Rroxscaffold_1G00034320

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000779
Physical Location & Seq
Reverse (-)
49800626 .. 49802362
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00034320.1

Sequence Viewer

Length: 936 bp
ATGAAAAAGACATCGAAAAAAGCTGTGAACACGGCCACCGCCAAGTATGGAAAGCTAGACATTATGTTTAACAATGTTGGCATCAGCGACGTGGTCAAGCTTAAAGCTCTGATAGAGACAAGATGCCTTGTACATTTTAGAAGGATCAAATGCAAAGAATTCGAATACGTGCGAGATGATGCGGTTGATCCATTAGAGAAGGGGCATAGTGATGGGTTTGATGATAAGGAAAATGATGTCAAACAATTTGTTTTCGATGAGAATTTAGGCATCTACCAGTCAAGTCATTTAAGTGGAGGCACTGTTGTCTCTTTTAATATGCATGAATTGGAAGCTAGCCTTGAACTGGATGTCAATAGCAACAAGCCTCCTCTAGTCCGTAAAGACTTTGATTCAAAGCAAGCAACCAAACCTGTTGATAATCAGCCCACCCTTGGAAGTTTTTGTGGGGACTCAAGCGGATGTAGCTGGTTTCAAGTCAATTCTATGCTTAAGCATTACATTGAGGGTTTAAAGAAGATTAGCTACTATGTACTGCAACTATGCTTCTCTGTCTTTGATAAGAAGGTTCGTGATAAAGGTGGTGGTCACACTCTATCAACAGATGAATCTTTTATCATGAATCGAGAGTTTACATGGAATATGAGAAATGATGGATACATGGTTGTTCTATGTTCCAACATGTTTAAAGATGGGTTTAATTTGGAGTATCTGTGGGAGACAAAAGGATACGGGGATTTGCTTATCTTTGGCTCACCCTCGCATGTGAAGCTTCTTAAGAATCATGCACTAGTTTCGAATCATTTCTATGTGTTTGTAAAGGAGTTGGAGAAAGCACCTGCTATTTTGCTTCGAGTTTGCTCAAAATTACGGCAGATATTAGAGAGCATATTGAGCTTCGTCATGGGCAAGCGAAAAGGGCGAGTTGTATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

311

Amino Acids

35.48

Weight (kDa)

8.77

Isoelectric Point (pI)

30.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000779)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g29862
rosa_chinensis RchiOBHm_Chr1g0313641 RchiOBHm_Chr4g0408161 RchiOBHm_Chr4g0408171 RchiOBHm_Chr4g0437611 RchiOBHm_Chr4g0437621 RchiOBHm_Chr4g0444671 RchiOBHm_Chr5g0045021 RchiOBHm_Chr5g0070121 RchiOBHm_Chr5g0082551 RchiOBHm_Chr6g0247501 RchiOBHm_Chr6g0271311 RchiOBHm_Chr6g0290811 RchiOBHm_Chr6g0310571
rosa_laevigata RLG00000004718 RLG00000008552 RLG00000015595
rosa_multiflora Rmu_co8342433.1_g000001 Rmu_sc0000147.1_g000080 Rmu_sc0000391.1_g000018 Rmu_sc0000753.1_g000021 Rmu_sc0000870.1_g000071 Rmu_sc0000870.1_g000072 Rmu_sc0001648.1_g000026 Rmu_sc0001648.1_g000043 Rmu_sc0001972.1_g000009 Rmu_sc0002109.1_g000009 Rmu_sc0003340.1_g000008 Rmu_sc0003340.1_g000009 Rmu_sc0004874.1_g000014 Rmu_sc0005823.1_g000009 Rmu_sc0005941.1_g000006 Rmu_sc0009850.1_g000015 Rmu_sc0015511.1_g000012
rosa_roxburghii Rroxscaffold_1G00034320 Rroxscaffold_1G00036130 Rroxscaffold_1G00043440 Rroxscaffold_1G00043450 Rroxscaffold_1G00050660 Rroxscaffold_1G00050670 Rroxscaffold_1G00066940 Rroxscaffold_2G00094100 Rroxscaffold_2G00120240 Rroxscaffold_4G00287900 Rroxscaffold_4G00287910 Rroxscaffold_4G00290700 Rroxscaffold_4G00290710 Rroxscaffold_4G00298640 Rroxscaffold_4G00308540 Rroxscaffold_5G00369420 Rroxscaffold_7G00217900
rosa_rugosa Rorug06G0077100
rosa_samantha Rh1AG005600 Rh1AG005700 Rh1BG433000 Rh1DG003900 Rh2CG286600 Rh3AG243900 Rh3BG368800 Rh5AG301800 Rh5BG309500 Rh5CG501500 Rh5CG501600 Rh5DG320200 Rh6AG179500 Rh6CG338800 Rh6CG405000 Rh6DG092100 Rh7AG294100 Rh7CG239600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 847
Acc36I ACCTGC 1 cut(s) 847
AciI CCGC 3 cut(s) 39, 182, 459
AclWI GGATC 2 cut(s) 152, 182
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 2 cut(s) 158, 262
AfaI GTAC 2 cut(s) 132, 534
AfiI CCNNNNNNNGG 2 cut(s) 346, 434
AflII CTTAAG 2 cut(s) 491, 776
AflIII ACRYGT 1 cut(s) 681
AgsI TTSAA 3 cut(s) 344, 396, 476
AhlI ACTAGT 1 cut(s) 790
AjiI CACGTC 1 cut(s) 91
AluBI AGCT 9 cut(s) 23, 55, 100, 107, 335, 468, 525, 772, 897
AluI AGCT 9 cut(s) 23, 55, 100, 107, 335, 468, 525, 772, 897
Alw26I GTCTC 3 cut(s) 110, 313, 713
AlwI GGATC 2 cut(s) 152, 182
AoxI GGCC 1 cut(s) 33
ApoI RAATTY 2 cut(s) 158, 262
Asp700I GAANNNNTTC 1 cut(s) 803
AsuHPI GGTGA 1 cut(s) 747
AsuII TTCGAA 2 cut(s) 162, 797
AsuNHI GCTAGC 1 cut(s) 335
BarI GAAGNNNNNNTAC 2 cut(s) 509, 541
BccI CCATC 3 cut(s) 206, 647, 686
BceAI ACGGC 2 cut(s) 48, 887
BcgI CGANNNNNNTGC 4 cut(s) 142, 176, 777, 811
BciVI GTATCC 2 cut(s) 650, 722
BcoDI GTCTC 3 cut(s) 110, 313, 713
BcuI ACTAGT 1 cut(s) 790
BfaI CTAG 4 cut(s) 56, 336, 374, 791
BfrI CTTAAG 2 cut(s) 491, 776
BfuAI ACCTGC 1 cut(s) 847
BfuI GTATCC 2 cut(s) 650, 722
BmgBI CACGTC 1 cut(s) 91
BmsI GCATC 4 cut(s) 90, 113, 169, 279
BmtI GCTAGC 1 cut(s) 339
Bpu14I TTCGAA 2 cut(s) 162, 797
BpuEI CTTGAG 1 cut(s) 439
BsaAI YACGTR 1 cut(s) 169
BsaJI CCNNGG 1 cut(s) 433
BsaXI ACNNNNNCTCC 4 cut(s) 288, 318, 698, 728
Bsc4I CCNNNNNNNGG 2 cut(s) 346, 434
Bse1I ACTGG 2 cut(s) 277, 351
BseDI CCNNGG 1 cut(s) 433
BseGI GGATG 2 cut(s) 355, 467
BseLI CCNNNNNNNGG 2 cut(s) 346, 434
BseNI ACTGG 2 cut(s) 277, 351
BseRI GAGGAG 1 cut(s) 360
BshFI GGCC 1 cut(s) 35
BslFI GGGAC 1 cut(s) 464
BslI CCNNNNNNNGG 2 cut(s) 346, 434
BsmAI GTCTC 3 cut(s) 110, 313, 713
BsmFI GGGAC 1 cut(s) 464
BsnI GGCC 1 cut(s) 35
Bsp119I TTCGAA 2 cut(s) 162, 797
Bsp1407I TGTACA 1 cut(s) 130
Bsp143I GATC 2 cut(s) 144, 187
BspACI CCGC 3 cut(s) 39, 182, 459
BspANI GGCC 1 cut(s) 35
BspHI TCATGA 1 cut(s) 618
BspMI ACCTGC 1 cut(s) 847
BspOI GCTAGC 1 cut(s) 339
BspPI GGATC 2 cut(s) 152, 182
BspT104I TTCGAA 2 cut(s) 162, 797
BspTI CTTAAG 2 cut(s) 491, 776
BsrGI TGTACA 1 cut(s) 130
BsrI ACTGG 2 cut(s) 277, 351
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 2 cut(s) 144, 187
BssT1I CCWWGG 1 cut(s) 433
Bst4CI ACNGT 1 cut(s) 304
BstAFI CTTAAG 2 cut(s) 491, 776
BstAUI TGTACA 1 cut(s) 130
BstBAI YACGTR 1 cut(s) 169
BstBI TTCGAA 2 cut(s) 162, 797
BstC8I GCNNGC 3 cut(s) 337, 402, 911
BstF5I GGATG 2 cut(s) 355, 467
BstKTI GATC 2 cut(s) 147, 190
BstMAI GTCTC 3 cut(s) 110, 313, 713
BstMBI GATC 2 cut(s) 144, 187
BstMWI GCNNNNNNNGC 4 cut(s) 465, 769, 894, 919
BstNSI RCATGY 2 cut(s) 685, 767
BsuI GTATCC 2 cut(s) 650, 722
BsuRI GGCC 1 cut(s) 35
BtrI CACGTC 1 cut(s) 91
BtsCI GGATG 2 cut(s) 355, 467
BtsIMutI CAGTG 1 cut(s) 300
BveI ACCTGC 1 cut(s) 847
Cac8I GCNNGC 3 cut(s) 337, 402, 911
CciI TCATGA 1 cut(s) 618
Csp6I GTAC 2 cut(s) 131, 533
CviAII CATG 8 cut(s) 323, 619, 636, 661, 682, 764, 785, 904
CviQI GTAC 2 cut(s) 131, 533
DpnI GATC 2 cut(s) 146, 189
DpnII GATC 2 cut(s) 144, 187
DraI TTTAAA 2 cut(s) 513, 688
EaeI YGGCCR 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 433
EcoRI GAATTC 1 cut(s) 158
EcoT14I CCWWGG 1 cut(s) 433
EcoT22I ATGCAT 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 433
FaeI CATG 8 cut(s) 326, 622, 639, 664, 685, 767, 788, 907
FalI AAGNNNNNCTT 2 cut(s) 324, 356
FaqI GGGAC 1 cut(s) 464
FatI CATG 8 cut(s) 322, 618, 635, 660, 681, 763, 784, 903
FokI GGATG 2 cut(s) 362, 474
FspBI CTAG 4 cut(s) 56, 336, 374, 791
HaeIII GGCC 1 cut(s) 35
Hin1II CATG 8 cut(s) 326, 622, 639, 664, 685, 767, 788, 907
HindIII AAGCTT 2 cut(s) 98, 770
HinfI GANTC 6 cut(s) 392, 452, 608, 622, 781, 799
HphI GGTGA 1 cut(s) 747
Hpy166II GTNNAC 2 cut(s) 28, 633
Hpy188I TCNGA 1 cut(s) 111
Hpy188III TCNNGA 3 cut(s) 572, 619, 626
Hpy8I GTNNAC 2 cut(s) 28, 633
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 3 cut(s) 135, 193, 559
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4IV ACGT 2 cut(s) 90, 168
HpyCH4V TGCA 4 cut(s) 153, 322, 538, 788
HpyF10VI GCNNNNNNNGC 4 cut(s) 465, 769, 894, 919
HpySE526I ACGT 2 cut(s) 90, 168
Hsp92II CATG 8 cut(s) 326, 622, 639, 664, 685, 767, 788, 907
Kzo9I GATC 2 cut(s) 144, 187
LpnPI CCDG 5 cut(s) 290, 332, 426, 454, 852
LweI GCATC 4 cut(s) 90, 113, 169, 279
MaeI CTAG 4 cut(s) 56, 336, 374, 791
MaeII ACGT 2 cut(s) 90, 168
MaeIII GTNAC 1 cut(s) 587
MalI GATC 2 cut(s) 146, 189
MboI GATC 2 cut(s) 144, 187
MboII GAAGA 1 cut(s) 529
MluCI AATT 7 cut(s) 158, 245, 262, 326, 481, 700, 866
MlyI GAGTC 1 cut(s) 446
MmeI TCCRAC 2 cut(s) 702, 807
MnlI CCTC 5 cut(s) 290, 378, 381, 499, 769
Mph1103I ATGCAT 1 cut(s) 324
MroXI GAANNNNTTC 1 cut(s) 803
MseI TTAA 9 cut(s) 69, 102, 290, 315, 492, 512, 687, 699, 777
MslI CAYNNNNRTG 3 cut(s) 210, 291, 807
MspCI CTTAAG 2 cut(s) 491, 776
MwoI GCNNNNNNNGC 4 cut(s) 465, 769, 894, 919
NdeII GATC 2 cut(s) 144, 187
NheI GCTAGC 1 cut(s) 335
NlaIII CATG 8 cut(s) 326, 622, 639, 664, 685, 767, 788, 907
NmuCI GTSAC 1 cut(s) 587
NsiI ATGCAT 1 cut(s) 324
NspI RCATGY 2 cut(s) 685, 767
NspV TTCGAA 2 cut(s) 162, 797
PagI TCATGA 1 cut(s) 618
PaqCI CACCTGC 1 cut(s) 847
PciI ACATGT 1 cut(s) 681
PdmI GAANNNNTTC 1 cut(s) 803
PfeI GAWTC 5 cut(s) 392, 608, 622, 781, 799
PflFI GACNNNGTC 1 cut(s) 92
PleI GAGTC 1 cut(s) 446
PpsI GAGTC 1 cut(s) 446
Ppu21I YACGTR 1 cut(s) 169
PscI ACATGT 1 cut(s) 681
PsyI GACNNNGTC 1 cut(s) 92
RsaI GTAC 2 cut(s) 132, 534
RsaNI GTAC 2 cut(s) 131, 533
RseI CAYNNNNRTG 3 cut(s) 210, 291, 807
SaqAI TTAA 9 cut(s) 69, 102, 290, 315, 492, 512, 687, 699, 777
Sau3AI GATC 2 cut(s) 144, 187
SchI GAGTC 1 cut(s) 446
SfaNI GCATC 4 cut(s) 90, 113, 169, 279
SfuI TTCGAA 2 cut(s) 162, 797
SmiMI CAYNNNNRTG 3 cut(s) 210, 291, 807
SmlI CTYRAG 3 cut(s) 454, 491, 776
SmoI CTYRAG 3 cut(s) 454, 491, 776
SpeI ACTAGT 1 cut(s) 790
Sse9I AATT 7 cut(s) 158, 245, 262, 326, 481, 700, 866
SsiI CCGC 3 cut(s) 39, 182, 459
SspMI CTAG 4 cut(s) 56, 336, 374, 791
StyI CCWWGG 1 cut(s) 433
TaaI ACNGT 1 cut(s) 304
TaiI ACGT 2 cut(s) 93, 171
TaqI TCGA 6 cut(s) 14, 162, 255, 625, 797, 853
TasI AATT 7 cut(s) 158, 245, 262, 326, 481, 700, 866
TatI WGTACW 2 cut(s) 130, 532
TfiI GAWTC 5 cut(s) 392, 608, 622, 781, 799
Tru1I TTAA 9 cut(s) 69, 102, 290, 315, 492, 512, 687, 699, 777
Tru9I TTAA 9 cut(s) 69, 102, 290, 315, 492, 512, 687, 699, 777
TscAI CASTG 1 cut(s) 307
TseFI GTSAC 1 cut(s) 587
Tsp45I GTSAC 1 cut(s) 587
TspDTI ATGAA 4 cut(s) 17, 339, 621, 635
TspGWI ACGGA 1 cut(s) 368
TspRI CASTG 1 cut(s) 307
Tth111I GACNNNGTC 1 cut(s) 92
Vha464I CTTAAG 2 cut(s) 491, 776
XapI RAATTY 2 cut(s) 158, 262
XceI RCATGY 2 cut(s) 685, 767
XmnI GAANNNNTTC 1 cut(s) 803
XspI CTAG 4 cut(s) 56, 336, 374, 791
Zsp2I ATGCAT 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.