Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 2 cut(s) | 140, 178 |
| AjnI | CCWGG | 2 cut(s) | 229, 309 |
| AluBI | AGCT | 1 cut(s) | 163 |
| AluI | AGCT | 1 cut(s) | 163 |
| Alw26I | GTCTC | 2 cut(s) | 5, 293 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 202, 234 |
| BccI | CCATC | 2 cut(s) | 80, 132 |
| BciT130I | CCWGG | 2 cut(s) | 231, 311 |
| BcoDI | GTCTC | 2 cut(s) | 5, 293 |
| BfaI | CTAG | 1 cut(s) | 164 |
| Bme1390I | CCNGG | 2 cut(s) | 231, 311 |
| BmiI | GGNNCC | 1 cut(s) | 227 |
| BmrFI | CCNGG | 2 cut(s) | 231, 311 |
| BmrI | ACTGGG | 1 cut(s) | 19 |
| BmuI | ACTGGG | 1 cut(s) | 19 |
| BsaJI | CCNNGG | 2 cut(s) | 229, 230 |
| Bse1I | ACTGG | 2 cut(s) | 25, 155 |
| Bse3DI | GCAATG | 1 cut(s) | 118 |
| BseBI | CCWGG | 2 cut(s) | 231, 311 |
| BseDI | CCNNGG | 2 cut(s) | 229, 230 |
| BseGI | GGATG | 4 cut(s) | 66, 91, 124, 323 |
| BseMI | GCAATG | 1 cut(s) | 118 |
| BseMII | CTCAG | 1 cut(s) | 265 |
| BseNI | ACTGG | 2 cut(s) | 25, 155 |
| BslFI | GGGAC | 1 cut(s) | 19 |
| BsmAI | GTCTC | 2 cut(s) | 5, 293 |
| BsmBI | CGTCTC | 2 cut(s) | 5, 293 |
| BsmFI | GGGAC | 1 cut(s) | 19 |
| BspCNI | CTCAG | 1 cut(s) | 264 |
| BspLI | GGNNCC | 1 cut(s) | 227 |
| BsrDI | GCAATG | 1 cut(s) | 118 |
| BsrI | ACTGG | 2 cut(s) | 25, 155 |
| BssECI | CCNNGG | 2 cut(s) | 229, 230 |
| Bst2UI | CCWGG | 2 cut(s) | 231, 311 |
| BstDEI | CTNAG | 2 cut(s) | 251, 345 |
| BstF5I | GGATG | 4 cut(s) | 66, 91, 124, 323 |
| BstMAI | GTCTC | 2 cut(s) | 5, 293 |
| BstNI | CCWGG | 2 cut(s) | 231, 311 |
| BstSCI | CCNGG | 2 cut(s) | 229, 309 |
| BtsCI | GGATG | 4 cut(s) | 66, 91, 124, 323 |
| BtsIMutI | CAGTG | 1 cut(s) | 162 |
| CviAII | CATG | 1 cut(s) | 199 |
| CviJI | RGCY | 2 cut(s) | 163, 263 |
| CviKI_1 | RGCY | 2 cut(s) | 163, 263 |
| DdeI | CTNAG | 2 cut(s) | 251, 345 |
| EcoRII | CCWGG | 2 cut(s) | 229, 309 |
| Esp3I | CGTCTC | 2 cut(s) | 5, 293 |
| FaeI | CATG | 1 cut(s) | 202 |
| FaiI | YATR | 2 cut(s) | 56, 200 |
| FaqI | GGGAC | 1 cut(s) | 19 |
| FatI | CATG | 1 cut(s) | 198 |
| FokI | GGATG | 4 cut(s) | 73, 98, 111, 330 |
| FspBI | CTAG | 1 cut(s) | 164 |
| Hin1II | CATG | 1 cut(s) | 202 |
| HinfI | GANTC | 1 cut(s) | 19 |
| Hpy188I | TCNGA | 2 cut(s) | 247, 254 |
| HpyCH4V | TGCA | 1 cut(s) | 329 |
| HpyF3I | CTNAG | 2 cut(s) | 251, 345 |
| Hsp92II | CATG | 1 cut(s) | 202 |
| LpnPI | CCDG | 7 cut(s) | 38, 168, 189, 216, 243, 296, 323 |
| MaeI | CTAG | 1 cut(s) | 164 |
| MaeIII | GTNAC | 1 cut(s) | 208 |
| MboII | GAAGA | 1 cut(s) | 76 |
| MluCI | AATT | 2 cut(s) | 91, 145 |
| MnlI | CCTC | 4 cut(s) | 43, 123, 216, 260 |
| MseI | TTAA | 2 cut(s) | 95, 324 |
| MspR9I | CCNGG | 2 cut(s) | 231, 311 |
| MvaI | CCWGG | 2 cut(s) | 231, 311 |
| NlaIII | CATG | 1 cut(s) | 202 |
| NlaIV | GGNNCC | 1 cut(s) | 227 |
| NmuCI | GTSAC | 1 cut(s) | 208 |
| PasI | CCCWGGG | 1 cut(s) | 230 |
| PfeI | GAWTC | 1 cut(s) | 19 |
| Psp6I | CCWGG | 2 cut(s) | 229, 309 |
| PspGI | CCWGG | 2 cut(s) | 229, 309 |
| PspN4I | GGNNCC | 1 cut(s) | 227 |
| SaqAI | TTAA | 2 cut(s) | 95, 324 |
| ScrFI | CCNGG | 2 cut(s) | 231, 311 |
| SetI | ASST | 4 cut(s) | 135, 165, 227, 252 |
| Sse9I | AATT | 2 cut(s) | 91, 145 |
| SspMI | CTAG | 1 cut(s) | 164 |
| StyD4I | CCNGG | 2 cut(s) | 229, 309 |
| TasI | AATT | 2 cut(s) | 91, 145 |
| TfiI | GAWTC | 1 cut(s) | 19 |
| Tru1I | TTAA | 2 cut(s) | 95, 324 |
| Tru9I | TTAA | 2 cut(s) | 95, 324 |
| TscAI | CASTG | 1 cut(s) | 162 |
| TseFI | GTSAC | 1 cut(s) | 208 |
| Tsp45I | GTSAC | 1 cut(s) | 208 |
| TspDTI | ATGAA | 3 cut(s) | 77, 84, 289 |
| TspGWI | ACGGA | 1 cut(s) | 23 |
| TspRI | CASTG | 1 cut(s) | 162 |
| XspI | CTAG | 1 cut(s) | 164 |