Rh5AG529300

peroxisomal (S)-2-hydroxy-acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000887
Physical Location & Seq
Forward (+)
90800015 .. 90856386
56372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG529300.1

Sequence Viewer

Length: 552 bp
ATGGAATCCTTCGCCCTCTTCCTCTTCCCGCTCAACCCTTCATTCAGATCCAGTCCCTGGAAGAATTGTCTCCCACCGCTCCAGCCTCTGGTTTTGGGGTTAAGGTCTGTTTTAGTGCAAAGTGTTTTAGAGTTGATGATGCGTGTGTGCTCGAGTGCGTGTCATGGACCCATCACCATTTCTCACTTGCTCAAAACTTCAAAAGGACACTGTCCGCTATGGCTAATTCTAGCATTGACGAAGTTGCCTCAACTGGACCTGGTATTCGCTTGTTCCACCTCTATGTTTACTATGTCACCAACTTTGACATTGAAAAACTTTGAAAATTTGGATCTTGGAAAGATTGATGAGACCAATAATTCTGGATTAGCATCATATGTTGCTGGACTATTTGACCGCTCTTTTAGTGGGAAGGTCGTCAAAGCTGCAGAGGGCCAAATTCCTGTTTTTCTGGATGGTGGAGTGAGGCAAGGCACATATGTTTTTAAAGCTTTGGATCTTGGAGCATCAAGTGTAATTTATAAATACTATACCACCTCCGGTCCTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00630 ko01100 ko01110 ko01120 ko01130 ko01200 ko04146 map00630 map01100 map01110 map01120 map01130 map01200 map04146
Pfam Domains
Protein Families
FMN_dh

Protein Analysis

183

Amino Acids

19.95

Weight (kDa)

8.84

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMN_dh PF01070 138 - 173 3e-08 FMN-dependent dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 522
AccB7I CCANNNNNTGG 2 cut(s) 57, 88
AccBSI CCGCTC 3 cut(s) 31, 79, 399
AciI CCGC 4 cut(s) 29, 77, 215, 397
AclWI GGATC 3 cut(s) 42, 339, 504
AcsI RAATTY 2 cut(s) 325, 438
AfiI CCNNNNNNNGG 2 cut(s) 57, 88
AgsI TTSAA 3 cut(s) 201, 313, 323
AjnI CCWGG 2 cut(s) 56, 258
AluBI AGCT 2 cut(s) 425, 491
AluI AGCT 2 cut(s) 425, 491
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 2 cut(s) 74, 344
AlwI GGATC 3 cut(s) 42, 339, 504
AlwNI CAGNNNCTG 2 cut(s) 57, 88
Ama87I CYCGRG 1 cut(s) 151
AoxI GGCC 1 cut(s) 433
ApeKI GCWGC 1 cut(s) 425
ApoI RAATTY 2 cut(s) 325, 438
ArsI GACNNNNNNTTYG 2 cut(s) 248, 280
AspS9I GGNCC 4 cut(s) 167, 256, 433, 542
AsuHPI GGTGA 2 cut(s) 166, 288
AvaI CYCGRG 1 cut(s) 151
AvaII GGWCC 3 cut(s) 167, 256, 542
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 1 cut(s) 412
BccI CCATC 2 cut(s) 179, 449
BciT130I CCWGG 2 cut(s) 58, 260
BcoDI GTCTC 2 cut(s) 74, 344
BfaI CTAG 1 cut(s) 230
BfmI CTRYAG 1 cut(s) 426
BisI GCNGC 1 cut(s) 426
BlsI GCNGC 1 cut(s) 427
Bme1390I CCNGG 2 cut(s) 58, 260
Bme18I GGWCC 3 cut(s) 167, 256, 542
BmeT110I CYCGRG 1 cut(s) 151
BmgT120I GGNCC 4 cut(s) 167, 256, 433, 542
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 2 cut(s) 58, 260
BmsI GCATC 3 cut(s) 129, 380, 515
BpmI CTGGAG 1 cut(s) 65
BsaBI GATNNNNATC 1 cut(s) 370
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 1 cut(s) 56
BsaWI WCCGGW 1 cut(s) 539
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 88
Bse1I ACTGG 2 cut(s) 51, 258
Bse8I GATNNNNATC 1 cut(s) 370
BseBI CCWGG 2 cut(s) 58, 260
BseDI CCNNGG 1 cut(s) 56
BseGI GGATG 1 cut(s) 460
BseJI GATNNNNATC 1 cut(s) 370
BseLI CCNNNNNNNGG 2 cut(s) 57, 88
BseNI ACTGG 2 cut(s) 51, 258
BseXI GCAGC 1 cut(s) 412
BshFI GGCC 1 cut(s) 435
BsiHKAI GWGCWC 1 cut(s) 152
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 1 cut(s) 540
BslFI GGGAC 1 cut(s) 39
BslI CCNNNNNNNGG 2 cut(s) 57, 88
BsmAI GTCTC 2 cut(s) 74, 344
BsmFI GGGAC 1 cut(s) 39
BsnI GGCC 1 cut(s) 435
Bso31I GGTCTC 1 cut(s) 344
BsoBI CYCGRG 1 cut(s) 151
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 3 cut(s) 47, 331, 496
BspACI CCGC 4 cut(s) 29, 77, 215, 397
BspANI GGCC 1 cut(s) 435
BspLI GGNNCC 1 cut(s) 169
BspMAI CTGCAG 1 cut(s) 430
BspPI GGATC 3 cut(s) 42, 339, 504
BspTNI GGTCTC 1 cut(s) 344
BsrBI CCGCTC 3 cut(s) 31, 79, 399
BsrI ACTGG 2 cut(s) 51, 258
BssECI CCNNGG 1 cut(s) 56
BssMI GATC 3 cut(s) 47, 331, 496
Bst2UI CCWGG 2 cut(s) 58, 260
Bst4CI ACNGT 1 cut(s) 212
Bst6I CTCTTC 2 cut(s) 23, 29
BstF5I GGATG 1 cut(s) 460
BstKTI GATC 3 cut(s) 50, 334, 499
BstMAI GTCTC 2 cut(s) 74, 344
BstMBI GATC 3 cut(s) 47, 331, 496
BstNI CCWGG 2 cut(s) 58, 260
BstSCI CCNGG 2 cut(s) 56, 258
BstSFI CTRYAG 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 412
BstX2I RGATCY 3 cut(s) 47, 331, 496
BstYI RGATCY 3 cut(s) 47, 331, 496
BsuRI GGCC 1 cut(s) 435
BtsCI GGATG 1 cut(s) 460
BtsIMutI CAGTG 1 cut(s) 208
CaiI CAGNNNCTG 2 cut(s) 57, 88
Cfr13I GGNCC 4 cut(s) 167, 256, 433, 542
CsiI ACCWGGT 1 cut(s) 258
CviAII CATG 1 cut(s) 164
CviJI RGCY 5 cut(s) 85, 223, 425, 435, 491
CviKI_1 RGCY 5 cut(s) 85, 223, 425, 435, 491
DpnI GATC 3 cut(s) 49, 333, 498
DpnII GATC 3 cut(s) 47, 331, 496
DraI TTTAAA 1 cut(s) 487
Eam1104I CTCTTC 2 cut(s) 23, 29
EarI CTCTTC 2 cut(s) 23, 29
Eco31I GGTCTC 1 cut(s) 344
Eco47I GGWCC 3 cut(s) 167, 256, 542
Eco88I CYCGRG 1 cut(s) 151
EcoRII CCWGG 2 cut(s) 56, 258
FaeI CATG 1 cut(s) 167
FaqI GGGAC 1 cut(s) 39
FatI CATG 1 cut(s) 163
FauI CCCGC 1 cut(s) 36
FauNDI CATATG 2 cut(s) 376, 478
Fnu4HI GCNGC 1 cut(s) 426
FokI GGATG 1 cut(s) 467
Fsp4HI GCNGC 1 cut(s) 426
FspBI CTAG 1 cut(s) 230
GluI GCNGC 1 cut(s) 426
GsuI CTGGAG 1 cut(s) 65
HaeIII GGCC 1 cut(s) 435
HapII CCGG 1 cut(s) 540
Hin1II CATG 1 cut(s) 167
HindIII AAGCTT 1 cut(s) 489
HinfI GANTC 1 cut(s) 5
HpaII CCGG 1 cut(s) 540
HphI GGTGA 2 cut(s) 166, 288
Hpy166II GTNNAC 1 cut(s) 288
Hpy188I TCNGA 1 cut(s) 47
Hpy188III TCNNGA 2 cut(s) 363, 452
Hpy8I GTNNAC 1 cut(s) 288
HpyAV CCTTC 3 cut(s) 19, 48, 406
HpyCH4III ACNGT 1 cut(s) 212
HpyCH4V TGCA 2 cut(s) 118, 428
Hsp92II CATG 1 cut(s) 167
Kzo9I GATC 3 cut(s) 47, 331, 496
LmnI GCTCC 2 cut(s) 84, 503
Lsp1109I GCAGC 1 cut(s) 412
LweI GCATC 3 cut(s) 129, 380, 515
MabI ACCWGGT 1 cut(s) 258
MaeI CTAG 1 cut(s) 230
MaeIII GTNAC 1 cut(s) 294
MalI GATC 3 cut(s) 49, 333, 498
MbiI CCGCTC 3 cut(s) 31, 79, 399
MboI GATC 3 cut(s) 47, 331, 496
MboII GAAGA 3 cut(s) 10, 16, 73
MflI RGATCY 3 cut(s) 47, 331, 496
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 6 cut(s) 64, 225, 325, 358, 438, 516
MnlI CCTC 8 cut(s) 26, 32, 96, 258, 289, 424, 459, 547
MseI TTAA 2 cut(s) 101, 486
MslI CAYNNNNRTG 1 cut(s) 281
MspI CCGG 1 cut(s) 540
MspR9I CCNGG 2 cut(s) 58, 260
MvaI CCWGG 2 cut(s) 58, 260
NdeI CATATG 2 cut(s) 376, 478
NdeII GATC 3 cut(s) 47, 331, 496
NlaIII CATG 1 cut(s) 167
NlaIV GGNNCC 1 cut(s) 169
NmuCI GTSAC 1 cut(s) 294
PaeR7I CTCGAG 1 cut(s) 151
PfeI GAWTC 1 cut(s) 5
PflFI GACNNNGTC 1 cut(s) 210
PflMI CCANNNNNTGG 2 cut(s) 57, 88
PkrI GCNGC 1 cut(s) 427
PsiI TTATAA 1 cut(s) 522
Psp6I CCWGG 2 cut(s) 56, 258
PspGI CCWGG 2 cut(s) 56, 258
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 4 cut(s) 167, 256, 433, 542
PspXI VCTCGAGB 1 cut(s) 151
PstI CTGCAG 1 cut(s) 430
PstNI CAGNNNCTG 2 cut(s) 57, 88
PsuI RGATCY 3 cut(s) 47, 331, 496
PsyI GACNNNGTC 1 cut(s) 210
RseI CAYNNNNRTG 1 cut(s) 281
SaqAI TTAA 2 cut(s) 101, 486
SatI GCNGC 1 cut(s) 426
Sau3AI GATC 3 cut(s) 47, 331, 496
Sau96I GGNCC 4 cut(s) 167, 256, 433, 542
ScrFI CCNGG 2 cut(s) 58, 260
SduI GDGCHC 1 cut(s) 152
SetI ASST 7 cut(s) 107, 261, 281, 417, 427, 493, 539
SexAI ACCWGGT 1 cut(s) 258
SfaNI GCATC 3 cut(s) 129, 380, 515
SfcI CTRYAG 1 cut(s) 426
Sfr274I CTCGAG 1 cut(s) 151
SinI GGWCC 3 cut(s) 167, 256, 542
SlaI CTCGAG 1 cut(s) 151
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
Sse9I AATT 6 cut(s) 64, 225, 325, 358, 438, 516
SsiI CCGC 4 cut(s) 29, 77, 215, 397
SspMI CTAG 1 cut(s) 230
StyD4I CCNGG 2 cut(s) 56, 258
TaaI ACNGT 1 cut(s) 212
TaqI TCGA 1 cut(s) 152
TasI AATT 6 cut(s) 64, 225, 325, 358, 438, 516
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 2 cut(s) 101, 486
Tru9I TTAA 2 cut(s) 101, 486
TscAI CASTG 1 cut(s) 215
TseFI GTSAC 1 cut(s) 294
TseI GCWGC 1 cut(s) 425
Tsp45I GTSAC 1 cut(s) 294
TspDTI ATGAA 1 cut(s) 30
TspRI CASTG 1 cut(s) 215
Tth111I GACNNNGTC 1 cut(s) 210
Van91I CCANNNNNTGG 2 cut(s) 57, 88
VpaK11BI GGWCC 3 cut(s) 167, 256, 542
XapI RAATTY 2 cut(s) 325, 438
XhoI CTCGAG 1 cut(s) 151
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.