AT5G66430

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000342
Physical Location & Seq
Reverse (-)
26525410 .. 26526799
1390 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66430.1

Sequence Viewer

Length: 1065 bp
ATGAGCGGTGGAGATGGAGATAATAGTTACTCCACCAATTCTCTTCTTCAGAAAAAAGTATTATCAAAGGCCAAACCGGTTTTGGTTAAGAACACAAAAGGAATGATGATAAACTTAAATTTTCCTAATTACATCAAAGTAGCTGATTTGGGATGTGCTACTGGAGAAAACACGTTCTTGACGATGGCTGAGATCGTGAACACGATCAATGTGTTATGTCAACAATGTAACCAAAAGCCACCAGAAATTGATTGCTGTCTGAATGATCTTCCGGATAACGATTTCAACACGACATTCAAGTTCGTACCTTTCTTCAATAAAAGGGTCAAAAGCAAAAGATTGTGCTTCGTGTCTGGAGTCCCCGGTTCATTCTACTCGAGGCTCTTTCCTCGCAAAAGTCTCCATTTTGTACATTCATCTTATAGCCTCCATTGGCTCTCTAAGGTTCCCAAAGGACTGGAGAAGAATTCCTCGTCTGTGTACATAACGACTTCAAGTCCTCCAAATGCATACAAGGCTTATTTAAATCAATTCCAAAGTGACTTTAAGTCATTTCTAGAAATGCGTTCTGAGGAGATGGTTTCTAATGGACGCATGGTTCTCACTTTCATCGGTAGAAAGACTCTCGATGATCCATTGCATAGAGATTGTTGTCACTTTTGGACACTATTATCTACATCTCTACGCGACCTAGTCTATGAGGGTCTTGTGAGTGCGTCAAAGGTGGACTCTTTCAACATACCGTTTTATGATCCGAGCAAAGAAGAAGTGATGGAGATGATTCGAAACGAAGGCTCATTCGAGATCAATGACTTGGAGATTCATGGATTTGAGCTTGGCCTAAGCAACCATGACGAGGACTACATGTTACATTCACAAATATCTAAAGCAGGGCAGAGGGAAGCCAACTGTATAAGAGCGGTCAGCGAATCAATGCTTGTAGCTGACTTCGGAGTAGACATTATGGACACATTGTTTAAGAAATTTGCATACCATGTGTCACAACATGCTAGCTGCACGAACAAAACGACTGTTACTCTAGTCGTTTCTTTGATACGAAAGTAA

Protein Analysis

354

Amino Acids

40.03

Weight (kDa)

7.56

Isoelectric Point (pI)

35.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 32 - 353 2.1e-124 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14060 AT2G14060 AT3G11480 AT3G21950 AT3G21950 AT5G04370 AT5G04370 AT5G04370 AT5G04380 AT5G04380 AT5G04380 AT5G38020 AT5G38020 AT5G66430
fragaria_vesca FvH4_2g09290 FvH4_2g09290 FvH4_2g09310 FvH4_3g03130
malus_domestica MD10G1071000.v1.1 MD10G1071200.v1.1 MD10G1071500.v1.1 MD10G1218400.v1.1
prunus_persica Prupe.8G092700_v2.0.a1 Prupe.8G092800_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G093000_v2.0.a1 Prupe.8G093100_v2.0.a1 Prupe.8G093200_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093600_v2.0.a1
pyrus_communis pycom10g05660 pycom10g05680 pycom10g18420
rosa_chinensis RchiOBHm_Chr1g0324421 RchiOBHm_Chr1g0324461 RchiOBHm_Chr6g0265311 RchiOBHm_Chr6g0265321 RchiOBHm_Chr6g0265361 RchiOBHm_Chr6g0265391 RchiOBHm_Chr6g0265411
rosa_laevigata RLG00000014186 RLG00000014191 RLG00000014195 RLG00000014197 RLG00000025381 RLG00000030199
rosa_multiflora Rmu_co8237181.1_g000001 Rmu_sc0001780.1_g000008 Rmu_sc0001803.1_g000020 Rmu_sc0002550.1_g000004 Rmu_sc0004082.1_g000012 Rmu_sc0008601.1_g000002 Rmu_sc0011954.1_g000001 Rmu_sc0013000.1_g000001 Rmu_sc0016482.1_g000001 Rmu_sc0018713.1_g000002 Rmu_ssc0000066.1_g000015
rosa_roxburghii Rroxscaffold_159G00432720 Rroxscaffold_159G00432790 Rroxscaffold_159G00432850 Rroxscaffold_175G00432320 Rroxscaffold_175G00432340 Rroxscaffold_175G00432350 Rroxscaffold_175G00432360 Rroxscaffold_2G00105600 Rroxscaffold_2G00105660 Rroxscaffold_4G00325530 Rroxscaffold_4G00325600 Rroxscaffold_4G00325670 Rroxscaffold_6G00424510 Rroxscaffold_7G00202680 Rroxscaffold_7G00202760 Rroxscaffold_7G00202800 Rroxscaffold_7G00202850
rosa_rugosa Rorug01G0045900 Rorug01G0046000 Rorug02G0355000 Rorug02G0355300 Rorug03G0004200 Rorug06G0016700 Rorug06G0016700 Rorug06G0016800 Rorug06G0017100
rosa_samantha Rh1AG061600 Rh1AG061700 Rh1BG052400 Rh1CG063500 Rh1CG063900 Rh1CG064100 Rh2BG415800 Rh3DG067100 Rh6AG138200 Rh6AG138400 Rh6AG138800 Rh6BG137100 Rh6BG137200 Rh6BG137600 Rh6DG121600 Rh6DG121700 Rh6DG121900
rosa_wichuraiana Rw1G005230 Rw1G005470 Rw2G033110 Rw6G011920 Rw6G011940 Rw6G011950 Rw6G011970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 6, 920
AccI GTMKAC 1 cut(s) 957
AccII CGCG 1 cut(s) 687
AccIII TCCGGA 1 cut(s) 271
AciI CCGC 2 cut(s) 6, 920
AclWI GGATC 2 cut(s) 626, 746
AcsI RAATTY 3 cut(s) 118, 466, 983
AcuI CTGAAG 1 cut(s) 32
AfaI GTAC 3 cut(s) 306, 411, 482
AfiI CCNNNNNNNGG 1 cut(s) 856
AflIII ACRYGT 2 cut(s) 171, 864
AgeI ACCGGT 1 cut(s) 76
AgsI TTSAA 5 cut(s) 286, 298, 316, 495, 736
AhdI GACNNNNNGTC 2 cut(s) 495, 547
AloI GAACNNNNNNTCC 2 cut(s) 582, 614
AluBI AGCT 4 cut(s) 143, 835, 944, 1014
AluI AGCT 4 cut(s) 143, 835, 944, 1014
Alw26I GTCTC 1 cut(s) 404
AlwI GGATC 2 cut(s) 626, 746
Ama87I CYCGRG 1 cut(s) 376
Aor13HI TCCGGA 1 cut(s) 271
AoxI GGCC 2 cut(s) 69, 838
ApeKI GCWGC 1 cut(s) 1014
ApoI RAATTY 3 cut(s) 118, 466, 983
AsiGI ACCGGT 1 cut(s) 76
AsuC2I CCSGG 1 cut(s) 363
AsuII TTCGAA 1 cut(s) 784
AsuNHI GCTAGC 1 cut(s) 1010
AvaI CYCGRG 1 cut(s) 376
BaeI ACNNNNGTAYC 1 cut(s) 1046
BbvI GCAGC 1 cut(s) 1001
BccI CCATC 4 cut(s) 8, 178, 571, 766
BcnI CCSGG 1 cut(s) 363
BcoDI GTCTC 1 cut(s) 404
BfaI CTAG 4 cut(s) 557, 692, 1011, 1040
BisI GCNGC 1 cut(s) 1015
BlsI GCNGC 1 cut(s) 1016
Bme1390I CCNGG 1 cut(s) 363
BmeRI GACNNNNNGTC 2 cut(s) 495, 547
BmeT110I CYCGRG 1 cut(s) 376
BmiI GGNNCC 1 cut(s) 447
BmrFI CCNGG 1 cut(s) 363
BmtI GCTAGC 1 cut(s) 1014
BpmI CTGGAG 3 cut(s) 183, 375, 479
Bpu10I CCTNAGC 1 cut(s) 842
Bpu14I TTCGAA 1 cut(s) 784
BpuMI CCSGG 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 361
BsaWI WCCGGW 2 cut(s) 76, 271
Bsc4I CCNNNNNNNGG 1 cut(s) 856
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 2 cut(s) 166, 462
Bse3DI GCAATG 1 cut(s) 635
BseAI TCCGGA 1 cut(s) 271
BseDI CCNNGG 1 cut(s) 361
BseGI GGATG 1 cut(s) 158
BseLI CCNNNNNNNGG 1 cut(s) 856
BseMI GCAATG 1 cut(s) 635
BseMII CTCAG 2 cut(s) 180, 561
BseNI ACTGG 2 cut(s) 166, 462
BseRI GAGGAG 1 cut(s) 587
BseXI GCAGC 1 cut(s) 1001
BsgI GTGCAG 1 cut(s) 1000
Bsh1236I CGCG 1 cut(s) 687
BshFI GGCC 2 cut(s) 71, 840
BshTI ACCGGT 1 cut(s) 76
BsiHKCI CYCGRG 1 cut(s) 376
BsiSI CCGG 3 cut(s) 77, 272, 363
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 1 cut(s) 856
BsmAI GTCTC 1 cut(s) 404
BsmFI GGGAC 1 cut(s) 344
BsnI GGCC 2 cut(s) 71, 840
BsoBI CYCGRG 1 cut(s) 376
Bsp119I TTCGAA 1 cut(s) 784
Bsp13I TCCGGA 1 cut(s) 271
Bsp1407I TGTACA 2 cut(s) 409, 480
Bsp143I GATC 6 cut(s) 192, 204, 265, 631, 751, 804
BspACI CCGC 2 cut(s) 6, 920
BspANI GGCC 2 cut(s) 71, 840
BspCNI CTCAG 2 cut(s) 181, 562
BspEI TCCGGA 1 cut(s) 271
BspFNI CGCG 1 cut(s) 687
BspLI GGNNCC 1 cut(s) 447
BspOI GCTAGC 1 cut(s) 1014
BspPI GGATC 2 cut(s) 626, 746
BspT104I TTCGAA 1 cut(s) 784
BsrBI CCGCTC 2 cut(s) 6, 920
BsrDI GCAATG 1 cut(s) 635
BsrFI RCCGGY 1 cut(s) 76
BsrGI TGTACA 2 cut(s) 409, 480
BsrI ACTGG 2 cut(s) 166, 462
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 361
BssMI GATC 6 cut(s) 192, 204, 265, 631, 751, 804
Bst4CI ACNGT 3 cut(s) 744, 911, 1033
Bst6I CTCTTC 1 cut(s) 48
BstAUI TGTACA 2 cut(s) 409, 480
BstBI TTCGAA 1 cut(s) 784
BstC8I GCNNGC 1 cut(s) 1012
BstDEI CTNAG 4 cut(s) 189, 441, 570, 842
BstF5I GGATG 1 cut(s) 158
BstFNI CGCG 1 cut(s) 687
BstKTI GATC 6 cut(s) 195, 207, 268, 634, 754, 807
BstMAI GTCTC 1 cut(s) 404
BstMBI GATC 6 cut(s) 192, 204, 265, 631, 751, 804
BstMWI GCNNNNNNNGC 1 cut(s) 515
BstNSI RCATGY 2 cut(s) 868, 1010
BstSCI CCNGG 1 cut(s) 361
BstUI CGCG 1 cut(s) 687
BstV1I GCAGC 1 cut(s) 1001
BstXI CCANNNNNNTGG 1 cut(s) 457
BsuRI GGCC 2 cut(s) 71, 840
BtsCI GGATG 1 cut(s) 158
Cac8I GCNNGC 1 cut(s) 1012
Cfr10I RCCGGY 1 cut(s) 76
CseI GACGC 2 cut(s) 600, 705
Csp6I GTAC 3 cut(s) 305, 410, 481
CspAI ACCGGT 1 cut(s) 76
CviAII CATG 6 cut(s) 595, 824, 851, 865, 995, 1007
CviQI GTAC 3 cut(s) 305, 410, 481
DdeI CTNAG 4 cut(s) 189, 441, 570, 842
DpnI GATC 6 cut(s) 194, 206, 267, 633, 753, 806
DpnII GATC 6 cut(s) 192, 204, 265, 631, 751, 804
DraI TTTAAA 1 cut(s) 525
DriI GACNNNNNGTC 2 cut(s) 495, 547
Eam1104I CTCTTC 1 cut(s) 48
Eam1105I GACNNNNNGTC 2 cut(s) 495, 547
EarI CTCTTC 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 376
EcoRI GAATTC 1 cut(s) 466
EcoT22I ATGCAT 1 cut(s) 511
FaeI CATG 6 cut(s) 598, 827, 854, 868, 998, 1010
FaqI GGGAC 1 cut(s) 344
FatI CATG 6 cut(s) 594, 823, 850, 864, 994, 1006
FblI GTMKAC 1 cut(s) 957
Fnu4HI GCNGC 1 cut(s) 1015
FokI GGATG 1 cut(s) 165
Fsp4HI GCNGC 1 cut(s) 1015
FspBI CTAG 4 cut(s) 557, 692, 1011, 1040
GluI GCNGC 1 cut(s) 1015
GsuI CTGGAG 3 cut(s) 183, 375, 479
HaeIII GGCC 2 cut(s) 71, 840
HapII CCGG 3 cut(s) 77, 272, 363
HgaI GACGC 2 cut(s) 600, 705
Hin1II CATG 6 cut(s) 598, 827, 854, 868, 998, 1010
HincII GTYRAC 1 cut(s) 221
HindII GTYRAC 1 cut(s) 221
HinfI GANTC 6 cut(s) 357, 622, 728, 781, 820, 929
HpaII CCGG 3 cut(s) 77, 272, 363
Hpy166II GTNNAC 5 cut(s) 199, 221, 481, 727, 958
Hpy188I TCNGA 5 cut(s) 51, 261, 571, 756, 953
Hpy188III TCNNGA 7 cut(s) 178, 196, 272, 354, 557, 626, 802
Hpy8I GTNNAC 5 cut(s) 199, 221, 481, 727, 958
HpyAV CCTTC 1 cut(s) 785
HpyCH4III ACNGT 3 cut(s) 744, 911, 1033
HpyCH4IV ACGT 1 cut(s) 173
HpyCH4V TGCA 4 cut(s) 509, 640, 989, 1017
HpyF10VI GCNNNNNNNGC 1 cut(s) 515
HpyF3I CTNAG 4 cut(s) 189, 441, 570, 842
HpySE526I ACGT 1 cut(s) 173
Hsp92II CATG 6 cut(s) 598, 827, 854, 868, 998, 1010
Kpn2I TCCGGA 1 cut(s) 271
Kzo9I GATC 6 cut(s) 192, 204, 265, 631, 751, 804
LpnPI CCDG 8 cut(s) 90, 147, 255, 285, 339, 376, 443, 876
Lsp1109I GCAGC 1 cut(s) 1001
MaeI CTAG 4 cut(s) 557, 692, 1011, 1040
MaeII ACGT 1 cut(s) 173
MaeIII GTNAC 7 cut(s) 26, 227, 539, 653, 867, 999, 1033
MalI GATC 6 cut(s) 194, 206, 267, 633, 753, 806
MbiI CCGCTC 2 cut(s) 6, 920
MboI GATC 6 cut(s) 192, 204, 265, 631, 751, 804
MboII GAAGA 6 cut(s) 35, 38, 260, 304, 475, 776
MluCI AATT 7 cut(s) 37, 118, 127, 246, 466, 530, 983
MlyI GAGTC 3 cut(s) 366, 616, 722
MnlI CCTC 9 cut(s) 372, 399, 437, 481, 510, 565, 694, 850, 891
Mph1103I ATGCAT 1 cut(s) 511
MroI TCCGGA 1 cut(s) 271
MseI TTAA 5 cut(s) 87, 116, 524, 546, 978
MspI CCGG 3 cut(s) 77, 272, 363
MspR9I CCNGG 1 cut(s) 363
MvnI CGCG 1 cut(s) 687
MwoI GCNNNNNNNGC 1 cut(s) 515
NciI CCSGG 1 cut(s) 363
NdeII GATC 6 cut(s) 192, 204, 265, 631, 751, 804
NheI GCTAGC 1 cut(s) 1010
NlaIII CATG 6 cut(s) 598, 827, 854, 868, 998, 1010
NlaIV GGNNCC 1 cut(s) 447
NmuCI GTSAC 3 cut(s) 539, 653, 999
NsiI ATGCAT 1 cut(s) 511
NspI RCATGY 2 cut(s) 868, 1010
NspV TTCGAA 1 cut(s) 784
PaeR7I CTCGAG 1 cut(s) 376
PciI ACATGT 1 cut(s) 864
PcsI WCGNNNNNNNCGW 2 cut(s) 179, 1025
PfeI GAWTC 3 cut(s) 781, 820, 929
PflFI GACNNNGTC 1 cut(s) 692
PinAI ACCGGT 1 cut(s) 76
PkrI GCNGC 1 cut(s) 1016
PleI GAGTC 3 cut(s) 365, 616, 722
PpsI GAGTC 3 cut(s) 365, 616, 722
PscI ACATGT 1 cut(s) 864
PspN4I GGNNCC 1 cut(s) 447
PspXI VCTCGAGB 1 cut(s) 376
PsyI GACNNNGTC 1 cut(s) 692
RsaI GTAC 3 cut(s) 306, 411, 482
RsaNI GTAC 3 cut(s) 305, 410, 481
SaqAI TTAA 5 cut(s) 87, 116, 524, 546, 978
SatI GCNGC 1 cut(s) 1015
Sau3AI GATC 6 cut(s) 192, 204, 265, 631, 751, 804
SchI GAGTC 3 cut(s) 366, 616, 722
ScrFI CCNGG 1 cut(s) 363
SetI ASST 9 cut(s) 145, 176, 310, 447, 693, 726, 837, 946, 1016
Sfr274I CTCGAG 1 cut(s) 376
SfuI TTCGAA 1 cut(s) 784
SlaI CTCGAG 1 cut(s) 376
SmiI ATTTAAAT 1 cut(s) 525
SmlI CTYRAG 1 cut(s) 376
SmoI CTYRAG 1 cut(s) 376
Sse9I AATT 7 cut(s) 37, 118, 127, 246, 466, 530, 983
SsiI CCGC 2 cut(s) 6, 920
SspMI CTAG 4 cut(s) 557, 692, 1011, 1040
StyD4I CCNGG 1 cut(s) 361
SwaI ATTTAAAT 1 cut(s) 525
TaaI ACNGT 3 cut(s) 744, 911, 1033
TaiI ACGT 1 cut(s) 176
TaqI TCGA 4 cut(s) 377, 627, 784, 801
TasI AATT 7 cut(s) 37, 118, 127, 246, 466, 530, 983
TatI WGTACW 2 cut(s) 409, 480
TfiI GAWTC 3 cut(s) 781, 820, 929
Tru1I TTAA 5 cut(s) 87, 116, 524, 546, 978
Tru9I TTAA 5 cut(s) 87, 116, 524, 546, 978
TseFI GTSAC 3 cut(s) 539, 653, 999
TseI GCWGC 1 cut(s) 1014
Tsp45I GTSAC 3 cut(s) 539, 653, 999
TspDTI ATGAA 4 cut(s) 357, 405, 598, 812
Tth111I GACNNNGTC 1 cut(s) 692
XapI RAATTY 3 cut(s) 118, 466, 983
XbaI TCTAGA 1 cut(s) 556
XceI RCATGY 2 cut(s) 868, 1010
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
XhoI CTCGAG 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 957
XspI CTAG 4 cut(s) 557, 692, 1011, 1040
Zsp2I ATGCAT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.