAGTG 4 cut(s) 445, 536, 950, 1164
Cac8I GCNNGC 2 cut(s) 288, 519
Cfr13I GGNCC 5 cut(s) 257, 956, 957, 1285, 1306
CseI GACGC 1 cut(s) 533
Csp6I GTAC 4 cut(s) 180, 313, 647, 1225
CviAII CATG 3 cut(s) 269, 292, 388
CviJI RGCY 9 cut(s) 151, 217, 290, 307, 325, 678, 958, 1082, 1186
CviKI_1 RGCY 9 cut(s) 151, 217, 290, 307, 325, 678, 958, 1082, 1186
CviQI GTAC 4 cut(s) 180, 313, 647, 1225
DdeI CTNAG 2 cut(s) 434, 674
DpnI GATC 5 cut(s) 35, 333, 429, 534, 731
DpnII GATC 5 cut(s) 33, 331, 427, 532, 729
DraI TTTAAA 1 cut(s) 1065
Eam1104I CTCTTC 2 cut(s) 42, 48
EarI CTCTTC 2 cut(s) 42, 48
Eco130I CCWWGG 4 cut(s) 268, 291, 608, 936
Eco24I GRGCYC 1 cut(s) 960
Eco31I GGTCTC 1 cut(s) 344
Eco47I GGWCC 3 cut(s) 257, 1285, 1306
Eco57I CTGAAG 1 cut(s) 788
Eco88I CYCGRG 1 cut(s) 866
Eco91I GGTNACC 1 cut(s) 998
EcoO109I RGGNCCY 2 cut(s) 956, 957
EcoO65I GGTNACC 1 cut(s) 998
EcoRII CCWGG 2 cut(s) 900, 986
EcoT14I CCWWGG 4 cut(s) 268, 291, 608, 936
EcoT38I GRGCYC 1 cut(s) 960
ErhI CCWWGG 4 cut(s) 268, 291, 608, 936
FaeI CATG 3 cut(s) 272, 295, 391
FaqI GGGAC 2 cut(s) 182, 791
FatI CATG 3 cut(s) 268, 291, 387
FbaI TGATCA 1 cut(s) 427
FblI GTMKAC 1 cut(s) 1203
Fnu4HI GCNGC 3 cut(s) 69, 326, 1129
FokI GGATG 3 cut(s) 118, 542, 884
FriOI GRGCYC 1 cut(s) 960
Fsp4HI GCNGC 3 cut(s) 69, 326, 1129
FspBI CTAG 5 cut(s) 156, 177, 240, 593, 1014
GluI GCNGC 3 cut(s) 69, 326, 1129
HaeIII GGCC 1 cut(s) 958
HgaI GACGC 1 cut(s) 533
Hin1II CATG 3 cut(s) 272, 295, 391
HincII GTYRAC 2 cut(s) 124, 469
HindII GTYRAC 2 cut(s) 124, 469
HindIII AAGCTT 2 cut(s) 215, 305
HinfI GANTC 5 cut(s) 195, 280, 779, 816, 950
HphI GGTGA 4 cut(s) 162, 257, 917, 1010
Hpy166II GTNNAC 3 cut(s) 124, 469, 1204
Hpy188I TCNGA 4 cut(s) 383, 435, 675, 693
Hpy188III TCNNGA 6 cut(s) 221, 347, 404, 485, 620, 727
Hpy8I GTNNAC 3 cut(s) 124, 469, 1204
HpyAV CCTTC 1 cut(s) 871
HpyCH4III ACNGT 7 cut(s) 473, 540, 626, 945, 1159, 1167, 1285
HpyCH4IV ACGT 1 cut(s) 645
HpyF10VI GCNNNNNNNGC 2 cut(s) 325, 805
HpyF3I CTNAG 2 cut(s) 434, 674
HpySE526I ACGT 1 cut(s) 645
Hsp92II CATG 3 cut(s) 272, 295, 391
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 5 cut(s) 33, 331, 427, 532, 729
LmnI GCTCC 1 cut(s) 361
Lsp1109I GCAGC 3 cut(s) 80, 312, 1115
LweI GCATC 3 cut(s) 140, 193, 803
MaeI CTAG 5 cut(s) 156, 177, 240, 593, 1014
MaeII ACGT 1 cut(s) 645
MaeIII GTNAC 3 cut(s) 578, 665, 998
MalI GATC 5 cut(s) 35, 333, 429, 534, 731
MboI GATC 5 cut(s) 33, 331, 427, 532, 729
MboII GAAGA 7 cut(s) 59, 65, 156, 308, 404, 1081, 1085
MfeI CAATTG 2 cut(s) 252, 1260
MflI RGATCY 1 cut(s) 729
MhlI GDGCHC 3 cut(s) 27, 366, 960
MluCI AATT 8 cut(s) 39, 252, 299, 356, 743, 810, 972, 1260
MlyI GAGTC 3 cut(s) 204, 773, 825
MmeI TCCRAC 1 cut(s) 658
MseI TTAA 4 cut(s) 42, 303, 1008, 1064
MslI CAYNNNNRTG 2 cut(s) 225, 1240
MspA1I CMGCKG 1 cut(s) 325
MspR9I CCNGG 2 cut(s) 902, 988
MssI GTTTAAAC 1 cut(s) 1065
MunI CAATTG 2 cut(s) 252, 1260
Mva1269I GAATGC 1 cut(s) 413
MvaI CCWGG 2 cut(s) 902, 988
MvnI CGCG 1 cut(s) 527
MwoI GCNNNNNNNGC 2 cut(s) 325, 805
NcoI CCATGG 2 cut(s) 268, 291
NdeII GATC 5 cut(s) 33, 331, 427, 532, 729
NlaIII CATG 3 cut(s) 272, 295, 391
NlaIV GGNNCC 3 cut(s) 731, 958, 959
NmuCI GTSAC 3 cut(s) 578, 665, 998
PaeR7I CTCGAG 1 cut(s) 866
PctI GAATGC 1 cut(s) 413
PfeI GAWTC 2 cut(s) 280, 950
PflFI GACNNNGTC 1 cut(s) 722
PkrI GCNGC 3 cut(s) 70, 327, 1130
PleI GAGTC 3 cut(s) 203, 773, 824
PmeI GTTTAAAC 1 cut(s) 1065
PpsI GAGTC 3 cut(s) 203, 773, 824
PshAI GACNNNNGTC 1 cut(s) 399
PsiI TTATAA 1 cut(s) 1173
Psp6I CCWGG 2 cut(s) 900, 986
PspEI GGTNACC 1 cut(s) 998
PspGI CCWGG 2 cut(s) 900, 986
PspN4I GGNNCC 3 cut(s) 731, 958, 959
PspOMI GGGCCC 1 cut(s) 956
PspPI GGNCC 5 cut(s) 257, 956, 957, 1285, 1306
PspXI VCTCGAGB 1 cut(s) 866
PstI CTGCAG 1 cut(s) 523
PsuI RGATCY 1 cut(s) 729
PsyI GACNNNGTC 1 cut(s) 722
PvuII CAGCTG 1 cut(s) 325
RsaI GTAC 4 cut(s) 181, 314, 648, 1226
RsaNI GTAC 4 cut(s) 180, 313, 647, 1225
RseI CAYNNNNRTG 2 cut(s) 225, 1240
SaqAI TTAA 4 cut(s) 42, 303, 1008, 1064
SatI GCNGC 3 cut(s) 69, 326, 1129
Sau3AI GATC 5 cut(s) 33, 331, 427, 532, 729
Sau96I GGNCC 5 cut(s) 257, 956, 957, 1285, 1306
ScaI AGTACT 1 cut(s) 181
SchI GAGTC 3 cut(s) 204, 773, 825
ScrFI CCNGG 2 cut(s) 902, 988
SduI GDGCHC 3 cut(s) 27, 366, 960
SfaNI GCATC 3 cut(s) 140, 193, 803
SfcI CTRYAG 1 cut(s) 519
Sfr274I CTCGAG 1 cut(s) 866
SinI GGWCC 3 cut(s) 257, 1285, 1306
SlaI CTCGAG 1 cut(s) 866
SmiMI CAYNNNNRTG 2 cut(s) 225, 1240
SmlI CTYRAG 2 cut(s) 347, 866
SmoI CTYRAG 2 cut(s) 347, 866
Sse9I AATT 8 cut(s) 39, 252, 299, 356, 743, 810, 972, 1260
SsiI CCGC 2 cut(s) 496, 527
SspI AATATT 1 cut(s) 547
SspMI CTAG 5 cut(s) 156, 177, 240, 593, 1014
StyD4I CCNGG 2 cut(s) 900, 986
StyI CCWWGG 4 cut(s) 268, 291, 608, 936
TaaI ACNGT 7 cut(s) 473, 540, 626, 945, 1159, 1167, 1285
TaiI ACGT 1 cut(s) 648
TaqI TCGA 4 cut(s) 36, 95, 334, 867
TaqII GACCGA 1 cut(s) 1294
TasI AATT 8 cut(s) 39, 252, 299, 356, 743, 810, 972, 1260
TatI WGTACW 2 cut(s) 179, 1224
TfiI GAWTC 2 cut(s) 280, 950
Tru1I TTAA 4 cut(s) 42, 303, 1008, 1064
Tru9I TTAA 4 cut(s) 42, 303, 1008, 1064
TscAI CASTG 4 cut(s) 445, 543, 950, 1164
TseFI GTSAC 3 cut(s) 578, 665, 998
TseI GCWGC 3 cut(s) 68, 325, 1128
Tsp45I GTSAC 3 cut(s) 578, 665, 998
TspDTI ATGAA 2 cut(s) 669, 1043
TspRI CASTG 4 cut(s) 445, 543, 950, 1164
Tth111I GACNNNGTC 1 cut(s) 722
VpaK11BI GGWCC 3 cut(s) 257, 1285, 1306
XapI RAATTY 1 cut(s) 299
XcmI CCANNNNNNNNNTGG 1 cut(s) 95
XhoI CTCGAG 1 cut(s) 866
XmiI GTMKAC 1 cut(s) 1203
XspI CTAG 5 cut(s) 156, 177, 240, 593, 1014
ZrmI AGTACT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.