Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 439 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 316 |
| AciI | CCGC | 8 cut(s) | 148, 352, 357, 443, 467, 533, 602, 776 |
| AclWI | GGATC | 2 cut(s) | 709, 934 |
| AcoI | YGGCCR | 2 cut(s) | 307, 318 |
| AcsI | RAATTY | 2 cut(s) | 384, 637 |
| AcuI | CTGAAG | 2 cut(s) | 277, 692 |
| AcyI | GRCGYC | 2 cut(s) | 231, 440 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 227, 316 |
| AgsI | TTSAA | 2 cut(s) | 484, 916 |
| AjnI | CCWGG | 3 cut(s) | 197, 315, 475 |
| AluBI | AGCT | 9 cut(s) | 156, 238, 282, 297, 591, 627, 683, 809, 958 |
| AluI | AGCT | 9 cut(s) | 156, 238, 282, 297, 591, 627, 683, 809, 958 |
| Alw21I | GWGCWC | 2 cut(s) | 436, 685 |
| Alw26I | GTCTC | 2 cut(s) | 127, 370 |
| AlwI | GGATC | 2 cut(s) | 709, 934 |
| AoxI | GGCC | 4 cut(s) | 307, 318, 473, 738 |
| ApeKI | GCWGC | 3 cut(s) | 279, 627, 950 |
| ApoI | RAATTY | 2 cut(s) | 384, 637 |
| AspLEI | GCGC | 4 cut(s) | 442, 632, 652, 781 |
| AspS9I | GGNCC | 1 cut(s) | 201 |
| AsuC2I | CCSGG | 1 cut(s) | 267 |
| AsuHPI | GGTGA | 6 cut(s) | 40, 71, 235, 526, 572, 667 |
| AvaII | GGWCC | 1 cut(s) | 201 |
| BaeGI | GKGCMC | 1 cut(s) | 460 |
| BalI | TGGCCA | 1 cut(s) | 320 |
| BanI | GGYRCC | 1 cut(s) | 439 |
| BanII | GRGCYC | 1 cut(s) | 685 |
| BbsI | GAAGAC | 1 cut(s) | 34 |
| Bbv12I | GWGCWC | 2 cut(s) | 436, 685 |
| BbvI | GCAGC | 3 cut(s) | 291, 614, 937 |
| BccI | CCATC | 2 cut(s) | 557, 604 |
| BceAI | ACGGC | 3 cut(s) | 146, 292, 346 |
| BciT130I | CCWGG | 3 cut(s) | 199, 317, 477 |
| BclI | TGATCA | 2 cut(s) | 450, 492 |
| BcnI | CCSGG | 1 cut(s) | 267 |
| BcoDI | GTCTC | 2 cut(s) | 127, 370 |
| BfmI | CTRYAG | 1 cut(s) | 930 |
| BfoI | RGCGCY | 3 cut(s) | 443, 653, 782 |
| BisI | GCNGC | 6 cut(s) | 280, 443, 468, 628, 777, 951 |
| BlsI | GCNGC | 6 cut(s) | 281, 444, 469, 629, 778, 952 |
| Bme1390I | CCNGG | 4 cut(s) | 199, 267, 317, 477 |
| Bme18I | GGWCC | 1 cut(s) | 201 |
| BmgT120I | GGNCC | 1 cut(s) | 201 |
| BmiI | GGNNCC | 2 cut(s) | 427, 441 |
| BmrFI | CCNGG | 4 cut(s) | 199, 267, 317, 477 |
| BmrI | ACTGGG | 1 cut(s) | 32 |
| BmsI | GCATC | 1 cut(s) | 840 |
| BmuI | ACTGGG | 1 cut(s) | 32 |
| BpiI | GAAGAC | 1 cut(s) | 34 |
| BpmI | CTGGAG | 1 cut(s) | 376 |
| BpuMI | CCSGG | 1 cut(s) | 267 |
| BsaHI | GRCGYC | 2 cut(s) | 231, 440 |
| BsaJI | CCNNGG | 4 cut(s) | 226, 274, 315, 324 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 877, 907 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 227, 316 |
| Bse118I | RCCGGY | 1 cut(s) | 428 |
| Bse1I | ACTGG | 1 cut(s) | 38 |
| Bse3DI | GCAATG | 1 cut(s) | 589 |
| BseBI | CCWGG | 3 cut(s) | 199, 317, 477 |
| BseDI | CCNNGG | 4 cut(s) | 226, 274, 315, 324 |
| BseGI | GGATG | 3 cut(s) | 601, 844, 855 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 227, 316 |
| BseMI | GCAATG | 1 cut(s) | 589 |
| BseNI | ACTGG | 1 cut(s) | 38 |
| BseSI | GKGCMC | 1 cut(s) | 460 |
| BseXI | GCAGC | 3 cut(s) | 291, 614, 937 |
| BseYI | CCCAGC | 2 cut(s) | 791, 881 |
| BsgI | GTGCAG | 2 cut(s) | 915, 936 |
| BshFI | GGCC | 4 cut(s) | 309, 320, 475, 740 |
| BshNI | GGYRCC | 1 cut(s) | 439 |
| BsiHKAI | GWGCWC | 2 cut(s) | 436, 685 |
| BsiSI | CCGG | 4 cut(s) | 267, 306, 369, 429 |
| BslFI | GGGAC | 1 cut(s) | 629 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 227, 316 |
| BsmAI | GTCTC | 2 cut(s) | 127, 370 |
| BsmBI | CGTCTC | 2 cut(s) | 127, 370 |
| BsmFI | GGGAC | 1 cut(s) | 629 |
| BsmI | GAATGC | 1 cut(s) | 712 |
| BsnI | GGCC | 4 cut(s) | 309, 320, 475, 740 |
| Bsp1286I | GDGCHC | 3 cut(s) | 436, 460, 685 |
| Bsp143I | GATC | 7 cut(s) | 420, 450, 492, 701, 825, 864, 926 |
| Bsp19I | CCATGG | 1 cut(s) | 324 |
| BspACI | CCGC | 8 cut(s) | 148, 352, 357, 443, 467, 533, 602, 776 |
| BspANI | GGCC | 4 cut(s) | 309, 320, 475, 740 |
| BspHI | TCATGA | 1 cut(s) | 417 |
| BspLI | GGNNCC | 2 cut(s) | 427, 441 |
| BspMAI | CTGCAG | 1 cut(s) | 934 |
| BspPI | GGATC | 2 cut(s) | 709, 934 |
| BspT107I | GGYRCC | 1 cut(s) | 439 |
| BsrDI | GCAATG | 1 cut(s) | 589 |
| BsrFI | RCCGGY | 1 cut(s) | 428 |
| BsrI | ACTGG | 1 cut(s) | 38 |
| BssAI | RCCGGY | 1 cut(s) | 428 |
| BssECI | CCNNGG | 4 cut(s) | 226, 274, 315, 324 |
| BssMI | GATC | 7 cut(s) | 420, 450, 492, 701, 825, 864, 926 |
| BssNI | GRCGYC | 2 cut(s) | 231, 440 |
| BssT1I | CCWWGG | 1 cut(s) | 324 |
| Bst2UI | CCWGG | 3 cut(s) | 199, 317, 477 |
| Bst4CI | ACNGT | 3 cut(s) | 121, 404, 910 |
| Bst6I | CTCTTC | 3 cut(s) | 112, 296, 479 |
| BstACI | GRCGYC | 2 cut(s) | 231, 440 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 360, 464 |
| BstC8I | GCNNGC | 1 cut(s) | 361 |
| BstDSI | CCRYGG | 2 cut(s) | 274, 324 |
| BstF5I | GGATG | 3 cut(s) | 601, 844, 855 |
| BstH2I | RGCGCY | 3 cut(s) | 443, 653, 782 |
| BstHHI | GCGC | 4 cut(s) | 442, 632, 652, 781 |
| BstKTI | GATC | 7 cut(s) | 423, 453, 495, 704, 828, 867, 929 |
| BstMAI | GTCTC | 2 cut(s) | 127, 370 |
| BstMBI | GATC | 7 cut(s) | 420, 450, 492, 701, 825, 864, 926 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 360, 369, 464, 588 |
| BstNI | CCWGG | 3 cut(s) | 199, 317, 477 |
| BstSCI | CCNGG | 4 cut(s) | 197, 265, 315, 475 |
| BstSFI | CTRYAG | 1 cut(s) | 930 |
| BstSLI | GKGCMC | 1 cut(s) | 460 |
| BstV1I | GCAGC | 3 cut(s) | 291, 614, 937 |
| BstV2I | GAAGAC | 1 cut(s) | 34 |
| BstX2I | RGATCY | 1 cut(s) | 926 |
| BstYI | RGATCY | 1 cut(s) | 926 |
| BsuRI | GGCC | 4 cut(s) | 309, 320, 475, 740 |
| BtgI | CCRYGG | 2 cut(s) | 274, 324 |
| BtgZI | GCGATG | 1 cut(s) | 623 |
| BtsCI | GGATG | 3 cut(s) | 601, 844, 855 |
| BtsI | GCAGTG | 1 cut(s) | 333 |
| BtsIMutI | CAGTG | 2 cut(s) | 333, 409 |
| Cac8I | GCNNGC | 1 cut(s) | 361 |
| CciI | TCATGA | 1 cut(s) | 417 |
| CfoI | GCGC | 4 cut(s) | 442, 632, 652, 781 |
| Cfr10I | RCCGGY | 1 cut(s) | 428 |
| Cfr13I | GGNCC | 1 cut(s) | 201 |
| CseI | GACGC | 2 cut(s) | 220, 352 |
| CviAII | CATG | 4 cut(s) | 190, 325, 418, 976 |
| DinI | GGCGCC | 1 cut(s) | 441 |
| DpnI | GATC | 7 cut(s) | 422, 452, 494, 703, 827, 866, 928 |
| DpnII | GATC | 7 cut(s) | 420, 450, 492, 701, 825, 864, 926 |
| EaeI | YGGCCR | 2 cut(s) | 307, 318 |
| Eam1104I | CTCTTC | 3 cut(s) | 112, 296, 479 |
| EarI | CTCTTC | 3 cut(s) | 112, 296, 479 |
| EciI | GGCGGA | 2 cut(s) | 137, 548 |
| Ecl136II | GAGCTC | 1 cut(s) | 683 |
| Eco130I | CCWWGG | 1 cut(s) | 324 |
| Eco147I | AGGCCT | 1 cut(s) | 475 |
| Eco24I | GRGCYC | 1 cut(s) | 685 |
| Eco47I | GGWCC | 1 cut(s) | 201 |
| Eco53kI | GAGCTC | 1 cut(s) | 683 |
| Eco57I | CTGAAG | 2 cut(s) | 277, 692 |
| EcoICRI | GAGCTC | 1 cut(s) | 683 |
| EcoRI | GAATTC | 2 cut(s) | 384, 637 |
| EcoRII | CCWGG | 3 cut(s) | 197, 315, 475 |
| EcoT14I | CCWWGG | 1 cut(s) | 324 |
| EcoT38I | GRGCYC | 1 cut(s) | 685 |
| EgeI | GGCGCC | 1 cut(s) | 441 |
| EheI | GGCGCC | 1 cut(s) | 441 |
| ErhI | CCWWGG | 1 cut(s) | 324 |
| Esp3I | CGTCTC | 2 cut(s) | 127, 370 |
| FaeI | CATG | 4 cut(s) | 193, 328, 421, 979 |
| FaiI | YATR | 8 cut(s) | 191, 326, 419, 497, 499, 743, 966, 977 |
| FaqI | GGGAC | 1 cut(s) | 629 |
| FatI | CATG | 4 cut(s) | 189, 324, 417, 975 |
| FauNDI | CATATG | 1 cut(s) | 497 |
| FbaI | TGATCA | 2 cut(s) | 450, 492 |
| Fnu4HI | GCNGC | 6 cut(s) | 280, 443, 468, 628, 777, 951 |
| FokI | GGATG | 3 cut(s) | 608, 851, 862 |
| FriOI | GRGCYC | 1 cut(s) | 685 |
| Fsp4HI | GCNGC | 6 cut(s) | 280, 443, 468, 628, 777, 951 |
| GlaI | GCGC | 4 cut(s) | 441, 631, 651, 780 |
| GluI | GCNGC | 6 cut(s) | 280, 443, 468, 628, 777, 951 |
| GsaI | CCCAGC | 2 cut(s) | 795, 885 |
| GsuI | CTGGAG | 1 cut(s) | 376 |
| HaeII | RGCGCY | 3 cut(s) | 443, 653, 782 |
| HaeIII | GGCC | 4 cut(s) | 309, 320, 475, 740 |
| HapII | CCGG | 4 cut(s) | 267, 306, 369, 429 |
| HgaI | GACGC | 2 cut(s) | 220, 352 |
| HhaI | GCGC | 4 cut(s) | 442, 632, 652, 781 |
| Hin1I | GRCGYC | 2 cut(s) | 231, 440 |
| Hin1II | CATG | 4 cut(s) | 193, 328, 421, 979 |
| Hin6I | GCGC | 4 cut(s) | 440, 630, 650, 779 |
| HinP1I | GCGC | 4 cut(s) | 440, 630, 650, 779 |
| HinfI | GANTC | 2 cut(s) | 56, 270 |
| HpaII | CCGG | 4 cut(s) | 267, 306, 369, 429 |
| HphI | GGTGA | 6 cut(s) | 40, 71, 235, 526, 572, 667 |
| Hpy166II | GTNNAC | 2 cut(s) | 766, 940 |
| Hpy188I | TCNGA | 3 cut(s) | 425, 643, 869 |
| Hpy188III | TCNNGA | 5 cut(s) | 381, 393, 418, 814, 829 |
| Hpy8I | GTNNAC | 2 cut(s) | 766, 940 |
| HpyAV | CCTTC | 6 cut(s) | 511, 652, 667, 715, 826, 871 |
| HpyCH4III | ACNGT | 3 cut(s) | 121, 404, 910 |
| HpyCH4IV | ACGT | 1 cut(s) | 285 |
| HpyCH4V | TGCA | 3 cut(s) | 896, 932, 953 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 360, 369, 464, 588 |
| HpySE526I | ACGT | 1 cut(s) | 285 |
| Hsp92I | GRCGYC | 2 cut(s) | 231, 440 |
| Hsp92II | CATG | 4 cut(s) | 193, 328, 421, 979 |
| HspAI | GCGC | 4 cut(s) | 440, 630, 650, 779 |
| KasI | GGCGCC | 1 cut(s) | 439 |
| Ksp22I | TGATCA | 2 cut(s) | 450, 492 |
| Kzo9I | GATC | 7 cut(s) | 420, 450, 492, 701, 825, 864, 926 |
| LmnI | GCTCC | 6 cut(s) | 143, 302, 425, 588, 680, 806 |
| Lsp1109I | GCAGC | 3 cut(s) | 291, 614, 937 |
| LweI | GCATC | 1 cut(s) | 840 |
| MaeII | ACGT | 1 cut(s) | 285 |
| MaeIII | GTNAC | 1 cut(s) | 241 |
| MalI | GATC | 7 cut(s) | 422, 452, 494, 703, 827, 866, 928 |
| MboI | GATC | 7 cut(s) | 420, 450, 492, 701, 825, 864, 926 |
| MboII | GAAGA | 4 cut(s) | 34, 99, 283, 496 |
| MflI | RGATCY | 1 cut(s) | 926 |
| MhlI | GDGCHC | 3 cut(s) | 436, 460, 685 |
| MlsI | TGGCCA | 1 cut(s) | 320 |
| MluCI | AATT | 2 cut(s) | 384, 637 |
| MluNI | TGGCCA | 1 cut(s) | 320 |
| Mly113I | GGCGCC | 1 cut(s) | 440 |
| MmeI | TCCRAC | 2 cut(s) | 679, 784 |
| Mox20I | TGGCCA | 1 cut(s) | 320 |
| MscI | TGGCCA | 1 cut(s) | 320 |
| Msp20I | TGGCCA | 1 cut(s) | 320 |
| MspA1I | CMGCKG | 1 cut(s) | 958 |
| MspI | CCGG | 4 cut(s) | 267, 306, 369, 429 |
| MspR9I | CCNGG | 4 cut(s) | 199, 267, 317, 477 |
| Mva1269I | GAATGC | 1 cut(s) | 712 |
| MvaI | CCWGG | 3 cut(s) | 199, 317, 477 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 360, 369, 464, 588 |
| NarI | GGCGCC | 1 cut(s) | 440 |
| NciI | CCSGG | 1 cut(s) | 267 |
| NcoI | CCATGG | 1 cut(s) | 324 |
| NdeI | CATATG | 1 cut(s) | 497 |
| NdeII | GATC | 7 cut(s) | 420, 450, 492, 701, 825, 864, 926 |
| NlaIII | CATG | 4 cut(s) | 193, 328, 421, 979 |
| NlaIV | GGNNCC | 2 cut(s) | 427, 441 |
| NmeAIII | GCCGAG | 1 cut(s) | 207 |
| NmuCI | GTSAC | 1 cut(s) | 241 |
| PagI | TCATGA | 1 cut(s) | 417 |
| PceI | AGGCCT | 1 cut(s) | 475 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 282 |
| PctI | GAATGC | 1 cut(s) | 712 |
| PfeI | GAWTC | 2 cut(s) | 56, 270 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 316 |
| PkrI | GCNGC | 6 cut(s) | 281, 444, 469, 629, 778, 952 |
| PluTI | GGCGCC | 1 cut(s) | 443 |
| Psp124BI | GAGCTC | 1 cut(s) | 685 |
| Psp6I | CCWGG | 3 cut(s) | 197, 315, 475 |
| PspFI | CCCAGC | 2 cut(s) | 791, 881 |
| PspGI | CCWGG | 3 cut(s) | 197, 315, 475 |
| PspN4I | GGNNCC | 2 cut(s) | 427, 441 |
| PspPI | GGNCC | 1 cut(s) | 201 |
| PstI | CTGCAG | 1 cut(s) | 934 |
| PsuI | RGATCY | 1 cut(s) | 926 |
| PvuII | CAGCTG | 1 cut(s) | 958 |
| SacI | GAGCTC | 1 cut(s) | 685 |
| SatI | GCNGC | 6 cut(s) | 280, 443, 468, 628, 777, 951 |
| Sau3AI | GATC | 7 cut(s) | 420, 450, 492, 701, 825, 864, 926 |
| Sau96I | GGNCC | 1 cut(s) | 201 |
| ScrFI | CCNGG | 4 cut(s) | 199, 267, 317, 477 |
| SduI | GDGCHC | 3 cut(s) | 436, 460, 685 |
| SfaNI | GCATC | 1 cut(s) | 840 |
| SfcI | CTRYAG | 1 cut(s) | 930 |
| SfoI | GGCGCC | 1 cut(s) | 441 |
| SinI | GGWCC | 1 cut(s) | 201 |
| Sse9I | AATT | 2 cut(s) | 384, 637 |
| SseBI | AGGCCT | 1 cut(s) | 475 |
| SsiI | CCGC | 8 cut(s) | 148, 352, 357, 443, 467, 533, 602, 776 |
| SspDI | GGCGCC | 1 cut(s) | 439 |
| SstI | GAGCTC | 1 cut(s) | 685 |
| StuI | AGGCCT | 1 cut(s) | 475 |
| StyD4I | CCNGG | 4 cut(s) | 197, 265, 315, 475 |
| StyI | CCWWGG | 1 cut(s) | 324 |
| TaaI | ACNGT | 3 cut(s) | 121, 404, 910 |
| TaiI | ACGT | 1 cut(s) | 288 |
| TaqI | TCGA | 3 cut(s) | 93, 716, 813 |
| TasI | AATT | 2 cut(s) | 384, 637 |
| TauI | GCSGC | 3 cut(s) | 445, 470, 779 |
| TfiI | GAWTC | 2 cut(s) | 56, 270 |
| TscAI | CASTG | 2 cut(s) | 340, 409 |
| TseFI | GTSAC | 1 cut(s) | 241 |
| TseI | GCWGC | 3 cut(s) | 279, 627, 950 |
| Tsp45I | GTSAC | 1 cut(s) | 241 |
| TspDTI | ATGAA | 3 cut(s) | 34, 734, 981 |
| TspGWI | ACGGA | 1 cut(s) | 524 |
| TspRI | CASTG | 2 cut(s) | 340, 409 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 316 |
| VpaK11BI | GGWCC | 1 cut(s) | 201 |
| XapI | RAATTY | 2 cut(s) | 384, 637 |