Rh4DG115100

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000721
Physical Location & Seq
Forward (+)
18190186 .. 18193414
3229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG115100.1

Sequence Viewer

Length: 543 bp
ATGGCAAACCAGAAGCATTTCCTCTGGTTCATCTTCATTGTTTTGTTCATCCCTGGGATCGAGCCTTCAACGACAAAAGAGGTGATACGAGTACCAGTAGGAGTTGTTCTTAACTTAAATTCCTCAGTGGGAGCTATGGCAGAGAACTGCATGACCATGGCACTTGATGATTTCTATGCTAAGCATGCTCATTATCAAACAAGGCTTTTCTTTCTCACCAGGGATTCAAAGAATGATGTTGTTTCAGCAGCATTTGAAGCTTTGCGAACTGAGAGCACATTTTGGGAGAAATTGGTTGAAGTGGCAAATCATTTTGACAAGAAAGATCTCACTTCACATCAGTTTAAGCGAAGAGAATCAAGAGTACATGCTTTAGACACTCCTGATGAAGGTCTAGCAGCTGCACCTGGTGCTAATGATATGCAAAACAACTCAGCAGGAACTCACATAACAGTGGAAAACAATCTTGGACATGATGAAAATGAAAATCTTTCATCAGGGCATACGGATTCATCTATGCCTGTACTCGACTCATCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

180

Amino Acids

20.05

Weight (kDa)

5.38

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 65
AcsI RAATTY 1 cut(s) 118
AdeI CACNNNGTG 1 cut(s) 410
AfaI GTAC 3 cut(s) 93, 366, 525
AfiI CCNNNNNNNGG 1 cut(s) 389
AgsI TTSAA 4 cut(s) 69, 228, 257, 299
AjnI CCWGG 3 cut(s) 52, 218, 406
AjuI GAANNNNNNNTTGG 2 cut(s) 450, 482
AluBI AGCT 3 cut(s) 134, 260, 401
AluI AGCT 3 cut(s) 134, 260, 401
Alw21I GWGCWC 1 cut(s) 278
AlwI GGATC 1 cut(s) 65
ApeKI GCWGC 3 cut(s) 248, 398, 401
ApoI RAATTY 1 cut(s) 118
Asp700I GAANNNNTTC 1 cut(s) 17
AsuHPI GGTGA 2 cut(s) 94, 208
Bbv12I GWGCWC 1 cut(s) 278
BbvI GCAGC 3 cut(s) 260, 388, 410
BciT130I CCWGG 3 cut(s) 54, 220, 408
BfaI CTAG 1 cut(s) 395
BglII AGATCT 1 cut(s) 325
BisI GCNGC 3 cut(s) 249, 399, 402
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 3 cut(s) 250, 400, 403
Bme1390I CCNGG 3 cut(s) 54, 220, 408
BmrFI CCNGG 3 cut(s) 54, 220, 408
Bpu1102I GCTNAGC 1 cut(s) 180
BsaJI CCNNGG 4 cut(s) 52, 53, 156, 219
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 1 cut(s) 95
BseBI CCWGG 3 cut(s) 54, 220, 408
BseDI CCNNGG 4 cut(s) 52, 53, 156, 219
BseGI GGATG 1 cut(s) 48
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMII CTCAG 3 cut(s) 138, 261, 447
BseNI ACTGG 1 cut(s) 95
BseXI GCAGC 3 cut(s) 260, 388, 410
BsgI GTGCAG 1 cut(s) 387
BsiHKAI GWGCWC 1 cut(s) 278
BslI CCNNNNNNNGG 1 cut(s) 389
Bsp1286I GDGCHC 1 cut(s) 278
Bsp143I GATC 2 cut(s) 57, 325
Bsp1720I GCTNAGC 1 cut(s) 180
Bsp19I CCATGG 1 cut(s) 156
BspCNI CTCAG 3 cut(s) 137, 262, 446
BspPI GGATC 1 cut(s) 65
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 4 cut(s) 52, 53, 156, 219
BssMI GATC 2 cut(s) 57, 325
BssT1I CCWWGG 1 cut(s) 156
Bst2UI CCWGG 3 cut(s) 54, 220, 408
Bst4CI ACNGT 1 cut(s) 454
Bst6I CTCTTC 1 cut(s) 346
BstAPI GCANNNNNTGC 1 cut(s) 410
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 4 cut(s) 124, 180, 270, 433
BstDSI CCRYGG 1 cut(s) 156
BstENI CCTNNNNNAGG 1 cut(s) 387
BstF5I GGATG 1 cut(s) 48
BstKTI GATC 2 cut(s) 60, 328
BstMBI GATC 2 cut(s) 57, 325
BstMWI GCNNNNNNNGC 3 cut(s) 185, 257, 410
BstNI CCWGG 3 cut(s) 54, 220, 408
BstNSI RCATGY 2 cut(s) 188, 371
BstSCI CCNGG 3 cut(s) 52, 218, 406
BstV1I GCAGC 3 cut(s) 260, 388, 410
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 1 cut(s) 48
BtsIMutI CAGTG 2 cut(s) 132, 459
Cac8I GCNNGC 1 cut(s) 186
CsiI ACCWGGT 1 cut(s) 406
Csp6I GTAC 3 cut(s) 92, 365, 524
CviAII CATG 5 cut(s) 151, 157, 185, 368, 473
CviJI RGCY 5 cut(s) 64, 134, 205, 260, 401
CviKI_1 RGCY 5 cut(s) 64, 134, 205, 260, 401
CviQI GTAC 3 cut(s) 92, 365, 524
DdeI CTNAG 4 cut(s) 124, 180, 270, 433
DpnI GATC 2 cut(s) 59, 327
DpnII GATC 2 cut(s) 57, 325
DraIII CACNNNGTG 1 cut(s) 410
Eam1104I CTCTTC 1 cut(s) 346
EarI CTCTTC 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 156
EcoNI CCTNNNNNAGG 1 cut(s) 387
EcoRII CCWGG 3 cut(s) 52, 218, 406
EcoT14I CCWWGG 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 5 cut(s) 154, 160, 188, 371, 476
FatI CATG 5 cut(s) 150, 156, 184, 367, 472
Fnu4HI GCNGC 3 cut(s) 249, 399, 402
FokI GGATG 1 cut(s) 35
Fsp4HI GCNGC 3 cut(s) 249, 399, 402
FspBI CTAG 1 cut(s) 395
GluI GCNGC 3 cut(s) 249, 399, 402
Hin1II CATG 5 cut(s) 154, 160, 188, 371, 476
HindIII AAGCTT 1 cut(s) 258
HinfI GANTC 4 cut(s) 224, 356, 509, 530
HphI GGTGA 2 cut(s) 94, 208
Hpy188III TCNNGA 2 cut(s) 360, 383
HpyAV CCTTC 2 cut(s) 75, 383
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4V TGCA 3 cut(s) 150, 404, 424
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 257, 410
HpyF3I CTNAG 4 cut(s) 124, 180, 270, 433
Hsp92II CATG 5 cut(s) 154, 160, 188, 371, 476
Kzo9I GATC 2 cut(s) 57, 325
LmnI GCTCC 1 cut(s) 131
Lsp1109I GCAGC 3 cut(s) 260, 388, 410
MabI ACCWGGT 1 cut(s) 406
MaeI CTAG 1 cut(s) 395
MalI GATC 2 cut(s) 59, 327
MboI GATC 2 cut(s) 57, 325
MboII GAAGA 2 cut(s) 25, 363
MflI RGATCY 1 cut(s) 325
MhlI GDGCHC 1 cut(s) 278
MluCI AATT 2 cut(s) 118, 290
MlyI GAGTC 1 cut(s) 524
MnlI CCTC 3 cut(s) 32, 73, 133
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 3 cut(s) 111, 116, 345
MslI CAYNNNNRTG 2 cut(s) 155, 452
MspA1I CMGCKG 1 cut(s) 401
MspR9I CCNGG 3 cut(s) 54, 220, 408
MvaI CCWGG 3 cut(s) 54, 220, 408
MwoI GCNNNNNNNGC 3 cut(s) 185, 257, 410
NcoI CCATGG 1 cut(s) 156
NdeII GATC 2 cut(s) 57, 325
NlaIII CATG 5 cut(s) 154, 160, 188, 371, 476
NspI RCATGY 2 cut(s) 188, 371
PaeI GCATGC 1 cut(s) 188
PasI CCCWGGG 1 cut(s) 53
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 3 cut(s) 224, 356, 509
PkrI GCNGC 3 cut(s) 250, 400, 403
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
Psp6I CCWGG 3 cut(s) 52, 218, 406
PspGI CCWGG 3 cut(s) 52, 218, 406
PsrI GAACNNNNNNTAC 2 cut(s) 90, 122
PsuI RGATCY 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 401
RsaI GTAC 3 cut(s) 93, 366, 525
RsaNI GTAC 3 cut(s) 92, 365, 524
RseI CAYNNNNRTG 2 cut(s) 155, 452
SaqAI TTAA 3 cut(s) 111, 116, 345
SatI GCNGC 3 cut(s) 249, 399, 402
Sau3AI GATC 2 cut(s) 57, 325
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 3 cut(s) 54, 220, 408
SduI GDGCHC 1 cut(s) 278
SetI ASST 6 cut(s) 84, 136, 262, 394, 403, 409
SexAI ACCWGGT 1 cut(s) 406
SmiMI CAYNNNNRTG 2 cut(s) 155, 452
SphI GCATGC 1 cut(s) 188
Sse9I AATT 2 cut(s) 118, 290
SspMI CTAG 1 cut(s) 395
StyD4I CCNGG 3 cut(s) 52, 218, 406
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 1 cut(s) 454
TaqI TCGA 2 cut(s) 60, 528
TasI AATT 2 cut(s) 118, 290
TatI WGTACW 2 cut(s) 364, 523
TfiI GAWTC 3 cut(s) 224, 356, 509
Tru1I TTAA 3 cut(s) 111, 116, 345
Tru9I TTAA 3 cut(s) 111, 116, 345
TscAI CASTG 2 cut(s) 132, 459
TseI GCWGC 3 cut(s) 248, 398, 401
TspDTI ATGAA 8 cut(s) 19, 25, 37, 402, 483, 492, 498, 501
TspGWI ACGGA 1 cut(s) 521
TspRI CASTG 2 cut(s) 132, 459
XagI CCTNNNNNAGG 1 cut(s) 387
XapI RAATTY 1 cut(s) 118
XceI RCATGY 2 cut(s) 188, 371
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.