RLG00000032135

WPP domain-interacting tail-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000372
Physical Location & Seq
Reverse (-)
11301762 .. 11307113
5352 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032135

Sequence Viewer

Length: 684 bp
ATGGCGGAGGCTCTTAATTACATTCAGGTACAGGTATTTCTGAGAGATTTTGTTCCTACTGGTTTGGCCTGCCTTTCAGATGGCTTTGTTCCTATTGGTTTGGCTTTTGTTTTTAGTATTCTTCAAGTAGTTCCAAAGTTGGAGATAGGCTACACACATCAATGGATGTCAGTTAGTGAATCCAGCGCTCAAGAGTTGGAGAGTTCCATGAAAGTTTTGACAAAAGTGGACTTGGACTTGGCATATTCTGCTGAGAAGTTGGCAAATCTACAGGAGCTTATGATGCGCCTATTCTATCAAGAAAATGATCTTGAAGCAATGGCCACCGCAAATAACTATATCATGAGAAACTATTTTCATTTTTTCTTCAACTGTAAAACCTACACTTACCTTGATGGTGAGGTCGAGAGGGAGAGCCCAAGCGACGGAGTTGTGCTTAGAGTCTCGTCATCTTGCCGAGCGCTTACCGGCCTTAAGGAGCACCGATCCCAACTGCAGGGCGCGATCAATGAGCTTGGTTTGATCGGCGACGGAGTCACGGGAGTCGAGGATCCGTTCGAAGGAGAGATTGATGGCGAAGGGCGGCGAGTCAGAGAAGTCAAGGAAGGTCTCCAGGAGGTGGTCACGGTCAAGGAGCATGACGTGATAAGGGTTTGGAGGGGGAGAGAGAGAGACAGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008360 GO:0022603 GO:0022604 GO:0032878 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0065007 GO:0065008 GO:2000769
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

228

Amino Acids

25.74

Weight (kDa)

4.83

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WIT1_2_N PF26581 66 - 120 2.7e-15 WIT1/2, N-terminal helical bundle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000372)

Species Orthologous Gene IDs
pyrus_communis pycom04g09000
rosa_chinensis RchiOBHm_Chr1g0319921 RchiOBHm_Chr2g0124671 RchiOBHm_Chr3g0488631 RchiOBHm_Chr3g0495191 RchiOBHm_Chr5g0067451 RchiOBHm_Chr6g0269581 RchiOBHm_Chr7g0210411 RchiOBHm_Chr7g0212341 RchiOBHm_Chr7g0214191
rosa_laevigata RLG00000003882 RLG00000008030 RLG00000011591 RLG00000016235 RLG00000018631 RLG00000031951 RLG00000032135 RLG00000032548 RLG00000033239
rosa_multiflora Rmu_sc0000281.1_g000015 Rmu_sc0000516.1_g000020 Rmu_sc0000816.1_g000025 Rmu_sc0001850.1_g000002 Rmu_sc0003260.1_g000025 Rmu_sc0007581.1_g000007 Rmu_sc0009241.1_g000001
rosa_roxburghii Rroxscaffold_3G00218290 Rroxscaffold_3G00240930 Rroxscaffold_4G00300430 Rroxscaffold_5G00334810 Rroxscaffold_5G00360330 Rroxscaffold_7G00180190 Rroxscaffold_7G00192890
rosa_rugosa Rorug01G0357800 Rorug01G0357900 Rorug03G0350400 Rorug05G0174000 Rorug05G0231100 Rorug05G0231100 Rorug05G0231200 Rorug05G0231200 Rorug05G0577100 Rorug07G0029800 Rorug07G0029900 Rorug07G0146200
rosa_samantha Rh1AG039300 Rh1AG155100 Rh1AG173000 Rh1AG200900 Rh1AG276400 Rh1BG055400 Rh1BG111700 Rh1CG081000 Rh1CG161100 Rh1CG161200 Rh1CG247000 Rh1CG264000 Rh1DG031600 Rh1DG163400 Rh2AG236000 Rh2AG236100 Rh2AG277400 Rh2AG277500 Rh2AG309700 Rh2AG339600 Rh2BG221000 Rh2DG243300 Rh2DG243400 Rh2DG267900 Rh2DG434000 Rh3AG280300 Rh3AG288700 Rh3CG224100 Rh4AG043200 Rh4AG260400 Rh4BG266100 Rh4CG071300 Rh4DG155900 Rh5AG213100 Rh5AG238500 Rh5AG238600 Rh5AG296300 Rh5AG463800 Rh5CG236400 Rh5CG269500 Rh5CG269600 Rh5DG131600 Rh6AG074700 Rh6AG077500 Rh6AG093000 Rh6AG286000 Rh6CG246600 Rh7AG171100 Rh7AG303600 Rh7AG389400 Rh7AG390000 Rh7AG446200 Rh7CG251500 Rh7CG408700 Rh7DG385600
rosa_wichuraiana Rw0G014360 Rw0G015950 Rw2G017860 Rw2G027110 Rw4G037790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 619
AccII CGCG 1 cut(s) 503
AciI CCGC 3 cut(s) 5, 327, 583
AclWI GGATC 3 cut(s) 480, 545, 558
AcoI YGGCCR 1 cut(s) 321
AfaI GTAC 1 cut(s) 30
AfeI AGCGCT 2 cut(s) 187, 462
AfiI CCNNNNNNNGG 4 cut(s) 425, 496, 560, 619
AflII CTTAAG 1 cut(s) 473
AgsI TTSAA 3 cut(s) 125, 314, 370
AjiI CACGTC 1 cut(s) 643
AjnI CCWGG 1 cut(s) 612
AluBI AGCT 2 cut(s) 277, 514
AluI AGCT 2 cut(s) 277, 514
Alw21I GWGCWC 1 cut(s) 483
Alw26I GTCTC 3 cut(s) 448, 614, 666
AlwI GGATC 3 cut(s) 480, 545, 558
Aor51HI AGCGCT 2 cut(s) 187, 462
AoxI GGCC 3 cut(s) 66, 321, 469
AspLEI GCGC 4 cut(s) 188, 288, 463, 503
AsuHPI GGTGA 1 cut(s) 410
AsuII TTCGAA 1 cut(s) 558
BalI TGGCCA 1 cut(s) 323
BamHI GGATCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 483
BccI CCATC 3 cut(s) 74, 389, 566
BciT130I CCWGG 1 cut(s) 614
BcoDI GTCTC 3 cut(s) 448, 614, 666
BfmI CTRYAG 2 cut(s) 269, 494
BfoI RGCGCY 2 cut(s) 189, 464
BfrI CTTAAG 1 cut(s) 473
BisI GCNGC 1 cut(s) 584
BlsI GCNGC 1 cut(s) 585
Bme1390I CCNGG 1 cut(s) 614
BmgBI CACGTC 1 cut(s) 643
BmiI GGNNCC 1 cut(s) 552
BmrFI CCNGG 1 cut(s) 614
BmsI GCATC 1 cut(s) 273
BpmI CTGGAG 1 cut(s) 596
Bpu14I TTCGAA 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 614
Bsc4I CCNNNNNNNGG 4 cut(s) 425, 496, 560, 619
Bse118I RCCGGY 1 cut(s) 467
Bse1I ACTGG 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 324
BseBI CCWGG 1 cut(s) 614
BseGI GGATG 1 cut(s) 171
BseLI CCNNNNNNNGG 4 cut(s) 425, 496, 560, 619
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 2 cut(s) 32, 243
BseNI ACTGG 1 cut(s) 64
Bsh1236I CGCG 1 cut(s) 503
BshFI GGCC 3 cut(s) 68, 323, 471
BsiHKAI GWGCWC 1 cut(s) 483
BsiSI CCGG 1 cut(s) 468
BslI CCNNNNNNNGG 4 cut(s) 425, 496, 560, 619
BsmAI GTCTC 3 cut(s) 448, 614, 666
BsnI GGCC 3 cut(s) 68, 323, 471
Bso31I GGTCTC 1 cut(s) 614
Bsp119I TTCGAA 1 cut(s) 558
Bsp1286I GDGCHC 2 cut(s) 419, 483
Bsp143I GATC 5 cut(s) 307, 485, 504, 522, 550
BspACI CCGC 3 cut(s) 5, 327, 583
BspANI GGCC 3 cut(s) 68, 323, 471
BspCNI CTCAG 2 cut(s) 33, 244
BspFNI CGCG 1 cut(s) 503
BspHI TCATGA 1 cut(s) 342
BspLI GGNNCC 1 cut(s) 552
BspMAI CTGCAG 1 cut(s) 498
BspPI GGATC 3 cut(s) 480, 545, 558
BspT104I TTCGAA 1 cut(s) 558
BspTI CTTAAG 1 cut(s) 473
BspTNI GGTCTC 1 cut(s) 614
BsrDI GCAATG 1 cut(s) 324
BsrFI RCCGGY 1 cut(s) 467
BsrI ACTGG 1 cut(s) 64
BssAI RCCGGY 1 cut(s) 467
BssMI GATC 5 cut(s) 307, 485, 504, 522, 550
Bst2UI CCWGG 1 cut(s) 614
Bst4CI ACNGT 2 cut(s) 374, 628
BstAFI CTTAAG 1 cut(s) 473
BstAPI GCANNNNNTGC 1 cut(s) 248
BstBI TTCGAA 1 cut(s) 558
BstC8I GCNNGC 1 cut(s) 70
BstDEI CTNAG 3 cut(s) 41, 252, 437
BstF5I GGATG 1 cut(s) 171
BstFNI CGCG 1 cut(s) 503
BstH2I RGCGCY 2 cut(s) 189, 464
BstHHI GCGC 4 cut(s) 188, 288, 463, 503
BstKTI GATC 5 cut(s) 310, 488, 507, 525, 553
BstMAI GTCTC 3 cut(s) 448, 614, 666
BstMBI GATC 5 cut(s) 307, 485, 504, 522, 550
BstMWI GCNNNNNNNGC 2 cut(s) 248, 283
BstNI CCWGG 1 cut(s) 614
BstSCI CCNGG 1 cut(s) 612
BstSFI CTRYAG 2 cut(s) 269, 494
BstUI CGCG 1 cut(s) 503
BstX2I RGATCY 1 cut(s) 550
BstYI RGATCY 1 cut(s) 550
BsuRI GGCC 3 cut(s) 68, 323, 471
BtrI CACGTC 1 cut(s) 643
BtsCI GGATG 1 cut(s) 171
Cac8I GCNNGC 1 cut(s) 70
CciI TCATGA 1 cut(s) 342
CfoI GCGC 4 cut(s) 188, 288, 463, 503
Cfr10I RCCGGY 1 cut(s) 467
Csp6I GTAC 1 cut(s) 29
CviAII CATG 3 cut(s) 208, 343, 638
CviQI GTAC 1 cut(s) 29
DdeI CTNAG 3 cut(s) 41, 252, 437
DpnI GATC 5 cut(s) 309, 487, 506, 524, 552
DpnII GATC 5 cut(s) 307, 485, 504, 522, 550
EaeI YGGCCR 1 cut(s) 321
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 419
Eco31I GGTCTC 1 cut(s) 614
Eco47III AGCGCT 2 cut(s) 187, 462
EcoRII CCWGG 1 cut(s) 612
EcoT38I GRGCYC 1 cut(s) 419
FaeI CATG 3 cut(s) 211, 346, 641
FaiI YATR 6 cut(s) 209, 244, 281, 339, 344, 639
FatI CATG 3 cut(s) 207, 342, 637
Fnu4HI GCNGC 1 cut(s) 584
FokI GGATG 1 cut(s) 178
FriOI GRGCYC 1 cut(s) 419
Fsp4HI GCNGC 1 cut(s) 584
GlaI GCGC 4 cut(s) 187, 287, 462, 502
GluI GCNGC 1 cut(s) 584
GsuI CTGGAG 1 cut(s) 596
HaeII RGCGCY 2 cut(s) 189, 464
HaeIII GGCC 3 cut(s) 68, 323, 471
HapII CCGG 1 cut(s) 468
HhaI GCGC 4 cut(s) 188, 288, 463, 503
Hin1II CATG 3 cut(s) 211, 346, 641
Hin6I GCGC 4 cut(s) 186, 286, 461, 501
HinP1I GCGC 4 cut(s) 186, 286, 461, 501
HinfI GANTC 5 cut(s) 179, 441, 534, 543, 588
HpaII CCGG 1 cut(s) 468
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 3 cut(s) 42, 79, 593
Hpy188III TCNNGA 5 cut(s) 191, 299, 311, 343, 406
Hpy8I GTNNAC 1 cut(s) 229
Hpy99I CGWCG 2 cut(s) 428, 533
HpyAV CCTTC 3 cut(s) 554, 572, 599
HpyCH4III ACNGT 2 cut(s) 374, 628
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 1 cut(s) 496
HpyF10VI GCNNNNNNNGC 2 cut(s) 248, 283
HpyF3I CTNAG 3 cut(s) 41, 252, 437
HpySE526I ACGT 1 cut(s) 642
Hsp92II CATG 3 cut(s) 211, 346, 641
HspAI GCGC 4 cut(s) 186, 286, 461, 501
Kzo9I GATC 5 cut(s) 307, 485, 504, 522, 550
LmnI GCTCC 3 cut(s) 274, 478, 634
LweI GCATC 1 cut(s) 273
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 2 cut(s) 535, 622
MalI GATC 5 cut(s) 309, 487, 506, 524, 552
MboI GATC 5 cut(s) 307, 485, 504, 522, 550
MboII GAAGA 2 cut(s) 113, 358
MflI RGATCY 1 cut(s) 550
MhlI GDGCHC 2 cut(s) 419, 483
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 1 cut(s) 16
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 4 cut(s) 450, 543, 552, 597
MmeI TCCRAC 2 cut(s) 120, 177
MnlI CCTC 5 cut(s) 394, 402, 541, 610, 651
Mox20I TGGCCA 1 cut(s) 323
MscI TGGCCA 1 cut(s) 323
MseI TTAA 2 cut(s) 15, 474
MslI CAYNNNNRTG 1 cut(s) 160
Msp20I TGGCCA 1 cut(s) 323
MspCI CTTAAG 1 cut(s) 473
MspI CCGG 1 cut(s) 468
MspR9I CCNGG 1 cut(s) 614
MvaI CCWGG 1 cut(s) 614
MvnI CGCG 1 cut(s) 503
MwoI GCNNNNNNNGC 2 cut(s) 248, 283
NdeII GATC 5 cut(s) 307, 485, 504, 522, 550
NlaIII CATG 3 cut(s) 211, 346, 641
NlaIV GGNNCC 1 cut(s) 552
NmeAIII GCCGAG 1 cut(s) 482
NmuCI GTSAC 2 cut(s) 535, 622
NspV TTCGAA 1 cut(s) 558
PagI TCATGA 1 cut(s) 342
PfeI GAWTC 1 cut(s) 179
PflFI GACNNNGTC 1 cut(s) 533
PflMI CCANNNNNTGG 1 cut(s) 619
PfoI TCCNGGA 1 cut(s) 612
PkrI GCNGC 1 cut(s) 585
PleI GAGTC 4 cut(s) 449, 542, 551, 596
PpsI GAGTC 4 cut(s) 449, 542, 551, 596
Psp6I CCWGG 1 cut(s) 612
PspGI CCWGG 1 cut(s) 612
PspN4I GGNNCC 1 cut(s) 552
PstI CTGCAG 1 cut(s) 498
PsuI RGATCY 1 cut(s) 550
PsyI GACNNNGTC 1 cut(s) 533
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
RseI CAYNNNNRTG 1 cut(s) 160
SaqAI TTAA 2 cut(s) 15, 474
SatI GCNGC 1 cut(s) 584
Sau3AI GATC 5 cut(s) 307, 485, 504, 522, 550
SchI GAGTC 4 cut(s) 450, 543, 552, 597
ScrFI CCNGG 1 cut(s) 614
SduI GDGCHC 2 cut(s) 419, 483
SfaNI GCATC 1 cut(s) 273
SfcI CTRYAG 2 cut(s) 269, 494
SfuI TTCGAA 1 cut(s) 558
SmiMI CAYNNNNRTG 1 cut(s) 160
SmlI CTYRAG 2 cut(s) 189, 473
SmoI CTYRAG 2 cut(s) 189, 473
Sse9I AATT 1 cut(s) 16
SsiI CCGC 3 cut(s) 5, 327, 583
StyD4I CCNGG 1 cut(s) 612
TaaI ACNGT 2 cut(s) 374, 628
TaiI ACGT 1 cut(s) 645
TaqI TCGA 3 cut(s) 405, 546, 558
TasI AATT 1 cut(s) 16
TauI GCSGC 1 cut(s) 586
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 2 cut(s) 15, 474
Tru9I TTAA 2 cut(s) 15, 474
TseFI GTSAC 2 cut(s) 535, 622
Tsp45I GTSAC 2 cut(s) 535, 622
TspDTI ATGAA 2 cut(s) 224, 347
TspGWI ACGGA 3 cut(s) 441, 543, 546
Tth111I GACNNNGTC 1 cut(s) 533
Van91I CCANNNNNTGG 1 cut(s) 619
Vha464I CTTAAG 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.