Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 3 cut(s) | 385, 467, 911 |
| AccBSI | CCGCTC | 1 cut(s) | 192 |
| AciI | CCGC | 2 cut(s) | 190, 1452 |
| AclWI | GGATC | 2 cut(s) | 678, 906 |
| AcsI | RAATTY | 7 cut(s) | 18, 565, 778, 985, 1053, 1247, 1329 |
| AfaI | GTAC | 4 cut(s) | 81, 126, 632, 1408 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 155, 942 |
| AgsI | TTSAA | 4 cut(s) | 200, 301, 839, 1109 |
| AjnI | CCWGG | 2 cut(s) | 894, 1323 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 326, 358, 837, 869 |
| AleI | CACNNNNGTG | 1 cut(s) | 749 |
| AluBI | AGCT | 6 cut(s) | 145, 278, 589, 685, 737, 1357 |
| AluI | AGCT | 6 cut(s) | 145, 278, 589, 685, 737, 1357 |
| Alw26I | GTCTC | 6 cut(s) | 274, 469, 521, 1119, 1233, 1306 |
| AlwI | GGATC | 2 cut(s) | 678, 906 |
| AoxI | GGCC | 3 cut(s) | 221, 254, 885 |
| ApeKI | GCWGC | 2 cut(s) | 284, 932 |
| ApoI | RAATTY | 7 cut(s) | 18, 565, 778, 985, 1053, 1247, 1329 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1283 |
| AspLEI | GCGC | 3 cut(s) | 211, 1078, 1231 |
| AspS9I | GGNCC | 1 cut(s) | 221 |
| AsuC2I | CCSGG | 1 cut(s) | 938 |
| AsuHPI | GGTGA | 6 cut(s) | 82, 179, 271, 407, 728, 736 |
| AsuII | TTCGAA | 1 cut(s) | 1287 |
| AsuNHI | GCTAGC | 1 cut(s) | 733 |
| AxyI | CCTNAGG | 1 cut(s) | 1070 |
| BanI | GGYRCC | 3 cut(s) | 385, 467, 911 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 344, 376 |
| BbsI | GAAGAC | 2 cut(s) | 334, 1425 |
| BbvCI | CCTCAGC | 1 cut(s) | 279 |
| BbvI | GCAGC | 2 cut(s) | 271, 919 |
| BccI | CCATC | 3 cut(s) | 139, 249, 896 |
| BciT130I | CCWGG | 2 cut(s) | 896, 1325 |
| BciVI | GTATCC | 1 cut(s) | 1476 |
| BcnI | CCSGG | 1 cut(s) | 938 |
| BcoDI | GTCTC | 6 cut(s) | 274, 469, 521, 1119, 1233, 1306 |
| BfaI | CTAG | 4 cut(s) | 173, 206, 479, 734 |
| BfmI | CTRYAG | 1 cut(s) | 678 |
| BfoI | RGCGCY | 1 cut(s) | 1232 |
| BfuI | GTATCC | 1 cut(s) | 1476 |
| BglII | AGATCT | 1 cut(s) | 294 |
| BisI | GCNGC | 2 cut(s) | 285, 933 |
| BlsI | GCNGC | 2 cut(s) | 286, 934 |
| Bme1390I | CCNGG | 3 cut(s) | 896, 938, 1325 |
| BmgT120I | GGNCC | 1 cut(s) | 221 |
| BmiI | GGNNCC | 3 cut(s) | 387, 469, 913 |
| BmrFI | CCNGG | 3 cut(s) | 896, 938, 1325 |
| BmsI | GCATC | 1 cut(s) | 910 |
| BmtI | GCTAGC | 1 cut(s) | 737 |
| BpiI | GAAGAC | 2 cut(s) | 334, 1425 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 272, 304 |
| BpmI | CTGGAG | 2 cut(s) | 1129, 1464 |
| Bpu10I | CCTNAGC | 1 cut(s) | 279 |
| Bpu14I | TTCGAA | 1 cut(s) | 1287 |
| BpuMI | CCSGG | 1 cut(s) | 938 |
| BsaI | GGTCTC | 2 cut(s) | 1119, 1233 |
| BsaJI | CCNNGG | 6 cut(s) | 217, 313, 728, 936, 962, 1324 |
| BsaWI | WCCGGW | 1 cut(s) | 908 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1303, 1333 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 155, 942 |
| Bse118I | RCCGGY | 2 cut(s) | 168, 256 |
| Bse1I | ACTGG | 2 cut(s) | 1146, 1447 |
| Bse21I | CCTNAGG | 1 cut(s) | 1070 |
| Bse3DI | GCAATG | 3 cut(s) | 1120, 1152, 1347 |
| BseBI | CCWGG | 2 cut(s) | 896, 1325 |
| BseDI | CCNNGG | 6 cut(s) | 217, 313, 728, 936, 962, 1324 |
| BseGI | GGATG | 3 cut(s) | 891, 1139, 1495 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 155, 942 |
| BseMI | GCAATG | 3 cut(s) | 1120, 1152, 1347 |
| BseMII | CTCAG | 3 cut(s) | 259, 270, 1084 |
| BseNI | ACTGG | 2 cut(s) | 1146, 1447 |
| BseRI | GAGGAG | 2 cut(s) | 529, 532 |
| BseXI | GCAGC | 2 cut(s) | 271, 919 |
| BseYI | CCCAGC | 1 cut(s) | 1307 |
| BshFI | GGCC | 3 cut(s) | 223, 256, 887 |
| BshNI | GGYRCC | 3 cut(s) | 385, 467, 911 |
| BsiSI | CCGG | 4 cut(s) | 169, 257, 909, 938 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 155, 942 |
| BsmAI | GTCTC | 6 cut(s) | 274, 469, 521, 1119, 1233, 1306 |
| BsmBI | CGTCTC | 1 cut(s) | 1306 |
| BsmI | GAATGC | 1 cut(s) | 1102 |
| BsnI | GGCC | 3 cut(s) | 223, 256, 887 |
| Bso31I | GGTCTC | 2 cut(s) | 1119, 1233 |
| Bsp119I | TTCGAA | 1 cut(s) | 1287 |
| Bsp143I | GATC | 8 cut(s) | 153, 226, 294, 637, 670, 868, 898, 1191 |
| Bsp19I | CCATGG | 2 cut(s) | 217, 728 |
| BspACI | CCGC | 2 cut(s) | 190, 1452 |
| BspANI | GGCC | 3 cut(s) | 223, 256, 887 |
| BspCNI | CTCAG | 3 cut(s) | 258, 271, 1083 |
| BspHI | TCATGA | 1 cut(s) | 495 |
| BspLI | GGNNCC | 3 cut(s) | 387, 469, 913 |
| BspOI | GCTAGC | 1 cut(s) | 737 |
| BspPI | GGATC | 2 cut(s) | 678, 906 |
| BspT104I | TTCGAA | 1 cut(s) | 1287 |
| BspT107I | GGYRCC | 3 cut(s) | 385, 467, 911 |
| BspTNI | GGTCTC | 2 cut(s) | 1119, 1233 |
| BsrBI | CCGCTC | 1 cut(s) | 192 |
| BsrDI | GCAATG | 3 cut(s) | 1120, 1152, 1347 |
| BsrFI | RCCGGY | 2 cut(s) | 168, 256 |
| BsrI | ACTGG | 2 cut(s) | 1146, 1447 |
| BssAI | RCCGGY | 2 cut(s) | 168, 256 |
| BssECI | CCNNGG | 6 cut(s) | 217, 313, 728, 936, 962, 1324 |
| BssMI | GATC | 8 cut(s) | 153, 226, 294, 637, 670, 868, 898, 1191 |
| BssT1I | CCWWGG | 4 cut(s) | 217, 313, 728, 962 |
| Bst2UI | CCWGG | 2 cut(s) | 896, 1325 |
| Bst4CI | ACNGT | 3 cut(s) | 472, 516, 635 |
| Bst6I | CTCTTC | 2 cut(s) | 201, 1231 |
| BstBI | TTCGAA | 1 cut(s) | 1287 |
| BstC8I | GCNNGC | 3 cut(s) | 254, 735, 1359 |
| BstDEI | CTNAG | 4 cut(s) | 245, 279, 1070, 1423 |
| BstDSI | CCRYGG | 2 cut(s) | 217, 728 |
| BstF5I | GGATG | 3 cut(s) | 891, 1139, 1495 |
| BstH2I | RGCGCY | 1 cut(s) | 1232 |
| BstHHI | GCGC | 3 cut(s) | 211, 1078, 1231 |
| BstKTI | GATC | 8 cut(s) | 156, 229, 297, 640, 673, 871, 901, 1194 |
| BstMAI | GTCTC | 6 cut(s) | 274, 469, 521, 1119, 1233, 1306 |
| BstMBI | GATC | 8 cut(s) | 153, 226, 294, 637, 670, 868, 898, 1191 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 284, 920, 929, 1065 |
| BstNI | CCWGG | 2 cut(s) | 896, 1325 |
| BstSCI | CCNGG | 3 cut(s) | 894, 936, 1323 |
| BstSFI | CTRYAG | 1 cut(s) | 678 |
| BstV1I | GCAGC | 2 cut(s) | 271, 919 |
| BstV2I | GAAGAC | 2 cut(s) | 334, 1425 |
| BstX2I | RGATCY | 3 cut(s) | 294, 670, 898 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 895 |
| BstYI | RGATCY | 3 cut(s) | 294, 670, 898 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1070 |
| BsuI | GTATCC | 1 cut(s) | 1476 |
| BsuRI | GGCC | 3 cut(s) | 223, 256, 887 |
| BtgI | CCRYGG | 2 cut(s) | 217, 728 |
| BtsCI | GGATG | 3 cut(s) | 891, 1139, 1495 |
| BtsI | GCAGTG | 1 cut(s) | 1485 |
| BtsIMutI | CAGTG | 4 cut(s) | 640, 915, 1440, 1485 |
| Cac8I | GCNNGC | 3 cut(s) | 254, 735, 1359 |
| CciI | TCATGA | 1 cut(s) | 495 |
| CfoI | GCGC | 3 cut(s) | 211, 1078, 1231 |
| Cfr10I | RCCGGY | 2 cut(s) | 168, 256 |
| Cfr13I | GGNCC | 1 cut(s) | 221 |
| Csp6I | GTAC | 4 cut(s) | 80, 125, 631, 1407 |
| CviAII | CATG | 7 cut(s) | 218, 308, 496, 729, 1224, 1403, 1429 |
| CviQI | GTAC | 4 cut(s) | 80, 125, 631, 1407 |
| DdeI | CTNAG | 4 cut(s) | 245, 279, 1070, 1423 |
| DpnI | GATC | 8 cut(s) | 155, 228, 296, 639, 672, 870, 900, 1193 |
| DpnII | GATC | 8 cut(s) | 153, 226, 294, 637, 670, 868, 898, 1191 |
| DraI | TTTAAA | 3 cut(s) | 322, 564, 783 |
| Eam1104I | CTCTTC | 2 cut(s) | 201, 1231 |
| EarI | CTCTTC | 2 cut(s) | 201, 1231 |
| Eco130I | CCWWGG | 4 cut(s) | 217, 313, 728, 962 |
| Eco31I | GGTCTC | 2 cut(s) | 1119, 1233 |
| Eco81I | CCTNAGG | 1 cut(s) | 1070 |
| EcoRI | GAATTC | 1 cut(s) | 1329 |
| EcoRII | CCWGG | 2 cut(s) | 894, 1323 |
| EcoT14I | CCWWGG | 4 cut(s) | 217, 313, 728, 962 |
| EcoT22I | ATGCAT | 1 cut(s) | 1225 |
| ErhI | CCWWGG | 4 cut(s) | 217, 313, 728, 962 |
| Esp3I | CGTCTC | 1 cut(s) | 1306 |
| FaeI | CATG | 7 cut(s) | 221, 311, 499, 732, 1227, 1406, 1432 |
| FalI | AAGNNNNNCTT | 3 cut(s) | 326, 358, 1495 |
| FatI | CATG | 7 cut(s) | 217, 307, 495, 728, 1223, 1402, 1428 |
| FauNDI | CATATG | 1 cut(s) | 762 |
| Fnu4HI | GCNGC | 2 cut(s) | 285, 933 |
| FokI | GGATG | 3 cut(s) | 878, 1126, 1482 |
| Fsp4HI | GCNGC | 2 cut(s) | 285, 933 |
| FspBI | CTAG | 4 cut(s) | 173, 206, 479, 734 |
| GlaI | GCGC | 3 cut(s) | 210, 1077, 1230 |
| GluI | GCNGC | 2 cut(s) | 285, 933 |
| GsaI | CCCAGC | 1 cut(s) | 1311 |
| GsuI | CTGGAG | 2 cut(s) | 1129, 1464 |
| HaeII | RGCGCY | 1 cut(s) | 1232 |
| HaeIII | GGCC | 3 cut(s) | 223, 256, 887 |
| HapII | CCGG | 4 cut(s) | 169, 257, 909, 938 |
| HhaI | GCGC | 3 cut(s) | 211, 1078, 1231 |
| Hin1II | CATG | 7 cut(s) | 221, 311, 499, 732, 1227, 1406, 1432 |
| Hin6I | GCGC | 3 cut(s) | 209, 1076, 1229 |
| HinP1I | GCGC | 3 cut(s) | 209, 1076, 1229 |
| HindIII | AAGCTT | 2 cut(s) | 143, 587 |
| HinfI | GANTC | 2 cut(s) | 967, 1508 |
| HpaII | CCGG | 4 cut(s) | 169, 257, 909, 938 |
| HphI | GGTGA | 6 cut(s) | 82, 179, 271, 407, 728, 736 |
| Hpy166II | GTNNAC | 2 cut(s) | 744, 945 |
| Hpy188I | TCNGA | 7 cut(s) | 158, 248, 292, 351, 644, 955, 1016 |
| Hpy188III | TCNNGA | 2 cut(s) | 23, 496 |
| Hpy8I | GTNNAC | 2 cut(s) | 744, 945 |
| Hpy99I | CGWCG | 1 cut(s) | 559 |
| HpyAV | CCTTC | 3 cut(s) | 346, 641, 1103 |
| HpyCH4III | ACNGT | 3 cut(s) | 472, 516, 635 |
| HpyCH4IV | ACGT | 1 cut(s) | 1029 |
| HpyCH4V | TGCA | 7 cut(s) | 16, 851, 991, 1100, 1223, 1340, 1378 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 284, 920, 929, 1065 |
| HpyF3I | CTNAG | 4 cut(s) | 245, 279, 1070, 1423 |
| HpySE526I | ACGT | 1 cut(s) | 1029 |
| Hsp92II | CATG | 7 cut(s) | 221, 311, 499, 732, 1227, 1406, 1432 |
| HspAI | GCGC | 3 cut(s) | 209, 1076, 1229 |
| Kzo9I | GATC | 8 cut(s) | 153, 226, 294, 637, 670, 868, 898, 1191 |
| LmnI | GCTCC | 1 cut(s) | 1445 |
| Lsp1109I | GCAGC | 2 cut(s) | 271, 919 |
| LweI | GCATC | 1 cut(s) | 910 |
| MaeI | CTAG | 4 cut(s) | 173, 206, 479, 734 |
| MaeII | ACGT | 1 cut(s) | 1029 |
| MaeIII | GTNAC | 4 cut(s) | 177, 716, 1030, 1184 |
| MalI | GATC | 8 cut(s) | 155, 228, 296, 639, 672, 870, 900, 1193 |
| MbiI | CCGCTC | 1 cut(s) | 192 |
| MboI | GATC | 8 cut(s) | 153, 226, 294, 637, 670, 868, 898, 1191 |
| MflI | RGATCY | 3 cut(s) | 294, 670, 898 |
| MmeI | TCCRAC | 1 cut(s) | 994 |
| Mph1103I | ATGCAT | 1 cut(s) | 1225 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1283 |
| MseI | TTAA | 8 cut(s) | 321, 431, 563, 569, 782, 1085, 1218, 1350 |
| MslI | CAYNNNNRTG | 2 cut(s) | 749, 1095 |
| MspA1I | CMGCKG | 1 cut(s) | 278 |
| MspI | CCGG | 4 cut(s) | 169, 257, 909, 938 |
| MspR9I | CCNGG | 3 cut(s) | 896, 938, 1325 |
| Mva1269I | GAATGC | 1 cut(s) | 1102 |
| MvaI | CCWGG | 2 cut(s) | 896, 1325 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 284, 920, 929, 1065 |
| NciI | CCSGG | 1 cut(s) | 938 |
| NcoI | CCATGG | 2 cut(s) | 217, 728 |
| NdeI | CATATG | 1 cut(s) | 762 |
| NdeII | GATC | 8 cut(s) | 153, 226, 294, 637, 670, 868, 898, 1191 |
| NheI | GCTAGC | 1 cut(s) | 733 |
| NlaIII | CATG | 7 cut(s) | 221, 311, 499, 732, 1227, 1406, 1432 |
| NlaIV | GGNNCC | 3 cut(s) | 387, 469, 913 |
| NmuCI | GTSAC | 1 cut(s) | 716 |
| NsiI | ATGCAT | 1 cut(s) | 1225 |
| NspV | TTCGAA | 1 cut(s) | 1287 |
| OliI | CACNNNNGTG | 1 cut(s) | 749 |
| PagI | TCATGA | 1 cut(s) | 495 |
| PctI | GAATGC | 1 cut(s) | 1102 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1283 |
| PfeI | GAWTC | 2 cut(s) | 967, 1508 |
| PflFI | GACNNNGTC | 1 cut(s) | 949 |
| PfoI | TCCNGGA | 1 cut(s) | 894 |
| PkrI | GCNGC | 2 cut(s) | 286, 934 |
| Psp6I | CCWGG | 2 cut(s) | 894, 1323 |
| PspFI | CCCAGC | 1 cut(s) | 1307 |
| PspGI | CCWGG | 2 cut(s) | 894, 1323 |
| PspN4I | GGNNCC | 3 cut(s) | 387, 469, 913 |
| PspPI | GGNCC | 1 cut(s) | 221 |
| PsuI | RGATCY | 3 cut(s) | 294, 670, 898 |
| PsyI | GACNNNGTC | 1 cut(s) | 949 |
| PvuII | CAGCTG | 1 cut(s) | 278 |
| RsaI | GTAC | 4 cut(s) | 81, 126, 632, 1408 |
| RsaNI | GTAC | 4 cut(s) | 80, 125, 631, 1407 |
| RseI | CAYNNNNRTG | 2 cut(s) | 749, 1095 |
| SaqAI | TTAA | 8 cut(s) | 321, 431, 563, 569, 782, 1085, 1218, 1350 |
| SatI | GCNGC | 2 cut(s) | 285, 933 |
| Sau3AI | GATC | 8 cut(s) | 153, 226, 294, 637, 670, 868, 898, 1191 |
| Sau96I | GGNCC | 1 cut(s) | 221 |
| ScrFI | CCNGG | 3 cut(s) | 896, 938, 1325 |
| SfaNI | GCATC | 1 cut(s) | 910 |
| SfcI | CTRYAG | 1 cut(s) | 678 |
| SfuI | TTCGAA | 1 cut(s) | 1287 |
| SmiI | ATTTAAAT | 1 cut(s) | 783 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 749, 1095 |
| SsiI | CCGC | 2 cut(s) | 190, 1452 |
| SspI | AATATT | 1 cut(s) | 712 |
| SspMI | CTAG | 4 cut(s) | 173, 206, 479, 734 |
| StyD4I | CCNGG | 3 cut(s) | 894, 936, 1323 |
| StyI | CCWWGG | 4 cut(s) | 217, 313, 728, 962 |
| SwaI | ATTTAAAT | 1 cut(s) | 783 |
| TaaI | ACNGT | 3 cut(s) | 472, 516, 635 |
| TaiI | ACGT | 1 cut(s) | 1032 |
| TaqI | TCGA | 3 cut(s) | 1133, 1287, 1386 |
| TatI | WGTACW | 1 cut(s) | 630 |
| TfiI | GAWTC | 2 cut(s) | 967, 1508 |
| Tru1I | TTAA | 8 cut(s) | 321, 431, 563, 569, 782, 1085, 1218, 1350 |
| Tru9I | TTAA | 8 cut(s) | 321, 431, 563, 569, 782, 1085, 1218, 1350 |
| TscAI | CASTG | 4 cut(s) | 640, 922, 1447, 1485 |
| TseFI | GTSAC | 1 cut(s) | 716 |
| TseI | GCWGC | 2 cut(s) | 284, 932 |
| Tsp45I | GTSAC | 1 cut(s) | 716 |
| TspDTI | ATGAA | 7 cut(s) | 599, 995, 1011, 1035, 1079, 1287, 1484 |
| TspRI | CASTG | 4 cut(s) | 640, 922, 1447, 1485 |
| Tth111I | GACNNNGTC | 1 cut(s) | 949 |
| XapI | RAATTY | 7 cut(s) | 18, 565, 778, 985, 1053, 1247, 1329 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 735 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1283 |
| XspI | CTAG | 4 cut(s) | 173, 206, 479, 734 |
| Zsp2I | ATGCAT | 1 cut(s) | 1225 |