Rh4AG122300

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000721
Physical Location & Seq
Forward (+)
26490736 .. 26493899
3164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG122300.1

Sequence Viewer

Length: 492 bp
ATGGCAAACCAGAAGCACTTCCTCTGGTTCATCTTCATTGTTTTGTTCATCCCTGCGATCGCGCCTTCAACGACGAAAGAGGTGATACGAGTACCAGTAGGAGTTGTTCTTAACTTAAATTCCTCAGTTGGAGCTATGGCAGAGAACTGCATGACCATGGCACTTGATGATTTTTATGCTAAGCATGCTCATTATCAAACAAGGCTTTTCTTTCTCACCAGGGATTCAAAGAATGATGTTGTTTCAGCAGCATTTGAAGCTTTGCGAACAATTAGTTCTTCTGAGAGCAGATTTTGGGAGAAATTGGTTGAAGTGGCAAAGCATTTTGACAAGAAAGATCTCACTTCACATCAGTTTAAGCGAAGAGAATCAAGAGTACATGCTTTAGACACTCCTGATGAAGGTCTAGCAGCTGCACCGGGTGCTAATGATATGCAAAACAACTCATGCAGCAGGAACTCACATAATAGTGGAGAACAATCTTGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

163

Amino Acids

18.39

Weight (kDa)

7.06

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 62
AcsI RAATTY 1 cut(s) 118
AdeI CACNNNGTG 1 cut(s) 422
AfaI GTAC 2 cut(s) 93, 378
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 4 cut(s) 69, 228, 257, 311
AjnI CCWGG 1 cut(s) 218
AluBI AGCT 3 cut(s) 134, 260, 413
AluI AGCT 3 cut(s) 134, 260, 413
ApeKI GCWGC 4 cut(s) 248, 410, 413, 450
ApoI RAATTY 1 cut(s) 118
AsiSI GCGATCGC 1 cut(s) 60
Asp700I GAANNNNTTC 1 cut(s) 17
AspLEI GCGC 1 cut(s) 64
AsuC2I CCSGG 1 cut(s) 420
AsuHPI GGTGA 2 cut(s) 94, 208
BbvI GCAGC 4 cut(s) 260, 400, 422, 462
BciT130I CCWGG 1 cut(s) 220
BcnI CCSGG 1 cut(s) 420
BfaI CTAG 1 cut(s) 407
BglII AGATCT 1 cut(s) 337
BisI GCNGC 4 cut(s) 249, 411, 414, 451
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 4 cut(s) 250, 412, 415, 452
Bme1390I CCNGG 2 cut(s) 220, 420
BmrFI CCNGG 2 cut(s) 220, 420
Bpu1102I GCTNAGC 1 cut(s) 180
BpuMI CCSGG 1 cut(s) 420
BsaJI CCNNGG 2 cut(s) 156, 219
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse1I ACTGG 1 cut(s) 95
BseBI CCWGG 1 cut(s) 220
BseDI CCNNGG 2 cut(s) 156, 219
BseGI GGATG 1 cut(s) 48
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMII CTCAG 2 cut(s) 138, 273
BseNI ACTGG 1 cut(s) 95
BseXI GCAGC 4 cut(s) 260, 400, 422, 462
BsgI GTGCAG 1 cut(s) 399
Bsh1236I CGCG 1 cut(s) 62
Bsh1285I CGRYCG 1 cut(s) 60
BsiEI CGRYCG 1 cut(s) 60
BsiSI CCGG 1 cut(s) 419
BslI CCNNNNNNNGG 1 cut(s) 401
Bsp143I GATC 2 cut(s) 57, 337
Bsp1720I GCTNAGC 1 cut(s) 180
Bsp19I CCATGG 1 cut(s) 156
BspCNI CTCAG 2 cut(s) 137, 274
BspFNI CGCG 1 cut(s) 62
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 2 cut(s) 156, 219
BssMI GATC 2 cut(s) 57, 337
BssT1I CCWWGG 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 220
Bst6I CTCTTC 1 cut(s) 358
BstAPI GCANNNNNTGC 1 cut(s) 422
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 3 cut(s) 124, 180, 282
BstDSI CCRYGG 1 cut(s) 156
BstENI CCTNNNNNAGG 1 cut(s) 399
BstF5I GGATG 1 cut(s) 48
BstFNI CGCG 1 cut(s) 62
BstHHI GCGC 1 cut(s) 64
BstKTI GATC 2 cut(s) 60, 340
BstMBI GATC 2 cut(s) 57, 337
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 3 cut(s) 185, 257, 422
BstNI CCWGG 1 cut(s) 220
BstNSI RCATGY 2 cut(s) 188, 383
BstSCI CCNGG 2 cut(s) 218, 418
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 4 cut(s) 260, 400, 422, 462
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 186
CfoI GCGC 1 cut(s) 64
Csp6I GTAC 2 cut(s) 92, 377
CviAII CATG 6 cut(s) 151, 157, 185, 380, 447, 489
CviJI RGCY 4 cut(s) 134, 205, 260, 413
CviKI_1 RGCY 4 cut(s) 134, 205, 260, 413
CviQI GTAC 2 cut(s) 92, 377
DdeI CTNAG 3 cut(s) 124, 180, 282
DpnI GATC 2 cut(s) 59, 339
DpnII GATC 2 cut(s) 57, 337
DraIII CACNNNGTG 1 cut(s) 422
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
Eco130I CCWWGG 1 cut(s) 156
EcoNI CCTNNNNNAGG 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 218
EcoT14I CCWWGG 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 6 cut(s) 154, 160, 188, 383, 450, 492
FatI CATG 6 cut(s) 150, 156, 184, 379, 446, 488
Fnu4HI GCNGC 4 cut(s) 249, 411, 414, 451
FokI GGATG 1 cut(s) 35
Fsp4HI GCNGC 4 cut(s) 249, 411, 414, 451
FspBI CTAG 1 cut(s) 407
GlaI GCGC 1 cut(s) 63
GluI GCNGC 4 cut(s) 249, 411, 414, 451
HapII CCGG 1 cut(s) 419
HhaI GCGC 1 cut(s) 64
Hin1II CATG 6 cut(s) 154, 160, 188, 383, 450, 492
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HindIII AAGCTT 1 cut(s) 258
HinfI GANTC 2 cut(s) 224, 368
HpaII CCGG 1 cut(s) 419
HphI GGTGA 2 cut(s) 94, 208
Hpy188I TCNGA 1 cut(s) 283
Hpy188III TCNNGA 2 cut(s) 372, 395
Hpy99I CGWCG 1 cut(s) 76
HpyAV CCTTC 2 cut(s) 75, 395
HpyCH4V TGCA 4 cut(s) 150, 416, 436, 450
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 257, 422
HpyF3I CTNAG 3 cut(s) 124, 180, 282
Hsp92II CATG 6 cut(s) 154, 160, 188, 383, 450, 492
HspAI GCGC 1 cut(s) 62
Kzo9I GATC 2 cut(s) 57, 337
LmnI GCTCC 1 cut(s) 131
LpnPI CCDG 9 cut(s) 10, 23, 66, 108, 205, 232, 408, 432, 439
Lsp1109I GCAGC 4 cut(s) 260, 400, 422, 462
MaeI CTAG 1 cut(s) 407
MalI GATC 2 cut(s) 59, 339
MboI GATC 2 cut(s) 57, 337
MboII GAAGA 3 cut(s) 25, 270, 375
MflI RGATCY 1 cut(s) 337
MluCI AATT 3 cut(s) 118, 270, 302
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 3 cut(s) 32, 73, 133
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 3 cut(s) 111, 116, 357
MslI CAYNNNNRTG 2 cut(s) 155, 468
MspA1I CMGCKG 1 cut(s) 413
MspI CCGG 1 cut(s) 419
MspR9I CCNGG 2 cut(s) 220, 420
MvaI CCWGG 1 cut(s) 220
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 3 cut(s) 185, 257, 422
NciI CCSGG 1 cut(s) 420
NcoI CCATGG 1 cut(s) 156
NdeII GATC 2 cut(s) 57, 337
NlaIII CATG 6 cut(s) 154, 160, 188, 383, 450, 492
NspI RCATGY 2 cut(s) 188, 383
PaeI GCATGC 1 cut(s) 188
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 2 cut(s) 224, 368
PkrI GCNGC 4 cut(s) 250, 412, 415, 452
Ple19I CGATCG 1 cut(s) 60
Psp6I CCWGG 1 cut(s) 218
PspGI CCWGG 1 cut(s) 218
PsrI GAACNNNNNNTAC 2 cut(s) 90, 122
PsuI RGATCY 1 cut(s) 337
PvuI CGATCG 1 cut(s) 60
PvuII CAGCTG 1 cut(s) 413
RgaI GCGATCGC 1 cut(s) 60
RsaI GTAC 2 cut(s) 93, 378
RsaNI GTAC 2 cut(s) 92, 377
RseI CAYNNNNRTG 2 cut(s) 155, 468
SaqAI TTAA 3 cut(s) 111, 116, 357
SatI GCNGC 4 cut(s) 249, 411, 414, 451
Sau3AI GATC 2 cut(s) 57, 337
ScrFI CCNGG 2 cut(s) 220, 420
SetI ASST 5 cut(s) 84, 136, 262, 406, 415
SfaAI GCGATCGC 1 cut(s) 60
SgfI GCGATCGC 1 cut(s) 60
SmiMI CAYNNNNRTG 2 cut(s) 155, 468
SphI GCATGC 1 cut(s) 188
Sse9I AATT 3 cut(s) 118, 270, 302
SspMI CTAG 1 cut(s) 407
StyD4I CCNGG 2 cut(s) 218, 418
StyI CCWWGG 1 cut(s) 156
TasI AATT 3 cut(s) 118, 270, 302
TatI WGTACW 1 cut(s) 376
TfiI GAWTC 2 cut(s) 224, 368
Tru1I TTAA 3 cut(s) 111, 116, 357
Tru9I TTAA 3 cut(s) 111, 116, 357
TseI GCWGC 4 cut(s) 248, 410, 413, 450
TspDTI ATGAA 4 cut(s) 19, 25, 37, 414
XagI CCTNNNNNAGG 1 cut(s) 399
XapI RAATTY 1 cut(s) 118
XceI RCATGY 2 cut(s) 188, 383
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.