Rh2DG296200

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000467
Physical Location & Seq
Forward (+)
35168151 .. 35169692
1542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG296200.1

Sequence Viewer

Length: 1488 bp
ATGGGAAACATCTTTGCCAACTGTTTTACTTGTAAACAAAAGAGTGGTTCTTCAGCTGACAGAATCAGTGATCTCCCAGATGATATATTGCATGTCATACTCTCATTCTTACCTTTCAAATCCGTTGGGCAAACAGCTATCTTATCTCGAAGATGGAATGATGTATGGTCTTCCTATCCCATCATCGACTTCTACGAGATTTTTACTGGTAGTGAAGCTGATCATCACCAGACAAAAACAAGGATCATCAACACAATATTAGCTCGCCATGAAGAAAACTATAATATACTGTACCCATGCCGCAAAATTTCCTTGCCAAGAGTTGGGGGGGTTCCGAACAAATCTTGTTTGTCTAGGGTTGAGGAACTTGTACTCGACATCAGGTTGAGTTCTGGAAACACATCTGATTTGCCTCAATGTCAACTCATGTGCCACTCATTAAGGAGTAGTAGCTGTGGGAATTCAAAGGCATGGCTAGGGTTTCCCTCGTCTTATGTTGTCGGGTCTTATCTCCGTTCACTTCATACTTTGTCTCTAACTCGTGTGGATTTCTTGGATAGTAGTGCTTTGGGTGTGGATTTATTTGCTGGTTCTTCATTCCCTTATCTCGAAAAGTTGAACTTAGAGCGCTGTAGAGGGAGGAGTGATCTCAAAATTTGCTGTCCAAACGTAAAACATGTAGAGGTATTTGATATGGAATTAAATAGTCTGGACATCTCCGGAATGAGACTGGAGGAGTTGCGAGTCTGGTTTTGTTTTCAAGAGTATATCAATGGAAGTTGGGTCAACATTTTTGAACCGAATCTACAATCATTCTTTTGGGGTTATTCAGGCATTACTGAAAGGTGTTCGATCCAGAGCTTTCCTATGCTCAAAAGATCTTTCATAAAGTCCCCCCTTACAACGAAGAAGATTAATAATAACAGTGTTGTCGATCTTCTTTCTGCTTCATCCCAAGTTGAAGACTTTATAATATCCGATCACTACCTCCAGATTCTATCAGAAATATATTCTGAATTAGGCGGTCTACCATTCTCATTTATGAAACTGAAAACCTTGAGTATTGGGAGTCCAATGAAGCAAAGATATATCCCTGGTATGGCATGCCTGTTCAAGAGCTCTCCCGTGGTCCACAACCTCGAGATTTGTTTTTACTCTTACGAAGAAGATGGCAAGTGGAACAACCAATCCTTGGATGATGCCAATTGCACCCAAGAACAATACTGGGAGACTCAGGGTCAATATTTGAGCCCCTCTCTCAGTCACCTAAAGGTGGTCAGAATTCGCCTTGGTCAAATGATCCCCGAGAGTGCAATCAAATTTGCAAAGTTTGTGCTCAAATGTGGAAGAAACCTGCAAAAAATGAGTGTTAGCTTTGATATGAACAAAAGTTGTCTACCTCCCAATTCGCTGAGTGATACCATTGCTTTAATAAAGAGTTTCCGCCGGGCATCTCCATATATCAAATTCTCAACTCATTGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

495

Amino Acids

56.29

Weight (kDa)

8.33

Isoelectric Point (pI)

56.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 60 1.8e-09 F-box domain
F-box-like PF12937 22 - 55 7.3e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000467)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g02550 FvH4_6g26921 FvH4_6g45630
malus_domestica MD01G1113200.v1.1 MD09G1101600.v1.1 MD17G1084600.v1.1
prunus_persica Prupe.3G224400_v2.0.a1 Prupe.3G224500_v2.0.a1 Prupe.3G224600_v2.0.a1
pyrus_communis pycom02g19930 pycom09g00760 pycom09g00770 pycom17g08170
rosa_chinensis RchiOBHm_Chr1g0350511 RchiOBHm_Chr2g0086171 RchiOBHm_Chr2g0117831 RchiOBHm_Chr3g0450391 RchiOBHm_Chr4g0409631 RchiOBHm_Chr5g0039731
rosa_laevigata RLG00000008538 RLG00000018375 RLG00000021415 RLG00000028481 RLG00000033926 RLG00000033937 RLG00000033938 RLG00000033939 RLG00000033941
rosa_multiflora Rmu_sc0000103.1_g000011 Rmu_sc0000330.1_g000009 Rmu_sc0000652.1_g000002 Rmu_sc0001507.1_g000017 Rmu_sc0004020.1_g000010 Rmu_sc0026676.1_g000001 Rmu_ssc0000083.1_g000022 Rmu_ssc0000400.1_g000021
rosa_roxburghii Rroxscaffold_1G00040760 Rroxscaffold_1G00041490 Rroxscaffold_1G00041500 Rroxscaffold_1G00041630 Rroxscaffold_2G00087990 Rroxscaffold_2G00126200 Rroxscaffold_4G00304800 Rroxscaffold_5G00354160 Rroxscaffold_5G00354170
rosa_rugosa Rorug01G0211500 Rorug02G0214300 Rorug02G0496800 Rorug02G0627700 Rorug04G0095600 Rorug05G0181700
rosa_samantha Rh1AG226300 Rh1BG194700 Rh1CG211100 Rh1DG222900 Rh2AG270500 Rh2AG270600 Rh2AG562900 Rh2AG563000 Rh2BG281900 Rh2BG282000 Rh2BG575700 Rh2CG269300 Rh2CG269400 Rh2DG278300 Rh2DG296200 Rh2DG585900 Rh3AG028000 Rh3AG028100 Rh3BG028500 Rh3BG028600 Rh3CG027300 Rh3CG027400 Rh3DG028400 Rh3DG028500 Rh4BG156800 Rh4CG167300 Rh4CG167400 Rh4DG151100 Rh5AG268000 Rh5BG272400 Rh5BG272500 Rh5CG304000 Rh5CG305000 Rh5CG305100 Rh5DG280500
rosa_wichuraiana Rw1G019530 Rw2G021370 Rw4G012860 Rw4G012970 Rw5G025070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 971
Acc36I ACCTGC 1 cut(s) 1362
AccB7I CCANNNNNTGG 2 cut(s) 323, 1192
AccI GTMKAC 2 cut(s) 1027, 1396
AccIII TCCGGA 1 cut(s) 719
AciI CCGC 3 cut(s) 301, 1023, 1444
AclWI GGATC 3 cut(s) 251, 847, 1294
AcsI RAATTY 6 cut(s) 306, 460, 654, 1281, 1319, 1466
AcuI CTGAAG 1 cut(s) 36
AfaI GTAC 2 cut(s) 293, 372
AfeI AGCGCT 1 cut(s) 629
AfiI CCNNNNNNNGG 4 cut(s) 323, 1099, 1192, 1273
AflIII ACRYGT 1 cut(s) 676
AgsI TTSAA 7 cut(s) 118, 465, 619, 761, 797, 962, 1114
AhdI GACNNNNNGTC 1 cut(s) 1236
AjnI CCWGG 1 cut(s) 1093
AloI GAACNNNNNNTCC 2 cut(s) 1172, 1204
AluBI AGCT 8 cut(s) 56, 137, 218, 263, 453, 861, 1119, 1374
AluI AGCT 8 cut(s) 56, 137, 218, 263, 453, 861, 1119, 1374
Alw21I GWGCWC 2 cut(s) 1121, 1338
Alw26I GTCTC 3 cut(s) 537, 721, 1223
AlwI GGATC 3 cut(s) 251, 847, 1294
Ama87I CYCGRG 2 cut(s) 1139, 1304
Aor13HI TCCGGA 1 cut(s) 719
Aor51HI AGCGCT 1 cut(s) 629
ApoI RAATTY 6 cut(s) 306, 460, 654, 1281, 1319, 1466
AseI ATTAAT 1 cut(s) 915
AspLEI GCGC 1 cut(s) 630
AspS9I GGNCC 1 cut(s) 1129
AsuC2I CCSGG 1 cut(s) 1448
AsuHPI GGTGA 2 cut(s) 218, 1256
AvaI CYCGRG 2 cut(s) 1139, 1304
AvaII GGWCC 1 cut(s) 1129
BanII GRGCYC 2 cut(s) 1121, 1253
BauI CACGAG 1 cut(s) 540
BbsI GAAGAC 2 cut(s) 162, 969
Bbv12I GWGCWC 2 cut(s) 1121, 1338
BccI CCATC 3 cut(s) 147, 188, 1163
BciT130I CCWGG 1 cut(s) 1095
BclI TGATCA 1 cut(s) 220
BcnI CCSGG 1 cut(s) 1448
BcoDI GTCTC 3 cut(s) 537, 721, 1223
BfaI CTAG 2 cut(s) 354, 476
BfmI CTRYAG 1 cut(s) 631
BfoI RGCGCY 1 cut(s) 631
BfuAI ACCTGC 1 cut(s) 1362
BglII AGATCT 1 cut(s) 878
BisI GCNGC 1 cut(s) 301
BlsI GCNGC 1 cut(s) 302
Bme1390I CCNGG 2 cut(s) 1095, 1448
Bme18I GGWCC 1 cut(s) 1129
BmeRI GACNNNNNGTC 1 cut(s) 1236
BmeT110I CYCGRG 2 cut(s) 1139, 1304
BmgT120I GGNCC 1 cut(s) 1129
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 2 cut(s) 1095, 1448
BmrI ACTGGG 1 cut(s) 1234
BmsI GCATC 2 cut(s) 1189, 1460
BmuI ACTGGG 1 cut(s) 1234
BpiI GAAGAC 2 cut(s) 162, 969
BplI GAGNNNNNCTC 2 cut(s) 1240, 1272
BpmI CTGGAG 2 cut(s) 752, 974
BpuEI CTTGAG 1 cut(s) 1078
BpuMI CCSGG 1 cut(s) 1448
BsaJI CCNNGG 4 cut(s) 1093, 1125, 1191, 1288
BsaWI WCCGGW 1 cut(s) 719
Bsc4I CCNNNNNNNGG 4 cut(s) 323, 1099, 1192, 1273
Bse1I ACTGG 3 cut(s) 211, 735, 1229
Bse3DI GCAATG 2 cut(s) 1422, 1477
BseAI TCCGGA 1 cut(s) 719
BseBI CCWGG 1 cut(s) 1095
BseDI CCNNGG 4 cut(s) 1093, 1125, 1191, 1288
BseGI GGATG 2 cut(s) 950, 1201
BseLI CCNNNNNNNGG 4 cut(s) 323, 1099, 1192, 1273
BseMI GCAATG 2 cut(s) 1422, 1477
BseMII CTCAG 3 cut(s) 1247, 1273, 1403
BseNI ACTGG 3 cut(s) 211, 735, 1229
BseRI GAGGAG 2 cut(s) 655, 749
BsiHKAI GWGCWC 2 cut(s) 1121, 1338
BsiHKCI CYCGRG 2 cut(s) 1139, 1304
BsiSI CCGG 2 cut(s) 720, 1447
BslFI GGGAC 1 cut(s) 877
BslI CCNNNNNNNGG 4 cut(s) 323, 1099, 1192, 1273
BsmAI GTCTC 3 cut(s) 537, 721, 1223
BsmFI GGGAC 1 cut(s) 877
BsoBI CYCGRG 2 cut(s) 1139, 1304
Bsp1286I GDGCHC 3 cut(s) 1121, 1253, 1338
Bsp13I TCCGGA 1 cut(s) 719
Bsp143I GATC 9 cut(s) 70, 220, 243, 646, 852, 878, 934, 979, 1299
BspACI CCGC 3 cut(s) 301, 1023, 1444
BspCNI CTCAG 3 cut(s) 1246, 1272, 1404
BspEI TCCGGA 1 cut(s) 719
BspLI GGNNCC 1 cut(s) 333
BspMI ACCTGC 1 cut(s) 1362
BspPI GGATC 3 cut(s) 251, 847, 1294
BsrDI GCAATG 2 cut(s) 1422, 1477
BsrI ACTGG 3 cut(s) 211, 735, 1229
BssECI CCNNGG 4 cut(s) 1093, 1125, 1191, 1288
BssMI GATC 9 cut(s) 70, 220, 243, 646, 852, 878, 934, 979, 1299
BssSI CACGAG 1 cut(s) 540
BssT1I CCWWGG 2 cut(s) 1191, 1288
Bst2BI CACGAG 1 cut(s) 540
Bst2UI CCWGG 1 cut(s) 1095
Bst4CI ACNGT 3 cut(s) 23, 291, 926
BstC8I GCNNGC 2 cut(s) 265, 1105
BstDEI CTNAG 4 cut(s) 622, 1233, 1259, 1412
BstDSI CCRYGG 1 cut(s) 1125
BstF5I GGATG 2 cut(s) 950, 1201
BstH2I RGCGCY 1 cut(s) 631
BstHHI GCGC 1 cut(s) 630
BstKTI GATC 9 cut(s) 73, 223, 246, 649, 855, 881, 937, 982, 1302
BstMAI GTCTC 3 cut(s) 537, 721, 1223
BstMBI GATC 9 cut(s) 70, 220, 243, 646, 852, 878, 934, 979, 1299
BstNI CCWGG 1 cut(s) 1095
BstNSI RCATGY 3 cut(s) 95, 680, 1107
BstSCI CCNGG 2 cut(s) 1093, 1446
BstSFI CTRYAG 1 cut(s) 631
BstV2I GAAGAC 2 cut(s) 162, 969
BstX2I RGATCY 1 cut(s) 878
BstYI RGATCY 1 cut(s) 878
BtgI CCRYGG 1 cut(s) 1125
BtsCI GGATG 2 cut(s) 950, 1201
BtsIMutI CAGTG 2 cut(s) 73, 931
BveI ACCTGC 1 cut(s) 1362
Cac8I GCNNGC 2 cut(s) 265, 1105
CfoI GCGC 1 cut(s) 630
Cfr13I GGNCC 1 cut(s) 1129
Csp6I GTAC 2 cut(s) 292, 371
CviAII CATG 7 cut(s) 92, 269, 297, 427, 471, 677, 1104
CviQI GTAC 2 cut(s) 292, 371
DdeI CTNAG 4 cut(s) 622, 1233, 1259, 1412
DpnI GATC 9 cut(s) 72, 222, 245, 648, 854, 880, 936, 981, 1301
DpnII GATC 9 cut(s) 70, 220, 243, 646, 852, 878, 934, 979, 1299
DriI GACNNNNNGTC 1 cut(s) 1236
Eam1105I GACNNNNNGTC 1 cut(s) 1236
EciI GGCGGA 1 cut(s) 1433
Ecl136II GAGCTC 1 cut(s) 1119
Eco130I CCWWGG 2 cut(s) 1191, 1288
Eco24I GRGCYC 2 cut(s) 1121, 1253
Eco47I GGWCC 1 cut(s) 1129
Eco47III AGCGCT 1 cut(s) 629
Eco53kI GAGCTC 1 cut(s) 1119
Eco57I CTGAAG 1 cut(s) 36
Eco88I CYCGRG 2 cut(s) 1139, 1304
EcoICRI GAGCTC 1 cut(s) 1119
EcoRI GAATTC 2 cut(s) 460, 1281
EcoRII CCWGG 1 cut(s) 1093
EcoT14I CCWWGG 2 cut(s) 1191, 1288
EcoT38I GRGCYC 2 cut(s) 1121, 1253
ErhI CCWWGG 2 cut(s) 1191, 1288
FaeI CATG 7 cut(s) 95, 272, 300, 430, 474, 680, 1107
FalI AAGNNNNNCTT 2 cut(s) 605, 637
FaqI GGGAC 1 cut(s) 877
FatI CATG 7 cut(s) 91, 268, 296, 426, 470, 676, 1103
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 2 cut(s) 1027, 1396
Fnu4HI GCNGC 1 cut(s) 301
FokI GGATG 2 cut(s) 937, 1208
FriOI GRGCYC 2 cut(s) 1121, 1253
Fsp4HI GCNGC 1 cut(s) 301
FspBI CTAG 2 cut(s) 354, 476
GlaI GCGC 1 cut(s) 629
GluI GCNGC 1 cut(s) 301
GsuI CTGGAG 2 cut(s) 752, 974
HaeII RGCGCY 1 cut(s) 631
HapII CCGG 2 cut(s) 720, 1447
HhaI GCGC 1 cut(s) 630
Hin1II CATG 7 cut(s) 95, 272, 300, 430, 474, 680, 1107
Hin6I GCGC 1 cut(s) 628
HinP1I GCGC 1 cut(s) 628
HincII GTYRAC 2 cut(s) 422, 787
HindII GTYRAC 2 cut(s) 422, 787
HinfI GANTC 6 cut(s) 63, 744, 802, 994, 1069, 1231
HpaII CCGG 2 cut(s) 720, 1447
HphI GGTGA 2 cut(s) 218, 1256
Hpy166II GTNNAC 7 cut(s) 35, 422, 518, 787, 1028, 1132, 1397
Hpy188I TCNGA 6 cut(s) 336, 406, 979, 1003, 1015, 1280
Hpy8I GTNNAC 7 cut(s) 35, 422, 518, 787, 1028, 1132, 1397
HpyCH4III ACNGT 3 cut(s) 23, 291, 926
HpyCH4IV ACGT 1 cut(s) 669
HpyCH4V TGCA 5 cut(s) 91, 1209, 1313, 1325, 1357
HpyF3I CTNAG 4 cut(s) 622, 1233, 1259, 1412
HpySE526I ACGT 1 cut(s) 669
Hsp92II CATG 7 cut(s) 95, 272, 300, 430, 474, 680, 1107
HspAI GCGC 1 cut(s) 628
Kpn2I TCCGGA 1 cut(s) 719
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 9 cut(s) 70, 220, 243, 646, 852, 878, 934, 979, 1299
LweI GCATC 2 cut(s) 1189, 1460
MaeI CTAG 2 cut(s) 354, 476
MaeII ACGT 1 cut(s) 669
MaeIII GTNAC 1 cut(s) 1262
MalI GATC 9 cut(s) 72, 222, 245, 648, 854, 880, 936, 981, 1301
MboI GATC 9 cut(s) 70, 220, 243, 646, 852, 878, 934, 979, 1299
MfeI CAATTG 1 cut(s) 1204
MflI RGATCY 1 cut(s) 878
MhlI GDGCHC 3 cut(s) 1121, 1253, 1338
MlyI GAGTC 3 cut(s) 753, 1078, 1225
MroI TCCGGA 1 cut(s) 719
MseI TTAA 4 cut(s) 440, 701, 915, 1430
MspA1I CMGCKG 1 cut(s) 56
MspI CCGG 2 cut(s) 720, 1447
MspR9I CCNGG 2 cut(s) 1095, 1448
MunI CAATTG 1 cut(s) 1204
MvaI CCWGG 1 cut(s) 1095
NciI CCSGG 1 cut(s) 1448
NdeII GATC 9 cut(s) 70, 220, 243, 646, 852, 878, 934, 979, 1299
NlaIII CATG 7 cut(s) 95, 272, 300, 430, 474, 680, 1107
NlaIV GGNNCC 1 cut(s) 333
NmuCI GTSAC 1 cut(s) 1262
NspI RCATGY 3 cut(s) 95, 680, 1107
PaeI GCATGC 1 cut(s) 1107
PaeR7I CTCGAG 1 cut(s) 1139
PciI ACATGT 1 cut(s) 676
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PfeI GAWTC 3 cut(s) 63, 802, 994
PflMI CCANNNNNTGG 2 cut(s) 323, 1192
PkrI GCNGC 1 cut(s) 302
PleI GAGTC 3 cut(s) 752, 1077, 1225
PpsI GAGTC 3 cut(s) 752, 1077, 1225
PscI ACATGT 1 cut(s) 676
PshBI ATTAAT 1 cut(s) 915
PsiI TTATAA 1 cut(s) 971
Psp124BI GAGCTC 1 cut(s) 1121
Psp6I CCWGG 1 cut(s) 1093
PspGI CCWGG 1 cut(s) 1093
PspN4I GGNNCC 1 cut(s) 333
PspPI GGNCC 1 cut(s) 1129
PsrI GAACNNNNNNTAC 2 cut(s) 789, 821
PsuI RGATCY 1 cut(s) 878
PvuII CAGCTG 1 cut(s) 56
RsaI GTAC 2 cut(s) 293, 372
RsaNI GTAC 2 cut(s) 292, 371
SacI GAGCTC 1 cut(s) 1121
SaqAI TTAA 4 cut(s) 440, 701, 915, 1430
SatI GCNGC 1 cut(s) 301
Sau3AI GATC 9 cut(s) 70, 220, 243, 646, 852, 878, 934, 979, 1299
Sau96I GGNCC 1 cut(s) 1129
SchI GAGTC 3 cut(s) 753, 1078, 1225
ScrFI CCNGG 2 cut(s) 1095, 1448
SduI GDGCHC 3 cut(s) 1121, 1253, 1338
SfaNI GCATC 2 cut(s) 1189, 1460
SfcI CTRYAG 1 cut(s) 631
Sfr274I CTCGAG 1 cut(s) 1139
SinI GGWCC 1 cut(s) 1129
SlaI CTCGAG 1 cut(s) 1139
SmlI CTYRAG 2 cut(s) 1057, 1139
SmoI CTYRAG 2 cut(s) 1057, 1139
SphI GCATGC 1 cut(s) 1107
SsiI CCGC 3 cut(s) 301, 1023, 1444
SspI AATATT 2 cut(s) 258, 1244
SspMI CTAG 2 cut(s) 354, 476
SstI GAGCTC 1 cut(s) 1121
StyD4I CCNGG 2 cut(s) 1093, 1446
StyI CCWWGG 2 cut(s) 1191, 1288
TaaI ACNGT 3 cut(s) 23, 291, 926
TaiI ACGT 1 cut(s) 672
TaqI TCGA 7 cut(s) 148, 186, 375, 609, 851, 933, 1140
TatI WGTACW 1 cut(s) 370
TauI GCSGC 1 cut(s) 303
TfiI GAWTC 3 cut(s) 63, 802, 994
Tru1I TTAA 4 cut(s) 440, 701, 915, 1430
Tru9I TTAA 4 cut(s) 440, 701, 915, 1430
TscAI CASTG 2 cut(s) 73, 931
TseFI GTSAC 1 cut(s) 1262
Tsp45I GTSAC 1 cut(s) 1262
TspDTI ATGAA 8 cut(s) 285, 512, 585, 874, 939, 1058, 1091, 1397
TspGWI ACGGA 2 cut(s) 112, 503
TspRI CASTG 2 cut(s) 73, 931
Van91I CCANNNNNTGG 2 cut(s) 323, 1192
VpaK11BI GGWCC 1 cut(s) 1129
VspI ATTAAT 1 cut(s) 915
XapI RAATTY 6 cut(s) 306, 460, 654, 1281, 1319, 1466
XceI RCATGY 3 cut(s) 95, 680, 1107
XhoI CTCGAG 1 cut(s) 1139
XmiI GTMKAC 2 cut(s) 1027, 1396
XspI CTAG 2 cut(s) 354, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.