Rh5AG268000

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000467
Physical Location & Seq
Reverse (-)
35370594 .. 35382588
11995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG268000.1

Sequence Viewer

Length: 1542 bp
ATGGGAAACTGTTTCACTCATAAAGAAAAAGCTTCAGATCGGTGGATCCAGGAGGAGGAGGAAGAAAACAAACACCATGAGCAGCGAAACAGAATCAGCGATCCCCCAGATCAGTGCACTGATGCTCCGGCTGACAGAATCAGCGGTGATCTTTCAGATCGGTGGAGTGAGGAGGAAGAAAACAAGCATGAGAAGAGAAACAGTACTGGTTCATCTGACAGAATCAGTGGTCTCCCAGATGATATGTTGCATCTCATTCTCTCATTCTTGCCTTTCAAATACGTTGGGCAAACTAGTGTCTTATCTCGAAGATGGAGTCATGTATGGTCTTCCTATCCCATCTTTGACTTCTACGAAATTTTTACTGGTAATGAAGCTGAACATCACCAGACAAGAGCAAGCATCATCAACACAGTATTGGCACGCGATCGTCACAACGAAAACTATAACATGATGTACCCATGCCAAAAAATTCCCTTGCCAAGCGGCAGACTAGGTCAATTTCGTATAGTTGGGGAGAATCCAAACAATTCTTGTTTGGCTAGGGTTGAGGAACTTGTACTCGATATCTCGTTGAGTAGTTCAAATACATCTAATTTGCCTCGATGTAAACTTAAGTGCCACTCATTAAGGAGTAGTAGCTGTGGGAGTTCAAAGGCATGGCTAGGGTTTTCCTCTACTTATGTTGTCTGGTCTTTTCTCCTTTCACTCCAAGATTTGTCTCTAGCTCATGTGGATTTCTCGGATAGTGCTTTGGGTGTGGATTTATTTTCTGGTTCTTCATTCCCTTTTCTCAAAAAATTGAATATAGAGCGGTGTAGAGGAATGACTGCTCTCAAAATTTGCTGTCCGAACCTAAAAGTTGTATACGTTTTCAGAATGGATCTATATAGCATGGACATCTCTGGAATGAGACTAGAGAAGTTGTCTTGTGTTGAAAATTGCATCGATGGTTGGGTCAACATTTTTTCCCCCAATCTACAATACTTATATTGGGGCAATGCAATTACTGAGAAGTGTTCCATCCAGAGCTTTCCAAAGCCCAAAAGATCTTCCCTGAGGTATTCGTTTCGGTTCGGCATTACAACAACAAAGATTTTTCATAAAAGTGTTATCAATCTTCTTTGCCATTCATCCCAAGCTGAGATTCTTGGTATATTTGATGACTACCTCGAGATTCTATCAGACATTTATACTGAATTTGGTGGTGTACCTTTCTCATTTTTCAAACTTGAAACCTTGAGAATTGGGTCCACAATGAAGCAAAGATATATCCCGGGAATAGCATGCCTGTTGAAGAGCTCTCCCTTACTTAACACCCTCTACTTTGAATTTTACTATTTCGAAGAAAATGGTAAGTGGAATAACATTATGTTGGATAATGGCAATTGCACTCAAGAGCAATACTGGGAAACTCAGGCTCAACATTTGCTCCCCTTTCTTAGTCACCTAAAGCTTGTTCTTTGGGTAAGGTTTCGGGAGCAAAGCCTTACCCCCATTTTGAATTCCTCTTCTTCGATCAGGCTTCCTTTGTACGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

513

Amino Acids

59.11

Weight (kDa)

6.58

Isoelectric Point (pI)

53.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 75 - 114 6.8e-09 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000467)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g02550 FvH4_6g26921 FvH4_6g45630
malus_domestica MD01G1113200.v1.1 MD09G1101600.v1.1 MD17G1084600.v1.1
prunus_persica Prupe.3G224400_v2.0.a1 Prupe.3G224500_v2.0.a1 Prupe.3G224600_v2.0.a1
pyrus_communis pycom02g19930 pycom09g00760 pycom09g00770 pycom17g08170
rosa_chinensis RchiOBHm_Chr1g0350511 RchiOBHm_Chr2g0086171 RchiOBHm_Chr2g0117831 RchiOBHm_Chr3g0450391 RchiOBHm_Chr4g0409631 RchiOBHm_Chr5g0039731
rosa_laevigata RLG00000008538 RLG00000018375 RLG00000021415 RLG00000028481 RLG00000033926 RLG00000033937 RLG00000033938 RLG00000033939 RLG00000033941
rosa_multiflora Rmu_sc0000103.1_g000011 Rmu_sc0000330.1_g000009 Rmu_sc0000652.1_g000002 Rmu_sc0001507.1_g000017 Rmu_sc0004020.1_g000010 Rmu_sc0026676.1_g000001 Rmu_ssc0000083.1_g000022 Rmu_ssc0000400.1_g000021
rosa_roxburghii Rroxscaffold_1G00040760 Rroxscaffold_1G00041490 Rroxscaffold_1G00041500 Rroxscaffold_1G00041630 Rroxscaffold_2G00087990 Rroxscaffold_2G00126200 Rroxscaffold_4G00304800 Rroxscaffold_5G00354160 Rroxscaffold_5G00354170
rosa_rugosa Rorug01G0211500 Rorug02G0214300 Rorug02G0496800 Rorug02G0627700 Rorug04G0095600 Rorug05G0181700
rosa_samantha Rh1AG226300 Rh1BG194700 Rh1CG211100 Rh1DG222900 Rh2AG270500 Rh2AG270600 Rh2AG562900 Rh2AG563000 Rh2BG281900 Rh2BG282000 Rh2BG575700 Rh2CG269300 Rh2CG269400 Rh2DG278300 Rh2DG296200 Rh2DG585900 Rh3AG028000 Rh3AG028100 Rh3BG028500 Rh3BG028600 Rh3CG027300 Rh3CG027400 Rh3DG028400 Rh3DG028500 Rh4BG156800 Rh4CG167300 Rh4CG167400 Rh4DG151100 Rh5AG268000 Rh5BG272400 Rh5BG272500 Rh5CG304000 Rh5CG305000 Rh5CG305100 Rh5DG280500
rosa_wichuraiana Rw1G019530 Rw2G021370 Rw4G012860 Rw4G012970 Rw5G025070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 814
AccI GTMKAC 1 cut(s) 867
AccII CGCG 1 cut(s) 426
AciI CCGC 3 cut(s) 144, 486, 814
AclWI GGATC 4 cut(s) 40, 53, 95, 891
AcsI RAATTY 6 cut(s) 357, 471, 840, 1199, 1331, 1504
AcuI CTGAAG 1 cut(s) 18
AfaI GTAC 5 cut(s) 205, 458, 561, 1212, 1535
AfiI CCNNNNNNNGG 1 cut(s) 55
AflII CTTAAG 1 cut(s) 614
AhlI ACTAGT 1 cut(s) 293
AjnI CCWGG 1 cut(s) 48
AjuI GAANNNNNNNTTGG 2 cut(s) 1131, 1163
AluBI AGCT 8 cut(s) 32, 377, 642, 728, 1032, 1142, 1302, 1456
AluI AGCT 8 cut(s) 32, 377, 642, 728, 1032, 1142, 1302, 1456
Alw21I GWGCWC 2 cut(s) 119, 1304
Alw26I GTCTC 3 cut(s) 236, 726, 907
Alw44I GTGCAC 1 cut(s) 115
AlwI GGATC 4 cut(s) 40, 53, 95, 891
Ama87I CYCGRG 2 cut(s) 1172, 1276
ApaLI GTGCAC 1 cut(s) 115
ApeKI GCWGC 1 cut(s) 82
ApoI RAATTY 6 cut(s) 357, 471, 840, 1199, 1331, 1504
ArsI GACNNNNNNTTYG 2 cut(s) 301, 333
AspS9I GGNCC 1 cut(s) 1251
AsuC2I CCSGG 2 cut(s) 1277, 1278
AsuHPI GGTGA 3 cut(s) 158, 377, 1439
AsuII TTCGAA 1 cut(s) 1344
AvaI CYCGRG 2 cut(s) 1172, 1276
AvaII GGWCC 1 cut(s) 1251
AxyI CCTNAGG 1 cut(s) 1058
BaeGI GKGCMC 1 cut(s) 119
BamHI GGATCC 1 cut(s) 45
BanII GRGCYC 1 cut(s) 1304
BbsI GAAGAC 1 cut(s) 321
Bbv12I GWGCWC 2 cut(s) 119, 1304
BbvI GCAGC 1 cut(s) 94
BccI CCATC 4 cut(s) 306, 347, 944, 1031
BciT130I CCWGG 1 cut(s) 50
BcnI CCSGG 2 cut(s) 1277, 1278
BcoDI GTCTC 3 cut(s) 236, 726, 907
BcuI ACTAGT 1 cut(s) 293
BfaI CTAG 6 cut(s) 294, 494, 543, 665, 725, 917
BfrI CTTAAG 1 cut(s) 614
BglII AGATCT 1 cut(s) 1049
BisI GCNGC 2 cut(s) 83, 487
BlsI GCNGC 2 cut(s) 84, 488
BmcAI AGTACT 1 cut(s) 205
Bme1390I CCNGG 3 cut(s) 50, 1277, 1278
Bme18I GGWCC 1 cut(s) 1251
BmeT110I CYCGRG 2 cut(s) 1172, 1276
BmgT120I GGNCC 1 cut(s) 1251
BmiI GGNNCC 2 cut(s) 47, 1252
BmrFI CCNGG 3 cut(s) 50, 1277, 1278
BmrI ACTGGG 1 cut(s) 1417
BmsI GCATC 4 cut(s) 112, 259, 411, 954
BmuI ACTGGG 1 cut(s) 1417
BpiI GAAGAC 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 1344
BpuEI CTTGAG 2 cut(s) 1261, 1380
BpuMI CCSGG 2 cut(s) 1277, 1278
Bsa29I ATCGAT 1 cut(s) 948
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 1 cut(s) 1276
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 1416, 1446
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse1I ACTGG 3 cut(s) 211, 370, 1412
Bse21I CCTNAGG 1 cut(s) 1058
Bse3DI GCAATG 1 cut(s) 1006
BseBI CCWGG 1 cut(s) 50
BseCI ATCGAT 1 cut(s) 948
BseDI CCNNGG 1 cut(s) 1276
BseGI GGATG 2 cut(s) 1023, 1133
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMI GCAATG 1 cut(s) 1006
BseMII CTCAG 4 cut(s) 1002, 1049, 1134, 1430
BseNI ACTGG 3 cut(s) 211, 370, 1412
BseRI GAGGAG 3 cut(s) 68, 71, 185
BseSI GKGCMC 1 cut(s) 119
BseXI GCAGC 1 cut(s) 94
Bsh1236I CGCG 1 cut(s) 426
Bsh1285I CGRYCG 1 cut(s) 430
BshVI ATCGAT 1 cut(s) 948
BsiEI CGRYCG 1 cut(s) 430
BsiHKAI GWGCWC 2 cut(s) 119, 1304
BsiHKCI CYCGRG 2 cut(s) 1172, 1276
BsiSI CCGG 2 cut(s) 128, 1277
BslI CCNNNNNNNGG 1 cut(s) 55
BsmAI GTCTC 3 cut(s) 236, 726, 907
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 2 cut(s) 1172, 1276
Bsp119I TTCGAA 1 cut(s) 1344
Bsp1286I GDGCHC 2 cut(s) 119, 1304
BspACI CCGC 3 cut(s) 144, 486, 814
BspCNI CTCAG 4 cut(s) 1003, 1050, 1135, 1429
BspDI ATCGAT 1 cut(s) 948
BspFNI CGCG 1 cut(s) 426
BspLI GGNNCC 2 cut(s) 47, 1252
BspPI GGATC 4 cut(s) 40, 53, 95, 891
BspQI GCTCTTC 1 cut(s) 1292
BspT104I TTCGAA 1 cut(s) 1344
BspTI CTTAAG 1 cut(s) 614
BspTNI GGTCTC 1 cut(s) 236
BsrBI CCGCTC 1 cut(s) 814
BsrDI GCAATG 1 cut(s) 1006
BsrI ACTGG 3 cut(s) 211, 370, 1412
BssECI CCNNGG 1 cut(s) 1276
BssNAI GTATAC 1 cut(s) 868
Bst1107I GTATAC 1 cut(s) 868
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 3 cut(s) 11, 203, 415
Bst6I CTCTTC 3 cut(s) 188, 1292, 1516
BstAFI CTTAAG 1 cut(s) 614
BstBI TTCGAA 1 cut(s) 1344
BstC8I GCNNGC 3 cut(s) 400, 424, 1288
BstDEI CTNAG 5 cut(s) 1011, 1058, 1143, 1416, 1442
BstF5I GGATG 2 cut(s) 1023, 1133
BstFNI CGCG 1 cut(s) 426
BstMAI GTCTC 3 cut(s) 236, 726, 907
BstMCI CGRYCG 1 cut(s) 430
BstNI CCWGG 1 cut(s) 50
BstNSI RCATGY 1 cut(s) 1290
BstSCI CCNGG 3 cut(s) 48, 1275, 1276
BstSLI GKGCMC 1 cut(s) 119
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 94
BstV2I GAAGAC 1 cut(s) 321
BstX2I RGATCY 3 cut(s) 45, 883, 1049
BstYI RGATCY 3 cut(s) 45, 883, 1049
BstZ17I GTATAC 1 cut(s) 868
Bsu15I ATCGAT 1 cut(s) 948
Bsu36I CCTNAGG 1 cut(s) 1058
BsuTUI ATCGAT 1 cut(s) 948
BtsCI GGATG 2 cut(s) 1023, 1133
BtsIMutI CAGTG 3 cut(s) 117, 119, 232
Cac8I GCNNGC 3 cut(s) 400, 424, 1288
Cfr13I GGNCC 1 cut(s) 1251
Cfr9I CCCGGG 1 cut(s) 1276
ClaI ATCGAT 1 cut(s) 948
Csp6I GTAC 5 cut(s) 204, 457, 560, 1211, 1534
CviAII CATG 9 cut(s) 77, 188, 320, 451, 462, 660, 731, 895, 1287
CviQI GTAC 5 cut(s) 204, 457, 560, 1211, 1534
DdeI CTNAG 5 cut(s) 1011, 1058, 1143, 1416, 1442
Eam1104I CTCTTC 3 cut(s) 188, 1292, 1516
EarI CTCTTC 3 cut(s) 188, 1292, 1516
Ecl136II GAGCTC 1 cut(s) 1302
Eco24I GRGCYC 1 cut(s) 1304
Eco31I GGTCTC 1 cut(s) 236
Eco32I GATATC 1 cut(s) 568
Eco47I GGWCC 1 cut(s) 1251
Eco53kI GAGCTC 1 cut(s) 1302
Eco57I CTGAAG 1 cut(s) 18
Eco81I CCTNAGG 1 cut(s) 1058
Eco88I CYCGRG 2 cut(s) 1172, 1276
EcoICRI GAGCTC 1 cut(s) 1302
EcoRI GAATTC 1 cut(s) 1504
EcoRII CCWGG 1 cut(s) 48
EcoRV GATATC 1 cut(s) 568
EcoT38I GRGCYC 1 cut(s) 1304
FaeI CATG 9 cut(s) 80, 191, 323, 454, 465, 663, 734, 898, 1290
FatI CATG 9 cut(s) 76, 187, 319, 450, 461, 659, 730, 894, 1286
FblI GTMKAC 1 cut(s) 867
Fnu4HI GCNGC 2 cut(s) 83, 487
FokI GGATG 2 cut(s) 1010, 1120
FriOI GRGCYC 1 cut(s) 1304
Fsp4HI GCNGC 2 cut(s) 83, 487
FspBI CTAG 6 cut(s) 294, 494, 543, 665, 725, 917
GluI GCNGC 2 cut(s) 83, 487
HapII CCGG 2 cut(s) 128, 1277
Hin1II CATG 9 cut(s) 80, 191, 323, 454, 465, 663, 734, 898, 1290
HincII GTYRAC 1 cut(s) 961
HindII GTYRAC 1 cut(s) 961
HindIII AAGCTT 2 cut(s) 30, 1454
HinfI GANTC 7 cut(s) 93, 138, 222, 316, 520, 1147, 1177
HpaII CCGG 2 cut(s) 128, 1277
HphI GGTGA 3 cut(s) 158, 377, 1439
Hpy166II GTNNAC 6 cut(s) 117, 611, 868, 961, 1211, 1254
Hpy188I TCNGA 7 cut(s) 37, 157, 217, 745, 852, 878, 1186
Hpy188III TCNNGA 6 cut(s) 306, 906, 1027, 1174, 1397, 1478
Hpy8I GTNNAC 6 cut(s) 117, 611, 868, 961, 1211, 1254
HpyCH4III ACNGT 3 cut(s) 11, 203, 415
HpyCH4IV ACGT 3 cut(s) 282, 870, 1536
HpyCH4V TGCA 5 cut(s) 117, 250, 945, 1004, 1392
HpyF3I CTNAG 5 cut(s) 1011, 1058, 1143, 1416, 1442
HpySE526I ACGT 3 cut(s) 282, 870, 1536
Hsp92II CATG 9 cut(s) 80, 191, 323, 454, 465, 663, 734, 898, 1290
LguI GCTCTTC 1 cut(s) 1292
LmnI GCTCC 3 cut(s) 130, 1437, 1480
Lsp1109I GCAGC 1 cut(s) 94
LweI GCATC 4 cut(s) 112, 259, 411, 954
MaeI CTAG 6 cut(s) 294, 494, 543, 665, 725, 917
MaeII ACGT 3 cut(s) 282, 870, 1536
MaeIII GTNAC 2 cut(s) 431, 1445
MbiI CCGCTC 1 cut(s) 814
MfeI CAATTG 1 cut(s) 1387
MflI RGATCY 3 cut(s) 45, 883, 1049
MhlI GDGCHC 2 cut(s) 119, 1304
MlyI GAGTC 1 cut(s) 325
MmeI TCCRAC 1 cut(s) 1356
MseI TTAA 3 cut(s) 615, 629, 1314
MslI CAYNNNNRTG 1 cut(s) 1107
MspA1I CMGCKG 1 cut(s) 144
MspCI CTTAAG 1 cut(s) 614
MspI CCGG 2 cut(s) 128, 1277
MspR9I CCNGG 3 cut(s) 50, 1277, 1278
MunI CAATTG 1 cut(s) 1387
MvaI CCWGG 1 cut(s) 50
MvnI CGCG 1 cut(s) 426
NciI CCSGG 2 cut(s) 1277, 1278
NlaIII CATG 9 cut(s) 80, 191, 323, 454, 465, 663, 734, 898, 1290
NlaIV GGNNCC 2 cut(s) 47, 1252
NmuCI GTSAC 2 cut(s) 431, 1445
NspI RCATGY 1 cut(s) 1290
NspV TTCGAA 1 cut(s) 1344
PaeI GCATGC 1 cut(s) 1290
PaeR7I CTCGAG 1 cut(s) 1172
PciSI GCTCTTC 1 cut(s) 1292
PfeI GAWTC 6 cut(s) 93, 138, 222, 520, 1147, 1177
PflFI GACNNNGTC 1 cut(s) 495
PfoI TCCNGGA 1 cut(s) 48
PkrI GCNGC 2 cut(s) 84, 488
Ple19I CGATCG 1 cut(s) 430
PleI GAGTC 1 cut(s) 324
PpsI GAGTC 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 1304
Psp6I CCWGG 1 cut(s) 48
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 2 cut(s) 47, 1252
PspPI GGNCC 1 cut(s) 1251
PsuI RGATCY 3 cut(s) 45, 883, 1049
PsyI GACNNNGTC 1 cut(s) 495
PvuI CGATCG 1 cut(s) 430
RsaI GTAC 5 cut(s) 205, 458, 561, 1212, 1535
RsaNI GTAC 5 cut(s) 204, 457, 560, 1211, 1534
RseI CAYNNNNRTG 1 cut(s) 1107
SacI GAGCTC 1 cut(s) 1304
SapI GCTCTTC 1 cut(s) 1292
SaqAI TTAA 3 cut(s) 615, 629, 1314
SatI GCNGC 2 cut(s) 83, 487
Sau96I GGNCC 1 cut(s) 1251
ScaI AGTACT 1 cut(s) 205
SchI GAGTC 1 cut(s) 325
ScrFI CCNGG 3 cut(s) 50, 1277, 1278
SduI GDGCHC 2 cut(s) 119, 1304
SfaNI GCATC 4 cut(s) 112, 259, 411, 954
Sfr274I CTCGAG 1 cut(s) 1172
SfuI TTCGAA 1 cut(s) 1344
SinI GGWCC 1 cut(s) 1251
SlaI CTCGAG 1 cut(s) 1172
SmaI CCCGGG 1 cut(s) 1278
SmiMI CAYNNNNRTG 1 cut(s) 1107
SmlI CTYRAG 4 cut(s) 614, 1172, 1240, 1395
SmoI CTYRAG 4 cut(s) 614, 1172, 1240, 1395
SpeI ACTAGT 1 cut(s) 293
SphI GCATGC 1 cut(s) 1290
SsiI CCGC 3 cut(s) 144, 486, 814
SspMI CTAG 6 cut(s) 294, 494, 543, 665, 725, 917
SstI GAGCTC 1 cut(s) 1304
StyD4I CCNGG 3 cut(s) 48, 1275, 1276
TaaI ACNGT 3 cut(s) 11, 203, 415
TaiI ACGT 3 cut(s) 285, 873, 1539
TaqI TCGA 7 cut(s) 307, 564, 604, 948, 1173, 1344, 1517
TatI WGTACW 2 cut(s) 203, 559
TauI GCSGC 1 cut(s) 489
TfiI GAWTC 6 cut(s) 93, 138, 222, 520, 1147, 1177
Tru1I TTAA 3 cut(s) 615, 629, 1314
Tru9I TTAA 3 cut(s) 615, 629, 1314
TscAI CASTG 3 cut(s) 119, 124, 232
TseFI GTSAC 2 cut(s) 431, 1445
TseI GCWGC 1 cut(s) 82
Tsp45I GTSAC 2 cut(s) 431, 1445
TspDTI ATGAA 6 cut(s) 201, 387, 771, 1091, 1122, 1274
TspMI CCCGGG 1 cut(s) 1276
TspRI CASTG 3 cut(s) 119, 124, 232
Tth111I GACNNNGTC 1 cut(s) 495
Vha464I CTTAAG 1 cut(s) 614
VneI GTGCAC 1 cut(s) 115
VpaK11BI GGWCC 1 cut(s) 1251
XapI RAATTY 6 cut(s) 357, 471, 840, 1199, 1331, 1504
XceI RCATGY 1 cut(s) 1290
XhoI CTCGAG 1 cut(s) 1172
XmaI CCCGGG 1 cut(s) 1276
XmiI GTMKAC 1 cut(s) 867
XspI CTAG 6 cut(s) 294, 494, 543, 665, 725, 917
ZrmI AGTACT 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.