Rorug05G0253800

peroxisomal (S)-2-hydroxy-acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000887
Physical Location & Seq
Reverse (-)
27948124 .. 27949855
1732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0253800.1

Sequence Viewer

Length: 1041 bp
ATGTTCTGGGCGCCCCACCATACCCTACCTCCAGAATTGCTTTGGACACCCACCTATGACAAGCACCGCCATCGGTTGCGCCTCTTTGTTGTCGACAAACTCCCCCGTAGCTATCCGTGCCGACACGCCTCTTTGTTGCCTATCTTCAGACCCAAGATCTGCAGCGCTGGAAACATGGCTCTGCCACAAAAAAAATCCGGCCAGAATCTCTGGCCTCAGCCACCAGTTAGCATCGATCACAACCAAGACCTTGAAAGCTCGGCGGCGGCAAAGTCATCCATTCCGGCTTCCAAGTGGGGGATCTCGGTTGAAAATTCATCGACCACAATCGACGTTTGCTTGCCTCCAGAAGATGGGTTGGGGCAGAGAGTACTCCTCTGCTTGACGTTGGCGGCTAGGTTTTCTGACGACATATCCCTATTCAGGTACCCAAACGATGTTAATGATCGTATCTGGTATCCTGCTTTCAGGACTGATTGGGATGAATTAAGTACTTCGTCTGTTATCGACATGGATCAAGCCGCTTACCAACTCCCATCTGATGTCATGAGAACCGCTGCCACGCCAAAATATCCAAGCAATATCCTGGATTTTTCCTGGACGCCTCCCGTCGGTAAAAATGCATCATATTATATTTATATGCACTTTGCAGAAGTTGAAGAGTTCAGCGCCACCTGGTCTAGAAAGCTCACTATTACCATGAATGGAACGCTTATTTTTAAGCCATTTGATCCACCTTACTTGAGCACAAGAACGATTGACACTACGTTTACTGCGGAAGGAGGAACGTATTATTTTTCAATATCTACACCGCCGACAATGTATCAACCGATTCTGAATGCCTTTGAGAGTTATATGGCAAAAGAATTTTTAGTATCAGAAACAAACCAGCAAGACGTTGAAGCAATTACGAACCTCAAGTCAACTTATAAAATCATTAAGAATTGGCAAGGAGATCCGTGCAACCCTCAAGTTTACCTGTGGGAAGGTGTAAAGTGTAGCTATTCTGACAATGACTCCTCACGAATCATATCCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00630 ko01100 ko01110 ko01120 ko01130 ko01200 ko04146 map00630 map01100 map01110 map01120 map01130 map01200 map04146
Pfam Domains
Protein Families
FMN_dh

Protein Analysis

346

Amino Acids

39.4

Weight (kDa)

5.82

Isoelectric Point (pI)

41.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 134 - 286 1.6e-34 Malectin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 930
Acc65I GGTACC 1 cut(s) 426
AccB1I GGYRCC 2 cut(s) 10, 426
AccB7I CCANNNNNTGG 1 cut(s) 353
AccI GTMKAC 1 cut(s) 93
AciI CCGC 8 cut(s) 67, 263, 266, 392, 522, 555, 776, 812
AclWI GGATC 4 cut(s) 308, 522, 725, 950
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 2 cut(s) 313, 866
AcuI CTGAAG 1 cut(s) 130
AcyI GRCGYC 2 cut(s) 11, 602
AfaI GTAC 3 cut(s) 372, 428, 493
AfeI AGCGCT 1 cut(s) 166
AfiI CCNNNNNNNGG 4 cut(s) 297, 353, 423, 611
AgsI TTSAA 5 cut(s) 254, 311, 659, 801, 902
AjnI CCWGG 3 cut(s) 585, 596, 674
AluBI AGCT 4 cut(s) 111, 258, 688, 1002
AluI AGCT 4 cut(s) 111, 258, 688, 1002
Alw21I GWGCWC 1 cut(s) 749
AlwI GGATC 4 cut(s) 308, 522, 725, 950
Aor51HI AGCGCT 1 cut(s) 166
AoxI GGCC 2 cut(s) 199, 212
ApeKI GCWGC 2 cut(s) 162, 557
ApoI RAATTY 2 cut(s) 313, 866
ArsI GACNNNNNNTTYG 2 cut(s) 905, 937
Asp718I GGTACC 1 cut(s) 426
AspLEI GCGC 4 cut(s) 13, 81, 167, 671
BanI GGYRCC 2 cut(s) 10, 426
Bbv12I GWGCWC 1 cut(s) 749
BbvCI CCTCAGC 1 cut(s) 216
BbvI GCAGC 2 cut(s) 174, 544
BccI CCATC 3 cut(s) 78, 347, 544
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BciT130I CCWGG 3 cut(s) 587, 598, 676
BciVI GTATCC 1 cut(s) 468
BfaI CTAG 2 cut(s) 396, 681
BfmI CTRYAG 1 cut(s) 160
BfoI RGCGCY 3 cut(s) 14, 168, 672
BfuI GTATCC 1 cut(s) 468
BglII AGATCT 1 cut(s) 156
BisI GCNGC 6 cut(s) 163, 264, 267, 393, 522, 558
BlsI GCNGC 6 cut(s) 164, 265, 268, 394, 523, 559
BmcAI AGTACT 2 cut(s) 372, 493
Bme1390I CCNGG 3 cut(s) 587, 598, 676
BmiI GGNNCC 2 cut(s) 12, 428
BmrFI CCNGG 3 cut(s) 587, 598, 676
BmsI GCATC 2 cut(s) 240, 632
BplI GAGNNNNNCTC 2 cut(s) 360, 392
BpmI CTGGAG 2 cut(s) 15, 330
Bpu10I CCTNAGC 1 cut(s) 216
BpuEI CTTGAG 3 cut(s) 763, 902, 954
Bsa29I ATCGAT 1 cut(s) 234
BsaHI GRCGYC 2 cut(s) 11, 602
BsaXI ACNNNNNCTCC 4 cut(s) 13, 43, 1001, 1031
Bsc4I CCNNNNNNNGG 4 cut(s) 297, 353, 423, 611
Bse1I ACTGG 1 cut(s) 224
BseBI CCWGG 3 cut(s) 587, 598, 676
BseCI ATCGAT 1 cut(s) 234
BseGI GGATG 2 cut(s) 275, 487
BseLI CCNNNNNNNGG 4 cut(s) 297, 353, 423, 611
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 1 cut(s) 224
BseRI GAGGAG 2 cut(s) 365, 1009
BseXI GCAGC 2 cut(s) 174, 544
BshFI GGCC 2 cut(s) 201, 214
BshNI GGYRCC 2 cut(s) 10, 426
BshVI ATCGAT 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 749
BsiSI CCGG 2 cut(s) 198, 284
BslI CCNNNNNNNGG 4 cut(s) 297, 353, 423, 611
BsmI GAATGC 1 cut(s) 844
BsnI GGCC 2 cut(s) 201, 214
Bsp1286I GDGCHC 1 cut(s) 749
Bsp143I GATC 7 cut(s) 156, 235, 300, 445, 514, 730, 955
BspACI CCGC 8 cut(s) 67, 263, 266, 392, 522, 555, 776, 812
BspANI GGCC 2 cut(s) 201, 214
BspCNI CTCAG 1 cut(s) 229
BspDI ATCGAT 1 cut(s) 234
BspHI TCATGA 1 cut(s) 546
BspLI GGNNCC 2 cut(s) 12, 428
BspMAI CTGCAG 1 cut(s) 164
BspPI GGATC 4 cut(s) 308, 522, 725, 950
BspT107I GGYRCC 2 cut(s) 10, 426
BsrI ACTGG 1 cut(s) 224
BssMI GATC 7 cut(s) 156, 235, 300, 445, 514, 730, 955
BssNI GRCGYC 2 cut(s) 11, 602
Bst2UI CCWGG 3 cut(s) 587, 598, 676
Bst6I CTCTTC 1 cut(s) 654
BstACI GRCGYC 2 cut(s) 11, 602
BstC8I GCNNGC 1 cut(s) 341
BstDEI CTNAG 1 cut(s) 216
BstF5I GGATG 2 cut(s) 275, 487
BstH2I RGCGCY 3 cut(s) 14, 168, 672
BstHHI GCGC 4 cut(s) 13, 81, 167, 671
BstKTI GATC 7 cut(s) 159, 238, 303, 448, 517, 733, 958
BstMBI GATC 7 cut(s) 156, 235, 300, 445, 514, 730, 955
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstNI CCWGG 3 cut(s) 587, 598, 676
BstSCI CCNGG 3 cut(s) 585, 596, 674
BstSFI CTRYAG 1 cut(s) 160
BstV1I GCAGC 2 cut(s) 174, 544
BstX2I RGATCY 3 cut(s) 156, 300, 955
BstYI RGATCY 3 cut(s) 156, 300, 955
Bsu15I ATCGAT 1 cut(s) 234
BsuI GTATCC 1 cut(s) 468
BsuRI GGCC 2 cut(s) 201, 214
BsuTUI ATCGAT 1 cut(s) 234
BtsCI GGATG 2 cut(s) 275, 487
Cac8I GCNNGC 1 cut(s) 341
CciI TCATGA 1 cut(s) 546
CfoI GCGC 4 cut(s) 13, 81, 167, 671
ClaI ATCGAT 1 cut(s) 234
CseI GACGC 1 cut(s) 610
CsiI ACCWGGT 1 cut(s) 674
Csp6I GTAC 3 cut(s) 371, 427, 492
CspCI CAANNNNNGTGG 2 cut(s) 723, 758
CviAII CATG 4 cut(s) 175, 511, 547, 700
CviQI GTAC 3 cut(s) 371, 427, 492
DdeI CTNAG 1 cut(s) 216
DinI GGCGCC 1 cut(s) 12
DpnI GATC 7 cut(s) 158, 237, 302, 447, 516, 732, 957
DpnII GATC 7 cut(s) 156, 235, 300, 445, 514, 730, 955
EaeI YGGCCR 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 654
EarI CTCTTC 1 cut(s) 654
Eco47III AGCGCT 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 130
EcoRII CCWGG 3 cut(s) 585, 596, 674
EcoT22I ATGCAT 1 cut(s) 625
EgeI GGCGCC 1 cut(s) 12
EheI GGCGCC 1 cut(s) 12
FaeI CATG 4 cut(s) 178, 514, 550, 703
FatI CATG 4 cut(s) 174, 510, 546, 699
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 6 cut(s) 163, 264, 267, 393, 522, 558
FokI GGATG 2 cut(s) 262, 494
Fsp4HI GCNGC 6 cut(s) 163, 264, 267, 393, 522, 558
FspBI CTAG 2 cut(s) 396, 681
GlaI GCGC 4 cut(s) 12, 80, 166, 670
GluI GCNGC 6 cut(s) 163, 264, 267, 393, 522, 558
GsuI CTGGAG 2 cut(s) 15, 330
HaeII RGCGCY 3 cut(s) 14, 168, 672
HaeIII GGCC 2 cut(s) 201, 214
HapII CCGG 2 cut(s) 198, 284
HgaI GACGC 1 cut(s) 610
HhaI GCGC 4 cut(s) 13, 81, 167, 671
Hin1I GRCGYC 2 cut(s) 11, 602
Hin1II CATG 4 cut(s) 178, 514, 550, 703
Hin6I GCGC 4 cut(s) 11, 79, 165, 669
HinP1I GCGC 4 cut(s) 11, 79, 165, 669
HincII GTYRAC 2 cut(s) 94, 924
HindII GTYRAC 2 cut(s) 94, 924
HinfI GANTC 4 cut(s) 205, 832, 1016, 1026
HpaII CCGG 2 cut(s) 198, 284
Hpy166II GTNNAC 4 cut(s) 94, 771, 924, 976
Hpy188I TCNGA 6 cut(s) 149, 406, 541, 837, 880, 1009
Hpy188III TCNNGA 6 cut(s) 32, 347, 469, 547, 681, 1023
Hpy8I GTNNAC 4 cut(s) 94, 771, 924, 976
Hpy99I CGWCG 2 cut(s) 335, 614
HpyAV CCTTC 2 cut(s) 773, 980
HpyCH4IV ACGT 5 cut(s) 333, 386, 767, 788, 897
HpyCH4V TGCA 5 cut(s) 162, 623, 643, 650, 963
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 1 cut(s) 216
HpySE526I ACGT 5 cut(s) 333, 386, 767, 788, 897
Hsp92I GRCGYC 2 cut(s) 11, 602
Hsp92II CATG 4 cut(s) 178, 514, 550, 703
HspAI GCGC 4 cut(s) 11, 79, 165, 669
KasI GGCGCC 1 cut(s) 10
KpnI GGTACC 1 cut(s) 430
Kzo9I GATC 7 cut(s) 156, 235, 300, 445, 514, 730, 955
Lsp1109I GCAGC 2 cut(s) 174, 544
LweI GCATC 2 cut(s) 240, 632
MabI ACCWGGT 1 cut(s) 674
MaeI CTAG 2 cut(s) 396, 681
MaeII ACGT 5 cut(s) 333, 386, 767, 788, 897
MalI GATC 7 cut(s) 158, 237, 302, 447, 516, 732, 957
MboI GATC 7 cut(s) 156, 235, 300, 445, 514, 730, 955
MboII GAAGA 3 cut(s) 136, 362, 671
MflI RGATCY 3 cut(s) 156, 300, 955
MhlI GDGCHC 1 cut(s) 749
MluCI AATT 6 cut(s) 35, 313, 485, 866, 906, 943
Mly113I GGCGCC 1 cut(s) 11
MlyI GAGTC 1 cut(s) 1010
Mph1103I ATGCAT 1 cut(s) 625
MseI TTAA 4 cut(s) 441, 488, 720, 939
MspA1I CMGCKG 1 cut(s) 557
MspI CCGG 2 cut(s) 198, 284
MspR9I CCNGG 3 cut(s) 587, 598, 676
Mva1269I GAATGC 1 cut(s) 844
MvaI CCWGG 3 cut(s) 587, 598, 676
MwoI GCNNNNNNNGC 1 cut(s) 117
NarI GGCGCC 1 cut(s) 11
NdeII GATC 7 cut(s) 156, 235, 300, 445, 514, 730, 955
NlaIII CATG 4 cut(s) 178, 514, 550, 703
NlaIV GGNNCC 2 cut(s) 12, 428
NmeAIII GCCGAG 1 cut(s) 239
NsiI ATGCAT 1 cut(s) 625
PagI TCATGA 1 cut(s) 546
PctI GAATGC 1 cut(s) 844
PfeI GAWTC 3 cut(s) 205, 832, 1026
PflMI CCANNNNNTGG 1 cut(s) 353
PfoI TCCNGGA 2 cut(s) 585, 596
PkrI GCNGC 6 cut(s) 164, 265, 268, 394, 523, 559
PleI GAGTC 1 cut(s) 1010
PluTI GGCGCC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 1010
PsiI TTATAA 1 cut(s) 930
Psp6I CCWGG 3 cut(s) 585, 596, 674
PspGI CCWGG 3 cut(s) 585, 596, 674
PspN4I GGNNCC 2 cut(s) 12, 428
PstI CTGCAG 1 cut(s) 164
PsuI RGATCY 3 cut(s) 156, 300, 955
RsaI GTAC 3 cut(s) 372, 428, 493
RsaNI GTAC 3 cut(s) 371, 427, 492
SalI GTCGAC 1 cut(s) 92
SaqAI TTAA 4 cut(s) 441, 488, 720, 939
SatI GCNGC 6 cut(s) 163, 264, 267, 393, 522, 558
Sau3AI GATC 7 cut(s) 156, 235, 300, 445, 514, 730, 955
ScaI AGTACT 2 cut(s) 372, 493
SchI GAGTC 1 cut(s) 1010
ScrFI CCNGG 3 cut(s) 587, 598, 676
SduI GDGCHC 1 cut(s) 749
SexAI ACCWGGT 1 cut(s) 674
SfaNI GCATC 2 cut(s) 240, 632
SfcI CTRYAG 1 cut(s) 160
SfoI GGCGCC 1 cut(s) 12
SmlI CTYRAG 3 cut(s) 742, 917, 969
SmoI CTYRAG 3 cut(s) 742, 917, 969
Sse9I AATT 6 cut(s) 35, 313, 485, 866, 906, 943
SsiI CCGC 8 cut(s) 67, 263, 266, 392, 522, 555, 776, 812
SspDI GGCGCC 1 cut(s) 10
SspMI CTAG 2 cut(s) 396, 681
StyD4I CCNGG 3 cut(s) 585, 596, 674
TaiI ACGT 5 cut(s) 336, 389, 770, 791, 900
TaqI TCGA 5 cut(s) 93, 234, 320, 330, 507
TasI AATT 6 cut(s) 35, 313, 485, 866, 906, 943
TatI WGTACW 2 cut(s) 370, 491
TauI GCSGC 4 cut(s) 266, 269, 395, 524
TfiI GAWTC 3 cut(s) 205, 832, 1026
Tru1I TTAA 4 cut(s) 441, 488, 720, 939
Tru9I TTAA 4 cut(s) 441, 488, 720, 939
TseI GCWGC 2 cut(s) 162, 557
TspDTI ATGAA 3 cut(s) 306, 498, 716
TspGWI ACGGA 2 cut(s) 105, 948
Van91I CCANNNNNTGG 1 cut(s) 353
XapI RAATTY 2 cut(s) 313, 866
XbaI TCTAGA 1 cut(s) 680
XcmI CCANNNNNNNNNTGG 1 cut(s) 39
XmiI GTMKAC 1 cut(s) 93
XspI CTAG 2 cut(s) 396, 681
ZrmI AGTACT 2 cut(s) 372, 493
Zsp2I ATGCAT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.