Rmu_sc0001648.1_g000026

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001648.1
OG0000779
Physical Location & Seq
Forward (+)
184517 .. 188002
3486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001648.1_g000026.1.cds

Sequence Viewer

Length: 2985 bp
atgtggtatcagagcccatcgattgggctgatggggcaagggtcaccggagctgtcggttgctacgaggcaatatcaggtttcacctagttttgcaccgacgaggacggcggtgttggacgacagttttgctcaacctactggtacatacactggtttctctctctattcgcattcatatacaccacatattccaccaccgcaatactacccaccatcacaatcgtttacccaaaatcctcaatttccatcagcccatactcaccattcacttgattctcccccaaatcccgggatctatccttcgatttcacaaagccattcctatgaaccatcagttaccggtgttaattcacacttcgtctccaccaaaacagtgcctacaccacctaattatcattctgctccttcacctctcgtttcggctactctcatacctgagcctacggtcaagcttaaggaggctaagaaaccacattcaccaccacaaggttctgaaaaatcgcaagaatcggtgaaaagttcgatggataccttcatatctgaattcagggaggagctacgattgttaaggggttcattgcaatctccggcaagccaagttgataacccactaaagtttggctctccgccgccgtcgtcgacacccaaattgattccggctgcaactgaggcttcttcatctctaattcctgctgataatggtattattcataaaacccatatggatagtggcttgaatttatctcacactgatgtgatattaggatttaatgataatactagtacggatcatgttgtgggttttgtgtttggtattgatgtgaatttagaaactggggagggagagagttctaataagcgggttgaggtgaatagtggatatggtagagagttcaatgagcttgagagtgtcagtgctaagcaggatgatttgggattgagtttgtgtggggagaggagagaatgtagcgctgataaaagtggaaaagggatattggagaatgaaattgagtatgatcagaaccgttcgactccaaatttgaatattggctttagcaacaccaacacctttaatcaaccagagccaggcaacacaagtggcctcgttggtttagcatgcatttctggtcagagcaactttggacaatgttctgtagctcaaggctttgcagctgtacaaccgatacatgctacaagcccaattggaacgcttactgaatccgttgaattagtgagctcgctgctgtcaattcagacttcttcagcaccagttcagctgtggtctccaactagaagtcttgtatcagaggagagagatgtcgtcaaaaagaagactggtgaacttacagagcttgataatctatctactaaggtaggtaattacttccagtatgccttgcctaagtgttgtgggtttggaaagcatattagtgcatgttctgtggtgaatgtttttaatgcaatgtgtgtaatgcttaagcattacattgagagtccaaagaagattaatcactatgttctgcaactatgcttctctgggtttgataagaaggtacgtgataaaggtagtggccacactagcttaatagatgaattttctatcctgaatagaaagtttgcagagaatatgaggctacttggcgttgattttcgtgtaatgaaagtttttgttgtagaaaatgccaatgatcttaatgcagctgtaaatgatgtgctgaatgagagtaaagaattgattcaccagcaggtggataaggaagtagaggttgagctatttcaaacagcaggattctctgatgttgtatctaaaatcagtgctcatgaggaagaaggaatctattctccatggaatgaggaatattttctagaggaaattgttagtacaagacctaatccaagaaatattgtgcagcaatatgaatattcaaatattggagctaccgaattgattttatcaaatggcatgagagttagctataagagtacagactttgttgatgatcagtttatctttacaagtttctcatatgggggtttggccatacttgttgaaatagagtacttttcttgcatgctggggccaactattgcaggagaaattggtgtatatggttatagaccttcagctttggtcgataagcttgcgtgtaagagggccgaagtcagcacaaagtttggagcatacatgagaatctctaattacaatgccaacaatactgatcaaacaaatttcacaagttggttgtatgttgatctgaggtattgttcttcttcggctatctcaattgttgctactcttcttctttctgtggcattagttatgtgtgttactatttttggtgtgatgaagactatagaaaaagcacacattgatggaattgttgagaaagctaagaagaggcaacttgatgttattttgggattaggtgaggataagtcattttttacaatattgctgcaacatggaattcctatggaaccgacagaatctttgcatacgcagatgactgctattagttttgtctgttcctacatggtaattaaaattgctaaatttcgagagacaaggagttttcaaaaacaacgtgttgtcatcatatggtctagagctctttcaagtttcaaaagatatgtggtactgacatcagccatcttgcatagaagatttgcaatgaatagagcagctgcaacaacatttttcatgaaacacttgtactgtaattggtttcacttctatgctggagtgactgctgatatacatttaaaccatcactgggaagagggctttcctaatgatccaactgggagatttaatcagttcaaagcagttgtaagagatgtccagattttgactcaaaatgtgaaacttgaggacaagtttctgtttaagggggaagcaatgttaggaccccaatttcctattaggctttactatgactacccagcccatccttatggcccattactgatttatgcaagctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH Peptidase_M16 Peptidase_M16_C

Protein Analysis

994

Amino Acids

110.28

Weight (kDa)

6.08

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000779)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g29862
rosa_chinensis RchiOBHm_Chr1g0313641 RchiOBHm_Chr4g0408161 RchiOBHm_Chr4g0408171 RchiOBHm_Chr4g0437611 RchiOBHm_Chr4g0437621 RchiOBHm_Chr4g0444671 RchiOBHm_Chr5g0045021 RchiOBHm_Chr5g0070121 RchiOBHm_Chr5g0082551 RchiOBHm_Chr6g0247501 RchiOBHm_Chr6g0271311 RchiOBHm_Chr6g0290811 RchiOBHm_Chr6g0310571
rosa_laevigata RLG00000004718 RLG00000008552 RLG00000015595
rosa_multiflora Rmu_co8342433.1_g000001 Rmu_sc0000147.1_g000080 Rmu_sc0000391.1_g000018 Rmu_sc0000753.1_g000021 Rmu_sc0000870.1_g000071 Rmu_sc0000870.1_g000072 Rmu_sc0001648.1_g000026 Rmu_sc0001648.1_g000043 Rmu_sc0001972.1_g000009 Rmu_sc0002109.1_g000009 Rmu_sc0003340.1_g000008 Rmu_sc0003340.1_g000009 Rmu_sc0004874.1_g000014 Rmu_sc0005823.1_g000009 Rmu_sc0005941.1_g000006 Rmu_sc0009850.1_g000015 Rmu_sc0015511.1_g000012
rosa_roxburghii Rroxscaffold_1G00034320 Rroxscaffold_1G00036130 Rroxscaffold_1G00043440 Rroxscaffold_1G00043450 Rroxscaffold_1G00050660 Rroxscaffold_1G00050670 Rroxscaffold_1G00066940 Rroxscaffold_2G00094100 Rroxscaffold_2G00120240 Rroxscaffold_4G00287900 Rroxscaffold_4G00287910 Rroxscaffold_4G00290700 Rroxscaffold_4G00290710 Rroxscaffold_4G00298640 Rroxscaffold_4G00308540 Rroxscaffold_5G00369420 Rroxscaffold_7G00217900
rosa_rugosa Rorug06G0077100
rosa_samantha Rh1AG005600 Rh1AG005700 Rh1BG433000 Rh1DG003900 Rh2CG286600 Rh3AG243900 Rh3BG368800 Rh5AG301800 Rh5BG309500 Rh5CG501500 Rh5CG501600 Rh5DG320200 Rh6AG179500 Rh6CG338800 Rh6CG405000 Rh6DG092100 Rh7AG294100 Rh7CG239600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1738
Acc36I ACCTGC 1 cut(s) 1738
AccB7I CCANNNNNTGG 2 cut(s) 23, 1750
AccI GTMKAC 1 cut(s) 641
AciI CCGC 5 cut(s) 110, 200, 629, 632, 862
AclWI GGATC 3 cut(s) 302, 798, 2792
AcoI YGGCCR 2 cut(s) 1573, 2040
AcsI RAATTY 8 cut(s) 545, 739, 826, 1039, 1595, 2230, 2469, 2555
AcuI CTGAAG 2 cut(s) 1248, 2109
AfaI GTAC 9 cut(s) 145, 787, 1179, 1557, 1885, 1987, 2063, 2640, 2717
AfeI AGCGCT 1 cut(s) 973
AfiI CCNNNNNNNGG 6 cut(s) 23, 290, 488, 1088, 1750, 2776
AflII CTTAAG 2 cut(s) 455, 1478
AflIII ACRYGT 1 cut(s) 2587
AgeI ACCGGT 1 cut(s) 341
AhlI ACTAGT 1 cut(s) 782
AjnI CCWGG 1 cut(s) 1087
AleI CACNNNNGTG 1 cut(s) 755
Alw21I GWGCWC 3 cut(s) 1241, 1822, 2614
Alw26I GTCTC 3 cut(s) 367, 1290, 2558
AlwI GGATC 3 cut(s) 302, 798, 2792
Ama87I CYCGRG 1 cut(s) 290
Aor51HI AGCGCT 1 cut(s) 973
AoxI GGCC 6 cut(s) 1102, 1573, 2040, 2081, 2157, 2959
ApeKI GCWGC 8 cut(s) 662, 1172, 1243, 1700, 1912, 2458, 2684, 2687
ApoI RAATTY 8 cut(s) 545, 739, 826, 1039, 1595, 2230, 2469, 2555
ArsI GACNNNNNNTTYG 6 cut(s) 110, 142, 1024, 1056, 2476, 2508
AseI ATTAAT 1 cut(s) 1509
AsiGI ACCGGT 1 cut(s) 341
Asp700I GAANNNNTTC 3 cut(s) 1737, 1840, 1863
AspLEI GCGC 1 cut(s) 974
AspS9I GGNCC 4 cut(s) 2081, 2157, 2909, 2960
AsuC2I CCSGG 2 cut(s) 291, 292
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 1 cut(s) 2909
BalI TGGCCA 2 cut(s) 1575, 2042
BanII GRGCYC 3 cut(s) 17, 1241, 2614
BbsI GAAGAC 2 cut(s) 1340, 2357
Bbv12I GWGCWC 3 cut(s) 1241, 1822, 2614
BbvI GCAGC 8 cut(s) 649, 1184, 1230, 1712, 1924, 2445, 2674, 2696
BceAI ACGGC 2 cut(s) 123, 619
BcgI CGANNNNNNTGC 4 cut(s) 110, 144, 2126, 2160
BciT130I CCWGG 1 cut(s) 1089
BciVI GTATCC 1 cut(s) 523
BclI TGATCA 3 cut(s) 1018, 2002, 2221
BcnI CCSGG 2 cut(s) 291, 292
BcoDI GTCTC 3 cut(s) 367, 1290, 2558
BcuI ACTAGT 1 cut(s) 782
BfaI CTAG 6 cut(s) 87, 783, 1293, 1581, 1868, 2607
BfmI CTRYAG 2 cut(s) 1155, 2355
BfoI RGCGCY 1 cut(s) 975
BfrI CTTAAG 2 cut(s) 455, 1478
BfuAI ACCTGC 1 cut(s) 1738
BfuI GTATCC 1 cut(s) 523
BisI GCNGC 9 cut(s) 632, 663, 1173, 1244, 1701, 1913, 2459, 2685, 2688
BlpI GCTNAGC 1 cut(s) 921
BlsI GCNGC 9 cut(s) 633, 664, 1174, 1245, 1702, 1914, 2460, 2686, 2689
BmcAI AGTACT 1 cut(s) 2063
Bme1390I CCNGG 3 cut(s) 291, 292, 1089
Bme18I GGWCC 1 cut(s) 2909
BmeT110I CYCGRG 1 cut(s) 290
BmgT120I GGNCC 4 cut(s) 2081, 2157, 2909, 2960
BmiI GGNNCC 3 cut(s) 2082, 2481, 2911
BmrFI CCNGG 3 cut(s) 291, 292, 1089
BmrI ACTGGG 3 cut(s) 846, 2785, 2814
BmuI ACTGGG 3 cut(s) 846, 2785, 2814
BpiI GAAGAC 2 cut(s) 1340, 2357
BpmI CTGGAG 1 cut(s) 2763
Bpu10I CCTNAGC 1 cut(s) 438
Bpu1102I GCTNAGC 1 cut(s) 921
BpuEI CTTGAG 3 cut(s) 926, 1146, 2891
BpuMI CCSGG 2 cut(s) 291, 292
Bsa29I ATCGAT 1 cut(s) 20
BsaAI YACGTR 1 cut(s) 1559
BsaI GGTCTC 1 cut(s) 1290
BsaJI CCNNGG 2 cut(s) 290, 1847
BsaWI WCCGGW 2 cut(s) 46, 341
BsaXI ACNNNNNCTCC 4 cut(s) 347, 377, 952, 982
Bsc4I CCNNNNNNNGG 6 cut(s) 23, 290, 488, 1088, 1750, 2776
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 8 cut(s) 145, 157, 841, 1271, 1342, 1390, 2780, 2809
Bse3DI GCAATG 4 cut(s) 578, 1470, 2679, 2907
BseBI CCWGG 1 cut(s) 1089
BseCI ATCGAT 1 cut(s) 20
BseDI CCNNGG 2 cut(s) 290, 1847
BseGI GGATG 2 cut(s) 934, 2950
BseLI CCNNNNNNNGG 6 cut(s) 23, 290, 488, 1088, 1750, 2776
BseMI GCAATG 4 cut(s) 578, 1470, 2679, 2907
BseMII CTCAG 3 cut(s) 429, 660, 2249
BseNI ACTGG 8 cut(s) 145, 157, 841, 1271, 1342, 1390, 2780, 2809
BseRI GAGGAG 3 cut(s) 569, 973, 1325
BseXI GCAGC 8 cut(s) 649, 1184, 1230, 1712, 1924, 2445, 2674, 2696
BseYI CCCAGC 2 cut(s) 2077, 2944
BsgI GTGCAG 1 cut(s) 1931
BshFI GGCC 6 cut(s) 1104, 1575, 2042, 2083, 2159, 2961
BshTI ACCGGT 1 cut(s) 341
BshVI ATCGAT 1 cut(s) 20
BsiHKAI GWGCWC 3 cut(s) 1241, 1822, 2614
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 5 cut(s) 47, 291, 342, 590, 659
BslI CCNNNNNNNGG 6 cut(s) 23, 290, 488, 1088, 1750, 2776
BsmAI GTCTC 3 cut(s) 367, 1290, 2558
BsmBI CGTCTC 1 cut(s) 367
BsmI GAATGC 1 cut(s) 172
BsnI GGCC 6 cut(s) 1104, 1575, 2042, 2083, 2159, 2961
Bso31I GGTCTC 1 cut(s) 1290
BsoBI CYCGRG 1 cut(s) 290
Bsp1286I GDGCHC 4 cut(s) 17, 1241, 1822, 2614
Bsp1407I TGTACA 1 cut(s) 1177
Bsp143I GATC 8 cut(s) 294, 790, 1018, 1690, 2002, 2221, 2254, 2797
Bsp1720I GCTNAGC 1 cut(s) 921
Bsp19I CCATGG 1 cut(s) 1847
BspACI CCGC 5 cut(s) 110, 200, 629, 632, 862
BspANI GGCC 6 cut(s) 1104, 1575, 2042, 2083, 2159, 2961
BspCNI CTCAG 3 cut(s) 430, 661, 2250
BspDI ATCGAT 1 cut(s) 20
BspHI TCATGA 2 cut(s) 1822, 2703
BspLI GGNNCC 3 cut(s) 2082, 2481, 2911
BspMI ACCTGC 1 cut(s) 1738
BspPI GGATC 3 cut(s) 302, 798, 2792
BspTI CTTAAG 2 cut(s) 455, 1478
BspTNI GGTCTC 1 cut(s) 1290
BsrDI GCAATG 4 cut(s) 578, 1470, 2679, 2907
BsrFI RCCGGY 1 cut(s) 341
BsrGI TGTACA 1 cut(s) 1177
BsrI ACTGG 8 cut(s) 145, 157, 841, 1271, 1342, 1390, 2780, 2809
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 2 cut(s) 290, 1847
BssMI GATC 8 cut(s) 294, 790, 1018, 1690, 2002, 2221, 2254, 2797
BssT1I CCWWGG 1 cut(s) 1847
Bst2UI CCWGG 1 cut(s) 1089
Bst4CI ACNGT 5 cut(s) 125, 376, 448, 1028, 2720
Bst6I CTCTTC 3 cut(s) 2304, 2393, 2775
BstAFI CTTAAG 2 cut(s) 455, 1478
BstAUI TGTACA 1 cut(s) 1177
BstBAI YACGTR 1 cut(s) 1559
BstC8I GCNNGC 6 cut(s) 595, 1120, 1241, 2075, 2145, 2980
BstDEI CTNAG 8 cut(s) 438, 465, 669, 921, 1371, 1404, 2258, 2394
BstDSI CCRYGG 1 cut(s) 1847
BstEII GGTNACC 1 cut(s) 42
BstF5I GGATG 2 cut(s) 934, 2950
BstH2I RGCGCY 1 cut(s) 975
BstHHI GCGC 1 cut(s) 974
BstKTI GATC 8 cut(s) 297, 793, 1021, 1693, 2005, 2224, 2257, 2800
BstMAI GTCTC 3 cut(s) 367, 1290, 2558
BstMBI GATC 8 cut(s) 294, 790, 1018, 1690, 2002, 2221, 2254, 2797
BstMWI GCNNNNNNNGC 3 cut(s) 34, 671, 1581
BstNI CCWGG 1 cut(s) 1089
BstNSI RCATGY 4 cut(s) 1122, 1193, 1440, 2077
BstPI GGTNACC 1 cut(s) 42
BstSCI CCNGG 3 cut(s) 289, 290, 1087
BstSFI CTRYAG 2 cut(s) 1155, 2355
BstV1I GCAGC 8 cut(s) 649, 1184, 1230, 1712, 1924, 2445, 2674, 2696
BstV2I GAAGAC 2 cut(s) 1340, 2357
BstX2I RGATCY 1 cut(s) 294
BstXI CCANNNNNNTGG 1 cut(s) 2957
BstYI RGATCY 1 cut(s) 294
Bsu15I ATCGAT 1 cut(s) 20
BsuI GTATCC 1 cut(s) 523
BsuRI GGCC 6 cut(s) 1104, 1575, 2042, 2083, 2159, 2961
BsuTUI ATCGAT 1 cut(s) 20
BtgI CCRYGG 1 cut(s) 1847
BtsCI GGATG 2 cut(s) 934, 2950
BtsIMutI CAGTG 6 cut(s) 150, 381, 750, 922, 1822, 2773
BveI ACCTGC 1 cut(s) 1738
Cac8I GCNNGC 6 cut(s) 595, 1120, 1241, 2075, 2145, 2980
CciI TCATGA 2 cut(s) 1822, 2703
CfoI GCGC 1 cut(s) 974
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 4 cut(s) 2081, 2157, 2909, 2960
Cfr9I CCCGGG 1 cut(s) 290
ClaI ATCGAT 1 cut(s) 20
Csp6I GTAC 9 cut(s) 144, 786, 1178, 1556, 1884, 1986, 2062, 2639, 2716
CspAI ACCGGT 1 cut(s) 341
CspCI CAANNNNNGTGG 2 cut(s) 1081, 1116
CviQI GTAC 9 cut(s) 144, 786, 1178, 1556, 1884, 1986, 2062, 2639, 2716
DdeI CTNAG 8 cut(s) 438, 465, 669, 921, 1371, 1404, 2258, 2394
DpnI GATC 8 cut(s) 296, 792, 1020, 1692, 2004, 2223, 2256, 2799
DpnII GATC 8 cut(s) 294, 790, 1018, 1690, 2002, 2221, 2254, 2797
DraI TTTAAA 1 cut(s) 2766
EaeI YGGCCR 2 cut(s) 1573, 2040
Eam1104I CTCTTC 3 cut(s) 2304, 2393, 2775
EarI CTCTTC 3 cut(s) 2304, 2393, 2775
EciI GGCGGA 1 cut(s) 618
Ecl136II GAGCTC 2 cut(s) 1239, 2612
Eco130I CCWWGG 1 cut(s) 1847
Eco24I GRGCYC 3 cut(s) 17, 1241, 2614
Eco31I GGTCTC 1 cut(s) 1290
Eco47I GGWCC 1 cut(s) 2909
Eco47III AGCGCT 1 cut(s) 973
Eco53kI GAGCTC 2 cut(s) 1239, 2612
Eco57I CTGAAG 2 cut(s) 1248, 2109
Eco88I CYCGRG 1 cut(s) 290
Eco91I GGTNACC 1 cut(s) 42
EcoICRI GAGCTC 2 cut(s) 1239, 2612
EcoO109I RGGNCCY 1 cut(s) 2909
EcoO65I GGTNACC 1 cut(s) 42
EcoRI GAATTC 2 cut(s) 545, 2469
EcoRII CCWGG 1 cut(s) 1087
EcoT14I CCWWGG 1 cut(s) 1847
EcoT22I ATGCAT 1 cut(s) 1124
EcoT38I GRGCYC 3 cut(s) 17, 1241, 2614
ErhI CCWWGG 1 cut(s) 1847
Esp3I CGTCTC 1 cut(s) 367
FauI CCCGC 1 cut(s) 855
FauNDI CATATG 3 cut(s) 723, 2029, 2600
FbaI TGATCA 3 cut(s) 1018, 2002, 2221
FblI GTMKAC 1 cut(s) 641
Fnu4HI GCNGC 9 cut(s) 632, 663, 1173, 1244, 1701, 1913, 2459, 2685, 2688
FokI GGATG 2 cut(s) 941, 2937
FriOI GRGCYC 3 cut(s) 17, 1241, 2614
Fsp4HI GCNGC 9 cut(s) 632, 663, 1173, 1244, 1701, 1913, 2459, 2685, 2688
FspBI CTAG 6 cut(s) 87, 783, 1293, 1581, 1868, 2607
GlaI GCGC 1 cut(s) 973
GluI GCNGC 9 cut(s) 632, 663, 1173, 1244, 1701, 1913, 2459, 2685, 2688
GsaI CCCAGC 2 cut(s) 2081, 2948
GsuI CTGGAG 1 cut(s) 2763
HaeII RGCGCY 1 cut(s) 975
HaeIII GGCC 6 cut(s) 1104, 1575, 2042, 2083, 2159, 2961
HapII CCGG 5 cut(s) 47, 291, 342, 590, 659
HhaI GCGC 1 cut(s) 974
Hin6I GCGC 1 cut(s) 972
HinP1I GCGC 1 cut(s) 972
HincII GTYRAC 1 cut(s) 642
HindII GTYRAC 1 cut(s) 642
HindIII AAGCTT 2 cut(s) 452, 2141
HpaII CCGG 5 cut(s) 47, 291, 342, 590, 659
Hpy166II GTNNAC 3 cut(s) 228, 642, 1343
Hpy188I TCNGA 9 cut(s) 12, 496, 544, 1023, 1134, 1257, 1309, 1798, 2259
Hpy188III TCNNGA 7 cut(s) 1606, 1823, 1868, 2561, 2607, 2704, 2845
Hpy8I GTNNAC 3 cut(s) 228, 642, 1343
Hpy99I CGWCG 3 cut(s) 103, 640, 643
HpyAV CCTTC 6 cut(s) 312, 417, 544, 1546, 1826, 2133
HpyCH4III ACNGT 5 cut(s) 125, 376, 448, 1028, 2720
HpyCH4IV ACGT 2 cut(s) 1558, 2587
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 671, 1581
HpyF3I CTNAG 8 cut(s) 438, 465, 669, 921, 1371, 1404, 2258, 2394
HpySE526I ACGT 2 cut(s) 1558, 2587
HspAI GCGC 1 cut(s) 972
Ksp22I TGATCA 3 cut(s) 1018, 2002, 2221
Kzo9I GATC 8 cut(s) 294, 790, 1018, 1690, 2002, 2221, 2254, 2797
LmnI GCTCC 5 cut(s) 49, 409, 556, 1937, 2180
Lsp1109I GCAGC 8 cut(s) 649, 1184, 1230, 1712, 1924, 2445, 2674, 2696
MaeI CTAG 6 cut(s) 87, 783, 1293, 1581, 1868, 2607
MaeII ACGT 2 cut(s) 1558, 2587
MaeIII GTNAC 4 cut(s) 42, 337, 2329, 2746
MalI GATC 8 cut(s) 296, 792, 1020, 1692, 2004, 2223, 2256, 2799
MboI GATC 8 cut(s) 294, 790, 1018, 1690, 2002, 2221, 2254, 2797
MfeI CAATTG 2 cut(s) 1203, 2286
MflI RGATCY 1 cut(s) 294
MhlI GDGCHC 4 cut(s) 17, 1241, 1822, 2614
MlsI TGGCCA 2 cut(s) 1575, 2042
MluNI TGGCCA 2 cut(s) 1575, 2042
MlyI GAGTC 3 cut(s) 1027, 1504, 2848
MmeI TCCRAC 3 cut(s) 96, 1313, 2825
Mox20I TGGCCA 2 cut(s) 1575, 2042
Mph1103I ATGCAT 1 cut(s) 1124
MroXI GAANNNNTTC 3 cut(s) 1737, 1840, 1863
MscI TGGCCA 2 cut(s) 1575, 2042
MslI CAYNNNNRTG 7 cut(s) 324, 753, 755, 1431, 2373, 2736, 2955
Msp20I TGGCCA 2 cut(s) 1575, 2042
MspA1I CMGCKG 4 cut(s) 1175, 1279, 1703, 2687
MspCI CTTAAG 2 cut(s) 455, 1478
MspI CCGG 5 cut(s) 47, 291, 342, 590, 659
MspR9I CCNGG 3 cut(s) 291, 292, 1089
MunI CAATTG 2 cut(s) 1203, 2286
Mva1269I GAATGC 1 cut(s) 172
MvaI CCWGG 1 cut(s) 1089
MwoI GCNNNNNNNGC 3 cut(s) 34, 671, 1581
NciI CCSGG 2 cut(s) 291, 292
NcoI CCATGG 1 cut(s) 1847
NdeI CATATG 3 cut(s) 723, 2029, 2600
NdeII GATC 8 cut(s) 294, 790, 1018, 1690, 2002, 2221, 2254, 2797
NlaIV GGNNCC 3 cut(s) 2082, 2481, 2911
NmuCI GTSAC 2 cut(s) 42, 2746
NsiI ATGCAT 1 cut(s) 1124
NspI RCATGY 4 cut(s) 1122, 1193, 1440, 2077
OliI CACNNNNGTG 1 cut(s) 755
PaeI GCATGC 2 cut(s) 1122, 2077
PagI TCATGA 2 cut(s) 1822, 2703
PaqCI CACCTGC 1 cut(s) 1738
PcsI WCGNNNNNNNCGW 1 cut(s) 62
PctI GAATGC 1 cut(s) 172
PdmI GAANNNNTTC 3 cut(s) 1737, 1840, 1863
PfeI GAWTC 9 cut(s) 275, 509, 655, 1220, 1738, 1791, 1836, 2193, 2489
PflMI CCANNNNNTGG 2 cut(s) 23, 1750
PinAI ACCGGT 1 cut(s) 341
PkrI GCNGC 9 cut(s) 633, 664, 1174, 1245, 1702, 1914, 2460, 2686, 2689
PleI GAGTC 3 cut(s) 1027, 1503, 2848
PpsI GAGTC 3 cut(s) 1027, 1503, 2848
Ppu21I YACGTR 1 cut(s) 1559
PpuMI RGGWCCY 1 cut(s) 2909
PshBI ATTAAT 1 cut(s) 1509
Psp124BI GAGCTC 2 cut(s) 1241, 2614
Psp5II RGGWCCY 1 cut(s) 2909
Psp6I CCWGG 1 cut(s) 1087
PspEI GGTNACC 1 cut(s) 42
PspFI CCCAGC 2 cut(s) 2077, 2944
PspGI CCWGG 1 cut(s) 1087
PspN4I GGNNCC 3 cut(s) 2082, 2481, 2911
PspPI GGNCC 4 cut(s) 2081, 2157, 2909, 2960
PspPPI RGGWCCY 1 cut(s) 2909
PsuI RGATCY 1 cut(s) 294
PvuII CAGCTG 4 cut(s) 1175, 1279, 1703, 2687
RsaI GTAC 9 cut(s) 145, 787, 1179, 1557, 1885, 1987, 2063, 2640, 2717
RsaNI GTAC 9 cut(s) 144, 786, 1178, 1556, 1884, 1986, 2062, 2639, 2716
RseI CAYNNNNRTG 7 cut(s) 324, 753, 755, 1431, 2373, 2736, 2955
SacI GAGCTC 2 cut(s) 1241, 2614
SalI GTCGAC 1 cut(s) 640
SatI GCNGC 9 cut(s) 632, 663, 1173, 1244, 1701, 1913, 2459, 2685, 2688
Sau3AI GATC 8 cut(s) 294, 790, 1018, 1690, 2002, 2221, 2254, 2797
Sau96I GGNCC 4 cut(s) 2081, 2157, 2909, 2960
ScaI AGTACT 1 cut(s) 2063
SchI GAGTC 3 cut(s) 1027, 1504, 2848
ScrFI CCNGG 3 cut(s) 291, 292, 1089
SduI GDGCHC 4 cut(s) 17, 1241, 1822, 2614
SfcI CTRYAG 2 cut(s) 1155, 2355
SinI GGWCC 1 cut(s) 2909
SmaI CCCGGG 1 cut(s) 292
SmiMI CAYNNNNRTG 7 cut(s) 324, 753, 755, 1431, 2373, 2736, 2955
SmlI CTYRAG 5 cut(s) 455, 905, 1161, 1478, 2870
SmoI CTYRAG 5 cut(s) 455, 905, 1161, 1478, 2870
SpeI ACTAGT 1 cut(s) 782
SphI GCATGC 2 cut(s) 1122, 2077
SsiI CCGC 5 cut(s) 110, 200, 629, 632, 862
SspI AATATT 6 cut(s) 1048, 1862, 1906, 1925, 1933, 2454
SspMI CTAG 6 cut(s) 87, 783, 1293, 1581, 1868, 2607
SstI GAGCTC 2 cut(s) 1241, 2614
StyD4I CCNGG 3 cut(s) 289, 290, 1087
StyI CCWWGG 1 cut(s) 1847
TaaI ACNGT 5 cut(s) 125, 376, 448, 1028, 2720
TaiI ACGT 2 cut(s) 1561, 2590
TaqI TCGA 7 cut(s) 20, 305, 524, 641, 1031, 2136, 2560
TatI WGTACW 5 cut(s) 1177, 1883, 1985, 2061, 2715
TauI GCSGC 1 cut(s) 634
TfiI GAWTC 9 cut(s) 275, 509, 655, 1220, 1738, 1791, 1836, 2193, 2489
TscAI CASTG 6 cut(s) 157, 381, 757, 922, 1822, 2780
TseFI GTSAC 2 cut(s) 42, 2746
TseI GCWGC 8 cut(s) 662, 1172, 1243, 1700, 1912, 2458, 2684, 2687
Tsp45I GTSAC 2 cut(s) 42, 2746
TspGWI ACGGA 2 cut(s) 803, 1213
TspMI CCCGGG 1 cut(s) 290
TspRI CASTG 6 cut(s) 157, 381, 757, 922, 1822, 2780
Van91I CCANNNNNTGG 2 cut(s) 23, 1750
Vha464I CTTAAG 2 cut(s) 455, 1478
VpaK11BI GGWCC 1 cut(s) 2909
VspI ATTAAT 1 cut(s) 1509
XapI RAATTY 8 cut(s) 545, 739, 826, 1039, 1595, 2230, 2469, 2555
XbaI TCTAGA 2 cut(s) 1867, 2606
XceI RCATGY 4 cut(s) 1122, 1193, 1440, 2077
XcmI CCANNNNNNNNNTGG 2 cut(s) 728, 1278
XmaI CCCGGG 1 cut(s) 290
XmiI GTMKAC 1 cut(s) 641
XmnI GAANNNNTTC 3 cut(s) 1737, 1840, 1863
XspI CTAG 6 cut(s) 87, 783, 1293, 1581, 1868, 2607
ZrmI AGTACT 1 cut(s) 2063
Zsp2I ATGCAT 1 cut(s) 1124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.