Rroxscaffold_2G00105660

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000342
Physical Location & Seq
Forward (+)
29094626 .. 29096713
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00105660.1

Sequence Viewer

Length: 549 bp
ATGAATGGTGGAGATGGAAAAAGAAGTTATGCAAGCAACTCGCTGAATCCAGTTACAGGATGTCTGGTTCTTTCTACGGCAGGCTTTTTCCCAGCAATTCTCTCCACTGCGCACTCTAGTTATTCTCTTATGTGGATCTCTAAGGTTCCAAAAGGTTTGGTAACGAAAGGAGGAGAAGGACTGAACAAGGGGAACATATACATAGCCAAGACAAGCTCACATGTTGTGTTTAACGAATACTTTGAGCAATTCAAAAGGGACTTCACTGTCTTTCTGAGGTCTCGGGCACAAGAGCTGGTCTCGGGAGGTAGTATGGGTTTGATTGAAGAGAAAAAATTGGACACATTTAATATGCCATACTATGAGCCAACAGCGGATGAGGTGAAAGAGGCGATCGAGGCTGAAGGTTCTTTTATTTTACAAAACCTCGAAACTTTTAGAAATGACTGGGACTTTTACATAAAACAAGTTAACTGCGGCTTTGACAAGAAAGCGAGGGCAGCAATACTATCCACTGACATAAGAGCTGTGGGAGAGCCTATTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

182

Amino Acids

20.17

Weight (kDa)

6.15

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 33 - 105 1.1e-24 SAM dependent carboxyl methyltransferase
Methyltransf_7 PF03492 105 - 182 1.7e-15 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14060 AT2G14060 AT3G11480 AT3G21950 AT3G21950 AT5G04370 AT5G04370 AT5G04370 AT5G04380 AT5G04380 AT5G04380 AT5G38020 AT5G38020 AT5G66430
fragaria_vesca FvH4_2g09290 FvH4_2g09290 FvH4_2g09310 FvH4_3g03130
malus_domestica MD10G1071000.v1.1 MD10G1071200.v1.1 MD10G1071500.v1.1 MD10G1218400.v1.1
prunus_persica Prupe.8G092700_v2.0.a1 Prupe.8G092800_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G093000_v2.0.a1 Prupe.8G093100_v2.0.a1 Prupe.8G093200_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093600_v2.0.a1
pyrus_communis pycom10g05660 pycom10g05680 pycom10g18420
rosa_chinensis RchiOBHm_Chr1g0324421 RchiOBHm_Chr1g0324461 RchiOBHm_Chr6g0265311 RchiOBHm_Chr6g0265321 RchiOBHm_Chr6g0265361 RchiOBHm_Chr6g0265391 RchiOBHm_Chr6g0265411
rosa_laevigata RLG00000014186 RLG00000014191 RLG00000014195 RLG00000014197 RLG00000025381 RLG00000030199
rosa_multiflora Rmu_co8237181.1_g000001 Rmu_sc0001780.1_g000008 Rmu_sc0001803.1_g000020 Rmu_sc0002550.1_g000004 Rmu_sc0004082.1_g000012 Rmu_sc0008601.1_g000002 Rmu_sc0011954.1_g000001 Rmu_sc0013000.1_g000001 Rmu_sc0016482.1_g000001 Rmu_sc0018713.1_g000002 Rmu_ssc0000066.1_g000015
rosa_roxburghii Rroxscaffold_159G00432720 Rroxscaffold_159G00432790 Rroxscaffold_159G00432850 Rroxscaffold_175G00432320 Rroxscaffold_175G00432340 Rroxscaffold_175G00432350 Rroxscaffold_175G00432360 Rroxscaffold_2G00105600 Rroxscaffold_2G00105660 Rroxscaffold_4G00325530 Rroxscaffold_4G00325600 Rroxscaffold_4G00325670 Rroxscaffold_6G00424510 Rroxscaffold_7G00202680 Rroxscaffold_7G00202760 Rroxscaffold_7G00202800 Rroxscaffold_7G00202850
rosa_rugosa Rorug01G0045900 Rorug01G0046000 Rorug02G0355000 Rorug02G0355300 Rorug03G0004200 Rorug06G0016700 Rorug06G0016700 Rorug06G0016800 Rorug06G0017100
rosa_samantha Rh1AG061600 Rh1AG061700 Rh1BG052400 Rh1CG063500 Rh1CG063900 Rh1CG064100 Rh2BG415800 Rh3DG067100 Rh6AG138200 Rh6AG138400 Rh6AG138800 Rh6BG137100 Rh6BG137200 Rh6BG137600 Rh6DG121600 Rh6DG121700 Rh6DG121900
rosa_wichuraiana Rw1G005230 Rw1G005470 Rw2G033110 Rw6G011920 Rw6G011940 Rw6G011950 Rw6G011970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 266
Acc16I TGCGCA 1 cut(s) 111
AciI CCGC 2 cut(s) 374, 477
AclWI GGATC 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 423
AfiI CCNNNNNNNGG 1 cut(s) 56
AflIII ACRYGT 1 cut(s) 220
AgsI TTSAA 2 cut(s) 253, 326
AloI GAACNNNNNNTCC 2 cut(s) 51, 83
AluBI AGCT 3 cut(s) 216, 295, 527
AluI AGCT 3 cut(s) 216, 295, 527
Alw26I GTCTC 2 cut(s) 285, 304
AlwI GGATC 1 cut(s) 143
Ama87I CYCGRG 2 cut(s) 282, 301
ApeKI GCWGC 1 cut(s) 500
AspLEI GCGC 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 394
AvaI CYCGRG 2 cut(s) 282, 301
BaeGI GKGCMC 1 cut(s) 289
BbvI GCAGC 1 cut(s) 512
BccI CCATC 1 cut(s) 8
BceAI ACGGC 1 cut(s) 93
BcgI CGANNNNNNTGC 2 cut(s) 21, 55
BcoDI GTCTC 2 cut(s) 285, 304
BfaI CTAG 2 cut(s) 117, 547
BisI GCNGC 2 cut(s) 478, 501
BlsI GCNGC 2 cut(s) 479, 502
BmeT110I CYCGRG 2 cut(s) 282, 301
BmiI GGNNCC 1 cut(s) 147
BmrI ACTGGG 1 cut(s) 457
BmuI ACTGGG 1 cut(s) 457
BplI GAGNNNNNCTC 2 cut(s) 284, 316
BsaI GGTCTC 2 cut(s) 285, 304
Bsc4I CCNNNNNNNGG 1 cut(s) 56
Bse1I ACTGG 2 cut(s) 50, 452
BseGI GGATG 2 cut(s) 65, 382
BseLI CCNNNNNNNGG 1 cut(s) 56
BseMII CTCAG 1 cut(s) 266
BseNI ACTGG 2 cut(s) 50, 452
BseRI GAGGAG 1 cut(s) 186
BseSI GKGCMC 1 cut(s) 289
BseXI GCAGC 1 cut(s) 512
BseYI CCCAGC 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 396
BsiEI CGRYCG 1 cut(s) 396
BsiHKCI CYCGRG 2 cut(s) 282, 301
BslFI GGGAC 2 cut(s) 272, 464
BslI CCNNNNNNNGG 1 cut(s) 56
BsmAI GTCTC 2 cut(s) 285, 304
BsmFI GGGAC 2 cut(s) 272, 464
Bso31I GGTCTC 2 cut(s) 285, 304
BsoBI CYCGRG 2 cut(s) 282, 301
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 2 cut(s) 135, 393
BspACI CCGC 2 cut(s) 374, 477
BspCNI CTCAG 1 cut(s) 267
BspLI GGNNCC 1 cut(s) 147
BspPI GGATC 1 cut(s) 143
BspTNI GGTCTC 2 cut(s) 285, 304
BsrI ACTGG 2 cut(s) 50, 452
BssMI GATC 2 cut(s) 135, 393
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 321
BstC8I GCNNGC 2 cut(s) 34, 82
BstDEI CTNAG 2 cut(s) 141, 275
BstF5I GGATG 2 cut(s) 65, 382
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 2 cut(s) 138, 396
BstMAI GTCTC 2 cut(s) 285, 304
BstMBI GATC 2 cut(s) 135, 393
BstMCI CGRYCG 1 cut(s) 396
BstMWI GCNNNNNNNGC 2 cut(s) 398, 500
BstNSI RCATGY 1 cut(s) 224
BstSLI GKGCMC 1 cut(s) 289
BstV1I GCAGC 1 cut(s) 512
BstX2I RGATCY 1 cut(s) 135
BstYI RGATCY 1 cut(s) 135
BtsCI GGATG 2 cut(s) 65, 382
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 3 cut(s) 105, 264, 513
Cac8I GCNNGC 2 cut(s) 34, 82
CfoI GCGC 1 cut(s) 112
CviAII CATG 1 cut(s) 221
CviJI RGCY 9 cut(s) 84, 206, 216, 295, 367, 401, 480, 527, 538
CviKI_1 RGCY 9 cut(s) 84, 206, 216, 295, 367, 401, 480, 527, 538
DdeI CTNAG 2 cut(s) 141, 275
DpnI GATC 2 cut(s) 137, 395
DpnII GATC 2 cut(s) 135, 393
DrdI GACNNNNNNGTC 1 cut(s) 266
DseDI GACNNNNNNGTC 1 cut(s) 266
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
Eco31I GGTCTC 2 cut(s) 285, 304
Eco57I CTGAAG 1 cut(s) 423
Eco88I CYCGRG 2 cut(s) 282, 301
FaeI CATG 1 cut(s) 224
FaqI GGGAC 2 cut(s) 272, 464
FatI CATG 1 cut(s) 220
Fnu4HI GCNGC 2 cut(s) 478, 501
FokI GGATG 2 cut(s) 72, 389
Fsp4HI GCNGC 2 cut(s) 478, 501
FspBI CTAG 2 cut(s) 117, 547
FspI TGCGCA 1 cut(s) 111
GlaI GCGC 1 cut(s) 111
GluI GCNGC 2 cut(s) 478, 501
GsaI CCCAGC 1 cut(s) 95
HhaI GCGC 1 cut(s) 112
Hin1II CATG 1 cut(s) 224
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HinfI GANTC 1 cut(s) 46
HpaI GTTAAC 1 cut(s) 472
HphI GGTGA 1 cut(s) 394
Hpy166II GTNNAC 1 cut(s) 472
Hpy188I TCNGA 1 cut(s) 276
Hpy188III TCNNGA 1 cut(s) 303
Hpy8I GTNNAC 1 cut(s) 472
HpyAV CCTTC 2 cut(s) 170, 398
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4V TGCA 1 cut(s) 32
HpyF10VI GCNNNNNNNGC 2 cut(s) 398, 500
HpyF3I CTNAG 2 cut(s) 141, 275
Hsp92II CATG 1 cut(s) 224
HspAI GCGC 1 cut(s) 110
KspAI GTTAAC 1 cut(s) 472
Kzo9I GATC 2 cut(s) 135, 393
LpnPI CCDG 7 cut(s) 42, 50, 63, 66, 105, 281, 433
Lsp1109I GCAGC 1 cut(s) 512
MaeI CTAG 2 cut(s) 117, 547
MaeIII GTNAC 2 cut(s) 52, 160
MalI GATC 2 cut(s) 137, 395
MboI GATC 2 cut(s) 135, 393
MboII GAAGA 1 cut(s) 338
MflI RGATCY 1 cut(s) 135
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 3 cut(s) 96, 248, 335
MnlI CCTC 8 cut(s) 164, 270, 299, 373, 382, 391, 437, 489
MseI TTAA 3 cut(s) 231, 348, 471
MspA1I CMGCKG 1 cut(s) 374
MwoI GCNNNNNNNGC 2 cut(s) 398, 500
NdeII GATC 2 cut(s) 135, 393
NlaIII CATG 1 cut(s) 224
NlaIV GGNNCC 1 cut(s) 147
NsbI TGCGCA 1 cut(s) 111
NspI RCATGY 1 cut(s) 224
PciI ACATGT 1 cut(s) 220
PfeI GAWTC 1 cut(s) 46
PkrI GCNGC 2 cut(s) 479, 502
Ple19I CGATCG 1 cut(s) 396
PscI ACATGT 1 cut(s) 220
PspFI CCCAGC 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 147
PsuI RGATCY 1 cut(s) 135
PvuI CGATCG 1 cut(s) 396
SaqAI TTAA 3 cut(s) 231, 348, 471
SatI GCNGC 2 cut(s) 478, 501
Sau3AI GATC 2 cut(s) 135, 393
SduI GDGCHC 1 cut(s) 289
Sse9I AATT 3 cut(s) 96, 248, 335
SsiI CCGC 2 cut(s) 374, 477
SspMI CTAG 2 cut(s) 117, 547
TaaI ACNGT 1 cut(s) 268
TaqI TCGA 2 cut(s) 396, 429
TasI AATT 3 cut(s) 96, 248, 335
TauI GCSGC 1 cut(s) 480
TfiI GAWTC 1 cut(s) 46
Tru1I TTAA 3 cut(s) 231, 348, 471
Tru9I TTAA 3 cut(s) 231, 348, 471
TscAI CASTG 3 cut(s) 112, 271, 520
TseI GCWGC 1 cut(s) 500
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 112, 271, 520
XceI RCATGY 1 cut(s) 224
XspI CTAG 2 cut(s) 117, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.