pycom10g18420

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000342
Physical Location & Seq
Forward (+)
21704085 .. 21705472
1388 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g18420.6

Sequence Viewer

Length: 1071 bp
ATGCTTAAGTTAAATAAAACCCACAAGGCAAATGGCTTTTGTTTCAAAGTTGACTGGATTTGTAATTCCGTAGACTGGAGCACCATATTCAAAGTAATTCCGAAACCCATATATAAATATTTGAGAATACAAACTAAAATGGAGGTAGATCAGCAAGTTCTTCACATGAATGGAGGAGTTGGGAAAACAAGCTATGCAACCAACTCTCTACTTCAAAGAGCTGCGATTTCAATGGTGAAGCCCACAGTTAAGGCAAGCATAGAGCAGCTTTCTAGGAAACTCTTCTCTGACTGTTTGAAAATAGCAGACTTGGGATGCTCTTCCGGACCCAACACCCTTCTGGTGGTATCAGATATCATACACAACATCCATGCCACTTTCCGAAAACTGAACCGTCCATCTCCTTCACTCCAAGCCTTCTTGAATGATCTTCCCGGAAACGATTTCAACACTCTGTTCAGGTCATTGCCAGGGTTCTATAAGAAACTTGAGGAAGAACAGTTAGGGCCATGTTTCATTACTGGAATGCCTGGTTCTTTCTATGGCAGGCTCTTTCCAAACAATTCTCTCCACTTTATTCACTCTAGTTATGCCCTTCTGTGGATCTCTGAGGCTCCAAAAGGTTTGATAACCAAGGAAGGAGAGGGACTAAACAAGAAGAACATATACATAGCGAAAACAAGCCCACCTGCTGTGTATAAGCAGTACTTGGAGCAATTCAAAAAGGACTTCAGAGTGTTTTTGAGATCACGAGCAGAAGAGCTGGTTCCCGGAGGAAGTATGGTCCTCACAACATTGGGGAGCAAAAAGAGCCATCGATCCACTTACAAAAATTGGACACATTCAATATGCCATTGTATTTTCCTACAACAAAGGAGGTTGAAGATGTCATCGAGGCGGAAGGATTTTTTACTTTGCAAACACGCTGACAGTGGGCTGTGGGAGAGCCTATTCTGGCAACCCAATTCGGAGAAGTGGCTATGGACGATTTGTTTCGCAGGTTTGAAGAAGATGTCCTTGATCACATGGAAATGGAGAATTGCCAGTTCATTGACCTGGTTATCTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

357

Amino Acids

40.86

Weight (kDa)

9.84

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14060 AT2G14060 AT3G11480 AT3G21950 AT3G21950 AT5G04370 AT5G04370 AT5G04370 AT5G04380 AT5G04380 AT5G04380 AT5G38020 AT5G38020 AT5G66430
fragaria_vesca FvH4_2g09290 FvH4_2g09290 FvH4_2g09310 FvH4_3g03130
malus_domestica MD10G1071000.v1.1 MD10G1071200.v1.1 MD10G1071500.v1.1 MD10G1218400.v1.1
prunus_persica Prupe.8G092700_v2.0.a1 Prupe.8G092800_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G093000_v2.0.a1 Prupe.8G093100_v2.0.a1 Prupe.8G093200_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093600_v2.0.a1
pyrus_communis pycom10g05660 pycom10g05680 pycom10g18420
rosa_chinensis RchiOBHm_Chr1g0324421 RchiOBHm_Chr1g0324461 RchiOBHm_Chr6g0265311 RchiOBHm_Chr6g0265321 RchiOBHm_Chr6g0265361 RchiOBHm_Chr6g0265391 RchiOBHm_Chr6g0265411
rosa_laevigata RLG00000014186 RLG00000014191 RLG00000014195 RLG00000014197 RLG00000025381 RLG00000030199
rosa_multiflora Rmu_co8237181.1_g000001 Rmu_sc0001780.1_g000008 Rmu_sc0001803.1_g000020 Rmu_sc0002550.1_g000004 Rmu_sc0004082.1_g000012 Rmu_sc0008601.1_g000002 Rmu_sc0011954.1_g000001 Rmu_sc0013000.1_g000001 Rmu_sc0016482.1_g000001 Rmu_sc0018713.1_g000002 Rmu_ssc0000066.1_g000015
rosa_roxburghii Rroxscaffold_159G00432720 Rroxscaffold_159G00432790 Rroxscaffold_159G00432850 Rroxscaffold_175G00432320 Rroxscaffold_175G00432340 Rroxscaffold_175G00432350 Rroxscaffold_175G00432360 Rroxscaffold_2G00105600 Rroxscaffold_2G00105660 Rroxscaffold_4G00325530 Rroxscaffold_4G00325600 Rroxscaffold_4G00325670 Rroxscaffold_6G00424510 Rroxscaffold_7G00202680 Rroxscaffold_7G00202760 Rroxscaffold_7G00202800 Rroxscaffold_7G00202850
rosa_rugosa Rorug01G0045900 Rorug01G0046000 Rorug02G0355000 Rorug02G0355300 Rorug03G0004200 Rorug06G0016700 Rorug06G0016700 Rorug06G0016800 Rorug06G0017100
rosa_samantha Rh1AG061600 Rh1AG061700 Rh1BG052400 Rh1CG063500 Rh1CG063900 Rh1CG064100 Rh2BG415800 Rh3DG067100 Rh6AG138200 Rh6AG138400 Rh6AG138800 Rh6BG137100 Rh6BG137200 Rh6BG137600 Rh6DG121600 Rh6DG121700 Rh6DG121900
rosa_wichuraiana Rw1G005230 Rw1G005470 Rw2G033110 Rw6G011920 Rw6G011940 Rw6G011950 Rw6G011970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 697
Acc36I ACCTGC 2 cut(s) 697, 989
AccI GTMKAC 1 cut(s) 72
AccIII TCCGGA 1 cut(s) 323
AciI CCGC 1 cut(s) 898
AclWI GGATC 2 cut(s) 611, 813
AcuI CTGAAG 1 cut(s) 715
AfaI GTAC 1 cut(s) 707
AfiI CCNNNNNNNGG 3 cut(s) 75, 343, 600
AflII CTTAAG 1 cut(s) 5
AjnI CCWGG 3 cut(s) 469, 529, 1055
AluBI AGCT 4 cut(s) 192, 221, 268, 763
AluI AGCT 4 cut(s) 192, 221, 268, 763
Alw21I GWGCWC 1 cut(s) 83
AlwI GGATC 2 cut(s) 611, 813
Aor13HI TCCGGA 1 cut(s) 323
AoxI GGCC 1 cut(s) 506
ApeKI GCWGC 2 cut(s) 221, 265
Asp700I GAANNNNTTC 1 cut(s) 281
AspS9I GGNCC 3 cut(s) 326, 506, 784
AsuC2I CCSGG 2 cut(s) 435, 771
AsuHPI GGTGA 1 cut(s) 247
AvaII GGWCC 2 cut(s) 326, 784
BarI GAAGNNNNNNTAC 2 cut(s) 650, 682
BauI CACGAG 1 cut(s) 750
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 2 cut(s) 208, 277
BccI CCATC 2 cut(s) 406, 822
BciT130I CCWGG 3 cut(s) 471, 531, 1057
BclI TGATCA 1 cut(s) 1020
BcnI CCSGG 2 cut(s) 435, 771
BfaI CTAG 2 cut(s) 273, 585
BfrI CTTAAG 1 cut(s) 5
BfuAI ACCTGC 2 cut(s) 697, 989
BisI GCNGC 2 cut(s) 222, 266
BlsI GCNGC 2 cut(s) 223, 267
BmcAI AGTACT 1 cut(s) 707
Bme1390I CCNGG 5 cut(s) 435, 471, 531, 771, 1057
Bme18I GGWCC 2 cut(s) 326, 784
BmgT120I GGNCC 3 cut(s) 326, 506, 784
BmiI GGNNCC 3 cut(s) 328, 615, 768
BmrFI CCNGG 5 cut(s) 435, 471, 531, 771, 1057
BmsI GCATC 1 cut(s) 305
BpmI CTGGAG 1 cut(s) 97
BpuEI CTTGAG 1 cut(s) 509
BpuMI CCSGG 2 cut(s) 435, 771
Bsa29I ATCGAT 1 cut(s) 817
BsaJI CCNNGG 2 cut(s) 470, 633
BsaWI WCCGGW 1 cut(s) 323
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 343, 600
Bse1I ACTGG 4 cut(s) 59, 80, 526, 1044
Bse3DI GCAATG 1 cut(s) 464
BseAI TCCGGA 1 cut(s) 323
BseBI CCWGG 3 cut(s) 471, 531, 1057
BseCI ATCGAT 1 cut(s) 817
BseDI CCNNGG 2 cut(s) 470, 633
BseGI GGATG 2 cut(s) 320, 366
BseLI CCNNNNNNNGG 3 cut(s) 75, 343, 600
BseMI GCAATG 1 cut(s) 464
BseMII CTCAG 1 cut(s) 600
BseNI ACTGG 4 cut(s) 59, 80, 526, 1044
BseRI GAGGAG 1 cut(s) 189
BseXI GCAGC 2 cut(s) 208, 277
BshFI GGCC 1 cut(s) 508
BshVI ATCGAT 1 cut(s) 817
BsiHKAI GWGCWC 1 cut(s) 83
BsiSI CCGG 3 cut(s) 324, 435, 771
BslFI GGGAC 1 cut(s) 660
BslI CCNNNNNNNGG 3 cut(s) 75, 343, 600
BsmFI GGGAC 1 cut(s) 660
BsmI GAATGC 1 cut(s) 531
BsnI GGCC 1 cut(s) 508
Bsp1286I GDGCHC 1 cut(s) 83
Bsp13I TCCGGA 1 cut(s) 323
Bsp143I GATC 6 cut(s) 148, 427, 603, 746, 818, 1020
BspACI CCGC 1 cut(s) 898
BspANI GGCC 1 cut(s) 508
BspCNI CTCAG 1 cut(s) 601
BspDI ATCGAT 1 cut(s) 817
BspEI TCCGGA 1 cut(s) 323
BspLI GGNNCC 3 cut(s) 328, 615, 768
BspMI ACCTGC 2 cut(s) 697, 989
BspPI GGATC 2 cut(s) 611, 813
BspQI GCTCTTC 2 cut(s) 325, 753
BspTI CTTAAG 1 cut(s) 5
BsrDI GCAATG 1 cut(s) 464
BsrI ACTGG 4 cut(s) 59, 80, 526, 1044
BssECI CCNNGG 2 cut(s) 470, 633
BssMI GATC 6 cut(s) 148, 427, 603, 746, 818, 1020
BssSI CACGAG 1 cut(s) 750
BssT1I CCWWGG 1 cut(s) 633
Bst2BI CACGAG 1 cut(s) 750
Bst2UI CCWGG 3 cut(s) 471, 531, 1057
Bst4CI ACNGT 5 cut(s) 247, 293, 395, 501, 932
Bst6I CTCTTC 3 cut(s) 287, 325, 753
BstAFI CTTAAG 1 cut(s) 5
BstC8I GCNNGC 2 cut(s) 256, 548
BstDEI CTNAG 1 cut(s) 609
BstF5I GGATG 2 cut(s) 320, 366
BstKTI GATC 6 cut(s) 151, 430, 606, 749, 821, 1023
BstMBI GATC 6 cut(s) 148, 427, 603, 746, 818, 1020
BstMWI GCNNNNNNNGC 1 cut(s) 810
BstNI CCWGG 3 cut(s) 471, 531, 1057
BstSCI CCNGG 5 cut(s) 433, 469, 529, 769, 1055
BstV1I GCAGC 2 cut(s) 208, 277
BstX2I RGATCY 1 cut(s) 603
BstYI RGATCY 1 cut(s) 603
Bsu15I ATCGAT 1 cut(s) 817
BsuRI GGCC 1 cut(s) 508
BsuTUI ATCGAT 1 cut(s) 817
BtsCI GGATG 2 cut(s) 320, 366
BtsIMutI CAGTG 1 cut(s) 937
BveI ACCTGC 2 cut(s) 697, 989
Cac8I GCNNGC 2 cut(s) 256, 548
Cfr13I GGNCC 3 cut(s) 326, 506, 784
ClaI ATCGAT 1 cut(s) 817
CsiI ACCWGGT 1 cut(s) 1055
Csp6I GTAC 1 cut(s) 706
CviAII CATG 4 cut(s) 166, 371, 510, 1026
CviQI GTAC 1 cut(s) 706
DdeI CTNAG 1 cut(s) 609
DpnI GATC 6 cut(s) 150, 429, 605, 748, 820, 1022
DpnII GATC 6 cut(s) 148, 427, 603, 746, 818, 1020
Eam1104I CTCTTC 3 cut(s) 287, 325, 753
EarI CTCTTC 3 cut(s) 287, 325, 753
EciI GGCGGA 1 cut(s) 913
Eco130I CCWWGG 1 cut(s) 633
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 2 cut(s) 326, 784
Eco57I CTGAAG 1 cut(s) 715
EcoRII CCWGG 3 cut(s) 469, 529, 1055
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 1 cut(s) 633
ErhI CCWWGG 1 cut(s) 633
FaeI CATG 4 cut(s) 169, 374, 513, 1029
FalI AAGNNNNNCTT 4 cut(s) 692, 724, 1001, 1033
FaqI GGGAC 1 cut(s) 660
FatI CATG 4 cut(s) 165, 370, 509, 1025
FbaI TGATCA 1 cut(s) 1020
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 2 cut(s) 222, 266
FokI GGATG 2 cut(s) 327, 353
Fsp4HI GCNGC 2 cut(s) 222, 266
FspBI CTAG 2 cut(s) 273, 585
GluI GCNGC 2 cut(s) 222, 266
GsuI CTGGAG 1 cut(s) 97
HaeIII GGCC 1 cut(s) 508
HapII CCGG 3 cut(s) 324, 435, 771
Hin1II CATG 4 cut(s) 169, 374, 513, 1029
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HpaII CCGG 3 cut(s) 324, 435, 771
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 2 cut(s) 52, 73
Hpy188I TCNGA 7 cut(s) 102, 289, 352, 383, 610, 734, 970
Hpy188III TCNNGA 3 cut(s) 324, 421, 750
Hpy8I GTNNAC 2 cut(s) 52, 73
HpyAV CCTTC 6 cut(s) 347, 414, 427, 605, 632, 895
HpyCH4III ACNGT 5 cut(s) 247, 293, 395, 501, 932
HpyCH4V TGCA 2 cut(s) 197, 918
HpyF10VI GCNNNNNNNGC 1 cut(s) 810
HpyF3I CTNAG 1 cut(s) 609
Hsp92II CATG 4 cut(s) 169, 374, 513, 1029
Kpn2I TCCGGA 1 cut(s) 323
Ksp22I TGATCA 1 cut(s) 1020
Kzo9I GATC 6 cut(s) 148, 427, 603, 746, 818, 1020
LguI GCTCTTC 2 cut(s) 325, 753
LmnI GCTCC 4 cut(s) 78, 619, 712, 801
Lsp1109I GCAGC 2 cut(s) 208, 277
LweI GCATC 1 cut(s) 305
MabI ACCWGGT 1 cut(s) 1055
MaeI CTAG 2 cut(s) 273, 585
MalI GATC 6 cut(s) 150, 429, 605, 748, 820, 1022
MboI GATC 6 cut(s) 148, 427, 603, 746, 818, 1020
MflI RGATCY 1 cut(s) 603
MhlI GDGCHC 1 cut(s) 83
MluCI AATT 7 cut(s) 64, 96, 562, 716, 832, 964, 1038
MnlI CCTC 9 cut(s) 136, 167, 484, 604, 637, 767, 797, 870, 888
MroI TCCGGA 1 cut(s) 323
MroXI GAANNNNTTC 1 cut(s) 281
MseI TTAA 3 cut(s) 6, 11, 249
MslI CAYNNNNRTG 2 cut(s) 168, 1030
MspCI CTTAAG 1 cut(s) 5
MspI CCGG 3 cut(s) 324, 435, 771
MspR9I CCNGG 5 cut(s) 435, 471, 531, 771, 1057
Mva1269I GAATGC 1 cut(s) 531
MvaI CCWGG 3 cut(s) 471, 531, 1057
MwoI GCNNNNNNNGC 1 cut(s) 810
NciI CCSGG 2 cut(s) 435, 771
NdeII GATC 6 cut(s) 148, 427, 603, 746, 818, 1020
NlaIII CATG 4 cut(s) 169, 374, 513, 1029
NlaIV GGNNCC 3 cut(s) 328, 615, 768
PaqCI CACCTGC 1 cut(s) 697
PciSI GCTCTTC 2 cut(s) 325, 753
PctI GAATGC 1 cut(s) 531
PdmI GAANNNNTTC 1 cut(s) 281
PfoI TCCNGGA 2 cut(s) 433, 769
PkrI GCNGC 2 cut(s) 223, 267
Psp6I CCWGG 3 cut(s) 469, 529, 1055
PspGI CCWGG 3 cut(s) 469, 529, 1055
PspN4I GGNNCC 3 cut(s) 328, 615, 768
PspPI GGNCC 3 cut(s) 326, 506, 784
PsuI RGATCY 1 cut(s) 603
RsaI GTAC 1 cut(s) 707
RsaNI GTAC 1 cut(s) 706
RseI CAYNNNNRTG 2 cut(s) 168, 1030
SapI GCTCTTC 2 cut(s) 325, 753
SaqAI TTAA 3 cut(s) 6, 11, 249
SatI GCNGC 2 cut(s) 222, 266
Sau3AI GATC 6 cut(s) 148, 427, 603, 746, 818, 1020
Sau96I GGNCC 3 cut(s) 326, 506, 784
ScaI AGTACT 1 cut(s) 707
ScrFI CCNGG 5 cut(s) 435, 471, 531, 771, 1057
SduI GDGCHC 1 cut(s) 83
SexAI ACCWGGT 1 cut(s) 1055
SfaNI GCATC 1 cut(s) 305
SinI GGWCC 2 cut(s) 326, 784
SmiMI CAYNNNNRTG 2 cut(s) 168, 1030
SmlI CTYRAG 2 cut(s) 5, 488
SmoI CTYRAG 2 cut(s) 5, 488
Sse9I AATT 7 cut(s) 64, 96, 562, 716, 832, 964, 1038
SsiI CCGC 1 cut(s) 898
SspI AATATT 1 cut(s) 119
SspMI CTAG 2 cut(s) 273, 585
StyD4I CCNGG 5 cut(s) 433, 469, 529, 769, 1055
StyI CCWWGG 1 cut(s) 633
TaaI ACNGT 5 cut(s) 247, 293, 395, 501, 932
TaqI TCGA 2 cut(s) 817, 893
TasI AATT 7 cut(s) 64, 96, 562, 716, 832, 964, 1038
TatI WGTACW 1 cut(s) 705
Tru1I TTAA 3 cut(s) 6, 11, 249
Tru9I TTAA 3 cut(s) 6, 11, 249
TscAI CASTG 1 cut(s) 937
TseI GCWGC 2 cut(s) 221, 265
TspDTI ATGAA 3 cut(s) 182, 505, 1038
TspGWI ACGGA 1 cut(s) 58
TspRI CASTG 1 cut(s) 937
Vha464I CTTAAG 1 cut(s) 5
VpaK11BI GGWCC 2 cut(s) 326, 784
XcmI CCANNNNNNNNNTGG 2 cut(s) 29, 337
XmiI GTMKAC 1 cut(s) 72
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 2 cut(s) 273, 585
ZrmI AGTACT 1 cut(s) 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.