Rroxscaffold_3G00218730

peroxisomal (S)-2-hydroxy-acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000887
Physical Location & Seq
Forward (+)
1025909 .. 1030089
4181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00218730.1

Sequence Viewer

Length: 561 bp
ATGGATATACGGAGTATGAAGAAGAAGAAGAAGAAGAAGAAGGGTGCAATCGTAGCATCGGGGCACATTGTTGGCACAATGATAATGAGGAAAAGCAAAATGCTTAAGTTGTCTATTGGATACGTAGCTGACTCTTTGAAACGTGATCGTCGTGTGTTTTACGAGAGAAGCTGTTCAAACCAATACAAGCGTCCCAAGGACAAGGTTCTCGATATCCGGTTTACTATGCCACCAAGTTTGACATTGAAAAACTTTGAAAATTTGGATCTTGGAAAGATTGATGAGACCAATGATTCTGGATTAGCATTGTATGTTGCTGGACTGTTTGCCCGCTCTCTCACTGGAAGGTTATGCATACAAGCTGGAGCTGCAGGAATAATTGTATCCAATCATGGAGCTCGGCAACTAGATTATGTCCCTGCAACTATTATGGCTTTGGAAGAGTGGGCGGTAAAGGATTGGAAATGGTATACCCAACAACAACTTGACGAGGTGTGCATTGAAGTAGTCGACTACTTACGCATCGCTGTAGATTCGACCAGTTTCTTGATTTTGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00630 ko01100 ko01110 ko01120 ko01130 ko01200 ko04146 map00630 map01100 map01110 map01120 map01130 map01200 map04146
Pfam Domains
Protein Families
FMN_dh

Protein Analysis

186

Amino Acids

21.07

Weight (kDa)

9.61

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMN_dh PF01070 116 - 149 2.2e-10 FMN-dependent dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 333
AccI GTMKAC 2 cut(s) 470, 510
AciI CCGC 2 cut(s) 331, 449
AclWI GGATC 1 cut(s) 273
AcsI RAATTY 1 cut(s) 259
AflII CTTAAG 1 cut(s) 104
AgsI TTSAA 5 cut(s) 139, 177, 247, 257, 503
AluBI AGCT 5 cut(s) 128, 171, 362, 368, 398
AluI AGCT 5 cut(s) 128, 171, 362, 368, 398
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 1 cut(s) 278
AlwI GGATC 1 cut(s) 273
ApeKI GCWGC 1 cut(s) 368
ApoI RAATTY 1 cut(s) 259
Asp700I GAANNNNTTC 1 cut(s) 172
BaeGI GKGCMC 1 cut(s) 66
BanII GRGCYC 1 cut(s) 400
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 1 cut(s) 355
BciVI GTATCC 2 cut(s) 113, 394
BcoDI GTCTC 1 cut(s) 278
BfaI CTAG 1 cut(s) 407
BfmI CTRYAG 2 cut(s) 369, 528
BfrI CTTAAG 1 cut(s) 104
BfuI GTATCC 2 cut(s) 113, 394
BisI GCNGC 1 cut(s) 369
BlsI GCNGC 1 cut(s) 370
BmsI GCATC 2 cut(s) 65, 531
BpmI CTGGAG 1 cut(s) 384
BsaAI YACGTR 1 cut(s) 124
BsaI GGTCTC 1 cut(s) 278
BsaJI CCNNGG 1 cut(s) 195
BsaWI WCCGGW 1 cut(s) 216
Bse1I ACTGG 2 cut(s) 346, 540
BseDI CCNNGG 1 cut(s) 195
BseNI ACTGG 2 cut(s) 346, 540
BseSI GKGCMC 1 cut(s) 66
BseXI GCAGC 1 cut(s) 355
BsiHKAI GWGCWC 1 cut(s) 400
BsiSI CCGG 1 cut(s) 217
BslFI GGGAC 2 cut(s) 177, 401
BsmAI GTCTC 1 cut(s) 278
BsmFI GGGAC 2 cut(s) 177, 401
Bso31I GGTCTC 1 cut(s) 278
Bsp1286I GDGCHC 2 cut(s) 66, 400
Bsp143I GATC 2 cut(s) 145, 265
BspACI CCGC 2 cut(s) 331, 449
BspMAI CTGCAG 1 cut(s) 373
BspPI GGATC 1 cut(s) 273
BspTI CTTAAG 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 278
BsrBI CCGCTC 1 cut(s) 333
BsrI ACTGG 2 cut(s) 346, 540
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 2 cut(s) 145, 265
BssNAI GTATAC 1 cut(s) 471
BssT1I CCWWGG 1 cut(s) 195
Bst1107I GTATAC 1 cut(s) 471
Bst4CI ACNGT 1 cut(s) 324
Bst6I CTCTTC 1 cut(s) 435
BstAFI CTTAAG 1 cut(s) 104
BstBAI YACGTR 1 cut(s) 124
BstC8I GCNNGC 1 cut(s) 331
BstKTI GATC 2 cut(s) 148, 268
BstMAI GTCTC 1 cut(s) 278
BstMBI GATC 2 cut(s) 145, 265
BstMWI GCNNNNNNNGC 2 cut(s) 53, 368
BstSFI CTRYAG 2 cut(s) 369, 528
BstSLI GKGCMC 1 cut(s) 66
BstSNI TACGTA 1 cut(s) 124
BstV1I GCAGC 1 cut(s) 355
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BstZ17I GTATAC 1 cut(s) 471
BsuI GTATCC 2 cut(s) 113, 394
BtgZI GCGATG 1 cut(s) 508
BtsIMutI CAGTG 1 cut(s) 339
Cac8I GCNNGC 1 cut(s) 331
CseI GACGC 1 cut(s) 179
CspCI CAANNNNNGTGG 2 cut(s) 219, 254
CviAII CATG 1 cut(s) 392
CviJI RGCY 6 cut(s) 128, 171, 362, 368, 398, 434
CviKI_1 RGCY 6 cut(s) 128, 171, 362, 368, 398, 434
DpnI GATC 2 cut(s) 147, 267
DpnII GATC 2 cut(s) 145, 265
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
Ecl136II GAGCTC 1 cut(s) 398
Eco105I TACGTA 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 400
Eco31I GGTCTC 1 cut(s) 278
Eco32I GATATC 1 cut(s) 214
Eco53kI GAGCTC 1 cut(s) 398
EcoICRI GAGCTC 1 cut(s) 398
EcoRV GATATC 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 356
EcoT38I GRGCYC 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 1 cut(s) 395
FaqI GGGAC 2 cut(s) 177, 401
FatI CATG 1 cut(s) 391
FauI CCCGC 1 cut(s) 338
FblI GTMKAC 2 cut(s) 470, 510
Fnu4HI GCNGC 1 cut(s) 369
FriOI GRGCYC 1 cut(s) 400
Fsp4HI GCNGC 1 cut(s) 369
FspBI CTAG 1 cut(s) 407
GluI GCNGC 1 cut(s) 369
GsuI CTGGAG 1 cut(s) 384
HapII CCGG 1 cut(s) 217
HgaI GACGC 1 cut(s) 179
Hin1II CATG 1 cut(s) 395
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HinfI GANTC 3 cut(s) 131, 293, 533
HpaII CCGG 1 cut(s) 217
Hpy166II GTNNAC 3 cut(s) 222, 471, 511
Hpy188III TCNNGA 3 cut(s) 209, 297, 547
Hpy8I GTNNAC 3 cut(s) 222, 471, 511
Hpy99I CGWCG 1 cut(s) 153
HpyAV CCTTC 2 cut(s) 34, 339
HpyCH4III ACNGT 1 cut(s) 324
HpyCH4IV ACGT 2 cut(s) 123, 142
HpyCH4V TGCA 5 cut(s) 47, 354, 371, 422, 498
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 368
HpySE526I ACGT 2 cut(s) 123, 142
Hsp92II CATG 1 cut(s) 395
Kzo9I GATC 2 cut(s) 145, 265
LmnI GCTCC 2 cut(s) 365, 395
LpnPI CCDG 8 cut(s) 230, 282, 303, 327, 348, 357, 432, 553
Lsp1109I GCAGC 1 cut(s) 355
LweI GCATC 2 cut(s) 65, 531
MaeI CTAG 1 cut(s) 407
MaeII ACGT 2 cut(s) 123, 142
MalI GATC 2 cut(s) 147, 267
MbiI CCGCTC 1 cut(s) 333
MboI GATC 2 cut(s) 145, 265
MboII GAAGA 8 cut(s) 31, 34, 37, 40, 43, 46, 49, 452
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 2 cut(s) 66, 400
MluCI AATT 2 cut(s) 259, 378
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 2 cut(s) 81, 484
Mph1103I ATGCAT 1 cut(s) 356
MroXI GAANNNNTTC 1 cut(s) 172
MseI TTAA 1 cut(s) 105
MspCI CTTAAG 1 cut(s) 104
MspI CCGG 1 cut(s) 217
MwoI GCNNNNNNNGC 2 cut(s) 53, 368
NdeII GATC 2 cut(s) 145, 265
NlaIII CATG 1 cut(s) 395
NmeAIII GCCGAG 1 cut(s) 379
NsiI ATGCAT 1 cut(s) 356
PcsI WCGNNNNNNNCGW 1 cut(s) 148
PdmI GAANNNNTTC 1 cut(s) 172
PfeI GAWTC 2 cut(s) 293, 533
PkrI GCNGC 1 cut(s) 370
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
Ppu21I YACGTR 1 cut(s) 124
Psp124BI GAGCTC 1 cut(s) 400
PstI CTGCAG 1 cut(s) 373
PsuI RGATCY 1 cut(s) 265
SacI GAGCTC 1 cut(s) 400
SalI GTCGAC 1 cut(s) 509
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 1 cut(s) 369
Sau3AI GATC 2 cut(s) 145, 265
SchI GAGTC 1 cut(s) 125
SduI GDGCHC 2 cut(s) 66, 400
SfaNI GCATC 2 cut(s) 65, 531
SfcI CTRYAG 2 cut(s) 369, 528
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
SnaBI TACGTA 1 cut(s) 124
Sse9I AATT 2 cut(s) 259, 378
SsiI CCGC 2 cut(s) 331, 449
SspMI CTAG 1 cut(s) 407
SstI GAGCTC 1 cut(s) 400
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 1 cut(s) 324
TaiI ACGT 2 cut(s) 126, 145
TaqI TCGA 3 cut(s) 210, 510, 536
TasI AATT 2 cut(s) 259, 378
TfiI GAWTC 2 cut(s) 293, 533
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 1 cut(s) 346
TseI GCWGC 1 cut(s) 368
TspDTI ATGAA 1 cut(s) 32
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 1 cut(s) 346
Vha464I CTTAAG 1 cut(s) 104
XapI RAATTY 1 cut(s) 259
XmiI GTMKAC 2 cut(s) 470, 510
XmnI GAANNNNTTC 1 cut(s) 172
XspI CTAG 1 cut(s) 407
Zsp2I ATGCAT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.