Rw2G033110

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000342
Physical Location & Seq
Reverse (-)
54269401 .. 54288788
19388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033110.1

Sequence Viewer

Length: 855 bp
ATGGAGGTAGAGCAAGTCCTTCACATGAATGGTGGAGATGGAAAAAGAACAGTCATTTCAAGGGTGAAGCCAATAGTAGATGCAAGCATAGAGAAGCTGTGTTGCACCCACCTCCCTACAGAGTGCTTGAAAATATCATTGCCAGACTTCTATAAGAAACTTGATGAAAGGAAGTGCATCGAACCATGTCTCATTGCAGGGATCAATTCTCTCCACTTTGGGCACTCTAGTTATTCTCTTATGTGGATCTCTAAGGTTCCAAAAGGTTTGGTAACGAAAGGAGGAGAAGGACTGAACAAGGGGAACATATACAGAGCCAAGACAAGCTCACATGCTGTGTTTAACGAATACTTTGAGCAATTCAAAAGGGACTGCACTGTCTTTCTGAGGTCTCGAGCACAAGAGCTGGTCTCGGGAGGTAGTATGGTCCTCACAACCATGGGCAGCATACGGAGCAATGATCCCCTCAGCATTTGGGAATTTGTCGGACTAAAACTCCATGACATGGTTTTAGATGGTTTGATTGAAGAGAAAAAATTGGACACATTTAATATGCCATACTATGCGTCAACAGCGGATGAGGTGAAAGAGGTGATTGAGGCTGAAGGTTCTTTTATTTTACAAAACCTCGAAACTTTTAGAAATGACTGGGACTGTTACATAAAACAAGTTAACTGTGGCTTTGACAAGAAAAGCTGTGGGAGAGCCTATTCTCTGGCCAGCGGATTCAGAGAAAAAGCCATGGATGATTTGTTTTGCAGGTTTGAAGAAGATGTTCTTGATCACATGGAGAAGGATAACTGCCAGTTCATTGACCTGGTTATCTCGTTTGTTGGAAATTTTGGAGATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

284

Amino Acids

32.13

Weight (kDa)

5.47

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 69 - 276 1.7e-56 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14060 AT2G14060 AT3G11480 AT3G21950 AT3G21950 AT5G04370 AT5G04370 AT5G04370 AT5G04380 AT5G04380 AT5G04380 AT5G38020 AT5G38020 AT5G66430
fragaria_vesca FvH4_2g09290 FvH4_2g09290 FvH4_2g09310 FvH4_3g03130
malus_domestica MD10G1071000.v1.1 MD10G1071200.v1.1 MD10G1071500.v1.1 MD10G1218400.v1.1
prunus_persica Prupe.8G092700_v2.0.a1 Prupe.8G092800_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G093000_v2.0.a1 Prupe.8G093100_v2.0.a1 Prupe.8G093200_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093600_v2.0.a1
pyrus_communis pycom10g05660 pycom10g05680 pycom10g18420
rosa_chinensis RchiOBHm_Chr1g0324421 RchiOBHm_Chr1g0324461 RchiOBHm_Chr6g0265311 RchiOBHm_Chr6g0265321 RchiOBHm_Chr6g0265361 RchiOBHm_Chr6g0265391 RchiOBHm_Chr6g0265411
rosa_laevigata RLG00000014186 RLG00000014191 RLG00000014195 RLG00000014197 RLG00000025381 RLG00000030199
rosa_multiflora Rmu_co8237181.1_g000001 Rmu_sc0001780.1_g000008 Rmu_sc0001803.1_g000020 Rmu_sc0002550.1_g000004 Rmu_sc0004082.1_g000012 Rmu_sc0008601.1_g000002 Rmu_sc0011954.1_g000001 Rmu_sc0013000.1_g000001 Rmu_sc0016482.1_g000001 Rmu_sc0018713.1_g000002 Rmu_ssc0000066.1_g000015
rosa_roxburghii Rroxscaffold_159G00432720 Rroxscaffold_159G00432790 Rroxscaffold_159G00432850 Rroxscaffold_175G00432320 Rroxscaffold_175G00432340 Rroxscaffold_175G00432350 Rroxscaffold_175G00432360 Rroxscaffold_2G00105600 Rroxscaffold_2G00105660 Rroxscaffold_4G00325530 Rroxscaffold_4G00325600 Rroxscaffold_4G00325670 Rroxscaffold_6G00424510 Rroxscaffold_7G00202680 Rroxscaffold_7G00202760 Rroxscaffold_7G00202800 Rroxscaffold_7G00202850
rosa_rugosa Rorug01G0045900 Rorug01G0046000 Rorug02G0355000 Rorug02G0355300 Rorug03G0004200 Rorug06G0016700 Rorug06G0016700 Rorug06G0016800 Rorug06G0017100
rosa_samantha Rh1AG061600 Rh1AG061700 Rh1BG052400 Rh1CG063500 Rh1CG063900 Rh1CG064100 Rh2BG415800 Rh3DG067100 Rh6AG138200 Rh6AG138400 Rh6AG138800 Rh6BG137100 Rh6BG137200 Rh6BG137600 Rh6DG121600 Rh6DG121700 Rh6DG121900
rosa_wichuraiana Rw1G005230 Rw1G005470 Rw2G033110 Rw6G011920 Rw6G011940 Rw6G011950 Rw6G011970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 377
Acc36I ACCTGC 1 cut(s) 750
AccB7I CCANNNNNTGG 1 cut(s) 505
AciI CCGC 2 cut(s) 575, 723
AclWI GGATC 3 cut(s) 209, 254, 455
AcoI YGGCCR 1 cut(s) 717
AcsI RAATTY 2 cut(s) 479, 838
AcuI CTGAAG 1 cut(s) 624
AfiI CCNNNNNNNGG 1 cut(s) 505
AgsI TTSAA 5 cut(s) 60, 130, 364, 527, 767
AjnI CCWGG 1 cut(s) 816
AluBI AGCT 4 cut(s) 97, 327, 406, 696
AluI AGCT 4 cut(s) 97, 327, 406, 696
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 3 cut(s) 194, 396, 415
AlwI GGATC 3 cut(s) 209, 254, 455
Ama87I CYCGRG 2 cut(s) 393, 412
AoxI GGCC 1 cut(s) 717
ApeKI GCWGC 1 cut(s) 444
ApoI RAATTY 2 cut(s) 479, 838
Asp700I GAANNNNTTC 1 cut(s) 774
AspS9I GGNCC 1 cut(s) 427
AsuHPI GGTGA 3 cut(s) 76, 595, 604
AvaI CYCGRG 2 cut(s) 393, 412
AvaII GGWCC 1 cut(s) 427
BaeGI GKGCMC 1 cut(s) 225
BalI TGGCCA 1 cut(s) 719
Bbv12I GWGCWC 1 cut(s) 400
BbvCI CCTCAGC 1 cut(s) 467
BbvI GCAGC 1 cut(s) 456
BccI CCATC 3 cut(s) 32, 509, 842
BciT130I CCWGG 1 cut(s) 818
BclI TGATCA 1 cut(s) 781
BcoDI GTCTC 3 cut(s) 194, 396, 415
BfaI CTAG 1 cut(s) 228
BfmI CTRYAG 1 cut(s) 117
BfuAI ACCTGC 1 cut(s) 750
BisI GCNGC 1 cut(s) 445
BlsI GCNGC 1 cut(s) 446
Bme1390I CCNGG 1 cut(s) 818
Bme18I GGWCC 1 cut(s) 427
BmeT110I CYCGRG 2 cut(s) 393, 412
BmgT120I GGNCC 1 cut(s) 427
BmiI GGNNCC 1 cut(s) 258
BmrFI CCNGG 1 cut(s) 818
BmrI ACTGGG 1 cut(s) 658
BmsI GCATC 2 cut(s) 70, 186
BmuI ACTGGG 1 cut(s) 658
BplI GAGNNNNNCTC 2 cut(s) 395, 427
Bpu10I CCTNAGC 1 cut(s) 467
BsaI GGTCTC 2 cut(s) 396, 415
BsaJI CCNNGG 2 cut(s) 438, 741
BsaXI ACNNNNNCTCC 2 cut(s) 480, 510
Bsc4I CCNNNNNNNGG 1 cut(s) 505
Bse1I ACTGG 2 cut(s) 653, 805
Bse3DI GCAATG 3 cut(s) 137, 192, 463
BseBI CCWGG 1 cut(s) 818
BseDI CCNNGG 2 cut(s) 438, 741
BseGI GGATG 2 cut(s) 583, 751
BseLI CCNNNNNNNGG 1 cut(s) 505
BseMI GCAATG 3 cut(s) 137, 192, 463
BseMII CTCAG 2 cut(s) 377, 481
BseNI ACTGG 2 cut(s) 653, 805
BseRI GAGGAG 1 cut(s) 297
BseSI GKGCMC 1 cut(s) 225
BseXI GCAGC 1 cut(s) 456
BsgI GTGCAG 1 cut(s) 358
BshFI GGCC 1 cut(s) 719
BsiHKAI GWGCWC 1 cut(s) 400
BsiHKCI CYCGRG 2 cut(s) 393, 412
BslFI GGGAC 2 cut(s) 383, 665
BslI CCNNNNNNNGG 1 cut(s) 505
BsmAI GTCTC 3 cut(s) 194, 396, 415
BsmFI GGGAC 2 cut(s) 383, 665
BsnI GGCC 1 cut(s) 719
Bso31I GGTCTC 2 cut(s) 396, 415
BsoBI CYCGRG 2 cut(s) 393, 412
Bsp1286I GDGCHC 2 cut(s) 225, 400
Bsp143I GATC 4 cut(s) 201, 246, 460, 781
Bsp19I CCATGG 2 cut(s) 438, 741
BspACI CCGC 2 cut(s) 575, 723
BspANI GGCC 1 cut(s) 719
BspCNI CTCAG 2 cut(s) 378, 480
BspLI GGNNCC 1 cut(s) 258
BspMI ACCTGC 1 cut(s) 750
BspPI GGATC 3 cut(s) 209, 254, 455
BspTNI GGTCTC 2 cut(s) 396, 415
BsrDI GCAATG 3 cut(s) 137, 192, 463
BsrI ACTGG 2 cut(s) 653, 805
BssECI CCNNGG 2 cut(s) 438, 741
BssMI GATC 4 cut(s) 201, 246, 460, 781
BssT1I CCWWGG 2 cut(s) 438, 741
Bst2UI CCWGG 1 cut(s) 818
Bst4CI ACNGT 4 cut(s) 52, 379, 656, 677
Bst6I CTCTTC 1 cut(s) 522
BstC8I GCNNGC 2 cut(s) 85, 721
BstDEI CTNAG 3 cut(s) 252, 386, 467
BstDSI CCRYGG 2 cut(s) 438, 741
BstF5I GGATG 2 cut(s) 583, 751
BstKTI GATC 4 cut(s) 204, 249, 463, 784
BstMAI GTCTC 3 cut(s) 194, 396, 415
BstMBI GATC 4 cut(s) 201, 246, 460, 781
BstMWI GCNNNNNNNGC 2 cut(s) 453, 572
BstNI CCWGG 1 cut(s) 818
BstNSI RCATGY 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 816
BstSFI CTRYAG 1 cut(s) 117
BstSLI GKGCMC 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 456
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
BsuRI GGCC 1 cut(s) 719
BtgI CCRYGG 2 cut(s) 438, 741
BtsCI GGATG 2 cut(s) 583, 751
BtsIMutI CAGTG 1 cut(s) 375
BveI ACCTGC 1 cut(s) 750
Cac8I GCNNGC 2 cut(s) 85, 721
Cfr13I GGNCC 1 cut(s) 427
CseI GACGC 1 cut(s) 555
CsiI ACCWGGT 1 cut(s) 816
CviAII CATG 8 cut(s) 25, 186, 332, 439, 500, 505, 742, 787
DdeI CTNAG 3 cut(s) 252, 386, 467
DpnI GATC 4 cut(s) 203, 248, 462, 783
DpnII GATC 4 cut(s) 201, 246, 460, 781
DrdI GACNNNNNNGTC 1 cut(s) 377
DseDI GACNNNNNNGTC 1 cut(s) 377
EaeI YGGCCR 1 cut(s) 717
Eam1104I CTCTTC 1 cut(s) 522
EarI CTCTTC 1 cut(s) 522
Eco130I CCWWGG 2 cut(s) 438, 741
Eco31I GGTCTC 2 cut(s) 396, 415
Eco47I GGWCC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 624
Eco88I CYCGRG 2 cut(s) 393, 412
EcoRII CCWGG 1 cut(s) 816
EcoT14I CCWWGG 2 cut(s) 438, 741
ErhI CCWWGG 2 cut(s) 438, 741
FaeI CATG 8 cut(s) 28, 189, 335, 442, 503, 508, 745, 790
FalI AAGNNNNNCTT 2 cut(s) 762, 794
FaqI GGGAC 2 cut(s) 383, 665
FatI CATG 8 cut(s) 24, 185, 331, 438, 499, 504, 741, 786
FbaI TGATCA 1 cut(s) 781
Fnu4HI GCNGC 1 cut(s) 445
FokI GGATG 2 cut(s) 590, 758
Fsp4HI GCNGC 1 cut(s) 445
FspBI CTAG 1 cut(s) 228
GluI GCNGC 1 cut(s) 445
HaeIII GGCC 1 cut(s) 719
HgaI GACGC 1 cut(s) 555
Hin1II CATG 8 cut(s) 28, 189, 335, 442, 503, 508, 745, 790
HincII GTYRAC 2 cut(s) 570, 673
HindII GTYRAC 2 cut(s) 570, 673
HinfI GANTC 1 cut(s) 726
HpaI GTTAAC 1 cut(s) 673
HphI GGTGA 3 cut(s) 76, 595, 604
Hpy166II GTNNAC 2 cut(s) 570, 673
Hpy188I TCNGA 3 cut(s) 387, 488, 731
Hpy188III TCNNGA 3 cut(s) 393, 414, 779
Hpy8I GTNNAC 2 cut(s) 570, 673
HpyAV CCTTC 4 cut(s) 29, 281, 599, 787
HpyCH4III ACNGT 4 cut(s) 52, 379, 656, 677
HpyCH4V TGCA 6 cut(s) 83, 105, 177, 197, 375, 759
HpyF10VI GCNNNNNNNGC 2 cut(s) 453, 572
HpyF3I CTNAG 3 cut(s) 252, 386, 467
Hsp92II CATG 8 cut(s) 28, 189, 335, 442, 503, 508, 745, 790
Ksp22I TGATCA 1 cut(s) 781
KspAI GTTAAC 1 cut(s) 673
Kzo9I GATC 4 cut(s) 201, 246, 460, 781
LmnI GCTCC 1 cut(s) 453
Lsp1109I GCAGC 1 cut(s) 456
LweI GCATC 2 cut(s) 70, 186
MabI ACCWGGT 1 cut(s) 816
MaeI CTAG 1 cut(s) 228
MaeIII GTNAC 2 cut(s) 271, 656
MalI GATC 4 cut(s) 203, 248, 462, 783
MboI GATC 4 cut(s) 201, 246, 460, 781
MboII GAAGA 3 cut(s) 539, 779, 782
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 2 cut(s) 225, 400
MlsI TGGCCA 1 cut(s) 719
MluCI AATT 5 cut(s) 205, 359, 479, 536, 838
MluNI TGGCCA 1 cut(s) 719
MmeI TCCRAC 2 cut(s) 466, 814
Mox20I TGGCCA 1 cut(s) 719
MroXI GAANNNNTTC 1 cut(s) 774
MscI TGGCCA 1 cut(s) 719
MseI TTAA 3 cut(s) 342, 549, 672
MslI CAYNNNNRTG 2 cut(s) 27, 437
Msp20I TGGCCA 1 cut(s) 719
MspA1I CMGCKG 2 cut(s) 575, 723
MspR9I CCNGG 1 cut(s) 818
MvaI CCWGG 1 cut(s) 818
MwoI GCNNNNNNNGC 2 cut(s) 453, 572
NcoI CCATGG 2 cut(s) 438, 741
NdeII GATC 4 cut(s) 201, 246, 460, 781
NlaIII CATG 8 cut(s) 28, 189, 335, 442, 503, 508, 745, 790
NlaIV GGNNCC 1 cut(s) 258
NspI RCATGY 1 cut(s) 335
PaeR7I CTCGAG 1 cut(s) 393
PdmI GAANNNNTTC 1 cut(s) 774
PfeI GAWTC 1 cut(s) 726
PflMI CCANNNNNTGG 1 cut(s) 505
PkrI GCNGC 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 816
PspGI CCWGG 1 cut(s) 816
PspN4I GGNNCC 1 cut(s) 258
PspPI GGNCC 1 cut(s) 427
PsuI RGATCY 1 cut(s) 246
RseI CAYNNNNRTG 2 cut(s) 27, 437
SaqAI TTAA 3 cut(s) 342, 549, 672
SatI GCNGC 1 cut(s) 445
Sau3AI GATC 4 cut(s) 201, 246, 460, 781
Sau96I GGNCC 1 cut(s) 427
ScrFI CCNGG 1 cut(s) 818
SduI GDGCHC 2 cut(s) 225, 400
SexAI ACCWGGT 1 cut(s) 816
SfaNI GCATC 2 cut(s) 70, 186
SfcI CTRYAG 1 cut(s) 117
Sfr274I CTCGAG 1 cut(s) 393
SinI GGWCC 1 cut(s) 427
SlaI CTCGAG 1 cut(s) 393
SmiMI CAYNNNNRTG 2 cut(s) 27, 437
SmlI CTYRAG 1 cut(s) 393
SmoI CTYRAG 1 cut(s) 393
Sse9I AATT 5 cut(s) 205, 359, 479, 536, 838
SsiI CCGC 2 cut(s) 575, 723
SspMI CTAG 1 cut(s) 228
StyD4I CCNGG 1 cut(s) 816
StyI CCWWGG 2 cut(s) 438, 741
TaaI ACNGT 4 cut(s) 52, 379, 656, 677
TaqI TCGA 3 cut(s) 180, 394, 630
TasI AATT 5 cut(s) 205, 359, 479, 536, 838
TfiI GAWTC 1 cut(s) 726
Tru1I TTAA 3 cut(s) 342, 549, 672
Tru9I TTAA 3 cut(s) 342, 549, 672
TscAI CASTG 1 cut(s) 382
TseI GCWGC 1 cut(s) 444
TspDTI ATGAA 3 cut(s) 41, 180, 799
TspGWI ACGGA 1 cut(s) 466
TspRI CASTG 1 cut(s) 382
Van91I CCANNNNNTGG 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 427
XapI RAATTY 2 cut(s) 479, 838
XceI RCATGY 1 cut(s) 335
XhoI CTCGAG 1 cut(s) 393
XmnI GAANNNNTTC 1 cut(s) 774
XspI CTAG 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.