Rroxscaffold_159G00432720

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000159
OG0000342
Physical Location & Seq
Reverse (-)
336949 .. 338726
1778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_159G00432720.1

Sequence Viewer

Length: 531 bp
ATGAATACTTTGAGCAATTTAAAAAAAGACTTCACAGTGTTTCTAAGGTCTCGTGCAGAAGAGTTGGTCCCTCAGGGTAGAATGGTCCTCACAATCAGGGGGAGAATAAATAGTCATGAACCAGCTTTATTTGAAATTCTTGGATTGAAAATCAATGATATGGTTTTAGAGGGTTTGATTGAAAAGAAAAACCTAGACTACTTCAATATTCCATACTATGAACCTACAATGGAGGAACTGATGGAGCTGATCGAGACCGAAGGATCCTTTAGGTTGCCGGACCATCAAGTGTTTAAGCGTGATTGGGACTCTTCTATAAAGGAAGCTGATAGTAGTCTCGGTAAGAAAGCAAGGGCGGGTAAACTTTCCACTCACATTAGGTCTGTGGTGGAGCCTCTTCTGATCAGTCACTTTGGAGAGGGACTCATGGAAGATTTGTTTCACAGGTTTGAAAAAGATGTTCTTGATCACATGGAAAAGGAGAAGTGCCAGTCTATAGATATAGTTCTATCGTTGACAAAGGAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

176

Amino Acids

20.44

Weight (kDa)

5.43

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 7 - 174 1.1e-45 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14060 AT2G14060 AT3G11480 AT3G21950 AT3G21950 AT5G04370 AT5G04370 AT5G04370 AT5G04380 AT5G04380 AT5G04380 AT5G38020 AT5G38020 AT5G66430
fragaria_vesca FvH4_2g09290 FvH4_2g09290 FvH4_2g09310 FvH4_3g03130
malus_domestica MD10G1071000.v1.1 MD10G1071200.v1.1 MD10G1071500.v1.1 MD10G1218400.v1.1
prunus_persica Prupe.8G092700_v2.0.a1 Prupe.8G092800_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G093000_v2.0.a1 Prupe.8G093100_v2.0.a1 Prupe.8G093200_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093600_v2.0.a1
pyrus_communis pycom10g05660 pycom10g05680 pycom10g18420
rosa_chinensis RchiOBHm_Chr1g0324421 RchiOBHm_Chr1g0324461 RchiOBHm_Chr6g0265311 RchiOBHm_Chr6g0265321 RchiOBHm_Chr6g0265361 RchiOBHm_Chr6g0265391 RchiOBHm_Chr6g0265411
rosa_laevigata RLG00000014186 RLG00000014191 RLG00000014195 RLG00000014197 RLG00000025381 RLG00000030199
rosa_multiflora Rmu_co8237181.1_g000001 Rmu_sc0001780.1_g000008 Rmu_sc0001803.1_g000020 Rmu_sc0002550.1_g000004 Rmu_sc0004082.1_g000012 Rmu_sc0008601.1_g000002 Rmu_sc0011954.1_g000001 Rmu_sc0013000.1_g000001 Rmu_sc0016482.1_g000001 Rmu_sc0018713.1_g000002 Rmu_ssc0000066.1_g000015
rosa_roxburghii Rroxscaffold_159G00432720 Rroxscaffold_159G00432790 Rroxscaffold_159G00432850 Rroxscaffold_175G00432320 Rroxscaffold_175G00432340 Rroxscaffold_175G00432350 Rroxscaffold_175G00432360 Rroxscaffold_2G00105600 Rroxscaffold_2G00105660 Rroxscaffold_4G00325530 Rroxscaffold_4G00325600 Rroxscaffold_4G00325670 Rroxscaffold_6G00424510 Rroxscaffold_7G00202680 Rroxscaffold_7G00202760 Rroxscaffold_7G00202800 Rroxscaffold_7G00202850
rosa_rugosa Rorug01G0045900 Rorug01G0046000 Rorug02G0355000 Rorug02G0355300 Rorug03G0004200 Rorug06G0016700 Rorug06G0016700 Rorug06G0016800 Rorug06G0017100
rosa_samantha Rh1AG061600 Rh1AG061700 Rh1BG052400 Rh1CG063500 Rh1CG063900 Rh1CG064100 Rh2BG415800 Rh3DG067100 Rh6AG138200 Rh6AG138400 Rh6AG138800 Rh6BG137100 Rh6BG137200 Rh6BG137600 Rh6DG121600 Rh6DG121700 Rh6DG121900
rosa_wichuraiana Rw1G005230 Rw1G005470 Rw2G033110 Rw6G011920 Rw6G011940 Rw6G011950 Rw6G011970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 356
AclWI GGATC 2 cut(s) 258, 271
AcsI RAATTY 1 cut(s) 135
AgsI TTSAA 5 cut(s) 134, 148, 182, 205, 452
AluBI AGCT 3 cut(s) 125, 247, 326
AluI AGCT 3 cut(s) 125, 247, 326
Alw26I GTCTC 3 cut(s) 54, 248, 341
AlwI GGATC 2 cut(s) 258, 271
ApoI RAATTY 1 cut(s) 135
AspS9I GGNCC 3 cut(s) 67, 85, 280
AvaII GGWCC 3 cut(s) 67, 85, 280
AxyI CCTNAGG 1 cut(s) 72
BamHI GGATCC 1 cut(s) 263
BauI CACGAG 1 cut(s) 51
BccI CCATC 2 cut(s) 235, 291
BclI TGATCA 2 cut(s) 402, 466
BcoDI GTCTC 3 cut(s) 54, 248, 341
BfaI CTAG 1 cut(s) 194
BfmI CTRYAG 1 cut(s) 495
Bme18I GGWCC 3 cut(s) 67, 85, 280
BmgT120I GGNCC 3 cut(s) 67, 85, 280
BmiI GGNNCC 3 cut(s) 69, 265, 393
BplI GAGNNNNNCTC 2 cut(s) 408, 440
BsaI GGTCTC 2 cut(s) 54, 248
Bse1I ACTGG 1 cut(s) 490
Bse21I CCTNAGG 1 cut(s) 72
BseMII CTCAG 1 cut(s) 86
BseNI ACTGG 1 cut(s) 490
BsgI GTGCAG 1 cut(s) 75
BsiSI CCGG 1 cut(s) 278
BslFI GGGAC 3 cut(s) 53, 320, 435
BsmAI GTCTC 3 cut(s) 54, 248, 341
BsmFI GGGAC 3 cut(s) 53, 320, 435
Bso31I GGTCTC 2 cut(s) 54, 248
Bsp143I GATC 4 cut(s) 249, 263, 402, 466
BspACI CCGC 1 cut(s) 356
BspCNI CTCAG 1 cut(s) 85
BspHI TCATGA 1 cut(s) 115
BspLI GGNNCC 3 cut(s) 69, 265, 393
BspPI GGATC 2 cut(s) 258, 271
BspTNI GGTCTC 2 cut(s) 54, 248
BsrI ACTGG 1 cut(s) 490
BssMI GATC 4 cut(s) 249, 263, 402, 466
BssSI CACGAG 1 cut(s) 51
Bst2BI CACGAG 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 3 cut(s) 54, 316, 402
BstDEI CTNAG 2 cut(s) 44, 72
BstKTI GATC 4 cut(s) 252, 266, 405, 469
BstMAI GTCTC 3 cut(s) 54, 248, 341
BstMBI GATC 4 cut(s) 249, 263, 402, 466
BstSFI CTRYAG 1 cut(s) 495
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
Bsu36I CCTNAGG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 42
CciI TCATGA 1 cut(s) 115
Cfr13I GGNCC 3 cut(s) 67, 85, 280
CviAII CATG 3 cut(s) 116, 427, 472
CviJI RGCY 4 cut(s) 125, 247, 326, 394
CviKI_1 RGCY 4 cut(s) 125, 247, 326, 394
DdeI CTNAG 2 cut(s) 44, 72
DpnI GATC 4 cut(s) 251, 265, 404, 468
DpnII GATC 4 cut(s) 249, 263, 402, 466
DraI TTTAAA 1 cut(s) 21
Eam1104I CTCTTC 3 cut(s) 54, 316, 402
EarI CTCTTC 3 cut(s) 54, 316, 402
Eco31I GGTCTC 2 cut(s) 54, 248
Eco47I GGWCC 3 cut(s) 67, 85, 280
Eco81I CCTNAGG 1 cut(s) 72
FaeI CATG 3 cut(s) 119, 430, 475
FaiI YATR 9 cut(s) 117, 161, 214, 219, 317, 428, 473, 497, 503
FalI AAGNNNNNCTT 2 cut(s) 447, 479
FaqI GGGAC 3 cut(s) 53, 320, 435
FatI CATG 3 cut(s) 115, 426, 471
FauI CCCGC 1 cut(s) 349
FbaI TGATCA 2 cut(s) 402, 466
FspBI CTAG 1 cut(s) 194
HapII CCGG 1 cut(s) 278
Hin1II CATG 3 cut(s) 119, 430, 475
HincII GTYRAC 1 cut(s) 516
HindII GTYRAC 1 cut(s) 516
HinfI GANTC 2 cut(s) 308, 423
HpaII CCGG 1 cut(s) 278
Hpy166II GTNNAC 2 cut(s) 362, 516
Hpy188I TCNGA 1 cut(s) 402
Hpy188III TCNNGA 3 cut(s) 116, 253, 464
Hpy8I GTNNAC 2 cut(s) 362, 516
HpyAV CCTTC 1 cut(s) 254
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4V TGCA 1 cut(s) 56
HpyF3I CTNAG 2 cut(s) 44, 72
Hsp92II CATG 3 cut(s) 119, 430, 475
Ksp22I TGATCA 2 cut(s) 402, 466
Kzo9I GATC 4 cut(s) 249, 263, 402, 466
LmnI GCTCC 2 cut(s) 244, 391
LpnPI CCDG 6 cut(s) 59, 82, 135, 291, 430, 503
MaeI CTAG 1 cut(s) 194
MaeIII GTNAC 1 cut(s) 407
MalI GATC 4 cut(s) 251, 265, 404, 468
MboI GATC 4 cut(s) 249, 263, 402, 466
MboII GAAGA 4 cut(s) 71, 303, 389, 443
MflI RGATCY 1 cut(s) 263
MluCI AATT 2 cut(s) 16, 135
MlyI GAGTC 2 cut(s) 302, 417
MnlI CCTC 6 cut(s) 81, 98, 163, 226, 405, 412
MseI TTAA 2 cut(s) 20, 294
MspI CCGG 1 cut(s) 278
NdeII GATC 4 cut(s) 249, 263, 402, 466
NlaIII CATG 3 cut(s) 119, 430, 475
NlaIV GGNNCC 3 cut(s) 69, 265, 393
NmuCI GTSAC 1 cut(s) 407
PagI TCATGA 1 cut(s) 115
PleI GAGTC 2 cut(s) 302, 417
PpsI GAGTC 2 cut(s) 302, 417
PspN4I GGNNCC 3 cut(s) 69, 265, 393
PspPI GGNCC 3 cut(s) 67, 85, 280
PsuI RGATCY 1 cut(s) 263
SaqAI TTAA 2 cut(s) 20, 294
Sau3AI GATC 4 cut(s) 249, 263, 402, 466
Sau96I GGNCC 3 cut(s) 67, 85, 280
SchI GAGTC 2 cut(s) 302, 417
SetI ASST 9 cut(s) 50, 127, 195, 226, 249, 275, 328, 383, 449
SfcI CTRYAG 1 cut(s) 495
SinI GGWCC 3 cut(s) 67, 85, 280
Sse9I AATT 2 cut(s) 16, 135
SsiI CCGC 1 cut(s) 356
SspI AATATT 1 cut(s) 208
SspMI CTAG 1 cut(s) 194
TaaI ACNGT 1 cut(s) 37
TaqI TCGA 1 cut(s) 252
TaqII GACCGA 1 cut(s) 272
TasI AATT 2 cut(s) 16, 135
Tru1I TTAA 2 cut(s) 20, 294
Tru9I TTAA 2 cut(s) 20, 294
TscAI CASTG 1 cut(s) 42
TseFI GTSAC 1 cut(s) 407
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 3 cut(s) 17, 132, 234
TspRI CASTG 1 cut(s) 42
VpaK11BI GGWCC 3 cut(s) 67, 85, 280
XapI RAATTY 1 cut(s) 135
XspI CTAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.