Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 2 cut(s) | 75, 164 |
| AccI | GTMKAC | 1 cut(s) | 126 |
| AcsI | RAATTY | 1 cut(s) | 262 |
| AcuI | CTGAAG | 1 cut(s) | 262 |
| AfaI | GTAC | 2 cut(s) | 271, 515 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 75, 164 |
| AgsI | TTSAA | 3 cut(s) | 245, 311, 510 |
| AjnI | CCWGG | 2 cut(s) | 194, 331 |
| AleI | CACNNNNGTG | 1 cut(s) | 137 |
| AluBI | AGCT | 4 cut(s) | 314, 364, 422, 485 |
| AluI | AGCT | 4 cut(s) | 314, 364, 422, 485 |
| Alw21I | GWGCWC | 1 cut(s) | 387 |
| Alw44I | GTGCAC | 1 cut(s) | 383 |
| AoxI | GGCC | 1 cut(s) | 83 |
| ApaLI | GTGCAC | 1 cut(s) | 383 |
| ApeKI | GCWGC | 1 cut(s) | 491 |
| ApoI | RAATTY | 1 cut(s) | 262 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 134, 166 |
| AspLEI | GCGC | 1 cut(s) | 416 |
| AspS9I | GGNCC | 2 cut(s) | 64, 211 |
| AvaII | GGWCC | 2 cut(s) | 64, 211 |
| BaeGI | GKGCMC | 1 cut(s) | 387 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 506, 538 |
| BauI | CACGAG | 1 cut(s) | 386 |
| Bbv12I | GWGCWC | 1 cut(s) | 387 |
| BbvI | GCAGC | 1 cut(s) | 503 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 106, 140 |
| BciT130I | CCWGG | 2 cut(s) | 196, 333 |
| BciVI | GTATCC | 1 cut(s) | 26 |
| BclI | TGATCA | 1 cut(s) | 351 |
| BfaI | CTAG | 6 cut(s) | 267, 371, 417, 423, 482, 547 |
| BfoI | RGCGCY | 1 cut(s) | 417 |
| BfuI | GTATCC | 1 cut(s) | 26 |
| BisI | GCNGC | 1 cut(s) | 492 |
| BlsI | GCNGC | 1 cut(s) | 493 |
| Bme1390I | CCNGG | 2 cut(s) | 196, 333 |
| Bme18I | GGWCC | 2 cut(s) | 64, 211 |
| BmgT120I | GGNCC | 2 cut(s) | 64, 211 |
| BmiI | GGNNCC | 4 cut(s) | 65, 66, 212, 213 |
| BmrFI | CCNGG | 2 cut(s) | 196, 333 |
| BsaJI | CCNNGG | 4 cut(s) | 177, 195, 331, 497 |
| BsaWI | WCCGGW | 1 cut(s) | 247 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 75, 164 |
| Bse3DI | GCAATG | 1 cut(s) | 401 |
| BseBI | CCWGG | 2 cut(s) | 196, 333 |
| BseDI | CCNNGG | 4 cut(s) | 177, 195, 331, 497 |
| BseGI | GGATG | 4 cut(s) | 94, 144, 232, 459 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 75, 164 |
| BseMI | GCAATG | 1 cut(s) | 401 |
| BseMII | CTCAG | 2 cut(s) | 135, 351 |
| BseSI | GKGCMC | 1 cut(s) | 387 |
| BseXI | GCAGC | 1 cut(s) | 503 |
| BshFI | GGCC | 1 cut(s) | 85 |
| BsiHKAI | GWGCWC | 1 cut(s) | 387 |
| BsiSI | CCGG | 2 cut(s) | 86, 248 |
| BslFI | GGGAC | 3 cut(s) | 77, 140, 224 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 75, 164 |
| BsmFI | GGGAC | 3 cut(s) | 77, 140, 224 |
| BsmI | GAATGC | 1 cut(s) | 449 |
| BsnI | GGCC | 1 cut(s) | 85 |
| Bsp1286I | GDGCHC | 1 cut(s) | 387 |
| Bsp143I | GATC | 1 cut(s) | 351 |
| Bsp19I | CCATGG | 2 cut(s) | 177, 497 |
| BspANI | GGCC | 1 cut(s) | 85 |
| BspCNI | CTCAG | 2 cut(s) | 134, 352 |
| BspLI | GGNNCC | 4 cut(s) | 65, 66, 212, 213 |
| BsrDI | GCAATG | 1 cut(s) | 401 |
| BssECI | CCNNGG | 4 cut(s) | 177, 195, 331, 497 |
| BssMI | GATC | 1 cut(s) | 351 |
| BssSI | CACGAG | 1 cut(s) | 386 |
| BssT1I | CCWWGG | 2 cut(s) | 177, 497 |
| Bst2BI | CACGAG | 1 cut(s) | 386 |
| Bst2UI | CCWGG | 2 cut(s) | 196, 333 |
| Bst4CI | ACNGT | 1 cut(s) | 14 |
| Bst6I | CTCTTC | 2 cut(s) | 483, 527 |
| BstC8I | GCNNGC | 1 cut(s) | 316 |
| BstDEI | CTNAG | 2 cut(s) | 121, 360 |
| BstDSI | CCRYGG | 2 cut(s) | 177, 497 |
| BstEII | GGTNACC | 1 cut(s) | 8 |
| BstF5I | GGATG | 4 cut(s) | 94, 144, 232, 459 |
| BstH2I | RGCGCY | 1 cut(s) | 417 |
| BstHHI | GCGC | 1 cut(s) | 416 |
| BstKTI | GATC | 1 cut(s) | 354 |
| BstMBI | GATC | 1 cut(s) | 351 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 491 |
| BstNI | CCWGG | 2 cut(s) | 196, 333 |
| BstPI | GGTNACC | 1 cut(s) | 8 |
| BstSCI | CCNGG | 2 cut(s) | 194, 331 |
| BstSLI | GKGCMC | 1 cut(s) | 387 |
| BstV1I | GCAGC | 1 cut(s) | 503 |
| BsuI | GTATCC | 1 cut(s) | 26 |
| BsuRI | GGCC | 1 cut(s) | 85 |
| BtgI | CCRYGG | 2 cut(s) | 177, 497 |
| BtsCI | GGATG | 4 cut(s) | 94, 144, 232, 459 |
| Cac8I | GCNNGC | 1 cut(s) | 316 |
| CfoI | GCGC | 1 cut(s) | 416 |
| Cfr13I | GGNCC | 2 cut(s) | 64, 211 |
| Csp6I | GTAC | 2 cut(s) | 270, 514 |
| CviAII | CATG | 2 cut(s) | 178, 498 |
| CviJI | RGCY | 5 cut(s) | 85, 314, 364, 422, 485 |
| CviKI_1 | RGCY | 5 cut(s) | 85, 314, 364, 422, 485 |
| CviQI | GTAC | 2 cut(s) | 270, 514 |
| DdeI | CTNAG | 2 cut(s) | 121, 360 |
| DpnI | GATC | 1 cut(s) | 353 |
| DpnII | GATC | 1 cut(s) | 351 |
| Eam1104I | CTCTTC | 2 cut(s) | 483, 527 |
| EarI | CTCTTC | 2 cut(s) | 483, 527 |
| Eco130I | CCWWGG | 2 cut(s) | 177, 497 |
| Eco47I | GGWCC | 2 cut(s) | 64, 211 |
| Eco57I | CTGAAG | 1 cut(s) | 262 |
| Eco91I | GGTNACC | 1 cut(s) | 8 |
| EcoO109I | RGGNCCY | 2 cut(s) | 64, 211 |
| EcoO65I | GGTNACC | 1 cut(s) | 8 |
| EcoRI | GAATTC | 1 cut(s) | 262 |
| EcoRII | CCWGG | 2 cut(s) | 194, 331 |
| EcoT14I | CCWWGG | 2 cut(s) | 177, 497 |
| EcoT22I | ATGCAT | 1 cut(s) | 49 |
| ErhI | CCWWGG | 2 cut(s) | 177, 497 |
| FaeI | CATG | 2 cut(s) | 181, 501 |
| FaiI | YATR | 9 cut(s) | 179, 185, 260, 408, 431, 488, 499, 531, 539 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 199, 231 |
| FaqI | GGGAC | 3 cut(s) | 77, 140, 224 |
| FatI | CATG | 2 cut(s) | 177, 497 |
| FbaI | TGATCA | 1 cut(s) | 351 |
| FblI | GTMKAC | 1 cut(s) | 126 |
| Fnu4HI | GCNGC | 1 cut(s) | 492 |
| FokI | GGATG | 4 cut(s) | 101, 131, 239, 446 |
| Fsp4HI | GCNGC | 1 cut(s) | 492 |
| FspBI | CTAG | 6 cut(s) | 267, 371, 417, 423, 482, 547 |
| GlaI | GCGC | 1 cut(s) | 415 |
| GluI | GCNGC | 1 cut(s) | 492 |
| HaeII | RGCGCY | 1 cut(s) | 417 |
| HaeIII | GGCC | 1 cut(s) | 85 |
| HapII | CCGG | 2 cut(s) | 86, 248 |
| HhaI | GCGC | 1 cut(s) | 416 |
| Hin1II | CATG | 2 cut(s) | 181, 501 |
| Hin6I | GCGC | 1 cut(s) | 414 |
| HinP1I | GCGC | 1 cut(s) | 414 |
| HincII | GTYRAC | 1 cut(s) | 127 |
| HindII | GTYRAC | 1 cut(s) | 127 |
| HinfI | GANTC | 1 cut(s) | 336 |
| HpaII | CCGG | 2 cut(s) | 86, 248 |
| Hpy166II | GTNNAC | 3 cut(s) | 127, 142, 385 |
| Hpy188I | TCNGA | 1 cut(s) | 281 |
| Hpy8I | GTNNAC | 3 cut(s) | 127, 142, 385 |
| HpyAV | CCTTC | 2 cut(s) | 201, 225 |
| HpyCH4III | ACNGT | 1 cut(s) | 14 |
| HpyCH4V | TGCA | 5 cut(s) | 47, 170, 385, 406, 447 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 491 |
| HpyF3I | CTNAG | 2 cut(s) | 121, 360 |
| Hsp92II | CATG | 2 cut(s) | 181, 501 |
| HspAI | GCGC | 1 cut(s) | 414 |
| KflI | GGGWCCC | 2 cut(s) | 64, 211 |
| Ksp22I | TGATCA | 1 cut(s) | 351 |
| Kzo9I | GATC | 1 cut(s) | 351 |
| Lsp1109I | GCAGC | 1 cut(s) | 503 |
| MaeI | CTAG | 6 cut(s) | 267, 371, 417, 423, 482, 547 |
| MaeIII | GTNAC | 2 cut(s) | 8, 540 |
| MalI | GATC | 1 cut(s) | 353 |
| MboI | GATC | 1 cut(s) | 351 |
| MboII | GAAGA | 3 cut(s) | 470, 498, 514 |
| MhlI | GDGCHC | 1 cut(s) | 387 |
| MluCI | AATT | 3 cut(s) | 149, 201, 262 |
| MnlI | CCTC | 3 cut(s) | 107, 275, 486 |
| Mph1103I | ATGCAT | 1 cut(s) | 49 |
| MslI | CAYNNNNRTG | 2 cut(s) | 137, 468 |
| MspI | CCGG | 2 cut(s) | 86, 248 |
| MspR9I | CCNGG | 2 cut(s) | 196, 333 |
| Mva1269I | GAATGC | 1 cut(s) | 449 |
| MvaI | CCWGG | 2 cut(s) | 196, 333 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 491 |
| NcoI | CCATGG | 2 cut(s) | 177, 497 |
| NdeII | GATC | 1 cut(s) | 351 |
| NlaIII | CATG | 2 cut(s) | 181, 501 |
| NlaIV | GGNNCC | 4 cut(s) | 65, 66, 212, 213 |
| NsiI | ATGCAT | 1 cut(s) | 49 |
| OliI | CACNNNNGTG | 1 cut(s) | 137 |
| PctI | GAATGC | 1 cut(s) | 449 |
| PfeI | GAWTC | 1 cut(s) | 336 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 75, 164 |
| PkrI | GCNGC | 1 cut(s) | 493 |
| PpuMI | RGGWCCY | 2 cut(s) | 64, 211 |
| Psp5II | RGGWCCY | 2 cut(s) | 64, 211 |
| Psp6I | CCWGG | 2 cut(s) | 194, 331 |
| PspEI | GGTNACC | 1 cut(s) | 8 |
| PspGI | CCWGG | 2 cut(s) | 194, 331 |
| PspN4I | GGNNCC | 4 cut(s) | 65, 66, 212, 213 |
| PspPI | GGNCC | 2 cut(s) | 64, 211 |
| PspPPI | RGGWCCY | 2 cut(s) | 64, 211 |
| RsaI | GTAC | 2 cut(s) | 271, 515 |
| RsaNI | GTAC | 2 cut(s) | 270, 514 |
| RseI | CAYNNNNRTG | 2 cut(s) | 137, 468 |
| SalI | GTCGAC | 1 cut(s) | 125 |
| SatI | GCNGC | 1 cut(s) | 492 |
| Sau3AI | GATC | 1 cut(s) | 351 |
| Sau96I | GGNCC | 2 cut(s) | 64, 211 |
| ScrFI | CCNGG | 2 cut(s) | 196, 333 |
| SduI | GDGCHC | 1 cut(s) | 387 |
| SetI | ASST | 6 cut(s) | 10, 316, 328, 366, 424, 487 |
| SinI | GGWCC | 2 cut(s) | 64, 211 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 137, 468 |
| Sse9I | AATT | 3 cut(s) | 149, 201, 262 |
| SspMI | CTAG | 6 cut(s) | 267, 371, 417, 423, 482, 547 |
| StyD4I | CCNGG | 2 cut(s) | 194, 331 |
| StyI | CCWWGG | 2 cut(s) | 177, 497 |
| TaaI | ACNGT | 1 cut(s) | 14 |
| TaqI | TCGA | 2 cut(s) | 126, 347 |
| TasI | AATT | 3 cut(s) | 149, 201, 262 |
| TatI | WGTACW | 1 cut(s) | 513 |
| TfiI | GAWTC | 1 cut(s) | 336 |
| TseI | GCWGC | 1 cut(s) | 491 |
| TspDTI | ATGAA | 4 cut(s) | 17, 243, 275, 446 |
| TspGWI | ACGGA | 1 cut(s) | 287 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 75, 164 |
| VneI | GTGCAC | 1 cut(s) | 383 |
| VpaK11BI | GGWCC | 2 cut(s) | 64, 211 |
| XapI | RAATTY | 1 cut(s) | 262 |
| XmiI | GTMKAC | 1 cut(s) | 126 |
| XspI | CTAG | 6 cut(s) | 267, 371, 417, 423, 482, 547 |
| Zsp2I | ATGCAT | 1 cut(s) | 49 |