Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 388 |
| Acc36I | ACCTGC | 1 cut(s) | 388 |
| AccII | CGCG | 1 cut(s) | 456 |
| AciI | CCGC | 3 cut(s) | 5, 330, 456 |
| AclWI | GGATC | 1 cut(s) | 474 |
| AcoI | YGGCCR | 1 cut(s) | 264 |
| AfaI | GTAC | 4 cut(s) | 39, 68, 97, 207 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 137 |
| AgsI | TTSAA | 1 cut(s) | 191 |
| AjiI | CACGTC | 1 cut(s) | 364 |
| AluBI | AGCT | 2 cut(s) | 10, 343 |
| AluI | AGCT | 2 cut(s) | 10, 343 |
| Alw21I | GWGCWC | 2 cut(s) | 12, 187 |
| Alw26I | GTCTC | 2 cut(s) | 262, 467 |
| Alw44I | GTGCAC | 1 cut(s) | 183 |
| AlwI | GGATC | 1 cut(s) | 474 |
| Ama87I | CYCGRG | 3 cut(s) | 162, 201, 276 |
| AoxI | GGCC | 1 cut(s) | 264 |
| ApaLI | GTGCAC | 1 cut(s) | 183 |
| ApeKI | GCWGC | 1 cut(s) | 272 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 290, 322 |
| AspLEI | GCGC | 3 cut(s) | 161, 376, 434 |
| AspS9I | GGNCC | 2 cut(s) | 212, 447 |
| AsuC2I | CCSGG | 2 cut(s) | 277, 278 |
| AsuHPI | GGTGA | 3 cut(s) | 140, 395, 412 |
| AvaI | CYCGRG | 3 cut(s) | 162, 201, 276 |
| AvaII | GGWCC | 2 cut(s) | 212, 447 |
| BaeGI | GKGCMC | 1 cut(s) | 187 |
| BanII | GRGCYC | 1 cut(s) | 12 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 332, 364 |
| BbsI | GAAGAC | 1 cut(s) | 69 |
| Bbv12I | GWGCWC | 2 cut(s) | 12, 187 |
| BbvI | GCAGC | 1 cut(s) | 259 |
| BccI | CCATC | 2 cut(s) | 140, 186 |
| BceAI | ACGGC | 1 cut(s) | 32 |
| BclI | TGATCA | 1 cut(s) | 486 |
| BcnI | CCSGG | 2 cut(s) | 277, 278 |
| BcoDI | GTCTC | 2 cut(s) | 262, 467 |
| BfaI | CTAG | 1 cut(s) | 155 |
| BfoI | RGCGCY | 2 cut(s) | 162, 435 |
| BfuAI | ACCTGC | 1 cut(s) | 388 |
| BisI | GCNGC | 2 cut(s) | 273, 457 |
| BlsI | GCNGC | 2 cut(s) | 274, 458 |
| Bme1390I | CCNGG | 2 cut(s) | 277, 278 |
| Bme18I | GGWCC | 2 cut(s) | 212, 447 |
| BmeT110I | CYCGRG | 3 cut(s) | 162, 201, 276 |
| BmgBI | CACGTC | 1 cut(s) | 364 |
| BmgT120I | GGNCC | 2 cut(s) | 212, 447 |
| BmrFI | CCNGG | 2 cut(s) | 277, 278 |
| BoxI | GACNNNNGTC | 1 cut(s) | 322 |
| BpiI | GAAGAC | 1 cut(s) | 69 |
| BpmI | CTGGAG | 1 cut(s) | 412 |
| BpuEI | CTTGAG | 1 cut(s) | 448 |
| BpuMI | CCSGG | 2 cut(s) | 277, 278 |
| BsaI | GGTCTC | 1 cut(s) | 467 |
| BsaJI | CCNNGG | 2 cut(s) | 267, 276 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 111, 141 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 137 |
| Bse1I | ACTGG | 2 cut(s) | 90, 395 |
| BseDI | CCNNGG | 2 cut(s) | 267, 276 |
| BseGI | GGATG | 1 cut(s) | 57 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 137 |
| BseNI | ACTGG | 2 cut(s) | 90, 395 |
| BseRI | GAGGAG | 2 cut(s) | 215, 485 |
| BseSI | GKGCMC | 1 cut(s) | 187 |
| BseX3I | CGGCCG | 1 cut(s) | 264 |
| BseXI | GCAGC | 1 cut(s) | 259 |
| Bsh1236I | CGCG | 1 cut(s) | 456 |
| Bsh1285I | CGRYCG | 1 cut(s) | 267 |
| BshFI | GGCC | 1 cut(s) | 266 |
| BsiEI | CGRYCG | 1 cut(s) | 267 |
| BsiHKAI | GWGCWC | 2 cut(s) | 12, 187 |
| BsiHKCI | CYCGRG | 3 cut(s) | 162, 201, 276 |
| BsiSI | CCGG | 1 cut(s) | 277 |
| BslFI | GGGAC | 1 cut(s) | 227 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 137 |
| BsmAI | GTCTC | 2 cut(s) | 262, 467 |
| BsmBI | CGTCTC | 1 cut(s) | 262 |
| BsmFI | GGGAC | 1 cut(s) | 227 |
| BsmI | GAATGC | 1 cut(s) | 305 |
| BsnI | GGCC | 1 cut(s) | 266 |
| Bso31I | GGTCTC | 1 cut(s) | 467 |
| BsoBI | CYCGRG | 3 cut(s) | 162, 201, 276 |
| Bsp1286I | GDGCHC | 2 cut(s) | 12, 187 |
| Bsp1407I | TGTACA | 1 cut(s) | 66 |
| Bsp143I | GATC | 3 cut(s) | 282, 479, 486 |
| BspACI | CCGC | 3 cut(s) | 5, 330, 456 |
| BspANI | GGCC | 1 cut(s) | 266 |
| BspFNI | CGCG | 1 cut(s) | 456 |
| BspHI | TCATGA | 1 cut(s) | 285 |
| BspMI | ACCTGC | 1 cut(s) | 388 |
| BspPI | GGATC | 1 cut(s) | 474 |
| BspQI | GCTCTTC | 1 cut(s) | 398 |
| BspTNI | GGTCTC | 1 cut(s) | 467 |
| BsrGI | TGTACA | 1 cut(s) | 66 |
| BsrI | ACTGG | 2 cut(s) | 90, 395 |
| BssECI | CCNNGG | 2 cut(s) | 267, 276 |
| BssMI | GATC | 3 cut(s) | 282, 479, 486 |
| Bst4CI | ACNGT | 1 cut(s) | 383 |
| Bst6I | CTCTTC | 3 cut(s) | 232, 398, 462 |
| BstAUI | TGTACA | 1 cut(s) | 66 |
| BstC8I | GCNNGC | 2 cut(s) | 313, 416 |
| BstDEI | CTNAG | 1 cut(s) | 321 |
| BstF5I | GGATG | 1 cut(s) | 57 |
| BstFNI | CGCG | 1 cut(s) | 456 |
| BstH2I | RGCGCY | 2 cut(s) | 162, 435 |
| BstHHI | GCGC | 3 cut(s) | 161, 376, 434 |
| BstKTI | GATC | 3 cut(s) | 285, 482, 489 |
| BstMAI | GTCTC | 2 cut(s) | 262, 467 |
| BstMBI | GATC | 3 cut(s) | 282, 479, 486 |
| BstMCI | CGRYCG | 1 cut(s) | 267 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 191, 272 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 322 |
| BstSCI | CCNGG | 2 cut(s) | 275, 276 |
| BstSLI | GKGCMC | 1 cut(s) | 187 |
| BstUI | CGCG | 1 cut(s) | 456 |
| BstV1I | GCAGC | 1 cut(s) | 259 |
| BstV2I | GAAGAC | 1 cut(s) | 69 |
| BstX2I | RGATCY | 1 cut(s) | 479 |
| BstYI | RGATCY | 1 cut(s) | 479 |
| BstZI | CGGCCG | 1 cut(s) | 264 |
| BsuRI | GGCC | 1 cut(s) | 266 |
| BtrI | CACGTC | 1 cut(s) | 364 |
| BtsCI | GGATG | 1 cut(s) | 57 |
| BveI | ACCTGC | 1 cut(s) | 388 |
| Cac8I | GCNNGC | 2 cut(s) | 313, 416 |
| CciI | TCATGA | 1 cut(s) | 285 |
| CfoI | GCGC | 3 cut(s) | 161, 376, 434 |
| Cfr13I | GGNCC | 2 cut(s) | 212, 447 |
| Cfr9I | CCCGGG | 1 cut(s) | 276 |
| Csp6I | GTAC | 4 cut(s) | 38, 67, 96, 206 |
| CviAII | CATG | 5 cut(s) | 209, 220, 286, 296, 484 |
| CviJI | RGCY | 5 cut(s) | 10, 194, 266, 272, 343 |
| CviKI_1 | RGCY | 5 cut(s) | 10, 194, 266, 272, 343 |
| CviQI | GTAC | 4 cut(s) | 38, 67, 96, 206 |
| DdeI | CTNAG | 1 cut(s) | 321 |
| DpnI | GATC | 3 cut(s) | 284, 481, 488 |
| DpnII | GATC | 3 cut(s) | 282, 479, 486 |
| EaeI | YGGCCR | 1 cut(s) | 264 |
| EagI | CGGCCG | 1 cut(s) | 264 |
| Eam1104I | CTCTTC | 3 cut(s) | 232, 398, 462 |
| EarI | CTCTTC | 3 cut(s) | 232, 398, 462 |
| EciI | GGCGGA | 1 cut(s) | 20 |
| Ecl136II | GAGCTC | 1 cut(s) | 10 |
| EclXI | CGGCCG | 1 cut(s) | 264 |
| Eco24I | GRGCYC | 1 cut(s) | 12 |
| Eco31I | GGTCTC | 1 cut(s) | 467 |
| Eco47I | GGWCC | 2 cut(s) | 212, 447 |
| Eco52I | CGGCCG | 1 cut(s) | 264 |
| Eco53kI | GAGCTC | 1 cut(s) | 10 |
| Eco88I | CYCGRG | 3 cut(s) | 162, 201, 276 |
| EcoICRI | GAGCTC | 1 cut(s) | 10 |
| EcoT38I | GRGCYC | 1 cut(s) | 12 |
| Esp3I | CGTCTC | 1 cut(s) | 262 |
| FaeI | CATG | 5 cut(s) | 212, 223, 289, 299, 487 |
| FaiI | YATR | 8 cut(s) | 210, 221, 287, 297, 379, 410, 412, 485 |
| FaqI | GGGAC | 1 cut(s) | 227 |
| FatI | CATG | 5 cut(s) | 208, 219, 285, 295, 483 |
| FauNDI | CATATG | 1 cut(s) | 410 |
| FbaI | TGATCA | 1 cut(s) | 486 |
| Fnu4HI | GCNGC | 2 cut(s) | 273, 457 |
| FokI | GGATG | 1 cut(s) | 44 |
| FriOI | GRGCYC | 1 cut(s) | 12 |
| Fsp4HI | GCNGC | 2 cut(s) | 273, 457 |
| FspBI | CTAG | 1 cut(s) | 155 |
| GlaI | GCGC | 3 cut(s) | 160, 375, 433 |
| GluI | GCNGC | 2 cut(s) | 273, 457 |
| GsuI | CTGGAG | 1 cut(s) | 412 |
| HaeII | RGCGCY | 2 cut(s) | 162, 435 |
| HaeIII | GGCC | 1 cut(s) | 266 |
| HapII | CCGG | 1 cut(s) | 277 |
| HhaI | GCGC | 3 cut(s) | 161, 376, 434 |
| Hin1II | CATG | 5 cut(s) | 212, 223, 289, 299, 487 |
| Hin6I | GCGC | 3 cut(s) | 159, 374, 432 |
| HinP1I | GCGC | 3 cut(s) | 159, 374, 432 |
| HindIII | AAGCTT | 1 cut(s) | 341 |
| HinfI | GANTC | 2 cut(s) | 240, 421 |
| HpaII | CCGG | 1 cut(s) | 277 |
| HphI | GGTGA | 3 cut(s) | 140, 395, 412 |
| Hpy166II | GTNNAC | 2 cut(s) | 185, 327 |
| Hpy188I | TCNGA | 2 cut(s) | 140, 236 |
| Hpy188III | TCNNGA | 4 cut(s) | 201, 286, 337, 427 |
| Hpy8I | GTNNAC | 2 cut(s) | 185, 327 |
| Hpy99I | CGWCG | 3 cut(s) | 47, 257, 265 |
| HpyAV | CCTTC | 4 cut(s) | 123, 155, 343, 433 |
| HpyCH4III | ACNGT | 1 cut(s) | 383 |
| HpyCH4IV | ACGT | 1 cut(s) | 363 |
| HpyCH4V | TGCA | 1 cut(s) | 185 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 191, 272 |
| HpyF3I | CTNAG | 1 cut(s) | 321 |
| HpySE526I | ACGT | 1 cut(s) | 363 |
| Hsp92II | CATG | 5 cut(s) | 212, 223, 289, 299, 487 |
| HspAI | GCGC | 3 cut(s) | 159, 374, 432 |
| Ksp22I | TGATCA | 1 cut(s) | 486 |
| Kzo9I | GATC | 3 cut(s) | 282, 479, 486 |
| LguI | GCTCTTC | 1 cut(s) | 398 |
| LmnI | GCTCC | 2 cut(s) | 7, 228 |
| LpnPI | CCDG | 6 cut(s) | 103, 290, 325, 376, 383, 490 |
| Lsp1109I | GCAGC | 1 cut(s) | 259 |
| MaeI | CTAG | 1 cut(s) | 155 |
| MaeII | ACGT | 1 cut(s) | 363 |
| MalI | GATC | 3 cut(s) | 284, 481, 488 |
| MboI | GATC | 3 cut(s) | 282, 479, 486 |
| MboII | GAAGA | 5 cut(s) | 69, 72, 249, 415, 479 |
| MflI | RGATCY | 1 cut(s) | 479 |
| MhlI | GDGCHC | 2 cut(s) | 12, 187 |
| MlyI | GAGTC | 1 cut(s) | 249 |
| MslI | CAYNNNNRTG | 1 cut(s) | 413 |
| MspI | CCGG | 1 cut(s) | 277 |
| MspR9I | CCNGG | 2 cut(s) | 277, 278 |
| Mva1269I | GAATGC | 1 cut(s) | 305 |
| MvnI | CGCG | 1 cut(s) | 456 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 191, 272 |
| NciI | CCSGG | 2 cut(s) | 277, 278 |
| NdeI | CATATG | 1 cut(s) | 410 |
| NdeII | GATC | 3 cut(s) | 282, 479, 486 |
| NlaIII | CATG | 5 cut(s) | 212, 223, 289, 299, 487 |
| NmeAIII | GCCGAG | 1 cut(s) | 292 |
| PaeR7I | CTCGAG | 2 cut(s) | 162, 201 |
| PagI | TCATGA | 1 cut(s) | 285 |
| PaqCI | CACCTGC | 1 cut(s) | 388 |
| PciSI | GCTCTTC | 1 cut(s) | 398 |
| PctI | GAATGC | 1 cut(s) | 305 |
| PfeI | GAWTC | 1 cut(s) | 421 |
| PkrI | GCNGC | 2 cut(s) | 274, 458 |
| PleI | GAGTC | 1 cut(s) | 248 |
| PpsI | GAGTC | 1 cut(s) | 248 |
| PshAI | GACNNNNGTC | 1 cut(s) | 322 |
| Psp124BI | GAGCTC | 1 cut(s) | 12 |
| PspPI | GGNCC | 2 cut(s) | 212, 447 |
| PspXI | VCTCGAGB | 1 cut(s) | 162 |
| PsuI | RGATCY | 1 cut(s) | 479 |
| RsaI | GTAC | 4 cut(s) | 39, 68, 97, 207 |
| RsaNI | GTAC | 4 cut(s) | 38, 67, 96, 206 |
| RseI | CAYNNNNRTG | 1 cut(s) | 413 |
| SacI | GAGCTC | 1 cut(s) | 12 |
| SapI | GCTCTTC | 1 cut(s) | 398 |
| SatI | GCNGC | 2 cut(s) | 273, 457 |
| Sau3AI | GATC | 3 cut(s) | 282, 479, 486 |
| Sau96I | GGNCC | 2 cut(s) | 212, 447 |
| SchI | GAGTC | 1 cut(s) | 249 |
| ScrFI | CCNGG | 2 cut(s) | 277, 278 |
| SduI | GDGCHC | 2 cut(s) | 12, 187 |
| SetI | ASST | 9 cut(s) | 12, 115, 147, 303, 322, 345, 366, 402, 444 |
| Sfr274I | CTCGAG | 2 cut(s) | 162, 201 |
| SinI | GGWCC | 2 cut(s) | 212, 447 |
| SlaI | CTCGAG | 2 cut(s) | 162, 201 |
| SmaI | CCCGGG | 1 cut(s) | 278 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 413 |
| SmlI | CTYRAG | 3 cut(s) | 162, 201, 427 |
| SmoI | CTYRAG | 3 cut(s) | 162, 201, 427 |
| SsiI | CCGC | 3 cut(s) | 5, 330, 456 |
| SspMI | CTAG | 1 cut(s) | 155 |
| SstI | GAGCTC | 1 cut(s) | 12 |
| StyD4I | CCNGG | 2 cut(s) | 275, 276 |
| TaaI | ACNGT | 1 cut(s) | 383 |
| TaiI | ACGT | 1 cut(s) | 366 |
| TaqI | TCGA | 3 cut(s) | 163, 202, 336 |
| TaqII | GACCGA | 1 cut(s) | 435 |
| TatI | WGTACW | 3 cut(s) | 37, 66, 205 |
| TauI | GCSGC | 1 cut(s) | 459 |
| TfiI | GAWTC | 1 cut(s) | 421 |
| TseI | GCWGC | 1 cut(s) | 272 |
| TspDTI | ATGAA | 2 cut(s) | 36, 302 |
| TspMI | CCCGGG | 1 cut(s) | 276 |
| VneI | GTGCAC | 1 cut(s) | 183 |
| VpaK11BI | GGWCC | 2 cut(s) | 212, 447 |
| XhoI | CTCGAG | 2 cut(s) | 162, 201 |
| XmaI | CCCGGG | 1 cut(s) | 276 |
| XspI | CTAG | 1 cut(s) | 155 |