Rroxscaffold_6G00424510

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000342
Physical Location & Seq
Reverse (-)
45633746 .. 45634706
961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00424510.1

Sequence Viewer

Length: 663 bp
ATGTACGTAGTTATTCCCAGCTTTGATCATATATTCATATTGGTGGAATGCCTGCAGGTTCCAAAAGGTTTGGTAACAGAAGGATTCAACGAGGGAAACATATGCATAGCCAAGACAAGCGCACCTTCTGCGTTTAATGAATACTTTGAACAATTTAAAAACGACTTCACAGTGTTTCTAAGGTCTCGTGCAGAAGAGTTGGTCCCTCAGGGTGGAATGGTCCTTACAATCAGGGGCAGCATAAACAGTCATGAACCCCACTCTATATTCGAATGTCTTGGATTGAAAATCAATGATATGGTTTTAGAGGGTTTGATTGAAAGGAAAAACTTGGACACCTTCAATATTCTATACTACGAACCTACAATAGAGGAACTGATGGAGCTGATCGAGGCCGAAGGATCCTTTAGGTTGCAGGACCTTCAAGTTTTTAAACATGATTGGGACTCTTTTATAAAGGAAGCTGATAGTGGTCTCGATAAGAAAGCTATATTCTCCACTCACATTAGGTCTGTGGTGGAGCCTTTTCTGGTCAGTCACTTTGGAGAGGGAGTCATGAAAGATTTATTTCGCAGGTTCGAAAAAGATGTTCTTGATTACATGGAAAAGGAGAAGTGCCGGTCTATCGATATGGTTTTTTTTTTTTTTTCCCTTCTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

220

Amino Acids

25.53

Weight (kDa)

4.85

Isoelectric Point (pI)

59.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 19 - 213 3.2e-50 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14060 AT2G14060 AT3G11480 AT3G21950 AT3G21950 AT5G04370 AT5G04370 AT5G04370 AT5G04380 AT5G04380 AT5G04380 AT5G38020 AT5G38020 AT5G66430
fragaria_vesca FvH4_2g09290 FvH4_2g09290 FvH4_2g09310 FvH4_3g03130
malus_domestica MD10G1071000.v1.1 MD10G1071200.v1.1 MD10G1071500.v1.1 MD10G1218400.v1.1
prunus_persica Prupe.8G092700_v2.0.a1 Prupe.8G092800_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G093000_v2.0.a1 Prupe.8G093100_v2.0.a1 Prupe.8G093200_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093600_v2.0.a1
pyrus_communis pycom10g05660 pycom10g05680 pycom10g18420
rosa_chinensis RchiOBHm_Chr1g0324421 RchiOBHm_Chr1g0324461 RchiOBHm_Chr6g0265311 RchiOBHm_Chr6g0265321 RchiOBHm_Chr6g0265361 RchiOBHm_Chr6g0265391 RchiOBHm_Chr6g0265411
rosa_laevigata RLG00000014186 RLG00000014191 RLG00000014195 RLG00000014197 RLG00000025381 RLG00000030199
rosa_multiflora Rmu_co8237181.1_g000001 Rmu_sc0001780.1_g000008 Rmu_sc0001803.1_g000020 Rmu_sc0002550.1_g000004 Rmu_sc0004082.1_g000012 Rmu_sc0008601.1_g000002 Rmu_sc0011954.1_g000001 Rmu_sc0013000.1_g000001 Rmu_sc0016482.1_g000001 Rmu_sc0018713.1_g000002 Rmu_ssc0000066.1_g000015
rosa_roxburghii Rroxscaffold_159G00432720 Rroxscaffold_159G00432790 Rroxscaffold_159G00432850 Rroxscaffold_175G00432320 Rroxscaffold_175G00432340 Rroxscaffold_175G00432350 Rroxscaffold_175G00432360 Rroxscaffold_2G00105600 Rroxscaffold_2G00105660 Rroxscaffold_4G00325530 Rroxscaffold_4G00325600 Rroxscaffold_4G00325670 Rroxscaffold_6G00424510 Rroxscaffold_7G00202680 Rroxscaffold_7G00202760 Rroxscaffold_7G00202800 Rroxscaffold_7G00202850
rosa_rugosa Rorug01G0045900 Rorug01G0046000 Rorug02G0355000 Rorug02G0355300 Rorug03G0004200 Rorug06G0016700 Rorug06G0016700 Rorug06G0016800 Rorug06G0017100
rosa_samantha Rh1AG061600 Rh1AG061700 Rh1BG052400 Rh1CG063500 Rh1CG063900 Rh1CG064100 Rh2BG415800 Rh3DG067100 Rh6AG138200 Rh6AG138400 Rh6AG138800 Rh6BG137100 Rh6BG137200 Rh6BG137600 Rh6DG121600 Rh6DG121700 Rh6DG121900
rosa_wichuraiana Rw1G005230 Rw1G005470 Rw2G033110 Rw6G011920 Rw6G011940 Rw6G011950 Rw6G011970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 455
Acc36I ACCTGC 2 cut(s) 46, 564
AclWI GGATC 2 cut(s) 396, 409
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 212
AgsI TTSAA 6 cut(s) 88, 149, 286, 320, 343, 425
AluBI AGCT 4 cut(s) 21, 385, 464, 488
AluI AGCT 4 cut(s) 21, 385, 464, 488
Alw26I GTCTC 2 cut(s) 189, 479
AlwI GGATC 2 cut(s) 396, 409
AoxI GGCC 1 cut(s) 393
ApeKI GCWGC 1 cut(s) 237
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 3 cut(s) 202, 220, 418
AsuII TTCGAA 2 cut(s) 270, 579
AvaII GGWCC 3 cut(s) 202, 220, 418
AxyI CCTNAGG 1 cut(s) 207
BamHI GGATCC 1 cut(s) 401
BauI CACGAG 1 cut(s) 186
BbvI GCAGC 1 cut(s) 249
BccI CCATC 1 cut(s) 373
BclI TGATCA 1 cut(s) 25
BcoDI GTCTC 2 cut(s) 189, 479
BfmI CTRYAG 1 cut(s) 53
BfuAI ACCTGC 2 cut(s) 46, 564
BisI GCNGC 1 cut(s) 238
BlsI GCNGC 1 cut(s) 239
Bme18I GGWCC 3 cut(s) 202, 220, 418
BmgT120I GGNCC 3 cut(s) 202, 220, 418
BmiI GGNNCC 4 cut(s) 60, 204, 403, 522
Bpu14I TTCGAA 2 cut(s) 270, 579
Bsa29I ATCGAT 1 cut(s) 627
BsaAI YACGTR 1 cut(s) 7
BsaI GGTCTC 2 cut(s) 189, 479
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 1 cut(s) 212
Bse118I RCCGGY 1 cut(s) 618
Bse21I CCTNAGG 1 cut(s) 207
BseCI ATCGAT 1 cut(s) 627
BseLI CCNNNNNNNGG 1 cut(s) 212
BseMII CTCAG 1 cut(s) 221
BseXI GCAGC 1 cut(s) 249
BseYI CCCAGC 1 cut(s) 17
BsgI GTGCAG 1 cut(s) 210
BshFI GGCC 1 cut(s) 395
BshVI ATCGAT 1 cut(s) 627
BsiSI CCGG 1 cut(s) 619
BslFI GGGAC 2 cut(s) 188, 458
BslI CCNNNNNNNGG 1 cut(s) 212
BsmAI GTCTC 2 cut(s) 189, 479
BsmFI GGGAC 2 cut(s) 188, 458
BsmI GAATGC 1 cut(s) 53
BsnI GGCC 1 cut(s) 395
Bso31I GGTCTC 2 cut(s) 189, 479
Bsp119I TTCGAA 2 cut(s) 270, 579
Bsp143I GATC 3 cut(s) 25, 387, 401
BspANI GGCC 1 cut(s) 395
BspCNI CTCAG 1 cut(s) 220
BspDI ATCGAT 1 cut(s) 627
BspHI TCATGA 2 cut(s) 250, 555
BspLI GGNNCC 4 cut(s) 60, 204, 403, 522
BspMAI CTGCAG 1 cut(s) 57
BspMI ACCTGC 2 cut(s) 46, 564
BspPI GGATC 2 cut(s) 396, 409
BspT104I TTCGAA 2 cut(s) 270, 579
BspTNI GGTCTC 2 cut(s) 189, 479
BsrFI RCCGGY 1 cut(s) 618
BssAI RCCGGY 1 cut(s) 618
BssMI GATC 3 cut(s) 25, 387, 401
BssSI CACGAG 1 cut(s) 186
Bst2BI CACGAG 1 cut(s) 186
Bst4CI ACNGT 2 cut(s) 172, 248
Bst6I CTCTTC 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 128
BstBAI YACGTR 1 cut(s) 7
BstBI TTCGAA 2 cut(s) 270, 579
BstC8I GCNNGC 1 cut(s) 53
BstDEI CTNAG 2 cut(s) 179, 207
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 3 cut(s) 28, 390, 404
BstMAI GTCTC 2 cut(s) 189, 479
BstMBI GATC 3 cut(s) 25, 387, 401
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstSFI CTRYAG 1 cut(s) 53
BstSNI TACGTA 1 cut(s) 7
BstV1I GCAGC 1 cut(s) 249
BstX2I RGATCY 1 cut(s) 401
BstYI RGATCY 1 cut(s) 401
Bsu15I ATCGAT 1 cut(s) 627
Bsu36I CCTNAGG 1 cut(s) 207
BsuRI GGCC 1 cut(s) 395
BsuTUI ATCGAT 1 cut(s) 627
BtsIMutI CAGTG 1 cut(s) 177
BveI ACCTGC 2 cut(s) 46, 564
Cac8I GCNNGC 1 cut(s) 53
CciI TCATGA 2 cut(s) 250, 555
CfoI GCGC 1 cut(s) 122
Cfr10I RCCGGY 1 cut(s) 618
Cfr13I GGNCC 3 cut(s) 202, 220, 418
ClaI ATCGAT 1 cut(s) 627
Csp6I GTAC 1 cut(s) 4
CviAII CATG 4 cut(s) 251, 437, 556, 601
CviJI RGCY 7 cut(s) 21, 110, 385, 395, 464, 488, 523
CviKI_1 RGCY 7 cut(s) 21, 110, 385, 395, 464, 488, 523
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 2 cut(s) 179, 207
DpnI GATC 3 cut(s) 27, 389, 403
DpnII GATC 3 cut(s) 25, 387, 401
DraI TTTAAA 2 cut(s) 157, 433
Eam1104I CTCTTC 1 cut(s) 189
EarI CTCTTC 1 cut(s) 189
Eco105I TACGTA 1 cut(s) 7
Eco31I GGTCTC 2 cut(s) 189, 479
Eco47I GGWCC 3 cut(s) 202, 220, 418
Eco81I CCTNAGG 1 cut(s) 207
EcoO109I RGGNCCY 1 cut(s) 418
EcoT22I ATGCAT 1 cut(s) 107
FaeI CATG 4 cut(s) 254, 440, 559, 604
FalI AAGNNNNNCTT 4 cut(s) 109, 141, 576, 608
FaqI GGGAC 2 cut(s) 188, 458
FatI CATG 4 cut(s) 250, 436, 555, 600
FauNDI CATATG 1 cut(s) 101
FbaI TGATCA 1 cut(s) 25
Fnu4HI GCNGC 1 cut(s) 238
Fsp4HI GCNGC 1 cut(s) 238
GlaI GCGC 1 cut(s) 121
GluI GCNGC 1 cut(s) 238
GsaI CCCAGC 1 cut(s) 21
HaeIII GGCC 1 cut(s) 395
HapII CCGG 1 cut(s) 619
HhaI GCGC 1 cut(s) 122
Hin1II CATG 4 cut(s) 254, 440, 559, 604
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HinfI GANTC 3 cut(s) 84, 446, 552
HpaII CCGG 1 cut(s) 619
Hpy188III TCNNGA 4 cut(s) 251, 476, 556, 593
HpyAV CCTTC 6 cut(s) 74, 135, 349, 392, 431, 662
HpyCH4III ACNGT 2 cut(s) 172, 248
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 4 cut(s) 55, 105, 191, 415
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 2 cut(s) 179, 207
HpySE526I ACGT 1 cut(s) 6
Hsp92II CATG 4 cut(s) 254, 440, 559, 604
HspAI GCGC 1 cut(s) 120
Ksp22I TGATCA 1 cut(s) 25
Kzo9I GATC 3 cut(s) 25, 387, 401
LmnI GCTCC 2 cut(s) 382, 520
LpnPI CCDG 9 cut(s) 31, 41, 65, 194, 217, 401, 515, 559, 632
Lsp1109I GCAGC 1 cut(s) 249
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 2 cut(s) 73, 536
MalI GATC 3 cut(s) 27, 389, 403
MboI GATC 3 cut(s) 25, 387, 401
MboII GAAGA 1 cut(s) 206
MflI RGATCY 1 cut(s) 401
MluCI AATT 1 cut(s) 152
MlyI GAGTC 2 cut(s) 440, 561
MnlI CCTC 6 cut(s) 85, 216, 301, 364, 385, 541
Mph1103I ATGCAT 1 cut(s) 107
MseI TTAA 4 cut(s) 135, 156, 432, 661
MslI CAYNNNNRTG 1 cut(s) 41
MspI CCGG 1 cut(s) 619
Mva1269I GAATGC 1 cut(s) 53
MwoI GCNNNNNNNGC 1 cut(s) 128
NdeI CATATG 1 cut(s) 101
NdeII GATC 3 cut(s) 25, 387, 401
NlaIII CATG 4 cut(s) 254, 440, 559, 604
NlaIV GGNNCC 4 cut(s) 60, 204, 403, 522
NmuCI GTSAC 1 cut(s) 536
NsiI ATGCAT 1 cut(s) 107
NspV TTCGAA 2 cut(s) 270, 579
PagI TCATGA 2 cut(s) 250, 555
PctI GAATGC 1 cut(s) 53
PfeI GAWTC 1 cut(s) 84
PkrI GCNGC 1 cut(s) 239
PleI GAGTC 2 cut(s) 440, 560
PpsI GAGTC 2 cut(s) 440, 560
Ppu21I YACGTR 1 cut(s) 7
PpuMI RGGWCCY 1 cut(s) 418
PsiI TTATAA 1 cut(s) 455
Psp5II RGGWCCY 1 cut(s) 418
PspFI CCCAGC 1 cut(s) 17
PspN4I GGNNCC 4 cut(s) 60, 204, 403, 522
PspPI GGNCC 3 cut(s) 202, 220, 418
PspPPI RGGWCCY 1 cut(s) 418
PstI CTGCAG 1 cut(s) 57
PsuI RGATCY 1 cut(s) 401
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 1 cut(s) 41
SaqAI TTAA 4 cut(s) 135, 156, 432, 661
SatI GCNGC 1 cut(s) 238
Sau3AI GATC 3 cut(s) 25, 387, 401
Sau96I GGNCC 3 cut(s) 202, 220, 418
SbfI CCTGCAGG 1 cut(s) 57
SchI GAGTC 2 cut(s) 440, 561
SdaI CCTGCAGG 1 cut(s) 57
SfcI CTRYAG 1 cut(s) 53
SfuI TTCGAA 2 cut(s) 270, 579
SinI GGWCC 3 cut(s) 202, 220, 418
SmiMI CAYNNNNRTG 1 cut(s) 41
SnaBI TACGTA 1 cut(s) 7
Sse8387I CCTGCAGG 1 cut(s) 57
Sse9I AATT 1 cut(s) 152
SspI AATATT 1 cut(s) 346
TaaI ACNGT 2 cut(s) 172, 248
TaiI ACGT 1 cut(s) 9
TaqI TCGA 5 cut(s) 270, 390, 477, 579, 627
TasI AATT 1 cut(s) 152
TfiI GAWTC 1 cut(s) 84
Tru1I TTAA 4 cut(s) 135, 156, 432, 661
Tru9I TTAA 4 cut(s) 135, 156, 432, 661
TscAI CASTG 1 cut(s) 177
TseFI GTSAC 1 cut(s) 536
TseI GCWGC 1 cut(s) 237
Tsp45I GTSAC 1 cut(s) 536
TspDTI ATGAA 4 cut(s) 25, 153, 267, 572
TspRI CASTG 1 cut(s) 177
VpaK11BI GGWCC 3 cut(s) 202, 220, 418
Zsp2I ATGCAT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.