AT3G21950

carboxyl

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000342
Physical Location & Seq
Forward (+)
7734219 .. 7736493
2275 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G21950.1

Sequence Viewer

Length: 1107 bp
ATGGGTGTTGTTAGCATGAAGGGTGGAGATGGAGAACACAGTTATGCAAATAATTCCGAGGCGCAGAAAAGTATTACTTCTGATGCAAAACCGGAGGTGATGAAAAGCGTAAACGAAATGATAGTGAAAATGGATTTTCCTGGTTGCATCAAAGTGGCTGACTTGGGATGCTCTTCCGGAGAAAATACATTCTTGGTTATGTCCGAAATCGTTAACACAATCATTACAACATACCAACAAAATGGTCAAAATCTACCGGAGATCGATTGTTGCCTGAACGATCTTCCAGAGAACGACTTCAACACAACTTTCAAGTTGATCCCTTCCTTCCACGAGAAGCTGAAGATGAATGTTAAAGGAAATTGCTATGTCTCAGGATGCCCAGGCTCATTCTACACAAGGCTTTTCCCAAGCAAATCTCTTCATTTTGTCCATTCATCTTTTTGTCTCCATTGGCTTTCTAAAGTTCCTGATGGGCTTGAGGAAAATAAGAAGAACGTGTATCTTAGAAGTCCATGCCCTCCAAATTTGTACGAGAGTTATTGGAATCAGTTTAAGAAAGATTTCTCAATGTTTCTAAGAATGCGTGCGGAAGAGACAATGCCTAGTGGACGCATGGCTCTCACTTTAGTTGGCAGAAAAACTTTGGACCCTTTATCCAAAGAGTGTTTCAAAGATTGGTCGTTGGTTTCTGATTCCCTCCTCGATTTGGTGTCTGAGGGAGTTGTGAAGGAATCAGATTTGGAATCTTTCAACTTGCCGTATTACAGTCCTGATGAAAGTGAGGTGAAGGAAGTTATCGAGAACGAGGGCTCATTCGAGATCAAAAATTTTGAGACAATATTTGGACTTCTCTTTTCATACAAAACTGGTCATAGTGAAGTGAAAGATGATGATGATGATGTGGACCATTCACGTCGATTTGAAGTGGTTAAAACGAGGGCTAATATGACAAGATCCATCATTGAACCGATGCTTGTTGCTCATTTTGGAGAAGCCATTATTGATCGGCTCTTTGACAAGTATATATATCATGCATGTCAACGTTACGATACCTTGCGCAACAAACCAACCGTTAATTTCTTTGTTTCGTTGACTAGGAAGTGA

Protein Analysis

368

Amino Acids

42.01

Weight (kDa)

5.41

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 36 - 367 9.5e-119 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14060 AT2G14060 AT3G11480 AT3G21950 AT3G21950 AT5G04370 AT5G04370 AT5G04370 AT5G04380 AT5G04380 AT5G04380 AT5G38020 AT5G38020 AT5G66430
fragaria_vesca FvH4_2g09290 FvH4_2g09290 FvH4_2g09310 FvH4_3g03130
malus_domestica MD10G1071000.v1.1 MD10G1071200.v1.1 MD10G1071500.v1.1 MD10G1218400.v1.1
prunus_persica Prupe.8G092700_v2.0.a1 Prupe.8G092800_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G093000_v2.0.a1 Prupe.8G093100_v2.0.a1 Prupe.8G093200_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093600_v2.0.a1
pyrus_communis pycom10g05660 pycom10g05680 pycom10g18420
rosa_chinensis RchiOBHm_Chr1g0324421 RchiOBHm_Chr1g0324461 RchiOBHm_Chr6g0265311 RchiOBHm_Chr6g0265321 RchiOBHm_Chr6g0265361 RchiOBHm_Chr6g0265391 RchiOBHm_Chr6g0265411
rosa_laevigata RLG00000014186 RLG00000014191 RLG00000014195 RLG00000014197 RLG00000025381 RLG00000030199
rosa_multiflora Rmu_co8237181.1_g000001 Rmu_sc0001780.1_g000008 Rmu_sc0001803.1_g000020 Rmu_sc0002550.1_g000004 Rmu_sc0004082.1_g000012 Rmu_sc0008601.1_g000002 Rmu_sc0011954.1_g000001 Rmu_sc0013000.1_g000001 Rmu_sc0016482.1_g000001 Rmu_sc0018713.1_g000002 Rmu_ssc0000066.1_g000015
rosa_roxburghii Rroxscaffold_159G00432720 Rroxscaffold_159G00432790 Rroxscaffold_159G00432850 Rroxscaffold_175G00432320 Rroxscaffold_175G00432340 Rroxscaffold_175G00432350 Rroxscaffold_175G00432360 Rroxscaffold_2G00105600 Rroxscaffold_2G00105660 Rroxscaffold_4G00325530 Rroxscaffold_4G00325600 Rroxscaffold_4G00325670 Rroxscaffold_6G00424510 Rroxscaffold_7G00202680 Rroxscaffold_7G00202760 Rroxscaffold_7G00202800 Rroxscaffold_7G00202850
rosa_rugosa Rorug01G0045900 Rorug01G0046000 Rorug02G0355000 Rorug02G0355300 Rorug03G0004200 Rorug06G0016700 Rorug06G0016700 Rorug06G0016800 Rorug06G0017100
rosa_samantha Rh1AG061600 Rh1AG061700 Rh1BG052400 Rh1CG063500 Rh1CG063900 Rh1CG064100 Rh2BG415800 Rh3DG067100 Rh6AG138200 Rh6AG138400 Rh6AG138800 Rh6BG137100 Rh6BG137200 Rh6BG137600 Rh6DG121600 Rh6DG121700 Rh6DG121900
rosa_wichuraiana Rw1G005230 Rw1G005470 Rw2G033110 Rw6G011920 Rw6G011940 Rw6G011950 Rw6G011970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1061
AccIII TCCGGA 1 cut(s) 176
AciI CCGC 1 cut(s) 590
AclI AACGTT 1 cut(s) 1045
AclWI GGATC 2 cut(s) 313, 951
AcsI RAATTY 2 cut(s) 526, 829
AcuI CTGAAG 1 cut(s) 362
AfaI GTAC 1 cut(s) 533
AfiI CCNNNNNNNGG 1 cut(s) 709
AflIII ACRYGT 1 cut(s) 498
AgsI TTSAA 6 cut(s) 301, 313, 673, 754, 926, 968
AjiI CACGTC 1 cut(s) 917
AjnI CCWGG 2 cut(s) 139, 382
AjuI GAANNNNNNNTTGG 2 cut(s) 653, 685
AluBI AGCT 1 cut(s) 340
AluI AGCT 1 cut(s) 340
Alw26I GTCTC 4 cut(s) 376, 452, 590, 830
AlwI GGATC 2 cut(s) 313, 951
Aor13HI TCCGGA 1 cut(s) 176
ApoI RAATTY 2 cut(s) 526, 829
Asp700I GAANNNNTTC 2 cut(s) 296, 563
AspLEI GCGC 2 cut(s) 64, 1062
AspS9I GGNCC 2 cut(s) 649, 907
AsuHPI GGTGA 2 cut(s) 109, 799
AvaII GGWCC 2 cut(s) 649, 907
BanII GRGCYC 1 cut(s) 815
BauI CACGAG 1 cut(s) 332
BccI CCATC 3 cut(s) 23, 467, 968
BceAI ACGGC 1 cut(s) 745
BciT130I CCWGG 2 cut(s) 141, 384
BcoDI GTCTC 4 cut(s) 376, 452, 590, 830
BfaI CTAG 2 cut(s) 606, 1098
Bme1390I CCNGG 2 cut(s) 141, 384
Bme18I GGWCC 2 cut(s) 649, 907
BmgBI CACGTC 1 cut(s) 917
BmgT120I GGNCC 2 cut(s) 649, 907
BmiI GGNNCC 1 cut(s) 651
BmrFI CCNGG 2 cut(s) 141, 384
BmsI GCATC 5 cut(s) 73, 156, 158, 368, 963
BpuEI CTTGAG 1 cut(s) 500
Bsa29I ATCGAT 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 57, 382
BsaWI WCCGGW 3 cut(s) 91, 176, 256
Bsc4I CCNNNNNNNGG 1 cut(s) 709
Bse1I ACTGG 1 cut(s) 874
BseAI TCCGGA 1 cut(s) 176
BseBI CCWGG 2 cut(s) 141, 384
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 57, 382
BseGI GGATG 2 cut(s) 173, 383
BseLI CCNNNNNNNGG 1 cut(s) 709
BseMII CTCAG 2 cut(s) 387, 708
BseNI ACTGG 1 cut(s) 874
BseRI GAGGAG 1 cut(s) 692
BshVI ATCGAT 1 cut(s) 264
BsiSI CCGG 3 cut(s) 92, 177, 257
BslI CCNNNNNNNGG 1 cut(s) 709
BsmAI GTCTC 4 cut(s) 376, 452, 590, 830
BsmI GAATGC 1 cut(s) 588
Bsp1286I GDGCHC 1 cut(s) 815
Bsp13I TCCGGA 1 cut(s) 176
Bsp143I GATC 6 cut(s) 261, 280, 318, 822, 956, 1006
BspACI CCGC 1 cut(s) 590
BspCNI CTCAG 2 cut(s) 386, 709
BspDI ATCGAT 1 cut(s) 264
BspEI TCCGGA 1 cut(s) 176
BspLI GGNNCC 1 cut(s) 651
BspPI GGATC 2 cut(s) 313, 951
BspQI GCTCTTC 1 cut(s) 178
BsrI ACTGG 1 cut(s) 874
BssECI CCNNGG 2 cut(s) 57, 382
BssMI GATC 6 cut(s) 261, 280, 318, 822, 956, 1006
BssSI CACGAG 1 cut(s) 332
Bst2BI CACGAG 1 cut(s) 332
Bst2UI CCWGG 2 cut(s) 141, 384
Bst4CI ACNGT 3 cut(s) 41, 770, 1075
Bst6I CTCTTC 3 cut(s) 178, 426, 588
BstC8I GCNNGC 1 cut(s) 588
BstDEI CTNAG 4 cut(s) 373, 506, 578, 717
BstF5I GGATG 2 cut(s) 173, 383
BstHHI GCGC 2 cut(s) 64, 1062
BstKTI GATC 6 cut(s) 264, 283, 321, 825, 959, 1009
BstMAI GTCTC 4 cut(s) 376, 452, 590, 830
BstMBI GATC 6 cut(s) 261, 280, 318, 822, 956, 1006
BstNI CCWGG 2 cut(s) 141, 384
BstNSI RCATGY 1 cut(s) 1041
BstSCI CCNGG 2 cut(s) 139, 382
BstX2I RGATCY 1 cut(s) 956
BstXI CCANNNNNNTGG 1 cut(s) 242
BstYI RGATCY 1 cut(s) 956
Bsu15I ATCGAT 1 cut(s) 264
BsuTUI ATCGAT 1 cut(s) 264
BtrI CACGTC 1 cut(s) 917
BtsCI GGATG 2 cut(s) 173, 383
Cac8I GCNNGC 1 cut(s) 588
CfoI GCGC 2 cut(s) 64, 1062
Cfr13I GGNCC 2 cut(s) 649, 907
ClaI ATCGAT 1 cut(s) 264
CseI GACGC 1 cut(s) 621
Csp6I GTAC 1 cut(s) 532
CviAII CATG 5 cut(s) 16, 516, 616, 1034, 1038
CviQI GTAC 1 cut(s) 532
DdeI CTNAG 4 cut(s) 373, 506, 578, 717
DpnI GATC 6 cut(s) 263, 282, 320, 824, 958, 1008
DpnII GATC 6 cut(s) 261, 280, 318, 822, 956, 1006
Eam1104I CTCTTC 3 cut(s) 178, 426, 588
EarI CTCTTC 3 cut(s) 178, 426, 588
Eco24I GRGCYC 1 cut(s) 815
Eco47I GGWCC 2 cut(s) 649, 907
Eco57I CTGAAG 1 cut(s) 362
EcoRII CCWGG 2 cut(s) 139, 382
EcoT22I ATGCAT 1 cut(s) 1039
EcoT38I GRGCYC 1 cut(s) 815
FaeI CATG 5 cut(s) 19, 519, 619, 1037, 1041
FalI AAGNNNNNCTT 2 cut(s) 61, 93
FatI CATG 5 cut(s) 15, 515, 615, 1033, 1037
FokI GGATG 2 cut(s) 180, 390
FriOI GRGCYC 1 cut(s) 815
FspBI CTAG 2 cut(s) 606, 1098
FspI TGCGCA 1 cut(s) 1061
GlaI GCGC 2 cut(s) 63, 1061
HapII CCGG 3 cut(s) 92, 177, 257
HgaI GACGC 1 cut(s) 621
HhaI GCGC 2 cut(s) 64, 1062
Hin1II CATG 5 cut(s) 19, 519, 619, 1037, 1041
Hin6I GCGC 2 cut(s) 62, 1060
HinP1I GCGC 2 cut(s) 62, 1060
HincII GTYRAC 3 cut(s) 214, 1043, 1095
HindII GTYRAC 3 cut(s) 214, 1043, 1095
HinfI GANTC 4 cut(s) 547, 695, 734, 746
HpaI GTTAAC 1 cut(s) 214
HpaII CCGG 3 cut(s) 92, 177, 257
HphI GGTGA 2 cut(s) 109, 799
Hpy166II GTNNAC 6 cut(s) 112, 214, 611, 907, 1043, 1095
Hpy188I TCNGA 6 cut(s) 58, 82, 205, 694, 718, 739
Hpy188III TCNNGA 7 cut(s) 177, 287, 375, 470, 773, 802, 820
Hpy8I GTNNAC 6 cut(s) 112, 214, 611, 907, 1043, 1095
Hpy99I CGWCG 1 cut(s) 921
HpyAV CCTTC 5 cut(s) 13, 333, 337, 724, 784
HpyCH4III ACNGT 3 cut(s) 41, 770, 1075
HpyCH4IV ACGT 3 cut(s) 498, 916, 1045
HpyCH4V TGCA 4 cut(s) 47, 86, 147, 1037
HpyF3I CTNAG 4 cut(s) 373, 506, 578, 717
HpySE526I ACGT 3 cut(s) 498, 916, 1045
Hsp92II CATG 5 cut(s) 19, 519, 619, 1037, 1041
HspAI GCGC 2 cut(s) 62, 1060
Kpn2I TCCGGA 1 cut(s) 176
KspAI GTTAAC 1 cut(s) 214
Kzo9I GATC 6 cut(s) 261, 280, 318, 822, 956, 1006
LguI GCTCTTC 1 cut(s) 178
LweI GCATC 5 cut(s) 73, 156, 158, 368, 963
MaeI CTAG 2 cut(s) 606, 1098
MaeII ACGT 3 cut(s) 498, 916, 1045
MaeIII GTNAC 1 cut(s) 1046
MalI GATC 6 cut(s) 263, 282, 320, 824, 958, 1008
MboI GATC 6 cut(s) 261, 280, 318, 822, 956, 1006
MboII GAAGA 6 cut(s) 165, 275, 355, 413, 505, 605
MflI RGATCY 1 cut(s) 956
MhlI GDGCHC 1 cut(s) 815
MluCI AATT 5 cut(s) 52, 361, 526, 829, 1078
Mph1103I ATGCAT 1 cut(s) 1039
MroI TCCGGA 1 cut(s) 176
MroXI GAANNNNTTC 2 cut(s) 296, 563
MseI TTAA 5 cut(s) 213, 354, 555, 933, 1077
MslI CAYNNNNRTG 2 cut(s) 42, 152
MspI CCGG 3 cut(s) 92, 177, 257
MspR9I CCNGG 2 cut(s) 141, 384
Mva1269I GAATGC 1 cut(s) 588
MvaI CCWGG 2 cut(s) 141, 384
NdeII GATC 6 cut(s) 261, 280, 318, 822, 956, 1006
NlaIII CATG 5 cut(s) 19, 519, 619, 1037, 1041
NlaIV GGNNCC 1 cut(s) 651
NsbI TGCGCA 1 cut(s) 1061
NsiI ATGCAT 1 cut(s) 1039
NspI RCATGY 1 cut(s) 1041
PciSI GCTCTTC 1 cut(s) 178
PctI GAATGC 1 cut(s) 588
PdmI GAANNNNTTC 2 cut(s) 296, 563
PfeI GAWTC 4 cut(s) 547, 695, 734, 746
Psp1406I AACGTT 1 cut(s) 1045
Psp6I CCWGG 2 cut(s) 139, 382
PspGI CCWGG 2 cut(s) 139, 382
PspN4I GGNNCC 1 cut(s) 651
PspPI GGNCC 2 cut(s) 649, 907
PsuI RGATCY 1 cut(s) 956
RsaI GTAC 1 cut(s) 533
RsaNI GTAC 1 cut(s) 532
RseI CAYNNNNRTG 2 cut(s) 42, 152
SapI GCTCTTC 1 cut(s) 178
SaqAI TTAA 5 cut(s) 213, 354, 555, 933, 1077
Sau3AI GATC 6 cut(s) 261, 280, 318, 822, 956, 1006
Sau96I GGNCC 2 cut(s) 649, 907
ScrFI CCNGG 2 cut(s) 141, 384
SduI GDGCHC 1 cut(s) 815
SetI ASST 7 cut(s) 99, 342, 501, 789, 919, 1048, 1058
SfaNI GCATC 5 cut(s) 73, 156, 158, 368, 963
SinI GGWCC 2 cut(s) 649, 907
SmiMI CAYNNNNRTG 2 cut(s) 42, 152
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 5 cut(s) 52, 361, 526, 829, 1078
SsiI CCGC 1 cut(s) 590
SspI AATATT 1 cut(s) 843
SspMI CTAG 2 cut(s) 606, 1098
StyD4I CCNGG 2 cut(s) 139, 382
TaaI ACNGT 3 cut(s) 41, 770, 1075
TaiI ACGT 3 cut(s) 501, 919, 1048
TaqI TCGA 5 cut(s) 264, 705, 801, 819, 919
TasI AATT 5 cut(s) 52, 361, 526, 829, 1078
TfiI GAWTC 4 cut(s) 547, 695, 734, 746
Tru1I TTAA 5 cut(s) 213, 354, 555, 933, 1077
Tru9I TTAA 5 cut(s) 213, 354, 555, 933, 1077
TspDTI ATGAA 7 cut(s) 32, 116, 362, 413, 426, 792, 849
VpaK11BI GGWCC 2 cut(s) 649, 907
XapI RAATTY 2 cut(s) 526, 829
XceI RCATGY 1 cut(s) 1041
XmnI GAANNNNTTC 2 cut(s) 296, 563
XspI CTAG 2 cut(s) 606, 1098
Zsp2I ATGCAT 1 cut(s) 1039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.