RLG00000007418

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000693
Physical Location & Seq
Reverse (-)
16597438 .. 16597881
444 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007418

Sequence Viewer

Length: 360 bp
ATGCTTGCTCGAACTGAAGAGGGCGCTTGGGCCAAGCTTGAAGGGTTGGGCCAGGCCCGACCGTGGCTTCTCCTCACCCTCTTGTGCGGAGGAAGTGAAGGAGCGAGTTCGAAGGAGGTGGCCGCTCAGAGGGCGCGGCAGCGCCATCCCTTCTCCTCTTCAACTTCAAATCTAACAGCGGAGGACAACTCTCAGTCCGCTATGCGGCGCATCCAATGGTTGTCCATCCACCTGACGCCCGTCGGAATTTCACCGGACGATGACTATTGCATCCAATGGCTATCCAGCCCAGTGGTGCCGCATGGCTGTCTCGACCGCATAGACTCGGGCGTCGACCTCTGTGGACTTCTTCCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

120

Amino Acids

12.96

Weight (kDa)

5.49

Isoelectric Point (pI)

53.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 329
AccB1I GGYRCC 1 cut(s) 295
AccBSI CCGCTC 1 cut(s) 125
AccI GTMKAC 1 cut(s) 333
AccII CGCG 1 cut(s) 136
AciI CCGC 8 cut(s) 87, 123, 136, 179, 198, 205, 299, 316
AcoI YGGCCR 1 cut(s) 120
AcsI RAATTY 1 cut(s) 246
AcuI CTGAAG 1 cut(s) 36
AcyI GRCGYC 2 cut(s) 236, 330
AfiI CCNNNNNNNGG 3 cut(s) 63, 129, 204
AgsI TTSAA 3 cut(s) 41, 162, 168
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 1 cut(s) 37
AluI AGCT 1 cut(s) 37
Alw26I GTCTC 1 cut(s) 314
Ama87I CYCGRG 1 cut(s) 325
AoxI GGCC 4 cut(s) 30, 49, 54, 120
ApeKI GCWGC 1 cut(s) 139
ApoI RAATTY 1 cut(s) 246
AspLEI GCGC 4 cut(s) 26, 136, 144, 210
AspS9I GGNCC 3 cut(s) 30, 49, 55
AsuHPI GGTGA 2 cut(s) 67, 243
AsuII TTCGAA 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 325
BanI GGYRCC 1 cut(s) 295
BbvI GCAGC 1 cut(s) 151
BccI CCATC 2 cut(s) 153, 233
BciT130I CCWGG 1 cut(s) 53
BcoDI GTCTC 1 cut(s) 314
BfoI RGCGCY 2 cut(s) 27, 145
BisI GCNGC 5 cut(s) 123, 137, 140, 206, 299
BlsI GCNGC 5 cut(s) 124, 138, 141, 207, 300
Bme1390I CCNGG 1 cut(s) 53
BmeT110I CYCGRG 1 cut(s) 325
BmgT120I GGNCC 3 cut(s) 30, 49, 55
BmiI GGNNCC 1 cut(s) 297
BmrFI CCNGG 1 cut(s) 53
BmrI ACTGGG 1 cut(s) 284
BmsI GCATC 2 cut(s) 219, 279
BmuI ACTGGG 1 cut(s) 284
BoxI GACNNNNGTC 1 cut(s) 239
BplI GAGNNNNNCTC 2 cut(s) 173, 205
Bpu14I TTCGAA 1 cut(s) 110
BsaHI GRCGYC 2 cut(s) 236, 330
BsaJI CCNNGG 1 cut(s) 62
BsaWI WCCGGW 1 cut(s) 253
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 129, 204
Bse1I ACTGG 1 cut(s) 290
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 1 cut(s) 62
BseGI GGATG 4 cut(s) 145, 210, 225, 270
BseLI CCNNNNNNNGG 3 cut(s) 63, 129, 204
BseMII CTCAG 2 cut(s) 140, 206
BseNI ACTGG 1 cut(s) 290
BseRI GAGGAG 2 cut(s) 62, 145
BseXI GCAGC 1 cut(s) 151
Bsh1236I CGCG 1 cut(s) 136
Bsh1285I CGRYCG 2 cut(s) 62, 316
BshFI GGCC 4 cut(s) 32, 51, 56, 122
BshNI GGYRCC 1 cut(s) 295
BsiEI CGRYCG 2 cut(s) 62, 316
BsiHKCI CYCGRG 1 cut(s) 325
BsiSI CCGG 1 cut(s) 254
BslI CCNNNNNNNGG 3 cut(s) 63, 129, 204
BsmAI GTCTC 1 cut(s) 314
BsnI GGCC 4 cut(s) 32, 51, 56, 122
BsoBI CYCGRG 1 cut(s) 325
Bsp119I TTCGAA 1 cut(s) 110
BspACI CCGC 8 cut(s) 87, 123, 136, 179, 198, 205, 299, 316
BspANI GGCC 4 cut(s) 32, 51, 56, 122
BspCNI CTCAG 2 cut(s) 139, 205
BspFNI CGCG 1 cut(s) 136
BspLI GGNNCC 1 cut(s) 297
BspT104I TTCGAA 1 cut(s) 110
BspT107I GGYRCC 1 cut(s) 295
BsrBI CCGCTC 1 cut(s) 125
BsrI ACTGG 1 cut(s) 290
BssECI CCNNGG 1 cut(s) 62
BssNI GRCGYC 2 cut(s) 236, 330
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 1 cut(s) 63
Bst6I CTCTTC 2 cut(s) 12, 163
BstACI GRCGYC 2 cut(s) 236, 330
BstBI TTCGAA 1 cut(s) 110
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 2 cut(s) 126, 192
BstDSI CCRYGG 1 cut(s) 62
BstF5I GGATG 4 cut(s) 145, 210, 225, 270
BstFNI CGCG 1 cut(s) 136
BstH2I RGCGCY 2 cut(s) 27, 145
BstHHI GCGC 4 cut(s) 26, 136, 144, 210
BstMAI GTCTC 1 cut(s) 314
BstMCI CGRYCG 2 cut(s) 62, 316
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNI CCWGG 1 cut(s) 53
BstPAI GACNNNNGTC 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 51
BstUI CGCG 1 cut(s) 136
BstV1I GCAGC 1 cut(s) 151
BstXI CCANNNNNNTGG 1 cut(s) 292
BsuRI GGCC 4 cut(s) 32, 51, 56, 122
BtgI CCRYGG 1 cut(s) 62
BtsCI GGATG 4 cut(s) 145, 210, 225, 270
BtsIMutI CAGTG 1 cut(s) 297
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 4 cut(s) 26, 136, 144, 210
Cfr13I GGNCC 3 cut(s) 30, 49, 55
CseI GACGC 2 cut(s) 244, 319
CviAII CATG 1 cut(s) 302
CviJI RGCY 9 cut(s) 32, 37, 51, 56, 67, 122, 280, 288, 306
CviKI_1 RGCY 9 cut(s) 32, 37, 51, 56, 67, 122, 280, 288, 306
DdeI CTNAG 2 cut(s) 126, 192
DrdI GACNNNNNNGTC 1 cut(s) 329
DseDI GACNNNNNNGTC 1 cut(s) 329
EaeI YGGCCR 1 cut(s) 120
Eam1104I CTCTTC 2 cut(s) 12, 163
EarI CTCTTC 2 cut(s) 12, 163
Eco57I CTGAAG 1 cut(s) 36
Eco88I CYCGRG 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 51
FaeI CATG 1 cut(s) 305
FaiI YATR 3 cut(s) 203, 303, 320
FatI CATG 1 cut(s) 301
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 5 cut(s) 123, 137, 140, 206, 299
FokI GGATG 4 cut(s) 132, 197, 212, 257
Fsp4HI GCNGC 5 cut(s) 123, 137, 140, 206, 299
GlaI GCGC 4 cut(s) 25, 135, 143, 209
GluI GCNGC 5 cut(s) 123, 137, 140, 206, 299
HaeII RGCGCY 2 cut(s) 27, 145
HaeIII GGCC 4 cut(s) 32, 51, 56, 122
HapII CCGG 1 cut(s) 254
HgaI GACGC 2 cut(s) 244, 319
HhaI GCGC 4 cut(s) 26, 136, 144, 210
Hin1I GRCGYC 2 cut(s) 236, 330
Hin1II CATG 1 cut(s) 305
Hin6I GCGC 4 cut(s) 24, 134, 142, 208
HinP1I GCGC 4 cut(s) 24, 134, 142, 208
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 1 cut(s) 323
HpaII CCGG 1 cut(s) 254
HphI GGTGA 2 cut(s) 67, 243
Hpy166II GTNNAC 2 cut(s) 334, 344
Hpy188I TCNGA 2 cut(s) 129, 245
Hpy188III TCNNGA 1 cut(s) 311
Hpy8I GTNNAC 2 cut(s) 334, 344
Hpy99I CGWCG 2 cut(s) 245, 335
HpyAV CCTTC 4 cut(s) 35, 92, 106, 160
HpyCH4III ACNGT 1 cut(s) 63
HpyCH4V TGCA 1 cut(s) 270
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 2 cut(s) 126, 192
Hsp92I GRCGYC 2 cut(s) 236, 330
Hsp92II CATG 1 cut(s) 305
HspAI GCGC 4 cut(s) 24, 134, 142, 208
LmnI GCTCC 1 cut(s) 101
LpnPI CCDG 6 cut(s) 38, 65, 245, 267, 298, 303
Lsp1109I GCAGC 1 cut(s) 151
LweI GCATC 2 cut(s) 219, 279
MbiI CCGCTC 1 cut(s) 125
MboII GAAGA 3 cut(s) 29, 150, 341
MluCI AATT 1 cut(s) 246
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 9 cut(s) 13, 83, 83, 89, 109, 123, 166, 175, 347
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 53
MvaI CCWGG 1 cut(s) 53
MvnI CGCG 1 cut(s) 136
MwoI GCNNNNNNNGC 1 cut(s) 131
NlaIII CATG 1 cut(s) 305
NlaIV GGNNCC 1 cut(s) 297
NspV TTCGAA 1 cut(s) 110
PkrI GCNGC 5 cut(s) 124, 138, 141, 207, 300
PleI GAGTC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 317
PshAI GACNNNNGTC 1 cut(s) 239
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 297
PspPI GGNCC 3 cut(s) 30, 49, 55
SalI GTCGAC 1 cut(s) 332
SatI GCNGC 5 cut(s) 123, 137, 140, 206, 299
Sau96I GGNCC 3 cut(s) 30, 49, 55
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 53
SetI ASST 4 cut(s) 39, 120, 234, 339
SfaNI GCATC 2 cut(s) 219, 279
SfuI TTCGAA 1 cut(s) 110
Sse9I AATT 1 cut(s) 246
SsiI CCGC 8 cut(s) 87, 123, 136, 179, 198, 205, 299, 316
StyD4I CCNGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 63
TaqI TCGA 4 cut(s) 10, 110, 312, 333
TasI AATT 1 cut(s) 246
TauI GCSGC 4 cut(s) 125, 139, 208, 301
TscAI CASTG 1 cut(s) 297
TseI GCWGC 1 cut(s) 139
TspRI CASTG 1 cut(s) 297
XapI RAATTY 1 cut(s) 246
XmiI GTMKAC 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.