Rroxscaffold_4G00325670

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000342
Physical Location & Seq
Forward (+)
57474770 .. 57476636
1867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325670.1

Sequence Viewer

Length: 804 bp
ATGATCTTCCCCAAAACGACTTTAACAAACTTTTCAAATCATTGCCAAGCTTCTACAAAAAAACTCGAAGAAAGGAAGTGGCTGGAGCCATGCTTCATTGCAGGAATGCCCGGTTCTTTCTATGGCAGGCTCTTTCCTAAGAGTTCTATCAACTTTTTTCACTCTTCTTACGCTCTGCACTGGATCTCTAAGGTTCCAGAAGGTTTGGTAACAAAAGGAGGACTCAACGAGGGAAACATATGCACAGCCAAGACAAGCCCACCTTCTGTGTTTAATGAATACTTTGAGCAATTTAAAAAAGACTTCACAGTGTTTCTAAGGTCTCGTGCAGAAGAGTTGGTCCCTCAGGGTAGAATGGTCCTCACAATCAGGGGGAGAATAAATAGTCATGAACCAGCTTTATTTGAAATTCTTGGATTGAAAATCAATGATATGGTTTTAGAGGGTTTGATTGAAAAGAAAAACCTAGACTACTTCAATATTCCATACTATGAACCTACAATGGAGGAACTGATGGAGCTGATCGAGACCGAAGGATCCTTTAGGTTGCCGGACCATCAAGTGTTTAAACGTGATTGGGACTCTTCTATAAAGGAAGCTGATAGTAGTCTCGGTAAGAAAGCAAGGGCGGGTAAACTTTCCACTCACATTAGGTCTGTGGTGGAGCCTCTTCTGATCAGTCACTTTGGAGAGGGACTCATGGAAGATTTGTTTCACAGGTTTGAAAAAGATGTTCTTGATCACATGGAAAAGGAGAAGTGCCAGTCTATAGATATAGTTCTATCGTTGACAAAGGAGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

267

Amino Acids

30.77

Weight (kDa)

6.17

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 18 - 265 5.9e-82 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14060 AT2G14060 AT3G11480 AT3G21950 AT3G21950 AT5G04370 AT5G04370 AT5G04370 AT5G04380 AT5G04380 AT5G04380 AT5G38020 AT5G38020 AT5G66430
fragaria_vesca FvH4_2g09290 FvH4_2g09290 FvH4_2g09310 FvH4_3g03130
malus_domestica MD10G1071000.v1.1 MD10G1071200.v1.1 MD10G1071500.v1.1 MD10G1218400.v1.1
prunus_persica Prupe.8G092700_v2.0.a1 Prupe.8G092800_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G092900_v2.0.a1 Prupe.8G093000_v2.0.a1 Prupe.8G093100_v2.0.a1 Prupe.8G093200_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093500_v2.0.a1 Prupe.8G093600_v2.0.a1
pyrus_communis pycom10g05660 pycom10g05680 pycom10g18420
rosa_chinensis RchiOBHm_Chr1g0324421 RchiOBHm_Chr1g0324461 RchiOBHm_Chr6g0265311 RchiOBHm_Chr6g0265321 RchiOBHm_Chr6g0265361 RchiOBHm_Chr6g0265391 RchiOBHm_Chr6g0265411
rosa_laevigata RLG00000014186 RLG00000014191 RLG00000014195 RLG00000014197 RLG00000025381 RLG00000030199
rosa_multiflora Rmu_co8237181.1_g000001 Rmu_sc0001780.1_g000008 Rmu_sc0001803.1_g000020 Rmu_sc0002550.1_g000004 Rmu_sc0004082.1_g000012 Rmu_sc0008601.1_g000002 Rmu_sc0011954.1_g000001 Rmu_sc0013000.1_g000001 Rmu_sc0016482.1_g000001 Rmu_sc0018713.1_g000002 Rmu_ssc0000066.1_g000015
rosa_roxburghii Rroxscaffold_159G00432720 Rroxscaffold_159G00432790 Rroxscaffold_159G00432850 Rroxscaffold_175G00432320 Rroxscaffold_175G00432340 Rroxscaffold_175G00432350 Rroxscaffold_175G00432360 Rroxscaffold_2G00105600 Rroxscaffold_2G00105660 Rroxscaffold_4G00325530 Rroxscaffold_4G00325600 Rroxscaffold_4G00325670 Rroxscaffold_6G00424510 Rroxscaffold_7G00202680 Rroxscaffold_7G00202760 Rroxscaffold_7G00202800 Rroxscaffold_7G00202850
rosa_rugosa Rorug01G0045900 Rorug01G0046000 Rorug02G0355000 Rorug02G0355300 Rorug03G0004200 Rorug06G0016700 Rorug06G0016700 Rorug06G0016800 Rorug06G0017100
rosa_samantha Rh1AG061600 Rh1AG061700 Rh1BG052400 Rh1CG063500 Rh1CG063900 Rh1CG064100 Rh2BG415800 Rh3DG067100 Rh6AG138200 Rh6AG138400 Rh6AG138800 Rh6BG137100 Rh6BG137200 Rh6BG137600 Rh6DG121600 Rh6DG121700 Rh6DG121900
rosa_wichuraiana Rw1G005230 Rw1G005470 Rw2G033110 Rw6G011920 Rw6G011940 Rw6G011950 Rw6G011970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 629
AclWI GGATC 3 cut(s) 191, 531, 544
AcsI RAATTY 1 cut(s) 408
AgsI TTSAA 6 cut(s) 36, 407, 421, 455, 478, 725
AluBI AGCT 4 cut(s) 50, 398, 520, 599
AluI AGCT 4 cut(s) 50, 398, 520, 599
Alw26I GTCTC 3 cut(s) 327, 521, 614
AlwI GGATC 3 cut(s) 191, 531, 544
ApoI RAATTY 1 cut(s) 408
AspS9I GGNCC 3 cut(s) 340, 358, 553
AsuC2I CCSGG 1 cut(s) 111
AvaII GGWCC 3 cut(s) 340, 358, 553
AxyI CCTNAGG 1 cut(s) 345
BamHI GGATCC 1 cut(s) 536
BauI CACGAG 1 cut(s) 324
BccI CCATC 2 cut(s) 508, 564
BclI TGATCA 2 cut(s) 675, 739
BcnI CCSGG 1 cut(s) 111
BcoDI GTCTC 3 cut(s) 327, 521, 614
BfaI CTAG 1 cut(s) 467
BfmI CTRYAG 1 cut(s) 768
Bme1390I CCNGG 1 cut(s) 111
Bme18I GGWCC 3 cut(s) 340, 358, 553
BmgT120I GGNCC 3 cut(s) 340, 358, 553
BmiI GGNNCC 5 cut(s) 87, 195, 342, 538, 666
BmrFI CCNGG 1 cut(s) 111
BplI GAGNNNNNCTC 2 cut(s) 681, 713
BpmI CTGGAG 1 cut(s) 104
BpuMI CCSGG 1 cut(s) 111
BsaI GGTCTC 2 cut(s) 327, 521
Bse1I ACTGG 2 cut(s) 185, 763
Bse21I CCTNAGG 1 cut(s) 345
Bse3DI GCAATG 2 cut(s) 40, 96
BseMI GCAATG 2 cut(s) 40, 96
BseMII CTCAG 1 cut(s) 359
BseNI ACTGG 2 cut(s) 185, 763
BsgI GTGCAG 2 cut(s) 161, 348
BsiSI CCGG 2 cut(s) 111, 551
BslFI GGGAC 3 cut(s) 326, 593, 708
BsmAI GTCTC 3 cut(s) 327, 521, 614
BsmFI GGGAC 3 cut(s) 326, 593, 708
BsmI GAATGC 1 cut(s) 111
Bso31I GGTCTC 2 cut(s) 327, 521
Bsp143I GATC 6 cut(s) 3, 183, 522, 536, 675, 739
BspACI CCGC 1 cut(s) 629
BspCNI CTCAG 1 cut(s) 358
BspHI TCATGA 1 cut(s) 388
BspLI GGNNCC 5 cut(s) 87, 195, 342, 538, 666
BspPI GGATC 3 cut(s) 191, 531, 544
BspTNI GGTCTC 2 cut(s) 327, 521
BsrDI GCAATG 2 cut(s) 40, 96
BsrI ACTGG 2 cut(s) 185, 763
BssMI GATC 6 cut(s) 3, 183, 522, 536, 675, 739
BssSI CACGAG 1 cut(s) 324
Bst2BI CACGAG 1 cut(s) 324
Bst4CI ACNGT 1 cut(s) 310
Bst6I CTCTTC 4 cut(s) 169, 327, 589, 675
BstC8I GCNNGC 1 cut(s) 128
BstDEI CTNAG 4 cut(s) 138, 189, 317, 345
BstKTI GATC 6 cut(s) 6, 186, 525, 539, 678, 742
BstMAI GTCTC 3 cut(s) 327, 521, 614
BstMBI GATC 6 cut(s) 3, 183, 522, 536, 675, 739
BstSCI CCNGG 1 cut(s) 109
BstSFI CTRYAG 1 cut(s) 768
BstX2I RGATCY 2 cut(s) 183, 536
BstYI RGATCY 2 cut(s) 183, 536
Bsu36I CCTNAGG 1 cut(s) 345
BtsIMutI CAGTG 2 cut(s) 178, 315
Cac8I GCNNGC 1 cut(s) 128
CciI TCATGA 1 cut(s) 388
Cfr13I GGNCC 3 cut(s) 340, 358, 553
CviAII CATG 4 cut(s) 90, 389, 700, 745
DdeI CTNAG 4 cut(s) 138, 189, 317, 345
DpnI GATC 6 cut(s) 5, 185, 524, 538, 677, 741
DpnII GATC 6 cut(s) 3, 183, 522, 536, 675, 739
DraI TTTAAA 2 cut(s) 295, 568
Eam1104I CTCTTC 4 cut(s) 169, 327, 589, 675
EarI CTCTTC 4 cut(s) 169, 327, 589, 675
Eco31I GGTCTC 2 cut(s) 327, 521
Eco47I GGWCC 3 cut(s) 340, 358, 553
Eco81I CCTNAGG 1 cut(s) 345
FaeI CATG 4 cut(s) 93, 392, 703, 748
FalI AAGNNNNNCTT 4 cut(s) 247, 279, 720, 752
FaqI GGGAC 3 cut(s) 326, 593, 708
FatI CATG 4 cut(s) 89, 388, 699, 744
FauI CCCGC 1 cut(s) 622
FauNDI CATATG 1 cut(s) 239
FbaI TGATCA 2 cut(s) 675, 739
FspBI CTAG 1 cut(s) 467
GsuI CTGGAG 1 cut(s) 104
HapII CCGG 2 cut(s) 111, 551
Hin1II CATG 4 cut(s) 93, 392, 703, 748
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HindIII AAGCTT 1 cut(s) 48
HinfI GANTC 3 cut(s) 222, 581, 696
HpaII CCGG 2 cut(s) 111, 551
Hpy166II GTNNAC 2 cut(s) 635, 789
Hpy188I TCNGA 1 cut(s) 675
Hpy188III TCNNGA 4 cut(s) 197, 389, 526, 737
Hpy8I GTNNAC 2 cut(s) 635, 789
HpyAV CCTTC 3 cut(s) 194, 273, 527
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4IV ACGT 1 cut(s) 571
HpyCH4V TGCA 4 cut(s) 101, 178, 243, 329
HpyF3I CTNAG 4 cut(s) 138, 189, 317, 345
HpySE526I ACGT 1 cut(s) 571
Hsp92II CATG 4 cut(s) 93, 392, 703, 748
Ksp22I TGATCA 2 cut(s) 675, 739
Kzo9I GATC 6 cut(s) 3, 183, 522, 536, 675, 739
LmnI GCTCC 3 cut(s) 85, 517, 664
MaeI CTAG 1 cut(s) 467
MaeII ACGT 1 cut(s) 571
MaeIII GTNAC 2 cut(s) 208, 680
MalI GATC 6 cut(s) 5, 185, 524, 538, 677, 741
MboI GATC 6 cut(s) 3, 183, 522, 536, 675, 739
MboII GAAGA 6 cut(s) 80, 156, 344, 576, 662, 716
MflI RGATCY 2 cut(s) 183, 536
MluCI AATT 2 cut(s) 290, 408
MlyI GAGTC 3 cut(s) 216, 575, 690
MnlI CCTC 8 cut(s) 212, 223, 354, 371, 436, 499, 678, 685
MseI TTAA 5 cut(s) 23, 273, 294, 567, 802
MspI CCGG 2 cut(s) 111, 551
MspR9I CCNGG 1 cut(s) 111
MssI GTTTAAAC 1 cut(s) 568
Mva1269I GAATGC 1 cut(s) 111
NciI CCSGG 1 cut(s) 111
NdeI CATATG 1 cut(s) 239
NdeII GATC 6 cut(s) 3, 183, 522, 536, 675, 739
NlaIII CATG 4 cut(s) 93, 392, 703, 748
NlaIV GGNNCC 5 cut(s) 87, 195, 342, 538, 666
NmuCI GTSAC 1 cut(s) 680
PagI TCATGA 1 cut(s) 388
PctI GAATGC 1 cut(s) 111
PleI GAGTC 3 cut(s) 216, 575, 690
PmeI GTTTAAAC 1 cut(s) 568
PpsI GAGTC 3 cut(s) 216, 575, 690
PspN4I GGNNCC 5 cut(s) 87, 195, 342, 538, 666
PspPI GGNCC 3 cut(s) 340, 358, 553
PsuI RGATCY 2 cut(s) 183, 536
SaqAI TTAA 5 cut(s) 23, 273, 294, 567, 802
Sau3AI GATC 6 cut(s) 3, 183, 522, 536, 675, 739
Sau96I GGNCC 3 cut(s) 340, 358, 553
SchI GAGTC 3 cut(s) 216, 575, 690
ScrFI CCNGG 1 cut(s) 111
SfcI CTRYAG 1 cut(s) 768
SinI GGWCC 3 cut(s) 340, 358, 553
Sse9I AATT 2 cut(s) 290, 408
SsiI CCGC 1 cut(s) 629
SspI AATATT 1 cut(s) 481
SspMI CTAG 1 cut(s) 467
StyD4I CCNGG 1 cut(s) 109
TaaI ACNGT 1 cut(s) 310
TaiI ACGT 1 cut(s) 574
TaqI TCGA 2 cut(s) 66, 525
TaqII GACCGA 1 cut(s) 545
TasI AATT 2 cut(s) 290, 408
Tru1I TTAA 5 cut(s) 23, 273, 294, 567, 802
Tru9I TTAA 5 cut(s) 23, 273, 294, 567, 802
TscAI CASTG 2 cut(s) 185, 315
TseFI GTSAC 1 cut(s) 680
Tsp45I GTSAC 1 cut(s) 680
TspDTI ATGAA 4 cut(s) 85, 291, 405, 507
TspRI CASTG 2 cut(s) 185, 315
VpaK11BI GGWCC 3 cut(s) 340, 358, 553
XapI RAATTY 1 cut(s) 408
XspI CTAG 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.