FvH4_3g03130

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000342
Physical Location & Seq
Reverse (-)
1678430 .. 1680235
1806 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03130.t1

Sequence Viewer

Length: 1101 bp
ATGGAGGTCGAGCAGGTTCTTCACATGAATGGAGGAGATGGCAAAACAAGCTACGCAAATCACTCACTTCTTCAAAGAGCTGTGATTTCGACGGTGAAGCCCATAGTGGACGCAAGCATAGAGGACCTCTGCAACACTCTCTTCCCAGAGTGTCTGAAAATAGCAGACTTGGGGTGCTCTTCCGGACCCAATACCCTTCTGGTGGTATCAGACATCATTGAAAATAGCCGAAACACGTTTCAAAAGCTCAACCTCCCCCCGCCATCGCTCCAAGCATTCTTGAATGATCTTCCCTGGAATGATTTCAACACGGTGTTCAGGTCACTGCCTGGCTTCTATAAGAAGCTCCATGAAGAAAATGGGAACAAGTCGGGTTCTTGTTTCATTGCAGCAATGCCTGGTTCCTTTTATGGCAGGCTCTTTCCTGACAACTCTCTTCACTTTGTTCATTCTTCTTATGCTCTCATGTGGATCTCTGAGGTTCCAAAAGGTCTGGTAACCGAAGGAGGAGAGGGACTGAACAAAGGGAACATATACATATCCAAGACAAGCTCACCTGCTGTGTTTAATGAATACTTTGAGCAATTCAAAAGGGACTTCACAGTCTTTCTGCGATCTAGGGCACACGAGCTAGTCCCGGGAGGTAGTATGGTCCTCACAACCATGGGCAGCATAAAGAGCAATGATCCCCTCTCCATTTGGGAATTCGTCGGATTGAAACTCCATGACATGGTTTTAGATGGTTTGATTGAGGAGAAAAAGTTGGACAGATTCAATTTGCCATACTATGCACCCACAACAGATGAGGTAAAAGAGGTGATCGAGGCTGAAGGGTCTTTTACTTTGCAAAACCTTGAAGCTTTCAAAAATGACTGGGATTCTTACATAAAACAAGCTCATAGTGACCTTGACAAGAAAGCAAGGGCTGCAATACTCTCCACTGACATAAGGGCAGTGGGAGAGCCTATTCTGGCTAGCCAATTTGGAGAAGAAGCAGTGGACGAATTGTTCCGTAAGTTCGAAGAAAATGTTCTTGATCATATGGAAAGGGAGAAGTGTCAGTTCATTAACCTAGTTATATCATTGATCAAGAAGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

367

Amino Acids

41.13

Weight (kDa)

5.24

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 40 - 364 1.5e-120 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14060 AT2G14060 AT3G11480 AT3G21950 AT3G21950 AT5G04370 AT5G04370 AT5G04370 AT5G04380 AT5G04380 AT5G04380 AT5G38020 AT5G38020 AT5G66430
fragaria_vesca FvH4_2g09290 FvH4_2g09290 FvH4_2g09310 FvH4_3g03130
malus_domestica MD10G1071000.v1.1 MD10G1071200.v1.1 MD10G1071500.v1.1 MD10G1218400.v1.1
prunus_persica Prupe.8G092700_v2.0.a1 Prupe.8G092800_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G093000_v2.0.a1 Prupe.8G093100_v2.0.a1 Prupe.8G093200_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093600_v2.0.a1
pyrus_communis pycom10g05660 pycom10g05680 pycom10g18420
rosa_chinensis RchiOBHm_Chr1g0324421 RchiOBHm_Chr1g0324461 RchiOBHm_Chr6g0265311 RchiOBHm_Chr6g0265321 RchiOBHm_Chr6g0265361 RchiOBHm_Chr6g0265391 RchiOBHm_Chr6g0265411
rosa_laevigata RLG00000014186 RLG00000014191 RLG00000014195 RLG00000014197 RLG00000025381 RLG00000030199
rosa_multiflora Rmu_co8237181.1_g000001 Rmu_sc0001780.1_g000008 Rmu_sc0001803.1_g000020 Rmu_sc0002550.1_g000004 Rmu_sc0004082.1_g000012 Rmu_sc0008601.1_g000002 Rmu_sc0011954.1_g000001 Rmu_sc0013000.1_g000001 Rmu_sc0016482.1_g000001 Rmu_sc0018713.1_g000002 Rmu_ssc0000066.1_g000015
rosa_roxburghii Rroxscaffold_159G00432720 Rroxscaffold_159G00432790 Rroxscaffold_159G00432850 Rroxscaffold_175G00432320 Rroxscaffold_175G00432340 Rroxscaffold_175G00432350 Rroxscaffold_175G00432360 Rroxscaffold_2G00105600 Rroxscaffold_2G00105660 Rroxscaffold_4G00325530 Rroxscaffold_4G00325600 Rroxscaffold_4G00325670 Rroxscaffold_6G00424510 Rroxscaffold_7G00202680 Rroxscaffold_7G00202760 Rroxscaffold_7G00202800 Rroxscaffold_7G00202850
rosa_rugosa Rorug01G0045900 Rorug01G0046000 Rorug02G0355000 Rorug02G0355300 Rorug03G0004200 Rorug06G0016700 Rorug06G0016700 Rorug06G0016800 Rorug06G0017100
rosa_samantha Rh1AG061600 Rh1AG061700 Rh1BG052400 Rh1CG063500 Rh1CG063900 Rh1CG064100 Rh2BG415800 Rh3DG067100 Rh6AG138200 Rh6AG138400 Rh6AG138800 Rh6BG137100 Rh6BG137200 Rh6BG137600 Rh6DG121600 Rh6DG121700 Rh6DG121900
rosa_wichuraiana Rw1G005230 Rw1G005470 Rw2G033110 Rw6G011920 Rw6G011940 Rw6G011950 Rw6G011970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 565
AasI GACNNNNNNGTC 1 cut(s) 602
Acc36I ACCTGC 2 cut(s) 4, 565
AccB7I CCANNNNNTGG 1 cut(s) 730
AccIII TCCGGA 1 cut(s) 182
AciI CCGC 1 cut(s) 260
AclWI GGATC 2 cut(s) 479, 680
AcsI RAATTY 1 cut(s) 704
AcuI CTGAAG 1 cut(s) 849
AfiI CCNNNNNNNGG 2 cut(s) 202, 730
AflIII ACRYGT 1 cut(s) 234
AjnI CCWGG 3 cut(s) 293, 328, 397
AloI GAACNNNNNNTCC 2 cut(s) 992, 1024
AluBI AGCT 8 cut(s) 51, 80, 247, 346, 552, 631, 860, 896
AluI AGCT 8 cut(s) 51, 80, 247, 346, 552, 631, 860, 896
Alw21I GWGCWC 1 cut(s) 179
AlwI GGATC 2 cut(s) 479, 680
Ama87I CYCGRG 1 cut(s) 637
Aor13HI TCCGGA 1 cut(s) 182
ApeKI GCWGC 3 cut(s) 389, 669, 926
ApoI RAATTY 1 cut(s) 704
Asp700I GAANNNNTTC 2 cut(s) 302, 1029
AspS9I GGNCC 3 cut(s) 124, 185, 652
AsuC2I CCSGG 2 cut(s) 638, 639
AsuHPI GGTGA 3 cut(s) 106, 546, 829
AsuII TTCGAA 1 cut(s) 1020
AsuNHI GCTAGC 1 cut(s) 974
AvaI CYCGRG 1 cut(s) 637
AvaII GGWCC 3 cut(s) 124, 185, 652
BaeGI GKGCMC 1 cut(s) 625
BauI CACGAG 1 cut(s) 626
Bbv12I GWGCWC 1 cut(s) 179
BbvI GCAGC 3 cut(s) 401, 681, 913
BccI CCATC 3 cut(s) 32, 271, 734
BciT130I CCWGG 3 cut(s) 295, 330, 399
BclI TGATCA 2 cut(s) 1036, 1086
BcnI CCSGG 2 cut(s) 638, 639
BfaI CTAG 4 cut(s) 618, 632, 975, 1073
BfuAI ACCTGC 2 cut(s) 4, 565
BisI GCNGC 3 cut(s) 390, 670, 927
BlsI GCNGC 3 cut(s) 391, 671, 928
Bme1390I CCNGG 5 cut(s) 295, 330, 399, 638, 639
Bme18I GGWCC 3 cut(s) 124, 185, 652
BmeT110I CYCGRG 1 cut(s) 637
BmgT120I GGNCC 3 cut(s) 124, 185, 652
BmiI GGNNCC 3 cut(s) 187, 403, 483
BmrFI CCNGG 5 cut(s) 295, 330, 399, 638, 639
BmrI ACTGGG 1 cut(s) 883
BmtI GCTAGC 1 cut(s) 978
BmuI ACTGGG 1 cut(s) 883
Bpu14I TTCGAA 1 cut(s) 1020
BpuMI CCSGG 2 cut(s) 638, 639
BsaJI CCNNGG 3 cut(s) 293, 637, 663
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 2 cut(s) 746, 776
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 730
Bse1I ACTGG 1 cut(s) 878
Bse3DI GCAATG 3 cut(s) 384, 399, 688
BseAI TCCGGA 1 cut(s) 182
BseBI CCWGG 3 cut(s) 295, 330, 399
BseDI CCNNGG 3 cut(s) 293, 637, 663
BseLI CCNNNNNNNGG 2 cut(s) 202, 730
BseMI GCAATG 3 cut(s) 384, 399, 688
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 1 cut(s) 878
BseRI GAGGAG 3 cut(s) 48, 522, 767
BseSI GKGCMC 1 cut(s) 625
BseXI GCAGC 3 cut(s) 401, 681, 913
BsiHKAI GWGCWC 1 cut(s) 179
BsiHKCI CYCGRG 1 cut(s) 637
BsiSI CCGG 2 cut(s) 183, 638
BslFI GGGAC 3 cut(s) 528, 608, 620
BslI CCNNNNNNNGG 2 cut(s) 202, 730
BsmFI GGGAC 3 cut(s) 528, 608, 620
BsmI GAATGC 1 cut(s) 275
BsoBI CYCGRG 1 cut(s) 637
Bsp119I TTCGAA 1 cut(s) 1020
Bsp1286I GDGCHC 2 cut(s) 179, 625
Bsp13I TCCGGA 1 cut(s) 182
Bsp143I GATC 7 cut(s) 286, 471, 614, 685, 819, 1036, 1086
Bsp19I CCATGG 1 cut(s) 663
BspACI CCGC 1 cut(s) 260
BspCNI CTCAG 1 cut(s) 469
BspEI TCCGGA 1 cut(s) 182
BspLI GGNNCC 3 cut(s) 187, 403, 483
BspMI ACCTGC 2 cut(s) 4, 565
BspOI GCTAGC 1 cut(s) 978
BspPI GGATC 2 cut(s) 479, 680
BspQI GCTCTTC 1 cut(s) 184
BspT104I TTCGAA 1 cut(s) 1020
BsrDI GCAATG 3 cut(s) 384, 399, 688
BsrI ACTGG 1 cut(s) 878
BssECI CCNNGG 3 cut(s) 293, 637, 663
BssMI GATC 7 cut(s) 286, 471, 614, 685, 819, 1036, 1086
BssSI CACGAG 1 cut(s) 626
BssT1I CCWWGG 1 cut(s) 663
Bst2BI CACGAG 1 cut(s) 626
Bst2UI CCWGG 3 cut(s) 295, 330, 399
Bst4CI ACNGT 3 cut(s) 94, 313, 604
Bst6I CTCTTC 3 cut(s) 146, 184, 441
BstAPI GCANNNNNTGC 1 cut(s) 926
BstBI TTCGAA 1 cut(s) 1020
BstC8I GCNNGC 3 cut(s) 115, 416, 976
BstDEI CTNAG 1 cut(s) 477
BstDSI CCRYGG 1 cut(s) 663
BstEII GGTNACC 1 cut(s) 496
BstKTI GATC 7 cut(s) 289, 474, 617, 688, 822, 1039, 1089
BstMBI GATC 7 cut(s) 286, 471, 614, 685, 819, 1036, 1086
BstMWI GCNNNNNNNGC 3 cut(s) 48, 678, 926
BstNI CCWGG 3 cut(s) 295, 330, 399
BstPI GGTNACC 1 cut(s) 496
BstSCI CCNGG 5 cut(s) 293, 328, 397, 636, 637
BstSLI GKGCMC 1 cut(s) 625
BstV1I GCAGC 3 cut(s) 401, 681, 913
BstX2I RGATCY 1 cut(s) 471
BstYI RGATCY 1 cut(s) 471
BtgI CCRYGG 1 cut(s) 663
BtgZI GCGATG 1 cut(s) 249
BtsI GCAGTG 3 cut(s) 323, 960, 1002
BtsIMutI CAGTG 4 cut(s) 323, 939, 960, 1002
BveI ACCTGC 2 cut(s) 4, 565
Cac8I GCNNGC 3 cut(s) 115, 416, 976
Cfr13I GGNCC 3 cut(s) 124, 185, 652
Cfr9I CCCGGG 1 cut(s) 637
CseI GACGC 1 cut(s) 119
CviAII CATG 6 cut(s) 25, 350, 466, 664, 725, 730
DdeI CTNAG 1 cut(s) 477
DpnI GATC 7 cut(s) 288, 473, 616, 687, 821, 1038, 1088
DpnII GATC 7 cut(s) 286, 471, 614, 685, 819, 1036, 1086
DrdI GACNNNNNNGTC 1 cut(s) 602
DseDI GACNNNNNNGTC 1 cut(s) 602
Eam1104I CTCTTC 3 cut(s) 146, 184, 441
EarI CTCTTC 3 cut(s) 146, 184, 441
Eco130I CCWWGG 1 cut(s) 663
Eco47I GGWCC 3 cut(s) 124, 185, 652
Eco57I CTGAAG 1 cut(s) 849
Eco88I CYCGRG 1 cut(s) 637
Eco91I GGTNACC 1 cut(s) 496
EcoO109I RGGNCCY 1 cut(s) 124
EcoO65I GGTNACC 1 cut(s) 496
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 3 cut(s) 293, 328, 397
EcoT14I CCWWGG 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 663
FaeI CATG 6 cut(s) 28, 353, 469, 667, 728, 733
FaqI GGGAC 3 cut(s) 528, 608, 620
FatI CATG 6 cut(s) 24, 349, 465, 663, 724, 729
FauI CCCGC 1 cut(s) 267
FauNDI CATATG 1 cut(s) 1041
FbaI TGATCA 2 cut(s) 1036, 1086
Fnu4HI GCNGC 3 cut(s) 390, 670, 927
Fsp4HI GCNGC 3 cut(s) 390, 670, 927
FspBI CTAG 4 cut(s) 618, 632, 975, 1073
GluI GCNGC 3 cut(s) 390, 670, 927
HapII CCGG 2 cut(s) 183, 638
HgaI GACGC 1 cut(s) 119
Hin1II CATG 6 cut(s) 28, 353, 469, 667, 728, 733
HindIII AAGCTT 1 cut(s) 858
HinfI GANTC 2 cut(s) 771, 878
HpaII CCGG 2 cut(s) 183, 638
HphI GGTGA 3 cut(s) 106, 546, 829
Hpy166II GTNNAC 2 cut(s) 109, 1000
Hpy188I TCNGA 4 cut(s) 156, 211, 478, 713
Hpy188III TCNNGA 5 cut(s) 183, 280, 425, 1034, 1090
Hpy8I GTNNAC 2 cut(s) 109, 1000
Hpy99I CGWCG 2 cut(s) 94, 713
HpyAV CCTTC 3 cut(s) 206, 497, 824
HpyCH4III ACNGT 3 cut(s) 94, 313, 604
HpyCH4IV ACGT 1 cut(s) 236
HpyCH4V TGCA 5 cut(s) 132, 389, 791, 847, 929
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 678, 926
HpyF3I CTNAG 1 cut(s) 477
HpySE526I ACGT 1 cut(s) 236
Hsp92II CATG 6 cut(s) 28, 353, 469, 667, 728, 733
Kpn2I TCCGGA 1 cut(s) 182
Ksp22I TGATCA 2 cut(s) 1036, 1086
Kzo9I GATC 7 cut(s) 286, 471, 614, 685, 819, 1036, 1086
LguI GCTCTTC 1 cut(s) 184
LmnI GCTCC 2 cut(s) 273, 351
Lsp1109I GCAGC 3 cut(s) 401, 681, 913
MaeI CTAG 4 cut(s) 618, 632, 975, 1073
MaeII ACGT 1 cut(s) 236
MaeIII GTNAC 3 cut(s) 321, 496, 902
MalI GATC 7 cut(s) 288, 473, 616, 687, 821, 1038, 1088
MboI GATC 7 cut(s) 286, 471, 614, 685, 819, 1036, 1086
MflI RGATCY 1 cut(s) 471
MhlI GDGCHC 2 cut(s) 179, 625
MluCI AATT 5 cut(s) 584, 704, 775, 980, 1004
MmeI TCCRAC 2 cut(s) 691, 744
MroI TCCGGA 1 cut(s) 182
MroXI GAANNNNTTC 2 cut(s) 302, 1029
MseI TTAA 3 cut(s) 567, 1068, 1099
MslI CAYNNNNRTG 2 cut(s) 27, 662
MspI CCGG 2 cut(s) 183, 638
MspR9I CCNGG 5 cut(s) 295, 330, 399, 638, 639
Mva1269I GAATGC 1 cut(s) 275
MvaI CCWGG 3 cut(s) 295, 330, 399
MwoI GCNNNNNNNGC 3 cut(s) 48, 678, 926
NciI CCSGG 2 cut(s) 638, 639
NcoI CCATGG 1 cut(s) 663
NdeI CATATG 1 cut(s) 1041
NdeII GATC 7 cut(s) 286, 471, 614, 685, 819, 1036, 1086
NheI GCTAGC 1 cut(s) 974
NlaIII CATG 6 cut(s) 28, 353, 469, 667, 728, 733
NlaIV GGNNCC 3 cut(s) 187, 403, 483
NmuCI GTSAC 2 cut(s) 321, 902
NspV TTCGAA 1 cut(s) 1020
PaqCI CACCTGC 1 cut(s) 565
PciSI GCTCTTC 1 cut(s) 184
PctI GAATGC 1 cut(s) 275
PdmI GAANNNNTTC 2 cut(s) 302, 1029
PfeI GAWTC 2 cut(s) 771, 878
PflMI CCANNNNNTGG 1 cut(s) 730
PkrI GCNGC 3 cut(s) 391, 671, 928
PpuMI RGGWCCY 1 cut(s) 124
Psp5II RGGWCCY 1 cut(s) 124
Psp6I CCWGG 3 cut(s) 293, 328, 397
PspEI GGTNACC 1 cut(s) 496
PspGI CCWGG 3 cut(s) 293, 328, 397
PspN4I GGNNCC 3 cut(s) 187, 403, 483
PspPI GGNCC 3 cut(s) 124, 185, 652
PspPPI RGGWCCY 1 cut(s) 124
PsuI RGATCY 1 cut(s) 471
RseI CAYNNNNRTG 2 cut(s) 27, 662
SapI GCTCTTC 1 cut(s) 184
SaqAI TTAA 3 cut(s) 567, 1068, 1099
SatI GCNGC 3 cut(s) 390, 670, 927
Sau3AI GATC 7 cut(s) 286, 471, 614, 685, 819, 1036, 1086
Sau96I GGNCC 3 cut(s) 124, 185, 652
ScrFI CCNGG 5 cut(s) 295, 330, 399, 638, 639
SduI GDGCHC 2 cut(s) 179, 625
SfuI TTCGAA 1 cut(s) 1020
SinI GGWCC 3 cut(s) 124, 185, 652
SmaI CCCGGG 1 cut(s) 639
SmiMI CAYNNNNRTG 2 cut(s) 27, 662
Sse9I AATT 5 cut(s) 584, 704, 775, 980, 1004
SsiI CCGC 1 cut(s) 260
SspMI CTAG 4 cut(s) 618, 632, 975, 1073
StyD4I CCNGG 5 cut(s) 293, 328, 397, 636, 637
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 3 cut(s) 94, 313, 604
TaiI ACGT 1 cut(s) 239
TaqI TCGA 4 cut(s) 9, 89, 822, 1020
TasI AATT 5 cut(s) 584, 704, 775, 980, 1004
TfiI GAWTC 2 cut(s) 771, 878
Tru1I TTAA 3 cut(s) 567, 1068, 1099
Tru9I TTAA 3 cut(s) 567, 1068, 1099
TscAI CASTG 4 cut(s) 330, 946, 960, 1002
TseFI GTSAC 2 cut(s) 321, 902
TseI GCWGC 3 cut(s) 389, 669, 926
Tsp45I GTSAC 2 cut(s) 321, 902
TspDTI ATGAA 6 cut(s) 41, 366, 373, 437, 585, 1054
TspGWI ACGGA 1 cut(s) 1001
TspMI CCCGGG 1 cut(s) 637
TspRI CASTG 4 cut(s) 330, 946, 960, 1002
Van91I CCANNNNNTGG 1 cut(s) 730
VpaK11BI GGWCC 3 cut(s) 124, 185, 652
XapI RAATTY 1 cut(s) 704
XcmI CCANNNNNNNNNTGG 2 cut(s) 196, 356
XmaI CCCGGG 1 cut(s) 637
XmnI GAANNNNTTC 2 cut(s) 302, 1029
XspI CTAG 4 cut(s) 618, 632, 975, 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.