Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 331 |
| Acc36I | ACCTGC | 1 cut(s) | 331 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 22 |
| AccI | GTMKAC | 1 cut(s) | 226 |
| AccII | CGCG | 2 cut(s) | 382, 612 |
| AciI | CCGC | 1 cut(s) | 586 |
| AclWI | GGATC | 1 cut(s) | 647 |
| AfaI | GTAC | 2 cut(s) | 140, 235 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 22, 174, 323, 636, 643 |
| AflII | CTTAAG | 1 cut(s) | 572 |
| AflIII | ACRYGT | 1 cut(s) | 380 |
| AgsI | TTSAA | 4 cut(s) | 203, 434, 470, 485 |
| AluBI | AGCT | 3 cut(s) | 20, 110, 442 |
| AluI | AGCT | 3 cut(s) | 20, 110, 442 |
| Alw26I | GTCTC | 1 cut(s) | 403 |
| AlwI | GGATC | 1 cut(s) | 647 |
| ApeKI | GCWGC | 3 cut(s) | 268, 271, 337 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 83 |
| AspLEI | GCGC | 2 cut(s) | 99, 614 |
| AspS9I | GGNCC | 2 cut(s) | 4, 328 |
| AsuC2I | CCSGG | 1 cut(s) | 631 |
| AsuHPI | GGTGA | 1 cut(s) | 593 |
| AvaII | GGWCC | 2 cut(s) | 4, 328 |
| BbvI | GCAGC | 3 cut(s) | 255, 283, 324 |
| BccI | CCATC | 2 cut(s) | 200, 656 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 329, 363 |
| BcnI | CCSGG | 1 cut(s) | 631 |
| BcoDI | GTCTC | 1 cut(s) | 403 |
| BfaI | CTAG | 1 cut(s) | 150 |
| BfmI | CTRYAG | 3 cut(s) | 269, 321, 338 |
| BfoI | RGCGCY | 1 cut(s) | 100 |
| BfrI | CTTAAG | 1 cut(s) | 572 |
| BfuAI | ACCTGC | 1 cut(s) | 331 |
| BisI | GCNGC | 3 cut(s) | 269, 272, 338 |
| BlsI | GCNGC | 3 cut(s) | 270, 273, 339 |
| Bme1390I | CCNGG | 1 cut(s) | 631 |
| Bme18I | GGWCC | 2 cut(s) | 4, 328 |
| BmgT120I | GGNCC | 2 cut(s) | 4, 328 |
| BmiI | GGNNCC | 3 cut(s) | 329, 330, 635 |
| BmrFI | CCNGG | 1 cut(s) | 631 |
| BmsI | GCATC | 2 cut(s) | 300, 411 |
| BoxI | GACNNNNGTC | 1 cut(s) | 186 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 509, 541 |
| BpmI | CTGGAG | 3 cut(s) | 414, 464, 565 |
| BpuMI | CCSGG | 1 cut(s) | 631 |
| Bsa29I | ATCGAT | 1 cut(s) | 82 |
| BsaAI | YACGTR | 1 cut(s) | 72 |
| BsaWI | WCCGGW | 1 cut(s) | 636 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 22, 174, 323, 636, 643 |
| Bse1I | ACTGG | 1 cut(s) | 548 |
| BseCI | ATCGAT | 1 cut(s) | 82 |
| BseGI | GGATG | 3 cut(s) | 192, 315, 664 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 22, 174, 323, 636, 643 |
| BseMII | CTCAG | 1 cut(s) | 184 |
| BseNI | ACTGG | 1 cut(s) | 548 |
| BseRI | GAGGAG | 1 cut(s) | 272 |
| BseXI | GCAGC | 3 cut(s) | 255, 283, 324 |
| Bsh1236I | CGCG | 2 cut(s) | 382, 612 |
| BshVI | ATCGAT | 1 cut(s) | 82 |
| BsiSI | CCGG | 2 cut(s) | 631, 637 |
| BslFI | GGGAC | 2 cut(s) | 174, 341 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 22, 174, 323, 636, 643 |
| BsmAI | GTCTC | 1 cut(s) | 403 |
| BsmFI | GGGAC | 2 cut(s) | 174, 341 |
| BsmI | GAATGC | 1 cut(s) | 388 |
| Bsp143I | GATC | 1 cut(s) | 639 |
| BspACI | CCGC | 1 cut(s) | 586 |
| BspCNI | CTCAG | 1 cut(s) | 183 |
| BspDI | ATCGAT | 1 cut(s) | 82 |
| BspFNI | CGCG | 2 cut(s) | 382, 612 |
| BspLI | GGNNCC | 3 cut(s) | 329, 330, 635 |
| BspMAI | CTGCAG | 2 cut(s) | 273, 342 |
| BspMI | ACCTGC | 1 cut(s) | 331 |
| BspPI | GGATC | 1 cut(s) | 647 |
| BspTI | CTTAAG | 1 cut(s) | 572 |
| BsrI | ACTGG | 1 cut(s) | 548 |
| BssMI | GATC | 1 cut(s) | 639 |
| Bst4CI | ACNGT | 3 cut(s) | 8, 32, 504 |
| Bst6I | CTCTTC | 1 cut(s) | 168 |
| BstAFI | CTTAAG | 1 cut(s) | 572 |
| BstBAI | YACGTR | 1 cut(s) | 72 |
| BstC8I | GCNNGC | 1 cut(s) | 112 |
| BstDEI | CTNAG | 1 cut(s) | 170 |
| BstEII | GGTNACC | 1 cut(s) | 581 |
| BstF5I | GGATG | 3 cut(s) | 192, 315, 664 |
| BstFNI | CGCG | 2 cut(s) | 382, 612 |
| BstH2I | RGCGCY | 1 cut(s) | 100 |
| BstHHI | GCGC | 2 cut(s) | 99, 614 |
| BstKTI | GATC | 1 cut(s) | 642 |
| BstMAI | GTCTC | 1 cut(s) | 403 |
| BstMBI | GATC | 1 cut(s) | 639 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 186 |
| BstPI | GGTNACC | 1 cut(s) | 581 |
| BstSCI | CCNGG | 1 cut(s) | 629 |
| BstSFI | CTRYAG | 3 cut(s) | 269, 321, 338 |
| BstUI | CGCG | 2 cut(s) | 382, 612 |
| BstV1I | GCAGC | 3 cut(s) | 255, 283, 324 |
| Bsu15I | ATCGAT | 1 cut(s) | 82 |
| BsuTUI | ATCGAT | 1 cut(s) | 82 |
| BtsCI | GGATG | 3 cut(s) | 192, 315, 664 |
| BtsIMutI | CAGTG | 1 cut(s) | 572 |
| BveI | ACCTGC | 1 cut(s) | 331 |
| Cac8I | GCNNGC | 1 cut(s) | 112 |
| CfoI | GCGC | 2 cut(s) | 99, 614 |
| Cfr13I | GGNCC | 2 cut(s) | 4, 328 |
| ClaI | ATCGAT | 1 cut(s) | 82 |
| CseI | GACGC | 1 cut(s) | 618 |
| Csp6I | GTAC | 2 cut(s) | 139, 234 |
| CviAII | CATG | 2 cut(s) | 275, 334 |
| CviJI | RGCY | 7 cut(s) | 20, 110, 268, 442, 497, 571, 629 |
| CviKI_1 | RGCY | 7 cut(s) | 20, 110, 268, 442, 497, 571, 629 |
| CviQI | GTAC | 2 cut(s) | 139, 234 |
| DdeI | CTNAG | 1 cut(s) | 170 |
| DpnI | GATC | 1 cut(s) | 641 |
| DpnII | GATC | 1 cut(s) | 639 |
| Eam1104I | CTCTTC | 1 cut(s) | 168 |
| EarI | CTCTTC | 1 cut(s) | 168 |
| Eco47I | GGWCC | 2 cut(s) | 4, 328 |
| Eco91I | GGTNACC | 1 cut(s) | 581 |
| EcoO109I | RGGNCCY | 1 cut(s) | 328 |
| EcoO65I | GGTNACC | 1 cut(s) | 581 |
| FaeI | CATG | 2 cut(s) | 278, 337 |
| FaiI | YATR | 6 cut(s) | 47, 56, 127, 276, 323, 335 |
| FaqI | GGGAC | 2 cut(s) | 174, 341 |
| FatI | CATG | 2 cut(s) | 274, 333 |
| FblI | GTMKAC | 1 cut(s) | 226 |
| Fnu4HI | GCNGC | 3 cut(s) | 269, 272, 338 |
| FokI | GGATG | 2 cut(s) | 179, 322 |
| Fsp4HI | GCNGC | 3 cut(s) | 269, 272, 338 |
| FspBI | CTAG | 1 cut(s) | 150 |
| GlaI | GCGC | 2 cut(s) | 98, 613 |
| GluI | GCNGC | 3 cut(s) | 269, 272, 338 |
| GsuI | CTGGAG | 3 cut(s) | 414, 464, 565 |
| HaeII | RGCGCY | 1 cut(s) | 100 |
| HapII | CCGG | 2 cut(s) | 631, 637 |
| HgaI | GACGC | 1 cut(s) | 618 |
| HhaI | GCGC | 2 cut(s) | 99, 614 |
| Hin1II | CATG | 2 cut(s) | 278, 337 |
| Hin6I | GCGC | 2 cut(s) | 97, 612 |
| HinP1I | GCGC | 2 cut(s) | 97, 612 |
| HincII | GTYRAC | 3 cut(s) | 28, 227, 371 |
| HindII | GTYRAC | 3 cut(s) | 28, 227, 371 |
| HindIII | AAGCTT | 1 cut(s) | 108 |
| HinfI | GANTC | 5 cut(s) | 51, 79, 84, 430, 475 |
| HpaII | CCGG | 2 cut(s) | 631, 637 |
| HphI | GGTGA | 1 cut(s) | 593 |
| Hpy166II | GTNNAC | 3 cut(s) | 28, 227, 371 |
| Hpy188I | TCNGA | 2 cut(s) | 181, 453 |
| Hpy188III | TCNNGA | 1 cut(s) | 393 |
| Hpy8I | GTNNAC | 3 cut(s) | 28, 227, 371 |
| HpyAV | CCTTC | 2 cut(s) | 607, 649 |
| HpyCH4III | ACNGT | 3 cut(s) | 8, 32, 504 |
| HpyCH4IV | ACGT | 1 cut(s) | 71 |
| HpyCH4V | TGCA | 4 cut(s) | 271, 340, 490, 530 |
| HpyF3I | CTNAG | 1 cut(s) | 170 |
| HpySE526I | ACGT | 1 cut(s) | 71 |
| Hsp92II | CATG | 2 cut(s) | 278, 337 |
| HspAI | GCGC | 2 cut(s) | 97, 612 |
| KflI | GGGWCCC | 1 cut(s) | 328 |
| Kzo9I | GATC | 1 cut(s) | 639 |
| LmnI | GCTCC | 1 cut(s) | 94 |
| Lsp1109I | GCAGC | 3 cut(s) | 255, 283, 324 |
| LweI | GCATC | 2 cut(s) | 300, 411 |
| MaeI | CTAG | 1 cut(s) | 150 |
| MaeII | ACGT | 1 cut(s) | 71 |
| MaeIII | GTNAC | 4 cut(s) | 205, 519, 555, 581 |
| MalI | GATC | 1 cut(s) | 641 |
| MboI | GATC | 1 cut(s) | 639 |
| MboII | GAAGA | 3 cut(s) | 155, 290, 592 |
| MluCI | AATT | 1 cut(s) | 245 |
| MluI | ACGCGT | 1 cut(s) | 380 |
| MlyI | GAGTC | 1 cut(s) | 484 |
| MmeI | TCCRAC | 4 cut(s) | 287, 322, 341, 427 |
| MnlI | CCTC | 8 cut(s) | 179, 206, 208, 250, 439, 540, 546, 571 |
| MroXI | GAANNNNTTC | 1 cut(s) | 83 |
| MseI | TTAA | 1 cut(s) | 573 |
| MspCI | CTTAAG | 1 cut(s) | 572 |
| MspI | CCGG | 2 cut(s) | 631, 637 |
| MspR9I | CCNGG | 1 cut(s) | 631 |
| Mva1269I | GAATGC | 1 cut(s) | 388 |
| MvnI | CGCG | 2 cut(s) | 382, 612 |
| NciI | CCSGG | 1 cut(s) | 631 |
| NdeII | GATC | 1 cut(s) | 639 |
| NlaIII | CATG | 2 cut(s) | 278, 337 |
| NlaIV | GGNNCC | 3 cut(s) | 329, 330, 635 |
| NmuCI | GTSAC | 2 cut(s) | 555, 581 |
| PaqCI | CACCTGC | 1 cut(s) | 331 |
| PctI | GAATGC | 1 cut(s) | 388 |
| PdmI | GAANNNNTTC | 1 cut(s) | 83 |
| PfeI | GAWTC | 4 cut(s) | 51, 79, 84, 430 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 22 |
| PkrI | GCNGC | 3 cut(s) | 270, 273, 339 |
| PleI | GAGTC | 1 cut(s) | 483 |
| PpsI | GAGTC | 1 cut(s) | 483 |
| Ppu21I | YACGTR | 1 cut(s) | 72 |
| PpuMI | RGGWCCY | 1 cut(s) | 328 |
| PshAI | GACNNNNGTC | 1 cut(s) | 186 |
| Psp5II | RGGWCCY | 1 cut(s) | 328 |
| PspEI | GGTNACC | 1 cut(s) | 581 |
| PspN4I | GGNNCC | 3 cut(s) | 329, 330, 635 |
| PspPI | GGNCC | 2 cut(s) | 4, 328 |
| PspPPI | RGGWCCY | 1 cut(s) | 328 |
| PstI | CTGCAG | 2 cut(s) | 273, 342 |
| RsaI | GTAC | 2 cut(s) | 140, 235 |
| RsaNI | GTAC | 2 cut(s) | 139, 234 |
| SalI | GTCGAC | 1 cut(s) | 225 |
| SaqAI | TTAA | 1 cut(s) | 573 |
| SatI | GCNGC | 3 cut(s) | 269, 272, 338 |
| Sau3AI | GATC | 1 cut(s) | 639 |
| Sau96I | GGNCC | 2 cut(s) | 4, 328 |
| SchI | GAGTC | 1 cut(s) | 484 |
| ScrFI | CCNGG | 1 cut(s) | 631 |
| SfaNI | GCATC | 2 cut(s) | 300, 411 |
| SfcI | CTRYAG | 3 cut(s) | 269, 321, 338 |
| SinI | GGWCC | 2 cut(s) | 4, 328 |
| SmlI | CTYRAG | 1 cut(s) | 572 |
| SmoI | CTYRAG | 1 cut(s) | 572 |
| Sse9I | AATT | 1 cut(s) | 245 |
| SsiI | CCGC | 1 cut(s) | 586 |
| SspMI | CTAG | 1 cut(s) | 150 |
| StyD4I | CCNGG | 1 cut(s) | 629 |
| TaaI | ACNGT | 3 cut(s) | 8, 32, 504 |
| TaiI | ACGT | 1 cut(s) | 74 |
| TaqI | TCGA | 5 cut(s) | 42, 77, 82, 226, 349 |
| TasI | AATT | 1 cut(s) | 245 |
| TfiI | GAWTC | 4 cut(s) | 51, 79, 84, 430 |
| Tru1I | TTAA | 1 cut(s) | 573 |
| Tru9I | TTAA | 1 cut(s) | 573 |
| TscAI | CASTG | 1 cut(s) | 572 |
| TseFI | GTSAC | 2 cut(s) | 555, 581 |
| TseI | GCWGC | 3 cut(s) | 268, 271, 337 |
| Tsp45I | GTSAC | 2 cut(s) | 555, 581 |
| TspDTI | ATGAA | 4 cut(s) | 43, 291, 470, 549 |
| TspRI | CASTG | 1 cut(s) | 572 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 22 |
| Vha464I | CTTAAG | 1 cut(s) | 572 |
| VpaK11BI | GGWCC | 2 cut(s) | 4, 328 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 340 |
| XmiI | GTMKAC | 1 cut(s) | 226 |
| XmnI | GAANNNNTTC | 1 cut(s) | 83 |
| XspI | CTAG | 1 cut(s) | 150 |