Rroxscaffold_7G00202680

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000342
Physical Location & Seq
Reverse (-)
50885605 .. 50887547
1943 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00202680.1

Sequence Viewer

Length: 1173 bp
ATGGAGGTAGAGCAGGTTCTTCACATGAATGGCGGAGATGGCAAAACAAGCTACGCAAACCACTCACTTCTTCAAAGAGCTGTGATTTCGACGGTGAAGCCCATAGTGGACGCAAGCATAGAGGAGCTCTGCAGCACTCTCTTCCCAGAGTGTCTGAAAATAGCAGACTTGGGATGCTCTTCGGGACCCAATACCCTTCTGGTGGTATCAGACATCATGGACAACATCCGGAACACGTTTCGGAAGCTGAACCTTCCTCCACCATCGCTTCAAGCATTCTTGAATGACCTTCCCAGGAACGATTTCAACACGGTGTTCAAGTCACTGCCTGGCTTCTATAAGAAGCTAGATGAAGAACCTGATAAAAAGTCGGGTCCTTGTTTCATTGCAGCAATGCCTGGTTCCTTTTATGGGAGGCTCTTTCCTAACAACTCTCTCCACTTTGTTCATTCTTCTTATGCTCTCATGTGGATCTCTGAGGCTGTAAACAAGCCTCAGATTCATGGCCTAGAGCCATGTCAATTTCTCCTCGCCACATCAAATTTTCACAAAGTTCCAAAAGGTTTGGTAACAAAAGCAGGAGAGGGACTGAACAAGGGGAACATATACATAGCCAAGACGAGCTCACCTGCTGTGTTTAATGAATACTTTGAGCAATTCAAAAGGGACTTCACACTCTTTCTGAGATCTCGGGCACAAGAACTAGTCCCAGGAGGTAGTATGGTCCTCACAACCATGGGCAGCATAACGAGCAATGATCCCCTCTGCATTTGGGAATTCGTCGGATTGAAACTCAATGACATGGTTTTAGATGGTTTGATTGAGAAGGAAAAGTTGGACACATTCAATTTGCCATACTATGCACCCACAAGATATGAGGTAAAGGAGGTGATCGAGGCTGAAGGGTCTTTTACTTTACAAAACCTTGAAGCTTTCAGAAATGACTGGGACTCTTACATAAAACAAGCTAAAAGTGACCTTGACAAGAAAGCAAGGGCTGCAATACTCTCCACTGACATAAGGGCTGTGGGAGAGCCTATTCTGGCCAGCCATTTCGGAGAGGAAGCAATGGACGAATTGTTCAGTAGGTTCGAAGAAAATGTTCTCGATCACATGGAAAGAGAGAATTGCCAGTTCATTAACCTGGTTATCTCATTGACTAAGAAGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

390

Amino Acids

43.84

Weight (kDa)

5.51

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 40 - 162 9.3e-53 SAM dependent carboxyl methyltransferase
Methyltransf_7 PF03492 184 - 388 1e-62 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14060 AT2G14060 AT3G11480 AT3G21950 AT3G21950 AT5G04370 AT5G04370 AT5G04370 AT5G04380 AT5G04380 AT5G04380 AT5G38020 AT5G38020 AT5G66430
fragaria_vesca FvH4_2g09290 FvH4_2g09290 FvH4_2g09310 FvH4_3g03130
malus_domestica MD10G1071000.v1.1 MD10G1071200.v1.1 MD10G1071500.v1.1 MD10G1218400.v1.1
prunus_persica Prupe.8G092700_v2.0.a1 Prupe.8G092800_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G093000_v2.0.a1 Prupe.8G093100_v2.0.a1 Prupe.8G093200_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093600_v2.0.a1
pyrus_communis pycom10g05660 pycom10g05680 pycom10g18420
rosa_chinensis RchiOBHm_Chr1g0324421 RchiOBHm_Chr1g0324461 RchiOBHm_Chr6g0265311 RchiOBHm_Chr6g0265321 RchiOBHm_Chr6g0265361 RchiOBHm_Chr6g0265391 RchiOBHm_Chr6g0265411
rosa_laevigata RLG00000014186 RLG00000014191 RLG00000014195 RLG00000014197 RLG00000025381 RLG00000030199
rosa_multiflora Rmu_co8237181.1_g000001 Rmu_sc0001780.1_g000008 Rmu_sc0001803.1_g000020 Rmu_sc0002550.1_g000004 Rmu_sc0004082.1_g000012 Rmu_sc0008601.1_g000002 Rmu_sc0011954.1_g000001 Rmu_sc0013000.1_g000001 Rmu_sc0016482.1_g000001 Rmu_sc0018713.1_g000002 Rmu_ssc0000066.1_g000015
rosa_roxburghii Rroxscaffold_159G00432720 Rroxscaffold_159G00432790 Rroxscaffold_159G00432850 Rroxscaffold_175G00432320 Rroxscaffold_175G00432340 Rroxscaffold_175G00432350 Rroxscaffold_175G00432360 Rroxscaffold_2G00105600 Rroxscaffold_2G00105660 Rroxscaffold_4G00325530 Rroxscaffold_4G00325600 Rroxscaffold_4G00325670 Rroxscaffold_6G00424510 Rroxscaffold_7G00202680 Rroxscaffold_7G00202760 Rroxscaffold_7G00202800 Rroxscaffold_7G00202850
rosa_rugosa Rorug01G0045900 Rorug01G0046000 Rorug02G0355000 Rorug02G0355300 Rorug03G0004200 Rorug06G0016700 Rorug06G0016700 Rorug06G0016800 Rorug06G0017100
rosa_samantha Rh1AG061600 Rh1AG061700 Rh1BG052400 Rh1CG063500 Rh1CG063900 Rh1CG064100 Rh2BG415800 Rh3DG067100 Rh6AG138200 Rh6AG138400 Rh6AG138800 Rh6BG137100 Rh6BG137200 Rh6BG137600 Rh6DG121600 Rh6DG121700 Rh6DG121900
rosa_wichuraiana Rw1G005230 Rw1G005470 Rw2G033110 Rw6G011920 Rw6G011940 Rw6G011950 Rw6G011970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 637
Acc36I ACCTGC 2 cut(s) 4, 637
AccIII TCCGGA 1 cut(s) 228
AciI CCGC 1 cut(s) 33
AclWI GGATC 2 cut(s) 479, 752
AcoI YGGCCR 1 cut(s) 1044
AcsI RAATTY 2 cut(s) 541, 776
AcuI CTGAAG 1 cut(s) 921
AfiI CCNNNNNNNGG 2 cut(s) 202, 411
AflIII ACRYGT 1 cut(s) 234
AgsI TTSAA 9 cut(s) 74, 272, 283, 307, 319, 661, 790, 847, 929
AhlI ACTAGT 1 cut(s) 703
AjnI CCWGG 5 cut(s) 293, 328, 397, 709, 1143
AjuI GAANNNNNNNTTGG 2 cut(s) 818, 850
AloI GAACNNNNNNTCC 2 cut(s) 1064, 1096
AluBI AGCT 8 cut(s) 51, 80, 127, 247, 346, 624, 932, 968
AluI AGCT 8 cut(s) 51, 80, 127, 247, 346, 624, 932, 968
Alw21I GWGCWC 2 cut(s) 129, 626
AlwI GGATC 2 cut(s) 479, 752
Ama87I CYCGRG 1 cut(s) 690
Aor13HI TCCGGA 1 cut(s) 228
AoxI GGCC 2 cut(s) 505, 1044
ApeKI GCWGC 4 cut(s) 132, 389, 741, 998
ApoI RAATTY 2 cut(s) 541, 776
Asp700I GAANNNNTTC 2 cut(s) 302, 1101
AspS9I GGNCC 3 cut(s) 185, 374, 724
AsuHPI GGTGA 3 cut(s) 106, 618, 901
AsuII TTCGAA 1 cut(s) 1092
AvaI CYCGRG 1 cut(s) 690
AvaII GGWCC 3 cut(s) 185, 374, 724
BaeGI GKGCMC 1 cut(s) 697
BalI TGGCCA 1 cut(s) 1046
BanII GRGCYC 2 cut(s) 129, 626
Bbv12I GWGCWC 2 cut(s) 129, 626
BbvI GCAGC 4 cut(s) 144, 401, 753, 985
BccI CCATC 3 cut(s) 32, 271, 806
BciT130I CCWGG 5 cut(s) 295, 330, 399, 711, 1145
BcuI ACTAGT 1 cut(s) 703
BfaI CTAG 3 cut(s) 347, 509, 704
BfmI CTRYAG 1 cut(s) 130
BfuAI ACCTGC 2 cut(s) 4, 637
BglII AGATCT 1 cut(s) 686
BisI GCNGC 4 cut(s) 133, 390, 742, 999
BlsI GCNGC 4 cut(s) 134, 391, 743, 1000
Bme1390I CCNGG 5 cut(s) 295, 330, 399, 711, 1145
Bme18I GGWCC 3 cut(s) 185, 374, 724
BmeT110I CYCGRG 1 cut(s) 690
BmgT120I GGNCC 3 cut(s) 185, 374, 724
BmiI GGNNCC 4 cut(s) 186, 187, 375, 403
BmrFI CCNGG 5 cut(s) 295, 330, 399, 711, 1145
BmrI ACTGGG 1 cut(s) 955
BmsI GCATC 1 cut(s) 164
BmuI ACTGGG 1 cut(s) 955
Bpu14I TTCGAA 1 cut(s) 1092
BsaJI CCNNGG 3 cut(s) 293, 709, 735
BsaWI WCCGGW 1 cut(s) 228
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 411
Bse1I ACTGG 2 cut(s) 950, 1132
Bse3DI GCAATG 4 cut(s) 384, 399, 760, 1074
BseAI TCCGGA 1 cut(s) 228
BseBI CCWGG 5 cut(s) 295, 330, 399, 711, 1145
BseDI CCNNGG 3 cut(s) 293, 709, 735
BseGI GGATG 2 cut(s) 179, 225
BseLI CCNNNNNNNGG 2 cut(s) 202, 411
BseMI GCAATG 4 cut(s) 384, 399, 760, 1074
BseMII CTCAG 3 cut(s) 468, 509, 674
BseNI ACTGG 2 cut(s) 950, 1132
BseRI GAGGAG 2 cut(s) 137, 518
BseSI GKGCMC 1 cut(s) 697
BseXI GCAGC 4 cut(s) 144, 401, 753, 985
BshFI GGCC 2 cut(s) 507, 1046
BsiHKAI GWGCWC 2 cut(s) 129, 626
BsiHKCI CYCGRG 1 cut(s) 690
BsiSI CCGG 1 cut(s) 229
BslFI GGGAC 5 cut(s) 198, 600, 680, 692, 962
BslI CCNNNNNNNGG 2 cut(s) 202, 411
BsmFI GGGAC 5 cut(s) 198, 600, 680, 692, 962
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 2 cut(s) 507, 1046
BsoBI CYCGRG 1 cut(s) 690
Bsp119I TTCGAA 1 cut(s) 1092
Bsp1286I GDGCHC 3 cut(s) 129, 626, 697
Bsp13I TCCGGA 1 cut(s) 228
Bsp143I GATC 5 cut(s) 471, 686, 757, 891, 1108
Bsp19I CCATGG 1 cut(s) 735
BspACI CCGC 1 cut(s) 33
BspANI GGCC 2 cut(s) 507, 1046
BspCNI CTCAG 3 cut(s) 469, 508, 675
BspEI TCCGGA 1 cut(s) 228
BspLI GGNNCC 4 cut(s) 186, 187, 375, 403
BspMAI CTGCAG 1 cut(s) 134
BspMI ACCTGC 2 cut(s) 4, 637
BspPI GGATC 2 cut(s) 479, 752
BspQI GCTCTTC 1 cut(s) 184
BspT104I TTCGAA 1 cut(s) 1092
BsrDI GCAATG 4 cut(s) 384, 399, 760, 1074
BsrI ACTGG 2 cut(s) 950, 1132
BssECI CCNNGG 3 cut(s) 293, 709, 735
BssMI GATC 5 cut(s) 471, 686, 757, 891, 1108
BssT1I CCWWGG 1 cut(s) 735
Bst2UI CCWGG 5 cut(s) 295, 330, 399, 711, 1145
Bst4CI ACNGT 2 cut(s) 94, 313
Bst6I CTCTTC 2 cut(s) 146, 184
BstAPI GCANNNNNTGC 1 cut(s) 998
BstBI TTCGAA 1 cut(s) 1092
BstC8I GCNNGC 2 cut(s) 115, 1048
BstDEI CTNAG 4 cut(s) 477, 495, 683, 1161
BstDSI CCRYGG 1 cut(s) 735
BstF5I GGATG 2 cut(s) 179, 225
BstKTI GATC 5 cut(s) 474, 689, 760, 894, 1111
BstMBI GATC 5 cut(s) 471, 686, 757, 891, 1108
BstMWI GCNNNNNNNGC 4 cut(s) 39, 48, 750, 998
BstNI CCWGG 5 cut(s) 295, 330, 399, 711, 1145
BstSCI CCNGG 5 cut(s) 293, 328, 397, 709, 1143
BstSFI CTRYAG 1 cut(s) 130
BstSLI GKGCMC 1 cut(s) 697
BstV1I GCAGC 4 cut(s) 144, 401, 753, 985
BstX2I RGATCY 2 cut(s) 471, 686
BstYI RGATCY 2 cut(s) 471, 686
BsuRI GGCC 2 cut(s) 507, 1046
BtgI CCRYGG 1 cut(s) 735
BtgZI GCGATG 1 cut(s) 249
BtsCI GGATG 2 cut(s) 179, 225
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 2 cut(s) 323, 1011
BveI ACCTGC 2 cut(s) 4, 637
Cac8I GCNNGC 2 cut(s) 115, 1048
Cfr13I GGNCC 3 cut(s) 185, 374, 724
CseI GACGC 1 cut(s) 119
CsiI ACCWGGT 1 cut(s) 1143
CviAII CATG 8 cut(s) 25, 217, 466, 503, 516, 736, 802, 1114
DdeI CTNAG 4 cut(s) 477, 495, 683, 1161
DpnI GATC 5 cut(s) 473, 688, 759, 893, 1110
DpnII GATC 5 cut(s) 471, 686, 757, 891, 1108
EaeI YGGCCR 1 cut(s) 1044
Eam1104I CTCTTC 2 cut(s) 146, 184
EarI CTCTTC 2 cut(s) 146, 184
EciI GGCGGA 1 cut(s) 48
Ecl136II GAGCTC 2 cut(s) 127, 624
Eco130I CCWWGG 1 cut(s) 735
Eco24I GRGCYC 2 cut(s) 129, 626
Eco47I GGWCC 3 cut(s) 185, 374, 724
Eco53kI GAGCTC 2 cut(s) 127, 624
Eco57I CTGAAG 1 cut(s) 921
Eco88I CYCGRG 1 cut(s) 690
EcoICRI GAGCTC 2 cut(s) 127, 624
EcoO109I RGGNCCY 2 cut(s) 185, 374
EcoRI GAATTC 1 cut(s) 776
EcoRII CCWGG 5 cut(s) 293, 328, 397, 709, 1143
EcoT14I CCWWGG 1 cut(s) 735
EcoT38I GRGCYC 2 cut(s) 129, 626
ErhI CCWWGG 1 cut(s) 735
FaeI CATG 8 cut(s) 28, 220, 469, 506, 519, 739, 805, 1117
FaqI GGGAC 5 cut(s) 198, 600, 680, 692, 962
FatI CATG 8 cut(s) 24, 216, 465, 502, 515, 735, 801, 1113
Fnu4HI GCNGC 4 cut(s) 133, 390, 742, 999
FokI GGATG 2 cut(s) 186, 212
FriOI GRGCYC 2 cut(s) 129, 626
Fsp4HI GCNGC 4 cut(s) 133, 390, 742, 999
FspBI CTAG 3 cut(s) 347, 509, 704
GluI GCNGC 4 cut(s) 133, 390, 742, 999
HaeIII GGCC 2 cut(s) 507, 1046
HapII CCGG 1 cut(s) 229
HgaI GACGC 1 cut(s) 119
Hin1II CATG 8 cut(s) 28, 220, 469, 506, 519, 739, 805, 1117
HindIII AAGCTT 1 cut(s) 930
HinfI GANTC 2 cut(s) 499, 950
HpaII CCGG 1 cut(s) 229
HphI GGTGA 3 cut(s) 106, 618, 901
Hpy166II GTNNAC 2 cut(s) 109, 487
Hpy188I TCNGA 9 cut(s) 156, 211, 243, 478, 498, 684, 785, 938, 1058
Hpy188III TCNNGA 4 cut(s) 183, 229, 280, 1106
Hpy8I GTNNAC 2 cut(s) 109, 487
Hpy99I CGWCG 2 cut(s) 94, 785
HpyAV CCTTC 5 cut(s) 206, 263, 299, 820, 896
HpyCH4III ACNGT 2 cut(s) 94, 313
HpyCH4IV ACGT 1 cut(s) 236
HpyCH4V TGCA 5 cut(s) 132, 389, 768, 863, 1001
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 48, 750, 998
HpyF3I CTNAG 4 cut(s) 477, 495, 683, 1161
HpySE526I ACGT 1 cut(s) 236
Hsp92II CATG 8 cut(s) 28, 220, 469, 506, 519, 739, 805, 1117
KflI GGGWCCC 1 cut(s) 185
Kpn2I TCCGGA 1 cut(s) 228
Kzo9I GATC 5 cut(s) 471, 686, 757, 891, 1108
LguI GCTCTTC 1 cut(s) 184
LmnI GCTCC 1 cut(s) 124
Lsp1109I GCAGC 4 cut(s) 144, 401, 753, 985
LweI GCATC 1 cut(s) 164
MabI ACCWGGT 1 cut(s) 1143
MaeI CTAG 3 cut(s) 347, 509, 704
MaeII ACGT 1 cut(s) 236
MaeIII GTNAC 3 cut(s) 321, 568, 974
MalI GATC 5 cut(s) 473, 688, 759, 893, 1110
MboI GATC 5 cut(s) 471, 686, 757, 891, 1108
MboII GAAGA 7 cut(s) 11, 62, 133, 171, 365, 444, 1106
MflI RGATCY 2 cut(s) 471, 686
MhlI GDGCHC 3 cut(s) 129, 626, 697
MlsI TGGCCA 1 cut(s) 1046
MluCI AATT 7 cut(s) 521, 541, 656, 776, 847, 1076, 1126
MluNI TGGCCA 1 cut(s) 1046
MlyI GAGTC 1 cut(s) 944
MmeI TCCRAC 2 cut(s) 763, 816
Mox20I TGGCCA 1 cut(s) 1046
MroI TCCGGA 1 cut(s) 228
MroXI GAANNNNTTC 2 cut(s) 302, 1101
MscI TGGCCA 1 cut(s) 1046
MseI TTAA 2 cut(s) 639, 1140
MslI CAYNNNNRTG 2 cut(s) 27, 734
Msp20I TGGCCA 1 cut(s) 1046
MspI CCGG 1 cut(s) 229
MspR9I CCNGG 5 cut(s) 295, 330, 399, 711, 1145
Mva1269I GAATGC 1 cut(s) 275
MvaI CCWGG 5 cut(s) 295, 330, 399, 711, 1145
MwoI GCNNNNNNNGC 4 cut(s) 39, 48, 750, 998
NcoI CCATGG 1 cut(s) 735
NdeII GATC 5 cut(s) 471, 686, 757, 891, 1108
NlaIII CATG 8 cut(s) 28, 220, 469, 506, 519, 739, 805, 1117
NlaIV GGNNCC 4 cut(s) 186, 187, 375, 403
NmuCI GTSAC 2 cut(s) 321, 974
NspV TTCGAA 1 cut(s) 1092
PaqCI CACCTGC 1 cut(s) 637
PciSI GCTCTTC 1 cut(s) 184
PctI GAATGC 1 cut(s) 275
PdmI GAANNNNTTC 2 cut(s) 302, 1101
PfeI GAWTC 1 cut(s) 499
PkrI GCNGC 4 cut(s) 134, 391, 743, 1000
PleI GAGTC 1 cut(s) 944
PpsI GAGTC 1 cut(s) 944
PpuMI RGGWCCY 2 cut(s) 185, 374
Psp124BI GAGCTC 2 cut(s) 129, 626
Psp5II RGGWCCY 2 cut(s) 185, 374
Psp6I CCWGG 5 cut(s) 293, 328, 397, 709, 1143
PspGI CCWGG 5 cut(s) 293, 328, 397, 709, 1143
PspN4I GGNNCC 4 cut(s) 186, 187, 375, 403
PspPI GGNCC 3 cut(s) 185, 374, 724
PspPPI RGGWCCY 2 cut(s) 185, 374
PsrI GAACNNNNNNTAC 1 cut(s) 32
PstI CTGCAG 1 cut(s) 134
PsuI RGATCY 2 cut(s) 471, 686
RseI CAYNNNNRTG 2 cut(s) 27, 734
SacI GAGCTC 2 cut(s) 129, 626
SapI GCTCTTC 1 cut(s) 184
SaqAI TTAA 2 cut(s) 639, 1140
SatI GCNGC 4 cut(s) 133, 390, 742, 999
Sau3AI GATC 5 cut(s) 471, 686, 757, 891, 1108
Sau96I GGNCC 3 cut(s) 185, 374, 724
SchI GAGTC 1 cut(s) 944
ScrFI CCNGG 5 cut(s) 295, 330, 399, 711, 1145
SduI GDGCHC 3 cut(s) 129, 626, 697
SexAI ACCWGGT 1 cut(s) 1143
SfaNI GCATC 1 cut(s) 164
SfcI CTRYAG 1 cut(s) 130
SfuI TTCGAA 1 cut(s) 1092
SinI GGWCC 3 cut(s) 185, 374, 724
SmiMI CAYNNNNRTG 2 cut(s) 27, 734
SpeI ACTAGT 1 cut(s) 703
Sse9I AATT 7 cut(s) 521, 541, 656, 776, 847, 1076, 1126
SsiI CCGC 1 cut(s) 33
SspMI CTAG 3 cut(s) 347, 509, 704
SstI GAGCTC 2 cut(s) 129, 626
StyD4I CCNGG 5 cut(s) 293, 328, 397, 709, 1143
StyI CCWWGG 1 cut(s) 735
TaaI ACNGT 2 cut(s) 94, 313
TaiI ACGT 1 cut(s) 239
TaqI TCGA 4 cut(s) 89, 894, 1092, 1107
TasI AATT 7 cut(s) 521, 541, 656, 776, 847, 1076, 1126
TfiI GAWTC 1 cut(s) 499
Tru1I TTAA 2 cut(s) 639, 1140
Tru9I TTAA 2 cut(s) 639, 1140
TscAI CASTG 2 cut(s) 330, 1018
TseFI GTSAC 2 cut(s) 321, 974
TseI GCWGC 4 cut(s) 132, 389, 741, 998
Tsp45I GTSAC 2 cut(s) 321, 974
TspDTI ATGAA 7 cut(s) 41, 366, 373, 437, 491, 657, 1126
TspRI CASTG 2 cut(s) 330, 1018
VpaK11BI GGWCC 3 cut(s) 185, 374, 724
XapI RAATTY 2 cut(s) 541, 776
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
XmnI GAANNNNTTC 2 cut(s) 302, 1101
XspI CTAG 3 cut(s) 347, 509, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.