RchiOBHm_Chr5g0080301

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000243
Physical Location & Seq
Reverse (-)
86223280 .. 86228911
5632 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35462

Sequence Viewer

Length: 1017 bp
ATGCTTTCGCCAAGCATGCAAGGCGATACAAATGCTTTAACAAAAGTGGCCAATTTGTCAGGGTGGGATACGCGGGATTACGACACCGACAAAGATCTTGTCGAAGACATTGTGGAAAATGTGTACAGTAAACTACAATCTGTGGTGGTATCCCAGAGCCCTGACTCGACAAAAATTGAAGGCACATTGGCGGGGCCGAGCGGGTGTTGTTCTGGGAAGCCTAAGCTTACTGTCACTAATTGGAAAAAGGGTGATCTTCTCGGCAGCGGCTCTTTCGGGTCTGTGTATGAAGGAATTACTGATTGTGGACGCTTCATTGCTGTCAAGGAAGTTCCCTTGCTTGATCAAAGAAGAGTATCTCAACTTGAACAGGAGATTGCTCTTCTGAGCCAGTTTGAACATGAGAACATACTCTATATCTTGCTGGAACTTGTAACCGAAGGCTCCCTTCGAAAGCTATATCAAACATACCCTCTTACAGATTCGCATGTCTCTGTATACACTAGACAGATCTTGCAAGGTCTGAAGTATCTTCATGACCAAAATGTGATTCACAGGGACATTAAATGCGCAAATATTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCGGACTTTGGATTGGCAAAGGCTATCAAAACGAACGACATGTTATCTTGCCAAGGAACTGCAAACTGGATGGCCCCTGAGGTTGTTAACCCTGAAAGTCCAGGTTATGGCCTTCCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTAGGGAGGTTCCATACCCTAATCTTGAATGGATGGCGGCAGCATGTAAAATTATGATGGGGAAGCTCCCTCTGGTTCCTGATTCTCTTTCAAGAGAGGCACAAGATTTTATCCATCGATGCTTACAAGTTAAGCCAGATAATCGTCCCACTGCTGCTCAGCTTTTAGAACATCCGTTTGTAAATAAGCCCCTTCCTCCCACGTCTTCTGGATCAGTATCTCCTTACAATCACCACAGGCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

338

Amino Acids

37.33

Weight (kDa)

5.73

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 82 - 317 2.8e-60 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 84 - 313 6.4e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 571
AccB7I CCANNNNNTGG 1 cut(s) 716
AccBSI CCGCTC 1 cut(s) 201
AccI GTMKAC 1 cut(s) 498
AccII CGCG 1 cut(s) 73
AciI CCGC 6 cut(s) 73, 191, 201, 267, 611, 806
AclWI GGATC 2 cut(s) 604, 988
AcoI YGGCCR 1 cut(s) 48
AcuI CTGAAG 1 cut(s) 545
AfaI GTAC 2 cut(s) 125, 751
AfiI CCNNNNNNNGG 1 cut(s) 716
AflIII ACRYGT 1 cut(s) 648
AgsI TTSAA 5 cut(s) 179, 368, 398, 797, 861
AjiI CACGTC 1 cut(s) 972
AjnI CCWGG 1 cut(s) 709
AjuI GAANNNNNNNTTGG 2 cut(s) 534, 566
AluBI AGCT 6 cut(s) 226, 457, 607, 728, 835, 931
AluI AGCT 6 cut(s) 226, 457, 607, 728, 835, 931
Alw26I GTCTC 1 cut(s) 496
AlwI GGATC 2 cut(s) 604, 988
AoxI GGCC 4 cut(s) 48, 194, 681, 718
ApeKI GCWGC 3 cut(s) 264, 809, 923
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
AspLEI GCGC 1 cut(s) 572
AspS9I GGNCC 2 cut(s) 194, 682
AsuHPI GGTGA 2 cut(s) 263, 992
AsuII TTCGAA 1 cut(s) 451
AxyI CCTNAGG 1 cut(s) 687
BalI TGGCCA 1 cut(s) 50
BanII GRGCYC 1 cut(s) 161
BbsI GAAGAC 2 cut(s) 111, 966
BbvI GCAGC 3 cut(s) 276, 821, 910
BccI CCATC 4 cut(s) 673, 796, 820, 891
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BciT130I CCWGG 1 cut(s) 711
BciVI GTATCC 2 cut(s) 61, 160
BclI TGATCA 1 cut(s) 343
BcoDI GTCTC 1 cut(s) 496
BfaI CTAG 2 cut(s) 504, 771
BfuI GTATCC 2 cut(s) 61, 160
BglII AGATCT 2 cut(s) 94, 510
BisI GCNGC 5 cut(s) 265, 268, 807, 810, 924
BlpI GCTNAGC 1 cut(s) 927
BlsI GCNGC 5 cut(s) 266, 269, 808, 811, 925
Bme1390I CCNGG 1 cut(s) 711
BmgBI CACGTC 1 cut(s) 972
BmgT120I GGNCC 2 cut(s) 194, 682
BmiI GGNNCC 5 cut(s) 195, 445, 684, 780, 846
BmrFI CCNGG 1 cut(s) 711
BmsI GCATC 1 cut(s) 878
BpiI GAAGAC 2 cut(s) 111, 966
Bpu10I CCTNAGC 1 cut(s) 222
Bpu1102I GCTNAGC 1 cut(s) 927
Bpu14I TTCGAA 1 cut(s) 451
Bsa29I ATCGAT 1 cut(s) 886
BsaBI GATNNNNATC 1 cut(s) 985
BsaJI CCNNGG 1 cut(s) 661
Bsc4I CCNNNNNNNGG 1 cut(s) 716
Bse1I ACTGG 2 cut(s) 391, 680
Bse21I CCTNAGG 1 cut(s) 687
Bse3DI GCAATG 1 cut(s) 315
Bse8I GATNNNNATC 1 cut(s) 985
BseBI CCWGG 1 cut(s) 711
BseCI ATCGAT 1 cut(s) 886
BseDI CCNNGG 1 cut(s) 661
BseGI GGATG 4 cut(s) 684, 752, 807, 940
BseJI GATNNNNATC 1 cut(s) 985
BseLI CCNNNNNNNGG 1 cut(s) 716
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 3 cut(s) 377, 678, 941
BseNI ACTGG 2 cut(s) 391, 680
BseXI GCAGC 3 cut(s) 276, 821, 910
Bsh1236I CGCG 1 cut(s) 73
BshFI GGCC 4 cut(s) 50, 196, 683, 720
BshVI ATCGAT 1 cut(s) 886
BslFI GGGAC 2 cut(s) 572, 900
BslI CCNNNNNNNGG 1 cut(s) 716
BsmAI GTCTC 1 cut(s) 496
BsmFI GGGAC 2 cut(s) 572, 900
BsnI GGCC 4 cut(s) 50, 196, 683, 720
Bsp119I TTCGAA 1 cut(s) 451
Bsp1286I GDGCHC 1 cut(s) 161
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 6 cut(s) 94, 253, 343, 510, 596, 980
Bsp1720I GCTNAGC 1 cut(s) 927
BspACI CCGC 6 cut(s) 73, 191, 201, 267, 611, 806
BspANI GGCC 4 cut(s) 50, 196, 683, 720
BspCNI CTCAG 3 cut(s) 378, 679, 940
BspDI ATCGAT 1 cut(s) 886
BspFNI CGCG 1 cut(s) 73
BspHI TCATGA 1 cut(s) 535
BspLI GGNNCC 5 cut(s) 195, 445, 684, 780, 846
BspPI GGATC 2 cut(s) 604, 988
BspQI GCTCTTC 1 cut(s) 387
BspT104I TTCGAA 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 201
BsrDI GCAATG 1 cut(s) 315
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 2 cut(s) 391, 680
BssECI CCNNGG 1 cut(s) 661
BssMI GATC 6 cut(s) 94, 253, 343, 510, 596, 980
BssNAI GTATAC 1 cut(s) 499
BssT1I CCWWGG 1 cut(s) 661
Bst1107I GTATAC 1 cut(s) 499
Bst2UI CCWGG 1 cut(s) 711
Bst4CI ACNGT 3 cut(s) 128, 232, 754
Bst6I CTCTTC 2 cut(s) 346, 387
BstAUI TGTACA 1 cut(s) 123
BstBI TTCGAA 1 cut(s) 451
BstC8I GCNNGC 3 cut(s) 17, 588, 609
BstDEI CTNAG 4 cut(s) 222, 386, 687, 927
BstF5I GGATG 4 cut(s) 684, 752, 807, 940
BstFNI CGCG 1 cut(s) 73
BstHHI GCGC 1 cut(s) 572
BstKTI GATC 6 cut(s) 97, 256, 346, 513, 599, 983
BstMAI GTCTC 1 cut(s) 496
BstMBI GATC 6 cut(s) 94, 253, 343, 510, 596, 980
BstMWI GCNNNNNNNGC 2 cut(s) 16, 21
BstNI CCWGG 1 cut(s) 711
BstNSI RCATGY 5 cut(s) 19, 491, 590, 652, 816
BstSCI CCNGG 1 cut(s) 709
BstUI CGCG 1 cut(s) 73
BstV1I GCAGC 3 cut(s) 276, 821, 910
BstV2I GAAGAC 2 cut(s) 111, 966
BstX2I RGATCY 3 cut(s) 94, 510, 596
BstYI RGATCY 3 cut(s) 94, 510, 596
BstZ17I GTATAC 1 cut(s) 499
Bsu15I ATCGAT 1 cut(s) 886
Bsu36I CCTNAGG 1 cut(s) 687
BsuI GTATCC 2 cut(s) 61, 160
BsuRI GGCC 4 cut(s) 50, 196, 683, 720
BsuTUI ATCGAT 1 cut(s) 886
BtrI CACGTC 1 cut(s) 972
BtsCI GGATG 4 cut(s) 684, 752, 807, 940
BtsI GCAGTG 1 cut(s) 918
BtsIMutI CAGTG 1 cut(s) 918
Cac8I GCNNGC 3 cut(s) 17, 588, 609
CciI TCATGA 1 cut(s) 535
CfoI GCGC 1 cut(s) 572
Cfr13I GGNCC 2 cut(s) 194, 682
ClaI ATCGAT 1 cut(s) 886
CseI GACGC 1 cut(s) 318
Csp6I GTAC 2 cut(s) 124, 750
CviAII CATG 7 cut(s) 16, 401, 488, 536, 587, 649, 813
CviQI GTAC 2 cut(s) 124, 750
DdeI CTNAG 4 cut(s) 222, 386, 687, 927
DpnI GATC 6 cut(s) 96, 255, 345, 512, 598, 982
DpnII GATC 6 cut(s) 94, 253, 343, 510, 596, 980
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 2 cut(s) 346, 387
EarI CTCTTC 2 cut(s) 346, 387
Eco130I CCWWGG 1 cut(s) 661
Eco24I GRGCYC 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 545
Eco81I CCTNAGG 1 cut(s) 687
EcoRII CCWGG 1 cut(s) 709
EcoT14I CCWWGG 1 cut(s) 661
EcoT38I GRGCYC 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 661
FaeI CATG 7 cut(s) 19, 404, 491, 539, 590, 652, 816
FalI AAGNNNNNCTT 2 cut(s) 432, 464
FaqI GGGAC 2 cut(s) 572, 900
FatI CATG 7 cut(s) 15, 400, 487, 535, 586, 648, 812
FauI CCCGC 3 cut(s) 66, 184, 194
FbaI TGATCA 1 cut(s) 343
FblI GTMKAC 1 cut(s) 498
Fnu4HI GCNGC 5 cut(s) 265, 268, 807, 810, 924
FokI GGATG 4 cut(s) 691, 759, 814, 927
FriOI GRGCYC 1 cut(s) 161
Fsp4HI GCNGC 5 cut(s) 265, 268, 807, 810, 924
FspBI CTAG 2 cut(s) 504, 771
FspI TGCGCA 1 cut(s) 571
GlaI GCGC 1 cut(s) 571
GluI GCNGC 5 cut(s) 265, 268, 807, 810, 924
HaeIII GGCC 4 cut(s) 50, 196, 683, 720
HgaI GACGC 1 cut(s) 318
HhaI GCGC 1 cut(s) 572
Hin1II CATG 7 cut(s) 19, 404, 491, 539, 590, 652, 816
Hin6I GCGC 1 cut(s) 570
HinP1I GCGC 1 cut(s) 570
HincII GTYRAC 2 cut(s) 697, 768
HindII GTYRAC 2 cut(s) 697, 768
HindIII AAGCTT 2 cut(s) 224, 605
HinfI GANTC 5 cut(s) 164, 482, 550, 739, 851
HpaI GTTAAC 2 cut(s) 697, 768
HphI GGTGA 2 cut(s) 263, 992
Hpy166II GTNNAC 6 cut(s) 124, 131, 308, 499, 697, 768
Hpy188I TCNGA 2 cut(s) 387, 525
Hpy188III TCNNGA 5 cut(s) 536, 794, 848, 861, 978
Hpy8I GTNNAC 6 cut(s) 124, 131, 308, 499, 697, 768
HpyAV CCTTC 6 cut(s) 173, 284, 434, 458, 731, 971
HpyCH4III ACNGT 3 cut(s) 128, 232, 754
HpyCH4IV ACGT 1 cut(s) 971
HpyCH4V TGCA 4 cut(s) 19, 517, 586, 671
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 21
HpyF3I CTNAG 4 cut(s) 222, 386, 687, 927
HpySE526I ACGT 1 cut(s) 971
Hsp92II CATG 7 cut(s) 19, 404, 491, 539, 590, 652, 816
HspAI GCGC 1 cut(s) 570
Ksp22I TGATCA 1 cut(s) 343
KspAI GTTAAC 2 cut(s) 697, 768
Kzo9I GATC 6 cut(s) 94, 253, 343, 510, 596, 980
LguI GCTCTTC 1 cut(s) 387
LmnI GCTCC 2 cut(s) 449, 840
Lsp1109I GCAGC 3 cut(s) 276, 821, 910
LweI GCATC 1 cut(s) 878
MaeI CTAG 2 cut(s) 504, 771
MaeII ACGT 1 cut(s) 971
MaeIII GTNAC 2 cut(s) 232, 433
MalI GATC 6 cut(s) 96, 255, 345, 512, 598, 982
MbiI CCGCTC 1 cut(s) 201
MboI GATC 6 cut(s) 94, 253, 343, 510, 596, 980
MboII GAAGA 6 cut(s) 116, 248, 363, 374, 524, 966
MflI RGATCY 3 cut(s) 94, 510, 596
MhlI GDGCHC 1 cut(s) 161
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 5 cut(s) 52, 174, 238, 294, 819
MluNI TGGCCA 1 cut(s) 50
MlyI GAGTC 2 cut(s) 158, 748
MmeI TCCRAC 1 cut(s) 738
MnlI CCTC 6 cut(s) 483, 682, 769, 849, 859, 975
Mox20I TGGCCA 1 cut(s) 50
MscI TGGCCA 1 cut(s) 50
MseI TTAA 5 cut(s) 38, 564, 696, 767, 900
MslI CAYNNNNRTG 1 cut(s) 591
Msp20I TGGCCA 1 cut(s) 50
MspA1I CMGCKG 2 cut(s) 267, 728
MspR9I CCNGG 1 cut(s) 711
MvaI CCWGG 1 cut(s) 711
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 2 cut(s) 16, 21
NdeII GATC 6 cut(s) 94, 253, 343, 510, 596, 980
NlaIII CATG 7 cut(s) 19, 404, 491, 539, 590, 652, 816
NlaIV GGNNCC 5 cut(s) 195, 445, 684, 780, 846
NmeAIII GCCGAG 2 cut(s) 222, 240
NmuCI GTSAC 1 cut(s) 232
NsbI TGCGCA 1 cut(s) 571
NspI RCATGY 5 cut(s) 19, 491, 590, 652, 816
NspV TTCGAA 1 cut(s) 451
PaeI GCATGC 2 cut(s) 19, 590
PagI TCATGA 1 cut(s) 535
PciI ACATGT 1 cut(s) 648
PciSI GCTCTTC 1 cut(s) 387
PfeI GAWTC 3 cut(s) 482, 550, 851
PflMI CCANNNNNTGG 1 cut(s) 716
PkrI GCNGC 5 cut(s) 266, 269, 808, 811, 925
PleI GAGTC 2 cut(s) 158, 747
PpsI GAGTC 2 cut(s) 158, 747
PscI ACATGT 1 cut(s) 648
Psp6I CCWGG 1 cut(s) 709
PspGI CCWGG 1 cut(s) 709
PspN4I GGNNCC 5 cut(s) 195, 445, 684, 780, 846
PspPI GGNCC 2 cut(s) 194, 682
PsuI RGATCY 3 cut(s) 94, 510, 596
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 2 cut(s) 125, 751
RsaNI GTAC 2 cut(s) 124, 750
RseI CAYNNNNRTG 1 cut(s) 591
SapI GCTCTTC 1 cut(s) 387
SaqAI TTAA 5 cut(s) 38, 564, 696, 767, 900
SatI GCNGC 5 cut(s) 265, 268, 807, 810, 924
Sau3AI GATC 6 cut(s) 94, 253, 343, 510, 596, 980
Sau96I GGNCC 2 cut(s) 194, 682
SchI GAGTC 2 cut(s) 158, 748
ScrFI CCNGG 1 cut(s) 711
SduI GDGCHC 1 cut(s) 161
SfaNI GCATC 1 cut(s) 878
SfuI TTCGAA 1 cut(s) 451
SmiMI CAYNNNNRTG 1 cut(s) 591
SphI GCATGC 2 cut(s) 19, 590
Sse9I AATT 5 cut(s) 52, 174, 238, 294, 819
SsiI CCGC 6 cut(s) 73, 191, 201, 267, 611, 806
SspI AATATT 1 cut(s) 577
SspMI CTAG 2 cut(s) 504, 771
StyD4I CCNGG 1 cut(s) 709
StyI CCWWGG 1 cut(s) 661
TaaI ACNGT 3 cut(s) 128, 232, 754
TaiI ACGT 1 cut(s) 974
TaqI TCGA 4 cut(s) 102, 167, 451, 886
TasI AATT 5 cut(s) 52, 174, 238, 294, 819
TatI WGTACW 2 cut(s) 123, 749
TauI GCSGC 2 cut(s) 270, 809
TfiI GAWTC 3 cut(s) 482, 550, 851
Tru1I TTAA 5 cut(s) 38, 564, 696, 767, 900
Tru9I TTAA 5 cut(s) 38, 564, 696, 767, 900
TscAI CASTG 1 cut(s) 925
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 3 cut(s) 264, 809, 923
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 3 cut(s) 303, 304, 524
TspGWI ACGGA 1 cut(s) 933
TspRI CASTG 1 cut(s) 925
Van91I CCANNNNNTGG 1 cut(s) 716
XceI RCATGY 5 cut(s) 19, 491, 590, 652, 816
XcmI CCANNNNNNNNNTGG 1 cut(s) 732
XmiI GTMKAC 1 cut(s) 498
XspI CTAG 2 cut(s) 504, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.