Belongs to the eIF-2B alpha beta delta subunits family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 146 |
| AccI | GTMKAC | 1 cut(s) | 506 |
| AciI | CCGC | 3 cut(s) | 280, 538, 541 |
| AcsI | RAATTY | 1 cut(s) | 387 |
| AfaI | GTAC | 1 cut(s) | 27 |
| AgsI | TTSAA | 3 cut(s) | 184, 215, 499 |
| AluBI | AGCT | 4 cut(s) | 101, 113, 242, 307 |
| AluI | AGCT | 4 cut(s) | 101, 113, 242, 307 |
| ApeKI | GCWGC | 3 cut(s) | 225, 239, 317 |
| ApoI | RAATTY | 1 cut(s) | 387 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 270, 352, 375, 387 |
| AspS9I | GGNCC | 1 cut(s) | 169 |
| AsuHPI | GGTGA | 2 cut(s) | 466, 545 |
| AvaII | GGWCC | 1 cut(s) | 169 |
| BbvI | GCAGC | 3 cut(s) | 237, 251, 329 |
| BfaI | CTAG | 3 cut(s) | 222, 314, 333 |
| BfmI | CTRYAG | 2 cut(s) | 57, 557 |
| BisI | GCNGC | 4 cut(s) | 226, 240, 318, 541 |
| BlsI | GCNGC | 4 cut(s) | 227, 241, 319, 542 |
| Bme18I | GGWCC | 1 cut(s) | 169 |
| BmgT120I | GGNCC | 1 cut(s) | 169 |
| BmsI | GCATC | 1 cut(s) | 199 |
| BsaJI | CCNNGG | 1 cut(s) | 359 |
| BseDI | CCNNGG | 1 cut(s) | 359 |
| BseGI | GGATG | 3 cut(s) | 190, 370, 469 |
| BseMII | CTCAG | 2 cut(s) | 186, 240 |
| BseRI | GAGGAG | 2 cut(s) | 266, 473 |
| BseXI | GCAGC | 3 cut(s) | 237, 251, 329 |
| Bsp143I | GATC | 1 cut(s) | 133 |
| Bsp19I | CCATGG | 1 cut(s) | 359 |
| BspACI | CCGC | 3 cut(s) | 280, 538, 541 |
| BspCNI | CTCAG | 2 cut(s) | 187, 241 |
| BspQI | GCTCTTC | 1 cut(s) | 76 |
| BssECI | CCNNGG | 1 cut(s) | 359 |
| BssMI | GATC | 1 cut(s) | 133 |
| BssT1I | CCWWGG | 1 cut(s) | 359 |
| Bst4CI | ACNGT | 2 cut(s) | 449, 511 |
| Bst6I | CTCTTC | 1 cut(s) | 76 |
| BstDEI | CTNAG | 2 cut(s) | 195, 249 |
| BstDSI | CCRYGG | 1 cut(s) | 359 |
| BstEII | GGTNACC | 1 cut(s) | 533 |
| BstF5I | GGATG | 3 cut(s) | 190, 370, 469 |
| BstKTI | GATC | 1 cut(s) | 136 |
| BstMBI | GATC | 1 cut(s) | 133 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 277 |
| BstPI | GGTNACC | 1 cut(s) | 533 |
| BstSFI | CTRYAG | 2 cut(s) | 57, 557 |
| BstV1I | GCAGC | 3 cut(s) | 237, 251, 329 |
| BtgI | CCRYGG | 1 cut(s) | 359 |
| BtsCI | GGATG | 3 cut(s) | 190, 370, 469 |
| BtsIMutI | CAGTG | 1 cut(s) | 334 |
| Cfr13I | GGNCC | 1 cut(s) | 169 |
| CseI | GACGC | 1 cut(s) | 149 |
| Csp6I | GTAC | 1 cut(s) | 26 |
| CviAII | CATG | 3 cut(s) | 23, 360, 454 |
| CviJI | RGCY | 6 cut(s) | 67, 101, 113, 228, 242, 307 |
| CviKI_1 | RGCY | 6 cut(s) | 67, 101, 113, 228, 242, 307 |
| CviQI | GTAC | 1 cut(s) | 26 |
| DdeI | CTNAG | 2 cut(s) | 195, 249 |
| DpnI | GATC | 1 cut(s) | 135 |
| DpnII | GATC | 1 cut(s) | 133 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 146 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 146 |
| Eam1104I | CTCTTC | 1 cut(s) | 76 |
| EarI | CTCTTC | 1 cut(s) | 76 |
| Eco130I | CCWWGG | 1 cut(s) | 359 |
| Eco47I | GGWCC | 1 cut(s) | 169 |
| Eco91I | GGTNACC | 1 cut(s) | 533 |
| EcoO109I | RGGNCCY | 1 cut(s) | 169 |
| EcoO65I | GGTNACC | 1 cut(s) | 533 |
| EcoT14I | CCWWGG | 1 cut(s) | 359 |
| ErhI | CCWWGG | 1 cut(s) | 359 |
| FaeI | CATG | 3 cut(s) | 26, 363, 457 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 255, 287 |
| FatI | CATG | 3 cut(s) | 22, 359, 453 |
| FauI | CCCGC | 1 cut(s) | 273 |
| FblI | GTMKAC | 1 cut(s) | 506 |
| Fnu4HI | GCNGC | 4 cut(s) | 226, 240, 318, 541 |
| FokI | GGATG | 3 cut(s) | 177, 377, 476 |
| Fsp4HI | GCNGC | 4 cut(s) | 226, 240, 318, 541 |
| FspBI | CTAG | 3 cut(s) | 222, 314, 333 |
| GluI | GCNGC | 4 cut(s) | 226, 240, 318, 541 |
| HgaI | GACGC | 1 cut(s) | 149 |
| Hin1II | CATG | 3 cut(s) | 26, 363, 457 |
| HincII | GTYRAC | 1 cut(s) | 507 |
| HindII | GTYRAC | 1 cut(s) | 507 |
| HinfI | GANTC | 4 cut(s) | 199, 245, 324, 348 |
| HphI | GGTGA | 2 cut(s) | 466, 545 |
| Hpy166II | GTNNAC | 1 cut(s) | 507 |
| Hpy188I | TCNGA | 3 cut(s) | 11, 250, 393 |
| Hpy188III | TCNNGA | 4 cut(s) | 137, 181, 194, 380 |
| Hpy8I | GTNNAC | 1 cut(s) | 507 |
| HpyAV | CCTTC | 1 cut(s) | 338 |
| HpyCH4III | ACNGT | 2 cut(s) | 449, 511 |
| HpyCH4V | TGCA | 4 cut(s) | 4, 206, 239, 445 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 277 |
| HpyF3I | CTNAG | 2 cut(s) | 195, 249 |
| Hsp92II | CATG | 3 cut(s) | 26, 363, 457 |
| Kzo9I | GATC | 1 cut(s) | 133 |
| LguI | GCTCTTC | 1 cut(s) | 76 |
| LmnI | GCTCC | 2 cut(s) | 40, 253 |
| LpnPI | CCDG | 4 cut(s) | 207, 306, 393, 420 |
| Lsp1109I | GCAGC | 3 cut(s) | 237, 251, 329 |
| LweI | GCATC | 1 cut(s) | 199 |
| MaeI | CTAG | 3 cut(s) | 222, 314, 333 |
| MaeIII | GTNAC | 1 cut(s) | 533 |
| MalI | GATC | 1 cut(s) | 135 |
| MboI | GATC | 1 cut(s) | 133 |
| MboII | GAAGA | 4 cut(s) | 93, 380, 395, 536 |
| MfeI | CAATTG | 1 cut(s) | 234 |
| MluCI | AATT | 5 cut(s) | 234, 266, 352, 387, 480 |
| MlyI | GAGTC | 1 cut(s) | 208 |
| MnlI | CCTC | 8 cut(s) | 160, 182, 244, 292, 331, 429, 451, 454 |
| MroXI | GAANNNNTTC | 4 cut(s) | 270, 352, 375, 387 |
| MspA1I | CMGCKG | 2 cut(s) | 242, 280 |
| MunI | CAATTG | 1 cut(s) | 234 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 277 |
| NcoI | CCATGG | 1 cut(s) | 359 |
| NdeII | GATC | 1 cut(s) | 133 |
| NlaIII | CATG | 3 cut(s) | 26, 363, 457 |
| NmuCI | GTSAC | 1 cut(s) | 533 |
| PciSI | GCTCTTC | 1 cut(s) | 76 |
| PdmI | GAANNNNTTC | 4 cut(s) | 270, 352, 375, 387 |
| PfeI | GAWTC | 3 cut(s) | 245, 324, 348 |
| PkrI | GCNGC | 4 cut(s) | 227, 241, 319, 542 |
| PleI | GAGTC | 1 cut(s) | 207 |
| PpsI | GAGTC | 1 cut(s) | 207 |
| PpuMI | RGGWCCY | 1 cut(s) | 169 |
| Psp5II | RGGWCCY | 1 cut(s) | 169 |
| PspEI | GGTNACC | 1 cut(s) | 533 |
| PspPI | GGNCC | 1 cut(s) | 169 |
| PspPPI | RGGWCCY | 1 cut(s) | 169 |
| PvuII | CAGCTG | 1 cut(s) | 242 |
| RsaI | GTAC | 1 cut(s) | 27 |
| RsaNI | GTAC | 1 cut(s) | 26 |
| SalI | GTCGAC | 1 cut(s) | 505 |
| SapI | GCTCTTC | 1 cut(s) | 76 |
| SatI | GCNGC | 4 cut(s) | 226, 240, 318, 541 |
| Sau3AI | GATC | 1 cut(s) | 133 |
| Sau96I | GGNCC | 1 cut(s) | 169 |
| SchI | GAGTC | 1 cut(s) | 208 |
| SetI | ASST | 8 cut(s) | 103, 115, 171, 244, 303, 309, 342, 427 |
| SfaNI | GCATC | 1 cut(s) | 199 |
| SfcI | CTRYAG | 2 cut(s) | 57, 557 |
| SinI | GGWCC | 1 cut(s) | 169 |
| Sse9I | AATT | 5 cut(s) | 234, 266, 352, 387, 480 |
| SsiI | CCGC | 3 cut(s) | 280, 538, 541 |
| SspI | AATATT | 1 cut(s) | 430 |
| SspMI | CTAG | 3 cut(s) | 222, 314, 333 |
| StyI | CCWWGG | 1 cut(s) | 359 |
| TaaI | ACNGT | 2 cut(s) | 449, 511 |
| TaqI | TCGA | 1 cut(s) | 506 |
| TasI | AATT | 5 cut(s) | 234, 266, 352, 387, 480 |
| TatI | WGTACW | 1 cut(s) | 25 |
| TauI | GCSGC | 1 cut(s) | 543 |
| TfiI | GAWTC | 3 cut(s) | 245, 324, 348 |
| TscAI | CASTG | 1 cut(s) | 334 |
| TseFI | GTSAC | 1 cut(s) | 533 |
| TseI | GCWGC | 3 cut(s) | 225, 239, 317 |
| Tsp45I | GTSAC | 1 cut(s) | 533 |
| TspDTI | ATGAA | 4 cut(s) | 11, 263, 381, 537 |
| TspGWI | ACGGA | 1 cut(s) | 53 |
| TspRI | CASTG | 1 cut(s) | 334 |
| VpaK11BI | GGWCC | 1 cut(s) | 169 |
| XapI | RAATTY | 1 cut(s) | 387 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 484 |
| XmiI | GTMKAC | 1 cut(s) | 506 |
| XmnI | GAANNNNTTC | 4 cut(s) | 270, 352, 375, 387 |
| XspI | CTAG | 3 cut(s) | 222, 314, 333 |