Rh6DG121900

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000342
Physical Location & Seq
Forward (+)
17101179 .. 17103475
2297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG121900.1

Sequence Viewer

Length: 1101 bp
ATGGAGGTAGAGCAGGTTCTTCACATGAATGGCGGAGAAGGCAAAACAAGCTACGCAAACCATTCACTTCTTCAAAGAGCTGTGATTTCGACGGTGAAGCCCATAGTGGACGCAAGCATAGAGGAGCTCTGCAGCACTCTCTTCCCAGAGTGTCTGAAAATAGCAGACTTGGGATGCTCTTCGGGACCCAATACCCTTCTGGTGGTATCAGACATCATGGACAACATCCAGAACACGTTTCGGAAGCTGAACCTTCCCCCACCATCGCTTCAAGCATTCTTGAATGACCTTCCCAGGAACGATTTCAACACGGTGTTCAAGTCACTGCCTGGCTTCTATAAGAAGCTCGATGAAGAACCTGATAAAAAGTCGGGTCCTTGTTTCATTGCAGCAATGCCTGGTTCCTTTTATGGGAGGCTCTTTCCTAACAACTCTCTCCACTTTGTTCATTCTTCTTATGCTCTCATGTGGATCTCTGAGGTTCCAAAAGGTTTGGTAACAAAAGCAGGAGAGGGACTGAACAAGGGGAACATATACATAGCCAAGACGAGCTCACCTGCTGTGTTTAATGAATACTTTGAGCAATTCAAAAGGGACTTCACACTCTTTCTGAGATCTCGGGCACAAGAACTAGTCCCAGGAGGTAGTATGGTCCTCACAACCATGGGCAGCATAACGAGCAATGATCCCCTCTGCATTTGGGAATTCGTCGGATTGAAACTCAATGACATGGTTTTAGATGGTTTGATTGAGAAGGAAATGTTGGACATATTCAATTTGCCATACTATGCACCCACAAGATATGAGGTAAAGGAGGTGATCGAGGCTGAAGGGTCTTTTACTTTGCAAAACCTTGAAGCTTTCAGAAATGACTGGGACTCTTACATAAAACAAGCTAAAAGTGACCTTGACAAGAAAGCAAGGGCTGCAATACTCTCCACTGACATAAGGGCTGTGGGAGAGCCTATTCTGGCCAGCCATTTCGGAGAGGAAGCAATGGACGAATTGTTCAGTAGGTTCGAAGAAAATGTTCTCGATTACATGGAAAGAGAGAAGTGCCAGTTCATTAACCTGGTTATCTCATTGACTAAGAAGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

366

Amino Acids

41.21

Weight (kDa)

5.13

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 40 - 364 5e-122 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14060 AT2G14060 AT3G11480 AT3G21950 AT3G21950 AT5G04370 AT5G04370 AT5G04370 AT5G04380 AT5G04380 AT5G04380 AT5G38020 AT5G38020 AT5G66430
fragaria_vesca FvH4_2g09290 FvH4_2g09290 FvH4_2g09310 FvH4_3g03130
malus_domestica MD10G1071000.v1.1 MD10G1071200.v1.1 MD10G1071500.v1.1 MD10G1218400.v1.1
prunus_persica Prupe.8G092700_v2.0.a1 Prupe.8G092800_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G093000_v2.0.a1 Prupe.8G093100_v2.0.a1 Prupe.8G093200_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093600_v2.0.a1
pyrus_communis pycom10g05660 pycom10g05680 pycom10g18420
rosa_chinensis RchiOBHm_Chr1g0324421 RchiOBHm_Chr1g0324461 RchiOBHm_Chr6g0265311 RchiOBHm_Chr6g0265321 RchiOBHm_Chr6g0265361 RchiOBHm_Chr6g0265391 RchiOBHm_Chr6g0265411
rosa_laevigata RLG00000014186 RLG00000014191 RLG00000014195 RLG00000014197 RLG00000025381 RLG00000030199
rosa_multiflora Rmu_co8237181.1_g000001 Rmu_sc0001780.1_g000008 Rmu_sc0001803.1_g000020 Rmu_sc0002550.1_g000004 Rmu_sc0004082.1_g000012 Rmu_sc0008601.1_g000002 Rmu_sc0011954.1_g000001 Rmu_sc0013000.1_g000001 Rmu_sc0016482.1_g000001 Rmu_sc0018713.1_g000002 Rmu_ssc0000066.1_g000015
rosa_roxburghii Rroxscaffold_159G00432720 Rroxscaffold_159G00432790 Rroxscaffold_159G00432850 Rroxscaffold_175G00432320 Rroxscaffold_175G00432340 Rroxscaffold_175G00432350 Rroxscaffold_175G00432360 Rroxscaffold_2G00105600 Rroxscaffold_2G00105660 Rroxscaffold_4G00325530 Rroxscaffold_4G00325600 Rroxscaffold_4G00325670 Rroxscaffold_6G00424510 Rroxscaffold_7G00202680 Rroxscaffold_7G00202760 Rroxscaffold_7G00202800 Rroxscaffold_7G00202850
rosa_rugosa Rorug01G0045900 Rorug01G0046000 Rorug02G0355000 Rorug02G0355300 Rorug03G0004200 Rorug06G0016700 Rorug06G0016700 Rorug06G0016800 Rorug06G0017100
rosa_samantha Rh1AG061600 Rh1AG061700 Rh1BG052400 Rh1CG063500 Rh1CG063900 Rh1CG064100 Rh2BG415800 Rh3DG067100 Rh6AG138200 Rh6AG138400 Rh6AG138800 Rh6BG137100 Rh6BG137200 Rh6BG137600 Rh6DG121600 Rh6DG121700 Rh6DG121900
rosa_wichuraiana Rw1G005230 Rw1G005470 Rw2G033110 Rw6G011920 Rw6G011940 Rw6G011950 Rw6G011970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 565
Acc36I ACCTGC 2 cut(s) 4, 565
AciI CCGC 1 cut(s) 33
AclWI GGATC 2 cut(s) 479, 680
AcoI YGGCCR 1 cut(s) 972
AcsI RAATTY 1 cut(s) 704
AcuI CTGAAG 1 cut(s) 849
AfiI CCNNNNNNNGG 2 cut(s) 202, 411
AflIII ACRYGT 1 cut(s) 234
AgsI TTSAA 9 cut(s) 74, 272, 283, 307, 319, 589, 718, 775, 857
AhlI ACTAGT 1 cut(s) 631
AjnI CCWGG 5 cut(s) 293, 328, 397, 637, 1071
AjuI GAANNNNNNNTTGG 2 cut(s) 746, 778
AloI GAACNNNNNNTCC 2 cut(s) 992, 1024
AluBI AGCT 8 cut(s) 51, 80, 127, 247, 346, 552, 860, 896
AluI AGCT 8 cut(s) 51, 80, 127, 247, 346, 552, 860, 896
Alw21I GWGCWC 2 cut(s) 129, 554
AlwI GGATC 2 cut(s) 479, 680
Ama87I CYCGRG 1 cut(s) 618
AoxI GGCC 1 cut(s) 972
ApeKI GCWGC 4 cut(s) 132, 389, 669, 926
ApoI RAATTY 1 cut(s) 704
Asp700I GAANNNNTTC 2 cut(s) 302, 1029
AspS9I GGNCC 3 cut(s) 185, 374, 652
AsuHPI GGTGA 3 cut(s) 106, 546, 829
AsuII TTCGAA 1 cut(s) 1020
AvaI CYCGRG 1 cut(s) 618
AvaII GGWCC 3 cut(s) 185, 374, 652
BaeGI GKGCMC 1 cut(s) 625
BalI TGGCCA 1 cut(s) 974
BanII GRGCYC 2 cut(s) 129, 554
Bbv12I GWGCWC 2 cut(s) 129, 554
BbvI GCAGC 4 cut(s) 144, 401, 681, 913
BccI CCATC 2 cut(s) 271, 734
BciT130I CCWGG 5 cut(s) 295, 330, 399, 639, 1073
BcuI ACTAGT 1 cut(s) 631
BfaI CTAG 1 cut(s) 632
BfmI CTRYAG 1 cut(s) 130
BfuAI ACCTGC 2 cut(s) 4, 565
BglII AGATCT 1 cut(s) 614
BisI GCNGC 4 cut(s) 133, 390, 670, 927
BlsI GCNGC 4 cut(s) 134, 391, 671, 928
Bme1390I CCNGG 5 cut(s) 295, 330, 399, 639, 1073
Bme18I GGWCC 3 cut(s) 185, 374, 652
BmeT110I CYCGRG 1 cut(s) 618
BmgT120I GGNCC 3 cut(s) 185, 374, 652
BmiI GGNNCC 5 cut(s) 186, 187, 375, 403, 483
BmrFI CCNGG 5 cut(s) 295, 330, 399, 639, 1073
BmrI ACTGGG 1 cut(s) 883
BmsI GCATC 1 cut(s) 164
BmuI ACTGGG 1 cut(s) 883
Bpu14I TTCGAA 1 cut(s) 1020
BsaJI CCNNGG 3 cut(s) 293, 637, 663
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 411
Bse1I ACTGG 2 cut(s) 878, 1060
Bse3DI GCAATG 4 cut(s) 384, 399, 688, 1002
BseBI CCWGG 5 cut(s) 295, 330, 399, 639, 1073
BseDI CCNNGG 3 cut(s) 293, 637, 663
BseGI GGATG 2 cut(s) 179, 225
BseLI CCNNNNNNNGG 2 cut(s) 202, 411
BseMI GCAATG 4 cut(s) 384, 399, 688, 1002
BseMII CTCAG 2 cut(s) 468, 602
BseNI ACTGG 2 cut(s) 878, 1060
BseRI GAGGAG 1 cut(s) 137
BseSI GKGCMC 1 cut(s) 625
BseXI GCAGC 4 cut(s) 144, 401, 681, 913
BshFI GGCC 1 cut(s) 974
BsiHKAI GWGCWC 2 cut(s) 129, 554
BsiHKCI CYCGRG 1 cut(s) 618
BslFI GGGAC 5 cut(s) 198, 528, 608, 620, 890
BslI CCNNNNNNNGG 2 cut(s) 202, 411
BsmFI GGGAC 5 cut(s) 198, 528, 608, 620, 890
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 1 cut(s) 974
BsoBI CYCGRG 1 cut(s) 618
Bsp119I TTCGAA 1 cut(s) 1020
Bsp1286I GDGCHC 3 cut(s) 129, 554, 625
Bsp143I GATC 4 cut(s) 471, 614, 685, 819
Bsp19I CCATGG 1 cut(s) 663
BspACI CCGC 1 cut(s) 33
BspANI GGCC 1 cut(s) 974
BspCNI CTCAG 2 cut(s) 469, 603
BspLI GGNNCC 5 cut(s) 186, 187, 375, 403, 483
BspMAI CTGCAG 1 cut(s) 134
BspMI ACCTGC 2 cut(s) 4, 565
BspPI GGATC 2 cut(s) 479, 680
BspQI GCTCTTC 1 cut(s) 184
BspT104I TTCGAA 1 cut(s) 1020
BsrDI GCAATG 4 cut(s) 384, 399, 688, 1002
BsrI ACTGG 2 cut(s) 878, 1060
BssECI CCNNGG 3 cut(s) 293, 637, 663
BssMI GATC 4 cut(s) 471, 614, 685, 819
BssT1I CCWWGG 1 cut(s) 663
Bst2UI CCWGG 5 cut(s) 295, 330, 399, 639, 1073
Bst4CI ACNGT 2 cut(s) 94, 313
Bst6I CTCTTC 2 cut(s) 146, 184
BstAPI GCANNNNNTGC 1 cut(s) 926
BstBI TTCGAA 1 cut(s) 1020
BstC8I GCNNGC 2 cut(s) 115, 976
BstDEI CTNAG 3 cut(s) 477, 611, 1089
BstDSI CCRYGG 1 cut(s) 663
BstF5I GGATG 2 cut(s) 179, 225
BstKTI GATC 4 cut(s) 474, 617, 688, 822
BstMBI GATC 4 cut(s) 471, 614, 685, 819
BstMWI GCNNNNNNNGC 4 cut(s) 39, 48, 678, 926
BstNI CCWGG 5 cut(s) 295, 330, 399, 639, 1073
BstSCI CCNGG 5 cut(s) 293, 328, 397, 637, 1071
BstSFI CTRYAG 1 cut(s) 130
BstSLI GKGCMC 1 cut(s) 625
BstV1I GCAGC 4 cut(s) 144, 401, 681, 913
BstX2I RGATCY 2 cut(s) 471, 614
BstYI RGATCY 2 cut(s) 471, 614
BsuRI GGCC 1 cut(s) 974
BtgI CCRYGG 1 cut(s) 663
BtgZI GCGATG 1 cut(s) 249
BtsCI GGATG 2 cut(s) 179, 225
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 2 cut(s) 323, 939
BveI ACCTGC 2 cut(s) 4, 565
Cac8I GCNNGC 2 cut(s) 115, 976
Cfr13I GGNCC 3 cut(s) 185, 374, 652
CseI GACGC 1 cut(s) 119
CsiI ACCWGGT 1 cut(s) 1071
CviAII CATG 6 cut(s) 25, 217, 466, 664, 730, 1042
DdeI CTNAG 3 cut(s) 477, 611, 1089
DpnI GATC 4 cut(s) 473, 616, 687, 821
DpnII GATC 4 cut(s) 471, 614, 685, 819
EaeI YGGCCR 1 cut(s) 972
Eam1104I CTCTTC 2 cut(s) 146, 184
EarI CTCTTC 2 cut(s) 146, 184
EciI GGCGGA 1 cut(s) 48
Ecl136II GAGCTC 2 cut(s) 127, 552
Eco130I CCWWGG 1 cut(s) 663
Eco24I GRGCYC 2 cut(s) 129, 554
Eco47I GGWCC 3 cut(s) 185, 374, 652
Eco53kI GAGCTC 2 cut(s) 127, 552
Eco57I CTGAAG 1 cut(s) 849
Eco88I CYCGRG 1 cut(s) 618
EcoICRI GAGCTC 2 cut(s) 127, 552
EcoO109I RGGNCCY 2 cut(s) 185, 374
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 5 cut(s) 293, 328, 397, 637, 1071
EcoT14I CCWWGG 1 cut(s) 663
EcoT38I GRGCYC 2 cut(s) 129, 554
ErhI CCWWGG 1 cut(s) 663
FaeI CATG 6 cut(s) 28, 220, 469, 667, 733, 1045
FaqI GGGAC 5 cut(s) 198, 528, 608, 620, 890
FatI CATG 6 cut(s) 24, 216, 465, 663, 729, 1041
Fnu4HI GCNGC 4 cut(s) 133, 390, 670, 927
FokI GGATG 2 cut(s) 186, 212
FriOI GRGCYC 2 cut(s) 129, 554
Fsp4HI GCNGC 4 cut(s) 133, 390, 670, 927
FspBI CTAG 1 cut(s) 632
GluI GCNGC 4 cut(s) 133, 390, 670, 927
HaeIII GGCC 1 cut(s) 974
HgaI GACGC 1 cut(s) 119
Hin1II CATG 6 cut(s) 28, 220, 469, 667, 733, 1045
HindIII AAGCTT 1 cut(s) 858
HinfI GANTC 1 cut(s) 878
HphI GGTGA 3 cut(s) 106, 546, 829
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 8 cut(s) 156, 211, 243, 478, 612, 713, 866, 986
Hpy188III TCNNGA 4 cut(s) 183, 229, 280, 1034
Hpy8I GTNNAC 1 cut(s) 109
Hpy99I CGWCG 2 cut(s) 94, 713
HpyAV CCTTC 6 cut(s) 32, 206, 263, 299, 748, 824
HpyCH4III ACNGT 2 cut(s) 94, 313
HpyCH4IV ACGT 1 cut(s) 236
HpyCH4V TGCA 6 cut(s) 132, 389, 696, 791, 847, 929
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 48, 678, 926
HpyF3I CTNAG 3 cut(s) 477, 611, 1089
HpySE526I ACGT 1 cut(s) 236
Hsp92II CATG 6 cut(s) 28, 220, 469, 667, 733, 1045
KflI GGGWCCC 1 cut(s) 185
Kzo9I GATC 4 cut(s) 471, 614, 685, 819
LguI GCTCTTC 1 cut(s) 184
LmnI GCTCC 1 cut(s) 124
Lsp1109I GCAGC 4 cut(s) 144, 401, 681, 913
LweI GCATC 1 cut(s) 164
MabI ACCWGGT 1 cut(s) 1071
MaeI CTAG 1 cut(s) 632
MaeII ACGT 1 cut(s) 236
MaeIII GTNAC 3 cut(s) 321, 496, 902
MalI GATC 4 cut(s) 473, 616, 687, 821
MboI GATC 4 cut(s) 471, 614, 685, 819
MboII GAAGA 7 cut(s) 11, 62, 133, 171, 365, 444, 1034
MflI RGATCY 2 cut(s) 471, 614
MhlI GDGCHC 3 cut(s) 129, 554, 625
MlsI TGGCCA 1 cut(s) 974
MluCI AATT 4 cut(s) 584, 704, 775, 1004
MluNI TGGCCA 1 cut(s) 974
MlyI GAGTC 1 cut(s) 872
MmeI TCCRAC 2 cut(s) 691, 744
Mox20I TGGCCA 1 cut(s) 974
MroXI GAANNNNTTC 2 cut(s) 302, 1029
MscI TGGCCA 1 cut(s) 974
MseI TTAA 2 cut(s) 567, 1068
MslI CAYNNNNRTG 2 cut(s) 27, 662
Msp20I TGGCCA 1 cut(s) 974
MspR9I CCNGG 5 cut(s) 295, 330, 399, 639, 1073
Mva1269I GAATGC 1 cut(s) 275
MvaI CCWGG 5 cut(s) 295, 330, 399, 639, 1073
MwoI GCNNNNNNNGC 4 cut(s) 39, 48, 678, 926
NcoI CCATGG 1 cut(s) 663
NdeII GATC 4 cut(s) 471, 614, 685, 819
NlaIII CATG 6 cut(s) 28, 220, 469, 667, 733, 1045
NlaIV GGNNCC 5 cut(s) 186, 187, 375, 403, 483
NmuCI GTSAC 2 cut(s) 321, 902
NspV TTCGAA 1 cut(s) 1020
PaqCI CACCTGC 1 cut(s) 565
PciSI GCTCTTC 1 cut(s) 184
PctI GAATGC 1 cut(s) 275
PdmI GAANNNNTTC 2 cut(s) 302, 1029
PkrI GCNGC 4 cut(s) 134, 391, 671, 928
PleI GAGTC 1 cut(s) 872
PpsI GAGTC 1 cut(s) 872
PpuMI RGGWCCY 2 cut(s) 185, 374
Psp124BI GAGCTC 2 cut(s) 129, 554
Psp5II RGGWCCY 2 cut(s) 185, 374
Psp6I CCWGG 5 cut(s) 293, 328, 397, 637, 1071
PspGI CCWGG 5 cut(s) 293, 328, 397, 637, 1071
PspN4I GGNNCC 5 cut(s) 186, 187, 375, 403, 483
PspPI GGNCC 3 cut(s) 185, 374, 652
PspPPI RGGWCCY 2 cut(s) 185, 374
PsrI GAACNNNNNNTAC 1 cut(s) 32
PstI CTGCAG 1 cut(s) 134
PsuI RGATCY 2 cut(s) 471, 614
RseI CAYNNNNRTG 2 cut(s) 27, 662
SacI GAGCTC 2 cut(s) 129, 554
SapI GCTCTTC 1 cut(s) 184
SaqAI TTAA 2 cut(s) 567, 1068
SatI GCNGC 4 cut(s) 133, 390, 670, 927
Sau3AI GATC 4 cut(s) 471, 614, 685, 819
Sau96I GGNCC 3 cut(s) 185, 374, 652
SchI GAGTC 1 cut(s) 872
ScrFI CCNGG 5 cut(s) 295, 330, 399, 639, 1073
SduI GDGCHC 3 cut(s) 129, 554, 625
SexAI ACCWGGT 1 cut(s) 1071
SfaNI GCATC 1 cut(s) 164
SfcI CTRYAG 1 cut(s) 130
SfuI TTCGAA 1 cut(s) 1020
SinI GGWCC 3 cut(s) 185, 374, 652
SmiMI CAYNNNNRTG 2 cut(s) 27, 662
SpeI ACTAGT 1 cut(s) 631
Sse9I AATT 4 cut(s) 584, 704, 775, 1004
SsiI CCGC 1 cut(s) 33
SspMI CTAG 1 cut(s) 632
SstI GAGCTC 2 cut(s) 129, 554
StyD4I CCNGG 5 cut(s) 293, 328, 397, 637, 1071
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 2 cut(s) 94, 313
TaiI ACGT 1 cut(s) 239
TaqI TCGA 5 cut(s) 89, 348, 822, 1020, 1035
TasI AATT 4 cut(s) 584, 704, 775, 1004
Tru1I TTAA 2 cut(s) 567, 1068
Tru9I TTAA 2 cut(s) 567, 1068
TscAI CASTG 2 cut(s) 330, 946
TseFI GTSAC 2 cut(s) 321, 902
TseI GCWGC 4 cut(s) 132, 389, 669, 926
Tsp45I GTSAC 2 cut(s) 321, 902
TspDTI ATGAA 6 cut(s) 41, 366, 373, 437, 585, 1054
TspRI CASTG 2 cut(s) 330, 946
VpaK11BI GGWCC 3 cut(s) 185, 374, 652
XapI RAATTY 1 cut(s) 704
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
XmnI GAANNNNTTC 2 cut(s) 302, 1029
XspI CTAG 1 cut(s) 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.