Rroxscaffold_7G00192890

WPP domain-interacting tail-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000372
Physical Location & Seq
Reverse (-)
34250446 .. 34260376
9931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00192890.1

Sequence Viewer

Length: 885 bp
ATGTCGGTTAGTGAATCCGGCGCTCAAGAGTTGGAGAGTTCCATGAAAGTTTTGACAAAGGTGGACTTGGACTTGGCATATTCTGCCGAGAAGTTGGCAAATCTACGGGAGCTTAAGATGCGCCTATTCTCTCAAGAAAATGATCTTGAAGCAATGGCCACCGCAAATAACTATATCATGAGAAACTATTCAAGTGGCATACTTTTGTTGCTTCGCTTATTGCTCGAGCCTCCCTCCTGCAAAATTGATCAACATGTTAAAAGAAAATCAAGCTCACATCCTTATGGGAGCCCTAGTGGTTATTCACTTATTAATGGGTGCATAGCCGTAGTAGGATCCAGGAAGAGGCCGGGGGACGGGGAGCGCGCTCTCTGTCGGGCTTCTTGTCTCTGTGCCCATTGTTTTTGCTTTGCGGCTTGTTTTTCCCATTCGGTGCGGTTTTGGGTGGAGGCTTGGCCTAGGGATGGTAGGCGGTGGATCTCGGACGGTGAAGATCGATCTGTTTTCGTTCTTCTCGTTTCGGGGTCAAGGTGTCTCGGCGTGGTGCAATTTGCCGGCGCTTCGGTGTCGAGGCGAGAGGATGGAGGATTGGCATTGCTTGGCGATGGCGGGCCGAGGTTGGAGGCTTGGCGAAGGCCGGGTTCTCAATCTCATAGCGGAGGCGAGCCCGGAAGAGATCCGATTTCTCCGGGGTTTCGTTTCGTTGTCATCCGAGCACCGGGATGGCCCGCTTGCGGTACGACGGCGTTGTCGTGGTGGGTTTTTTGCGGCGGCGCGGCGGCGTGCGAGAGGGCGGGATGGAGGAGCGCGCGGGCACTCGGCCTTGGTGGATTGCGGCATCGGATCCTTGTGGGGACCGGTTTTTGGGCCGGCCTTTGTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008360 GO:0022603 GO:0022604 GO:0032878 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0065007 GO:0065008 GO:2000769
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

294

Amino Acids

31.85

Weight (kDa)

9.13

Isoelectric Point (pI)

52.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WIT1_2_N PF26581 11 - 61 8.1e-14 WIT1/2, N-terminal helical bundle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000372)

Species Orthologous Gene IDs
pyrus_communis pycom04g09000
rosa_chinensis RchiOBHm_Chr1g0319921 RchiOBHm_Chr2g0124671 RchiOBHm_Chr3g0488631 RchiOBHm_Chr3g0495191 RchiOBHm_Chr5g0067451 RchiOBHm_Chr6g0269581 RchiOBHm_Chr7g0210411 RchiOBHm_Chr7g0212341 RchiOBHm_Chr7g0214191
rosa_laevigata RLG00000003882 RLG00000008030 RLG00000011591 RLG00000016235 RLG00000018631 RLG00000031951 RLG00000032135 RLG00000032548 RLG00000033239
rosa_multiflora Rmu_sc0000281.1_g000015 Rmu_sc0000516.1_g000020 Rmu_sc0000816.1_g000025 Rmu_sc0001850.1_g000002 Rmu_sc0003260.1_g000025 Rmu_sc0007581.1_g000007 Rmu_sc0009241.1_g000001
rosa_roxburghii Rroxscaffold_3G00218290 Rroxscaffold_3G00240930 Rroxscaffold_4G00300430 Rroxscaffold_5G00334810 Rroxscaffold_5G00360330 Rroxscaffold_7G00180190 Rroxscaffold_7G00192890
rosa_rugosa Rorug01G0357800 Rorug01G0357900 Rorug03G0350400 Rorug05G0174000 Rorug05G0231100 Rorug05G0231100 Rorug05G0231200 Rorug05G0231200 Rorug05G0577100 Rorug07G0029800 Rorug07G0029900 Rorug07G0146200
rosa_samantha Rh1AG039300 Rh1AG155100 Rh1AG173000 Rh1AG200900 Rh1AG276400 Rh1BG055400 Rh1BG111700 Rh1CG081000 Rh1CG161100 Rh1CG161200 Rh1CG247000 Rh1CG264000 Rh1DG031600 Rh1DG163400 Rh2AG236000 Rh2AG236100 Rh2AG277400 Rh2AG277500 Rh2AG309700 Rh2AG339600 Rh2BG221000 Rh2DG243300 Rh2DG243400 Rh2DG267900 Rh2DG434000 Rh3AG280300 Rh3AG288700 Rh3CG224100 Rh4AG043200 Rh4AG260400 Rh4BG266100 Rh4CG071300 Rh4DG155900 Rh5AG213100 Rh5AG238500 Rh5AG238600 Rh5AG296300 Rh5AG463800 Rh5CG236400 Rh5CG269500 Rh5CG269600 Rh5DG131600 Rh6AG074700 Rh6AG077500 Rh6AG093000 Rh6AG286000 Rh6CG246600 Rh7AG171100 Rh7AG303600 Rh7AG389400 Rh7AG390000 Rh7AG446200 Rh7CG251500 Rh7CG408700 Rh7DG385600
rosa_wichuraiana Rw0G014360 Rw0G015950 Rw2G017860 Rw2G027110 Rw4G037790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 748
AccII CGCG 4 cut(s) 366, 776, 809, 811
AclWI GGATC 6 cut(s) 330, 343, 485, 671, 838, 851
AcoI YGGCCR 1 cut(s) 156
AfaI GTAC 1 cut(s) 739
AfiI CCNNNNNNNGG 6 cut(s) 345, 356, 464, 718, 734, 864
AflII CTTAAG 1 cut(s) 113
AflIII ACRYGT 1 cut(s) 253
AgeI ACCGGT 1 cut(s) 857
AgsI TTSAA 2 cut(s) 149, 192
AjnI CCWGG 1 cut(s) 338
AluBI AGCT 2 cut(s) 112, 273
AluI AGCT 2 cut(s) 112, 273
Alw21I GWGCWC 1 cut(s) 718
Alw26I GTCTC 2 cut(s) 392, 539
AlwI GGATC 6 cut(s) 330, 343, 485, 671, 838, 851
Ama87I CYCGRG 1 cut(s) 224
AoxI GGCC 9 cut(s) 156, 347, 455, 611, 635, 725, 820, 867, 871
AseI ATTAAT 1 cut(s) 312
AsiGI ACCGGT 1 cut(s) 857
Asp700I GAANNNNTTC 1 cut(s) 187
AspA2I CCTAGG 1 cut(s) 458
AspLEI GCGC 8 cut(s) 23, 123, 366, 368, 560, 776, 809, 811
AspS9I GGNCC 4 cut(s) 611, 726, 855, 867
AsuC2I CCSGG 5 cut(s) 351, 639, 669, 690, 720
AsuHPI GGTGA 1 cut(s) 500
AvaI CYCGRG 1 cut(s) 224
AvaII GGWCC 1 cut(s) 855
AvrII CCTAGG 1 cut(s) 458
BaeGI GKGCMC 2 cut(s) 397, 817
BalI TGGCCA 1 cut(s) 158
BamHI GGATCC 2 cut(s) 335, 843
BanII GRGCYC 2 cut(s) 293, 669
Bbv12I GWGCWC 1 cut(s) 718
BccI CCATC 5 cut(s) 458, 575, 599, 717, 792
BceAI ACGGC 2 cut(s) 311, 759
BciT130I CCWGG 1 cut(s) 340
BclI TGATCA 1 cut(s) 247
BcnI CCSGG 5 cut(s) 351, 639, 669, 690, 720
BcoDI GTCTC 2 cut(s) 392, 539
BfaI CTAG 2 cut(s) 294, 459
BfoI RGCGCY 2 cut(s) 24, 561
BfrI CTTAAG 1 cut(s) 113
BisI GCNGC 6 cut(s) 414, 769, 772, 777, 780, 836
BlnI CCTAGG 1 cut(s) 458
BlsI GCNGC 6 cut(s) 415, 770, 773, 778, 781, 837
Bme1390I CCNGG 6 cut(s) 340, 351, 639, 669, 690, 720
Bme18I GGWCC 1 cut(s) 855
BmeT110I CYCGRG 1 cut(s) 224
BmgT120I GGNCC 4 cut(s) 611, 726, 855, 867
BmiI GGNNCC 4 cut(s) 290, 337, 845, 856
BmrFI CCNGG 6 cut(s) 340, 351, 639, 669, 690, 720
BmsI GCATC 2 cut(s) 108, 847
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpuEI CTTGAG 2 cut(s) 9, 117
BpuMI CCSGG 5 cut(s) 351, 639, 669, 690, 720
Bsa29I ATCGAT 1 cut(s) 496
BsaJI CCNNGG 5 cut(s) 350, 458, 614, 689, 823
BsaWI WCCGGW 1 cut(s) 857
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bsc4I CCNNNNNNNGG 6 cut(s) 345, 356, 464, 718, 734, 864
Bse118I RCCGGY 3 cut(s) 554, 857, 869
Bse3DI GCAATG 2 cut(s) 159, 593
BseBI CCWGG 1 cut(s) 340
BseCI ATCGAT 1 cut(s) 496
BseDI CCNNGG 5 cut(s) 350, 458, 614, 689, 823
BseGI GGATG 6 cut(s) 277, 469, 586, 708, 728, 803
BseLI CCNNNNNNNGG 6 cut(s) 345, 356, 464, 718, 734, 864
BseMI GCAATG 2 cut(s) 159, 593
BsePI GCGCGC 2 cut(s) 364, 807
BseRI GAGGAG 1 cut(s) 817
BseSI GKGCMC 2 cut(s) 397, 817
Bsh1236I CGCG 4 cut(s) 366, 776, 809, 811
BshFI GGCC 9 cut(s) 158, 349, 457, 613, 637, 727, 822, 869, 873
BshTI ACCGGT 1 cut(s) 857
BshVI ATCGAT 1 cut(s) 496
BsiHKAI GWGCWC 1 cut(s) 718
BsiHKCI CYCGRG 1 cut(s) 224
BsiSI CCGG 9 cut(s) 18, 350, 555, 638, 669, 689, 719, 858, 870
BslFI GGGAC 2 cut(s) 368, 868
BslI CCNNNNNNNGG 6 cut(s) 345, 356, 464, 718, 734, 864
BsmAI GTCTC 2 cut(s) 392, 539
BsmFI GGGAC 2 cut(s) 368, 868
BsnI GGCC 9 cut(s) 158, 349, 457, 613, 637, 727, 822, 869, 873
BsoBI CYCGRG 1 cut(s) 224
Bsp1286I GDGCHC 5 cut(s) 293, 397, 669, 718, 817
Bsp143I GATC 8 cut(s) 142, 247, 335, 477, 493, 497, 676, 843
BspANI GGCC 9 cut(s) 158, 349, 457, 613, 637, 727, 822, 869, 873
BspDI ATCGAT 1 cut(s) 496
BspFNI CGCG 4 cut(s) 366, 776, 809, 811
BspHI TCATGA 1 cut(s) 177
BspLI GGNNCC 4 cut(s) 290, 337, 845, 856
BspPI GGATC 6 cut(s) 330, 343, 485, 671, 838, 851
BspTI CTTAAG 1 cut(s) 113
BsrDI GCAATG 2 cut(s) 159, 593
BsrFI RCCGGY 3 cut(s) 554, 857, 869
BssAI RCCGGY 3 cut(s) 554, 857, 869
BssECI CCNNGG 5 cut(s) 350, 458, 614, 689, 823
BssHII GCGCGC 2 cut(s) 364, 807
BssMI GATC 8 cut(s) 142, 247, 335, 477, 493, 497, 676, 843
BssT1I CCWWGG 2 cut(s) 458, 823
Bst2UI CCWGG 1 cut(s) 340
Bst4CI ACNGT 1 cut(s) 488
Bst6I CTCTTC 2 cut(s) 338, 667
BstAFI CTTAAG 1 cut(s) 113
BstAPI GCANNNNNTGC 1 cut(s) 83
BstDEI CTNAG 1 cut(s) 882
BstF5I GGATG 6 cut(s) 277, 469, 586, 708, 728, 803
BstFNI CGCG 4 cut(s) 366, 776, 809, 811
BstH2I RGCGCY 2 cut(s) 24, 561
BstHHI GCGC 8 cut(s) 23, 123, 366, 368, 560, 776, 809, 811
BstKTI GATC 8 cut(s) 145, 250, 338, 480, 496, 500, 679, 846
BstMAI GTCTC 2 cut(s) 392, 539
BstMBI GATC 8 cut(s) 142, 247, 335, 477, 493, 497, 676, 843
BstMWI GCNNNNNNNGC 2 cut(s) 83, 118
BstNI CCWGG 1 cut(s) 340
BstNSI RCATGY 1 cut(s) 257
BstSCI CCNGG 6 cut(s) 338, 349, 637, 667, 688, 718
BstSLI GKGCMC 2 cut(s) 397, 817
BstUI CGCG 4 cut(s) 366, 776, 809, 811
BstX2I RGATCY 4 cut(s) 335, 477, 676, 843
BstYI RGATCY 4 cut(s) 335, 477, 676, 843
Bsu15I ATCGAT 1 cut(s) 496
BsuRI GGCC 9 cut(s) 158, 349, 457, 613, 637, 727, 822, 869, 873
BsuTUI ATCGAT 1 cut(s) 496
BtgZI GCGATG 1 cut(s) 618
BtsCI GGATG 6 cut(s) 277, 469, 586, 708, 728, 803
CciI TCATGA 1 cut(s) 177
CfoI GCGC 8 cut(s) 23, 123, 366, 368, 560, 776, 809, 811
Cfr10I RCCGGY 3 cut(s) 554, 857, 869
Cfr13I GGNCC 4 cut(s) 611, 726, 855, 867
ClaI ATCGAT 1 cut(s) 496
Csp6I GTAC 1 cut(s) 738
CspAI ACCGGT 1 cut(s) 857
CviAII CATG 3 cut(s) 43, 178, 254
CviQI GTAC 1 cut(s) 738
DdeI CTNAG 1 cut(s) 882
DpnI GATC 8 cut(s) 144, 249, 337, 479, 495, 499, 678, 845
DpnII GATC 8 cut(s) 142, 247, 335, 477, 493, 497, 676, 843
DrdI GACNNNNNNGTC 1 cut(s) 748
DseDI GACNNNNNNGTC 1 cut(s) 748
EaeI YGGCCR 1 cut(s) 156
Eam1104I CTCTTC 2 cut(s) 338, 667
EarI CTCTTC 2 cut(s) 338, 667
Eco130I CCWWGG 2 cut(s) 458, 823
Eco24I GRGCYC 2 cut(s) 293, 669
Eco47I GGWCC 1 cut(s) 855
Eco88I CYCGRG 1 cut(s) 224
EcoRII CCWGG 1 cut(s) 338
EcoT14I CCWWGG 2 cut(s) 458, 823
EcoT38I GRGCYC 2 cut(s) 293, 669
ErhI CCWWGG 2 cut(s) 458, 823
FaeI CATG 3 cut(s) 46, 181, 257
FaiI YATR 9 cut(s) 44, 79, 174, 179, 200, 255, 285, 323, 654
FalI AAGNNNNNCTT 2 cut(s) 50, 82
FaqI GGGAC 2 cut(s) 368, 868
FatI CATG 3 cut(s) 42, 177, 253
FauI CCCGC 4 cut(s) 602, 736, 787, 804
FbaI TGATCA 1 cut(s) 247
Fnu4HI GCNGC 6 cut(s) 414, 769, 772, 777, 780, 836
FokI GGATG 6 cut(s) 264, 476, 593, 695, 735, 810
FriOI GRGCYC 2 cut(s) 293, 669
FseI GGCCGGCC 1 cut(s) 873
Fsp4HI GCNGC 6 cut(s) 414, 769, 772, 777, 780, 836
FspBI CTAG 2 cut(s) 294, 459
GlaI GCGC 8 cut(s) 22, 122, 365, 367, 559, 775, 808, 810
GluI GCNGC 6 cut(s) 414, 769, 772, 777, 780, 836
HaeII RGCGCY 2 cut(s) 24, 561
HaeIII GGCC 9 cut(s) 158, 349, 457, 613, 637, 727, 822, 869, 873
HapII CCGG 9 cut(s) 18, 350, 555, 638, 669, 689, 719, 858, 870
HhaI GCGC 8 cut(s) 23, 123, 366, 368, 560, 776, 809, 811
Hin1II CATG 3 cut(s) 46, 181, 257
Hin6I GCGC 8 cut(s) 21, 121, 364, 366, 558, 774, 807, 809
HinP1I GCGC 8 cut(s) 21, 121, 364, 366, 558, 774, 807, 809
HinfI GANTC 1 cut(s) 14
HpaII CCGG 9 cut(s) 18, 350, 555, 638, 669, 689, 719, 858, 870
HphI GGTGA 1 cut(s) 500
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 4 cut(s) 484, 681, 713, 843
Hpy188III TCNNGA 4 cut(s) 26, 134, 146, 178
Hpy8I GTNNAC 1 cut(s) 64
Hpy99I CGWCG 1 cut(s) 745
HpyAV CCTTC 1 cut(s) 627
HpyCH4III ACNGT 1 cut(s) 488
HpyCH4V TGCA 3 cut(s) 240, 321, 547
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 118
HpyF3I CTNAG 1 cut(s) 882
Hsp92II CATG 3 cut(s) 46, 181, 257
HspAI GCGC 8 cut(s) 21, 121, 364, 366, 558, 774, 807, 809
KroI GCCGGC 2 cut(s) 554, 869
KroNI GCCGGC 2 cut(s) 556, 871
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 8 cut(s) 142, 247, 335, 477, 493, 497, 676, 843
LmnI GCTCC 4 cut(s) 109, 288, 361, 804
LweI GCATC 2 cut(s) 108, 847
MaeI CTAG 2 cut(s) 294, 459
MalI GATC 8 cut(s) 144, 249, 337, 479, 495, 499, 678, 845
MboI GATC 8 cut(s) 142, 247, 335, 477, 493, 497, 676, 843
MboII GAAGA 4 cut(s) 355, 503, 503, 684
MflI RGATCY 4 cut(s) 335, 477, 676, 843
MhlI GDGCHC 5 cut(s) 293, 397, 669, 718, 817
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 2 cut(s) 243, 548
MluNI TGGCCA 1 cut(s) 158
MmeI TCCRAC 2 cut(s) 12, 600
Mox20I TGGCCA 1 cut(s) 158
MroNI GCCGGC 2 cut(s) 554, 869
MroXI GAANNNNTTC 1 cut(s) 187
MscI TGGCCA 1 cut(s) 158
MseI TTAA 3 cut(s) 114, 258, 312
MslI CAYNNNNRTG 2 cut(s) 282, 721
Msp20I TGGCCA 1 cut(s) 158
MspCI CTTAAG 1 cut(s) 113
MspI CCGG 9 cut(s) 18, 350, 555, 638, 669, 689, 719, 858, 870
MspR9I CCNGG 6 cut(s) 340, 351, 639, 669, 690, 720
MvaI CCWGG 1 cut(s) 340
MvnI CGCG 4 cut(s) 366, 776, 809, 811
MwoI GCNNNNNNNGC 2 cut(s) 83, 118
NaeI GCCGGC 2 cut(s) 556, 871
NciI CCSGG 5 cut(s) 351, 639, 669, 690, 720
NdeII GATC 8 cut(s) 142, 247, 335, 477, 493, 497, 676, 843
NgoMIV GCCGGC 2 cut(s) 554, 869
NlaIII CATG 3 cut(s) 46, 181, 257
NlaIV GGNNCC 4 cut(s) 290, 337, 845, 856
NmeAIII GCCGAG 4 cut(s) 112, 516, 639, 798
NspI RCATGY 1 cut(s) 257
PaeR7I CTCGAG 1 cut(s) 224
PagI TCATGA 1 cut(s) 177
PauI GCGCGC 2 cut(s) 364, 807
PciI ACATGT 1 cut(s) 253
PcsI WCGNNNNNNNCGW 2 cut(s) 513, 749
PdiI GCCGGC 2 cut(s) 556, 871
PdmI GAANNNNTTC 1 cut(s) 187
PfeI GAWTC 1 cut(s) 14
PfoI TCCNGGA 1 cut(s) 338
PinAI ACCGGT 1 cut(s) 857
PkrI GCNGC 6 cut(s) 415, 770, 773, 778, 781, 837
PscI ACATGT 1 cut(s) 253
PshBI ATTAAT 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 338
PspGI CCWGG 1 cut(s) 338
PspN4I GGNNCC 4 cut(s) 290, 337, 845, 856
PspPI GGNCC 4 cut(s) 611, 726, 855, 867
PspXI VCTCGAGB 1 cut(s) 224
PsuI RGATCY 4 cut(s) 335, 477, 676, 843
PteI GCGCGC 2 cut(s) 364, 807
RigI GGCCGGCC 1 cut(s) 873
RsaI GTAC 1 cut(s) 739
RsaNI GTAC 1 cut(s) 738
RseI CAYNNNNRTG 2 cut(s) 282, 721
SaqAI TTAA 3 cut(s) 114, 258, 312
SatI GCNGC 6 cut(s) 414, 769, 772, 777, 780, 836
Sau3AI GATC 8 cut(s) 142, 247, 335, 477, 493, 497, 676, 843
Sau96I GGNCC 4 cut(s) 611, 726, 855, 867
ScrFI CCNGG 6 cut(s) 340, 351, 639, 669, 690, 720
SduI GDGCHC 5 cut(s) 293, 397, 669, 718, 817
SetI ASST 5 cut(s) 63, 114, 275, 533, 620
SfaNI GCATC 2 cut(s) 108, 847
Sfr274I CTCGAG 1 cut(s) 224
SinI GGWCC 1 cut(s) 855
SlaI CTCGAG 1 cut(s) 224
SmiMI CAYNNNNRTG 2 cut(s) 282, 721
SmlI CTYRAG 4 cut(s) 24, 113, 132, 224
SmoI CTYRAG 4 cut(s) 24, 113, 132, 224
Sse9I AATT 2 cut(s) 243, 548
SspMI CTAG 2 cut(s) 294, 459
StyD4I CCNGG 6 cut(s) 338, 349, 637, 667, 688, 718
StyI CCWWGG 2 cut(s) 458, 823
TaaI ACNGT 1 cut(s) 488
TaqI TCGA 3 cut(s) 225, 496, 569
TasI AATT 2 cut(s) 243, 548
TauI GCSGC 6 cut(s) 416, 771, 774, 779, 782, 838
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 3 cut(s) 114, 258, 312
Tru9I TTAA 3 cut(s) 114, 258, 312
TspDTI ATGAA 1 cut(s) 59
Vha464I CTTAAG 1 cut(s) 113
VpaK11BI GGWCC 1 cut(s) 855
VspI ATTAAT 1 cut(s) 312
XceI RCATGY 1 cut(s) 257
XhoI CTCGAG 1 cut(s) 224
XmaJI CCTAGG 1 cut(s) 458
XmnI GAANNNNTTC 1 cut(s) 187
XspI CTAG 2 cut(s) 294, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.