MD10G1218400.v1.1

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000342
Physical Location & Seq
Forward (+)
31643332 .. 31643742
411 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1218400.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGTCATCACAACATTGGGGAGCACAAAGAGCCATGATCCACTTAGCATTTGGGAAGTTGTCGGACTCAAACTCAATAATATGGTTTTAGAGGGTTTGATTGAGGAGGAAAAATTGGACACATTCAATATGCCATTGTATTTTCCTACAACAAAGGAGGCTGAAGATGTCATCGAGGAGGAAGGATTTTTTACTTTGCAAAGCCTTGAAGTTTTCAAAAATGATTGGGACTCCTATATAAAGCATGCTGACAGTGGCCTCGATAAAAAGGCAAGGGCTGCCGTACTTGCCACTGAGATAAGGGCTGTGGTAGAGCCTCTTCTGGCAACCCAATTCGGAGAAGTGGCTATGGACGATTTGTTTCGCAGGTTTGAAGAAGATGTCCTTGATCACATGGAAATGGAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

137

Amino Acids

15.54

Weight (kDa)

4.34

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 1 - 134 3.2e-32 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14060 AT2G14060 AT3G11480 AT3G21950 AT3G21950 AT5G04370 AT5G04370 AT5G04370 AT5G04380 AT5G04380 AT5G04380 AT5G38020 AT5G38020 AT5G66430
fragaria_vesca FvH4_2g09290 FvH4_2g09290 FvH4_2g09310 FvH4_3g03130
malus_domestica MD10G1071000.v1.1 MD10G1071200.v1.1 MD10G1071500.v1.1 MD10G1218400.v1.1
prunus_persica Prupe.8G092700_v2.0.a1 Prupe.8G092800_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G093000_v2.0.a1 Prupe.8G093100_v2.0.a1 Prupe.8G093200_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093600_v2.0.a1
pyrus_communis pycom10g05660 pycom10g05680 pycom10g18420
rosa_chinensis RchiOBHm_Chr1g0324421 RchiOBHm_Chr1g0324461 RchiOBHm_Chr6g0265311 RchiOBHm_Chr6g0265321 RchiOBHm_Chr6g0265361 RchiOBHm_Chr6g0265391 RchiOBHm_Chr6g0265411
rosa_laevigata RLG00000014186 RLG00000014191 RLG00000014195 RLG00000014197 RLG00000025381 RLG00000030199
rosa_multiflora Rmu_co8237181.1_g000001 Rmu_sc0001780.1_g000008 Rmu_sc0001803.1_g000020 Rmu_sc0002550.1_g000004 Rmu_sc0004082.1_g000012 Rmu_sc0008601.1_g000002 Rmu_sc0011954.1_g000001 Rmu_sc0013000.1_g000001 Rmu_sc0016482.1_g000001 Rmu_sc0018713.1_g000002 Rmu_ssc0000066.1_g000015
rosa_roxburghii Rroxscaffold_159G00432720 Rroxscaffold_159G00432790 Rroxscaffold_159G00432850 Rroxscaffold_175G00432320 Rroxscaffold_175G00432340 Rroxscaffold_175G00432350 Rroxscaffold_175G00432360 Rroxscaffold_2G00105600 Rroxscaffold_2G00105660 Rroxscaffold_4G00325530 Rroxscaffold_4G00325600 Rroxscaffold_4G00325670 Rroxscaffold_6G00424510 Rroxscaffold_7G00202680 Rroxscaffold_7G00202760 Rroxscaffold_7G00202800 Rroxscaffold_7G00202850
rosa_rugosa Rorug01G0045900 Rorug01G0046000 Rorug02G0355000 Rorug02G0355300 Rorug03G0004200 Rorug06G0016700 Rorug06G0016700 Rorug06G0016800 Rorug06G0017100
rosa_samantha Rh1AG061600 Rh1AG061700 Rh1BG052400 Rh1CG063500 Rh1CG063900 Rh1CG064100 Rh2BG415800 Rh3DG067100 Rh6AG138200 Rh6AG138400 Rh6AG138800 Rh6BG137100 Rh6BG137200 Rh6BG137600 Rh6DG121600 Rh6DG121700 Rh6DG121900
rosa_wichuraiana Rw1G005230 Rw1G005470 Rw2G033110 Rw6G011920 Rw6G011940 Rw6G011950 Rw6G011970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 357
AclWI GGATC 1 cut(s) 32
AcuI CTGAAG 1 cut(s) 183
AfaI GTAC 1 cut(s) 285
AgsI TTSAA 4 cut(s) 127, 209, 217, 374
Alw21I GWGCWC 1 cut(s) 26
AlwI GGATC 1 cut(s) 32
AoxI GGCC 1 cut(s) 256
ApeKI GCWGC 1 cut(s) 278
BarI GAAGNNNNNNTAC 2 cut(s) 303, 335
Bbv12I GWGCWC 1 cut(s) 26
BbvI GCAGC 1 cut(s) 265
BceAI ACGGC 1 cut(s) 266
BclI TGATCA 1 cut(s) 388
BfuAI ACCTGC 1 cut(s) 357
BisI GCNGC 1 cut(s) 279
BlsI GCNGC 1 cut(s) 280
BseMII CTCAG 1 cut(s) 285
BseRI GAGGAG 2 cut(s) 119, 191
BseXI GCAGC 1 cut(s) 265
BshFI GGCC 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 26
BslFI GGGAC 1 cut(s) 242
BsmFI GGGAC 1 cut(s) 242
BsnI GGCC 1 cut(s) 258
Bsp1286I GDGCHC 1 cut(s) 26
Bsp143I GATC 2 cut(s) 37, 388
BspANI GGCC 1 cut(s) 258
BspCNI CTCAG 1 cut(s) 286
BspMI ACCTGC 1 cut(s) 357
BspPI GGATC 1 cut(s) 32
BssMI GATC 2 cut(s) 37, 388
Bst4CI ACNGT 1 cut(s) 254
Bst6I CTCTTC 1 cut(s) 324
BstAPI GCANNNNNTGC 1 cut(s) 278
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 2 cut(s) 44, 294
BstKTI GATC 2 cut(s) 40, 391
BstMBI GATC 2 cut(s) 37, 388
BstMWI GCNNNNNNNGC 3 cut(s) 30, 278, 287
BstNSI RCATGY 1 cut(s) 248
BstV1I GCAGC 1 cut(s) 265
BsuRI GGCC 1 cut(s) 258
BtsIMutI CAGTG 2 cut(s) 259, 291
BveI ACCTGC 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 246
Csp6I GTAC 1 cut(s) 284
CviAII CATG 3 cut(s) 35, 245, 394
CviJI RGCY 8 cut(s) 33, 161, 204, 258, 278, 305, 316, 347
CviKI_1 RGCY 8 cut(s) 33, 161, 204, 258, 278, 305, 316, 347
CviQI GTAC 1 cut(s) 284
DdeI CTNAG 2 cut(s) 44, 294
DpnI GATC 2 cut(s) 39, 390
DpnII GATC 2 cut(s) 37, 388
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Eco57I CTGAAG 1 cut(s) 183
FaeI CATG 3 cut(s) 38, 248, 397
FaiI YATR 8 cut(s) 36, 83, 131, 237, 239, 246, 350, 395
FalI AAGNNNNNCTT 2 cut(s) 369, 401
FaqI GGGAC 1 cut(s) 242
FatI CATG 3 cut(s) 34, 244, 393
FbaI TGATCA 1 cut(s) 388
Fnu4HI GCNGC 1 cut(s) 279
Fsp4HI GCNGC 1 cut(s) 279
GluI GCNGC 1 cut(s) 279
HaeIII GGCC 1 cut(s) 258
Hin1II CATG 3 cut(s) 38, 248, 397
HinfI GANTC 2 cut(s) 66, 230
Hpy188I TCNGA 2 cut(s) 65, 338
HpyAV CCTTC 1 cut(s) 176
HpyCH4III ACNGT 1 cut(s) 254
HpyCH4V TGCA 1 cut(s) 199
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 278, 287
HpyF3I CTNAG 2 cut(s) 44, 294
Hsp92II CATG 3 cut(s) 38, 248, 397
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 2 cut(s) 37, 388
LmnI GCTCC 1 cut(s) 21
LpnPI CCDG 2 cut(s) 308, 352
Lsp1109I GCAGC 1 cut(s) 265
MalI GATC 2 cut(s) 39, 390
MboI GATC 2 cut(s) 37, 388
MboII GAAGA 4 cut(s) 176, 311, 386, 389
MhlI GDGCHC 1 cut(s) 26
MluCI AATT 3 cut(s) 113, 332, 406
MlyI GAGTC 2 cut(s) 60, 224
MmeI TCCRAC 1 cut(s) 43
MnlI CCTC 8 cut(s) 85, 97, 100, 151, 169, 172, 269, 327
MslI CAYNNNNRTG 1 cut(s) 398
MwoI GCNNNNNNNGC 3 cut(s) 30, 278, 287
NdeII GATC 2 cut(s) 37, 388
NlaIII CATG 3 cut(s) 38, 248, 397
NspI RCATGY 1 cut(s) 248
PaeI GCATGC 1 cut(s) 248
PkrI GCNGC 1 cut(s) 280
PleI GAGTC 2 cut(s) 60, 224
PpsI GAGTC 2 cut(s) 60, 224
RsaI GTAC 1 cut(s) 285
RsaNI GTAC 1 cut(s) 284
RseI CAYNNNNRTG 1 cut(s) 398
SatI GCNGC 1 cut(s) 279
Sau3AI GATC 2 cut(s) 37, 388
SchI GAGTC 2 cut(s) 60, 224
SduI GDGCHC 1 cut(s) 26
SetI ASST 1 cut(s) 371
SmiMI CAYNNNNRTG 1 cut(s) 398
SphI GCATGC 1 cut(s) 248
Sse9I AATT 3 cut(s) 113, 332, 406
TaaI ACNGT 1 cut(s) 254
TaqI TCGA 2 cut(s) 174, 261
TasI AATT 3 cut(s) 113, 332, 406
TscAI CASTG 2 cut(s) 259, 298
TseI GCWGC 1 cut(s) 278
TspRI CASTG 2 cut(s) 259, 298
XceI RCATGY 1 cut(s) 248
XcmI CCANNNNNNNNNTGG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.