Rroxscaffold_2G00145640

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000693
Physical Location & Seq
Forward (+)
83521371 .. 83524165
2795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00145640.1

Sequence Viewer

Length: 420 bp
ATGCCTTACTGCTGTCAAGCAACAAAGCAGAAGACTTGGTTCAATTTCTGGGTTCTTCCTTCAAGCTCAATTCACTGGGAGGAAGAAGCTGGTGACATACTTGTCTTCTTGACGGGTAAAGACGAAATTGAATTCGTAGAAAGACTTATTGAAGAAAAACTTAAACAGTTACCGGAGAATAGTCAAAAACTAGTAGCGGTACCTTTATTTTCATCTCTTCCTTCAGAACAGCAAATGGGAGCTTTTGCACCAGCCTGTCCTAGGTTTCGTAAGGTAGTTTTGGCAACCAATATTGCTGAGACGTCAGTGACAATACCTGGAATCAAGTATGTTATAGACACTGGGGTTGTAAAAGCACACTTCTATAATGCAAACAAAGGGCTGGAACCTTTGATTGTTGTTCCAATTTCGAAAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0001817 GO:0001819 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003723 GO:0003724 GO:0003725 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006356 GO:0006396 GO:0006412 GO:0006413 GO:0006518 GO:0006725 GO:0006807 GO:0008026 GO:0008134 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023056 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032479 GO:0032481 GO:0032991 GO:0033613 GO:0034641 GO:0034645 GO:0042325 GO:0042327 GO:0042623 GO:0043021 GO:0043023 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043408 GO:0043410 GO:0043484 GO:0043565 GO:0043603 GO:0043604 GO:0044087 GO:0044089 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045937 GO:0045943 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051090 GO:0051091 GO:0051092 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0060255 GO:0061702 GO:0065007 GO:0065009 GO:0070013 GO:0070035 GO:0071704 GO:0072559 GO:0080090 GO:0090304 GO:0097159 GO:0140098 GO:1900225 GO:1900227 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 ko03050 ko05169 map03040 map03050 map05169
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

139

Amino Acids

15.57

Weight (kDa)

6.12

Isoelectric Point (pI)

54.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 26 - 116 4.9e-08 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 101
AatII GACGTC 1 cut(s) 305
Acc65I GGTACC 1 cut(s) 199
AccB1I GGYRCC 1 cut(s) 199
AciI CCGC 1 cut(s) 197
AcsI RAATTY 1 cut(s) 131
AcuI CTGAAG 1 cut(s) 207
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 1 cut(s) 201
AfiI CCNNNNNNNGG 1 cut(s) 261
AgsI TTSAA 4 cut(s) 43, 63, 131, 152
AhlI ACTAGT 1 cut(s) 190
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 3 cut(s) 66, 89, 242
AluI AGCT 3 cut(s) 66, 89, 242
Alw26I GTCTC 1 cut(s) 293
ApoI RAATTY 1 cut(s) 131
Asp718I GGTACC 1 cut(s) 199
AspA2I CCTAGG 1 cut(s) 260
AsuHPI GGTGA 1 cut(s) 104
AsuII TTCGAA 1 cut(s) 410
AvrII CCTAGG 1 cut(s) 260
BanI GGYRCC 1 cut(s) 199
BbsI GAAGAC 2 cut(s) 38, 97
BciT130I CCWGG 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 293
BcuI ACTAGT 1 cut(s) 190
BfaI CTAG 2 cut(s) 191, 261
BlnI CCTAGG 1 cut(s) 260
Bme1390I CCNGG 1 cut(s) 318
BmiI GGNNCC 2 cut(s) 201, 387
BmrFI CCNGG 1 cut(s) 318
BmrI ACTGGG 2 cut(s) 85, 351
BmuI ACTGGG 2 cut(s) 85, 351
BpiI GAAGAC 2 cut(s) 38, 97
Bpu14I TTCGAA 1 cut(s) 410
BsaHI GRCGYC 1 cut(s) 302
BsaJI CCNNGG 1 cut(s) 260
BsaWI WCCGGW 1 cut(s) 172
Bsc4I CCNNNNNNNGG 1 cut(s) 261
Bse1I ACTGG 2 cut(s) 80, 346
BseBI CCWGG 1 cut(s) 318
BseDI CCNNGG 1 cut(s) 260
BseLI CCNNNNNNNGG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 288
BseNI ACTGG 2 cut(s) 80, 346
BshNI GGYRCC 1 cut(s) 199
BsiSI CCGG 1 cut(s) 173
BslI CCNNNNNNNGG 1 cut(s) 261
BsmAI GTCTC 1 cut(s) 293
BsmBI CGTCTC 1 cut(s) 293
Bsp119I TTCGAA 1 cut(s) 410
BspACI CCGC 1 cut(s) 197
BspCNI CTCAG 1 cut(s) 289
BspLI GGNNCC 2 cut(s) 201, 387
BspT104I TTCGAA 1 cut(s) 410
BspT107I GGYRCC 1 cut(s) 199
BsrI ACTGG 2 cut(s) 80, 346
BssECI CCNNGG 1 cut(s) 260
BssNI GRCGYC 1 cut(s) 302
BssT1I CCWWGG 1 cut(s) 260
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 1 cut(s) 168
Bst6I CTCTTC 1 cut(s) 222
BstACI GRCGYC 1 cut(s) 302
BstBI TTCGAA 1 cut(s) 410
BstDEI CTNAG 1 cut(s) 297
BstENI CCTNNNNNAGG 1 cut(s) 259
BstMAI GTCTC 1 cut(s) 293
BstNI CCWGG 1 cut(s) 318
BstSCI CCNGG 1 cut(s) 316
BstV2I GAAGAC 2 cut(s) 38, 97
BtsIMutI CAGTG 3 cut(s) 73, 312, 339
Csp6I GTAC 1 cut(s) 200
CviJI RGCY 5 cut(s) 66, 89, 242, 254, 382
CviKI_1 RGCY 5 cut(s) 66, 89, 242, 254, 382
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 1 cut(s) 297
DrdI GACNNNNNNGTC 1 cut(s) 101
DseDI GACNNNNNNGTC 1 cut(s) 101
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
Eco130I CCWWGG 1 cut(s) 260
Eco57I CTGAAG 1 cut(s) 207
EcoNI CCTNNNNNAGG 1 cut(s) 259
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 316
EcoT14I CCWWGG 1 cut(s) 260
ErhI CCWWGG 1 cut(s) 260
Esp3I CGTCTC 1 cut(s) 293
FaiI YATR 5 cut(s) 98, 330, 335, 366, 418
FalI AAGNNNNNCTT 2 cut(s) 144, 176
FspBI CTAG 2 cut(s) 191, 261
HapII CCGG 1 cut(s) 173
Hin1I GRCGYC 1 cut(s) 302
HinfI GANTC 1 cut(s) 321
HpaII CCGG 1 cut(s) 173
HphI GGTGA 1 cut(s) 104
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 1 cut(s) 109
HpyAV CCTTC 2 cut(s) 69, 231
HpyCH4III ACNGT 1 cut(s) 168
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 2 cut(s) 248, 371
HpyF3I CTNAG 1 cut(s) 297
HpySE526I ACGT 1 cut(s) 302
Hsp92I GRCGYC 1 cut(s) 302
KpnI GGTACC 1 cut(s) 203
LmnI GCTCC 1 cut(s) 239
MaeI CTAG 2 cut(s) 191, 261
MaeII ACGT 1 cut(s) 302
MaeIII GTNAC 3 cut(s) 92, 168, 307
MboII GAAGA 6 cut(s) 43, 47, 95, 97, 164, 209
MluCI AATT 5 cut(s) 43, 69, 126, 131, 405
MnlI CCTC 1 cut(s) 73
MseI TTAA 1 cut(s) 162
MspI CCGG 1 cut(s) 173
MspR9I CCNGG 1 cut(s) 318
MvaI CCWGG 1 cut(s) 318
NlaIV GGNNCC 2 cut(s) 201, 387
NmuCI GTSAC 2 cut(s) 92, 307
NspV TTCGAA 1 cut(s) 410
PfeI GAWTC 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 316
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 2 cut(s) 201, 387
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
SaqAI TTAA 1 cut(s) 162
ScrFI CCNGG 1 cut(s) 318
SetI ASST 9 cut(s) 68, 91, 205, 244, 266, 276, 305, 319, 391
SfuI TTCGAA 1 cut(s) 410
SpeI ACTAGT 1 cut(s) 190
Sse9I AATT 5 cut(s) 43, 69, 126, 131, 405
SsiI CCGC 1 cut(s) 197
SspI AATATT 1 cut(s) 292
SspMI CTAG 2 cut(s) 191, 261
StyD4I CCNGG 1 cut(s) 316
StyI CCWWGG 1 cut(s) 260
TaaI ACNGT 1 cut(s) 168
TaiI ACGT 1 cut(s) 305
TaqI TCGA 1 cut(s) 410
TasI AATT 5 cut(s) 43, 69, 126, 131, 405
TfiI GAWTC 1 cut(s) 321
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 3 cut(s) 80, 312, 346
TseFI GTSAC 2 cut(s) 92, 307
Tsp45I GTSAC 2 cut(s) 92, 307
TspDTI ATGAA 1 cut(s) 201
TspRI CASTG 3 cut(s) 80, 312, 346
XagI CCTNNNNNAGG 1 cut(s) 259
XapI RAATTY 1 cut(s) 131
XmaJI CCTAGG 1 cut(s) 260
XspI CTAG 2 cut(s) 191, 261
ZraI GACGTC 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.