Rh2BG221000

WPP domain-interacting tail-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000372
Physical Location & Seq
Reverse (-)
21377338 .. 21381077
3740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG221000.1

Sequence Viewer

Length: 426 bp
ATGGACATGGAAACCAGATCTAGGGGAAGATGCTGGTACAATTCATCTCCCGAATCCAATTATCAAGAGTTGGAGAGTTCCATGAAAGTTTTGACAAAAGTGGACTTGGACTTGGCATATTCTGCTGAGAAGTTGGCAAATCTACAGGAGCTTCTGATGCGCCTATTCTCTCAAGAAAATGATCTTGAAGCAATGGCCACCGCAAATAACTATATCATGAGAAACTATTGTGATTCAGATTTTTCTAGATTTTGTTTTAAAAAGTTTGTCATTTCACTATCTATAAGGCCAATGTTGTGGTATATGGTTTTGACTGAAAATCTAGCAGTCTTTTTCCTACTTGTTCTGGTTTTTGTATGTATATTTGTTAAATTAGAGGAGTATAGTTATGATTTTCGCAATCAAGCCTTCTTTGATTTGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008360 GO:0022603 GO:0022604 GO:0032878 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0065007 GO:0065008 GO:2000769
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

16.81

Weight (kDa)

4.88

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WIT1_2_N PF26581 24 - 75 1.4e-15 WIT1/2, N-terminal helical bundle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000372)

Species Orthologous Gene IDs
pyrus_communis pycom04g09000
rosa_chinensis RchiOBHm_Chr1g0319921 RchiOBHm_Chr2g0124671 RchiOBHm_Chr3g0488631 RchiOBHm_Chr3g0495191 RchiOBHm_Chr5g0067451 RchiOBHm_Chr6g0269581 RchiOBHm_Chr7g0210411 RchiOBHm_Chr7g0212341 RchiOBHm_Chr7g0214191
rosa_laevigata RLG00000003882 RLG00000008030 RLG00000011591 RLG00000016235 RLG00000018631 RLG00000031951 RLG00000032135 RLG00000032548 RLG00000033239
rosa_multiflora Rmu_sc0000281.1_g000015 Rmu_sc0000516.1_g000020 Rmu_sc0000816.1_g000025 Rmu_sc0001850.1_g000002 Rmu_sc0003260.1_g000025 Rmu_sc0007581.1_g000007 Rmu_sc0009241.1_g000001
rosa_roxburghii Rroxscaffold_3G00218290 Rroxscaffold_3G00240930 Rroxscaffold_4G00300430 Rroxscaffold_5G00334810 Rroxscaffold_5G00360330 Rroxscaffold_7G00180190 Rroxscaffold_7G00192890
rosa_rugosa Rorug01G0357800 Rorug01G0357900 Rorug03G0350400 Rorug05G0174000 Rorug05G0231100 Rorug05G0231100 Rorug05G0231200 Rorug05G0231200 Rorug05G0577100 Rorug07G0029800 Rorug07G0029900 Rorug07G0146200
rosa_samantha Rh1AG039300 Rh1AG155100 Rh1AG173000 Rh1AG200900 Rh1AG276400 Rh1BG055400 Rh1BG111700 Rh1CG081000 Rh1CG161100 Rh1CG161200 Rh1CG247000 Rh1CG264000 Rh1DG031600 Rh1DG163400 Rh2AG236000 Rh2AG236100 Rh2AG277400 Rh2AG277500 Rh2AG309700 Rh2AG339600 Rh2BG221000 Rh2DG243300 Rh2DG243400 Rh2DG267900 Rh2DG434000 Rh3AG280300 Rh3AG288700 Rh3CG224100 Rh4AG043200 Rh4AG260400 Rh4BG266100 Rh4CG071300 Rh4DG155900 Rh5AG213100 Rh5AG238500 Rh5AG238600 Rh5AG296300 Rh5AG463800 Rh5CG236400 Rh5CG269500 Rh5CG269600 Rh5DG131600 Rh6AG074700 Rh6AG077500 Rh6AG093000 Rh6AG286000 Rh6CG246600 Rh7AG171100 Rh7AG303600 Rh7AG389400 Rh7AG390000 Rh7AG446200 Rh7CG251500 Rh7CG408700 Rh7DG385600
rosa_wichuraiana Rw0G014360 Rw0G015950 Rw2G017860 Rw2G027110 Rw4G037790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 201
AcoI YGGCCR 1 cut(s) 195
AfaI GTAC 1 cut(s) 38
AfiI CCNNNNNNNGG 1 cut(s) 21
AgsI TTSAA 1 cut(s) 188
AluBI AGCT 1 cut(s) 151
AluI AGCT 1 cut(s) 151
AoxI GGCC 2 cut(s) 195, 287
AspLEI GCGC 1 cut(s) 162
BalI TGGCCA 1 cut(s) 197
BfaI CTAG 3 cut(s) 21, 246, 323
BfmI CTRYAG 1 cut(s) 143
BglII AGATCT 1 cut(s) 17
BmsI GCATC 2 cut(s) 20, 147
BpuEI CTTGAG 1 cut(s) 156
Bsc4I CCNNNNNNNGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 198
BseLI CCNNNNNNNGG 1 cut(s) 21
BseMI GCAATG 1 cut(s) 198
BseMII CTCAG 1 cut(s) 117
BseRI GAGGAG 1 cut(s) 392
BshFI GGCC 2 cut(s) 197, 289
BslI CCNNNNNNNGG 1 cut(s) 21
BsnI GGCC 2 cut(s) 197, 289
Bsp143I GATC 2 cut(s) 17, 181
BspACI CCGC 1 cut(s) 201
BspANI GGCC 2 cut(s) 197, 289
BspCNI CTCAG 1 cut(s) 118
BspHI TCATGA 1 cut(s) 216
BsrDI GCAATG 1 cut(s) 198
BssMI GATC 2 cut(s) 17, 181
BstAPI GCANNNNNTGC 1 cut(s) 122
BstDEI CTNAG 1 cut(s) 126
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 2 cut(s) 20, 184
BstMBI GATC 2 cut(s) 17, 181
BstMWI GCNNNNNNNGC 2 cut(s) 122, 157
BstSFI CTRYAG 1 cut(s) 143
BstX2I RGATCY 1 cut(s) 17
BstXI CCANNNNNNTGG 1 cut(s) 297
BstYI RGATCY 1 cut(s) 17
BsuRI GGCC 2 cut(s) 197, 289
CciI TCATGA 1 cut(s) 216
CfoI GCGC 1 cut(s) 162
Csp6I GTAC 1 cut(s) 37
CviAII CATG 3 cut(s) 7, 82, 217
CviJI RGCY 4 cut(s) 151, 197, 289, 407
CviKI_1 RGCY 4 cut(s) 151, 197, 289, 407
CviQI GTAC 1 cut(s) 37
DdeI CTNAG 1 cut(s) 126
DpnI GATC 2 cut(s) 19, 183
DpnII GATC 2 cut(s) 17, 181
DraI TTTAAA 1 cut(s) 259
EaeI YGGCCR 1 cut(s) 195
FaeI CATG 3 cut(s) 10, 85, 220
FatI CATG 3 cut(s) 6, 81, 216
FspBI CTAG 3 cut(s) 21, 246, 323
GlaI GCGC 1 cut(s) 161
HaeIII GGCC 2 cut(s) 197, 289
HhaI GCGC 1 cut(s) 162
Hin1II CATG 3 cut(s) 10, 85, 220
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HinfI GANTC 2 cut(s) 53, 233
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 2 cut(s) 156, 238
Hpy188III TCNNGA 6 cut(s) 50, 65, 173, 185, 217, 246
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 1 cut(s) 418
HpyCH4V TGCA 1 cut(s) 421
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 157
HpyF3I CTNAG 1 cut(s) 126
Hsp92II CATG 3 cut(s) 10, 85, 220
HspAI GCGC 1 cut(s) 160
Kzo9I GATC 2 cut(s) 17, 181
LmnI GCTCC 1 cut(s) 148
LpnPI CCDG 4 cut(s) 19, 28, 131, 332
LweI GCATC 2 cut(s) 20, 147
MaeI CTAG 3 cut(s) 21, 246, 323
MalI GATC 2 cut(s) 19, 183
MboI GATC 2 cut(s) 17, 181
MboII GAAGA 1 cut(s) 39
MflI RGATCY 1 cut(s) 17
MlsI TGGCCA 1 cut(s) 197
MluCI AATT 3 cut(s) 40, 58, 371
MluNI TGGCCA 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 1 cut(s) 370
Mox20I TGGCCA 1 cut(s) 197
MscI TGGCCA 1 cut(s) 197
MseI TTAA 3 cut(s) 258, 369, 424
Msp20I TGGCCA 1 cut(s) 197
MwoI GCNNNNNNNGC 2 cut(s) 122, 157
NdeII GATC 2 cut(s) 17, 181
NlaIII CATG 3 cut(s) 10, 85, 220
PagI TCATGA 1 cut(s) 216
PfeI GAWTC 2 cut(s) 53, 233
PsuI RGATCY 1 cut(s) 17
RsaI GTAC 1 cut(s) 38
RsaNI GTAC 1 cut(s) 37
SaqAI TTAA 3 cut(s) 258, 369, 424
Sau3AI GATC 2 cut(s) 17, 181
SetI ASST 1 cut(s) 153
SfaNI GCATC 2 cut(s) 20, 147
SfcI CTRYAG 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 171
SmoI CTYRAG 1 cut(s) 171
Sse9I AATT 3 cut(s) 40, 58, 371
SsiI CCGC 1 cut(s) 201
SspMI CTAG 3 cut(s) 21, 246, 323
TasI AATT 3 cut(s) 40, 58, 371
TfiI GAWTC 2 cut(s) 53, 233
Tru1I TTAA 3 cut(s) 258, 369, 424
Tru9I TTAA 3 cut(s) 258, 369, 424
TspDTI ATGAA 2 cut(s) 33, 98
XbaI TCTAGA 1 cut(s) 245
XspI CTAG 3 cut(s) 21, 246, 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.