Rh4DG115500

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000721
Physical Location & Seq
Forward (+)
18218440 .. 18232576
14137 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG115500.1

Sequence Viewer

Length: 750 bp
ATGGCAAACCAAAAGCGCTTCCTCTGGTTCATCTTCATTATTTTGTTCAGCCCTGGGATCGAGCCTTCAACGACGAAAGAAGTGATACGAGTACCAGTAGGAGTTGTTCTTAACTTAAATTCCTCAGTGGGAGCTATGGCAGAGAAGTGCATGACCATGGCACTTGATGATTTTTATGCTAAGCATGCTCATTATCAAACAAGACTTTCCCTTCTCACCAGGGATTCAAAGAATGATGTTGTTTCGGCAGCATTTGAAGGGTCCCCGCTGGTATCCTACATGTCGAGGGCAATCTTGAACGTGACTCAAGACAAAAATCAGATGGATAGAATTCAGTCGACTTACTTTGATTACTTTGATAACAAACCTACCTGTGAGGATCAAAGTGCCAAAATTTCTTCAGAGGGTCCAAGTCTTCGTGTCTACAGCTTCGGAGGCCTCTTCATCATCGCAGGTGTGGCCTCCATGGTTGCTCTTTTGATGTACATGTATCACTTCCTTTGCTCTCATTGGCCAGCTTTGCGAACCATTAGTTCTTCTGACAGCACATTTTGGGAGAAATTGGTTGAAGTGGCAAAGCATTTTGACAAGAAAGATCTCAATTCACATCAGTTTAAGCGAAGAGAATCAAGTGTACATGCTTTAGACACTCCTGGTGAAGGTCTGGCAGCTGCACCGGGTGCTAATGATATGCAAAACAACTCAGCAGGAACTCACATAACAGTGGAGAACAATCTTGGACATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

249

Amino Acids

27.8

Weight (kDa)

6.3

Isoelectric Point (pI)

52.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 443
Acc36I ACCTGC 1 cut(s) 443
AccI GTMKAC 2 cut(s) 338, 423
AciI CCGC 1 cut(s) 266
AclWI GGATC 2 cut(s) 65, 387
AcoI YGGCCR 1 cut(s) 512
AcsI RAATTY 3 cut(s) 118, 330, 393
AcuI CTGAAG 1 cut(s) 384
AdeI CACNNNGTG 1 cut(s) 680
AfaI GTAC 3 cut(s) 93, 485, 636
AfeI AGCGCT 1 cut(s) 17
AfiI CCNNNNNNNGG 1 cut(s) 659
AflIII ACRYGT 2 cut(s) 279, 486
AgsI TTSAA 5 cut(s) 69, 228, 257, 298, 569
AjnI CCWGG 3 cut(s) 52, 218, 652
AluBI AGCT 4 cut(s) 134, 429, 518, 671
AluI AGCT 4 cut(s) 134, 429, 518, 671
AlwI GGATC 2 cut(s) 65, 387
Aor51HI AGCGCT 1 cut(s) 17
AoxI GGCC 3 cut(s) 436, 459, 512
ApeKI GCWGC 3 cut(s) 248, 668, 671
ApoI RAATTY 3 cut(s) 118, 330, 393
AspLEI GCGC 1 cut(s) 18
AspS9I GGNCC 2 cut(s) 261, 407
AsuC2I CCSGG 1 cut(s) 678
AsuHPI GGTGA 2 cut(s) 208, 668
AvaII GGWCC 2 cut(s) 261, 407
BalI TGGCCA 1 cut(s) 514
BbsI GAAGAC 1 cut(s) 407
BbvI GCAGC 3 cut(s) 260, 658, 680
BccI CCATC 1 cut(s) 316
BciT130I CCWGG 3 cut(s) 54, 220, 654
BciVI GTATCC 1 cut(s) 283
BcnI CCSGG 1 cut(s) 678
BfmI CTRYAG 1 cut(s) 424
BfoI RGCGCY 1 cut(s) 19
BfuAI ACCTGC 1 cut(s) 443
BfuI GTATCC 1 cut(s) 283
BglII AGATCT 1 cut(s) 595
BisI GCNGC 3 cut(s) 249, 669, 672
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 3 cut(s) 250, 670, 673
Bme1390I CCNGG 4 cut(s) 54, 220, 654, 678
Bme18I GGWCC 2 cut(s) 261, 407
BmgT120I GGNCC 2 cut(s) 261, 407
BmiI GGNNCC 3 cut(s) 262, 263, 408
BmrFI CCNGG 4 cut(s) 54, 220, 654, 678
BpiI GAAGAC 1 cut(s) 407
Bpu1102I GCTNAGC 1 cut(s) 180
BpuEI CTTGAG 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 678
BsaJI CCNNGG 5 cut(s) 52, 53, 156, 219, 465
Bsc4I CCNNNNNNNGG 1 cut(s) 659
Bse1I ACTGG 1 cut(s) 95
BseBI CCWGG 3 cut(s) 54, 220, 654
BseDI CCNNGG 5 cut(s) 52, 53, 156, 219, 465
BseLI CCNNNNNNNGG 1 cut(s) 659
BseMII CTCAG 2 cut(s) 138, 717
BseNI ACTGG 1 cut(s) 95
BseXI GCAGC 3 cut(s) 260, 658, 680
BsgI GTGCAG 1 cut(s) 657
BshFI GGCC 3 cut(s) 438, 461, 514
BsiSI CCGG 1 cut(s) 677
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 1 cut(s) 659
BsmFI GGGAC 1 cut(s) 247
BsnI GGCC 3 cut(s) 438, 461, 514
Bsp1407I TGTACA 2 cut(s) 483, 634
Bsp143I GATC 3 cut(s) 57, 379, 595
Bsp1720I GCTNAGC 1 cut(s) 180
Bsp19I CCATGG 2 cut(s) 156, 465
BspACI CCGC 1 cut(s) 266
BspANI GGCC 3 cut(s) 438, 461, 514
BspCNI CTCAG 2 cut(s) 137, 716
BspLI GGNNCC 3 cut(s) 262, 263, 408
BspMI ACCTGC 1 cut(s) 443
BspPI GGATC 2 cut(s) 65, 387
BsrGI TGTACA 2 cut(s) 483, 634
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 5 cut(s) 52, 53, 156, 219, 465
BssMI GATC 3 cut(s) 57, 379, 595
BssT1I CCWWGG 2 cut(s) 156, 465
Bst2UI CCWGG 3 cut(s) 54, 220, 654
Bst4CI ACNGT 1 cut(s) 724
Bst6I CTCTTC 2 cut(s) 446, 616
BstAPI GCANNNNNTGC 1 cut(s) 680
BstAUI TGTACA 2 cut(s) 483, 634
BstC8I GCNNGC 2 cut(s) 186, 516
BstDEI CTNAG 3 cut(s) 124, 180, 703
BstDSI CCRYGG 2 cut(s) 156, 465
BstENI CCTNNNNNAGG 1 cut(s) 657
BstH2I RGCGCY 1 cut(s) 19
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 3 cut(s) 60, 382, 598
BstMBI GATC 3 cut(s) 57, 379, 595
BstMWI GCNNNNNNNGC 5 cut(s) 185, 435, 458, 520, 680
BstNI CCWGG 3 cut(s) 54, 220, 654
BstNSI RCATGY 4 cut(s) 188, 283, 490, 641
BstSCI CCNGG 4 cut(s) 52, 218, 652, 676
BstSFI CTRYAG 1 cut(s) 424
BstV1I GCAGC 3 cut(s) 260, 658, 680
BstV2I GAAGAC 1 cut(s) 407
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
BsuI GTATCC 1 cut(s) 283
BsuRI GGCC 3 cut(s) 438, 461, 514
BtgI CCRYGG 2 cut(s) 156, 465
BtgZI GCGATG 1 cut(s) 433
BtsIMutI CAGTG 2 cut(s) 132, 729
BveI ACCTGC 1 cut(s) 443
Cac8I GCNNGC 2 cut(s) 186, 516
CfoI GCGC 1 cut(s) 18
Cfr13I GGNCC 2 cut(s) 261, 407
Csp6I GTAC 3 cut(s) 92, 484, 635
CviAII CATG 8 cut(s) 151, 157, 185, 280, 466, 487, 638, 743
CviJI RGCY 9 cut(s) 51, 64, 134, 429, 438, 461, 514, 518, 671
CviKI_1 RGCY 9 cut(s) 51, 64, 134, 429, 438, 461, 514, 518, 671
CviQI GTAC 3 cut(s) 92, 484, 635
DdeI CTNAG 3 cut(s) 124, 180, 703
DpnI GATC 3 cut(s) 59, 381, 597
DpnII GATC 3 cut(s) 57, 379, 595
DraIII CACNNNGTG 1 cut(s) 680
EaeI YGGCCR 1 cut(s) 512
Eam1104I CTCTTC 2 cut(s) 446, 616
EarI CTCTTC 2 cut(s) 446, 616
Eco130I CCWWGG 2 cut(s) 156, 465
Eco147I AGGCCT 1 cut(s) 438
Eco47I GGWCC 2 cut(s) 261, 407
Eco47III AGCGCT 1 cut(s) 17
Eco57I CTGAAG 1 cut(s) 384
EcoNI CCTNNNNNAGG 1 cut(s) 657
EcoO109I RGGNCCY 1 cut(s) 261
EcoRI GAATTC 1 cut(s) 330
EcoRII CCWGG 3 cut(s) 52, 218, 652
EcoT14I CCWWGG 2 cut(s) 156, 465
ErhI CCWWGG 2 cut(s) 156, 465
FaeI CATG 8 cut(s) 154, 160, 188, 283, 469, 490, 641, 746
FaqI GGGAC 1 cut(s) 247
FatI CATG 8 cut(s) 150, 156, 184, 279, 465, 486, 637, 742
FauI CCCGC 1 cut(s) 273
FblI GTMKAC 2 cut(s) 338, 423
Fnu4HI GCNGC 3 cut(s) 249, 669, 672
Fsp4HI GCNGC 3 cut(s) 249, 669, 672
GlaI GCGC 1 cut(s) 17
GluI GCNGC 3 cut(s) 249, 669, 672
HaeII RGCGCY 1 cut(s) 19
HaeIII GGCC 3 cut(s) 438, 461, 514
HapII CCGG 1 cut(s) 677
HhaI GCGC 1 cut(s) 18
Hin1II CATG 8 cut(s) 154, 160, 188, 283, 469, 490, 641, 746
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HincII GTYRAC 1 cut(s) 339
HindII GTYRAC 1 cut(s) 339
HinfI GANTC 3 cut(s) 224, 304, 626
HpaII CCGG 1 cut(s) 677
HphI GGTGA 2 cut(s) 208, 668
Hpy166II GTNNAC 3 cut(s) 339, 424, 635
Hpy188I TCNGA 4 cut(s) 321, 403, 434, 541
Hpy188III TCNNGA 2 cut(s) 295, 308
Hpy8I GTNNAC 3 cut(s) 339, 424, 635
Hpy99I CGWCG 1 cut(s) 76
HpyAV CCTTC 4 cut(s) 75, 221, 251, 653
HpyCH4III ACNGT 1 cut(s) 724
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 3 cut(s) 150, 674, 694
HpyF10VI GCNNNNNNNGC 5 cut(s) 185, 435, 458, 520, 680
HpyF3I CTNAG 3 cut(s) 124, 180, 703
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 8 cut(s) 154, 160, 188, 283, 469, 490, 641, 746
HspAI GCGC 1 cut(s) 16
KflI GGGWCCC 1 cut(s) 261
Kzo9I GATC 3 cut(s) 57, 379, 595
LmnI GCTCC 1 cut(s) 131
Lsp1109I GCAGC 3 cut(s) 260, 658, 680
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 1 cut(s) 301
MalI GATC 3 cut(s) 59, 381, 597
MboI GATC 3 cut(s) 57, 379, 595
MboII GAAGA 6 cut(s) 25, 390, 407, 433, 528, 633
MflI RGATCY 1 cut(s) 595
MlsI TGGCCA 1 cut(s) 514
MluCI AATT 5 cut(s) 118, 330, 393, 560, 601
MluNI TGGCCA 1 cut(s) 514
MlyI GAGTC 1 cut(s) 298
MnlI CCTC 8 cut(s) 32, 133, 279, 370, 397, 428, 449, 472
Mox20I TGGCCA 1 cut(s) 514
MscI TGGCCA 1 cut(s) 514
MseI TTAA 3 cut(s) 111, 116, 615
MslI CAYNNNNRTG 2 cut(s) 155, 722
Msp20I TGGCCA 1 cut(s) 514
MspA1I CMGCKG 2 cut(s) 268, 671
MspI CCGG 1 cut(s) 677
MspR9I CCNGG 4 cut(s) 54, 220, 654, 678
MvaI CCWGG 3 cut(s) 54, 220, 654
MwoI GCNNNNNNNGC 5 cut(s) 185, 435, 458, 520, 680
NciI CCSGG 1 cut(s) 678
NcoI CCATGG 2 cut(s) 156, 465
NdeII GATC 3 cut(s) 57, 379, 595
NlaIII CATG 8 cut(s) 154, 160, 188, 283, 469, 490, 641, 746
NlaIV GGNNCC 3 cut(s) 262, 263, 408
NmuCI GTSAC 1 cut(s) 301
NspI RCATGY 4 cut(s) 188, 283, 490, 641
PaeI GCATGC 1 cut(s) 188
PaqCI CACCTGC 1 cut(s) 443
PasI CCCWGGG 1 cut(s) 53
PceI AGGCCT 1 cut(s) 438
PciI ACATGT 2 cut(s) 279, 486
PfeI GAWTC 2 cut(s) 224, 626
PkrI GCNGC 3 cut(s) 250, 670, 673
PleI GAGTC 1 cut(s) 298
PpsI GAGTC 1 cut(s) 298
PpuMI RGGWCCY 1 cut(s) 261
PscI ACATGT 2 cut(s) 279, 486
Psp5II RGGWCCY 1 cut(s) 261
Psp6I CCWGG 3 cut(s) 52, 218, 652
PspGI CCWGG 3 cut(s) 52, 218, 652
PspN4I GGNNCC 3 cut(s) 262, 263, 408
PspPI GGNCC 2 cut(s) 261, 407
PspPPI RGGWCCY 1 cut(s) 261
PsrI GAACNNNNNNTAC 2 cut(s) 90, 122
PsuI RGATCY 1 cut(s) 595
PvuII CAGCTG 1 cut(s) 671
RsaI GTAC 3 cut(s) 93, 485, 636
RsaNI GTAC 3 cut(s) 92, 484, 635
RseI CAYNNNNRTG 2 cut(s) 155, 722
SalI GTCGAC 1 cut(s) 337
SaqAI TTAA 3 cut(s) 111, 116, 615
SatI GCNGC 3 cut(s) 249, 669, 672
Sau3AI GATC 3 cut(s) 57, 379, 595
Sau96I GGNCC 2 cut(s) 261, 407
SchI GAGTC 1 cut(s) 298
ScrFI CCNGG 4 cut(s) 54, 220, 654, 678
SetI ASST 9 cut(s) 136, 303, 370, 374, 431, 457, 520, 664, 673
SfcI CTRYAG 1 cut(s) 424
SinI GGWCC 2 cut(s) 261, 407
SmiMI CAYNNNNRTG 2 cut(s) 155, 722
SmlI CTYRAG 1 cut(s) 306
SmoI CTYRAG 1 cut(s) 306
SphI GCATGC 1 cut(s) 188
Sse9I AATT 5 cut(s) 118, 330, 393, 560, 601
SseBI AGGCCT 1 cut(s) 438
SsiI CCGC 1 cut(s) 266
StuI AGGCCT 1 cut(s) 438
StyD4I CCNGG 4 cut(s) 52, 218, 652, 676
StyI CCWWGG 2 cut(s) 156, 465
TaaI ACNGT 1 cut(s) 724
TaiI ACGT 1 cut(s) 303
TaqI TCGA 3 cut(s) 60, 284, 338
TasI AATT 5 cut(s) 118, 330, 393, 560, 601
TatI WGTACW 2 cut(s) 483, 634
TfiI GAWTC 2 cut(s) 224, 626
Tru1I TTAA 3 cut(s) 111, 116, 615
Tru9I TTAA 3 cut(s) 111, 116, 615
TscAI CASTG 2 cut(s) 132, 729
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 3 cut(s) 248, 668, 671
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 3 cut(s) 19, 25, 433
TspRI CASTG 2 cut(s) 132, 729
VpaK11BI GGWCC 2 cut(s) 261, 407
XagI CCTNNNNNAGG 1 cut(s) 657
XapI RAATTY 3 cut(s) 118, 330, 393
XceI RCATGY 4 cut(s) 188, 283, 490, 641
XmiI GTMKAC 2 cut(s) 338, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.