Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 352 |
| AccII | CGCG | 1 cut(s) | 258 |
| AciI | CCGC | 1 cut(s) | 256 |
| AclWI | GGATC | 2 cut(s) | 178, 916 |
| AcyI | GRCGYC | 1 cut(s) | 353 |
| AdeI | CACNNNGTG | 1 cut(s) | 802 |
| AfaI | GTAC | 3 cut(s) | 33, 85, 583 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 125, 604, 1128 |
| AflII | CTTAAG | 1 cut(s) | 511 |
| AgsI | TTSAA | 4 cut(s) | 400, 727, 1004, 1057 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 346, 877 |
| AhlI | ACTAGT | 1 cut(s) | 80 |
| AjnI | CCWGG | 3 cut(s) | 597, 782, 994 |
| AluBI | AGCT | 4 cut(s) | 179, 322, 619, 977 |
| AluI | AGCT | 4 cut(s) | 179, 322, 619, 977 |
| Alw26I | GTCTC | 6 cut(s) | 241, 333, 367, 672, 737, 864 |
| AlwI | GGATC | 2 cut(s) | 178, 916 |
| Ama87I | CYCGRG | 2 cut(s) | 5, 875 |
| AoxI | GGCC | 1 cut(s) | 190 |
| ApeKI | GCWGC | 1 cut(s) | 322 |
| AspLEI | GCGC | 1 cut(s) | 355 |
| AspS9I | GGNCC | 1 cut(s) | 191 |
| AsuHPI | GGTGA | 1 cut(s) | 128 |
| AvaI | CYCGRG | 2 cut(s) | 5, 875 |
| BaeGI | GKGCMC | 1 cut(s) | 783 |
| BanI | GGYRCC | 1 cut(s) | 352 |
| BanII | GRGCYC | 1 cut(s) | 256 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 21, 53, 311, 343 |
| BauI | CACGAG | 1 cut(s) | 837 |
| BbvCI | CCTCAGC | 1 cut(s) | 246 |
| BbvI | GCAGC | 1 cut(s) | 309 |
| BccI | CCATC | 3 cut(s) | 298, 357, 628 |
| BciT130I | CCWGG | 3 cut(s) | 599, 784, 996 |
| BclI | TGATCA | 1 cut(s) | 735 |
| BcoDI | GTCTC | 6 cut(s) | 241, 333, 367, 672, 737, 864 |
| BcuI | ACTAGT | 1 cut(s) | 80 |
| BfaI | CTAG | 5 cut(s) | 41, 81, 143, 776, 845 |
| BfmI | CTRYAG | 1 cut(s) | 650 |
| BfoI | RGCGCY | 1 cut(s) | 356 |
| BfrI | CTTAAG | 1 cut(s) | 511 |
| BisI | GCNGC | 1 cut(s) | 323 |
| BlsI | GCNGC | 1 cut(s) | 324 |
| BmcAI | AGTACT | 1 cut(s) | 33 |
| Bme1390I | CCNGG | 3 cut(s) | 599, 784, 996 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 346, 877 |
| BmeT110I | CYCGRG | 2 cut(s) | 5, 875 |
| BmgT120I | GGNCC | 1 cut(s) | 191 |
| BmiI | GGNNCC | 3 cut(s) | 193, 253, 354 |
| BmrFI | CCNGG | 3 cut(s) | 599, 784, 996 |
| BmsI | GCATC | 1 cut(s) | 637 |
| BpmI | CTGGAG | 1 cut(s) | 747 |
| Bpu10I | CCTNAGC | 2 cut(s) | 175, 246 |
| Bsa29I | ATCGAT | 1 cut(s) | 461 |
| BsaBI | GATNNNNATC | 1 cut(s) | 308 |
| BsaHI | GRCGYC | 1 cut(s) | 353 |
| BsaI | GGTCTC | 1 cut(s) | 864 |
| BsaJI | CCNNGG | 5 cut(s) | 124, 187, 240, 782, 994 |
| BsaWI | WCCGGW | 1 cut(s) | 710 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 99, 129 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 125, 604, 1128 |
| Bse1I | ACTGG | 3 cut(s) | 589, 764, 1117 |
| Bse3DI | GCAATG | 1 cut(s) | 615 |
| Bse8I | GATNNNNATC | 1 cut(s) | 308 |
| BseBI | CCWGG | 3 cut(s) | 599, 784, 996 |
| BseCI | ATCGAT | 1 cut(s) | 461 |
| BseDI | CCNNGG | 5 cut(s) | 124, 187, 240, 782, 994 |
| BseGI | GGATG | 3 cut(s) | 368, 628, 706 |
| BseJI | GATNNNNATC | 1 cut(s) | 308 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 125, 604, 1128 |
| BseMI | GCAATG | 1 cut(s) | 615 |
| BseMII | CTCAG | 3 cut(s) | 194, 237, 398 |
| BseNI | ACTGG | 3 cut(s) | 589, 764, 1117 |
| BseRI | GAGGAG | 3 cut(s) | 194, 229, 911 |
| BseSI | GKGCMC | 1 cut(s) | 783 |
| BseXI | GCAGC | 1 cut(s) | 309 |
| Bsh1236I | CGCG | 1 cut(s) | 258 |
| BshFI | GGCC | 1 cut(s) | 192 |
| BshNI | GGYRCC | 1 cut(s) | 352 |
| BshVI | ATCGAT | 1 cut(s) | 461 |
| BsiHKCI | CYCGRG | 2 cut(s) | 5, 875 |
| BsiSI | CCGG | 1 cut(s) | 711 |
| BslFI | GGGAC | 1 cut(s) | 206 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 125, 604, 1128 |
| BsmAI | GTCTC | 6 cut(s) | 241, 333, 367, 672, 737, 864 |
| BsmFI | GGGAC | 1 cut(s) | 206 |
| BsmI | GAATGC | 2 cut(s) | 720, 856 |
| BsnI | GGCC | 1 cut(s) | 192 |
| Bso31I | GGTCTC | 1 cut(s) | 864 |
| BsoBI | CYCGRG | 2 cut(s) | 5, 875 |
| Bsp1286I | GDGCHC | 2 cut(s) | 256, 783 |
| Bsp143I | GATC | 5 cut(s) | 183, 261, 465, 735, 921 |
| Bsp19I | CCATGG | 1 cut(s) | 187 |
| BspACI | CCGC | 1 cut(s) | 256 |
| BspANI | GGCC | 1 cut(s) | 192 |
| BspCNI | CTCAG | 3 cut(s) | 193, 238, 399 |
| BspDI | ATCGAT | 1 cut(s) | 461 |
| BspFNI | CGCG | 1 cut(s) | 258 |
| BspHI | TCATGA | 2 cut(s) | 264, 639 |
| BspLI | GGNNCC | 3 cut(s) | 193, 253, 354 |
| BspMAI | CTGCAG | 1 cut(s) | 654 |
| BspPI | GGATC | 2 cut(s) | 178, 916 |
| BspT107I | GGYRCC | 1 cut(s) | 352 |
| BspTI | CTTAAG | 1 cut(s) | 511 |
| BspTNI | GGTCTC | 1 cut(s) | 864 |
| BsrDI | GCAATG | 1 cut(s) | 615 |
| BsrI | ACTGG | 3 cut(s) | 589, 764, 1117 |
| BssECI | CCNNGG | 5 cut(s) | 124, 187, 240, 782, 994 |
| BssMI | GATC | 5 cut(s) | 183, 261, 465, 735, 921 |
| BssNI | GRCGYC | 1 cut(s) | 353 |
| BssSI | CACGAG | 1 cut(s) | 837 |
| BssT1I | CCWWGG | 1 cut(s) | 187 |
| Bst2BI | CACGAG | 1 cut(s) | 837 |
| Bst2UI | CCWGG | 3 cut(s) | 599, 784, 996 |
| Bst6I | CTCTTC | 4 cut(s) | 377, 441, 753, 862 |
| BstACI | GRCGYC | 1 cut(s) | 353 |
| BstAFI | CTTAAG | 1 cut(s) | 511 |
| BstC8I | GCNNGC | 2 cut(s) | 256, 755 |
| BstDEI | CTNAG | 4 cut(s) | 175, 180, 246, 407 |
| BstDSI | CCRYGG | 1 cut(s) | 187 |
| BstF5I | GGATG | 3 cut(s) | 368, 628, 706 |
| BstFNI | CGCG | 1 cut(s) | 258 |
| BstH2I | RGCGCY | 1 cut(s) | 356 |
| BstHHI | GCGC | 1 cut(s) | 355 |
| BstKTI | GATC | 5 cut(s) | 186, 264, 468, 738, 924 |
| BstMAI | GTCTC | 6 cut(s) | 241, 333, 367, 672, 737, 864 |
| BstMBI | GATC | 5 cut(s) | 183, 261, 465, 735, 921 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 159, 251, 432, 625 |
| BstNI | CCWGG | 3 cut(s) | 599, 784, 996 |
| BstSCI | CCNGG | 3 cut(s) | 597, 782, 994 |
| BstSFI | CTRYAG | 1 cut(s) | 650 |
| BstSLI | GKGCMC | 1 cut(s) | 783 |
| BstUI | CGCG | 1 cut(s) | 258 |
| BstV1I | GCAGC | 1 cut(s) | 309 |
| BstX2I | RGATCY | 1 cut(s) | 183 |
| BstYI | RGATCY | 1 cut(s) | 183 |
| Bsu15I | ATCGAT | 1 cut(s) | 461 |
| BsuRI | GGCC | 1 cut(s) | 192 |
| BsuTUI | ATCGAT | 1 cut(s) | 461 |
| BtgI | CCRYGG | 1 cut(s) | 187 |
| BtgZI | GCGATG | 1 cut(s) | 143 |
| BtsCI | GGATG | 3 cut(s) | 368, 628, 706 |
| BtsI | GCAGTG | 2 cut(s) | 738, 1011 |
| BtsIMutI | CAGTG | 2 cut(s) | 738, 1011 |
| Cac8I | GCNNGC | 2 cut(s) | 256, 755 |
| CciI | TCATGA | 2 cut(s) | 264, 639 |
| CfoI | GCGC | 1 cut(s) | 355 |
| Cfr13I | GGNCC | 1 cut(s) | 191 |
| ClaI | ATCGAT | 1 cut(s) | 461 |
| CseI | GACGC | 2 cut(s) | 208, 336 |
| Csp6I | GTAC | 3 cut(s) | 32, 84, 582 |
| CviAII | CATG | 8 cut(s) | 188, 199, 265, 275, 640, 691, 855, 1099 |
| CviQI | GTAC | 3 cut(s) | 32, 84, 582 |
| DdeI | CTNAG | 4 cut(s) | 175, 180, 246, 407 |
| DinI | GGCGCC | 1 cut(s) | 354 |
| DpnI | GATC | 5 cut(s) | 185, 263, 467, 737, 923 |
| DpnII | GATC | 5 cut(s) | 183, 261, 465, 735, 921 |
| DraI | TTTAAA | 1 cut(s) | 507 |
| DraIII | CACNNNGTG | 1 cut(s) | 802 |
| DriI | GACNNNNNGTC | 2 cut(s) | 346, 877 |
| Eam1104I | CTCTTC | 4 cut(s) | 377, 441, 753, 862 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 346, 877 |
| EarI | CTCTTC | 4 cut(s) | 377, 441, 753, 862 |
| Eco130I | CCWWGG | 1 cut(s) | 187 |
| Eco24I | GRGCYC | 1 cut(s) | 256 |
| Eco31I | GGTCTC | 1 cut(s) | 864 |
| Eco88I | CYCGRG | 2 cut(s) | 5, 875 |
| EcoRII | CCWGG | 3 cut(s) | 597, 782, 994 |
| EcoT14I | CCWWGG | 1 cut(s) | 187 |
| EcoT22I | ATGCAT | 1 cut(s) | 856 |
| EcoT38I | GRGCYC | 1 cut(s) | 256 |
| EgeI | GGCGCC | 1 cut(s) | 354 |
| EheI | GGCGCC | 1 cut(s) | 354 |
| ErhI | CCWWGG | 1 cut(s) | 187 |
| FaeI | CATG | 8 cut(s) | 191, 202, 268, 278, 643, 694, 858, 1102 |
| FaqI | GGGAC | 1 cut(s) | 206 |
| FatI | CATG | 8 cut(s) | 187, 198, 264, 274, 639, 690, 854, 1098 |
| FauI | CCCGC | 1 cut(s) | 263 |
| FbaI | TGATCA | 1 cut(s) | 735 |
| Fnu4HI | GCNGC | 1 cut(s) | 323 |
| FokI | GGATG | 3 cut(s) | 375, 615, 713 |
| FriOI | GRGCYC | 1 cut(s) | 256 |
| Fsp4HI | GCNGC | 1 cut(s) | 323 |
| FspBI | CTAG | 5 cut(s) | 41, 81, 143, 776, 845 |
| GlaI | GCGC | 1 cut(s) | 354 |
| GluI | GCNGC | 1 cut(s) | 323 |
| GsuI | CTGGAG | 1 cut(s) | 747 |
| HaeII | RGCGCY | 1 cut(s) | 356 |
| HaeIII | GGCC | 1 cut(s) | 192 |
| HapII | CCGG | 1 cut(s) | 711 |
| HgaI | GACGC | 2 cut(s) | 208, 336 |
| HhaI | GCGC | 1 cut(s) | 355 |
| Hin1I | GRCGYC | 1 cut(s) | 353 |
| Hin1II | CATG | 8 cut(s) | 191, 202, 268, 278, 643, 694, 858, 1102 |
| Hin6I | GCGC | 1 cut(s) | 353 |
| HinP1I | GCGC | 1 cut(s) | 353 |
| HinfI | GANTC | 7 cut(s) | 92, 400, 482, 661, 745, 878, 1106 |
| HpaII | CCGG | 1 cut(s) | 711 |
| HphI | GGTGA | 1 cut(s) | 128 |
| Hpy188I | TCNGA | 6 cut(s) | 128, 183, 338, 408, 633, 677 |
| Hpy188III | TCNNGA | 4 cut(s) | 265, 640, 893, 919 |
| Hpy99I | CGWCG | 1 cut(s) | 352 |
| HpyAV | CCTTC | 6 cut(s) | 111, 143, 205, 235, 721, 978 |
| HpyCH4V | TGCA | 5 cut(s) | 652, 718, 757, 854, 1048 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 159, 251, 432, 625 |
| HpyF3I | CTNAG | 4 cut(s) | 175, 180, 246, 407 |
| Hsp92I | GRCGYC | 1 cut(s) | 353 |
| Hsp92II | CATG | 8 cut(s) | 191, 202, 268, 278, 643, 694, 858, 1102 |
| HspAI | GCGC | 1 cut(s) | 353 |
| KasI | GGCGCC | 1 cut(s) | 352 |
| Ksp22I | TGATCA | 1 cut(s) | 735 |
| Kzo9I | GATC | 5 cut(s) | 183, 261, 465, 735, 921 |
| LmnI | GCTCC | 4 cut(s) | 207, 251, 974, 1076 |
| Lsp1109I | GCAGC | 1 cut(s) | 309 |
| LweI | GCATC | 1 cut(s) | 637 |
| MaeI | CTAG | 5 cut(s) | 41, 81, 143, 776, 845 |
| MaeIII | GTNAC | 2 cut(s) | 147, 815 |
| MalI | GATC | 5 cut(s) | 185, 263, 467, 737, 923 |
| MboI | GATC | 5 cut(s) | 183, 261, 465, 735, 921 |
| MboII | GAAGA | 7 cut(s) | 49, 394, 395, 458, 740, 879, 1100 |
| MfeI | CAATTG | 1 cut(s) | 1057 |
| MflI | RGATCY | 1 cut(s) | 183 |
| MhlI | GDGCHC | 2 cut(s) | 256, 783 |
| MluCI | AATT | 4 cut(s) | 331, 1004, 1057, 1064 |
| Mly113I | GGCGCC | 1 cut(s) | 353 |
| MlyI | GAGTC | 3 cut(s) | 86, 739, 887 |
| MmeI | TCCRAC | 2 cut(s) | 315, 982 |
| Mph1103I | ATGCAT | 1 cut(s) | 856 |
| MseI | TTAA | 6 cut(s) | 506, 512, 623, 825, 1020, 1143 |
| MspCI | CTTAAG | 1 cut(s) | 511 |
| MspI | CCGG | 1 cut(s) | 711 |
| MspR9I | CCNGG | 3 cut(s) | 599, 784, 996 |
| MunI | CAATTG | 1 cut(s) | 1057 |
| Mva1269I | GAATGC | 2 cut(s) | 720, 856 |
| MvaI | CCWGG | 3 cut(s) | 599, 784, 996 |
| MvnI | CGCG | 1 cut(s) | 258 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 159, 251, 432, 625 |
| NarI | GGCGCC | 1 cut(s) | 353 |
| NcoI | CCATGG | 1 cut(s) | 187 |
| NdeII | GATC | 5 cut(s) | 183, 261, 465, 735, 921 |
| NlaIII | CATG | 8 cut(s) | 191, 202, 268, 278, 643, 694, 858, 1102 |
| NlaIV | GGNNCC | 3 cut(s) | 193, 253, 354 |
| NmeAIII | GCCGAG | 2 cut(s) | 36, 221 |
| NsiI | ATGCAT | 1 cut(s) | 856 |
| PaeR7I | CTCGAG | 1 cut(s) | 5 |
| PagI | TCATGA | 2 cut(s) | 264, 639 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 166 |
| PctI | GAATGC | 2 cut(s) | 720, 856 |
| PfeI | GAWTC | 4 cut(s) | 400, 482, 661, 1106 |
| PfoI | TCCNGGA | 1 cut(s) | 597 |
| PkrI | GCNGC | 1 cut(s) | 324 |
| PleI | GAGTC | 3 cut(s) | 86, 739, 886 |
| PluTI | GGCGCC | 1 cut(s) | 356 |
| PpsI | GAGTC | 3 cut(s) | 86, 739, 886 |
| Psp6I | CCWGG | 3 cut(s) | 597, 782, 994 |
| PspGI | CCWGG | 3 cut(s) | 597, 782, 994 |
| PspN4I | GGNNCC | 3 cut(s) | 193, 253, 354 |
| PspPI | GGNCC | 1 cut(s) | 191 |
| PspXI | VCTCGAGB | 1 cut(s) | 5 |
| PstI | CTGCAG | 1 cut(s) | 654 |
| PsuI | RGATCY | 1 cut(s) | 183 |
| RsaI | GTAC | 3 cut(s) | 33, 85, 583 |
| RsaNI | GTAC | 3 cut(s) | 32, 84, 582 |
| SaqAI | TTAA | 6 cut(s) | 506, 512, 623, 825, 1020, 1143 |
| SatI | GCNGC | 1 cut(s) | 323 |
| Sau3AI | GATC | 5 cut(s) | 183, 261, 465, 735, 921 |
| Sau96I | GGNCC | 1 cut(s) | 191 |
| ScaI | AGTACT | 1 cut(s) | 33 |
| SchI | GAGTC | 3 cut(s) | 86, 739, 887 |
| ScrFI | CCNGG | 3 cut(s) | 599, 784, 996 |
| SduI | GDGCHC | 2 cut(s) | 256, 783 |
| SfaNI | GCATC | 1 cut(s) | 637 |
| SfcI | CTRYAG | 1 cut(s) | 650 |
| SfoI | GGCGCC | 1 cut(s) | 354 |
| Sfr274I | CTCGAG | 1 cut(s) | 5 |
| SlaI | CTCGAG | 1 cut(s) | 5 |
| SmlI | CTYRAG | 2 cut(s) | 5, 511 |
| SmoI | CTYRAG | 2 cut(s) | 5, 511 |
| SpeI | ACTAGT | 1 cut(s) | 80 |
| Sse9I | AATT | 4 cut(s) | 331, 1004, 1057, 1064 |
| SsiI | CCGC | 1 cut(s) | 256 |
| SspDI | GGCGCC | 1 cut(s) | 352 |
| SspMI | CTAG | 5 cut(s) | 41, 81, 143, 776, 845 |
| StyD4I | CCNGG | 3 cut(s) | 597, 782, 994 |
| StyI | CCWWGG | 1 cut(s) | 187 |
| TaqI | TCGA | 5 cut(s) | 6, 169, 461, 468, 664 |
| TasI | AATT | 4 cut(s) | 331, 1004, 1057, 1064 |
| TatI | WGTACW | 2 cut(s) | 31, 83 |
| TfiI | GAWTC | 4 cut(s) | 400, 482, 661, 1106 |
| Tru1I | TTAA | 6 cut(s) | 506, 512, 623, 825, 1020, 1143 |
| Tru9I | TTAA | 6 cut(s) | 506, 512, 623, 825, 1020, 1143 |
| TscAI | CASTG | 2 cut(s) | 738, 1018 |
| TseI | GCWGC | 1 cut(s) | 322 |
| TspDTI | ATGAA | 6 cut(s) | 49, 187, 281, 395, 656, 1157 |
| TspGWI | ACGGA | 3 cut(s) | 710, 759, 1118 |
| TspRI | CASTG | 2 cut(s) | 738, 1018 |
| Vha464I | CTTAAG | 1 cut(s) | 511 |
| XhoI | CTCGAG | 1 cut(s) | 5 |
| XspI | CTAG | 5 cut(s) | 41, 81, 143, 776, 845 |
| ZrmI | AGTACT | 1 cut(s) | 33 |
| Zsp2I | ATGCAT | 1 cut(s) | 856 |