Rw5G025070

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000467
Physical Location & Seq
Reverse (-)
35617532 .. 35619202
1671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G025070.1

Sequence Viewer

Length: 1560 bp
ATGGGAAACTGTTTCACTCATAAAGAAAAAGCTTCAGATCGGTGGATCCGGGAGGAGGAGGAAGAAAACAAACACCATGAGCAGCGAAACAGAATCAGCGATCCCTCAGATCAGTGCACTGATGCTCCGGCTGACAGAATCAGCGGTGATCTTTCAGATCGGTGGAGTGAGGAGGAAGAAAACAAGCATGAGAAGAGAAACAGTACTGGTTCATCTGACAGAATCAGTGATCTCCCAGATGATATGTTGCATCTCATACTCTCATTCTTGCCTTTCAAATACGTTGGGCAAACTAGTGTCTTATCTCGAAGATGGAGTCATGTATGGTCTTCCTATCCCATCTTTGACTTCTACGAGATTTTTACTGGTATTGAAGCTGAACATCACCAGACAAGAGCAAGCATCATCAACACGGTATTGGCACGCGATCGTCACAACGAAAACTATAACATGATGTACCCATGCCAAAAAATTCCCTTGCCAAGCGGCAGACTAGGTCAATTTCGTATAGTTGGGGAGAATCCAAACAATTCTTGTTTGGCTAGGGTTGAGGAACTTGTACTCGATATCTCGTTGAGTAGTGCAAATACATCTAATTTGCCTCGATGTCAACTTAAGTGCCACTCATTAACGAGTAGTAGCTGTGGGAGTTCAAAGGCATGGCTAGGGTTTTCCTCTCCTTATGTTGTCTGGTCTTTTCTCCTTTCACTCCAAGATTTGTCTCTAGCTCATGTGGATTTCTCGGATAGTGCTTTGGGTGTGGATTTATTTTCTGGTTCTTCATTCCCTTTTCTCAAAAAATTGAATATAGAGCGGTGTAGAGGAATGACTGCTCTCAAAATTTGCTGTCCGAACCTAAAAGTTGTATATGTTTTCAGAATGGATCTATATAGCATGGACATCTCTGGAATGAGACTAGAGAAGTTGTCTTGTGTTGAAAATTGCATCGATGGTTGGGTCAACATTTTTTCCCCCAATCTACAATACTTATATTGGGGCAATGCAATTACTGAGAAGTGTTCCATCCAGAGCTTTCCAAAGCCCAAAAGATCTTCCCTGAGTTATTCTTTTCGGTTAGGCATTACAACAACAAAGATTTTTCATAAAAGTATTCTATCAGACATTTATACTGAATTTGGTGGTGTACCTTTCTCATTTTTCAAACTTGAAACCTTGAGAATTGGGTCCACAATGAAGCAAAGATATATCCCAGGAATAGCATGCCTGTTGAAGAGCTCTCCCTTACTTAACACCCTCTATTTTGAATTTTACTATTTCGAAGAAAATGAGCAATACTGGGAAACTCAGGCTCAACATTTGCTCCCCTTTCTTAGTCACCTAAAGGTGGTCGACATTGGCCTTGGTAACATGATCTCTGAAAGTGCAATCACTTTTGCAAAGTTTTTGCTTAAATATGGAAGAGGCCTGCAAAAAATGACTCTTAGCTTTTGGAGGAGCGGAAGTTCACTTTCTCCAAATTCGCTAAATGATACCATTGATTTATTAAAGGGTTTTCCCCGGGCATCTGCAGATGTTGAGTTCTCAAGATTCTGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

519

Amino Acids

59.43

Weight (kDa)

6.71

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 75 - 114 1.4e-09 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000467)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g02550 FvH4_6g26921 FvH4_6g45630
malus_domestica MD01G1113200.v1.1 MD09G1101600.v1.1 MD17G1084600.v1.1
prunus_persica Prupe.3G224400_v2.0.a1 Prupe.3G224500_v2.0.a1 Prupe.3G224600_v2.0.a1
pyrus_communis pycom02g19930 pycom09g00760 pycom09g00770 pycom17g08170
rosa_chinensis RchiOBHm_Chr1g0350511 RchiOBHm_Chr2g0086171 RchiOBHm_Chr2g0117831 RchiOBHm_Chr3g0450391 RchiOBHm_Chr4g0409631 RchiOBHm_Chr5g0039731
rosa_laevigata RLG00000008538 RLG00000018375 RLG00000021415 RLG00000028481 RLG00000033926 RLG00000033937 RLG00000033938 RLG00000033939 RLG00000033941
rosa_multiflora Rmu_sc0000103.1_g000011 Rmu_sc0000330.1_g000009 Rmu_sc0000652.1_g000002 Rmu_sc0001507.1_g000017 Rmu_sc0004020.1_g000010 Rmu_sc0026676.1_g000001 Rmu_ssc0000083.1_g000022 Rmu_ssc0000400.1_g000021
rosa_roxburghii Rroxscaffold_1G00040760 Rroxscaffold_1G00041490 Rroxscaffold_1G00041500 Rroxscaffold_1G00041630 Rroxscaffold_2G00087990 Rroxscaffold_2G00126200 Rroxscaffold_4G00304800 Rroxscaffold_5G00354160 Rroxscaffold_5G00354170
rosa_rugosa Rorug01G0211500 Rorug02G0214300 Rorug02G0496800 Rorug02G0627700 Rorug04G0095600 Rorug05G0181700
rosa_samantha Rh1AG226300 Rh1BG194700 Rh1CG211100 Rh1DG222900 Rh2AG270500 Rh2AG270600 Rh2AG562900 Rh2AG563000 Rh2BG281900 Rh2BG282000 Rh2BG575700 Rh2CG269300 Rh2CG269400 Rh2DG278300 Rh2DG296200 Rh2DG585900 Rh3AG028000 Rh3AG028100 Rh3BG028500 Rh3BG028600 Rh3CG027300 Rh3CG027400 Rh3DG028400 Rh3DG028500 Rh4BG156800 Rh4CG167300 Rh4CG167400 Rh4DG151100 Rh5AG268000 Rh5BG272400 Rh5BG272500 Rh5CG304000 Rh5CG305000 Rh5CG305100 Rh5DG280500
rosa_wichuraiana Rw1G019530 Rw2G021370 Rw4G012860 Rw4G012970 Rw5G025070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 814, 1458
AccI GTMKAC 1 cut(s) 1350
AccII CGCG 1 cut(s) 426
AciI CCGC 4 cut(s) 144, 486, 814, 1458
AclWI GGATC 4 cut(s) 40, 53, 95, 891
AcsI RAATTY 5 cut(s) 471, 840, 1133, 1265, 1477
AcuI CTGAAG 1 cut(s) 18
AfaI GTAC 4 cut(s) 205, 458, 561, 1146
AfiI CCNNNNNNNGG 2 cut(s) 55, 1345
AflII CTTAAG 1 cut(s) 614
AgsI TTSAA 9 cut(s) 277, 374, 654, 805, 938, 1162, 1169, 1231, 1265
AhlI ACTAGT 1 cut(s) 293
AjnI CCWGG 1 cut(s) 1210
AloI GAACNNNNNNTCC 2 cut(s) 1447, 1479
AluBI AGCT 7 cut(s) 32, 377, 642, 728, 1032, 1236, 1446
AluI AGCT 7 cut(s) 32, 377, 642, 728, 1032, 1236, 1446
Alw21I GWGCWC 2 cut(s) 119, 1238
Alw26I GTCTC 2 cut(s) 726, 907
Alw44I GTGCAC 1 cut(s) 115
AlwI GGATC 4 cut(s) 40, 53, 95, 891
Ama87I CYCGRG 1 cut(s) 1518
AoxI GGCC 2 cut(s) 1357, 1423
ApaLI GTGCAC 1 cut(s) 115
ApeKI GCWGC 1 cut(s) 82
ApoI RAATTY 5 cut(s) 471, 840, 1133, 1265, 1477
ArsI GACNNNNNNTTYG 2 cut(s) 301, 333
AspS9I GGNCC 1 cut(s) 1185
AsuC2I CCSGG 3 cut(s) 50, 1519, 1520
AsuHPI GGTGA 3 cut(s) 158, 377, 1328
AsuII TTCGAA 1 cut(s) 1278
AvaI CYCGRG 1 cut(s) 1518
AvaII GGWCC 1 cut(s) 1185
BaeGI GKGCMC 1 cut(s) 119
BamHI GGATCC 1 cut(s) 45
BanII GRGCYC 1 cut(s) 1238
BbsI GAAGAC 1 cut(s) 321
Bbv12I GWGCWC 2 cut(s) 119, 1238
BbvI GCAGC 1 cut(s) 94
BccI CCATC 4 cut(s) 306, 347, 944, 1031
BciT130I CCWGG 1 cut(s) 1212
BcnI CCSGG 3 cut(s) 50, 1519, 1520
BcoDI GTCTC 2 cut(s) 726, 907
BcuI ACTAGT 1 cut(s) 293
BfaI CTAG 6 cut(s) 294, 494, 543, 665, 725, 917
BfmI CTRYAG 1 cut(s) 1527
BfrI CTTAAG 1 cut(s) 614
BglII AGATCT 1 cut(s) 1049
BisI GCNGC 2 cut(s) 83, 487
BlsI GCNGC 2 cut(s) 84, 488
BmcAI AGTACT 1 cut(s) 205
Bme1390I CCNGG 4 cut(s) 50, 1212, 1519, 1520
Bme18I GGWCC 1 cut(s) 1185
BmeT110I CYCGRG 1 cut(s) 1518
BmgT120I GGNCC 1 cut(s) 1185
BmiI GGNNCC 2 cut(s) 47, 1186
BmrFI CCNGG 4 cut(s) 50, 1212, 1519, 1520
BmrI ACTGGG 1 cut(s) 1306
BmsI GCATC 5 cut(s) 112, 259, 411, 954, 1532
BmuI ACTGGG 1 cut(s) 1306
BpiI GAAGAC 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 1278
BpuEI CTTGAG 2 cut(s) 1195, 1528
BpuMI CCSGG 3 cut(s) 50, 1519, 1520
Bsa29I ATCGAT 1 cut(s) 948
BsaJI CCNNGG 4 cut(s) 1210, 1360, 1517, 1518
BsaXI ACNNNNNCTCC 6 cut(s) 109, 139, 1305, 1335, 1447, 1477
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 1345
Bse1I ACTGG 3 cut(s) 211, 370, 1301
Bse3DI GCAATG 1 cut(s) 1006
BseBI CCWGG 1 cut(s) 1212
BseCI ATCGAT 1 cut(s) 948
BseDI CCNNGG 4 cut(s) 1210, 1360, 1517, 1518
BseGI GGATG 1 cut(s) 1023
BseLI CCNNNNNNNGG 2 cut(s) 55, 1345
BseMI GCAATG 1 cut(s) 1006
BseMII CTCAG 4 cut(s) 120, 1002, 1049, 1319
BseNI ACTGG 3 cut(s) 211, 370, 1301
BseRI GAGGAG 4 cut(s) 68, 71, 185, 1468
BseSI GKGCMC 1 cut(s) 119
BseXI GCAGC 1 cut(s) 94
Bsh1236I CGCG 1 cut(s) 426
Bsh1285I CGRYCG 1 cut(s) 430
BshFI GGCC 2 cut(s) 1359, 1425
BshVI ATCGAT 1 cut(s) 948
BsiEI CGRYCG 1 cut(s) 430
BsiHKAI GWGCWC 2 cut(s) 119, 1238
BsiHKCI CYCGRG 1 cut(s) 1518
BsiSI CCGG 3 cut(s) 49, 128, 1519
BslI CCNNNNNNNGG 2 cut(s) 55, 1345
BsmAI GTCTC 2 cut(s) 726, 907
BsnI GGCC 2 cut(s) 1359, 1425
BsoBI CYCGRG 1 cut(s) 1518
Bsp119I TTCGAA 1 cut(s) 1278
Bsp1286I GDGCHC 2 cut(s) 119, 1238
BspACI CCGC 4 cut(s) 144, 486, 814, 1458
BspANI GGCC 2 cut(s) 1359, 1425
BspCNI CTCAG 4 cut(s) 119, 1003, 1050, 1318
BspDI ATCGAT 1 cut(s) 948
BspFNI CGCG 1 cut(s) 426
BspLI GGNNCC 2 cut(s) 47, 1186
BspMAI CTGCAG 1 cut(s) 1531
BspPI GGATC 4 cut(s) 40, 53, 95, 891
BspQI GCTCTTC 1 cut(s) 1226
BspT104I TTCGAA 1 cut(s) 1278
BspTI CTTAAG 1 cut(s) 614
BsrBI CCGCTC 2 cut(s) 814, 1458
BsrDI GCAATG 1 cut(s) 1006
BsrI ACTGG 3 cut(s) 211, 370, 1301
BssECI CCNNGG 4 cut(s) 1210, 1360, 1517, 1518
BssT1I CCWWGG 1 cut(s) 1360
Bst2UI CCWGG 1 cut(s) 1212
Bst4CI ACNGT 3 cut(s) 11, 203, 415
Bst6I CTCTTC 3 cut(s) 188, 1226, 1414
BstAFI CTTAAG 1 cut(s) 614
BstBI TTCGAA 1 cut(s) 1278
BstC8I GCNNGC 4 cut(s) 400, 424, 1222, 1427
BstDEI CTNAG 6 cut(s) 106, 1011, 1058, 1305, 1331, 1442
BstF5I GGATG 1 cut(s) 1023
BstFNI CGCG 1 cut(s) 426
BstMAI GTCTC 2 cut(s) 726, 907
BstMCI CGRYCG 1 cut(s) 430
BstNI CCWGG 1 cut(s) 1212
BstNSI RCATGY 1 cut(s) 1224
BstSCI CCNGG 4 cut(s) 48, 1210, 1517, 1518
BstSFI CTRYAG 1 cut(s) 1527
BstSLI GKGCMC 1 cut(s) 119
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 94
BstV2I GAAGAC 1 cut(s) 321
BstX2I RGATCY 3 cut(s) 45, 883, 1049
BstYI RGATCY 3 cut(s) 45, 883, 1049
Bsu15I ATCGAT 1 cut(s) 948
BsuRI GGCC 2 cut(s) 1359, 1425
BsuTUI ATCGAT 1 cut(s) 948
BtsCI GGATG 1 cut(s) 1023
BtsIMutI CAGTG 3 cut(s) 117, 119, 232
Cac8I GCNNGC 4 cut(s) 400, 424, 1222, 1427
Cfr13I GGNCC 1 cut(s) 1185
Cfr9I CCCGGG 1 cut(s) 1518
ClaI ATCGAT 1 cut(s) 948
Csp6I GTAC 4 cut(s) 204, 457, 560, 1145
CviQI GTAC 4 cut(s) 204, 457, 560, 1145
DdeI CTNAG 6 cut(s) 106, 1011, 1058, 1305, 1331, 1442
Eam1104I CTCTTC 3 cut(s) 188, 1226, 1414
EarI CTCTTC 3 cut(s) 188, 1226, 1414
Ecl136II GAGCTC 1 cut(s) 1236
Eco130I CCWWGG 1 cut(s) 1360
Eco147I AGGCCT 1 cut(s) 1425
Eco24I GRGCYC 1 cut(s) 1238
Eco32I GATATC 1 cut(s) 568
Eco47I GGWCC 1 cut(s) 1185
Eco53kI GAGCTC 1 cut(s) 1236
Eco57I CTGAAG 1 cut(s) 18
Eco88I CYCGRG 1 cut(s) 1518
EcoICRI GAGCTC 1 cut(s) 1236
EcoRII CCWGG 1 cut(s) 1210
EcoRV GATATC 1 cut(s) 568
EcoT14I CCWWGG 1 cut(s) 1360
EcoT38I GRGCYC 1 cut(s) 1238
ErhI CCWWGG 1 cut(s) 1360
FblI GTMKAC 1 cut(s) 1350
Fnu4HI GCNGC 2 cut(s) 83, 487
FokI GGATG 1 cut(s) 1010
FriOI GRGCYC 1 cut(s) 1238
Fsp4HI GCNGC 2 cut(s) 83, 487
FspBI CTAG 6 cut(s) 294, 494, 543, 665, 725, 917
GluI GCNGC 2 cut(s) 83, 487
HaeIII GGCC 2 cut(s) 1359, 1425
HapII CCGG 3 cut(s) 49, 128, 1519
HincII GTYRAC 3 cut(s) 611, 961, 1351
HindII GTYRAC 3 cut(s) 611, 961, 1351
HindIII AAGCTT 1 cut(s) 30
HinfI GANTC 7 cut(s) 93, 138, 222, 316, 520, 1438, 1548
HpaII CCGG 3 cut(s) 49, 128, 1519
HphI GGTGA 3 cut(s) 158, 377, 1328
Hpy166II GTNNAC 7 cut(s) 117, 611, 961, 1145, 1188, 1351, 1466
Hpy188I TCNGA 9 cut(s) 37, 109, 157, 217, 745, 852, 878, 1120, 1378
Hpy188III TCNNGA 4 cut(s) 306, 906, 1027, 1545
Hpy8I GTNNAC 7 cut(s) 117, 611, 961, 1145, 1188, 1351, 1466
HpyCH4III ACNGT 3 cut(s) 11, 203, 415
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 9 cut(s) 117, 250, 584, 945, 1004, 1385, 1397, 1429, 1529
HpyF3I CTNAG 6 cut(s) 106, 1011, 1058, 1305, 1331, 1442
HpySE526I ACGT 1 cut(s) 282
LguI GCTCTTC 1 cut(s) 1226
LmnI GCTCC 3 cut(s) 130, 1326, 1455
Lsp1109I GCAGC 1 cut(s) 94
LweI GCATC 5 cut(s) 112, 259, 411, 954, 1532
MaeI CTAG 6 cut(s) 294, 494, 543, 665, 725, 917
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 3 cut(s) 431, 1334, 1364
MbiI CCGCTC 2 cut(s) 814, 1458
MflI RGATCY 3 cut(s) 45, 883, 1049
MhlI GDGCHC 2 cut(s) 119, 1238
MlyI GAGTC 2 cut(s) 325, 1432
MseI TTAA 5 cut(s) 615, 629, 1248, 1410, 1505
MspA1I CMGCKG 1 cut(s) 144
MspCI CTTAAG 1 cut(s) 614
MspI CCGG 3 cut(s) 49, 128, 1519
MspR9I CCNGG 4 cut(s) 50, 1212, 1519, 1520
MvaI CCWGG 1 cut(s) 1212
MvnI CGCG 1 cut(s) 426
NciI CCSGG 3 cut(s) 50, 1519, 1520
NlaIV GGNNCC 2 cut(s) 47, 1186
NmuCI GTSAC 2 cut(s) 431, 1334
NspI RCATGY 1 cut(s) 1224
NspV TTCGAA 1 cut(s) 1278
PaeI GCATGC 1 cut(s) 1224
PceI AGGCCT 1 cut(s) 1425
PciSI GCTCTTC 1 cut(s) 1226
PfeI GAWTC 5 cut(s) 93, 138, 222, 520, 1548
PflFI GACNNNGTC 1 cut(s) 495
PfoI TCCNGGA 1 cut(s) 48
PkrI GCNGC 2 cut(s) 84, 488
Ple19I CGATCG 1 cut(s) 430
PleI GAGTC 2 cut(s) 324, 1432
PpsI GAGTC 2 cut(s) 324, 1432
Psp124BI GAGCTC 1 cut(s) 1238
Psp6I CCWGG 1 cut(s) 1210
PspGI CCWGG 1 cut(s) 1210
PspN4I GGNNCC 2 cut(s) 47, 1186
PspPI GGNCC 1 cut(s) 1185
PstI CTGCAG 1 cut(s) 1531
PsuI RGATCY 3 cut(s) 45, 883, 1049
PsyI GACNNNGTC 1 cut(s) 495
PvuI CGATCG 1 cut(s) 430
RsaI GTAC 4 cut(s) 205, 458, 561, 1146
RsaNI GTAC 4 cut(s) 204, 457, 560, 1145
SacI GAGCTC 1 cut(s) 1238
SalI GTCGAC 1 cut(s) 1349
SapI GCTCTTC 1 cut(s) 1226
SaqAI TTAA 5 cut(s) 615, 629, 1248, 1410, 1505
SatI GCNGC 2 cut(s) 83, 487
Sau96I GGNCC 1 cut(s) 1185
ScaI AGTACT 1 cut(s) 205
SchI GAGTC 2 cut(s) 325, 1432
ScrFI CCNGG 4 cut(s) 50, 1212, 1519, 1520
SduI GDGCHC 2 cut(s) 119, 1238
SfaNI GCATC 5 cut(s) 112, 259, 411, 954, 1532
SfcI CTRYAG 1 cut(s) 1527
SfuI TTCGAA 1 cut(s) 1278
SinI GGWCC 1 cut(s) 1185
SmaI CCCGGG 1 cut(s) 1520
SmlI CTYRAG 3 cut(s) 614, 1174, 1543
SmoI CTYRAG 3 cut(s) 614, 1174, 1543
SpeI ACTAGT 1 cut(s) 293
SphI GCATGC 1 cut(s) 1224
SseBI AGGCCT 1 cut(s) 1425
SsiI CCGC 4 cut(s) 144, 486, 814, 1458
SspMI CTAG 6 cut(s) 294, 494, 543, 665, 725, 917
SstI GAGCTC 1 cut(s) 1238
StuI AGGCCT 1 cut(s) 1425
StyD4I CCNGG 4 cut(s) 48, 1210, 1517, 1518
StyI CCWWGG 1 cut(s) 1360
TaaI ACNGT 3 cut(s) 11, 203, 415
TaiI ACGT 1 cut(s) 285
TaqI TCGA 6 cut(s) 307, 564, 604, 948, 1278, 1350
TatI WGTACW 2 cut(s) 203, 559
TauI GCSGC 1 cut(s) 489
TfiI GAWTC 5 cut(s) 93, 138, 222, 520, 1548
Tru1I TTAA 5 cut(s) 615, 629, 1248, 1410, 1505
Tru9I TTAA 5 cut(s) 615, 629, 1248, 1410, 1505
TscAI CASTG 3 cut(s) 119, 124, 232
TseFI GTSAC 2 cut(s) 431, 1334
TseI GCWGC 1 cut(s) 82
Tsp45I GTSAC 2 cut(s) 431, 1334
TspDTI ATGAA 4 cut(s) 201, 771, 1091, 1208
TspMI CCCGGG 1 cut(s) 1518
TspRI CASTG 3 cut(s) 119, 124, 232
Tth111I GACNNNGTC 1 cut(s) 495
Vha464I CTTAAG 1 cut(s) 614
VneI GTGCAC 1 cut(s) 115
VpaK11BI GGWCC 1 cut(s) 1185
XapI RAATTY 5 cut(s) 471, 840, 1133, 1265, 1477
XceI RCATGY 1 cut(s) 1224
XmaI CCCGGG 1 cut(s) 1518
XmiI GTMKAC 1 cut(s) 1350
XspI CTAG 6 cut(s) 294, 494, 543, 665, 725, 917
ZrmI AGTACT 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.