Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1551 |
| AarI | CACCTGC | 1 cut(s) | 815 |
| Acc36I | ACCTGC | 1 cut(s) | 815 |
| AccB1I | GGYRCC | 1 cut(s) | 673 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 143, 697, 1109 |
| AciI | CCGC | 6 cut(s) | 392, 651, 671, 707, 1406, 1748 |
| AclWI | GGATC | 4 cut(s) | 474, 637, 650, 1469 |
| AcoI | YGGCCR | 3 cut(s) | 433, 515, 793 |
| AcsI | RAATTY | 2 cut(s) | 955, 1648 |
| AcvI | CACGTG | 1 cut(s) | 887 |
| AcyI | GRCGYC | 1 cut(s) | 674 |
| AfaI | GTAC | 3 cut(s) | 687, 816, 1487 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 143, 539, 667, 697, 1109 |
| AgeI | ACCGGT | 1 cut(s) | 335 |
| AgsI | TTSAA | 5 cut(s) | 76, 848, 1042, 1327, 1402 |
| AjnI | CCWGG | 1 cut(s) | 943 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1266, 1298 |
| AluBI | AGCT | 7 cut(s) | 82, 770, 1048, 1316, 1352, 1721, 1781 |
| AluI | AGCT | 7 cut(s) | 82, 770, 1048, 1316, 1352, 1721, 1781 |
| Alw21I | GWGCWC | 2 cut(s) | 476, 726 |
| Alw26I | GTCTC | 3 cut(s) | 34, 1000, 1412 |
| Alw44I | GTGCAC | 1 cut(s) | 722 |
| AlwI | GGATC | 4 cut(s) | 474, 637, 650, 1469 |
| Ama87I | CYCGRG | 2 cut(s) | 31, 997 |
| AoxI | GGCC | 8 cut(s) | 343, 433, 515, 530, 541, 744, 793, 941 |
| ApaLI | GTGCAC | 1 cut(s) | 722 |
| ApoI | RAATTY | 2 cut(s) | 955, 1648 |
| AsiGI | ACCGGT | 1 cut(s) | 335 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1572 |
| AspLEI | GCGC | 1 cut(s) | 676 |
| AspS9I | GGNCC | 4 cut(s) | 479, 614, 636, 745 |
| AsuC2I | CCSGG | 1 cut(s) | 640 |
| AsuHPI | GGTGA | 3 cut(s) | 461, 839, 1420 |
| AvaI | CYCGRG | 2 cut(s) | 31, 997 |
| AvaII | GGWCC | 3 cut(s) | 479, 614, 636 |
| BaeGI | GKGCMC | 1 cut(s) | 726 |
| BalI | TGGCCA | 1 cut(s) | 795 |
| BamHI | GGATCC | 1 cut(s) | 642 |
| BanI | GGYRCC | 1 cut(s) | 673 |
| BbrPI | CACGTG | 1 cut(s) | 887 |
| BbsI | GAAGAC | 2 cut(s) | 901, 1365 |
| Bbv12I | GWGCWC | 2 cut(s) | 476, 726 |
| BccI | CCATC | 7 cut(s) | 59, 82, 661, 765, 1025, 1048, 1316 |
| BceAI | ACGGC | 5 cut(s) | 141, 502, 824, 1107, 1742 |
| BciT130I | CCWGG | 1 cut(s) | 945 |
| BclI | TGATCA | 2 cut(s) | 913, 1410 |
| BcnI | CCSGG | 1 cut(s) | 640 |
| BcoDI | GTCTC | 3 cut(s) | 34, 1000, 1412 |
| BfaI | CTAG | 5 cut(s) | 44, 414, 1010, 1458, 1643 |
| BfmI | CTRYAG | 2 cut(s) | 58, 1024 |
| BfoI | RGCGCY | 1 cut(s) | 677 |
| BfuAI | ACCTGC | 1 cut(s) | 815 |
| BisI | GCNGC | 2 cut(s) | 393, 672 |
| BlsI | GCNGC | 2 cut(s) | 394, 673 |
| BmcAI | AGTACT | 1 cut(s) | 816 |
| Bme1390I | CCNGG | 2 cut(s) | 640, 945 |
| Bme18I | GGWCC | 3 cut(s) | 479, 614, 636 |
| BmeT110I | CYCGRG | 2 cut(s) | 31, 997 |
| BmgT120I | GGNCC | 4 cut(s) | 479, 614, 636, 745 |
| BmiI | GGNNCC | 5 cut(s) | 155, 638, 644, 675, 1121 |
| BmrFI | CCNGG | 2 cut(s) | 640, 945 |
| BmsI | GCATC | 2 cut(s) | 957, 1380 |
| BpiI | GAAGAC | 2 cut(s) | 901, 1365 |
| BplI | GAGNNNNNCTC | 8 cut(s) | 25, 57, 531, 563, 601, 633, 991, 1023 |
| BpmI | CTGGAG | 2 cut(s) | 839, 1374 |
| BpuEI | CTTGAG | 4 cut(s) | 314, 533, 875, 1280 |
| BpuMI | CCSGG | 1 cut(s) | 640 |
| BsaAI | YACGTR | 1 cut(s) | 887 |
| BsaBI | GATNNNNATC | 1 cut(s) | 488 |
| BsaHI | GRCGYC | 1 cut(s) | 674 |
| BsaJI | CCNNGG | 4 cut(s) | 137, 533, 617, 1103 |
| BsaWI | WCCGGW | 1 cut(s) | 335 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 143, 539, 667, 697, 1109 |
| Bse118I | RCCGGY | 2 cut(s) | 335, 543 |
| Bse1I | ACTGG | 3 cut(s) | 253, 822, 1219 |
| Bse3DI | GCAATG | 1 cut(s) | 1673 |
| Bse8I | GATNNNNATC | 1 cut(s) | 488 |
| BseBI | CCWGG | 1 cut(s) | 945 |
| BseDI | CCNNGG | 4 cut(s) | 137, 533, 617, 1103 |
| BseGI | GGATG | 6 cut(s) | 247, 706, 1213, 1381, 1432, 1636 |
| BseJI | GATNNNNATC | 1 cut(s) | 488 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 143, 539, 667, 697, 1109 |
| BseMI | GCAATG | 1 cut(s) | 1673 |
| BseMII | CTCAG | 1 cut(s) | 1596 |
| BseNI | ACTGG | 3 cut(s) | 253, 822, 1219 |
| BseRI | GAGGAG | 1 cut(s) | 1517 |
| BseSI | GKGCMC | 1 cut(s) | 726 |
| Bsh1285I | CGRYCG | 1 cut(s) | 336 |
| BshFI | GGCC | 8 cut(s) | 345, 435, 517, 532, 543, 746, 795, 943 |
| BshNI | GGYRCC | 1 cut(s) | 673 |
| BshTI | ACCGGT | 1 cut(s) | 335 |
| BsiEI | CGRYCG | 1 cut(s) | 336 |
| BsiHKAI | GWGCWC | 2 cut(s) | 476, 726 |
| BsiHKCI | CYCGRG | 2 cut(s) | 31, 997 |
| BsiSI | CCGG | 4 cut(s) | 336, 436, 544, 640 |
| BsiWI | CGTACG | 1 cut(s) | 1485 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 143, 539, 667, 697, 1109 |
| BsmAI | GTCTC | 3 cut(s) | 34, 1000, 1412 |
| BsmBI | CGTCTC | 2 cut(s) | 34, 1000 |
| BsnI | GGCC | 8 cut(s) | 345, 435, 517, 532, 543, 746, 795, 943 |
| BsoBI | CYCGRG | 2 cut(s) | 31, 997 |
| Bsp1286I | GDGCHC | 2 cut(s) | 476, 726 |
| Bsp143I | GATC | 6 cut(s) | 466, 483, 642, 913, 1410, 1474 |
| Bsp19I | CCATGG | 2 cut(s) | 137, 1103 |
| BspACI | CCGC | 6 cut(s) | 392, 651, 671, 707, 1406, 1748 |
| BspANI | GGCC | 8 cut(s) | 345, 435, 517, 532, 543, 746, 795, 943 |
| BspCNI | CTCAG | 1 cut(s) | 1597 |
| BspLI | GGNNCC | 5 cut(s) | 155, 638, 644, 675, 1121 |
| BspMI | ACCTGC | 1 cut(s) | 815 |
| BspPI | GGATC | 4 cut(s) | 474, 637, 650, 1469 |
| BspQI | GCTCTTC | 1 cut(s) | 825 |
| BspT107I | GGYRCC | 1 cut(s) | 673 |
| BsrDI | GCAATG | 1 cut(s) | 1673 |
| BsrFI | RCCGGY | 2 cut(s) | 335, 543 |
| BsrI | ACTGG | 3 cut(s) | 253, 822, 1219 |
| BssAI | RCCGGY | 2 cut(s) | 335, 543 |
| BssECI | CCNNGG | 4 cut(s) | 137, 533, 617, 1103 |
| BssMI | GATC | 6 cut(s) | 466, 483, 642, 913, 1410, 1474 |
| BssNI | GRCGYC | 1 cut(s) | 674 |
| BssT1I | CCWWGG | 2 cut(s) | 137, 1103 |
| Bst2UI | CCWGG | 1 cut(s) | 945 |
| Bst4CI | ACNGT | 9 cut(s) | 62, 201, 568, 591, 728, 1028, 1167, 1531, 1538 |
| Bst6I | CTCTTC | 1 cut(s) | 825 |
| BstACI | GRCGYC | 1 cut(s) | 674 |
| BstBAI | YACGTR | 1 cut(s) | 887 |
| BstC8I | GCNNGC | 4 cut(s) | 318, 545, 744, 1588 |
| BstDEI | CTNAG | 2 cut(s) | 374, 1605 |
| BstDSI | CCRYGG | 2 cut(s) | 137, 1103 |
| BstF5I | GGATG | 6 cut(s) | 247, 706, 1213, 1381, 1432, 1636 |
| BstH2I | RGCGCY | 1 cut(s) | 677 |
| BstHHI | GCGC | 1 cut(s) | 676 |
| BstKTI | GATC | 6 cut(s) | 469, 486, 645, 916, 1413, 1477 |
| BstMAI | GTCTC | 3 cut(s) | 34, 1000, 1412 |
| BstMBI | GATC | 6 cut(s) | 466, 483, 642, 913, 1410, 1474 |
| BstMCI | CGRYCG | 1 cut(s) | 336 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 544 |
| BstNI | CCWGG | 1 cut(s) | 945 |
| BstSCI | CCNGG | 2 cut(s) | 638, 943 |
| BstSFI | CTRYAG | 2 cut(s) | 58, 1024 |
| BstSLI | GKGCMC | 1 cut(s) | 726 |
| BstV2I | GAAGAC | 2 cut(s) | 901, 1365 |
| BstX2I | RGATCY | 2 cut(s) | 642, 1474 |
| BstYI | RGATCY | 2 cut(s) | 642, 1474 |
| BsuRI | GGCC | 8 cut(s) | 345, 435, 517, 532, 543, 746, 795, 943 |
| BtgI | CCRYGG | 2 cut(s) | 137, 1103 |
| BtsCI | GGATG | 6 cut(s) | 247, 706, 1213, 1381, 1432, 1636 |
| BtsI | GCAGTG | 1 cut(s) | 1445 |
| BtsIMutI | CAGTG | 6 cut(s) | 67, 169, 1033, 1135, 1445, 1543 |
| BveI | ACCTGC | 1 cut(s) | 815 |
| Cac8I | GCNNGC | 4 cut(s) | 318, 545, 744, 1588 |
| CfoI | GCGC | 1 cut(s) | 676 |
| Cfr10I | RCCGGY | 2 cut(s) | 335, 543 |
| Cfr13I | GGNCC | 4 cut(s) | 479, 614, 636, 745 |
| Csp6I | GTAC | 3 cut(s) | 686, 815, 1486 |
| CspAI | ACCGGT | 1 cut(s) | 335 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 772, 807 |
| CviAII | CATG | 5 cut(s) | 138, 328, 911, 1104, 1708 |
| CviQI | GTAC | 3 cut(s) | 686, 815, 1486 |
| DdeI | CTNAG | 2 cut(s) | 374, 1605 |
| DinI | GGCGCC | 1 cut(s) | 675 |
| DpnI | GATC | 6 cut(s) | 468, 485, 644, 915, 1412, 1476 |
| DpnII | GATC | 6 cut(s) | 466, 483, 642, 913, 1410, 1474 |
| EaeI | YGGCCR | 3 cut(s) | 433, 515, 793 |
| Eam1104I | CTCTTC | 1 cut(s) | 825 |
| EarI | CTCTTC | 1 cut(s) | 825 |
| EciI | GGCGGA | 1 cut(s) | 722 |
| Eco130I | CCWWGG | 2 cut(s) | 137, 1103 |
| Eco47I | GGWCC | 3 cut(s) | 479, 614, 636 |
| Eco72I | CACGTG | 1 cut(s) | 887 |
| Eco88I | CYCGRG | 2 cut(s) | 31, 997 |
| EcoO109I | RGGNCCY | 1 cut(s) | 614 |
| EcoRII | CCWGG | 1 cut(s) | 943 |
| EcoT14I | CCWWGG | 2 cut(s) | 137, 1103 |
| EgeI | GGCGCC | 1 cut(s) | 675 |
| EheI | GGCGCC | 1 cut(s) | 675 |
| ErhI | CCWWGG | 2 cut(s) | 137, 1103 |
| Esp3I | CGTCTC | 2 cut(s) | 34, 1000 |
| FaeI | CATG | 5 cut(s) | 141, 331, 914, 1107, 1711 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 99, 131, 370, 402, 1065, 1097 |
| FatI | CATG | 5 cut(s) | 137, 327, 910, 1103, 1707 |
| FauI | CCCGC | 1 cut(s) | 1755 |
| FauNDI | CATATG | 3 cut(s) | 237, 837, 1203 |
| FbaI | TGATCA | 2 cut(s) | 913, 1410 |
| Fnu4HI | GCNGC | 2 cut(s) | 393, 672 |
| FokI | GGATG | 6 cut(s) | 254, 713, 1220, 1388, 1419, 1643 |
| Fsp4HI | GCNGC | 2 cut(s) | 393, 672 |
| FspBI | CTAG | 5 cut(s) | 44, 414, 1010, 1458, 1643 |
| GlaI | GCGC | 1 cut(s) | 675 |
| GluI | GCNGC | 2 cut(s) | 393, 672 |
| GsuI | CTGGAG | 2 cut(s) | 839, 1374 |
| HaeII | RGCGCY | 1 cut(s) | 677 |
| HaeIII | GGCC | 8 cut(s) | 345, 435, 517, 532, 543, 746, 795, 943 |
| HapII | CCGG | 4 cut(s) | 336, 436, 544, 640 |
| HhaI | GCGC | 1 cut(s) | 676 |
| Hin1I | GRCGYC | 1 cut(s) | 674 |
| Hin1II | CATG | 5 cut(s) | 141, 331, 914, 1107, 1711 |
| Hin6I | GCGC | 1 cut(s) | 674 |
| HinP1I | GCGC | 1 cut(s) | 674 |
| HincII | GTYRAC | 1 cut(s) | 1534 |
| HindII | GTYRAC | 1 cut(s) | 1534 |
| HindIII | AAGCTT | 1 cut(s) | 768 |
| HpaI | GTTAAC | 1 cut(s) | 1534 |
| HpaII | CCGG | 4 cut(s) | 336, 436, 544, 640 |
| HphI | GGTGA | 3 cut(s) | 461, 839, 1420 |
| Hpy166II | GTNNAC | 6 cut(s) | 283, 564, 724, 754, 1249, 1534 |
| Hpy188I | TCNGA | 3 cut(s) | 483, 488, 662 |
| Hpy8I | GTNNAC | 6 cut(s) | 283, 564, 724, 754, 1249, 1534 |
| Hpy99I | CGWCG | 1 cut(s) | 722 |
| HpyAV | CCTTC | 2 cut(s) | 433, 1396 |
| HpyCH4III | ACNGT | 9 cut(s) | 62, 201, 568, 591, 728, 1028, 1167, 1531, 1538 |
| HpyCH4IV | ACGT | 4 cut(s) | 285, 355, 886, 1251 |
| HpyCH4V | TGCA | 3 cut(s) | 724, 1393, 1657 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 544 |
| HpyF3I | CTNAG | 2 cut(s) | 374, 1605 |
| HpySE526I | ACGT | 4 cut(s) | 285, 355, 886, 1251 |
| Hsp92I | GRCGYC | 1 cut(s) | 674 |
| Hsp92II | CATG | 5 cut(s) | 141, 331, 914, 1107, 1711 |
| HspAI | GCGC | 1 cut(s) | 674 |
| KasI | GGCGCC | 1 cut(s) | 673 |
| KroI | GCCGGC | 1 cut(s) | 543 |
| KroNI | GCCGGC | 1 cut(s) | 545 |
| Ksp22I | TGATCA | 2 cut(s) | 913, 1410 |
| KspAI | GTTAAC | 1 cut(s) | 1534 |
| Kzo9I | GATC | 6 cut(s) | 466, 483, 642, 913, 1410, 1474 |
| LguI | GCTCTTC | 1 cut(s) | 825 |
| LmnI | GCTCC | 4 cut(s) | 91, 153, 1057, 1119 |
| LweI | GCATC | 2 cut(s) | 957, 1380 |
| MaeI | CTAG | 5 cut(s) | 44, 414, 1010, 1458, 1643 |
| MaeII | ACGT | 4 cut(s) | 285, 355, 886, 1251 |
| MaeIII | GTNAC | 6 cut(s) | 7, 251, 973, 1217, 1303, 1525 |
| MalI | GATC | 6 cut(s) | 468, 485, 644, 915, 1412, 1476 |
| MboI | GATC | 6 cut(s) | 466, 483, 642, 913, 1410, 1474 |
| MboII | GAAGA | 6 cut(s) | 521, 752, 842, 906, 1370, 1605 |
| MflI | RGATCY | 2 cut(s) | 642, 1474 |
| MhlI | GDGCHC | 2 cut(s) | 476, 726 |
| MlsI | TGGCCA | 1 cut(s) | 795 |
| MluCI | AATT | 8 cut(s) | 101, 179, 955, 1067, 1145, 1568, 1648, 1731 |
| MluNI | TGGCCA | 1 cut(s) | 795 |
| Mly113I | GGCGCC | 1 cut(s) | 674 |
| MlyI | GAGTC | 3 cut(s) | 167, 1133, 1414 |
| MmeI | TCCRAC | 1 cut(s) | 715 |
| Mox20I | TGGCCA | 1 cut(s) | 795 |
| MroNI | GCCGGC | 1 cut(s) | 543 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1572 |
| MscI | TGGCCA | 1 cut(s) | 795 |
| MseI | TTAA | 6 cut(s) | 959, 1344, 1367, 1533, 1752, 1783 |
| MslI | CAYNNNNRTG | 4 cut(s) | 240, 840, 909, 1206 |
| Msp20I | TGGCCA | 1 cut(s) | 795 |
| MspA1I | CMGCKG | 1 cut(s) | 1721 |
| MspI | CCGG | 4 cut(s) | 336, 436, 544, 640 |
| MspR9I | CCNGG | 2 cut(s) | 640, 945 |
| MvaI | CCWGG | 1 cut(s) | 945 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 544 |
| NaeI | GCCGGC | 1 cut(s) | 545 |
| NarI | GGCGCC | 1 cut(s) | 674 |
| NciI | CCSGG | 1 cut(s) | 640 |
| NcoI | CCATGG | 2 cut(s) | 137, 1103 |
| NdeI | CATATG | 3 cut(s) | 237, 837, 1203 |
| NdeII | GATC | 6 cut(s) | 466, 483, 642, 913, 1410, 1474 |
| NgoMIV | GCCGGC | 1 cut(s) | 543 |
| NlaIII | CATG | 5 cut(s) | 141, 331, 914, 1107, 1711 |
| NlaIV | GGNNCC | 5 cut(s) | 155, 638, 644, 675, 1121 |
| NmeAIII | GCCGAG | 1 cut(s) | 558 |
| NmuCI | GTSAC | 5 cut(s) | 7, 251, 973, 1217, 1525 |
| PaeR7I | CTCGAG | 2 cut(s) | 31, 997 |
| PaqCI | CACCTGC | 1 cut(s) | 815 |
| PciSI | GCTCTTC | 1 cut(s) | 825 |
| PdiI | GCCGGC | 1 cut(s) | 545 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1572 |
| Pfl23II | CGTACG | 1 cut(s) | 1485 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 143, 697, 1109 |
| PinAI | ACCGGT | 1 cut(s) | 335 |
| PkrI | GCNGC | 2 cut(s) | 394, 673 |
| PleI | GAGTC | 3 cut(s) | 167, 1133, 1414 |
| PluTI | GGCGCC | 1 cut(s) | 677 |
| PmaCI | CACGTG | 1 cut(s) | 887 |
| PmlI | CACGTG | 1 cut(s) | 887 |
| PpsI | GAGTC | 3 cut(s) | 167, 1133, 1414 |
| Ppu21I | YACGTR | 1 cut(s) | 887 |
| PpuMI | RGGWCCY | 1 cut(s) | 614 |
| PsiI | TTATAA | 1 cut(s) | 1551 |
| Psp5II | RGGWCCY | 1 cut(s) | 614 |
| Psp6I | CCWGG | 1 cut(s) | 943 |
| PspCI | CACGTG | 1 cut(s) | 887 |
| PspGI | CCWGG | 1 cut(s) | 943 |
| PspLI | CGTACG | 1 cut(s) | 1485 |
| PspN4I | GGNNCC | 5 cut(s) | 155, 638, 644, 675, 1121 |
| PspPI | GGNCC | 4 cut(s) | 479, 614, 636, 745 |
| PspPPI | RGGWCCY | 1 cut(s) | 614 |
| PsuI | RGATCY | 2 cut(s) | 642, 1474 |
| PvuII | CAGCTG | 1 cut(s) | 1721 |
| RsaI | GTAC | 3 cut(s) | 687, 816, 1487 |
| RsaNI | GTAC | 3 cut(s) | 686, 815, 1486 |
| RseI | CAYNNNNRTG | 4 cut(s) | 240, 840, 909, 1206 |
| SapI | GCTCTTC | 1 cut(s) | 825 |
| SaqAI | TTAA | 6 cut(s) | 959, 1344, 1367, 1533, 1752, 1783 |
| SatI | GCNGC | 2 cut(s) | 393, 672 |
| Sau3AI | GATC | 6 cut(s) | 466, 483, 642, 913, 1410, 1474 |
| Sau96I | GGNCC | 4 cut(s) | 479, 614, 636, 745 |
| ScaI | AGTACT | 1 cut(s) | 816 |
| SchI | GAGTC | 3 cut(s) | 167, 1133, 1414 |
| ScrFI | CCNGG | 2 cut(s) | 640, 945 |
| SduI | GDGCHC | 2 cut(s) | 476, 726 |
| SfaNI | GCATC | 2 cut(s) | 957, 1380 |
| SfcI | CTRYAG | 2 cut(s) | 58, 1024 |
| SfoI | GGCGCC | 1 cut(s) | 675 |
| Sfr274I | CTCGAG | 2 cut(s) | 31, 997 |
| SinI | GGWCC | 3 cut(s) | 479, 614, 636 |
| SlaI | CTCGAG | 2 cut(s) | 31, 997 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 240, 840, 909, 1206 |
| SmlI | CTYRAG | 6 cut(s) | 31, 293, 548, 854, 997, 1259 |
| SmoI | CTYRAG | 6 cut(s) | 31, 293, 548, 854, 997, 1259 |
| Sse9I | AATT | 8 cut(s) | 101, 179, 955, 1067, 1145, 1568, 1648, 1731 |
| SsiI | CCGC | 6 cut(s) | 392, 651, 671, 707, 1406, 1748 |
| SspDI | GGCGCC | 1 cut(s) | 673 |
| SspMI | CTAG | 5 cut(s) | 44, 414, 1010, 1458, 1643 |
| StyD4I | CCNGG | 2 cut(s) | 638, 943 |
| StyI | CCWWGG | 2 cut(s) | 137, 1103 |
| TaaI | ACNGT | 9 cut(s) | 62, 201, 568, 591, 728, 1028, 1167, 1531, 1538 |
| TaiI | ACGT | 4 cut(s) | 288, 358, 889, 1254 |
| TaqI | TCGA | 8 cut(s) | 32, 86, 176, 523, 998, 1052, 1142, 1509 |
| TaqII | GACCGA | 1 cut(s) | 862 |
| TasI | AATT | 8 cut(s) | 101, 179, 955, 1067, 1145, 1568, 1648, 1731 |
| TatI | WGTACW | 2 cut(s) | 685, 814 |
| TauI | GCSGC | 2 cut(s) | 395, 674 |
| Tru1I | TTAA | 6 cut(s) | 959, 1344, 1367, 1533, 1752, 1783 |
| Tru9I | TTAA | 6 cut(s) | 959, 1344, 1367, 1533, 1752, 1783 |
| TscAI | CASTG | 6 cut(s) | 67, 169, 1033, 1135, 1452, 1543 |
| TseFI | GTSAC | 5 cut(s) | 7, 251, 973, 1217, 1525 |
| Tsp45I | GTSAC | 5 cut(s) | 7, 251, 973, 1217, 1525 |
| TspDTI | ATGAA | 5 cut(s) | 282, 1248, 1344, 1724, 1724 |
| TspGWI | ACGGA | 3 cut(s) | 113, 1079, 1566 |
| TspRI | CASTG | 6 cut(s) | 67, 169, 1033, 1135, 1452, 1543 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 143, 697, 1109 |
| VneI | GTGCAC | 1 cut(s) | 722 |
| VpaK11BI | GGWCC | 3 cut(s) | 479, 614, 636 |
| XapI | RAATTY | 2 cut(s) | 955, 1648 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 144, 1110 |
| XhoI | CTCGAG | 2 cut(s) | 31, 997 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1572 |
| XspI | CTAG | 5 cut(s) | 44, 414, 1010, 1458, 1643 |
| ZrmI | AGTACT | 1 cut(s) | 816 |