Rw6G011970

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000342
Physical Location & Seq
Forward (+)
21695299 .. 21698574
3276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G011970.1

Sequence Viewer

Length: 1128 bp
ATGTTTAATTACACCAACCAAAACAAAATGGAGGTAGAGCAGGTTCTTCACATGAATGGCGGAGATGGCAAAACAAGCTACGCAAACCACTCACTTCTTCAAAGAGCTGTGATTTCGACGGTGAAGCCCATAGTGGACGCAAGCATAGAGGAGCTCTGCAGCACTCTCTTCCCAGAGTGTCTGAAAATAGCAGACTTGGGATGCTCTTCGGGACCCAATACCCTTCTGGTGGTATCAGACATCATGGACAACATCCAGAACACGTTTCGGAAGCTGAACCTTCCCCCACCATCGCTTCAAGCATTCTTGAATGACCTTCCCAGGAACGATTTCAACACGGTGTTCAGGTCACTGCCTGGCTTCTATAAGAAGCTCGATGAAGAACCTGATAAAAAGTCGGGTCCTTGTTTCATTGCAGCAATGCCTGGTTCCTTTTATGGGAGGCTCTTTCCTAACAACTCTCTCCACTTTGTTCATTCTTCTTATGCTCTCATGTGGATCTCTGAGGTTCCAAAAGGTTTGGTAACAAAAGCAGGAGAGGGACTGAACAAGGGGAACATATACATAGCCAAGACGAGCTCACCTGCTGTGTTTAATGAATACTTTGAGCAATTCAAAAGGGACTTCACACTCTTTCTGAGATCTCGGGCACAAGAACTAGTCCCAGGAGGTAGTATGGTCCTCACAACCATGGGCAGCATAACGAGCAATGATCCGCTCTGCATTTGGGAATTTGTCGGATTGAAACTCAATGACATGGTTTTAGATGGTTTGATTGAGAAGGAAAAGTTGGACATATTCAATTTGCCATACTATGCACCCACAAGATATGAGGTACAGGAGGTGATCGAGGCTGAAGGGTCTTTTACTTTGCAAAACCTTGAAGCTTTCAGAAATGACTGGGACTCTTACATAAAACAAGCTAAAAGTGACCTTGACAAGAAAGCAAGGGCTGCAATACTCTCCACTGACATAAGGGCTGTGGGAGAGCCTATTCTGGCCAGCCATTTCGGAGAGGAAGCAATGGACGAATTGTTCAGTAGGTTCGAAGAAAATGTTCTCGATCACATGGAAAGAGAGAAGTGCCAGTTCATTAACCTGGTTATCTCATTGACTAAGAAGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

375

Amino Acids

42.34

Weight (kDa)

5.26

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 49 - 373 9.5e-123 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14060 AT2G14060 AT3G11480 AT3G21950 AT3G21950 AT5G04370 AT5G04370 AT5G04370 AT5G04380 AT5G04380 AT5G04380 AT5G38020 AT5G38020 AT5G66430
fragaria_vesca FvH4_2g09290 FvH4_2g09290 FvH4_2g09310 FvH4_3g03130
malus_domestica MD10G1071000.v1.1 MD10G1071200.v1.1 MD10G1071500.v1.1 MD10G1218400.v1.1
prunus_persica Prupe.8G092700_v2.0.a1 Prupe.8G092800_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G093000_v2.0.a1 Prupe.8G093100_v2.0.a1 Prupe.8G093200_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093600_v2.0.a1
pyrus_communis pycom10g05660 pycom10g05680 pycom10g18420
rosa_chinensis RchiOBHm_Chr1g0324421 RchiOBHm_Chr1g0324461 RchiOBHm_Chr6g0265311 RchiOBHm_Chr6g0265321 RchiOBHm_Chr6g0265361 RchiOBHm_Chr6g0265391 RchiOBHm_Chr6g0265411
rosa_laevigata RLG00000014186 RLG00000014191 RLG00000014195 RLG00000014197 RLG00000025381 RLG00000030199
rosa_multiflora Rmu_co8237181.1_g000001 Rmu_sc0001780.1_g000008 Rmu_sc0001803.1_g000020 Rmu_sc0002550.1_g000004 Rmu_sc0004082.1_g000012 Rmu_sc0008601.1_g000002 Rmu_sc0011954.1_g000001 Rmu_sc0013000.1_g000001 Rmu_sc0016482.1_g000001 Rmu_sc0018713.1_g000002 Rmu_ssc0000066.1_g000015
rosa_roxburghii Rroxscaffold_159G00432720 Rroxscaffold_159G00432790 Rroxscaffold_159G00432850 Rroxscaffold_175G00432320 Rroxscaffold_175G00432340 Rroxscaffold_175G00432350 Rroxscaffold_175G00432360 Rroxscaffold_2G00105600 Rroxscaffold_2G00105660 Rroxscaffold_4G00325530 Rroxscaffold_4G00325600 Rroxscaffold_4G00325670 Rroxscaffold_6G00424510 Rroxscaffold_7G00202680 Rroxscaffold_7G00202760 Rroxscaffold_7G00202800 Rroxscaffold_7G00202850
rosa_rugosa Rorug01G0045900 Rorug01G0046000 Rorug02G0355000 Rorug02G0355300 Rorug03G0004200 Rorug06G0016700 Rorug06G0016700 Rorug06G0016800 Rorug06G0017100
rosa_samantha Rh1AG061600 Rh1AG061700 Rh1BG052400 Rh1CG063500 Rh1CG063900 Rh1CG064100 Rh2BG415800 Rh3DG067100 Rh6AG138200 Rh6AG138400 Rh6AG138800 Rh6BG137100 Rh6BG137200 Rh6BG137600 Rh6DG121600 Rh6DG121700 Rh6DG121900
rosa_wichuraiana Rw1G005230 Rw1G005470 Rw2G033110 Rw6G011920 Rw6G011940 Rw6G011950 Rw6G011970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 592
Acc36I ACCTGC 2 cut(s) 31, 592
AccBSI CCGCTC 1 cut(s) 718
AciI CCGC 2 cut(s) 60, 716
AclWI GGATC 2 cut(s) 506, 707
AcoI YGGCCR 1 cut(s) 999
AcsI RAATTY 1 cut(s) 731
AcuI CTGAAG 1 cut(s) 876
AfaI GTAC 1 cut(s) 837
AfiI CCNNNNNNNGG 2 cut(s) 229, 438
AflIII ACRYGT 1 cut(s) 261
AgsI TTSAA 8 cut(s) 101, 299, 310, 334, 616, 745, 802, 884
AhlI ACTAGT 1 cut(s) 658
AjnI CCWGG 5 cut(s) 320, 355, 424, 664, 1098
AjuI GAANNNNNNNTTGG 2 cut(s) 773, 805
AloI GAACNNNNNNTCC 2 cut(s) 1019, 1051
AluBI AGCT 8 cut(s) 78, 107, 154, 274, 373, 579, 887, 923
AluI AGCT 8 cut(s) 78, 107, 154, 274, 373, 579, 887, 923
Alw21I GWGCWC 2 cut(s) 156, 581
AlwI GGATC 2 cut(s) 506, 707
Ama87I CYCGRG 1 cut(s) 645
AoxI GGCC 1 cut(s) 999
ApeKI GCWGC 4 cut(s) 159, 416, 696, 953
ApoI RAATTY 1 cut(s) 731
Asp700I GAANNNNTTC 2 cut(s) 329, 1056
AspS9I GGNCC 3 cut(s) 212, 401, 679
AsuHPI GGTGA 3 cut(s) 133, 573, 856
AsuII TTCGAA 1 cut(s) 1047
AvaI CYCGRG 1 cut(s) 645
AvaII GGWCC 3 cut(s) 212, 401, 679
BaeGI GKGCMC 1 cut(s) 652
BalI TGGCCA 1 cut(s) 1001
BanII GRGCYC 2 cut(s) 156, 581
Bbv12I GWGCWC 2 cut(s) 156, 581
BbvI GCAGC 4 cut(s) 171, 428, 708, 940
BccI CCATC 3 cut(s) 59, 298, 761
BciT130I CCWGG 5 cut(s) 322, 357, 426, 666, 1100
BcuI ACTAGT 1 cut(s) 658
BfaI CTAG 1 cut(s) 659
BfmI CTRYAG 1 cut(s) 157
BfuAI ACCTGC 2 cut(s) 31, 592
BglII AGATCT 1 cut(s) 641
BisI GCNGC 4 cut(s) 160, 417, 697, 954
BlsI GCNGC 4 cut(s) 161, 418, 698, 955
Bme1390I CCNGG 5 cut(s) 322, 357, 426, 666, 1100
Bme18I GGWCC 3 cut(s) 212, 401, 679
BmeT110I CYCGRG 1 cut(s) 645
BmgT120I GGNCC 3 cut(s) 212, 401, 679
BmiI GGNNCC 5 cut(s) 213, 214, 402, 430, 510
BmrFI CCNGG 5 cut(s) 322, 357, 426, 666, 1100
BmrI ACTGGG 1 cut(s) 910
BmsI GCATC 1 cut(s) 191
BmuI ACTGGG 1 cut(s) 910
Bpu14I TTCGAA 1 cut(s) 1047
BsaJI CCNNGG 3 cut(s) 320, 664, 690
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 438
Bse1I ACTGG 2 cut(s) 905, 1087
Bse3DI GCAATG 4 cut(s) 411, 426, 715, 1029
BseBI CCWGG 5 cut(s) 322, 357, 426, 666, 1100
BseDI CCNNGG 3 cut(s) 320, 664, 690
BseGI GGATG 2 cut(s) 206, 252
BseLI CCNNNNNNNGG 2 cut(s) 229, 438
BseMI GCAATG 4 cut(s) 411, 426, 715, 1029
BseMII CTCAG 2 cut(s) 495, 629
BseNI ACTGG 2 cut(s) 905, 1087
BseRI GAGGAG 1 cut(s) 164
BseSI GKGCMC 1 cut(s) 652
BseXI GCAGC 4 cut(s) 171, 428, 708, 940
BshFI GGCC 1 cut(s) 1001
BsiHKAI GWGCWC 2 cut(s) 156, 581
BsiHKCI CYCGRG 1 cut(s) 645
BslFI GGGAC 5 cut(s) 225, 555, 635, 647, 917
BslI CCNNNNNNNGG 2 cut(s) 229, 438
BsmFI GGGAC 5 cut(s) 225, 555, 635, 647, 917
BsmI GAATGC 1 cut(s) 302
BsnI GGCC 1 cut(s) 1001
BsoBI CYCGRG 1 cut(s) 645
Bsp119I TTCGAA 1 cut(s) 1047
Bsp1286I GDGCHC 3 cut(s) 156, 581, 652
Bsp143I GATC 5 cut(s) 498, 641, 712, 846, 1063
Bsp19I CCATGG 1 cut(s) 690
BspACI CCGC 2 cut(s) 60, 716
BspANI GGCC 1 cut(s) 1001
BspCNI CTCAG 2 cut(s) 496, 630
BspLI GGNNCC 5 cut(s) 213, 214, 402, 430, 510
BspMAI CTGCAG 1 cut(s) 161
BspMI ACCTGC 2 cut(s) 31, 592
BspPI GGATC 2 cut(s) 506, 707
BspQI GCTCTTC 1 cut(s) 211
BspT104I TTCGAA 1 cut(s) 1047
BsrBI CCGCTC 1 cut(s) 718
BsrDI GCAATG 4 cut(s) 411, 426, 715, 1029
BsrI ACTGG 2 cut(s) 905, 1087
BssECI CCNNGG 3 cut(s) 320, 664, 690
BssMI GATC 5 cut(s) 498, 641, 712, 846, 1063
BssT1I CCWWGG 1 cut(s) 690
Bst2UI CCWGG 5 cut(s) 322, 357, 426, 666, 1100
Bst4CI ACNGT 2 cut(s) 121, 340
Bst6I CTCTTC 2 cut(s) 173, 211
BstAPI GCANNNNNTGC 1 cut(s) 953
BstBI TTCGAA 1 cut(s) 1047
BstC8I GCNNGC 2 cut(s) 142, 1003
BstDEI CTNAG 3 cut(s) 504, 638, 1116
BstDSI CCRYGG 1 cut(s) 690
BstF5I GGATG 2 cut(s) 206, 252
BstKTI GATC 5 cut(s) 501, 644, 715, 849, 1066
BstMBI GATC 5 cut(s) 498, 641, 712, 846, 1063
BstMWI GCNNNNNNNGC 4 cut(s) 66, 75, 705, 953
BstNI CCWGG 5 cut(s) 322, 357, 426, 666, 1100
BstSCI CCNGG 5 cut(s) 320, 355, 424, 664, 1098
BstSFI CTRYAG 1 cut(s) 157
BstSLI GKGCMC 1 cut(s) 652
BstV1I GCAGC 4 cut(s) 171, 428, 708, 940
BstX2I RGATCY 2 cut(s) 498, 641
BstYI RGATCY 2 cut(s) 498, 641
BsuRI GGCC 1 cut(s) 1001
BtgI CCRYGG 1 cut(s) 690
BtgZI GCGATG 1 cut(s) 276
BtsCI GGATG 2 cut(s) 206, 252
BtsI GCAGTG 1 cut(s) 350
BtsIMutI CAGTG 2 cut(s) 350, 966
BveI ACCTGC 2 cut(s) 31, 592
Cac8I GCNNGC 2 cut(s) 142, 1003
Cfr13I GGNCC 3 cut(s) 212, 401, 679
CseI GACGC 1 cut(s) 146
CsiI ACCWGGT 1 cut(s) 1098
Csp6I GTAC 1 cut(s) 836
CviAII CATG 6 cut(s) 52, 244, 493, 691, 757, 1069
CviQI GTAC 1 cut(s) 836
DdeI CTNAG 3 cut(s) 504, 638, 1116
DpnI GATC 5 cut(s) 500, 643, 714, 848, 1065
DpnII GATC 5 cut(s) 498, 641, 712, 846, 1063
EaeI YGGCCR 1 cut(s) 999
Eam1104I CTCTTC 2 cut(s) 173, 211
EarI CTCTTC 2 cut(s) 173, 211
EciI GGCGGA 1 cut(s) 75
Ecl136II GAGCTC 2 cut(s) 154, 579
Eco130I CCWWGG 1 cut(s) 690
Eco24I GRGCYC 2 cut(s) 156, 581
Eco47I GGWCC 3 cut(s) 212, 401, 679
Eco53kI GAGCTC 2 cut(s) 154, 579
Eco57I CTGAAG 1 cut(s) 876
Eco88I CYCGRG 1 cut(s) 645
EcoICRI GAGCTC 2 cut(s) 154, 579
EcoO109I RGGNCCY 2 cut(s) 212, 401
EcoRII CCWGG 5 cut(s) 320, 355, 424, 664, 1098
EcoT14I CCWWGG 1 cut(s) 690
EcoT38I GRGCYC 2 cut(s) 156, 581
ErhI CCWWGG 1 cut(s) 690
FaeI CATG 6 cut(s) 55, 247, 496, 694, 760, 1072
FaqI GGGAC 5 cut(s) 225, 555, 635, 647, 917
FatI CATG 6 cut(s) 51, 243, 492, 690, 756, 1068
Fnu4HI GCNGC 4 cut(s) 160, 417, 697, 954
FokI GGATG 2 cut(s) 213, 239
FriOI GRGCYC 2 cut(s) 156, 581
Fsp4HI GCNGC 4 cut(s) 160, 417, 697, 954
FspBI CTAG 1 cut(s) 659
GluI GCNGC 4 cut(s) 160, 417, 697, 954
HaeIII GGCC 1 cut(s) 1001
HgaI GACGC 1 cut(s) 146
Hin1II CATG 6 cut(s) 55, 247, 496, 694, 760, 1072
HindIII AAGCTT 1 cut(s) 885
HinfI GANTC 1 cut(s) 905
HphI GGTGA 3 cut(s) 133, 573, 856
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 8 cut(s) 183, 238, 270, 505, 639, 740, 893, 1013
Hpy188III TCNNGA 4 cut(s) 210, 256, 307, 1061
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 5 cut(s) 233, 290, 326, 775, 851
HpyCH4III ACNGT 2 cut(s) 121, 340
HpyCH4IV ACGT 1 cut(s) 263
HpyCH4V TGCA 6 cut(s) 159, 416, 723, 818, 874, 956
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 75, 705, 953
HpyF3I CTNAG 3 cut(s) 504, 638, 1116
HpySE526I ACGT 1 cut(s) 263
Hsp92II CATG 6 cut(s) 55, 247, 496, 694, 760, 1072
KflI GGGWCCC 1 cut(s) 212
Kzo9I GATC 5 cut(s) 498, 641, 712, 846, 1063
LguI GCTCTTC 1 cut(s) 211
LmnI GCTCC 1 cut(s) 151
Lsp1109I GCAGC 4 cut(s) 171, 428, 708, 940
LweI GCATC 1 cut(s) 191
MabI ACCWGGT 1 cut(s) 1098
MaeI CTAG 1 cut(s) 659
MaeII ACGT 1 cut(s) 263
MaeIII GTNAC 3 cut(s) 348, 523, 929
MalI GATC 5 cut(s) 500, 643, 714, 848, 1065
MbiI CCGCTC 1 cut(s) 718
MboI GATC 5 cut(s) 498, 641, 712, 846, 1063
MboII GAAGA 7 cut(s) 38, 89, 160, 198, 392, 471, 1061
MflI RGATCY 2 cut(s) 498, 641
MhlI GDGCHC 3 cut(s) 156, 581, 652
MlsI TGGCCA 1 cut(s) 1001
MluCI AATT 5 cut(s) 7, 611, 731, 802, 1031
MluNI TGGCCA 1 cut(s) 1001
MlyI GAGTC 1 cut(s) 899
MmeI TCCRAC 2 cut(s) 718, 771
Mox20I TGGCCA 1 cut(s) 1001
MroXI GAANNNNTTC 2 cut(s) 329, 1056
MscI TGGCCA 1 cut(s) 1001
MseI TTAA 3 cut(s) 6, 594, 1095
MslI CAYNNNNRTG 2 cut(s) 54, 689
Msp20I TGGCCA 1 cut(s) 1001
MspR9I CCNGG 5 cut(s) 322, 357, 426, 666, 1100
Mva1269I GAATGC 1 cut(s) 302
MvaI CCWGG 5 cut(s) 322, 357, 426, 666, 1100
MwoI GCNNNNNNNGC 4 cut(s) 66, 75, 705, 953
NcoI CCATGG 1 cut(s) 690
NdeII GATC 5 cut(s) 498, 641, 712, 846, 1063
NlaIII CATG 6 cut(s) 55, 247, 496, 694, 760, 1072
NlaIV GGNNCC 5 cut(s) 213, 214, 402, 430, 510
NmuCI GTSAC 2 cut(s) 348, 929
NspV TTCGAA 1 cut(s) 1047
PaqCI CACCTGC 1 cut(s) 592
PciSI GCTCTTC 1 cut(s) 211
PctI GAATGC 1 cut(s) 302
PdmI GAANNNNTTC 2 cut(s) 329, 1056
PkrI GCNGC 4 cut(s) 161, 418, 698, 955
PleI GAGTC 1 cut(s) 899
PpsI GAGTC 1 cut(s) 899
PpuMI RGGWCCY 2 cut(s) 212, 401
Psp124BI GAGCTC 2 cut(s) 156, 581
Psp5II RGGWCCY 2 cut(s) 212, 401
Psp6I CCWGG 5 cut(s) 320, 355, 424, 664, 1098
PspGI CCWGG 5 cut(s) 320, 355, 424, 664, 1098
PspN4I GGNNCC 5 cut(s) 213, 214, 402, 430, 510
PspPI GGNCC 3 cut(s) 212, 401, 679
PspPPI RGGWCCY 2 cut(s) 212, 401
PsrI GAACNNNNNNTAC 2 cut(s) 27, 59
PstI CTGCAG 1 cut(s) 161
PsuI RGATCY 2 cut(s) 498, 641
RsaI GTAC 1 cut(s) 837
RsaNI GTAC 1 cut(s) 836
RseI CAYNNNNRTG 2 cut(s) 54, 689
SacI GAGCTC 2 cut(s) 156, 581
SapI GCTCTTC 1 cut(s) 211
SaqAI TTAA 3 cut(s) 6, 594, 1095
SatI GCNGC 4 cut(s) 160, 417, 697, 954
Sau3AI GATC 5 cut(s) 498, 641, 712, 846, 1063
Sau96I GGNCC 3 cut(s) 212, 401, 679
SchI GAGTC 1 cut(s) 899
ScrFI CCNGG 5 cut(s) 322, 357, 426, 666, 1100
SduI GDGCHC 3 cut(s) 156, 581, 652
SexAI ACCWGGT 1 cut(s) 1098
SfaNI GCATC 1 cut(s) 191
SfcI CTRYAG 1 cut(s) 157
SfuI TTCGAA 1 cut(s) 1047
SinI GGWCC 3 cut(s) 212, 401, 679
SmiMI CAYNNNNRTG 2 cut(s) 54, 689
SpeI ACTAGT 1 cut(s) 658
Sse9I AATT 5 cut(s) 7, 611, 731, 802, 1031
SsiI CCGC 2 cut(s) 60, 716
SspMI CTAG 1 cut(s) 659
SstI GAGCTC 2 cut(s) 156, 581
StyD4I CCNGG 5 cut(s) 320, 355, 424, 664, 1098
StyI CCWWGG 1 cut(s) 690
TaaI ACNGT 2 cut(s) 121, 340
TaiI ACGT 1 cut(s) 266
TaqI TCGA 5 cut(s) 116, 375, 849, 1047, 1062
TasI AATT 5 cut(s) 7, 611, 731, 802, 1031
Tru1I TTAA 3 cut(s) 6, 594, 1095
Tru9I TTAA 3 cut(s) 6, 594, 1095
TscAI CASTG 2 cut(s) 357, 973
TseFI GTSAC 2 cut(s) 348, 929
TseI GCWGC 4 cut(s) 159, 416, 696, 953
Tsp45I GTSAC 2 cut(s) 348, 929
TspDTI ATGAA 6 cut(s) 68, 393, 400, 464, 612, 1081
TspRI CASTG 2 cut(s) 357, 973
VpaK11BI GGWCC 3 cut(s) 212, 401, 679
XapI RAATTY 1 cut(s) 731
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
XmnI GAANNNNTTC 2 cut(s) 329, 1056
XspI CTAG 1 cut(s) 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.