RLG00000036822

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000243
Physical Location & Seq
Reverse (-)
85231517 .. 85237551
6035 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036822

Sequence Viewer

Length: 2127 bp
ATGGAACTCACGCCTGGAGGTGGCGTTGCCGCCGGCCCGGTTGCTTCTTCCTCTTATTCATCATCACCTGTTCAGTGGAAATATGATTCACCTGTTCAGTGGAAGTATGATGTGTTCTTGAGTTTCAGGGGTCCAGACACTCGAAGGAGTATCACATCTTATCTATACGATCGACTGAAGAGGAGAGGAATTAAAACATTCATGGATGATCCAGATCTTCAAGTAGGGGATGTGATATCTCCCGCTCTCCTAAAGGCAATTGAAGGATCAAGGTTTGCAATTGTTGTTCTGTCGGAAAACTATGCTCATTCTACTTGGTGTTCGGAGGAACTTACAAAGATTTGTGAGTGCATGGAAGGAAGCAACAGAATTTTTCCGCTTGTTTATAAAGTGGAACCATCTGATGTACGGTACATTACGAAGAGTTTCGGAGAAGCTTTTAATAAGCATGCAAGCTATGGGCGACACACATTACAGAATGTGCGGCGGTGGAATGATGCTTTAAAAAAAGTGTCCAGTTTCTCTGGGTGGGATACAAAGAATTACAAGACTGATATAGAACTTGTAGACGCAATTGTGGATTTTGTGTGCAATAAAATACAACCTATTGACACTGAGTTTACAATGTCTTCCGGAGATTTTGAAGCATATGAAGCAACAAGAAAAGCCATAGATGAGGTTATGAAGGCGCTAAAAGATAAAGATGACGAGGTCACTGCGGATGGTGTGGGAGGCATCGGCAAGACAACCATGGTAAAACACATCGCTGCACAATCCTGCAAAAATGGGACTTCTCATCATGTGATTATGACTGTTGTGTCCCAGAGCCCTGACTTGACAAAAATTCAAGGCACATTGGCGGGGCCGAGCGGGTGTTGTACTGCGAATAGCAGTTGCAGTGCAAGTGTCAGAATTAAGGGAGCGCGGCCGCGGGGGCTGAAGCCGCCGCCGTCGATGGTGAGGGTTCCAGTGATGGACAATGGATGCTCGACTTGGGACATTTTGAGGGACTCTGCGCCGGAAGAAGAGAGGGAGTCGTCGGAAATGGTGCAGAGAAGGTTTGGTTGCTTTTCCTCGTCTTCTTCTGATGAAGAAGAAGAACTAGAAGAAGAAGTTGAAGAAGGAGAGGATGTTGACTTTTCCAGCACTACTGCAGAGATTTCCAGTATTTCTCCAAATGGAAGGTTTTGGCCGGATGAGAAGTTTAAGCTTACTGTCATCACGAATTGGGAAAAGGGTAGTTTTCTTGGCAGCGGCTCTTTTGGGTCTGTGTATGAAGGAATTTCTGATGACGGATGCTTCTTTGCTGTCAAGGAAGTTTCCTTGCTTGATCAAGGAAGGCTGGGGAGGCAAAGAGTTTTTCAACTTGAACAGGAGATTGCTCTTCTGAGTCAGTTTGAACATGAGAACATAGTTCAGTATTATGGCACACAAAAGGATGAGTCAAAGCTCTATATCTTTCTGGAACTTGTAACTAAAGGCTCCCTTCTAAAGCTATATCAAACGTACCATCTTACAGATTCGCATGTCTCTGTATACACTAGACAGATCTTGCAAGGTCTGAAGTATCTTCACGACCGAAAAGTGATTCACAGGGACATTAAATGCGCAAATCTTTTGGTGAATGTTAATGGATCTGTGAAGCTTGCTGACTTTGGATTGGCAAAGACTATCAAAATGAACAACATAAAATCTTGCCAAGGAACTGCACACTGGATGGCCCCTGAGGTTGTTAATAGTAAGAGTCAAGGTTATGGCCTTCCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTGGGATGGTTCCATACTCTAATCTCAAACCGATGCAGGCATTATTGAAAATTGGAAAGGGGGTGCCCCCTCTGGTTCCTGATTCTCTTTCAAAAGAGGCACAAGATTTCATCCGTCTATGCTTACAACCTATAAGTTGTGTTGGTAATAAGCTCTATTCGACCCAAAGAAGCAGGATGGCAACTCATCCATGTTCTACAATTTGCTTTGGAATCCCGTGTTCAGCCATAAAATCTTGCCTTACGCTGGTGGAGTGCAATGCTTGTTATAACTTCAGTGATTTTCAAGGTCTACGCCAGATCTGGCAATGGGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

709

Amino Acids

78.83

Weight (kDa)

5.91

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 35 - 206 3.5e-44 TIR domain
TIR_2 PF13676 38 - 139 2.7e-16 TIR domain
Pkinase PF00069 411 - 645 3.3e-61 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 414 - 645 4.4e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 387, 2076
AasI GACNNNNNNGTC 1 cut(s) 817
Acc16I TGCGCA 1 cut(s) 1609
AccB1I GGYRCC 1 cut(s) 1870
AccBSI CCGCTC 2 cut(s) 245, 870
AccI GTMKAC 3 cut(s) 567, 1536, 2098
AccII CGCG 2 cut(s) 925, 931
AccIII TCCGGA 1 cut(s) 632
AclWI GGATC 3 cut(s) 203, 274, 1642
AcoI YGGCCR 2 cut(s) 926, 1190
AcsI RAATTY 3 cut(s) 369, 843, 1281
AcuI CTGAAG 4 cut(s) 197, 959, 1583, 2065
AfaI GTAC 5 cut(s) 408, 413, 880, 1508, 1789
AfiI CCNNNNNNNGG 2 cut(s) 20, 2053
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 8 cut(s) 437, 456, 1210, 1450, 1495, 1645, 1766, 1960
AluI AGCT 8 cut(s) 437, 456, 1210, 1450, 1495, 1645, 1766, 1960
Alw26I GTCTC 1 cut(s) 1534
AlwI GGATC 3 cut(s) 203, 274, 1642
Aor13HI TCCGGA 1 cut(s) 632
AoxI GGCC 6 cut(s) 34, 863, 926, 1190, 1719, 1756
ApeKI GCWGC 2 cut(s) 767, 1251
ApoI RAATTY 3 cut(s) 369, 843, 1281
Asp700I GAANNNNTTC 1 cut(s) 425
AspLEI GCGC 4 cut(s) 691, 925, 1018, 1610
AspS9I GGNCC 4 cut(s) 35, 131, 863, 1720
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 4 cut(s) 57, 81, 970, 1633
AvaII GGWCC 1 cut(s) 131
AxyI CCTNAGG 1 cut(s) 1725
BaeGI GKGCMC 1 cut(s) 1875
BanI GGYRCC 1 cut(s) 1870
BanII GRGCYC 1 cut(s) 830
BbsI GAAGAC 2 cut(s) 621, 1071
BbvI GCAGC 2 cut(s) 754, 1263
BccI CCATC 8 cut(s) 406, 716, 949, 967, 1518, 1711, 1807, 1978
BceAI ACGGC 1 cut(s) 934
BcgI CGANNNNNNTGC 2 cut(s) 698, 732
BciT130I CCWGG 1 cut(s) 15
BciVI GTATCC 1 cut(s) 526
BclI TGATCA 1 cut(s) 1330
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 1534
BfaI CTAG 2 cut(s) 1103, 1542
BfmI CTRYAG 1 cut(s) 1152
BfoI RGCGCY 1 cut(s) 692
BfuI GTATCC 1 cut(s) 526
BglI GCCNNNNNGGC 1 cut(s) 934
BglII AGATCT 3 cut(s) 214, 1548, 2106
BisI GCNGC 9 cut(s) 30, 485, 768, 926, 929, 944, 947, 1252, 1255
BlsI GCNGC 9 cut(s) 31, 486, 769, 927, 930, 945, 948, 1253, 1256
Bme1390I CCNGG 2 cut(s) 15, 38
Bme18I GGWCC 1 cut(s) 131
BmgT120I GGNCC 4 cut(s) 35, 131, 863, 1720
BmiI GGNNCC 9 cut(s) 132, 396, 864, 966, 1483, 1722, 1818, 1872, 1884
BmrFI CCNGG 2 cut(s) 15, 38
BmrI ACTGGG 1 cut(s) 1818
BmsI GCATC 5 cut(s) 487, 744, 974, 1286, 1830
BmuI ACTGGG 1 cut(s) 1818
BpiI GAAGAC 2 cut(s) 621, 1071
BpmI CTGGAG 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 139
BpuMI CCSGG 1 cut(s) 38
BsaBI GATNNNNATC 1 cut(s) 213
BsaJI CCNNGG 3 cut(s) 750, 929, 1699
BsaWI WCCGGW 1 cut(s) 632
BsaXI ACNNNNNCTCC 4 cut(s) 9, 39, 1116, 1146
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 2053
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 5 cut(s) 516, 968, 1164, 1718, 1813
Bse21I CCTNAGG 1 cut(s) 1725
Bse3DI GCAATG 2 cut(s) 2071, 2120
Bse8I GATNNNNATC 1 cut(s) 213
BseAI TCCGGA 1 cut(s) 632
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 3 cut(s) 750, 929, 1699
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 2 cut(s) 20, 2053
BseMI GCAATG 2 cut(s) 2071, 2120
BseMII CTCAG 3 cut(s) 606, 1379, 1716
BseNI ACTGG 5 cut(s) 516, 968, 1164, 1718, 1813
BseRI GAGGAG 1 cut(s) 196
BseSI GKGCMC 1 cut(s) 1875
BseX3I CGGCCG 1 cut(s) 926
BseXI GCAGC 2 cut(s) 754, 1263
BseYI CCCAGC 1 cut(s) 1342
BsgI GTGCAG 3 cut(s) 753, 1070, 1692
Bsh1236I CGCG 2 cut(s) 925, 931
Bsh1285I CGRYCG 3 cut(s) 172, 929, 1579
BshFI GGCC 6 cut(s) 36, 865, 928, 1192, 1721, 1758
BshNI GGYRCC 1 cut(s) 1870
BsiEI CGRYCG 3 cut(s) 172, 929, 1579
BsiSI CCGG 5 cut(s) 33, 38, 633, 1019, 1193
BslFI GGGAC 5 cut(s) 802, 805, 1010, 1022, 1610
BslI CCNNNNNNNGG 2 cut(s) 20, 2053
BsmAI GTCTC 1 cut(s) 1534
BsmFI GGGAC 5 cut(s) 802, 805, 1010, 1022, 1610
BsnI GGCC 6 cut(s) 36, 865, 928, 1192, 1721, 1758
Bsp1286I GDGCHC 2 cut(s) 830, 1875
Bsp13I TCCGGA 1 cut(s) 632
Bsp143I GATC 8 cut(s) 169, 208, 214, 266, 1330, 1548, 1634, 2106
Bsp19I CCATGG 1 cut(s) 750
BspANI GGCC 6 cut(s) 36, 865, 928, 1192, 1721, 1758
BspCNI CTCAG 3 cut(s) 607, 1380, 1717
BspEI TCCGGA 1 cut(s) 632
BspFNI CGCG 2 cut(s) 925, 931
BspLI GGNNCC 9 cut(s) 132, 396, 864, 966, 1483, 1722, 1818, 1872, 1884
BspMAI CTGCAG 1 cut(s) 1156
BspPI GGATC 3 cut(s) 203, 274, 1642
BspQI GCTCTTC 1 cut(s) 1389
BspT107I GGYRCC 1 cut(s) 1870
BsrBI CCGCTC 2 cut(s) 245, 870
BsrDI GCAATG 2 cut(s) 2071, 2120
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 5 cut(s) 516, 968, 1164, 1718, 1813
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 3 cut(s) 750, 929, 1699
BssMI GATC 8 cut(s) 169, 208, 214, 266, 1330, 1548, 1634, 2106
BssNAI GTATAC 1 cut(s) 1537
BssT1I CCWWGG 2 cut(s) 750, 1699
Bst1107I GTATAC 1 cut(s) 1537
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 4 cut(s) 411, 814, 1216, 1792
Bst6I CTCTTC 4 cut(s) 173, 416, 1020, 1389
BstC8I GCNNGC 5 cut(s) 34, 450, 454, 1647, 1845
BstDEI CTNAG 3 cut(s) 615, 1388, 1725
BstDSI CCRYGG 2 cut(s) 750, 929
BstFNI CGCG 2 cut(s) 925, 931
BstH2I RGCGCY 1 cut(s) 692
BstHHI GCGC 4 cut(s) 691, 925, 1018, 1610
BstKTI GATC 8 cut(s) 172, 211, 217, 269, 1333, 1551, 1637, 2109
BstMAI GTCTC 1 cut(s) 1534
BstMBI GATC 8 cut(s) 169, 208, 214, 266, 1330, 1548, 1634, 2106
BstMCI CGRYCG 3 cut(s) 172, 929, 1579
BstMWI GCNNNNNNNGC 4 cut(s) 653, 934, 943, 1348
BstNI CCWGG 1 cut(s) 15
BstNSI RCATGY 2 cut(s) 452, 1529
BstSCI CCNGG 2 cut(s) 13, 36
BstSFI CTRYAG 1 cut(s) 1152
BstSLI GKGCMC 1 cut(s) 1875
BstUI CGCG 2 cut(s) 925, 931
BstV1I GCAGC 2 cut(s) 754, 1263
BstV2I GAAGAC 2 cut(s) 621, 1071
BstX2I RGATCY 4 cut(s) 214, 1548, 1634, 2106
BstYI RGATCY 4 cut(s) 214, 1548, 1634, 2106
BstZ17I GTATAC 1 cut(s) 1537
BstZI CGGCCG 1 cut(s) 926
Bsu36I CCTNAGG 1 cut(s) 1725
BsuI GTATCC 1 cut(s) 526
BsuRI GGCC 6 cut(s) 36, 865, 928, 1192, 1721, 1758
BtgI CCRYGG 2 cut(s) 750, 929
BtgZI GCGATG 1 cut(s) 748
BtsI GCAGTG 2 cut(s) 714, 904
BtsIMutI CAGTG 8 cut(s) 80, 104, 612, 714, 904, 975, 1711, 2089
Cac8I GCNNGC 5 cut(s) 34, 450, 454, 1647, 1845
CciNI GCGGCCGC 1 cut(s) 926
CfoI GCGC 4 cut(s) 691, 925, 1018, 1610
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 4 cut(s) 35, 131, 863, 1720
Cfr42I CCGCGG 1 cut(s) 932
CseI GACGC 1 cut(s) 578
Csp6I GTAC 5 cut(s) 407, 412, 879, 1507, 1788
CviAII CATG 8 cut(s) 202, 352, 449, 751, 800, 1403, 1526, 1998
CviQI GTAC 5 cut(s) 407, 412, 879, 1507, 1788
DdeI CTNAG 3 cut(s) 615, 1388, 1725
DpnI GATC 8 cut(s) 171, 210, 216, 268, 1332, 1550, 1636, 2108
DpnII GATC 8 cut(s) 169, 208, 214, 266, 1330, 1548, 1634, 2106
DraI TTTAAA 1 cut(s) 504
DrdI GACNNNNNNGTC 1 cut(s) 817
DseDI GACNNNNNNGTC 1 cut(s) 817
EaeI YGGCCR 2 cut(s) 926, 1190
EagI CGGCCG 1 cut(s) 926
Eam1104I CTCTTC 4 cut(s) 173, 416, 1020, 1389
EarI CTCTTC 4 cut(s) 173, 416, 1020, 1389
EclXI CGGCCG 1 cut(s) 926
Eco130I CCWWGG 2 cut(s) 750, 1699
Eco24I GRGCYC 1 cut(s) 830
Eco32I GATATC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 131
Eco52I CGGCCG 1 cut(s) 926
Eco57I CTGAAG 4 cut(s) 197, 959, 1583, 2065
Eco81I CCTNAGG 1 cut(s) 1725
EcoRII CCWGG 1 cut(s) 13
EcoRV GATATC 1 cut(s) 237
EcoT14I CCWWGG 2 cut(s) 750, 1699
EcoT38I GRGCYC 1 cut(s) 830
ErhI CCWWGG 2 cut(s) 750, 1699
FaeI CATG 8 cut(s) 205, 355, 452, 754, 803, 1406, 1529, 2001
FalI AAGNNNNNCTT 2 cut(s) 1470, 1502
FaqI GGGAC 5 cut(s) 802, 805, 1010, 1022, 1610
FatI CATG 8 cut(s) 201, 351, 448, 750, 799, 1402, 1525, 1997
FauI CCCGC 4 cut(s) 250, 853, 863, 924
FauNDI CATATG 1 cut(s) 649
FbaI TGATCA 1 cut(s) 1330
FblI GTMKAC 3 cut(s) 567, 1536, 2098
Fnu4HI GCNGC 9 cut(s) 30, 485, 768, 926, 929, 944, 947, 1252, 1255
FriOI GRGCYC 1 cut(s) 830
Fsp4HI GCNGC 9 cut(s) 30, 485, 768, 926, 929, 944, 947, 1252, 1255
FspBI CTAG 2 cut(s) 1103, 1542
FspI TGCGCA 1 cut(s) 1609
GlaI GCGC 4 cut(s) 690, 924, 1017, 1609
GluI GCNGC 9 cut(s) 30, 485, 768, 926, 929, 944, 947, 1252, 1255
GsaI CCCAGC 1 cut(s) 1346
GsuI CTGGAG 1 cut(s) 36
HaeII RGCGCY 1 cut(s) 692
HaeIII GGCC 6 cut(s) 36, 865, 928, 1192, 1721, 1758
HapII CCGG 5 cut(s) 33, 38, 633, 1019, 1193
HgaI GACGC 1 cut(s) 578
HhaI GCGC 4 cut(s) 691, 925, 1018, 1610
Hin1II CATG 8 cut(s) 205, 355, 452, 754, 803, 1406, 1529, 2001
Hin6I GCGC 4 cut(s) 689, 923, 1016, 1608
HinP1I GCGC 4 cut(s) 689, 923, 1016, 1608
HincII GTYRAC 2 cut(s) 1135, 1806
HindII GTYRAC 2 cut(s) 1135, 1806
HindIII AAGCTT 3 cut(s) 435, 1208, 1643
HpaI GTTAAC 1 cut(s) 1806
HpaII CCGG 5 cut(s) 33, 38, 633, 1019, 1193
HphI GGTGA 4 cut(s) 57, 81, 970, 1633
Hpy166II GTNNAC 6 cut(s) 568, 621, 1135, 1537, 1806, 2099
Hpy188III TCNNGA 8 cut(s) 118, 134, 212, 633, 1222, 1463, 1574, 1886
Hpy8I GTNNAC 6 cut(s) 568, 621, 1135, 1537, 1806, 2099
Hpy99I CGWCG 2 cut(s) 955, 1042
HpyCH4III ACNGT 4 cut(s) 411, 814, 1216, 1792
HpyCH4IV ACGT 1 cut(s) 1505
HpyF10VI GCNNNNNNNGC 4 cut(s) 653, 934, 943, 1348
HpyF3I CTNAG 3 cut(s) 615, 1388, 1725
HpySE526I ACGT 1 cut(s) 1505
Hsp92II CATG 8 cut(s) 205, 355, 452, 754, 803, 1406, 1529, 2001
HspAI GCGC 4 cut(s) 689, 923, 1016, 1608
Kpn2I TCCGGA 1 cut(s) 632
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
Ksp22I TGATCA 1 cut(s) 1330
KspAI GTTAAC 1 cut(s) 1806
KspI CCGCGG 1 cut(s) 932
Kzo9I GATC 8 cut(s) 169, 208, 214, 266, 1330, 1548, 1634, 2106
LguI GCTCTTC 1 cut(s) 1389
LmnI GCTCC 2 cut(s) 920, 1487
Lsp1109I GCAGC 2 cut(s) 754, 1263
LweI GCATC 5 cut(s) 487, 744, 974, 1286, 1830
MaeI CTAG 2 cut(s) 1103, 1542
MaeII ACGT 1 cut(s) 1505
MaeIII GTNAC 2 cut(s) 712, 1471
MalI GATC 8 cut(s) 171, 210, 216, 268, 1332, 1550, 1636, 2108
MbiI CCGCTC 2 cut(s) 245, 870
MboI GATC 8 cut(s) 169, 208, 214, 266, 1330, 1548, 1634, 2106
MfeI CAATTG 3 cut(s) 258, 279, 573
MflI RGATCY 4 cut(s) 214, 1548, 1634, 2106
MhlI GDGCHC 2 cut(s) 830, 1875
MlyI GAGTC 6 cut(s) 1004, 1043, 1399, 1451, 1753, 1786
MmeI TCCRAC 3 cut(s) 273, 1020, 1776
MroI TCCGGA 1 cut(s) 632
MroNI GCCGGC 1 cut(s) 32
MroXI GAANNNNTTC 1 cut(s) 425
MseI TTAA 9 cut(s) 192, 441, 503, 915, 1206, 1602, 1629, 1734, 1805
MspA1I CMGCKG 3 cut(s) 931, 1254, 1766
MspI CCGG 5 cut(s) 33, 38, 633, 1019, 1193
MspR9I CCNGG 2 cut(s) 15, 38
MunI CAATTG 3 cut(s) 258, 279, 573
MvaI CCWGG 1 cut(s) 15
MvnI CGCG 2 cut(s) 925, 931
MwoI GCNNNNNNNGC 4 cut(s) 653, 934, 943, 1348
NaeI GCCGGC 1 cut(s) 34
NciI CCSGG 1 cut(s) 38
NcoI CCATGG 1 cut(s) 750
NdeI CATATG 1 cut(s) 649
NdeII GATC 8 cut(s) 169, 208, 214, 266, 1330, 1548, 1634, 2106
NgoMIV GCCGGC 1 cut(s) 32
NlaIII CATG 8 cut(s) 205, 355, 452, 754, 803, 1406, 1529, 2001
NlaIV GGNNCC 9 cut(s) 132, 396, 864, 966, 1483, 1722, 1818, 1872, 1884
NmeAIII GCCGAG 1 cut(s) 891
NmuCI GTSAC 1 cut(s) 712
NotI GCGGCCGC 1 cut(s) 926
NsbI TGCGCA 1 cut(s) 1609
NspI RCATGY 2 cut(s) 452, 1529
PaeI GCATGC 1 cut(s) 452
PciSI GCTCTTC 1 cut(s) 1389
PdiI GCCGGC 1 cut(s) 34
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 5 cut(s) 86, 1520, 1588, 1889, 2019
PflFI GACNNNGTC 1 cut(s) 710
PkrI GCNGC 9 cut(s) 31, 486, 769, 927, 930, 945, 948, 1253, 1256
Ple19I CGATCG 1 cut(s) 172
PleI GAGTC 6 cut(s) 1004, 1042, 1398, 1450, 1752, 1785
PpsI GAGTC 6 cut(s) 1004, 1042, 1398, 1450, 1752, 1785
PsiI TTATAA 2 cut(s) 387, 2076
Psp6I CCWGG 1 cut(s) 13
PspFI CCCAGC 1 cut(s) 1342
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 9 cut(s) 132, 396, 864, 966, 1483, 1722, 1818, 1872, 1884
PspPI GGNCC 4 cut(s) 35, 131, 863, 1720
PsrI GAACNNNNNNTAC 2 cut(s) 98, 130
PstI CTGCAG 1 cut(s) 1156
PsuI RGATCY 4 cut(s) 214, 1548, 1634, 2106
PsyI GACNNNGTC 1 cut(s) 710
PvuI CGATCG 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 1766
RsaI GTAC 5 cut(s) 408, 413, 880, 1508, 1789
RsaNI GTAC 5 cut(s) 407, 412, 879, 1507, 1788
SacII CCGCGG 1 cut(s) 932
SapI GCTCTTC 1 cut(s) 1389
SaqAI TTAA 9 cut(s) 192, 441, 503, 915, 1206, 1602, 1629, 1734, 1805
SatI GCNGC 9 cut(s) 30, 485, 768, 926, 929, 944, 947, 1252, 1255
Sau3AI GATC 8 cut(s) 169, 208, 214, 266, 1330, 1548, 1634, 2106
Sau96I GGNCC 4 cut(s) 35, 131, 863, 1720
SchI GAGTC 6 cut(s) 1004, 1043, 1399, 1451, 1753, 1786
ScrFI CCNGG 2 cut(s) 15, 38
SduI GDGCHC 2 cut(s) 830, 1875
SfaNI GCATC 5 cut(s) 487, 744, 974, 1286, 1830
SfcI CTRYAG 1 cut(s) 1152
Sfr303I CCGCGG 1 cut(s) 932
SgrBI CCGCGG 1 cut(s) 932
SinI GGWCC 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
SphI GCATGC 1 cut(s) 452
SspMI CTAG 2 cut(s) 1103, 1542
StyD4I CCNGG 2 cut(s) 13, 36
StyI CCWWGG 2 cut(s) 750, 1699
TaaI ACNGT 4 cut(s) 411, 814, 1216, 1792
TaiI ACGT 1 cut(s) 1508
TaqI TCGA 5 cut(s) 142, 172, 953, 989, 1967
TaqII GACCGA 1 cut(s) 1593
TatI WGTACW 2 cut(s) 878, 1787
TauI GCSGC 7 cut(s) 32, 487, 928, 931, 946, 949, 1257
TfiI GAWTC 5 cut(s) 86, 1520, 1588, 1889, 2019
Tru1I TTAA 9 cut(s) 192, 441, 503, 915, 1206, 1602, 1629, 1734, 1805
Tru9I TTAA 9 cut(s) 192, 441, 503, 915, 1206, 1602, 1629, 1734, 1805
TscAI CASTG 8 cut(s) 80, 104, 619, 721, 904, 975, 1718, 2089
TseFI GTSAC 1 cut(s) 712
TseI GCWGC 2 cut(s) 767, 1251
Tsp45I GTSAC 1 cut(s) 712
TspDTI ATGAA 8 cut(s) 48, 190, 666, 698, 1104, 1290, 1694, 1906
TspGWI ACGGA 2 cut(s) 1308, 1910
TspRI CASTG 8 cut(s) 80, 104, 619, 721, 904, 975, 1718, 2089
Tth111I GACNNNGTC 1 cut(s) 710
VpaK11BI GGWCC 1 cut(s) 131
XapI RAATTY 3 cut(s) 369, 843, 1281
XceI RCATGY 2 cut(s) 452, 1529
XcmI CCANNNNNNNNNTGG 1 cut(s) 1770
XmiI GTMKAC 3 cut(s) 567, 1536, 2098
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 2 cut(s) 1103, 1542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.