AT4G08470

Mitogen-Activated Protein Kinase Kinase Kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000243
Physical Location & Seq
Reverse (-)
5383688 .. 5387260
3573 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G08470.2

Sequence Viewer

Length: 1683 bp
ATGGACGTAACTGCTATCTTCGCCGGTGATATTTTGGTGCAATCCAGAGAGTATCTTATCCCTAATGATGTTGTGGATGTTGATGGTGGTATTAAAGCGGTAAGACCACCAATAATTCAGCCCCCTCCGGGAAGAAAACTACCTCTTATTGACTTTCCAGGATCATCGTGGGATTTCCTTACGTATTTTGCTCCAAGTAAAACAGTTAAGCGGCAGAGTTCCTCCTCTTCTGATAATACCTCTGATAAAGAGGAAGTTGAAACGGAAGAGACGCGAGGTATGTTTGTCCAGTTGGGGGATACGGCTCATGAGGCATGCCCATTCGCTACAAACGAGGCTGACTCCTCCAGTACAGTATCCATTATTTCGCCGAGTTATGCCTCTAGAGGTTCTATCGTCCCTTCTTGGCTGAAGAGGAAATTTCTGGGACGAGTATCATTAGGTTTTGTGTATGAAGGCAGTTCAGGTTCCTCCGTTGGTTCCGAGTCGACTTGTTCCTTGATGACGCCTTCTCTGGAGTTCCCGGATCGCATCAGTTTCCGGAAAAAGGATTTCAGTGAAAAAGGTCCTAGTCGACACGTTTGGGAAAAGCGTAAATTGACGCGTGCCAAGTTGATTGAAAATTTTTGCAATCCGGAGGACATTGAACCAGTCACATCTTGGCTGAAGGGTCAACTTCTAGGAGAAGAATCATTTGCCTCTGTGTATGAAGCCATTTCAGATTCCTCTGTTGGTTCCGAGTCGACTTGTTCCTTGATGACGCCTTCTATGGAGTTCCCGGATCGCATCAGTTTCCGAAAAAGGGATTTCAGCGAAGAAGGTCCTAGTGGACGTGTTAAGGAAAAGCGTAAATTGATGCGTAACAAGTTGATTGAGAATTTTCGTAAGCCTGAGGATATCACGTCTTGGCTGAAGGGTCAACTTCTAGGACGAGGATCATATGCCTCTGTGTATGAAGCCATTTCAGAAGATGGGGACTTCTTTGCTGTCAAGGAAGTTTCACTTCTTGATAAGGGAATTCAGGCACAAGAATGCATTCAACAACTTGAGGGGGAGATTGCACTACTTAGTCAGCTTCAGCATCAGAATATTGTGCGATATCGTGGCACAGCCAAGGACGTGTCAAAATTGTACATCTTTCTTGAGCTTGTAACCCAAGGATCCGTTCAAAAACTCTACGAAAGATACCAACTTAGCTACACAGTAGTCTCCTTGTATACAAGACAAATTCTTGCTGGGTTGAATTATCTCCACGATAAAGGCTTTGTTCACAGGGACATTAAATGTGCAAATATGTTGGTGGACGCTAATGGCACCGTCAAACTTGCAGATTTTGGATTGGCAGAGGCGTCAAAGTTCAACGACATTATGTCTTGCAAGGGAACATTATTTTGGATGGCTCCGGAGGTTATTAACCGAAAGGATAGTGATGGCAATGGAAGTCCAGCTGATATATGGAGCCTTGGGTGCACTGTGCTGGAAATGTGTACTGGTCAGATCCCCTACTCCGATCTAAAACCCATTCAAGCCGCGTTTAAGATTGGAAGGGGTACGCTTCCAGACGTACCTGATACTTTATCACTAGACGCTCGGCATTTCATACTTACGTGTCTCAAAGTGAACCCGGAAGAGCGGCCAACTGCAGCTGAACTGCTGCACCATCCATTTGTTATAAATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

560

Amino Acids

62.31

Weight (kDa)

5.62

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 305 - 471 3.8e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 307 - 477 9.5e-30 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1673
AccB1I GGYRCC 1 cut(s) 1313
AccBSI CCGCTC 1 cut(s) 1633
AccI GTMKAC 4 cut(s) 488, 574, 743, 1217
AccII CGCG 3 cut(s) 274, 604, 1532
AccIII TCCGGA 3 cut(s) 540, 634, 1402
AciI CCGC 4 cut(s) 98, 211, 1530, 1633
AclWI GGATC 7 cut(s) 169, 534, 789, 943, 1155, 1168, 1492
AcoI YGGCCR 1 cut(s) 1634
AcsI RAATTY 5 cut(s) 419, 622, 877, 1017, 1227
AcuI CTGAAG 4 cut(s) 431, 686, 932, 1061
AcyI GRCGYC 3 cut(s) 506, 761, 1349
AfaI GTAC 5 cut(s) 352, 1133, 1489, 1552, 1566
AfiI CCNNNNNNNGG 4 cut(s) 128, 295, 547, 802
AflIII ACRYGT 5 cut(s) 577, 602, 832, 1119, 1607
AgsI TTSAA 8 cut(s) 260, 620, 647, 1040, 1169, 1243, 1360, 1526
AhdI GACNNNNNGTC 1 cut(s) 1369
AjiI CACGTC 3 cut(s) 833, 903, 1120
AjnI CCWGG 1 cut(s) 157
AjuI GAANNNNNNNTTGG 4 cut(s) 1149, 1181, 1375, 1407
AluBI AGCT 5 cut(s) 1075, 1147, 1197, 1448, 1646
AluI AGCT 5 cut(s) 1075, 1147, 1197, 1448, 1646
Alw21I GWGCWC 1 cut(s) 1472
Alw26I GTCTC 3 cut(s) 263, 1213, 1616
Alw44I GTGCAC 1 cut(s) 1468
AlwI GGATC 7 cut(s) 169, 534, 789, 943, 1155, 1168, 1492
Aor13HI TCCGGA 3 cut(s) 540, 634, 1402
AoxI GGCC 1 cut(s) 1634
ApaLI GTGCAC 1 cut(s) 1468
ApeKI GCWGC 2 cut(s) 1643, 1654
ApoI RAATTY 5 cut(s) 419, 622, 877, 1017, 1227
Asp700I GAANNNNTTC 1 cut(s) 1035
AspS9I GGNCC 2 cut(s) 566, 821
AsuC2I CCSGG 4 cut(s) 129, 524, 779, 1625
AsuHPI GGTGA 1 cut(s) 38
AvaII GGWCC 2 cut(s) 566, 821
AxyI CCTNAGG 1 cut(s) 891
BaeGI GKGCMC 1 cut(s) 1472
BamHI GGATCC 1 cut(s) 1160
BanI GGYRCC 1 cut(s) 1313
Bbv12I GWGCWC 1 cut(s) 1472
BbvI GCAGC 2 cut(s) 1641, 1655
BccI CCATC 5 cut(s) 77, 965, 1390, 1424, 1668
BceAI ACGGC 1 cut(s) 318
BciT130I CCWGG 1 cut(s) 159
BciVI GTATCC 2 cut(s) 292, 367
BcnI CCSGG 4 cut(s) 129, 524, 779, 1625
BcoDI GTCTC 3 cut(s) 263, 1213, 1616
BfaI CTAG 6 cut(s) 384, 570, 680, 825, 926, 1583
BfmI CTRYAG 1 cut(s) 1641
BfuI GTATCC 2 cut(s) 292, 367
BisI GCNGC 5 cut(s) 212, 1530, 1634, 1644, 1655
BlsI GCNGC 5 cut(s) 213, 1531, 1635, 1645, 1656
Bme1390I CCNGG 5 cut(s) 129, 159, 524, 779, 1625
Bme18I GGWCC 2 cut(s) 566, 821
BmeRI GACNNNNNGTC 1 cut(s) 1369
BmgBI CACGTC 3 cut(s) 833, 903, 1120
BmgT120I GGNCC 2 cut(s) 566, 821
BmiI GGNNCC 7 cut(s) 469, 481, 736, 1162, 1315, 1401, 1460
BmrFI CCNGG 5 cut(s) 129, 159, 524, 779, 1625
BmsI GCATC 4 cut(s) 540, 795, 846, 1090
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 2 cut(s) 331, 536
BpuEI CTTGAG 2 cut(s) 1067, 1163
BpuMI CCSGG 4 cut(s) 129, 524, 779, 1625
BsaAI YACGTR 2 cut(s) 183, 1608
BsaHI GRCGYC 3 cut(s) 506, 761, 1349
BsaJI CCNNGG 3 cut(s) 1113, 1156, 1462
BsaWI WCCGGW 3 cut(s) 540, 634, 1402
Bsc4I CCNNNNNNNGG 4 cut(s) 128, 295, 547, 802
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 4 cut(s) 289, 348, 650, 1495
Bse21I CCTNAGG 1 cut(s) 891
Bse3DI GCAATG 1 cut(s) 1441
BseAI TCCGGA 3 cut(s) 540, 634, 1402
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 3 cut(s) 1113, 1156, 1462
BseGI GGATG 3 cut(s) 82, 1401, 1660
BseLI CCNNNNNNNGG 4 cut(s) 128, 295, 547, 802
BseMI GCAATG 1 cut(s) 1441
BseMII CTCAG 1 cut(s) 882
BseNI ACTGG 4 cut(s) 289, 348, 650, 1495
BseRI GAGGAG 2 cut(s) 214, 334
BseSI GKGCMC 1 cut(s) 1472
BseXI GCAGC 2 cut(s) 1641, 1655
BseYI CCCAGC 1 cut(s) 1235
BsgI GTGCAG 1 cut(s) 1640
Bsh1236I CGCG 3 cut(s) 274, 604, 1532
BshFI GGCC 1 cut(s) 1636
BshNI GGYRCC 1 cut(s) 1313
BsiHKAI GWGCWC 1 cut(s) 1472
BsiSI CCGG 8 cut(s) 24, 128, 524, 541, 635, 779, 1403, 1625
BslFI GGGAC 4 cut(s) 383, 441, 989, 1289
BslI CCNNNNNNNGG 4 cut(s) 128, 295, 547, 802
BsmAI GTCTC 3 cut(s) 263, 1213, 1616
BsmBI CGTCTC 1 cut(s) 263
BsmFI GGGAC 4 cut(s) 383, 441, 989, 1289
BsmI GAATGC 2 cut(s) 1035, 1037
BsnI GGCC 1 cut(s) 1636
Bsp1286I GDGCHC 1 cut(s) 1472
Bsp13I TCCGGA 3 cut(s) 540, 634, 1402
Bsp1407I TGTACA 1 cut(s) 1131
Bsp143I GATC 7 cut(s) 161, 526, 781, 935, 1160, 1497, 1510
BspACI CCGC 4 cut(s) 98, 211, 1530, 1633
BspANI GGCC 1 cut(s) 1636
BspCNI CTCAG 1 cut(s) 883
BspEI TCCGGA 3 cut(s) 540, 634, 1402
BspFNI CGCG 3 cut(s) 274, 604, 1532
BspHI TCATGA 1 cut(s) 307
BspLI GGNNCC 7 cut(s) 469, 481, 736, 1162, 1315, 1401, 1460
BspMAI CTGCAG 1 cut(s) 1645
BspPI GGATC 7 cut(s) 169, 534, 789, 943, 1155, 1168, 1492
BspQI GCTCTTC 1 cut(s) 1623
BspT107I GGYRCC 1 cut(s) 1313
BsrBI CCGCTC 1 cut(s) 1633
BsrDI GCAATG 1 cut(s) 1441
BsrFI RCCGGY 1 cut(s) 23
BsrGI TGTACA 1 cut(s) 1131
BsrI ACTGG 4 cut(s) 289, 348, 650, 1495
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 3 cut(s) 1113, 1156, 1462
BssMI GATC 7 cut(s) 161, 526, 781, 935, 1160, 1497, 1510
BssNAI GTATAC 1 cut(s) 1218
BssNI GRCGYC 3 cut(s) 506, 761, 1349
BssT1I CCWWGG 3 cut(s) 1113, 1156, 1462
Bst1107I GTATAC 1 cut(s) 1218
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 5 cut(s) 205, 355, 1204, 1318, 1474
Bst6I CTCTTC 4 cut(s) 232, 261, 407, 1623
BstACI GRCGYC 3 cut(s) 506, 761, 1349
BstAUI TGTACA 1 cut(s) 1131
BstBAI YACGTR 2 cut(s) 183, 1608
BstC8I GCNNGC 2 cut(s) 316, 606
BstDEI CTNAG 3 cut(s) 891, 1067, 1193
BstF5I GGATG 3 cut(s) 82, 1401, 1660
BstFNI CGCG 3 cut(s) 274, 604, 1532
BstKTI GATC 7 cut(s) 164, 529, 784, 938, 1163, 1500, 1513
BstMAI GTCTC 3 cut(s) 263, 1213, 1616
BstMBI GATC 7 cut(s) 161, 526, 781, 935, 1160, 1497, 1510
BstMWI GCNNNNNNNGC 3 cut(s) 20, 311, 1467
BstNI CCWGG 1 cut(s) 159
BstNSI RCATGY 1 cut(s) 318
BstSCI CCNGG 5 cut(s) 127, 157, 522, 777, 1623
BstSFI CTRYAG 1 cut(s) 1641
BstSLI GKGCMC 1 cut(s) 1472
BstSNI TACGTA 1 cut(s) 183
BstUI CGCG 3 cut(s) 274, 604, 1532
BstV1I GCAGC 2 cut(s) 1641, 1655
BstX2I RGATCY 2 cut(s) 1160, 1497
BstYI RGATCY 2 cut(s) 1160, 1497
BstZ17I GTATAC 1 cut(s) 1218
Bsu36I CCTNAGG 1 cut(s) 891
BsuI GTATCC 2 cut(s) 292, 367
BsuRI GGCC 1 cut(s) 1636
BtrI CACGTC 3 cut(s) 833, 903, 1120
BtsCI GGATG 3 cut(s) 82, 1401, 1660
BtsIMutI CAGTG 2 cut(s) 562, 1470
Cac8I GCNNGC 2 cut(s) 316, 606
CciI TCATGA 1 cut(s) 307
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 2 cut(s) 566, 821
CseI GACGC 7 cut(s) 280, 514, 610, 769, 1313, 1338, 1595
Csp6I GTAC 5 cut(s) 351, 1132, 1488, 1551, 1565
CviAII CATG 2 cut(s) 308, 315
CviQI GTAC 5 cut(s) 351, 1132, 1488, 1551, 1565
DdeI CTNAG 3 cut(s) 891, 1067, 1193
DpnI GATC 7 cut(s) 163, 528, 783, 937, 1162, 1499, 1512
DpnII GATC 7 cut(s) 161, 526, 781, 935, 1160, 1497, 1510
DriI GACNNNNNGTC 1 cut(s) 1369
EaeI YGGCCR 1 cut(s) 1634
Eam1104I CTCTTC 4 cut(s) 232, 261, 407, 1623
Eam1105I GACNNNNNGTC 1 cut(s) 1369
EarI CTCTTC 4 cut(s) 232, 261, 407, 1623
Eco105I TACGTA 1 cut(s) 183
Eco130I CCWWGG 3 cut(s) 1113, 1156, 1462
Eco32I GATATC 2 cut(s) 898, 1100
Eco47I GGWCC 2 cut(s) 566, 821
Eco57I CTGAAG 4 cut(s) 431, 686, 932, 1061
Eco81I CCTNAGG 1 cut(s) 891
EcoO109I RGGNCCY 2 cut(s) 566, 821
EcoRI GAATTC 1 cut(s) 1017
EcoRII CCWGG 1 cut(s) 157
EcoRV GATATC 2 cut(s) 898, 1100
EcoT14I CCWWGG 3 cut(s) 1113, 1156, 1462
EcoT22I ATGCAT 1 cut(s) 1037
ErhI CCWWGG 3 cut(s) 1113, 1156, 1462
Esp3I CGTCTC 1 cut(s) 263
FaeI CATG 2 cut(s) 311, 318
FalI AAGNNNNNCTT 2 cut(s) 987, 1019
FaqI GGGAC 4 cut(s) 383, 441, 989, 1289
FatI CATG 2 cut(s) 307, 314
FauNDI CATATG 1 cut(s) 940
FblI GTMKAC 4 cut(s) 488, 574, 743, 1217
Fnu4HI GCNGC 5 cut(s) 212, 1530, 1634, 1644, 1655
FokI GGATG 3 cut(s) 89, 1408, 1647
Fsp4HI GCNGC 5 cut(s) 212, 1530, 1634, 1644, 1655
FspBI CTAG 6 cut(s) 384, 570, 680, 825, 926, 1583
GluI GCNGC 5 cut(s) 212, 1530, 1634, 1644, 1655
GsaI CCCAGC 1 cut(s) 1239
GsuI CTGGAG 2 cut(s) 331, 536
HaeIII GGCC 1 cut(s) 1636
HapII CCGG 8 cut(s) 24, 128, 524, 541, 635, 779, 1403, 1625
HgaI GACGC 7 cut(s) 280, 514, 610, 769, 1313, 1338, 1595
Hin1I GRCGYC 3 cut(s) 506, 761, 1349
Hin1II CATG 2 cut(s) 311, 318
HincII GTYRAC 5 cut(s) 489, 575, 674, 744, 920
HindII GTYRAC 5 cut(s) 489, 575, 674, 744, 920
HinfI GANTC 5 cut(s) 341, 485, 689, 722, 740
HpaII CCGG 8 cut(s) 24, 128, 524, 541, 635, 779, 1403, 1625
HphI GGTGA 1 cut(s) 38
HpyAV CCTTC 8 cut(s) 411, 449, 519, 661, 774, 812, 907, 1539
HpyCH4III ACNGT 5 cut(s) 205, 355, 1204, 1318, 1474
HpyCH4IV ACGT 8 cut(s) 6, 182, 579, 832, 902, 1119, 1563, 1607
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 311, 1467
HpyF3I CTNAG 3 cut(s) 891, 1067, 1193
HpySE526I ACGT 8 cut(s) 6, 182, 579, 832, 902, 1119, 1563, 1607
Hsp92I GRCGYC 3 cut(s) 506, 761, 1349
Hsp92II CATG 2 cut(s) 311, 318
Kpn2I TCCGGA 3 cut(s) 540, 634, 1402
Kzo9I GATC 7 cut(s) 161, 526, 781, 935, 1160, 1497, 1510
LguI GCTCTTC 1 cut(s) 1623
LmnI GCTCC 3 cut(s) 196, 1405, 1458
Lsp1109I GCAGC 2 cut(s) 1641, 1655
LweI GCATC 4 cut(s) 540, 795, 846, 1090
MaeI CTAG 6 cut(s) 384, 570, 680, 825, 926, 1583
MaeII ACGT 8 cut(s) 6, 182, 579, 832, 902, 1119, 1563, 1607
MaeIII GTNAC 4 cut(s) 7, 652, 860, 1150
MalI GATC 7 cut(s) 163, 528, 783, 937, 1162, 1499, 1512
MbiI CCGCTC 1 cut(s) 1633
MboI GATC 7 cut(s) 161, 526, 781, 935, 1160, 1497, 1510
MboII GAAGA 9 cut(s) 10, 144, 219, 278, 424, 698, 827, 980, 1640
MflI RGATCY 2 cut(s) 1160, 1497
MhlI GDGCHC 1 cut(s) 1472
MluI ACGCGT 1 cut(s) 602
MlyI GAGTC 3 cut(s) 335, 494, 749
Mph1103I ATGCAT 1 cut(s) 1037
MroI TCCGGA 3 cut(s) 540, 634, 1402
MroXI GAANNNNTTC 1 cut(s) 1035
MseI TTAA 6 cut(s) 93, 207, 837, 1281, 1413, 1536
MslI CAYNNNNRTG 1 cut(s) 1030
MspA1I CMGCKG 2 cut(s) 1448, 1646
MspI CCGG 8 cut(s) 24, 128, 524, 541, 635, 779, 1403, 1625
MspR9I CCNGG 5 cut(s) 129, 159, 524, 779, 1625
Mva1269I GAATGC 2 cut(s) 1035, 1037
MvaI CCWGG 1 cut(s) 159
MvnI CGCG 3 cut(s) 274, 604, 1532
MwoI GCNNNNNNNGC 3 cut(s) 20, 311, 1467
NciI CCSGG 4 cut(s) 129, 524, 779, 1625
NdeI CATATG 1 cut(s) 940
NdeII GATC 7 cut(s) 161, 526, 781, 935, 1160, 1497, 1510
NlaIII CATG 2 cut(s) 311, 318
NlaIV GGNNCC 7 cut(s) 469, 481, 736, 1162, 1315, 1401, 1460
NmeAIII GCCGAG 2 cut(s) 396, 1570
NmuCI GTSAC 1 cut(s) 652
NsiI ATGCAT 1 cut(s) 1037
NspI RCATGY 1 cut(s) 318
PaeI GCATGC 1 cut(s) 318
PagI TCATGA 1 cut(s) 307
PciSI GCTCTTC 1 cut(s) 1623
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PctI GAATGC 2 cut(s) 1035, 1037
PdmI GAANNNNTTC 1 cut(s) 1035
PfeI GAWTC 2 cut(s) 689, 722
PfoI TCCNGGA 4 cut(s) 127, 157, 522, 777
PkrI GCNGC 5 cut(s) 213, 1531, 1635, 1645, 1656
PleI GAGTC 3 cut(s) 335, 493, 748
PpsI GAGTC 3 cut(s) 335, 493, 748
Ppu21I YACGTR 2 cut(s) 183, 1608
PpuMI RGGWCCY 2 cut(s) 566, 821
PsiI TTATAA 1 cut(s) 1673
Psp5II RGGWCCY 2 cut(s) 566, 821
Psp6I CCWGG 1 cut(s) 157
PspFI CCCAGC 1 cut(s) 1235
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 7 cut(s) 469, 481, 736, 1162, 1315, 1401, 1460
PspPI GGNCC 2 cut(s) 566, 821
PspPPI RGGWCCY 2 cut(s) 566, 821
PstI CTGCAG 1 cut(s) 1645
PsuI RGATCY 2 cut(s) 1160, 1497
PvuII CAGCTG 2 cut(s) 1448, 1646
RsaI GTAC 5 cut(s) 352, 1133, 1489, 1552, 1566
RsaNI GTAC 5 cut(s) 351, 1132, 1488, 1551, 1565
RseI CAYNNNNRTG 1 cut(s) 1030
SalI GTCGAC 3 cut(s) 487, 573, 742
SapI GCTCTTC 1 cut(s) 1623
SaqAI TTAA 6 cut(s) 93, 207, 837, 1281, 1413, 1536
SatI GCNGC 5 cut(s) 212, 1530, 1634, 1644, 1655
Sau3AI GATC 7 cut(s) 161, 526, 781, 935, 1160, 1497, 1510
Sau96I GGNCC 2 cut(s) 566, 821
SchI GAGTC 3 cut(s) 335, 494, 749
ScrFI CCNGG 5 cut(s) 129, 159, 524, 779, 1625
SduI GDGCHC 1 cut(s) 1472
SfaNI GCATC 4 cut(s) 540, 795, 846, 1090
SfcI CTRYAG 1 cut(s) 1641
SgrAI CRCCGGYG 1 cut(s) 23
SinI GGWCC 2 cut(s) 566, 821
SmiMI CAYNNNNRTG 1 cut(s) 1030
SmlI CTYRAG 2 cut(s) 1046, 1142
SmoI CTYRAG 2 cut(s) 1046, 1142
SnaBI TACGTA 1 cut(s) 183
SphI GCATGC 1 cut(s) 318
SsiI CCGC 4 cut(s) 98, 211, 1530, 1633
SspI AATATT 1 cut(s) 1090
SspMI CTAG 6 cut(s) 384, 570, 680, 825, 926, 1583
StyD4I CCNGG 5 cut(s) 127, 157, 522, 777, 1623
StyI CCWWGG 3 cut(s) 1113, 1156, 1462
TaaI ACNGT 5 cut(s) 205, 355, 1204, 1318, 1474
TaiI ACGT 8 cut(s) 9, 185, 582, 835, 905, 1122, 1566, 1610
TaqI TCGA 3 cut(s) 488, 574, 743
TatI WGTACW 3 cut(s) 350, 1131, 1487
TauI GCSGC 3 cut(s) 214, 1532, 1636
TfiI GAWTC 2 cut(s) 689, 722
Tru1I TTAA 6 cut(s) 93, 207, 837, 1281, 1413, 1536
Tru9I TTAA 6 cut(s) 93, 207, 837, 1281, 1413, 1536
TscAI CASTG 2 cut(s) 562, 1477
TseFI GTSAC 1 cut(s) 652
TseI GCWGC 2 cut(s) 1643, 1654
Tsp45I GTSAC 1 cut(s) 652
TspDTI ATGAA 4 cut(s) 468, 723, 969, 1588
TspGWI ACGGA 3 cut(s) 278, 463, 1153
TspRI CASTG 2 cut(s) 562, 1477
VneI GTGCAC 1 cut(s) 1468
VpaK11BI GGWCC 2 cut(s) 566, 821
XapI RAATTY 5 cut(s) 419, 622, 877, 1017, 1227
XbaI TCTAGA 1 cut(s) 383
XceI RCATGY 1 cut(s) 318
XcmI CCANNNNNNNNNTGG 3 cut(s) 165, 657, 1452
XmiI GTMKAC 4 cut(s) 488, 574, 743, 1217
XmnI GAANNNNTTC 1 cut(s) 1035
XspI CTAG 6 cut(s) 384, 570, 680, 825, 926, 1583
Zsp2I ATGCAT 1 cut(s) 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.