FvH4_2g09310

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000342
Physical Location & Seq
Forward (+)
8237529 .. 8238640
1112 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g09310.t1

Sequence Viewer

Length: 708 bp
ATGCCTGGTTCCTTTTATGGCAGGCTCTTTCCTGACAACTCTCTTCACTTTGTTCATTCTTCTTATGCTCTCATGTGGATCTCTGAGGTTCCAAAAGGTTTGGTAACCAAAGGAGGAGAGGGACTGAACAAGGGGAACATATACATAGCCAAGACAAGCTCACCTGCTGTGTTTAATGAATATTTTGAACAATTCAAAAGGGACTTCACAGTCTTTCTGCGATCTAGGGCACACGAGCTAGTCCCGGGAGGTAGTATGGTCCTTACAACCATGGGCAGCATAAAGAGCAATGATCCCCTCTCCATTTGGGAATTCGTCGGATTGAAACTCCATGACATGGTTTTAGATGGTTTGATTGAGGAGAAAATGTTGGACAGATTCAATTTGCCATACTATGCACCCACAACAGATGAGGTAAAAGAGGTGATCGAGGCTGAAGGGTCTTTTACTTTGCAAAACCTTGAATCTTTCAGAAATGACTGGGATTCTTACATAAAACAAGCTCATAGTGACCTTGACAAGAAAGCAAGGGCTGCAATACTCTCCACTGACATAAGGGCAGTGGGAGAGCCTATTCTGGCTAGCCAATTTGGAGAAGAAGCTGTGGACGAATTGTTCCGTAGGTTCGAAGAAAATGTTATTGATCATATGGAAAGGGAGAAGTGTCAGTTCATTAACCTAGTTATATCATTGATCAAGAAGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

236

Amino Acids

26.82

Weight (kDa)

5.33

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 1 - 233 1.5e-80 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14060 AT2G14060 AT3G11480 AT3G21950 AT3G21950 AT5G04370 AT5G04370 AT5G04370 AT5G04380 AT5G04380 AT5G04380 AT5G38020 AT5G38020 AT5G66430
fragaria_vesca FvH4_2g09290 FvH4_2g09290 FvH4_2g09310 FvH4_3g03130
malus_domestica MD10G1071000.v1.1 MD10G1071200.v1.1 MD10G1071500.v1.1 MD10G1218400.v1.1
prunus_persica Prupe.8G092700_v2.0.a1 Prupe.8G092800_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G093000_v2.0.a1 Prupe.8G093100_v2.0.a1 Prupe.8G093200_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093600_v2.0.a1
pyrus_communis pycom10g05660 pycom10g05680 pycom10g18420
rosa_chinensis RchiOBHm_Chr1g0324421 RchiOBHm_Chr1g0324461 RchiOBHm_Chr6g0265311 RchiOBHm_Chr6g0265321 RchiOBHm_Chr6g0265361 RchiOBHm_Chr6g0265391 RchiOBHm_Chr6g0265411
rosa_laevigata RLG00000014186 RLG00000014191 RLG00000014195 RLG00000014197 RLG00000025381 RLG00000030199
rosa_multiflora Rmu_co8237181.1_g000001 Rmu_sc0001780.1_g000008 Rmu_sc0001803.1_g000020 Rmu_sc0002550.1_g000004 Rmu_sc0004082.1_g000012 Rmu_sc0008601.1_g000002 Rmu_sc0011954.1_g000001 Rmu_sc0013000.1_g000001 Rmu_sc0016482.1_g000001 Rmu_sc0018713.1_g000002 Rmu_ssc0000066.1_g000015
rosa_roxburghii Rroxscaffold_159G00432720 Rroxscaffold_159G00432790 Rroxscaffold_159G00432850 Rroxscaffold_175G00432320 Rroxscaffold_175G00432340 Rroxscaffold_175G00432350 Rroxscaffold_175G00432360 Rroxscaffold_2G00105600 Rroxscaffold_2G00105660 Rroxscaffold_4G00325530 Rroxscaffold_4G00325600 Rroxscaffold_4G00325670 Rroxscaffold_6G00424510 Rroxscaffold_7G00202680 Rroxscaffold_7G00202760 Rroxscaffold_7G00202800 Rroxscaffold_7G00202850
rosa_rugosa Rorug01G0045900 Rorug01G0046000 Rorug02G0355000 Rorug02G0355300 Rorug03G0004200 Rorug06G0016700 Rorug06G0016700 Rorug06G0016800 Rorug06G0017100
rosa_samantha Rh1AG061600 Rh1AG061700 Rh1BG052400 Rh1CG063500 Rh1CG063900 Rh1CG064100 Rh2BG415800 Rh3DG067100 Rh6AG138200 Rh6AG138400 Rh6AG138800 Rh6BG137100 Rh6BG137200 Rh6BG137600 Rh6DG121600 Rh6DG121700 Rh6DG121900
rosa_wichuraiana Rw1G005230 Rw1G005470 Rw2G033110 Rw6G011920 Rw6G011940 Rw6G011950 Rw6G011970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 172
AasI GACNNNNNNGTC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 172
AccB7I CCANNNNNTGG 1 cut(s) 337
AclWI GGATC 2 cut(s) 86, 287
AcsI RAATTY 1 cut(s) 311
AcuI CTGAAG 1 cut(s) 456
AfiI CCNNNNNNNGG 1 cut(s) 337
AgsI TTSAA 5 cut(s) 188, 196, 325, 382, 464
AjnI CCWGG 1 cut(s) 4
AloI GAACNNNNNNTCC 2 cut(s) 599, 631
AluBI AGCT 4 cut(s) 159, 238, 503, 602
AluI AGCT 4 cut(s) 159, 238, 503, 602
AlwI GGATC 2 cut(s) 86, 287
Ama87I CYCGRG 1 cut(s) 244
ApeKI GCWGC 2 cut(s) 276, 533
ApoI RAATTY 1 cut(s) 311
AspS9I GGNCC 1 cut(s) 259
AsuC2I CCSGG 2 cut(s) 245, 246
AsuHPI GGTGA 2 cut(s) 153, 436
AsuII TTCGAA 1 cut(s) 627
AsuNHI GCTAGC 1 cut(s) 581
AvaI CYCGRG 1 cut(s) 244
AvaII GGWCC 1 cut(s) 259
BaeGI GKGCMC 1 cut(s) 232
BauI CACGAG 1 cut(s) 233
BbvI GCAGC 2 cut(s) 288, 520
BccI CCATC 1 cut(s) 341
BciT130I CCWGG 1 cut(s) 6
BclI TGATCA 2 cut(s) 643, 693
BcnI CCSGG 2 cut(s) 245, 246
BfaI CTAG 4 cut(s) 225, 239, 582, 680
BfuAI ACCTGC 1 cut(s) 172
BisI GCNGC 2 cut(s) 277, 534
BlsI GCNGC 2 cut(s) 278, 535
Bme1390I CCNGG 3 cut(s) 6, 245, 246
Bme18I GGWCC 1 cut(s) 259
BmeT110I CYCGRG 1 cut(s) 244
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 2 cut(s) 10, 90
BmrFI CCNGG 3 cut(s) 6, 245, 246
BmrI ACTGGG 1 cut(s) 490
BmtI GCTAGC 1 cut(s) 585
BmuI ACTGGG 1 cut(s) 490
Bpu14I TTCGAA 1 cut(s) 627
BpuMI CCSGG 2 cut(s) 245, 246
BsaJI CCNNGG 2 cut(s) 244, 270
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 1 cut(s) 337
Bse1I ACTGG 1 cut(s) 485
Bse3DI GCAATG 1 cut(s) 295
BseBI CCWGG 1 cut(s) 6
BseDI CCNNGG 2 cut(s) 244, 270
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMI GCAATG 1 cut(s) 295
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 485
BseRI GAGGAG 2 cut(s) 129, 374
BseSI GKGCMC 1 cut(s) 232
BseXI GCAGC 2 cut(s) 288, 520
BsiHKCI CYCGRG 1 cut(s) 244
BsiSI CCGG 1 cut(s) 245
BslFI GGGAC 3 cut(s) 135, 215, 227
BslI CCNNNNNNNGG 1 cut(s) 337
BsmFI GGGAC 3 cut(s) 135, 215, 227
BsoBI CYCGRG 1 cut(s) 244
Bsp119I TTCGAA 1 cut(s) 627
Bsp1286I GDGCHC 1 cut(s) 232
Bsp143I GATC 6 cut(s) 78, 221, 292, 426, 643, 693
Bsp19I CCATGG 1 cut(s) 270
BspCNI CTCAG 1 cut(s) 76
BspLI GGNNCC 2 cut(s) 10, 90
BspMI ACCTGC 1 cut(s) 172
BspOI GCTAGC 1 cut(s) 585
BspPI GGATC 2 cut(s) 86, 287
BspT104I TTCGAA 1 cut(s) 627
BsrDI GCAATG 1 cut(s) 295
BsrI ACTGG 1 cut(s) 485
BssECI CCNNGG 2 cut(s) 244, 270
BssMI GATC 6 cut(s) 78, 221, 292, 426, 643, 693
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 270
Bst2BI CACGAG 1 cut(s) 233
Bst2UI CCWGG 1 cut(s) 6
Bst4CI ACNGT 1 cut(s) 211
Bst6I CTCTTC 1 cut(s) 48
BstAPI GCANNNNNTGC 1 cut(s) 533
BstBI TTCGAA 1 cut(s) 627
BstC8I GCNNGC 2 cut(s) 23, 583
BstDEI CTNAG 1 cut(s) 84
BstDSI CCRYGG 1 cut(s) 270
BstEII GGTNACC 1 cut(s) 103
BstKTI GATC 6 cut(s) 81, 224, 295, 429, 646, 696
BstMBI GATC 6 cut(s) 78, 221, 292, 426, 643, 693
BstMWI GCNNNNNNNGC 2 cut(s) 285, 533
BstNI CCWGG 1 cut(s) 6
BstPI GGTNACC 1 cut(s) 103
BstSCI CCNGG 3 cut(s) 4, 243, 244
BstSLI GKGCMC 1 cut(s) 232
BstV1I GCAGC 2 cut(s) 288, 520
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
BtgI CCRYGG 1 cut(s) 270
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 2 cut(s) 546, 567
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 2 cut(s) 23, 583
Cfr13I GGNCC 1 cut(s) 259
Cfr9I CCCGGG 1 cut(s) 244
CviAII CATG 4 cut(s) 73, 271, 332, 337
DdeI CTNAG 1 cut(s) 84
DpnI GATC 6 cut(s) 80, 223, 294, 428, 645, 695
DpnII GATC 6 cut(s) 78, 221, 292, 426, 643, 693
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 259
Eco57I CTGAAG 1 cut(s) 456
Eco88I CYCGRG 1 cut(s) 244
Eco91I GGTNACC 1 cut(s) 103
EcoO65I GGTNACC 1 cut(s) 103
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 4
EcoT14I CCWWGG 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 270
FaeI CATG 4 cut(s) 76, 274, 335, 340
FaqI GGGAC 3 cut(s) 135, 215, 227
FatI CATG 4 cut(s) 72, 270, 331, 336
FauNDI CATATG 1 cut(s) 648
FbaI TGATCA 2 cut(s) 643, 693
Fnu4HI GCNGC 2 cut(s) 277, 534
Fsp4HI GCNGC 2 cut(s) 277, 534
FspBI CTAG 4 cut(s) 225, 239, 582, 680
GluI GCNGC 2 cut(s) 277, 534
HapII CCGG 1 cut(s) 245
Hin1II CATG 4 cut(s) 76, 274, 335, 340
HinfI GANTC 3 cut(s) 378, 464, 485
HpaII CCGG 1 cut(s) 245
HphI GGTGA 2 cut(s) 153, 436
Hpy166II GTNNAC 1 cut(s) 607
Hpy188I TCNGA 3 cut(s) 85, 320, 473
Hpy188III TCNNGA 2 cut(s) 32, 697
Hpy8I GTNNAC 1 cut(s) 607
Hpy99I CGWCG 1 cut(s) 320
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4V TGCA 3 cut(s) 398, 454, 536
HpyF10VI GCNNNNNNNGC 2 cut(s) 285, 533
HpyF3I CTNAG 1 cut(s) 84
Hsp92II CATG 4 cut(s) 76, 274, 335, 340
Ksp22I TGATCA 2 cut(s) 643, 693
Kzo9I GATC 6 cut(s) 78, 221, 292, 426, 643, 693
LpnPI CCDG 7 cut(s) 7, 18, 45, 177, 258, 466, 563
Lsp1109I GCAGC 2 cut(s) 288, 520
MaeI CTAG 4 cut(s) 225, 239, 582, 680
MaeIII GTNAC 2 cut(s) 103, 509
MalI GATC 6 cut(s) 80, 223, 294, 428, 645, 695
MboI GATC 6 cut(s) 78, 221, 292, 426, 643, 693
MboII GAAGA 4 cut(s) 35, 51, 608, 641
MflI RGATCY 1 cut(s) 78
MhlI GDGCHC 1 cut(s) 232
MluCI AATT 5 cut(s) 191, 311, 382, 587, 611
MmeI TCCRAC 2 cut(s) 298, 351
MnlI CCTC 9 cut(s) 79, 107, 112, 242, 308, 352, 406, 415, 424
MseI TTAA 2 cut(s) 174, 675
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 3 cut(s) 6, 245, 246
MvaI CCWGG 1 cut(s) 6
MwoI GCNNNNNNNGC 2 cut(s) 285, 533
NciI CCSGG 2 cut(s) 245, 246
NcoI CCATGG 1 cut(s) 270
NdeI CATATG 1 cut(s) 648
NdeII GATC 6 cut(s) 78, 221, 292, 426, 643, 693
NheI GCTAGC 1 cut(s) 581
NlaIII CATG 4 cut(s) 76, 274, 335, 340
NlaIV GGNNCC 2 cut(s) 10, 90
NmuCI GTSAC 1 cut(s) 509
NspV TTCGAA 1 cut(s) 627
PaqCI CACCTGC 1 cut(s) 172
PfeI GAWTC 3 cut(s) 378, 464, 485
PflMI CCANNNNNTGG 1 cut(s) 337
PkrI GCNGC 2 cut(s) 278, 535
Psp6I CCWGG 1 cut(s) 4
PspEI GGTNACC 1 cut(s) 103
PspGI CCWGG 1 cut(s) 4
PspN4I GGNNCC 2 cut(s) 10, 90
PspPI GGNCC 1 cut(s) 259
PsuI RGATCY 1 cut(s) 78
SaqAI TTAA 2 cut(s) 174, 675
SatI GCNGC 2 cut(s) 277, 534
Sau3AI GATC 6 cut(s) 78, 221, 292, 426, 643, 693
Sau96I GGNCC 1 cut(s) 259
ScrFI CCNGG 3 cut(s) 6, 245, 246
SduI GDGCHC 1 cut(s) 232
SfuI TTCGAA 1 cut(s) 627
SinI GGWCC 1 cut(s) 259
SmaI CCCGGG 1 cut(s) 246
Sse9I AATT 5 cut(s) 191, 311, 382, 587, 611
SspI AATATT 1 cut(s) 182
SspMI CTAG 4 cut(s) 225, 239, 582, 680
StyD4I CCNGG 3 cut(s) 4, 243, 244
StyI CCWWGG 1 cut(s) 270
TaaI ACNGT 1 cut(s) 211
TaqI TCGA 2 cut(s) 429, 627
TasI AATT 5 cut(s) 191, 311, 382, 587, 611
TfiI GAWTC 3 cut(s) 378, 464, 485
Tru1I TTAA 2 cut(s) 174, 675
Tru9I TTAA 2 cut(s) 174, 675
TscAI CASTG 2 cut(s) 553, 567
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 2 cut(s) 276, 533
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 3 cut(s) 44, 192, 661
TspGWI ACGGA 1 cut(s) 608
TspMI CCCGGG 1 cut(s) 244
TspRI CASTG 2 cut(s) 553, 567
Van91I CCANNNNNTGG 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 259
XapI RAATTY 1 cut(s) 311
XmaI CCCGGG 1 cut(s) 244
XspI CTAG 4 cut(s) 225, 239, 582, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.