Rroxscaffold_2G00126200

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000467
Physical Location & Seq
Reverse (-)
61277340 .. 61293014
15675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00126200.1

Sequence Viewer

Length: 1254 bp
ATGGGAAACATCTTTGCCAACTGTTTTACCTGTAAACAAAAGAGTGGTTCTTCAGCTGACAGAATCAGTGATCTCCCAGATGATATATTGCACGTCATACTCTCATTCTTACCTTTCAAATCCGTTGGGCAAACAGCTATCTTATCTCGAAGATGGAATGATGTATGGTCTTCCTATCCCATCATCGACTTCTACGAGATTTTTACTGGTAGTGAAGCTGATCATCACCAGACAAAAACACGGATCATCAACACAATATTAGCTCGCCATGAAGAAAACTATAATATAATGTACCCATGCCGCAAAATTTCCTCGCCAAGAGTTGCGAGGGATCCGAACAAATCTTGTTTGTCTAGGGTTGAGGTACTTGTACTCGACATCCGGTTGAGTTCTGGAAACACATCTGATTTGCCTCAATGTCAACTTATGTGCCACTCATTAAGAAGTAGTAGCTGTGGGAGTTCAAAGGAAAGGCTAGGGTTTCCCTCTTCTTATGTTGTCGAGTCTTATCTCCGTTCACTTCATACTTTGTCTCTAACTCGTGTGGATTTCTTGGATGGTAGTGCTTTGGGTGTGGATTTATTTTCTGGTTCTTCATTCCCTTATCTTGAAAAGTTGAACTTAGAGCGCTGTACAGGAAGGAATTTTGAGGAGTTTGGCATTACTGAGAGGTTTTCGATCCAGAGCTTTCCTATGCTCAAAAGATCTTTCATAAGTTCCTCCCTTACAACGAAGAAGATAAATAATAACAGTGTTGTCGATCTTCTTTCCTCTTCATCCCAAGTTGAATACTTCGAAATATCCCATGACTACCTCCAGATTCTATCAGAAATATATTTTGAATTAGGCGGTATACCGTTCTCATTTATGAAACTGAAAACCTTGAGAATTAGGAGTCCAATAAAGCAAAGATATATCCCGGGTATGGCATGCCTGTTCAAGAGCTCTCCCGTGGTCCACGACCTCGAGATTTGTTTTTGCTCTTACGAAGAAAATGGCAAGTGGAATAACCAATCCTTGGATGATGCCAATTGCACCCAAGAACAATACTGGGAGACTCAGGGTCAATATTTGAGCCCCTCTCTTAGTCACCTAAAGGTGGTCAGAATTCGCCTTGGTCAAATGATCCCCGAGAGTGCAATCAAATTTGCAAAGTTTGTGCTCAAATATGGAAGAAACCTGCAAAAAATGAGTCTGGATGAAGTGGACTTGGGAATGACATTTGAGGAATTCTCAGTCTGGAAGGTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

417

Amino Acids

47.6

Weight (kDa)

7.52

Isoelectric Point (pI)

55.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 60 1.4e-09 F-box domain
F-box-like PF12937 22 - 55 5.9e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000467)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g02550 FvH4_6g26921 FvH4_6g45630
malus_domestica MD01G1113200.v1.1 MD09G1101600.v1.1 MD17G1084600.v1.1
prunus_persica Prupe.3G224400_v2.0.a1 Prupe.3G224500_v2.0.a1 Prupe.3G224600_v2.0.a1
pyrus_communis pycom02g19930 pycom09g00760 pycom09g00770 pycom17g08170
rosa_chinensis RchiOBHm_Chr1g0350511 RchiOBHm_Chr2g0086171 RchiOBHm_Chr2g0117831 RchiOBHm_Chr3g0450391 RchiOBHm_Chr4g0409631 RchiOBHm_Chr5g0039731
rosa_laevigata RLG00000008538 RLG00000018375 RLG00000021415 RLG00000028481 RLG00000033926 RLG00000033937 RLG00000033938 RLG00000033939 RLG00000033941
rosa_multiflora Rmu_sc0000103.1_g000011 Rmu_sc0000330.1_g000009 Rmu_sc0000652.1_g000002 Rmu_sc0001507.1_g000017 Rmu_sc0004020.1_g000010 Rmu_sc0026676.1_g000001 Rmu_ssc0000083.1_g000022 Rmu_ssc0000400.1_g000021
rosa_roxburghii Rroxscaffold_1G00040760 Rroxscaffold_1G00041490 Rroxscaffold_1G00041500 Rroxscaffold_1G00041630 Rroxscaffold_2G00087990 Rroxscaffold_2G00126200 Rroxscaffold_4G00304800 Rroxscaffold_5G00354160 Rroxscaffold_5G00354170
rosa_rugosa Rorug01G0211500 Rorug02G0214300 Rorug02G0496800 Rorug02G0627700 Rorug04G0095600 Rorug05G0181700
rosa_samantha Rh1AG226300 Rh1BG194700 Rh1CG211100 Rh1DG222900 Rh2AG270500 Rh2AG270600 Rh2AG562900 Rh2AG563000 Rh2BG281900 Rh2BG282000 Rh2BG575700 Rh2CG269300 Rh2CG269400 Rh2DG278300 Rh2DG296200 Rh2DG585900 Rh3AG028000 Rh3AG028100 Rh3BG028500 Rh3BG028600 Rh3CG027300 Rh3CG027400 Rh3DG028400 Rh3DG028500 Rh4BG156800 Rh4CG167300 Rh4CG167400 Rh4DG151100 Rh5AG268000 Rh5BG272400 Rh5BG272500 Rh5CG304000 Rh5CG305000 Rh5CG305100 Rh5DG280500
rosa_wichuraiana Rw1G019530 Rw2G021370 Rw4G012860 Rw4G012970 Rw5G025070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1188
AccB7I CCANNNNNTGG 1 cut(s) 1018
AccI GTMKAC 1 cut(s) 853
AciI CCGC 2 cut(s) 301, 849
AclWI GGATC 5 cut(s) 251, 326, 339, 673, 1120
AcsI RAATTY 5 cut(s) 306, 643, 1107, 1145, 1229
AcuI CTGAAG 1 cut(s) 36
AfaI GTAC 4 cut(s) 293, 366, 372, 634
AfeI AGCGCT 1 cut(s) 629
AfiI CCNNNNNNNGG 3 cut(s) 925, 1018, 1099
AgsI TTSAA 7 cut(s) 118, 465, 611, 619, 788, 842, 940
AhdI GACNNNNNGTC 1 cut(s) 1062
AjiI CACGTC 1 cut(s) 94
AluBI AGCT 7 cut(s) 56, 137, 218, 263, 453, 687, 945
AluI AGCT 7 cut(s) 56, 137, 218, 263, 453, 687, 945
Alw21I GWGCWC 2 cut(s) 947, 1164
Alw26I GTCTC 2 cut(s) 537, 1049
AlwI GGATC 5 cut(s) 251, 326, 339, 673, 1120
Ama87I CYCGRG 3 cut(s) 919, 965, 1130
Aor51HI AGCGCT 1 cut(s) 629
ApoI RAATTY 5 cut(s) 306, 643, 1107, 1145, 1229
ArsI GACNNNNNNTTYG 2 cut(s) 1177, 1209
AspLEI GCGC 1 cut(s) 630
AspS9I GGNCC 1 cut(s) 955
AsuC2I CCSGG 2 cut(s) 920, 921
AsuHPI GGTGA 2 cut(s) 218, 1082
AsuII TTCGAA 1 cut(s) 795
AvaI CYCGRG 3 cut(s) 919, 965, 1130
AvaII GGWCC 1 cut(s) 955
BamHI GGATCC 1 cut(s) 331
BanII GRGCYC 2 cut(s) 947, 1079
BauI CACGAG 1 cut(s) 540
BbsI GAAGAC 1 cut(s) 162
Bbv12I GWGCWC 2 cut(s) 947, 1164
BccI CCATC 3 cut(s) 147, 188, 551
BclI TGATCA 1 cut(s) 220
BcnI CCSGG 2 cut(s) 920, 921
BcoDI GTCTC 2 cut(s) 537, 1049
BfaI CTAG 2 cut(s) 354, 476
BfoI RGCGCY 1 cut(s) 631
BfuAI ACCTGC 1 cut(s) 1188
BglII AGATCT 1 cut(s) 704
BisI GCNGC 1 cut(s) 301
BlsI GCNGC 1 cut(s) 302
Bme1390I CCNGG 2 cut(s) 920, 921
Bme18I GGWCC 1 cut(s) 955
BmeRI GACNNNNNGTC 1 cut(s) 1062
BmeT110I CYCGRG 3 cut(s) 919, 965, 1130
BmgBI CACGTC 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 955
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 2 cut(s) 920, 921
BmrI ACTGGG 1 cut(s) 1060
BmsI GCATC 1 cut(s) 1015
BmuI ACTGGG 1 cut(s) 1060
BpiI GAAGAC 1 cut(s) 162
BplI GAGNNNNNCTC 4 cut(s) 1066, 1098, 1217, 1249
BpmI CTGGAG 1 cut(s) 800
Bpu14I TTCGAA 1 cut(s) 795
BpuEI CTTGAG 1 cut(s) 904
BpuMI CCSGG 2 cut(s) 920, 921
BsaJI CCNNGG 4 cut(s) 919, 951, 1017, 1114
BsaWI WCCGGW 1 cut(s) 381
Bsc4I CCNNNNNNNGG 3 cut(s) 925, 1018, 1099
Bse1I ACTGG 2 cut(s) 211, 1055
BseDI CCNNGG 4 cut(s) 919, 951, 1017, 1114
BseGI GGATG 5 cut(s) 378, 562, 776, 1027, 1204
BseLI CCNNNNNNNGG 3 cut(s) 925, 1018, 1099
BseMII CTCAG 3 cut(s) 657, 1073, 1248
BseNI ACTGG 2 cut(s) 211, 1055
BseRI GAGGAG 1 cut(s) 665
BsiHKAI GWGCWC 2 cut(s) 947, 1164
BsiHKCI CYCGRG 3 cut(s) 919, 965, 1130
BsiSI CCGG 2 cut(s) 382, 920
BslI CCNNNNNNNGG 3 cut(s) 925, 1018, 1099
BsmAI GTCTC 2 cut(s) 537, 1049
BsoBI CYCGRG 3 cut(s) 919, 965, 1130
Bsp119I TTCGAA 1 cut(s) 795
Bsp1286I GDGCHC 3 cut(s) 947, 1079, 1164
Bsp1407I TGTACA 1 cut(s) 632
Bsp143I GATC 8 cut(s) 70, 220, 243, 331, 678, 704, 760, 1125
BspACI CCGC 2 cut(s) 301, 849
BspCNI CTCAG 3 cut(s) 658, 1072, 1247
BspLI GGNNCC 1 cut(s) 333
BspMI ACCTGC 1 cut(s) 1188
BspPI GGATC 5 cut(s) 251, 326, 339, 673, 1120
BspT104I TTCGAA 1 cut(s) 795
BsrGI TGTACA 1 cut(s) 632
BsrI ACTGG 2 cut(s) 211, 1055
BssECI CCNNGG 4 cut(s) 919, 951, 1017, 1114
BssMI GATC 8 cut(s) 70, 220, 243, 331, 678, 704, 760, 1125
BssNAI GTATAC 1 cut(s) 854
BssSI CACGAG 1 cut(s) 540
BssT1I CCWWGG 2 cut(s) 1017, 1114
Bst1107I GTATAC 1 cut(s) 854
Bst2BI CACGAG 1 cut(s) 540
Bst4CI ACNGT 3 cut(s) 23, 752, 858
Bst6I CTCTTC 2 cut(s) 493, 778
BstAUI TGTACA 1 cut(s) 632
BstBI TTCGAA 1 cut(s) 795
BstC8I GCNNGC 2 cut(s) 265, 931
BstDEI CTNAG 5 cut(s) 622, 666, 1059, 1085, 1234
BstDSI CCRYGG 1 cut(s) 951
BstF5I GGATG 5 cut(s) 378, 562, 776, 1027, 1204
BstH2I RGCGCY 1 cut(s) 631
BstHHI GCGC 1 cut(s) 630
BstKTI GATC 8 cut(s) 73, 223, 246, 334, 681, 707, 763, 1128
BstMAI GTCTC 2 cut(s) 537, 1049
BstMBI GATC 8 cut(s) 70, 220, 243, 331, 678, 704, 760, 1125
BstNSI RCATGY 1 cut(s) 933
BstSCI CCNGG 2 cut(s) 918, 919
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 2 cut(s) 331, 704
BstYI RGATCY 2 cut(s) 331, 704
BstZ17I GTATAC 1 cut(s) 854
BtgI CCRYGG 1 cut(s) 951
BtrI CACGTC 1 cut(s) 94
BtsCI GGATG 5 cut(s) 378, 562, 776, 1027, 1204
BtsIMutI CAGTG 2 cut(s) 73, 757
BveI ACCTGC 1 cut(s) 1188
Cac8I GCNNGC 2 cut(s) 265, 931
CfoI GCGC 1 cut(s) 630
Cfr13I GGNCC 1 cut(s) 955
Cfr9I CCCGGG 1 cut(s) 919
Csp6I GTAC 4 cut(s) 292, 365, 371, 633
CviAII CATG 4 cut(s) 269, 297, 806, 930
CviJI RGCY 9 cut(s) 56, 137, 218, 263, 453, 475, 687, 945, 1077
CviKI_1 RGCY 9 cut(s) 56, 137, 218, 263, 453, 475, 687, 945, 1077
CviQI GTAC 4 cut(s) 292, 365, 371, 633
DdeI CTNAG 5 cut(s) 622, 666, 1059, 1085, 1234
DpnI GATC 8 cut(s) 72, 222, 245, 333, 680, 706, 762, 1127
DpnII GATC 8 cut(s) 70, 220, 243, 331, 678, 704, 760, 1125
DriI GACNNNNNGTC 1 cut(s) 1062
Eam1104I CTCTTC 2 cut(s) 493, 778
Eam1105I GACNNNNNGTC 1 cut(s) 1062
EarI CTCTTC 2 cut(s) 493, 778
Ecl136II GAGCTC 1 cut(s) 945
Eco130I CCWWGG 2 cut(s) 1017, 1114
Eco24I GRGCYC 2 cut(s) 947, 1079
Eco47I GGWCC 1 cut(s) 955
Eco47III AGCGCT 1 cut(s) 629
Eco53kI GAGCTC 1 cut(s) 945
Eco57I CTGAAG 1 cut(s) 36
Eco88I CYCGRG 3 cut(s) 919, 965, 1130
EcoICRI GAGCTC 1 cut(s) 945
EcoRI GAATTC 2 cut(s) 1107, 1229
EcoT14I CCWWGG 2 cut(s) 1017, 1114
EcoT38I GRGCYC 2 cut(s) 947, 1079
ErhI CCWWGG 2 cut(s) 1017, 1114
FaeI CATG 4 cut(s) 272, 300, 809, 933
FalI AAGNNNNNCTT 2 cut(s) 605, 637
FatI CATG 4 cut(s) 268, 296, 805, 929
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 1 cut(s) 853
Fnu4HI GCNGC 1 cut(s) 301
FokI GGATG 5 cut(s) 365, 569, 763, 1034, 1211
FriOI GRGCYC 2 cut(s) 947, 1079
Fsp4HI GCNGC 1 cut(s) 301
FspBI CTAG 2 cut(s) 354, 476
GlaI GCGC 1 cut(s) 629
GluI GCNGC 1 cut(s) 301
GsuI CTGGAG 1 cut(s) 800
HaeII RGCGCY 1 cut(s) 631
HapII CCGG 2 cut(s) 382, 920
HhaI GCGC 1 cut(s) 630
Hin1II CATG 4 cut(s) 272, 300, 809, 933
Hin6I GCGC 1 cut(s) 628
HinP1I GCGC 1 cut(s) 628
HincII GTYRAC 1 cut(s) 422
HindII GTYRAC 1 cut(s) 422
HinfI GANTC 6 cut(s) 63, 503, 820, 895, 1057, 1192
HpaII CCGG 2 cut(s) 382, 920
HphI GGTGA 2 cut(s) 218, 1082
Hpy166II GTNNAC 6 cut(s) 35, 422, 518, 854, 958, 1207
Hpy188I TCNGA 4 cut(s) 336, 406, 829, 1106
Hpy188III TCNNGA 9 cut(s) 147, 393, 608, 682, 817, 940, 967, 1196, 1240
Hpy8I GTNNAC 6 cut(s) 35, 422, 518, 854, 958, 1207
HpyAV CCTTC 2 cut(s) 633, 1237
HpyCH4III ACNGT 3 cut(s) 23, 752, 858
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 6 cut(s) 91, 1035, 1139, 1151, 1183, 1250
HpyF3I CTNAG 5 cut(s) 622, 666, 1059, 1085, 1234
HpySE526I ACGT 1 cut(s) 93
Hsp92II CATG 4 cut(s) 272, 300, 809, 933
HspAI GCGC 1 cut(s) 628
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 8 cut(s) 70, 220, 243, 331, 678, 704, 760, 1125
LweI GCATC 1 cut(s) 1015
MaeI CTAG 2 cut(s) 354, 476
MaeII ACGT 1 cut(s) 93
MaeIII GTNAC 1 cut(s) 1088
MalI GATC 8 cut(s) 72, 222, 245, 333, 680, 706, 762, 1127
MboI GATC 8 cut(s) 70, 220, 243, 331, 678, 704, 760, 1125
MfeI CAATTG 1 cut(s) 1030
MflI RGATCY 2 cut(s) 331, 704
MhlI GDGCHC 3 cut(s) 947, 1079, 1164
MluCI AATT 8 cut(s) 306, 643, 842, 888, 1030, 1107, 1145, 1229
MlyI GAGTC 4 cut(s) 512, 904, 1051, 1201
MseI TTAA 1 cut(s) 440
MspA1I CMGCKG 1 cut(s) 56
MspI CCGG 2 cut(s) 382, 920
MspR9I CCNGG 2 cut(s) 920, 921
MunI CAATTG 1 cut(s) 1030
NciI CCSGG 2 cut(s) 920, 921
NdeII GATC 8 cut(s) 70, 220, 243, 331, 678, 704, 760, 1125
NlaIII CATG 4 cut(s) 272, 300, 809, 933
NlaIV GGNNCC 1 cut(s) 333
NmuCI GTSAC 1 cut(s) 1088
NspI RCATGY 1 cut(s) 933
NspV TTCGAA 1 cut(s) 795
PaeI GCATGC 1 cut(s) 933
PaeR7I CTCGAG 1 cut(s) 965
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PfeI GAWTC 2 cut(s) 63, 820
PflMI CCANNNNNTGG 1 cut(s) 1018
PkrI GCNGC 1 cut(s) 302
PleI GAGTC 4 cut(s) 511, 903, 1051, 1200
PpsI GAGTC 4 cut(s) 511, 903, 1051, 1200
Psp124BI GAGCTC 1 cut(s) 947
PspN4I GGNNCC 1 cut(s) 333
PspPI GGNCC 1 cut(s) 955
PsuI RGATCY 2 cut(s) 331, 704
PvuII CAGCTG 1 cut(s) 56
RsaI GTAC 4 cut(s) 293, 366, 372, 634
RsaNI GTAC 4 cut(s) 292, 365, 371, 633
SacI GAGCTC 1 cut(s) 947
SaqAI TTAA 1 cut(s) 440
SatI GCNGC 1 cut(s) 301
Sau3AI GATC 8 cut(s) 70, 220, 243, 331, 678, 704, 760, 1125
Sau96I GGNCC 1 cut(s) 955
SchI GAGTC 4 cut(s) 512, 904, 1051, 1201
ScrFI CCNGG 2 cut(s) 920, 921
SduI GDGCHC 3 cut(s) 947, 1079, 1164
SfaNI GCATC 1 cut(s) 1015
Sfr274I CTCGAG 1 cut(s) 965
SfuI TTCGAA 1 cut(s) 795
SinI GGWCC 1 cut(s) 955
SlaI CTCGAG 1 cut(s) 965
SmaI CCCGGG 1 cut(s) 921
SmlI CTYRAG 2 cut(s) 883, 965
SmoI CTYRAG 2 cut(s) 883, 965
SphI GCATGC 1 cut(s) 933
Sse9I AATT 8 cut(s) 306, 643, 842, 888, 1030, 1107, 1145, 1229
SsiI CCGC 2 cut(s) 301, 849
SspI AATATT 2 cut(s) 258, 1070
SspMI CTAG 2 cut(s) 354, 476
SstI GAGCTC 1 cut(s) 947
StyD4I CCNGG 2 cut(s) 918, 919
StyI CCWWGG 2 cut(s) 1017, 1114
TaaI ACNGT 3 cut(s) 23, 752, 858
TaiI ACGT 1 cut(s) 96
TaqI TCGA 8 cut(s) 148, 186, 375, 501, 677, 759, 795, 966
TasI AATT 8 cut(s) 306, 643, 842, 888, 1030, 1107, 1145, 1229
TatI WGTACW 2 cut(s) 370, 632
TauI GCSGC 1 cut(s) 303
TfiI GAWTC 2 cut(s) 63, 820
Tru1I TTAA 1 cut(s) 440
Tru9I TTAA 1 cut(s) 440
TscAI CASTG 2 cut(s) 73, 757
TseFI GTSAC 1 cut(s) 1088
Tsp45I GTSAC 1 cut(s) 1088
TspDTI ATGAA 7 cut(s) 285, 512, 585, 700, 765, 884, 1215
TspGWI ACGGA 3 cut(s) 112, 256, 503
TspMI CCCGGG 1 cut(s) 919
TspRI CASTG 2 cut(s) 73, 757
Van91I CCANNNNNTGG 1 cut(s) 1018
VpaK11BI GGWCC 1 cut(s) 955
XapI RAATTY 5 cut(s) 306, 643, 1107, 1145, 1229
XceI RCATGY 1 cut(s) 933
XhoI CTCGAG 1 cut(s) 965
XmaI CCCGGG 1 cut(s) 919
XmiI GTMKAC 1 cut(s) 853
XspI CTAG 2 cut(s) 354, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.